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Articles 4171 - 4200 of 24031
Full-Text Articles in Entire DC Network
Beyond Books 2: Cataloging Archival Materials, Allie Mccormack, Rebecca A. Wiederhold, Nicole Lewis, Becky Skeen
Beyond Books 2: Cataloging Archival Materials, Allie Mccormack, Rebecca A. Wiederhold, Nicole Lewis, Becky Skeen
Faculty Publications
View the video presentation in two parts at the following links:
Part 1 https://youtu.be/EQEhVpmfOBc
Part 2 https://youtu.be/XIAMdPYspFo
Archival materials can often be found in cultural heritage organizations that do not have trained special collections catalogers on staff. In this workshop, participants will learn how to catalog manuscripts, photographs, and audiovisual materials so they can be discovered in online catalogs and databases. Both item and collection-level cataloging will be discussed and participants will learn when and how to apply metadata standards like RDA and DACS. Time for hands-on practice will be included.
The Diamond, September 25, 2020, Dordt University
The Diamond, September 25, 2020, Dordt University
Dordt Diamond
- Front Page: Dordt Sees a Spike in COVID-19 Cases after Hitting Zero; There's a Battle Brewing at the Harvest Festival; Protests and Police Reform
- News: The Handbook: A Roadmap for Dordt; Sioux Center Small Businesses Moving Forward; The Gift that Keeps on Giving: Dordt Alumni as Professors; Wildfires Rage in the Pacific Northwest
- Art & Entertainment: Social Distancing and the Arts: The Show Must Go On; Dordt University Students Gather for All-Campus Movie; Review: The Devil All the Time; Review: Coming-Of-Age Cliches Done Right
- Feature: Isolated Images: COVID-19 and its Impact on the Film Industry; The …
Impacts Of Microplastic Fibres On The Marine Mussel, Mytilus Galloprovinciallis, Nashami Alnajar, Awadhesh N. Jha, Andrew Turner
Impacts Of Microplastic Fibres On The Marine Mussel, Mytilus Galloprovinciallis, Nashami Alnajar, Awadhesh N. Jha, Andrew Turner
School of Biological and Marine Sciences
No abstract provided.
Modulation Of Rab7a-Mediated Growth Factor Receptor Trafficking Inhibits Islet Beta Cell Apoptosis And Autophagy Under Conditions Of Metabolic Stress, Nirun V. Hewawasam, Fadel Lhaf, Henry A. Taylor, Katrina Viloria, Amazon Austin, Aileen King, P Jones, L Jones, Mark D. Turner, Natasha J. Hill
Modulation Of Rab7a-Mediated Growth Factor Receptor Trafficking Inhibits Islet Beta Cell Apoptosis And Autophagy Under Conditions Of Metabolic Stress, Nirun V. Hewawasam, Fadel Lhaf, Henry A. Taylor, Katrina Viloria, Amazon Austin, Aileen King, P Jones, L Jones, Mark D. Turner, Natasha J. Hill
School of Biomedical Sciences
Abstract Regenerative medicine approaches to enhancing beta cell growth and survival represent potential treatments for diabetes. It is known that growth factors such as insulin, IGF-1 and HGF support beta cell growth and survival, but in people with type 2 diabetes the destructive effects of metabolic stress predominate and beta cell death or dysfunction occurs. In this study we explore the novel hypothesis that regulation of growth factor receptor trafficking can be used to promote islet beta cell survival. Growth factor signalling is dependent on the presence of cell surface receptors. Endosomal trafficking and subsequent recycling or degradation of these …
3d-Printed Leg Design And Modification For Improved Support On A Quadruped Robot, Jasmin S. Collins
3d-Printed Leg Design And Modification For Improved Support On A Quadruped Robot, Jasmin S. Collins
Maseeh Summer Undergraduate Research Experience
The Agile and Adaptive Robotics Lab aims to uncover the biological and physiological complexities in animal agility and adaptive control, which can be replicated through robotics and provide further applications in biology and medicine. One project within the lab focuses on understanding structure, actuation, and control through the modeling of a canine quadruped robot.
The AARL has developed a full-body quadruped robot with artificial muscles that control limb movement and a body that is built from 3D-printed parts. This specific project involved modification of these existing parts to (a) minimize deflections in the front legs, causing unwanted lateral and abduction/adduction …
Atrial Myocyte Nlrp3/Camkii Nexus Forms A Substrate For Postoperative Atrial Fibrillation, Jordi Heijman, Azinwi Phina Muna, Tina Veleva, Cristina E Molina, Henry Sutanto, Marcel Tekook, Qiongling Wang, Issam H Abu-Taha, Marcel Gorka, Stephan Künzel, Ali El-Armouche, Hermann Reichenspurner, Markus Kamler, Viacheslav Nikolaev, Ursula Ravens, Na Li, Stanley Nattel, Xander H T Wehrens, Dobromir Dobrev
Atrial Myocyte Nlrp3/Camkii Nexus Forms A Substrate For Postoperative Atrial Fibrillation, Jordi Heijman, Azinwi Phina Muna, Tina Veleva, Cristina E Molina, Henry Sutanto, Marcel Tekook, Qiongling Wang, Issam H Abu-Taha, Marcel Gorka, Stephan Künzel, Ali El-Armouche, Hermann Reichenspurner, Markus Kamler, Viacheslav Nikolaev, Ursula Ravens, Na Li, Stanley Nattel, Xander H T Wehrens, Dobromir Dobrev
Faculty, Staff and Students Publications
RATIONALE: Postoperative atrial fibrillation (POAF) is a common and troublesome complication of cardiac surgery. POAF is generally believed to occur when postoperative triggers act on a preexisting vulnerable substrate, but the underlying cellular and molecular mechanisms are largely unknown.
OBJECTIVE: To identify cellular POAF mechanisms in right atrial samples from patients without a history of atrial fibrillation undergoing open-heart surgery.
METHODS AND RESULTS: Multicellular action potentials, membrane ion-currents (perforated patch-clamp), or simultaneous membrane-current (ruptured patch-clamp) and [Ca
CONCLUSIONS: Preexisting Ca
Matrine Inhibits Cns Autoimmunity Through An Ifn-Β-Dependent Mechanism, Yaojuan Chu, Wendi Ma, Rodolfo Thome, Jie Dan Ping, Fangzhou Liu, Mengru Wang, Mingliang Zhang, Guang-Xian Zhang, Lin Zhu
Matrine Inhibits Cns Autoimmunity Through An Ifn-Β-Dependent Mechanism, Yaojuan Chu, Wendi Ma, Rodolfo Thome, Jie Dan Ping, Fangzhou Liu, Mengru Wang, Mingliang Zhang, Guang-Xian Zhang, Lin Zhu
Department of Neurology Faculty Papers
Matrine (MAT), a quinolizidine alkaloid component derived from the root of Sophora flavescens, suppresses experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis (MS), by inducing the production of immunomodulatory molecules, e.g., IL-10. In an effort to find the upstream pathway(s) of the mechanism underlying these effects, we have tested certain upregulated immunomodulatory molecules. Among them, we found increased levels of IL-27 and IFN-β, one of the first-line MS therapies. Indeed, while low levels of IFN-β production in sera and type I interferon receptor (IFNAR1) expression in spinal cord of saline-treated control EAE mice were detected, they were significantly …
Wellness In The Helping Professions: Historical Overview, Wellness Models, And Current Trends, Ashley J. Blount, Dalena L. Dillman Taylor, Glenn W. Lambie
Wellness In The Helping Professions: Historical Overview, Wellness Models, And Current Trends, Ashley J. Blount, Dalena L. Dillman Taylor, Glenn W. Lambie
Journal of Wellness
Introduction
Wellness and the concept of holism have rich histories throughout the helping professions. However, Westernized medical models often promote the concept of treatment rather than prevention, limiting the helper’s ability to focus on wellness when working with clients/patients. Therefore, in order to support a re-integration to holistic wellness and the prevention of illness, and re-focus on a wellness ideology, we conducted a thorough theoretical overview of wellness in the helping professions to: (a) provide a historical overview of wellness in helping professions, (b) discuss prominent wellness models, (c) review wellness assessments, (d) present wellness supervision models, and (e) offer …
Creating A Competitive Environment For Defense Aerospace In A Protectionist Multipolar World: A Study Of India And Israel, Shlok Misra, Tanish Jain
Creating A Competitive Environment For Defense Aerospace In A Protectionist Multipolar World: A Study Of India And Israel, Shlok Misra, Tanish Jain
Beyond: Undergraduate Research Journal
The paper studies protectionism in defense aerospace in a multipolar world and analyzes the strategies of two emerging powers: India and Israel. The emergence of protectionism in a multipolar world has left a visible and influential impact in the globally integrated defense industry. As the world has become increasingly multipolar, new military powers have emerged around the world. India and Israel are disparate in terms of their size, wealth, and international relations. There are interesting similarities between them when it comes to their defense strategies. As a result, they also present compelling case studies for understanding protectionism in a multipolar …
Editing My Own Drum, Adrienne Adams
Editing My Own Drum, Adrienne Adams
Artizein: Arts and Teaching Journal
The author examines her art and poetry practice exploring how the "Badlands" of Alberta, Canada in particular Áísínai'pi (Writing-on-Stone Provincial Park), are "the bones of the earth". She looks at the visual and linguistic poetry within them and examines her wording to decolonize her practice and learning/unlearning from the land and the cultures; Blackfoot and Settler, and peoples that inhabit it. She highlights how her process of editing a specific poem “My Own Drum” prompted and echoes an examination of her practice that leads to the writing of a new poem written during this process, revealing some of her findings. …
The Verge: Networks Of Intersubjective Responding For Just Sustainability Arts Educational Research, Marna Hauk, Amanda Rachel Kippen
The Verge: Networks Of Intersubjective Responding For Just Sustainability Arts Educational Research, Marna Hauk, Amanda Rachel Kippen
Artizein: Arts and Teaching Journal
Two sustainability arts scholars describe a method of data interpretation they developed for making sense of complex environmental and sustainability education research data. They “played” images and recorded a conversation in a form of arts-based intersubjective knowing. The card game process was named the Verge because of how the process promises to surface unheard voices and re-center nondominant insights and ways of knowing. It leverages Casey’s glance method with systems networks to complicate sense making in arts-based educational research. The arts scholars intermixed research data from two just sustainability education research case studies: collages from participants of a climate justice …
An Unlikely Correspondence: Gps And Body In Place, Patti Pente
An Unlikely Correspondence: Gps And Body In Place, Patti Pente
Artizein: Arts and Teaching Journal
This visual essay investigates the significance of GPS technology and the human body in relation to a sense of place. Framed by posthumanism, the poetic images offer an approach to transformative education through a local site. Agency of place is key to this exploration of dynamic relationships with technology, body and the earth. As a creative performance, I executed a series of movements in a place of personal significance, and then further developed the essay through visual poetry. The research is informed by an underlying assumption that creative understanding and artistic analysis can foster deeper environmental care, and although this …
Lessons From Birds, Bones, And The Body, Alexandra Fidyk, Darlene St.Georges
Lessons From Birds, Bones, And The Body, Alexandra Fidyk, Darlene St.Georges
Artizein: Arts and Teaching Journal
Written as a layered pictorial script, three images—Owl, Raven, and Hummingbird—beckoned the authors. Together, they explored neglected epistemologies in many disciplines within education, the arts and humanities to deepen their understanding of what it means to live in caring relation with an animated world. By tracking the Birds’ lineages and kinships through active imagination, amplification, and circumambulation, concealed patterns and associations reveal lessons for living more consciously with repressed and expressed feminine/androgyne energies. To be more conscious of both aids in the unfolding processes central to becoming, undoing, creativity, and pedagogy. Herein an ethical challenge arises: how might we honour …
Eco-Pedagogical Wandering And Pondering In Pacific Spirit Park, Nicole Rallis
Eco-Pedagogical Wandering And Pondering In Pacific Spirit Park, Nicole Rallis
Artizein: Arts and Teaching Journal
This poetry emerges from my ongoing Ph.D. research and coursework at the University of British Columbia, where I am engaging in an a/r/tographic-walking inquiry on environmental sustainability and climate change. After moving to the unceded and ancestral lands of the Coast Salish peoples, now known as Vancouver, I began walking the forest trails of Pacific Spirit Park. Inspired by Robin Wall Kimmerer’s (2013) pedagogical discussions about the grammar of animacy, two-eyed ways of seeing, and childlike ways of seeing, my poems pay tribute to the plant elders and more-than-human beings guiding my learning journey. My ecopedagogical wandering and pondering align …
A Contemplative And Artful Métissage Of Inquiry And Response, Jackie Mitchell, Nicholas Phillips, Robyn Trail, Susan C. Walsh, Barbara Bickel, Wendalyn Bartley, Medwyn Mcconachy
A Contemplative And Artful Métissage Of Inquiry And Response, Jackie Mitchell, Nicholas Phillips, Robyn Trail, Susan C. Walsh, Barbara Bickel, Wendalyn Bartley, Medwyn Mcconachy
Artizein: Arts and Teaching Journal
In this mixed media métissage, we offer an exploration of artful and contemplative inquiry and response. We are a group of seven artist-researchers who engage with contemplative practices associated with various spiritual traditions, including spiritual feminist, Wiccan, Mi’kmaw, and Tibetan Buddhist, integral to all of which are beliefs about human interconnectedness with the energies of all sentient beings, the Earth, and beings in the spirit worlds. As artist-researchers, we engage with a range of arts disciplines including poetry, creative non-fiction, storytelling, sounding, visual art, filmmaking, and photography. Together, we invite the reader/listener/viewer--as co-creator—into the potentialities of our métissage: the narratives, …
Paying It Forward: A Gift Economy Of Poetry And Visual Art Images, Susan Gerofsky, Daniel Barney, Mira Gerard
Paying It Forward: A Gift Economy Of Poetry And Visual Art Images, Susan Gerofsky, Daniel Barney, Mira Gerard
Artizein: Arts and Teaching Journal
As our world has changed rapidly and ineluctably with the COVID-19 pandemic, many are advocating an ethos of generosity and a gift economy, based on generative, creative offerings, as an alternative or balance to the excesses of a mainstream neoliberal exchange economy. What is the gift economy, and how does it entangle us in a fabric of mutual responsibility, obligation, creative practices and love, within the human and greater-than-human world? A Pay-It-Forward New Year's gift game amongst a group of artist/ educators, ongoing since 2014, gives rise to this meditation on the gift economy, based on Mauss, Hyde, Kimmerer, Vaughan …
Full Issue Artizein October 2020, Barbara Bickel, Darlene St.Georges
Full Issue Artizein October 2020, Barbara Bickel, Darlene St.Georges
Artizein: Arts and Teaching Journal
Full PDF of the 2020 issue edited by Barbara Bickel with Guest Assistant Editor & Design by Darlene St. Georges
Non-Respiratory Presentations Of Covid-19, A Clinical Review., Marya Alsamman, Amy Caggiula, Sangrag Ganguli, Monika Misak, Ali Pourmand
Non-Respiratory Presentations Of Covid-19, A Clinical Review., Marya Alsamman, Amy Caggiula, Sangrag Ganguli, Monika Misak, Ali Pourmand
COVID-19 Publications by GW Authors
INTRODUCTION: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or COVID-19) is a highly infectious viral syndrome currently threatening millions of people worldwide. It is widely recognized as a disease of the pulmonary system, presenting with fever, cough, and shortness of breath. However, a number of extrapulmonary manifestations have been described in the literature.
OBJECTIVE: In this review, we seek to provide a comprehensive summary of the hematologic, gastroenterological, renal, dermatologic, neurologic, and psychiatric manifestations of COVID-19.
DISCUSSION: Hematological presentations of COVID-19 include laboratory abnormalities such as decreased total lymphocyte count, prolonged prothrombin time (PT), elevated d-dimer, and increased lactate dehydrogenase …
A Naturalistic Ethic Supporting A Vegan Diet, Michael Morris
A Naturalistic Ethic Supporting A Vegan Diet, Michael Morris
Between the Species
Nutritional evidence suggests that a vegan diet is the most adaptive one for humans. An ethical principle based on following our biological nature (naturalistic ethic) could therefore provide additional support for a vegan diet. However, some argue that humans in the natural world could not eat a vegan diet, since it relies on supplements, particularly vitamin B12. This leads to the conclusion that humans are naturally omnivores, and therefore our natural diet should include small amounts of animal products.
Three approaches to this conclusion are discussed. The first rejects a naturalistic ethic in favour of normative principles based on animal …
General Psychology For Honors Students, Kate Votaw
General Psychology For Honors Students, Kate Votaw
Open Educational Resources Collection
What are the most effective methods to study for a test? What are the meanings of dreams? How do illusions work? With whom are you most likely to fall in love? These are just a few of the questions that have been asked by psychologists since the birth of the field as an area of scientific research in the 1870’s. This text surveys the basic concepts, theories, and pivotal findings over the past 100 years in the science of Psychology, with special emphasis on contemporary concepts and findings focused on the relation of the brain to normal and pathological behaviors. …
Evangelism - Sharing The Gospel At Work (Overview), Theology Of Work Project, Bill Peel, Leah Archibald, Randy Kilgore
Evangelism - Sharing The Gospel At Work (Overview), Theology Of Work Project, Bill Peel, Leah Archibald, Randy Kilgore
Theology of Work Project
The Indiana Statesman, September 24, 2020, Indiana State University
The Indiana Statesman, September 24, 2020, Indiana State University
Student Newspaper
Celebrating intellectual freedom; COVID-19 surge caused by Labor Day; Officer indicted in Breonna Taylor case; Schumer seeks to block court replacement before Election Day
Neutrophil: A New Player In Metastatic Cancers, Mengyue Wu, Mutian Ma, Zhenya Tan, Hong Zheng, Xia Liu
Neutrophil: A New Player In Metastatic Cancers, Mengyue Wu, Mutian Ma, Zhenya Tan, Hong Zheng, Xia Liu
Toxicology and Cancer Biology Faculty Publications
The interaction between cancer cells and immune cells is important for the cancer development. However, much attention has been given to T cells and macrophages. Being the most abundant leukocytes in the blood, the functions of neutrophils in cancer have been underdetermined. They have long been considered an “audience” in the development of cancer. However, emerging evidence indicate that neutrophils are a heterogeneous population with plasticity, and subpopulation of neutrophils (such as low density neutrophils, polymorphonuclear-myeloid-derived suppressor cells) are actively involved in cancer growth and metastasis. Here, we review the current understanding of the role of neutrophils in cancer development, …
Mesencephalic Astrocyte-Derived Neurotrophic Factor (Manf) Regulates Neurite Outgrowth Through The Activation Of Akt/Mtor And Erk/Mtor Signaling Pathways, Wen Wen, Yongchao Wang, Hui Li, Hong Xu, Mei Xu, Jacqueline A. Frank, Murong Ma, Jia Luo
Mesencephalic Astrocyte-Derived Neurotrophic Factor (Manf) Regulates Neurite Outgrowth Through The Activation Of Akt/Mtor And Erk/Mtor Signaling Pathways, Wen Wen, Yongchao Wang, Hui Li, Hong Xu, Mei Xu, Jacqueline A. Frank, Murong Ma, Jia Luo
Pharmacology and Nutritional Sciences Faculty Publications
Neurite outgrowth is essential for brain development and the recovery of brain injury and neurodegenerative diseases. In this study, we examined the role of the neurotrophic factor MANF in regulating neurite outgrowth. We generated MANF knockout (KO) neuro2a (N2a) cell lines using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and demonstrated that MANF KO N2a cells failed to grow neurites in response to RA stimulation. Using MANF siRNA, this finding was confirmed in human SH-SY5Y neuronal cell line. Nevertheless, MANF overexpression by adenovirus transduction or addition of MANF into culture media facilitated the growth of longer neurites in RA-treated N2a …
Whalesong 2020-09-24 (V.40 No.4), Mike Flunker, Baylee Schneider, Kenedy Williams, Sierra Lissick, Rosemarie Alexander, Heidi Pearson
Whalesong 2020-09-24 (V.40 No.4), Mike Flunker, Baylee Schneider, Kenedy Williams, Sierra Lissick, Rosemarie Alexander, Heidi Pearson
Whalesong
No abstract provided.
Roosting Ecology Of A Declining Population Of Northern Long-Eared Bats (Myotis Septentrionalis) In The Ozark-St. Francis National Forest Of Arkansas, Kyle Norris Edmonds
Roosting Ecology Of A Declining Population Of Northern Long-Eared Bats (Myotis Septentrionalis) In The Ozark-St. Francis National Forest Of Arkansas, Kyle Norris Edmonds
Student Theses and Dissertations
Ecological and monetary values associated with bat species are immense. Unfortunately, these benefits are at risk as disease and habitat loss have resulted in significant declines of bat populations throughout the eastern United States. Wildlife biologists and managers work tirelessly to combat the effects of these threats. Collections of species-level data during surveys provide managers with invaluable information that can be used in identification of emerging population trends. This material can then be used to generate new habitat management plans or revise existing plans in order to address the objectives of primary management goals. This thesis outlines anthropogenic factors, interspecific …
Global Convergence Of Covid-19 Basic Reproduction Number And Estimation From Early-Time Sir Dynamics, Gabriel G. Katul, Asaad Mrad, Sara Bonetti, Gabriele Manolia, Anthony J. Parolari
Global Convergence Of Covid-19 Basic Reproduction Number And Estimation From Early-Time Sir Dynamics, Gabriel G. Katul, Asaad Mrad, Sara Bonetti, Gabriele Manolia, Anthony J. Parolari
Civil and Environmental Engineering Faculty Research and Publications
The SIR (‘susceptible-infectious-recovered’) formulation is used to uncover the generic spread mechanisms observed by COVID-19 dynamics globally, especially in the early phases of infectious spread. During this early period, potential controls were not effectively put in place or enforced in many countries. Hence, the early phases of COVID-19 spread in countries where controls were weak offer a unique perspective on the ensemble-behavior of COVID-19 basic reproduction number inferred from SIR formulation. The work here shows that there is global convergence (i.e., across many nations) to an uncontrolled Ro=4.5 that describes the early time spread of COVID-19. This value …
Prevalence And Determinants Of Anemia Among Women Of Reproductive Age In Thatta Pakistan: Findings From A Cross-Sectional Study, Sumera Aziz Ali, Zahid Abbasi, Babar Shahid, Ghazal Moin, K Michael Hambidge, Nancy F. Krebs, Jamie E. Westcott, Elizabeth M. Mcclure, Robert L. Goldenberg, Sarah Saleem
Prevalence And Determinants Of Anemia Among Women Of Reproductive Age In Thatta Pakistan: Findings From A Cross-Sectional Study, Sumera Aziz Ali, Zahid Abbasi, Babar Shahid, Ghazal Moin, K Michael Hambidge, Nancy F. Krebs, Jamie E. Westcott, Elizabeth M. Mcclure, Robert L. Goldenberg, Sarah Saleem
Community Health Sciences
Background: Anemia is a major public health concern among women of reproductive age leading to high maternal mortality in low- and middle-income countries. Of the prior studies conducted in Pakistan, most focused on large urban areas and did not explore the determinants of anemia among women of reproductive age (WRA) across socio-demographic, dietary, reproductive, and biological domains. Thus, we aimed to study the prevalence and determinants of anemia among WRA in rural Pakistan.
Methods: We conducted a cross-sectional study in the Thatta district of Pakistan from September 2018 to January 2019 and enrolled 150 non-pregnant, married women. Data collectors administered …
Malnutrition And Food Insecurity In Child Labourers In Sindh, Pakistan: A Cross-Sectional Study, Meesha Iqbal, Zafar Fatmi, Kausar S. Khan, Yusra Jumani, Neelma Amjad, Asaad Ahmed Nafees
Malnutrition And Food Insecurity In Child Labourers In Sindh, Pakistan: A Cross-Sectional Study, Meesha Iqbal, Zafar Fatmi, Kausar S. Khan, Yusra Jumani, Neelma Amjad, Asaad Ahmed Nafees
Community Health Sciences
Background: Child labour is common in low- and middle-income countries. Although child labour is widespread in Pakistan, no data are available on the health of child labourers.
Aims: This study aimed to assess the food security, food intake and nutritional status of child labourers aged 5-14 years working in lower Sindh, Pakistan.
Methods: Child labourers aged 5-14 years working in agriculture, manufacturing industry, hotels and restaurants, domestic work and migrant child labourers working in vegetable markets were recruited using a respondent-driven sampling technique. Sociodemographic and nutrition information was obtained by an interviewer questionnaire. The children's height and weight were measured …
Choice Of Molecular Assay Determines Ranavirus Detection Probability And Inferences About Prevalence And Occurrence, Felicity J. Wynne, Robert Puschendorf, Mairi E. Knight, Stephen J. Price
Choice Of Molecular Assay Determines Ranavirus Detection Probability And Inferences About Prevalence And Occurrence, Felicity J. Wynne, Robert Puschendorf, Mairi E. Knight, Stephen J. Price
School of Biological and Marine Sciences
Ranaviruses are emerging pathogens that can cause morbidity, mortality and population declines in ectothermic hosts; however, there is no standardized approach to diagnostics. Here, we compared the inter-assay variation and intra-assay precision among 2 commonly used quantitative PCRs (qPCRs), a conventional and a nested PCR assay (used as a gold standard), using laboratory-propagated ranavirus (FV3 and CMTV) and field-collected samples. A qPCR assay (‘Leung’) detected viral DNA in dilutions 2 orders of magnitude lower than other assays regardless of the viral lineage of the cultured isolate (FV3/CMTV). The second qPCR (‘Brunner’) was slightly more sensitive than the conventional PCR (‘Mao’ …