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Genomic Signatures Of Optimal Growth Temperature In The Family Colwelliaceae, Anais Gentilhomme

Oceanography

The temperature range supporting growth defines a complex physiological phenotype that depends on interactions between an organism's genome and its environment. Its implications are widespread since small changes in optimal growth temperature (OGT) can alter an organism's ability to inhabit an ecological niche. Thus, organisms with extreme thermal growth traits (e.g., psychrophilic, with OGT < 15℃, or thermophilic, with OGT 60 -80℃) may be useful for identifying promising targets when searching for life on other planets, as well as predicting population dynamics in a warming Arctic. We performed comparative genomic analyses of bacteria newly isolated from Arctic sea ice that were affiliated with Colwelliaceae, a family of Gammaproteobacteria that contains many psychrophilic strains, to identify genomic factors that might be used to predict OGT. A phylogenomic analysis of 67 public and 39 newly-sequenced strains, was used to construct an updated phylogenetic tree of Colwelliaceae, of which at least two genera were well represented. To augment the previously reported OGTs of 26 strains, we measured growth rates at −1, 4, 11, and 17 ℃ to determine the OGTs of these 39 new strains of Colwelliaceae. We found that growth rates among all isolates were comparable at −1℃, but varied widely above 10 ℃, indicating higher variability in the ability to tolerate warmer temperatures. To analyze the phenotypic differences on a genomic level, we examined indices of amino acid substitutions that have previously been linked with cold adaptation via an increase in protein flexibility. We found that these indices were significantly correlated with OGT at the whole genome level, although the sign of some correlations were opposite of the predicted positive correlation between temperature and the indices. Using these data, we fit a multiple linear regression model for OGT within the Colwelliaceae family that incorporates the three most informative amino acid indices: GRAVY, Aliphatic Index, and Acidic Residue Proportion. Additionally, a putative cold-adaptive gene cassette was identified that was likely introduced by horizontal gene transfer between two closely related clades with different OGTs. These contributions offer key insights into OGT variability and its underlying genomic foundation in the family Colwelliaceae.

A Preliminary Analysis Of How A Software Organization’S Maturity And Size Affect Its Intellectual Property Portfolio, Daniel Gifford

Master of Science in Software Engineering Theses

Intellectual property, commonly known as IP, is complex. The four main types of software IP, which is what this thesis will focus on, are patents, trade secrets, trademarks, and copyright. Patents, trade secrets, and copyrights were all studied by this thesis. Software IP is unique in that it can by copyrighted. Different IP owners, which can be businesses of different types, individuals, and universities, often have different strategies as to how to use their IP portfolio. This thesis studies differences in IP usage between these entities specifically in the field of software. Large and small software companies were analyzed specifically. …

Infection-Induced 5'-Half Molecules Of Trnahisgug Activate Toll-Like Receptor 7., Kamlesh Pawar, Megumi Shigematsu, Soroush Sharbati, Yohei Kirino

Computational Medicine Center Faculty Papers

Toll-like receptors (TLRs) play a crucial role in the innate immune response. Although endosomal TLR7 recognizes single-stranded RNAs, their endogenous RNA ligands have not been fully explored. Here, we report 5'-tRNA half molecules as abundant activators of TLR7. Mycobacterial infection and accompanying surface TLR activation up-regulate the expression of 5'-tRNA half molecules in human monocyte-derived macrophages (HMDMs). The abundant accumulation of 5'-tRNA halves also occur in HMDM-secreted extracellular vehicles (EVs); the abundance of EV-5'-tRNAHisGUG half molecules is >200-fold higher than that of the most abundant EV-microRNA (miRNA). Sequence identification of the 5'-tRNA halves using cP-RNA-seq revealed abundant and selective packaging …

Quantifying Deepfake Detection Accuracy For A Variety Of Natural Settings, Pratikkumar Prajapati

Master's Projects

Deep fakes are videos generated from a starting video of a person where that person's face has been swapped for someone else's. In this report, we describe our work to develop general, deep learning-based models to classify Deep Fake content. Our first experiments involved simple Convolution Neural Network (CNN)-based models where we varied how individual frames from the source video were passed to the CNN. These simple models tended to give low accuracy scores for discriminating fake versus non-fake videos of less than 60%. We then developed three more sophisticated models: one based on choosing test frames, one based on …

State Of Idaho V. Rachel Elizabeth Luna Appellant's Brief Dckt. 47760

Not Reported

No abstract provided.

State V. Ybarra Appellant's Brief Dckt. 48016

Not Reported

No abstract provided.

State V. Scruggs Respondent's Brief Dckt. 48020

Not Reported

No abstract provided.

State V. Suckow Appellant's Brief Dckt. 48047

Not Reported

No abstract provided.

State V. Clapp Appellant's Brief Dckt. 48049

Not Reported

No abstract provided.

State V. Lake Respondent's Brief Dckt. 48057

Not Reported

No abstract provided.

State V. Mallak Respondent's Brief Dckt. 48169

Not Reported

No abstract provided.

Pretrial Release Policy Portfolios: Examining Changes Over Time In States With Low Policy Activity, Madison Kristine Doyle

Theses and Dissertations

Changes in the policy process is one of the lesser explored phenomena within the field of criminal justice. A basis of knowledge about changes in various criminal justice policy processes in the U.S., could benefit scholars and practitioners alike. This thesis explores changes in content of pretrial release policy (PRP) portfolios in states with the least policy activity both within and across states over time. A thematic content analysis of PRPs from eight U.S. states (Alaska, Arkansas, Idaho, Maine, North Dakota, South Dakota, Vermont, and Wyoming) revealed how PRPs have changed in those states over time. States that had fewer …

The Effects Of Exercise On Breastmilk Composition And Supply: A Critical Literature Review, Jewell Mcroy

Masters Theses, 2020-current

It is well established that breastfeeding provides dual benefits to both the mother and the infant and is the preferred method of providing nutrients to infants essential for growth and development. Participating in regular physical exercise is also well-supported by research to be beneficial to one’s overall health and quality of life. Currently there are no physical activity guidelines developed for women who are postpartum and breastfeeding. An online database search was conducted; three articles met the inclusion criteria and were included within the review. Studies were included if there were direct measures of human breastmilk composition, supply/volume, and included …

Analytical And Clinical Sample Performance Characteristics Of The Onclarity Assay For The Detection Of Human Papillomavirus, Stephen Young, Laurence Vaughan, Karen Yanson, Karen Eckert, Aojun Li, James Harris, Aaron Ermel, James A. Williams, Mohammad Al-Ghoul, Catherine L. Cammarata, Stephanie N. Taylor, Ronald Luff, Charles K. Cooper, Barbara Van Der Pol

Pathology Research and Scholarship

The objective of this study was to determine the result reproducibility and performance of the BD Onclarity human papillomavirus (HPV) assay (Onclarity) on the BD Viper LT platform using both contrived and clinical specimens. Reproducibility was assessed in BD SurePath liquid-based cytology (LBC) medium (SurePath) using contrived panels (HPV genotype 16 [HPV16] positive, HPV18 positive, or HPV45 positive) or clinical specimens (HPV16, -18, -31, -33/58, -45, or -52 positive or HPV negative). In addition, specimens from 3,879 individuals from the Onclarity trial were aliquoted prior to or following cytology processing and tested for HPV. Finally, specimens were collected using either …

Growth Strategies Through Mergers And Acquisitions In Knowledge-Based Companies – The Case For Architect/Engineer (A/E) Professional Service Firms (Psfs), Terry A. Ruhl

College of Business Theses and Dissertations

Analyses of mergers and acquisitions (M&A) across industries are prevalent in the literature. Yet, this research reveals that at least half, if not more, of all M&A activity fails to meet strategic, operational, and financial objectives, and can result in declining employee and customer satisfaction. The research herein develops a model framework for alternative growth strategies in the architecture/engineering (A/E) industry, with a specific focus on the antecedents of successful M&A among A/E professional service firms (PSFs).At the center of the model is the Resource Based View of the Firm, where leadership capacity, cash, and capital flows yield an affordable …

Brian Reynolds, Public Visibility, And Gay Stardom, Finley Freibert

Faculty and Staff Scholarship

Once gracing the covers of numerous gay newspapers and magazines, Brian Reynolds was a key figure of Los Angeles’ emergent gay adult film industry of the late 1960s. He had all but disappeared from gay adult film historiography until he re-emerged as a cover model for a scholarly journal in 2012, to illustrate pioneering scholarship that initiated contemporary Pat Rocco studies. This article puts the story of Brian Reynolds in dialogue with critical star studies in order to offer a recovery history of Reynolds. Reynolds’ rise to celebrity and sudden relegation to obscurity underscores the historical instability of gay pornographic …

Exploring Patient And Staff Experiences Of Video Consultations During Covid-19 In An English Outpatient Care Setting: Secondary Data Analysis Of Routinely Collected Feedback Data, Hl Bradwell, Rl Baines, Kj Edwards, Sg Stevens, K Atkinson, E Wilkinson, A Chatterjee, Rb Jones

School of Nursing and Midwifery

No abstract provided.

Large Animal Genetic Engineering Summit 2020, Irina A. Polejaeva

Funded Research Records

No abstract provided.

Technoference: Parent Mobile Device Use And Implications For Children And Parent-Child Relationships., Brandon T. Mcdaniel Phd

Health Services and Informatics Research

The increase in the prevalence of smartphones and mobile devices has spurred changes in the caregiving environment of infants and young children, as phones and mobile devices are used at times during caregiving and in caregiving spaces. This use could create disruptions and cause distractions during parenting (termed technoference). This article summarizes the potential impacts on parent responsiveness and the experiences of infants and young children. Yet, it also warns that it is important to consider the reason for and type of parent use. Finally, the article ends with practical tips for working with parents concerning mobile device use.

Does Early Surgery Prevent Postoperative Icu Admission After Surgery For The Fracture Of The Hip. Nested Case Control Study Of 911 Patients, Obada Hasan, Laraib Mazhar, Umar Rabbani, Amna Rabbani, Fatima Mahmood, Shahryar Noordin

Department of Medicine

Introduction: Since most hip fractures are treated surgically, it is imperative to find an optimum fracture-to-surgery time to decrease the potential complications and enhance postoperative outcomes. In comparison to the vast plethora of literature available on surgical delay and its implications on mortality, very little, if any, research is available on the impact of delayed surgery on postoperative ICU admission. The primary objective of our study is to examine the factors influencing post-surgical ICU admission in order to work on preventive strategies to reduce the potential associated morbidity.
Material and methods: Investigators did a nested case control study in a …

The Reproductive Biology Of Yelloweye Rockfish (Sebastes Ruberrimus) In Prince William Sound And The Northern Gulf Of Alaska, Donald E. Arthur

Fisheries

Over the last half century, Yelloweye Rockfish Sebastes ruberrimus have experienced dramatic declines along the West Coast of the United States and British Columbia. Efforts have been made throughout the species' range to rebuild and sustainably manage stocks, including the introduction of a Statewide Rockfish Initiative by the State of Alaska, which intends to develop management strategies for Yelloweye Rockfish in the Gulf of Alaska. To support this effort and address information gaps in Yelloweye Rockfish reproductive biology throughout their range, I estimated important reproductive parameters and life history information for Yelloweye Rockfish in Prince William Sound and the Northern …

Midweek Memo, Georgia Southern University

Midweek Memo

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Roads And Airfields Constructed On Permafrost: A Synthesis Of Practice, Billy Connor, Douglas J. Goering, Mikhail Kanevskiy, Erin Trochim, Kevin L. Bjella, Robert L. Mchattie

AIDC Project Reports

This synthesis provides the practicing engineer with the basic knowledge required to build roadway and airports over permafrost terrain. Topic covered include an overview of permafrost, geotechnical investigations, slope stability, impacts of climate, and adaptation strategies during the design, construction and maintenance phases. The purpose of the synthesis is not to provide a comprehensive body of knowledge or to provide a complete how‐to manual. Rather the synthesis provides a working knowledge for those working in permafrost regions such that the practicing engineer will be able to work with subject matter experts to obtain the desired project outcomes.

The George-Anne Daily, Georgia Southern University

George-Anne Media Group: Newsletters & Magazines

No abstract provided.

Scattering Of Goldstone Bosons And Resonance Production In A Composite Higgs Model On The Lattice, V Drach, T Janowski, C Pica, S Prelovsek

School of Engineering, Computing and Mathematics

No abstract provided.

Palestinian Women’S Agency, Yasmin Khodary

Political Science

In Civil Resistance and Conflict Transformation, Veronique Dudouet divides responses to oppression into two main analytical categories. The first category refers to conventional actions, including party politics, advocacy or diplomacy, dialogue and negotiations, and litigation. The second category is non-intuitional/contentious actions including: first, nonviolent/unarmed/peaceful resistance through protest and persuasion, non-cooperation, disruptive intervention and creative resistance and second, violent/armed resistance through warfare, terrorist attacks, guerrilla insurgency, protest violence and sabotage. These two sub-categories of resistance operate outside the bounds of conventional political channels and are usually employed by an oppressed minority or disempowered majority by an exclusionary national or foreign actor. …

Root Uptake Under Mismatched Distributions Of Water And Nutrients In The Root Zone, Jing Yan, Nathaniel A. Bogie, Teamrat A. Ghezzehei

Faculty Research, Scholarly, and Creative Activity

Most plants derive their water and nutrient needs from soils where the resources are often scarce, patchy, and ephemeral. It is not uncommon for plant roots to encounter mismatched patches of water-rich and nutrient-rich regions in natural environments. Such an uneven distribution of resources necessitates plant reliance on strategies for exploring and acquiring nutrients from relatively dry patches. We conducted a laboratory study that elucidates the biophysical mechanisms that enable this adaptation. The roots of tomato (Solanum lycopersicum) seedlings were laterally split and grown in two adjacent, hydraulically disconnected pots, which permitted precise control of water and nutrient applications to …

Feasibility Of Multiple Camera Large-Scale Particle Image Velocimetry Techniques For Rivers In Alaska, Eric N. Lamesjerant

Engineering

Alaska is characterized by sparse hydrologic data. Non-intrusive gauging is one method of increasing the data available but is limited in its current application. This study seeks to assess the feasibility of using commercially available software and multiple cameras to diversify the conditions for which large-scale particle image velocimetry may be applied. Using available software and the deployment of multiple cameras, stereoscopically determined discharge is compared with discharge determined using an acoustic Doppler current profiler and accepted single camera practices currently in use with large-scale particle image velocimetry. The results indicate that the use of commercial software and multiple cameras …

Scholarly Communications In Knowledge Economy By A Premier Indian Chemical Sciences R&D Laboratory - Csir-Ncl : A Scientometric Evaluation, Gati Krushna Nayak, Sudam Charan Sahu, Vaishali S. Khaparde, Sudeshna Tripathi

Library Philosophy and Practice (e-journal)

This paper made an attempt to analyze publications of CSIR-NCL, Pune a reputed world class 70 years renowned Chemistry & Chemical Engineering R&D Laboratory during the last 10 years w.e.f. 2010-2019 as reflected in SCOPUS multidisciplinary international database. The total number of 5372 research publications have been downloaded and analyzed according to the objectives. This paper gives a complete view of the evaluation of research outcomes of the CSIR-NCL, Pune the productive and influential in innovative research. This paper vigorously tries to give a complete view of the evaluation of research outcomes of the CSIR-NCL, Pune in India that ought …

Regional Trends In Birth Weight In Low- And Middle-Income Countries 2013-2018, Irene Marete, Osayame Ekhaguere, Carla M. Bann, Sherri L. Bucher, Paul Nyongesa, Archana B. Patel, Patricia L. Hibberd, Sarah Saleem, Robert L. Goldenberg, Shivaprasad S. Goudar

Community Health Sciences

Background: Birth weight (BW) is a strong predictor of neonatal outcomes. The purpose of this study was to compare BWs between global regions (south Asia, sub-Saharan Africa, Central America) prospectively and to determine if trends exist in BW over time using the population-based maternal and newborn registry (MNHR) of the Global Network for Women'sand Children's Health Research (Global Network).
Methods: The MNHR is a prospective observational population-based registryof six research sites participating in the Global Network (2013-2018), within five low- and middle-income countries (Kenya, Zambia, India, Pakistan, and Guatemala) in threeglobal regions (sub-Saharan Af rica, south Asia, Central America). The …