Digital Commons Network^™

Blend It Like Beckett: Samuel Beckett And Experimental Contemporary Creative Writing, Sam Nicole Campbell

Electronic Theses and Dissertations

Samuel Beckett penned novels, short stories, poetry, stage plays, radio plays, and scripts—and he did each in a way that blended genre, challenged the norms of creative writing, and surprised audiences around the globe. His experimental approach to creative writing included the use of absurdism, genre-hybridization, and ergodicism, which led to Beckett fundamentally changing the approach to creative writing. His aesthetics have trickled down through the years and can be seen in contemporary works, including Aimee Bender’s short story collection The Girl in the Flammable Skirt and Mark Z. Danielewski’s novel House of Leaves[1]. By examining these works …

Biophysical Characterization Of Protein-Nanoparticle Interactions, Yasiru Randika Perera

Theses and Dissertations

Nanoparticles (NPs) have become a key tool in medicine and biotechnology; as drug delivery systems, biosensors, and diagnostic devices. However, the mechanism of biocorona formation on nanoparticle surfaces and their impact on drug delivery remains speculative. Nevertheless, functionalized nanoparticles have demonstrated major success in medical applications; having been shown to effectively treat disease. The mechanistic details of protein behavior on nanoparticle surfaces remain poorly understood to date; due to difficulty in determining the orientation and structure of protein on NPs. Furthermore, surface crowding, orientation, and degree of disorder have been shown to perturb the efficacy of protein on NPs; dramatically …

Draft Genome Sequences Of Two Extensively Drug-Resistant Strains Of Acinetobacter Baumannii Isolated From Clinical Samples In Pakistan, Sara Lomonaco, Matthew A. Crawford, Christine Lascols, Debra J. Fisher, Kevin Anderson, David R. Hodge, Segaran P. Pillai, Stephen A. Morse, Erum Khan, Molly A. Hughes, Marc W. Allard, Shashi K. Sharma

Department of Pathology and Laboratory Medicine

Infections in immunocompromised patients that are caused by extensively drug-resistant (XDR) Acinetobacter baumannii strains have been increasingly reported worldwide. In particular, carbapenem-resistant A. baumannii strains are a prominent cause of health care-associated infections. Here, we report draft genome assemblies for two clinical XDR A. baumannii isolates obtained from hospitalized patients in Pakistan.

Metagenomic Sequencing, Microbiome Reconstruction, And Analysis Of Ancient North And Central American Dental Calculus, Jonathan Belanich

Theses and Dissertations

Analyses of human oral microbiomes reveal substantial amounts of information about health, diet, and diseases of individuals and their communities within the archaeological record. In order to examine bacterial genomes from the past, specific archaeological samples that contain remnants of the microbial communities in question must be utilized. Recent developments in high-throughput, next-generation DNA sequencing have enabled the characterization of entire oral microbiomes and genomes from the remains of the bacteria trapped in calcified dental plaque. This project analyzed samples of ancient human dental calculus from North and Central America, which were examined for the changes within the oral microbiome …

Physiological Consequences Of Adverse Early-Life Experiences: A Skeletal Investigation Of Frailty And Resilience Within An Institutionalized Sample Using A Modified Version Of The Skeletal Frailty Index (Sfi), Ashley Dafoe

Theses and Dissertations

This study investigates frailty, defined as the accumulation of deficits in physiological functioning, by applying the Skeletal Frailty Index (SFI) to a skeletal sample (N=67) recovered from the Mississippi State Asylum (MSA), and in a comparative sample, the Terry Collection. The SFI was statistically modified to increase its utility here. Variables that influence frailty, including age, sex, stress in early-life, and resilience, were assessed relative to four SFIs: Overall, Nutritional, Activity, and Infection. This study finds that the predicted relationships between the SFIs and the aforementioned variables are largely absent in the MSA sample. When compared to individuals in the …

Preliminary Investigations Into Pharmacodynamic Monitoring Of Cyclosporine In Cats, Harry Cridge

Theses and Dissertations

Existing pharmacokinetic monitoring tools for cyclosporine fail to correlate with clinical response. In dogs, pharmacodynamic monitoring of nuclear factor of activated T cell (NFAT) regulated cytokines is thought to provide a better overall evaluation of the immune response to cyclosporine than blood levels; however, such monitoring tools are not available in cats. In this study, we designed and optimized a protocol for maximal T lymphocyte stimulation in cats. This is the first step in the development of a pharmacodynamic monitoring tool for cyclosporine in cats based on expression of NFAT-regulated cytokines. We also confirmed that cyclosporine has anti-lymphocytic properties in …

Identification Of Genome-Linked Bacillus Bacteriophage Proteins, Katherine Dyer

Honors Program Theses

Small viruses that can infect bacteria, called bacteriophages, can be found in the soil. These viruses are being examined as a potential treatment against bacterial infections when antibiotics are not available or non-effective. Before viruses can be used as a medical treatment, they must be studied extensively. Previous attempts to sequence the terminal end of the genome of a bacteriophage QCM-11 (Quartz-Crystal-Microbalance-11: Q11) resulted in error. It is thought that a covalently bound protein may be attached to Q11’s DNA, blocking sequencing of the genome from the 5’ end. Proteins bound to the DNA of phages is not unheard of, …

Preventing Germs Spreading In Preschool Children, Erika Hernandez

Capstone Projects and Master's Theses

Young children in childcare centers are exposed to germs everyday and are more vulnerable to diseases being in a group care setting. Children from migrant families with little formal education might be more vulnerable because parents may have little knowledge about the importance of hygiene for their children, or they may not have time to reinforce healthy habits due to long working hours. To help address the lack of healthy habits among preschool children aged 3 to 5 years, I am providing a three-day lesson about how germs are spread and the importance of washing hands to prevent diseases at …

Investigating Attitudes And Beliefs Of University Students Aged 18 To 24 Years That Influence Their Decision To Take Pre-Exposure Prophylaxis Medication, Philip Danquah

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Contemporary research findings affirm that young adults aged 18 to 24 years have high prevalence of new HIV infections. Pre-Exposure Prophylaxis (PrEP) medication is the latest strategy in HIV prevention. Rates of PrEP uptake or use have been negligible among university students aged 18 to 24 years, especially among racial and ethnic minority students.

Objective: The objective of this study is to investigate the attitudes and beliefs that influence the uptake of PrEP medications and the HIV risk behaviors associated with the willingness to take PrEP medications among university students aged 18 to 24 years attending the University of …

The Implementation Of A Respiratory Syncytial Virus Prevention And Awareness Pamphlet In The Daycare Setting, Kaylie Marie Humphreys

UNLV Theses, Dissertations, Professional Papers, and Capstones

Respiratory Syncytial Virus (RSV) is the number one cause of bronchiolitis in children under five years of age and regretfully the number one cause of hospitalization in the first year of life. RSV leads to over three million hospitalizations, two million outpatient visits, and 200,000 deaths in the pediatric population globally each year. While no vaccine is currently available to prevent the spread of RSV, efforts must be shown in spreading awareness of strategies utilized in infection prevention such as hand hygiene and properly covering a cough or sneeze.

This project aimed to present this education to a target population …

Targeting Natural Killer Cells For Improved Immunity And Control Of The Adaptive Immune Response., Stephen Pierce, Eric S. Geanes, Todd Bradley

Manuscripts, Articles, Book Chapters and Other Papers

Natural killer (NK) cells are critical for targeting and killing tumor, virus-infected and stressed cells as a member of the innate immune system. Recently, NK cells have also emerged as key regulators of adaptive immunity and have become a prominent therapeutic target for cancer immunotherapy and infection control. NK cells display a diverse array of phenotypes and function. Determining how NK cells develop and are regulated is critical for understanding their role in both innate and adaptive immunity. In this review we discuss current research approaches into NK cell adaptive immunity and how these cells are being harnessed for improving …

Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey

Master's Theses

Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti …

Barriers To Health: Understanding The Barriers Faced By Community Intervention Projects, Vera Landrum

Master's Theses

Health disparities affect significant portions of the population and are most often experienced by marginalized communities (Wilkin, 2013). Health disparities are also impacted by geographical location, and hunger often affects rural areas (Dutta, Anaele, & Jones, 2013; Rural Health Information Hub, 2017). This study aims to understand how nonprofit organizations focused on addressing food insecurity disseminate information to members of the local community and how the input of community members impacted the overall food insecurity campaign. This study was conducted by interviewing five nonprofit directors and conducting three focus groups with clients of the. aforementioned organizations. The author found that …

Characterizing Erad And Antioxidant Response In Ixodes Scapularis Ise6 Cells During Borrelia Infection, Latoyia Downs

Master's Theses

Ticks are hematophagous ectoparasites that transmit a multitude of diseases to humans. Borrelia burgdorferi (BB) and Borrelia miyamotoi (BM) are both tick-borne pathogens that cause disease in humans and are transmitted by the black-legged tick (Ixodes scapularis). A byproduct of blood digestion generates reactive oxygen species that are toxic and cause oxidative stress which promotes cellular damage and dysfunction. The endoplasmic reticulum (ER) is especially affected by oxidative stress, resulting in a buildup of improperly folded proteins in the ER lumen called ER stress. To prevent cellular damage, the tick utilizes an antioxidant system to neutralize ROS and mechanisms …

From Fighting Wasting To Shedding Excess: An Explanatory Sequential Mixed-Method Study Of Obesity Among People Living With Hiv In Nigeria, John Olajide Olawepo

UNLV Theses, Dissertations, Professional Papers, and Capstones

The human immunodeficiency virus (HIV) infection is a global epidemic, with initial high mortality rates since the first diagnosis over three and a half decades ago. However, the use of antiretroviral therapy (ART) has led to longer, healthier lives for many people living with HIV (PLHIV). Accordingly, aging comes with associated co-morbidities like obesity, diabetes, and cardiovascular diseases. The purpose of this mixed-methods study was to further understand the emergent phenomenon of obesity and overweight among PLHIV who are on treatment with ART, using Nigeria as a case study.

An explanatory sequential mixed-method design was used that involved the analysis …

Tales Of Thriving: Identifying The Underpinnings Of Women's Success In Stem Careers, Sarah E. Thoman

UNLV Theses, Dissertations, Professional Papers, and Capstones

Women remain underrepresented in U.S. science, technology, engineering, and math (STEM) fields. Prior research enumerates many challenges that women experience in STEM and suggests interventions that enhance women’s exposure to STEM, technical acumen in STEM, or offer STEM community. Identifying what enables women to thrive in STEM is a novel approach to extending these efforts using a strengths-based approach. I thus conducted a case study focused on understanding the experiences of successful women in the STEM workforce. Findings revealed three core characteristics to women’s STEM success: synergizing self and science, science career advancement and mastery, and being a STEM change …

Confusion, Respiratory Rate, Shock Index (Crsi-65) Score In The Emergency Department Triage May Be A New Severity Scoring Method For Community-Acquired Pneumonia, Beliz O. Tekten, Tugba A. Temrel, Serkan Sahin

Saudi Medical Journal

Objectives: To investigate whether confusion, respiratory rate, shock index-age ≥65 years (CRSI-65) score, consisting of basic physiological parameters, can be used for severity prediction in patients with community-acquired pneumonia. Methods: This is a prospective cohort and single-center study conducted in Bolu Abant Izzet Baysal University Hospital, Bolu, Turkey between January 2018 and June 2018. The study investigated CRSI-65 score in predicting 4-week mortality and the need for intensive care for patients with community-acquired pneumonia. Results: A total of 58 patients with community-acquired pneumonia admitted to the emergency department were included in this study. Of the patients, 62.1% were males (n=36), …

Blood Culture Contamination In A Tertiary Care Hospital Of Saudi Arabia: A One-Year Study, Hassan A. Hemeg, Abdullah Z. Almutairi, Nujud L. Alharbi, Reema F. Alenezi, Mohammad A. Alturkostani, Hani A. Ozbak, Farhat A. Islam

Saudi Medical Journal

Objectives: To monitor blood culture contamination (BCC) rates in a tertiary care hospital in Saudi Arabia. Methods: Blood cultures submitted to the Microbiology Laboratory of King Fahad Hospital, Madina, Saudi Arabia between January and December 2017 were analyzed prospectively. Positive blood cultures were either designated as true bacteremia with confirmed bloodstream infection or BCC. Results: Among 5,536 blood cultures from 2201 patients, 364 (6.6%) mirrored BCC. There was an upward trend in contamination rates in specific months. With respect to total blood cultures from respective units over a one-year period, medical ward contributed to the highest contamination rate (10.3%). Blood …

New Evidence Published In The Cochrane Library Today Finds Mmr, Mmrv And Mmr+V Vaccines Are Effective And That They Are Not Associated With Increased Risk Of Autism

Saudi Medical Journal

No abstract provided.

Ethics, Ai, Mass Data And Pandemic Challenges: Responsible Data Use And Infrastructure Application For Surveillance And Pre-Emptive Tracing Post-Crisis, Mark Findlay, Jia Yuan Loke, Nydia Remolina Leon, Yum Yin, Benjamin (Tan Renyan) Tham

Research Collection Yong Pung How School Of Law

As the COVID-19 health pandemic rages governments and private companies across the globe are utilising AI-assisted surveillance, reporting, mapping and tracing technologies with the intention of slowing the spread of the virus. These technologies have the capacity to amass personal data and share for community control and citizen safety motivations that empower state agencies and inveigle citizen co-operation which could only be imagined outside such times of real and present danger. While not cavilling with the short-term necessity for these technologies and the data they control, process and share in the health regulation mission, this paper argues that this infrastructure …

Health Inequalities, Lawrence O. Gostin, Eric A. Friedman

Georgetown Law Faculty Publications and Other Works

The vast health inequalities in the United States and beyond that COVID-19 makes glaringly evident are frequently masked by aggregate statistics, which for years had been showing health improvements. Yet these improvements were inequitably distributed, with benefits disproportionately going to wealthier – and in the United States, white – populations. Globally, vast health inequities also exist among and within countries. The inequalities, which have also helped fuel the rise of populism, extend far beyond health care, including to wealth and income. Disaggregated, granular data is critical to understanding these inequalities.

Addressing health inequities must extend far beyond universal access to …

Investigating The Potential Of Sodium Butyrate To Control Salmonella Enteritidis In Poultry, Anamika Gupta

Graduate Theses and Dissertations

Salmonella Enteritidis (SE) is a major poultry associated food borne pathogen that causes enteric illnesses in humans. Despite using various pre-harvest and post-harvest intervention strategies to reduce Salmonellosis, SE infection is still an extensive problem in the poultry industry with increased incidences since SE has developed multiple strategies to adapt in the chicken intestinal tract particularly in the ceca. Therefore, reducing SE in the intestine of chickens would reduce contamination of poultry derived foods and minimize the risk of human infection. Short chain fatty acids such as butyrate are microbial metabolites known to modulate inflammatory response. In this dissertation, the …

Evaluation Of Anthelmintic Therapies In A Fall Calving Beef Cowherd, Laine Zammit

Graduate Theses and Dissertations

Internal parasitism inevitability prompts economic loss in beef cattle production by decreasing growth performance and reproductive traits. Today, the most widely used class of anthelmintic used to treat parasitism, is the macrocyclic lactone. Many studies have conflicting results on the efficacy of macrocyclic lactones (ML) efficacy against internal parasitism. The objective of this study was to evaluate the effectiveness of moxidectin and eprinomectin, two of the MLs, on cow performance. Multiparous fall calving, crossbred beef cows (n = 106) were allocated randomly to 1 of 3 anthelmintic treatments: 1) Negative control (CON), in which cows did not receive an anthelmintic, …

Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page

Graduate Theses and Dissertations

Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …

Preliminary Incidence And Trends Of Infections With Pathogens Transmitted Commonly Through Food - Foodborne Diseases Active Surveillance Network, 10 U.S. Sites, 2016-2019, Danielle M. Tack, Logan Ray, Patricia M. Griffin, Paul R. Cieslak, John Dunn, Tamara Rissman, Rachel Jervis, Sarah Lathrop, Alison Muse, Monique Duwell, Kirk Smith, Melissa Tobin-D'Angelo, Duc J. Vugia, Joanna Zablotsky Kufel, Beverly J. Wolpert, Robert Tauxe, Daniel C. Payne

Pathology Research and Scholarship

To evaluate progress toward prevention of enteric illnesses, the Foodborne Diseases Active Surveillance Network (FoodNet) of CDC's Emerging Infections Program monitors the incidence of laboratory-diagnosed infections caused by eight pathogens transmitted commonly through food at 10 U.S. sites.* This report summarizes preliminary 2019 data and describes changes in incidence compared with that during 2016-2018. The incidence of enteric infections caused by these eight pathogens reported by FoodNet sites in 2019 continued to increase or remained unchanged, indicating progress in controlling major foodborne pathogens in the United States has stalled. Campylobacter and Salmonella caused the largest proportion of illnesses; trends in …

Embracing The Power Of Show-Me Echo Learning Communities To Transform Clinical Practice In Missouri., Lea Brandt, Melissa Warne-Griggs, Kimberly Hoffman, Lori Popejoy, E Rachel Mutrux

Project ECHO Bibliography

Show-Me ECHO, a state-funded project, provides access to education within a community of learners in order to optimize healthcare for the citizens of Missouri. Through videoconferencing and case-based review, ECHO shifts professional development from learning about medical problems in isolation to experiential learning as part of a multidisciplinary team. The establishment of a statewide COVID-19 ECHO is allowing a rapid response to this novel, unprecedented, and unanticipated health care crisis. There are many ongoing opportunities for clinicians from across the state to join a Show-Me ECHO learning community as a means to elevate their practice and improve ability to respond …

Building Regional Expertise: Perspectives From Show-Me Echo Participants., Melissa Warne-Griggs, Lea Brandt, Kimberly Hoffman, Tracy Greever-Rice, E Rachel Mutrux

Project ECHO Bibliography

This article highlights four primary care providers that practice in underserved areas in Missouri. Show-Me ECHO (Extension for Community Healthcare Outcomes) provides colleagues opportunities for consultation and Continuing Medical Education (CME) around particular conditions. Through their participation, these providers have 1) enhanced their comfort and skill in diagnosis and treatment, 2) become important regional resources for patients and other providers, and 3) improved access to specialty services in their community.

Who And When To Screen: Multi-Round Active Screening For Network Recurrent Infectious Diseases Under Uncertainty, Han-Ching Ou, Arunesh Sinha, Sze-Chuan Suen, Andrew Perrault, Alpan Raval, Milind Tambe

Research Collection School Of Computing and Information Systems

Controlling recurrent infectious diseases is a vital yet complicated problem in global health. During the long period of time from patients becoming infected to finally seeking treatment, their close contacts are exposed and vulnerable to the disease they carry. Active screening (or case finding) methods seek to actively discover undiagnosed cases by screening contacts of known infected people to reduce the spread of the disease. Existing practice of active screening methods often screen all contacts of an infected person, requiring a large budget. In cooperation with a research institute in India, we develop a model of the active screening problem …

Accelerating Care Through Echo: Case Examples From The Field., Mirna Becevic, Emmanuelle Wallach, Lincoln R. Sheets, Hope Misterovich, Susan Norris, Evelyn Aboagye, Jonathan A Dyer, Bruce R Bacon, Karen Edison, Kristin Sohl

Project ECHO Bibliography

In this article, we describe three life-changing patient cases demonstrating high-quality and timely care they received in their communities, thanks to the Show-Me ECHO project. Early autism diagnosis, a potentially deadly tumor manifesting as a benign-looking rash, a recalcitrant case of hepatitis C: rural and underserved Missourians now have access to state-of-the-art care through their local providers receiving interdisciplinary telementoring on evidence based practices.

Battlefield Of Bandages: A Case Study On Sanitation Policy, Medical Reform, And Disease Prevention During The War Of Rebellion, Ashley L. Simpson

Graduate Theses/Dissertations

The American Civil War was a devastating conflict costing over 750,000 lives and millions of dollars in the aftermath. However, the most urgent threat was not musket balls, cannons or grapeshot. Afflictions such as typhoid fever, malaria, smallpox, measles, pneumonia, and diarrhea contracted from crowded, unsanitary camp and hospital conditions were responsible for two-thirds of all Civil War casualties. In April 1861, a group of Union women met at church to organize a relief agency whose goal was to aid the thousands of Union soldiers dying from disease. Armed with enlightenment ideas about medical care and sanitation, the Women's Central …