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A Study Of The Synthesis Of Cellulose By The Bacteria Komagataeibacter Xylinus (K. Xylinus) When Under The Action Of An Electric Field In A Microfluidic Reactor, Sindora Baddam

All Theses

Dielectrophoresis (DEP) is an established technique used as a label-free method to manipulate microorganisms by exploiting the interaction of targeted microorganisms with a non-uniform electric field. The strength and direction of the DEP force on a targeted cell, specifically Komagataeibacter xylinus (K. xylinus), is dependent on the frequency of the applied electric field as well as the dielectric properties of the bacterial cell and suspending media.

K. xylinus is a microorganism that can synthesize a fibrous, web-shaped organic material called bacterial cellulose (BC), which contains desirable properties such as high mechanical strength and increased water holding capabilities. By …

Development Of Deterioration Models For Street Pavement In Dallas-Fort Worth Metroplex, Mladjan John Grujicic

Civil Engineering Dissertations - Archive

Accurate prediction of pavement deterioration is vital for an efficient and cost-effective allocation of available budgets for keeping an agency’s road networks operating at a desirable level. Currently, most cities in the Dallas-Fort Worth Metroplex area are using the software PAVERTM and the associated performance models to predict future conditions as they do not have available reliable prediction models. However, the problem with this type of modeling is that the models are not calibrated to local conditions. The Pavement Deterioration Prediction models that have been developed in this research will help any pavement management agencies within DFW Metroplex area to …

Electron-Rich Alkynes And Azodicarboxylates Chemistry Towards Vicinal Diamine Motif Containing Small Molecules And N-Heterocycles, Chandima Jeewantha Narangoda

All Dissertations

Heterocyclic molecules that contain nitrogens and consist of four, five and six-membered rings such as azetidinones (eg: β-lactams), pyrroles, indoles, azoles, triazoles, oxadiazines, and oxadiazinones, etc. are ubiquitous in the use of pharmaceutical fields. This also reflected in the recently FDA approved unique small-molecule pharmaceuticals, where about 90% of those molecules contain a nitrogen heterocycle. Particularly, the vicinal diamino motif has been appeared in leading drug directing candidates such as penicillin, cephalosporins, and oseltamivir (Tamiflu) over the past years.

Historically, there has been minimal interest in synthesizing a broad scope of vicinal diamino motif-containing small molecules and heterocyclic compounds for …

Guanidinium Sulfation Crystallization: Strategy For Enantiomeric Identification And Diastereomeric Resolution, Beau Ryan Brummel

All Dissertations

The main portion of this thesis describes two distinct applications of guanidinium sulfation crystallization: use of a flat guanidinium chloride for enantiomeric identification and a chiral guanidinium bromide for diastereomeric resolution. Additionally, the following two chapters includes the modification of naturally occurring materials for pollution remediation and the synthesis of biodegradable polyesters for biomedical imaging.The importance of chirality can be found in a variety of fields including pharmaceuticals, fragrances, food additives, agrochemicals, and forensics. Most biologically active organic compounds such as amino acids and carbohydrates exhibit chirality, resulting in a high degree of stereoselectivity in processes that are mediated by …

Application Of Genomics In Nursing Practice, Elizabeth Cameron Hassen

All Dissertations

ABSTRACTThis dissertation begins with the introduction of genetics, genomics, and the Healthcare Genetics (HCG) model. The five concepts of the HCG model, genetics/genomics, education, environment, clinical testing and therapeutics, and ethical/legal/social implications (ELSI) are defined. These concepts are threaded throughout the manuscripts and dissertation chapters. Chapter II defines molecular testing and differences between single-gene and multi-gene testing. The Kaplan Meier Curve and its use in research, to illustrate survival over time, is discussed. The chapter provides specific examples of both predictive and prognostic genomic testing used in breast, lung, colon, and prostate cancers. Chapter III examines the application of the …

Recombinant Piriform Spider Silk Production, Cole R. Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider silks are remarkable biomaterials, possessing a variety of desirable mechanical and material properties. Humans have long-sought to utilize these materials. Spiders produce a range of different types of silks for different purposes, such as web-building, egg casing, and prey swathing. Because spiders are difficult to farm, a synthetic biological approach must be used to produce silk-like proteins. This research is intended to improve the production of piriform, a specific type of spider silk. This was done with targeted-improvements to microbial metabolism and recovery methodologies. The methods developed in this research are useful for other types of silk production and …

Triggerable, Targetable, And Trackable Flavonol-Based Carbon Monoxide-Releasing Molecules, Livia S. Lazarus

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Long known as a toxic gas, carbon monoxide (CO) has been recently redefined as a health beneficial molecule produced endogenously in humans. The biological activity of CO has been extensively examined in inflammatory conditions, vasoprotection, anti-apoptotic and anticancer studies, although the mechanisms of action are still not well defined. Because CO is a gaseous molecule, a straightforward administration would be inhalation. However, this delivery method is limited to use in hospital settings under professional supervision. For improved control over the amount and location of CO delivery, metal-free and light-triggered carbon monoxide-releasing molecules are of particular current interest. Our lab is …

Laboratory Scale Anaerobic Reactors For Bioenergy From Wastes: Materials, Mathematical Models, And Stoichiometry, Yehor Pererva

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Anaerobic processes are being explored for the production of bioenergy in the form of biomethane and biohydrogen in a "waste-to-value" approach that considers wastes as a resource. Laboratory scale Biomethane potential (BMP) tests and Up-Flow Anaerobic Sludge Blanket (UASB) reactors can be used to indicate the potential recovery of bioenergy from industry wastes. The goals of this research were to: (1) conduct a critical review of models for determining biomethane potential, (2) develop a theoretical relationship for stoichiometry for biomethane and biohydrogen from wastes, and (3) develop key design aspects for geometry and infrastructure, including gas-liquid-solid separation in laboratory UASB …

Nodulation And Growth Of Shepherdia × Utahensis ‘Torrey’, Ji-Jhong Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Shepherdia ×utahensis ‘Torrey’ (Elaeagnaceae) is a hybrid of two native actinorhizal plants in the Intermountain West, S. argentea (silver buffaloberry) and S. rotundifolia (roundleaf buffaloberry). Due to actinorhizal symbiosis, atmospheric nitrogen (N₂) can be converted to ammonium, a bioavailable form. Actinorhizal plants have great value in sustainable nursery production and urban landscape use. However, nitrogen fertilizer negatively affects the nodulation of actinorhizal plants. As a newly developed hybrid, both the symbiont identity and nodule formation of S. ×utahensis ‘Torrey’ remain largely unknown. Therefore, experiments were conducted to investigate the nodule formation of S. ×utahensis ‘Torrey’ inoculated with field …

Assessment Of Compost On Dryland Wheat Yield And Quality, Soil Fertility And Water Availability In Utah, Kareem A. Adeleke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In 2014-2016 Kareem Adeleke undertook a graduate project under the supervision of Utah State University (USU) Plants, Soils and Climate professors, Drs. Jennifer Reeve, Astrid Jacobson, and Earl Creech. Organic wheat producers face numerous challenges, such as low soil moisture, soil erosion, and low soil fertility. Organic wheat growers generally do not apply fertilizer due to inability to recoup the costs in the short-term. Compost enhances long-term improvement in soil quality, soil fertility and increase yield in low input environments. Understanding of compost carryover effects in dryland wheat systems is necessary for increased yield that will allow adequate supply of …

Influences Of Forest Edges On The Growth And Health Of Old-Growth Coast Redwood Forests, Cody R. Dangerfield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Coast redwood (Sequoia sempervirens) is the tallest species in the world, frequently attaining heights greater than 300 ft. The unique characteristics of the redwoods has led to the establishment of several preservation areas including national and state parks. However, abrupt forests edges created by previous logging and landcover changes has left the remaining stands exposed to elevated temperature, sunlight, and wind intensities, thereby making redwoods along the forest edge more susceptible to windthrow and drought stress. Despite the rarity of old-growth coast redwood forests and their ecological and cultural significance, very few studies have investigated how forests edges …

Classification And Prediction Models For Natural Streamflow Regimes In The Arid Southwestern Usa, Angela M. Merritt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Understanding how natural variation in flow regimes influences stream ecosystem structure and function is critical to the development of effective stream management policies and actions. Spatial variation in flow regimes is well understood for stream reaches in mesic regions, but a more robust characterization of flow regimes in arid regions is needed, especially to support biological monitoring and assessment programs. Methods are specifically needed that can accurately predict the flow regime expected at ungauged reaches. We used long-term (41 y) records of mean daily flow from 287 stream reaches in the arid western USA to develop and compare several alternative …

Electrical Identification Of Innate Immune Cells, Rasha Ayman Nasser

Theses

This thesis is concerned with the electrical characterization of the key players of the innate immune cells. Innate immunity is basically the nonspecific immune response that is triggered by any foreign body that attacks the human system. The key players of the innate immune system are mainly dendritic cells and macrophages. Accurately classifying these cell types helps us understand the mechanism of the immune system thereby enabling the development of models to improve new prospects for therapeutics and diagnostics. The characterization described in this thesis is based on extracting the capacitance for each biological cell using I-V curves. The main …

An Evaluation Of The Trauma-Informed Approach Implemented At A Title I Elementary School, Aleli Vazquez

Dissertations

An Evaluation of the Trauma-Informed Approach Implemented at a Title I Elementary School assesses the merits of the implementation of an intervention to support the social emotional ability and stability of students within elementary schools; called the Trauma Informed Approach. Many children attending elementary schools bring diverse and complex experiences to the classroom setting. Students with multiple adverse childhood experiences attend the educational system. Many students have a more difficult time concentrating and succeeding due to anxiety and lack of physiological needs being met. These needs must be met to secure positive learning gains for these students.

For data gathering …

An Evaluation Of One School District Standards Assessment Program, Derrick King

Dissertations

This project focuses on how one school district uses quarterly assessments to identify standards students have not mastered. This project identifies how some schools utilize quarterly assessment data with varying results. One problem I have identified is a lack of a consistent expectation from district leadership, regarding the expectation for how schools should use the assessment program. In my research, I examine two schools that use the assessment program differently. The literature I present reveals how the program can help improve student achievement. The research reveals what is working well, and what is not. In closing, I recommend implementing a …

Deaf Cultural Capital And Its Conflicts With Hearing Culture: Navigational Successes And Failures, Ashley Greene-Woods, Natalie J. Delgado, Beverly Buchanan, Misty Sides, Abbas Ali Behmanesh, Brian Cheslik, Caroline K. Koo, M. Diane Clark

JADARA

Despite the creation and implementation of laws intended to support and protect Deaf individuals, stories of limited opportunities and oppression within the workplace still exist and are pervasive. Current research in regard to Deaf individuals’ upward mobility includes a discussion of cultural capital, Imposter Syndrome, and navigational capital. To further understand the experiences of Deaf individuals, the research team conducted a mixed-methods study utilizing surveys and interviews. The results provided insight regarding challenges experienced by the participants in either-or-both their education and employment. The data suggests that the use of navigational capital was the most significant predictor for upward mobility.

Epidemiology Of Staphylococcus Aureus Infections In Kenya: Current State, Gaps And Opportunities, Justin Nyasinga, Geoffrey Omuse, John Njenga, Andrew Nyerere, Shima Abdulgader, Mae Newton, Andrew Whitelaw, Gunturu Revathi

Pathology, East Africa

Staphylococcus aureus has maintained its clinical relevance as a major cause of hospital and community acquired infections globally with a high burden of antimicrobial resistance (AMR). Though reported, the burden of infection, antimicrobial resistance and molecular epidemiology of S. aureus are not well defined in Kenya. This descriptive review evaluated reported data on the detection and characterization of S. aureus infections in Kenya. Published data between 2000 and 2020 were evaluated. S. aureus isolation frequencies varied from 1% in blood specimens to 52.6% among skin and soft tissues infections while MRSA rates ranged from 1% to 84.1%. While penicillin resistance …

U.P. Los Baños Journal, Special Issue

U.P. Los Baños Journal

This special issue is dedicated to the memory of Prof. Dwight David Amparo Diestro, who died on June 2, 2020 at the age of 55. Prof. Diestro, a historian of Southern Luzon, was an Associate Professor of history in the Department of Social Sciences (DSS) of UPLB. He was one of the great pillars of DSS, serving as Department Chair for three terms in a total of 10 years. He was the first chair of the Southern Luzon Studies Center and the motivating force behinds its progress.

Study Of Plants Used Against Infections By California Native American Tribes, Maria J. Rojas

Master's Theses

The objectives of this research were to evaluate the antibacterial activity and to determine the chemical composition of a list of medicinal plants used by Native Americans in California. Artemisia californica, Mimulus aurantiacus, Equisetum telmateia, Equisetum hyemale, and Marah fabacea were selected from a list of plants reported as having been used for ailments related to infections by tribes located in California. The extracts obtained through steam distillation from E. telmateia, E. hyemale and M. fabacea were assayed for in vitro antibacterial activity against 16 Gram-negative and 6 Gram-positive bacteria using disk diffusion assays and measuring the diameters of inhibition …

The Promise Of A Multi-Disciplinary, Mixed-Methods Approach To Inform Insect Pest Management: Evidence From Wyoming Alfalfa, Randa Jabbour, Shiri Noy

Faculty Publications

No abstract provided.

Complex Trauma In Childhood And Its Relationship To Emotion Regulation And Distress Tolerance In College Students, Elizabeth Lombardo

USC Aiken Psychology Theses

Objective: The influence of childhood trauma has been found to be related to difficulties in emotion regulation and distress tolerance in young adulthood (Berenz et al., 2018a, 2018b). Research has shown that childhood abuse and adversities such as neglect or emotional abuse results in impaired processes related to the development of emotion regulation and efficient interpersonal skills, while also resulting in symptoms reflecting disordered affective self-regulation (Cloitre et al., 2009; Shipman, Edwards, Brown, Swisher, & Jennings, 2005; Shipman, Zeman, Penza, & Champion, 2000). Research has examined emotional regulation and distress tolerance in the context of childhood trauma but has not …

A New Governance Approach To Regulating Human Genome Editing, John M. Conley, Arlene M. Davis, Gail E. Henderson, Eric T. Juengst, Karen M. Meagher, Rebecca L. Walker, Margaret Waltz, Jean Cadigan

North Carolina Journal of Law & Technology

No abstract provided.

A Theacrine-Based Supplement Increases Cellular Nad+ Levels And Affects Biomarkers Related To Sirtuin Activity In C2c12 Muscle Cells In Vitro, Petey W. Mumford, Shelby C. Osburn, Carlton D. Fox, Joshua S. Godwin, Michael D. Roberts

Faculty Scholarship

There is evidence in rodents to suggest that theacrine-based supplements modulate tissue sirtuin activity as well as other biological processes associated with aging. Herein, we examined if a theacrine-based supplement (termed NAD3) altered sirtuin activity in vitro while also affecting markers of mitochondrial biogenesis. The murine C2C12 myoblast cell line was used for experimentation. Following 7 days of differentiation, myotubes were treated with 0.45 mg/mL of NAD3 (containing ~2 mM theacrine) for 3 and 24 h (n = 6 treatment wells per time point). Relative to control (CTL)-treated cells, NAD3 treatments increased (p < 0.05) Sirt1 mRNA levels at 3 h, as well as global sirtuin activity at 3 and 24 h. Follow-up experiments comparing 24 h NAD3 or CTL treatments indicated that NAD3 increased nicotinamide phosphoribosyltransferase (NAMPT) and SIRT1 protein levels (p < 0.05). Cellular nicotinamide adenine dinucleotide (NAD+) levels were also elevated nearly two-fold after 24 h of NAD3 versus CTL treatments (p < 0.001). Markers of mitochondrial biogenesis were minimally affected. Although these data are limited to select biomarkers in vitro, these preliminary findings suggest that a theacrine-based supplement can modulate select biomarkers related to NAD+ biogenesis and sirtuin activity. However, these changes did not drive increases in mitochondrial biogenesis. While promising, these data are limited to a rodent cell line and human muscle biopsy studies are needed to validate and elucidate the significance of these findings.

Geographic Patterns And Environmental Correlates Of Phylogenetic Relatedness And Diversity For Freshwater Fish Assemblages In North America, Hong Qian, Yong Cao, Daijiang Li, Cindy Chu, Brody Sandel, Xianli Wang

Faculty Publications

© 2020 The Authors. Ecography published by John Wiley & Sons Ltd on behalf of Nordic Society Oikos The tropical niche conservatism hypothesis suggests that most groups should be most phylogenetically clustered in cold, dry environments. This idea has been well-tested in plants and some animal groups, but not for fishes. We assess the geographic patterns of freshwater fish phylogenetic structure and investigate the relationships between these patterns and environmental variables across North America and within two biogeographic realms. Phylogenetic relatedness and diversity of 360 freshwater fish assemblages across North America were quantified with three metrics based on a well-dated …

Reconstructing Genomes Of Carbon Monoxide Oxidisers In Volcanic Deposits Including Members Of The Class Ktedonobacteria, Marcela Hernández, Blanca Vera-Gargallo, Marcela Calabi-Floody, Gary M. King, Ralf Conrad, Christoph C. Tebbe

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Microorganisms can potentially colonise volcanic rocks using the chemical energy in reduced gases such as methane, hydrogen (H2) and carbon monoxide (CO). In this study, we analysed soil metagenomes from Chilean volcanic soils, representing three different successional stages with ages of 380, 269 and 63 years, respectively. A total of 19 metagenome-assembled genomes (MAGs) were retrieved from all stages with a higher number observed in the youngest soil (1640: 2 MAGs, 1751: 1 MAG, 1957: 16 MAGs). Genomic similarity indices showed that several MAGs had amino-acid identity (AAI) values >50% to …

Electro-Optic Satellite Constellation Design Using Multi-Objective Genetic Algorithm, Yasin Tamer

Theses and Dissertations

Satellite constellation design is a complex, highly constrained, and multidisciplinary problem. Unless optimization tools are used, tradeoffs must be conducted at the subsystem level resulting in feasible, but not necessarily optimal, system designs. As satellite technology advances, new methods to optimize the system objectives are developed. This study is based on the development of a representative regional remote sensing constellation design. This thesis analyses the design process of an electrooptic satellite constellation with regional coverage considerations using system-level optimization tools. A multi objective genetic algorithm method is used to optimize the constellation design by utilizing MATLAB and STK integration. Cost, …

Nature-Inspired Topology Optimization Of Recurrent Neural Networks, Abdelrahman A. Elsaid

Theses

Hand-crafting effective and efficient structures for recurrent neural networks (RNNs) is a difficult, expensive, and time-consuming process. To address this challenge, this work presents three nature-inspired (NI) algorithms for neural architecture search (NAS), introducing the subfield of nature-inspired neural architecture search (NI-NAS). These algorithms, based on ant colony optimization (ACO), progress from memory cell structure optimization, to bounded discrete-space architecture optimization, and finally to unbounded continuous-space architecture optimization. These methods were applied to real-world data sets representing challenging engineering problems, such as data from a coal-fired power plant, wind-turbine power generators, and aircraft flight data recorder (FDR) data.

Initial work …

Drag Incorporated: The Homonormative Brand Culture Of Rupaul's Drag Race, Nathan T. Workman

Institute for the Humanities Theses

This thesis argues RuPaul’s Drag Race (RPDR, 2009–) positions itself as a homonormative pathway to LGBTQ+ social inclusion through privileging neoliberal selfbranding and commodity activist practices that reify privileged raced, classed, and sexuality identity markers. Utilizing interdisciplinary and intersectional cultural studies methods to conduct a textual analysis, I examine how RPDR produces homonormative LGBTQ+ identities through the commodification and standardization of drag cultures. In conversation with existing RPDR scholars, I critically survey RPDR’s gender biases and prosocial messaging as an example of brand culture’s reification of hegemony and homonormativity within LGBTQ+ communities. This research considers the …

A Meta-Analysis Of Group Treatment Outcomes For Veterans With Substance Use Disorders, Robert “Tony” Dice

Counseling & Human Services Theses & Dissertations

Group therapy is commonly used in the treatment of substance use disorders (SUD). Many studies exist related to the efficacy of group interventions for veterans with SUDs. A meta-analysis and systematic review of the literature addressing the use of group therapy, specifically psychoeducational groups, cognitive behavioral therapy (CBT) groups and support groups, in the treatment of SUDs with veterans was conducted. The following questions guided the research: What are viable treatment outcomes for psychoeducational, CBT, and support groups of veterans with SUDs? and What are the measures that capture outcomes related to psychoeducational, CBT, and support groups of veterans with …

A Systemic Review And Meta-Analysis Of Psychoeducational Groups For The Treatment Of Psychopathology Resulting From Child Sexual Abuse, Alexis Lynnette Wilkerson

Counseling & Human Services Theses & Dissertations

It is estimated that 16% of men and 26% of women have experienced child sexual abuse (CSA), and studies have shown a strong association with long-term psychosocial consequences. The use of psychoeducational groups with survivors of CSA has been found to produce favorable outcomes; however, no meta-analyses have been conducted on studies assessing the use of psychoeducational groups to treat victims of CSA. The purpose of this systematic review and random-effect meta-analysis was to examine the efficacy of psychoeducational groups for the treatment of psychopathology subsequent to child sexual abuse (CSA) by addressing the following research questions (1) Are psychoeducational …