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Consumptive Coagulopathy Of Severe Yellow Fever Occurs Independently Of Hepatocellular Tropism And Massive Hepatic Injury, Adam L. Bailey, Liang-I Kang, Luiz Gonzaga Francisco De Assis Barros D'Elia Zanella, Cássia G. T. Silveira, Yeh-Li Ho, Lander Foquet, Greg Bial, Broc T. Mccune, Amaro Nunes Duarte-Neto, Archana Thomas, Hans-Peter Raué, Kathleen Byrnes, Esper G. Kallas, Mark K. Slifka, Michael S. Diamond

Open Access Publications

No abstract provided.

Prevalence Of Cryptococcal Antigen And Outcomes In People With Human Immunodeficiency Virus In Honduras: A Cohort Study, Julio C. Zuniga-Moya, Jane A. O'Halloran, Andrej Spec, Et Al

Open Access Publications

Background: Cryptococcal meningitis is a major cause of death among people with human immunodeficiency virus (PWH). Cryptococcal antigen (CrAg) testing of asymptomatic patients is an important public health measure to reduce mortality in high-incidence areas. However, limited data exist on CrAg prevalence in Central America.

Methods: We conducted a prospective cohort study at the 2 largest human immunodeficiency virus (HIV) clinics and hospitals in Honduras. Cryptococcal antigen in serum and cerebrospinal fluid was performed in individuals with HIV who had CD4 ≤100 cells/mm

Results: A total of 220 PWH were tested for CrAg, 12.7% (n = 28) of which tested …

Analysis Of Changes In Genes That Lead To Antibiotic Resistance In Klebsiella Pneumoniae 43816, Jasmine R. Anderson

UNF Graduate Theses and Dissertations

Klebsiella pneumoniae is a Gram-negative bacterium responsible for nosocomial infections such as blood stream infections, meningitis, and septicemia. Clinical isolates confirm K. pneumoniae is increasingly antibiotic resistant (AR) making treatment more difficult. While bacterial exposure to antimicrobial substances is a known cause of the AR phenotype, there still exists gaps in knowledge about the genetic changes responsible for this fitness change. The laboratory Klebsiella pneumoniae 43816 (ATCC 43816) was exposed to sublethal concentrations of Cephalothin for a 14-day period. At the end of the experimental treatment, the adapted population acquired clinical antibiotic resistance along with phenotypic and morphological changes. The …

Be A Hero Too, Samantha S. Rohe, Sophie Cemaj

Books: Pediatrics

This children's book explains COVID-19 and how to decrease the incidence of transmissions.

Toxin Secretion By Mycobacterium Tuberculosis, Uday Tak

All ETDs from UAB

Mycobacterium tuberculosis (Mtb) is a highly infectious airborne bacterial pathogen, and the causative agent of human tuberculosis. Mtb infects approximately one-third of the human population, and is responsible for more deaths than any other infectious disease in the history of mankind. Mtb is an intracellular pathogen and primarily infects myeloid cells, such as macrophages in the human lung. In macrophages, Mtb establishes a replication niche within membrane-bound vacuoles named phagosomes, while subverting immunity. The intracellular survival of Mtb depends on manipulation of host cellular processes through secreted effector proteins. Ultimately, this intracellular vacuole is ruptured by the type VII secretion …

Understanding The Impact Of Diet And Obesity On Anti-Tumor Immunity And Therapeutic Efficacy In Kidney Cancer, Rachael Miller Orlandella

All ETDs from UAB

Obesity is regarded as a major risk factor for developing renal cell carcinoma (RCC). Advanced-stage RCC exhibits chemotherapeutic resistance, but is responsive to immunotherapies such as the immune checkpoint inhibitor anti-programmed cell death-1 (anti-PD-1). Despite some clinical successes, response rates remain low for anti-PD-1 monotherapy (20-30%). Preclinical evaluations of immunotherapeutic strategies typically use lean mice and do not account for patient comorbidities. This may explain the underwhelming success rates following clinical translation of new cancer treatments. Recent studies found that obesity was associated with favorable outcomes and responses to immunotherapy in melanoma. However, the effects of obesity on anti-tumor immunity …

Novel Hiv Adaptation Strategy Against Host Cd8 T Cell Immunity, Kai Qin

All ETDs from UAB

Since the 1980s, HIV infection has caused millions of death and tremendous economic losses worldwide. Despite great scientific advances, the only effective therapy to date, antiretroviral therapy (ART), merely delays disease progression yet cannot eradicate viral infection. Therefore, an effective HIV vaccine that can prevent the acquisition of infection would be a desirable alternative. The critical role of CD8 T-cell immunity in HIV infection has been well documented and therefore not surprisingly a great deal of effort has been focused on developing an effective CD8 T-cell based HIV vaccine. However, by far, such CD8 T-cell based HIV vaccine trials have …

Protective Host Responses During Acute And Chronic Lung Exposure To Aspergillus Fumigatus, Joseph Mackel

All ETDs from UAB

Aspergillus fumigatus is an environmental airborne fungus that acts as both an opportunistic pathogen and an allergen. In immunocompromised hosts, A. fumigatus can germinate in the lung, cause tissue damage and pneumonia, and disseminate to other organs, causing a disease termed invasive aspergillosis (IA). In severe asthma, sensitization to fungi such as A. fumigatus is associated with more severe disease. Determination of host and fungal factors that drive immunoprotection versus immunopathogenesis in both of these settings will ultimately enable therapeutic modulation of immune responses to improve disease outcomes. In this body of work, we build on recent findings that implicate …

Inspire : Vol 3, Issue 1, Department Of Medicine

INSPIRE – Department of Medicine Archives

Content

• Message From the Chair
• Global Fund TB Audit-Reviewing diagnostic algorithms used for clinical diagnosis of TB
• Student Research Forum’s 5th Journal Club
• Medicine at National and International Forums
• Featured Faculty: Ms Safia Awan “Don’t ever give up on your dreams.”
• What are Academics Around the World Reading This Month?
• Publications

Copper Dependent Inhibitors Subvert Common Resistance Mechanisms In Staphylococcus Aureus And Inhibit Crucial Metabolic Pathways, Cameron Crawford

All ETDs from UAB

The effective antibiotic pool is shrinking with antibiotic resistance a constant problem that threatens to make our current therapies obsolete. To combat this, new drug screening methodologies are required since the traditional drug screens are offering limited results or only improvements upon current treatments. This work expands upon the idea that transition metal toxicity can be preferentially targeted towards bacteria. Specifically, the focus is on copper dependent inhibitors that quickly work to eliminate Staphylococcus aureus. S. aureus is a particularly threatening bacteria with high strain variability, a litany of survival and virulence factors, and multiple drug resistance mechanisms that are …

Legislating A Negative Right To Health: Health Impact Assessments, Christina S. Ho

Seton Hall Law Review

No abstract provided.

Factors Associated With Hiv Testing In Liberia: Findings From The 2013 Demographic Health Survey, Miatta M.E. Dennis

College of Graduate Studies: Theses & Dissertations

Human immunodeficiency virus (HIV) is among the world’s most serious challenges. The African region is severely affected with nearly one in 25 adults being HIV infected –accounting for over two-thirds of HIV infected people worldwide. In 2013, approximately three in four men and half of women aged 15-49 had never been HIV tested. In 2018, over 7,000 Liberians were HIV positive. Increasing HIV testing rates is the primary step to reducing HIV infection rates. This study examines factors associated with HIV testing behavior among Liberians using the Knowledge Attitude Behavior (KAB) model as a conceptual framework. The 2013 Liberia Demographic …

Elucidating The Regulatory Mechanisms For The Cholesterol Catabolic Pathway Transcriptional Repressor Kstr Of Mycobacterium Tuberculosis, Chenoa D. Arico

Open Access Theses & Dissertations

Mycobacterium tuberculosis (Mtb) is a bacterium that has been able to establish itself throughout evolution and is currently still an ever present threat resulting in approximately 2 million deaths per year worldwide. The ability for Mtb to utilize cholesterol as a carbon source is maintained through transcriptional regulation mechanisms not fully understood. The Rv3574 gene codes for the self-regulated TetR-like transcriptional repressor KstR that controls the expression of large clusters of cholesterol catabolic genes in Mtb. This study aims to provide insights on the regulatory mechanisms of cholesterol catabolism, which could be used as therapeutic target to treat and control …

Factors Contributing To Community-Associated Carbapenem Resistant Enterobacteriaceae Infections In The United States, Zabrina Lockett

Walden Dissertations and Doctoral Studies

Carbapenem-resistant Enterobacteriaceae (CRE) health care acquired infections are a serious public health threat due to a high mortality rate, economic burden, and depletion of last resort CRE antimicrobials. CRE infections have emerged as community-associated type infections in the United States. For CRE to now have that type of potential is cause for immediate and extensive action. The prevalence of CRE infection cases that were reported as community-associated CRE and health care acquired CRE by examining specific clinical characteristics between 4 selected states that were reported to respective state health departments and the Centers for Disease Control were explored in this …

Experiences Of Refugee Women With Different Physical Abilities In Kenya, Grace Faraja Nkundabantu

Walden Dissertations and Doctoral Studies

Refugee populations worldwide increase every day as a result of social and political wars. Women and children, particularly those with different physical abilities, are the most vulnerable populations affected by atrocities in their home countries and in their countries of refuge. In Africa, it is believed that women with different abilities bring shame to their families. The purpose of this descriptive phenomenological study was to explore the lived experiences of refugee women with different physical abilities, specifically regarding challenges related to accessing services in Kenya. The research question that guided this study focused on the lived experiences of refugee women …

Population Density And Spatial Distribution Of Neglected Tropical Diseases In Sierra Leone, Abou B. Funna

Walden Dissertations and Doctoral Studies

Neglected tropical diseases (NTDs) are the most common health conditions affecting the poorest residents of sub-Saharan Africa (SSA). These infections affect an estimated 2 billion people worldwide, including 500 million people living in SSA. About 85% of NTD infections are a result of helminth infections; hookworm is also a common occurrence among SSA’s poorest people, especially children. Schistosomiasis is the 2nd most prevalent NTD after hookworm. This quantitative correlational study investigated population distribution and the spread of NTDs in Sierra Leone. The focus was on 5 major NTDs: ascariasis, hookworm, schistosomiasis, soil-transmitted helminthiasis, and trichuris. Data were obtained from the …

A Health Care Management Organization's Internal Controls Strategy For Managed Care, Elizabeth E. David

Walden Dissertations and Doctoral Studies

A lack of effective operational internal controls among health management organization (HMO) leaders could lead to poor operational practices, mismanagement of the government's health care funds, increased health care spending, and a negative impact on patient health outcomes. Grounded in transformational leadership theory, the purpose of this qualitative, single case study was to explore successful internal control strategies used by leaders at a southeastern Pennsylvania HMO. Data were collected from semistructured interviews with 5 participants who held positions of director or above within the organization for at least 3 years, had institutional knowledge, and had responsibility for overseeing the organization's …

Improving Rabies Aftercare By Educating Emergency Department Providers Of Care, Gloria Denise Evans

Walden Dissertations and Doctoral Studies

According to an evaluation completed by the local health department in the state where the project was conducted, patients were not returning to the emergency department (ED) for post-exposure prophylaxis (PEP) rabies vaccine as recommended. Educating the ED providers on care, who are usually the first point of contact after an animal bite with possible rabies virus transmission, is critical to improving patients' outcomes secondary to rabies postexposure. The focused for this project is educating ED personnel about rabies PEP administration after rabies virus exposure which will their increase knowledge regarding rabies follow-up care. Lewin's change theory provided the theoretical …

Modulation Of Oral Microbiota: A New Frontier In Exercise Supplementation, Raul Bescos, Zoe L.S. Brookes, Louise A. Belfield, Manuel Fernandez-Sanjurjo, Patricia Casas-Agustench

Peninsula Dental School

No abstract provided.

Covid-19: An Update From England’S High Consequence Infectious Diseases Intensive Care Unit Leads, Daniel Martin, Sarah Platt, Peter Hampshire, Chris Meadows

Peninsula Medical School

Each winter, the population and healthcare services around the globe brace themselves for what is usually a fairly unpredictable bombardment by a myriad of microbial pathogens. From an immunological perspective, human hosts are constantly modifying our defences, aiming to reduce the potential impact of this invisible force. We can bolster the fight by using annual vaccinations against influenza but by definition, we must already have an intimate knowledge of our enemy to generate this additional immunological shield. Sadly, newcomers on the microbial scene tend to slip straight through net and hit us hard. Hence the effectiveness of the seasonal flu …

Public Health Law Tools: A Brief Guide, Kristen Underhill

Faculty Scholarship

The COVID-19 pandemic offers a threat with few precedents in modern times and tests the tools of modern surveillance and public health law. The goal of this chapter is to provide a brief overview of the types of measures that state and federal governments can invoke to treat and prevent the spread of infectious disease. The following sections will provide an orientation to domestic public health law followed by the types of measures available to state and federal public health authorities.

Although this chapter does not consider international institutions, treaties, or norms that affect public health, these are also essential …

Linked Fate: Justice And The Criminal Legal System During The Covid-19 Pandemic, Susan P. Sturm, Faiz Pirani, Hyun Kim, Natalie Behr, Zachary D. Hardwick

Faculty Scholarship

The concept of “linked fate” has taken on new meaning in the face of the COVID-19 pandemic. People all over the world – from every walk of life, spanning class, race, gender, and nationality – face a potentially deadly threat requiring cooperation and sacrifice. The plight of the most vulnerable among us affects the capacity of the larger community to cope with, recover, and learn from COVID-19’s devastating impact. COVID-19 makes visible and urgent the need to embrace our linked fate, “develop a sense of commonality and shared circumstances,” and unstick dysfunctional and inequitable political and legal systems.

Nowhere is …

Suicidal Risk Education In Hospice Care, Shannon Jones

Doctor of Nursing Practice Projects

It is well-documented that oncology patients experience greater rates of suicidality including suicidal ideation, actions, or completions, as compared to the general population. The small, rural county which was the setting for this project has a higher rate of lung and colon cancers as compared to regional, state, and national rates. A review of the literature revealed best practices for suicide screening including universal education and screening and targeted education and screening for at-risk populations. The rate for suicides in the county is also higher than the rates for the region, state, and nation. The purpose of this project was …

Association Of A Repetitive Manual Task On Inflammatory Biomarker Expression, Heart Rate Variability And Rating Of Perceived Exertion, Daniel Conde

Open Access Theses & Dissertations

The National Institute for Occupational Safety and Health (NIOSH) defines work related musculoskeletal disorders (WMSDs) as a group of disorders involving muscles, tendons, and nerves. The WMSDs symptoms are considered the most common occupational disease, causing limitations in daily activities. Even though there is an association between repetitive manual tasks involving short work cycles, force demanding tasks, and the development of WMSDs, the specific cause of the physiological changes over a long period of time is a cause of debate. The study of mechanisms underlying the physiological changes is essential for the early detection, diagnosis, and appropriate management of WMSDs.

Identification Of A Novel Single Amino Acid Substitution (V666g) Of Jak1 From A Patient With Acute Lymphoblastic Leukemia Impairs Jak3 Mediated Il-2 Signaling, Alice Hernandez Grant

Open Access Theses & Dissertations

The Janus kinase (JAK) family, notably JAK1, JAK2 and JAK3 are recognized as oncogenic drivers in high risk Acute Lymphoblastic Leukemia (ALL). The bulk of activating JAK mutations are thought to occur within functional hot-spots across Janus Homology (JH) domains. The most frequently mutated regions is the JH2 pseudo-kinase, which provides a negative regulatory role to the adjacent catalytically active JH1 kinase domain. Despite the prevalence of JAK activating mutations and a need for new therapeutic inhibitors, there is a lack of understanding in the allosteric regulation of JAK kinases. Here we sought to identify mutations involved in driving ALL …

Isolation And Characterization Of Α-Gal-Containing Extracellular Vesicles (Evs) From Three Major Genotypes Of Trypanosoma Cruzi: Potential Biomarkers Of Chagas Disease, Nasim Karimi Hosseini

Open Access Theses & Dissertations

Chagas disease (ChD) is a neglected tropical disease (NTD) caused by the protozoan parasite, Trypanosoma cruzi. It is transmitted by the insect-vector triatomine (popular known as kissing bug), blood transfusion, organ transplantation, congenitally, and contaminated foods and juices. T. cruzi has evolved several strategies to invade the host cells, including the release extracellular vesicles (EVs), which assist pathogen survival and its replication within the host. T. cruzi is covered with highly glycosylated surface molecules such as glycoproteins and glycolipids, which are shown to be involved in the interaction with host immune cells. These molecules are highly immunogenic and reactive with …

Expression And Functional Sialome Of Triatomines, Insect Vectors Of Chagas Disease, Maria Tays Mendes

Open Access Theses & Dissertations

Triatomines are blood-sucking arthropods that transmit Trypanosoma cruzi, the etiologic agent of Chagas disease (ChD). Triatomines use bioactive molecules in the saliva for successful blood feeding and to evade the hemostatic and immune defense system of the hosts. Knowing the saliva composition could be useful for a better understanding which and how insect-derived molecules might influence host-parasite interactions. Previous studies have shown that some saliva-derived proteins and lipids can modulate the host immune system and increase T. cruzi infection. We hypothesize that the triatomine saliva contains a great diversity of lipids and proteins that can modulate the mammalian host immune …

Molecular And Functional Characterization Of Α-Galactosyl Epitopes In Trypanosoma Cruzi, Uriel Ortega Rodriguez

Open Access Theses & Dissertations

Trypanosoma cruzi, the causative agent of Chagas disease (CD) currently affects 6-7 million people across the world. Currently, only two drugs, benznidazole and nifurtimox, are available for treatment of CD and they are highly toxic and less effective in the chronic stage of the disease. Specific biomarkers for diagnosis and follow-up of treatment do not exist in the clinical settings. following chemotherapy, patients take approximately 10-20 years to exhibit negative seroconversion with the conventional serology assays. Moreover, there is no vaccine available to prevent or treat CD. T. cruzi contains a complex cell surface consisting of several classes of glycoconjugates …

Identification Of Interkingdom Interactions Of Cryptococcus Neoformans In Pigeon Excreta Using Next Generation Sequencing, Marlynn Cadena

Open Access Theses & Dissertations

Cryptococcus neoformans is an encapsulated fungus with worldwide distribution and associated with pigeon excreta. C. neoformans causes pulmonary cryptococcosis in immunocompromised populations, which results in high mortality. C. neoformans main virulence factor is its polysaccharide capsule that confers the fungus with the ability to form biofilms. Interkingdom interactions can have a positive, neutral or negative effect on C. neoformans in relation to its virulence and/or pathogenicity. C. neoformans survival within polymicrobial and other challenging communities has been shown to affect the evolution of its virulence factors. For example, the size of C. neoformans polysaccharide capsule is modulated during interactions with …

Discovery Of Glycan-Based Biomarkers For Cutaneous Leishmaniasis And Chagas Disease By Reversed Immunoglycomics, Alba Lucia Montoya

Open Access Theses & Dissertations

Protozoa are the causative agents of a number of diseases that affect humans and mammalian animals. Several forms of leishmaniasis are caused by different Leishmania species (Leishmania spp.), while Chagas disease (CD) is caused by the parasite Trypanosoma cruzi (T. cruzi). These members of the Trypanosomatidae family have characteristic glycoconjugates broadly distributed on their cell surfaces, which can be useful for diagnosis and follow-up of chemotherapy for the diseases.

Leishmania spp. expresses an “exotic” surface glycocalyx mainly composed of a number of glycosyl-phosphatidylinositol (GPI)-anchored proteins, a complex lipophosphoglycan (LPG) and a family of low-molecular mass glycoinositol-phospholipids (GIPLs), some of them …