Digital Commons Network^™

Regulation Of Vascular And Lymphatic Endothelial Barrier Function By Sphingosine-1-Phosphate, Jacqueline Bellaci

Theses and Dissertations

Endothelial cells play an important role in maintaining homeostasis, and disruption of the endothelial barrier have been linked to a number of different pathologies, including cancer metastasis. The vascular endothelial barrier is known to be regulated by a variety of factors, including sphingosine-1-phosphate (S1P), a bioactive lipid metabolite, which has been shown to improve barrier function. However, most studies to date have been focused on the vascular endothelium, and the regulation of the lymphatic endothelial barrier has not been thoroughly investigated. We found that prior to any stimulation the lymphatic endothelial cells (LECs) had a lower trans-endothelial resistance (TER) in …

Martian Mother, Elizabeth Mcgrady

Theses and Dissertations

This paper examines the relationship between humans and land, through the lens of the scientific and religious, bridging the physical realm with the spiritual. It acts as accompanying material to the project titled Martian Mother, supplementary information to the visual work, and an extension of the proposal, the center of the work. The proposal exists to send myself, or a like-minded individual, to Mars with artificial insemination equipment to give birth to the first Martian, becoming the first Martian Mother. This work is rooted firmly in speculative fiction, creating a nonlinear future framework for a new society and space exploration.

Spit In My Mouth: Queer Intimacies, Material Intra-Actions, And Sensuous Becoming, Gm Keaton

Theses and Dissertations

This document describes my multidisciplinary art practice as it intersects with New Materialism, Queer and Affect theory, Ecology, and my embodied and experiential knowledge as a queer subject. The writing is divided into two categories. One is more theoretical, thinking through these different discourses. The other realizes them through relationships and intra-actions between my material kin and me. With these two modes of writing,I propose that embodied and felt knowing is as valid and illuminating as more traditional forms of knowledge. These sections are interdependent and resist linear logic, offering relational meanings to each reader as they find their way …

A Girl Is A Girl Is A Girl, Paige Lizbeth Morris

Theses and Dissertations

It is well known that Western society uses men’s visual pleasure as a means to determine womxn’s value; her success is dependent on her compliance with culturally determined beauty standards. This process turns womxn into commodities and reinforces notions of gender complementarity, perpetuating a lifetime of servitude to patriarchal ideologies. It reminds the non-male that she is incomplete, insufficient, insignificant, and so easily dismissable. It forces her to be dependent, to shut up, to sit down, and to enjoy it.

In an effort to reject these presumed histories and narrow connotations of womxn, I have analyzed my gender performance in …

Prey Begins With Play, Seren C. Moran

Theses and Dissertations

My work examines the lasting psychological and emotional torment of trauma focusing on sexual violence against women. My thesis consists of five short animated films recounting non-fiction experiences of sexual assault. To contextualize my work within cultural and historical representations of sexual violence, this text reflects my research on the mishandling of rape in Western art history as well as contemporary politics and cinema. In both my work and research, I address the complicated, and often contradictory, internal reality of experiencing and remembering trauma and how this relates to artistic strategies such as collage and maximalism. My personal struggles recovering …

Structural Features Contributing To Methylenedioxypyrovalerone (Mdpv) Analog Inhibition Of The Human Dopamine Transporter, Zachary R. Spires

Theses and Dissertations

Monoamine transporters (MATs) are transmembrane neurotransmitter sodium symporters consisting of the dopamine, norepinephrine, and serotonin transporters: DAT, NET, and SERT, respectively. Following an action potential and neurotransmitter vesicular release, MAT-mediated reuptake of these neurotransmitters in the presynaptic membrane contributes to the termination of monoaminergic neurotransmission. MATs are the targets of many drugs providing therapeutic benefits or taken recreationally. One of these drugs is methylenedioxypyrovalerone (MDPV), an abused synthetic cathinone that is a high potency uptake inhibitor of the human DAT (hDAT). In contrast, MDPV is a low potency uptake inhibitor of the Drosophila melanogaster dopamine transporter (dDAT). The reasons for …

Ghost Signs Are More Than Paintings On Brick, Eric Anthony Berdis

Theses and Dissertations

My work embraces a maximalist aesthetic that incorporates, archival research, personal secrets, and pubescent gay boy glamour. I seek to create a stimulating yet jarring experience, while building a world that is both familiar and inherently strange to the viewer. Thrift store cast-offs, hobbyist craft supplies, and saturated drawings are reassembled into a cast of characters and costumes that balance on the line between ghosts, creatures, and friends. While we often think of costume and even art installations as meant to cover bodies and walls, my work tends to reveal more than conceal. I aspire through this work to shine …

Arabic Beyond Arabic, Abir Zakzok

Theses and Dissertations

Arabic is the best and the most complicated language of all time!” Even though this statement seems like an exaggeration, it is what I grew up hearing; from my Arab parents as a child, my Arab teachers as a student, and my Arab customers as a salesperson. The Arabic language has a significant role in Arabs’ identity, yet most Arabs only scratch the surface and do not fully grasp the embedded meaning of the language. I have long been fascinated by the relationship between language and community as well as how it translates to design - specifically, the relationship between …

Machine Girl, Yutong Liu

Theses and Dissertations

Machine girl is a character that mimics biology, labor, and humanity.

She relates to machine sympathy and machine learning; She is updating and processing of big data. During the interaction between me and the machine, I found you --- Machine girl.

Machine girl is a brilliant girl. She is an excellent translator and communicator, which is determined by her ability for machine learning. The cyber living space gives her a fundamental learning gift with a mathematical algorithm. She uses this gift to mimic everything from the human brain. She is not reasonable but sensitive. There are many different versions of …

Fool's Rest, Aidan Quinlan

Theses and Dissertations

The text calls itself a guide, but it is no guide. From the genre of guide, it borrows the outlines and silhouettes and lines between things and spaces and ideas. It lingers on the shadows of its subject(s); it is attentive to the periphery of its world; but it cannot take you there. There is no there. It lacks the authority of the guide; it lacks the lucidity, the efficiency, the false totality of the guide; it lacks the confidence.

The guide embodies its own anxieties. This makes sense, as its reason to exist was born on the one-way highway …

Library By Day, Theater By Night, Julia Moncure Jones Mishler

Theses and Dissertations

This project will re-conceive a historic movie theater, honoring the program of the building as it was originally intended, while introducing a new programmatic element: the library. Instead of this large space only being used in the evenings for movie showings, it will now also be a place for the public to gather during the day as a popular materials library. This project is an exploration of diversion through literature and cinema. This space will continue to be a landmark in Richmond and a place community members visit on a regular basis.

How do new elements introduced into a historic …

Development Of Dexamethsone Sodium Phosphate Loaded Nanoparticles For Treating Corneal Allograft Rejection, Vineet R. Kulkarni

Theses and Dissertations

Corneas are the most commonly transplanted tissue worldwide, with approximately 80,000 transplantations performed in the United States in 2018 alone. Corneal transplantation is used to restore the vision due to opaque corneas in various diseases such as fungal keratitis, keratoconus, Fuchs’s dystrophy, corneal ulceration, traumatic injury, abrasions or scarring, inflammation, and infection. Corneal transplant surgery could involve replacing the entire cornea (penetrating keratoplasty) or selective replacement of the corneal layers (e.g. Descemet’s membrane endothelial keratoplasty and deep anterior lamellar keratoplasty). Immunologic graft rejection is one of the main causes of graft failure in these corneal transplantations. The 2-year rejection rate …

Reaction Profiling Of Extracellular Protein Phosphorylation Through A Microfluidic Reactor Coupled With Raman Spectroscopy, Abigail Casey

Theses and Dissertations

Diseases and disorders in the human body are considered abnormalities of proper cellular function. Understanding the signal transduction mechanisms that cause these abnormalities is crucial to developing earlier detection methods, better treatment options and effective cures. While current diagnostic procedures are powerful tools in diagnosing diseases, they are ineffective in informing physicians on the real-time behavior of the signal transduction mechanisms associated with diseases and disorders. Currently, disease progression is monitored over time through routine patient visits and testing by one or more of the above techniques. By developing an approach that can monitor structural and conformational changes of proteins …

Dendrimer-Based Therapeutic Delivery Vehicle For The Treatment Of Ocular Diseases, Remy C. Cooper

Theses and Dissertations

The treatment of ocular diseases poses a significant challenge to drug delivery design, due to the eye’s complex anatomy, with a semi-permeable blood-ocular barrier and associated immune privilege. To address some of the delivery challenges, polycationic dendrimers are explored as vehicle for the treatment of posterior chamber disease. Dendrimers are a class of polymeric nanoparticles that are highly branched, multivalent and monodisperse, whose three-dimensional globular architecture has given them the nickname of “synthetic proteins”. Dendrimers are synthesized by reacting monomers in a step-wise fashion to produce generationally and radially increasing structures, with core, interior and surface chains that are highly …

Characterization Of The Gonococcal Calprotectin Zn-Acquisition System, Tdfh, And The Efficacy Of Metal Starvation For Treating Gonococcal Disease, Michael T. Kammerman

Theses and Dissertations

Abstract

Characterization of the Gonococcal Calprotectin Zn-Acquisition System, TdfH, and the Efficacy of Metal Starvation for Treating Gonococcal Disease.

By Michael T. Kammerman, B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University

Virginia Commonwealth University, 2020

Major Director: Cynthia Nau Cornelissen, Ph.D.

Professor, Department of Microbiology and Immunology

Neisseria gonorrhoeae, the bacterial agent that is responsible for the human disease gonorrhea, has had a steady increase in the number of infections per year. In 2018 the WHO estimated over 87 million infections occurred world-wide, and the CDC …

Surrogate Memories In Animation And Sound, Jared Duesterhaus

Theses and Dissertations

A document in support of my exploration into memory in relation to the mediums of animation, sound, and theater. A reflection on remembering as a creative act.

It’S Funny Because It’S True: The Transmission Of Explicit And Implicit Racism In Internet Memes, Tabitha Fairchild

Theses and Dissertations

Video games are traditionally seen as the domain of heteronormative white males. The purpose of this study is to explore how racism is transmitted and reproduced within digital communities, and how humor is being used to frame racist discourse in virtual spaces. Digitally, internet memes are widely used rhetorical vehicles, reaching large and broad audiences. The roles these artifacts play in the reproduction and transmission of racist ideology is often obfuscated by the perception that internet memes are "just jokes." This study analyzes internet memes collected from 16 months of communication between members of Discord servers that self-identify as communities …

Investigation Into The Reaction Profile Of A Bioinspired Nickel Complex, Denver Heitger

Theses and Dissertations

Metal complexes that can activate natural oxidants like H₂O₂ and O₂ have become a key research topic in many labs in hope of mimicking nature, by generating a bioinspired complex that can perform unique chemistry through green processes. To prepare the perfect catalyst, this has lead scientists to explore numerous avenues, including the use of both natural and artificial oxidants, to tease apart the reaction pathway taking place. A ligand system dear to the Lucas Lab (13-DOB) has been metalated with nickel, a cheap and earth abundant transition metal. Our bioinspired metal-(di)oxygen adducts can be formed …

The Quantified Elf, Umico Niwa

Theses and Dissertations

The Quantified Elf is an ongoing collection of scientific, medical, archaeological articles and excerpts from my personal journal. It is a way by which I explore the various ways Western notions of personhood subsume human life into constructs of sexuality and gender, overlooking the various other modes of unbridled existence: plant, microbial. fungal, animal, celestial bodies. The speculative medical papers propose novel forms of body modification to combat gender dysphoria.

A Story About A Girl With Snakes For Hair, Kate M. Turner

Theses and Dissertations

My work is autobiographical. I use various art making processes to create a visual archive of my life. By abstracting these memories and experiences, I can examine how culture works surrounding issues of identity. This is my story.

The Impact Of Aging And Mechanical Injury On Alveolar Epithelial And Macrophage Responses In Acute Lung Injury And Inflammation, Michael S. Valentine

Theses and Dissertations

Patients with severe lung pathologies, such as Acute Respiratory Distress Syndrome (ARDS), often require mechanical ventilation as a clinical intervention; however, this procedure frequently exacerbates the original pulmonary issue and produces an exaggerated inflammatory response that potentially leads to sepsis, multisystem organ failure, and mortality. This acute lung injury (ALI) condition has been termed Ventilator-Induced Lung Injury (VILI). Alveolar overdistension, cyclic atelectasis, and biotrauma are the primary injury mechanisms in VILI that lead to the loss of alveolar barrier integrity and pulmonary inflammation. Stress and strains during mechanical ventilation are believed to initiate alveolar epithelial mechanotransduction signaling mechanisms that contribute …

Epigenetic Regulation Of Drug Metabolizing Enzymes In Normal Aging, Mohamad M. Kronfol

Theses and Dissertations

Geriatric populations are at a higher risk for adverse drug reactions (ADRs). This may be partly due to changes in drug metabolism in old age, but the underlying mechanisms are poorly understood. Prior research in humans and mice has shown age-associated changes to the expression of several genes involved in drug metabolism. Furthermore, studies of human blood showed that epigenetic regulation of genes encoding drug metabolizing enzymes change with age. However, it is unknown if genes in the liver are similarly affected. Therefore, we hypothesize that genes encoding drug metabolizing enzymes may show differential epigenetic regulation in the liver with …

Signaling Mechanism Responsible For 5-Ht2a Receptor Tolerance To Psychedelic Induced Head-Twitch Behavior In Mice, Audrey R. Mcmurtrie

Theses and Dissertations

In recent years, there has been a growing interest in psychedelic compounds stemming from their promising use as therapeutic agents and research tools that can be used to treat and study several neuropsychiatric disorders. Psychedelics have proven useful in a broad range of these diseases - they serve as models for psychosis in schizophrenia but have also had promising results in treating major depressive disorder, anxiety, and other common disorders. Decreased stigmatization surrounding psychedelics has further increased their use in research and clinical settings. In light of these trends and the promising nature of their use, a thorough understanding of …

Language-Supportive Strategies And Their Associations With Child Language Outcomes During Instructional Time, Erin S. Wallace

Theses and Dissertations

In this dissertation, I conducted a correlational study that examined the frequency of educator-delivered language-supportive strategies during instructional time in public Kindergarten classrooms. Additionally, I explored the association between educators’ use of language-supportive strategies and child language outcomes. To index child language, I assessed a sample of 96 children from 10 different classrooms on standardized measures of expressive and receptive language. I recruited 10 educators to participate in the present study and assessed their use of language-supportive strategies during instructional time in the classroom through transcription and coding of audio recordings. The educators completed demographic forms for themselves as well …

Novel Bayesian Techniques For Dose Response Data, Faten Alamri

Theses and Dissertations

All forms of life are being exposed to different levels of harmful chemicals that can cause various and serious side effects. Toxicological experiments enable researchers to test these chemicals on animal to determine their major effects. Although optimal and acceptable dose levels have been investigated, researchers continue to strive to minimize side effects and the chemical dosages. Dose-response models and other benchmark approaches play a role in determining the acceptable exposure levels of hazardous chemicals. Parametric techniques, used to determine the tolerable dosages, utilizing ANOVA and non-linear regression models are well represented in the literature. We determined the benchmark dose …

Novel Duel Mtor Inhibitors/Ampk Activators Improve Therapeutic Efficacy Of Doxorubicin And Ameliorate Its Associated Cardiotoxicity In Mice, Sahak Eric Hovsepian

Theses and Dissertations

Triple-negative breast cancer (TNBC) is a particularly aggressive subset of breast cancer that has a molecular expression profile which lacks the estrogen receptor (ER), progesterone receptor (ER), and human epidermal growth factor receptor type 2 (HER2). No targeted treatment options currently exist for TNBC, unlike other types of breast cancer, and therefore survival rates are far lower for these patients. The current treatment for TNBC is systemic anthracycline chemotherapy, typically doxorubicin (DOX), which has great clinical efficacy in increasing survival. However, DOX induces a dose-dependent progressive cardiomyopathy, which can present years and up to decades after the last treatment. This …

Effects Of Gestational Ozone Exposure On Privileged Placental And Brain Barrier Integrity, Alexander I. Hamm

Theses and Dissertations

Ambient outdoor ozone, a common of component of photochemical smog and urban air pollution, is linked to various neurological and vascular pathologies. Its immediate reaction with lung surfactant after inhalation results in complete reactivity of the gas, with no active ozone passing into circulation. This indicates the presence of secondary and tertiary mediators in ozone-related systemic pathologies after pulmonary insult. In vasculature, ozone exposure is associated with an acute hypertensive phenotype apparent at least 24 hours after dose, such as experienced on a hot summer afternoon in a large metropolitan area like Los Angeles or Mexico City. However, the effects …

Mef2-Bound Genes May Influence Ethanol Sedation In Drosophila Melanogaster, Katlyn M. Myers

Theses and Dissertations

Alcohol Use Disorder (AUD) is a global health issue that affects millions of people every year. This disorder has serious negative mental and physical consequences. Currently, treatment options for this disorder are largely limited to psychological therapy, with very few medications available to treat it. Being able to identify the environmental and genetic components that influence AUD can help improve diagnosis and treatment options. Previous studies in humans have shown a link between initial sensitivity and risk for alcohol abuse. Our laboratory uses Drosophila melanogaster as a model to study the genetic and environmental components of alcohol-related behaviors. Previous lab …

An Identification Of Tgf-B'S Role In Ischemic Cholangiopathy, Courtney Chmielewski

Theses and Dissertations

Organ transplants are a necessary intervention for many diseases that result in end stage organ failure. The donation pool cannot match the demands of the transplant list, so expanding the pool to include donation after cardiac death (DCD) is desired. However, there are increased odds of graft failure and ischemic cholangiopathy leading to inferior outcomes when DCD livers are used. Ischemic cholangiopathy consists of multiple diffuse strictures and fibrosis of the bile ducts leading to a loss of epithelialization and fibrosis. The cellular mechanism is proposed to be epithelial-to-mesenchymal transition (EMT). TGF-�� is seen as a key initiator of EMT, …

Modeling Gardnerella Host-Pathogen Interaction: Characterization Of Vaginolysin And The Opacity Phenotype, Erin M. Garcia

Theses and Dissertations

Bacterial vaginosis (BV) describes a dysbiotic state of the vaginal mucosa, during which the dominance by beneficial lactobacilli species is lost, the vaginal pH increases, and the flora comprising the vaginal microbiome becomes more diverse. Bacterial taxa associated with this dysbiotic state interact to form a dense polymicrobial biofilm on the surface of the vaginal epithelium. This shift in the vaginal microflora is clinically important, even when symptoms are not noted, as women with BV are at increased risk for acquisition of other sexually transmitted infections, including HIV, and are more than two times as likely to experience preterm birth. …