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Evaluating Methods Of Obtaining Male Pheromone From Hymenochirus Sp. Using Analytical Chemistry, Vincent Wing-Kun Leung

University of the Pacific Theses and Dissertations

Male Hymenochirus sp. frogs are known to release pheromone that attracts females of the same species. Four methods for collecting secretions containing pheromone in Hymenochirus sp. were tested: norepinephrine injection, gonadotropin-releasing hormone injection, homogenization of gland tissue, and electrostimulation of the skin over the breeding gland area. The samples collected were analyzed using high-performance liquid chromatography (HPLC) and mass spectrometry. The HPLC chromatograph for the male norepinephrine sample contained a peak at 6.4 min that was not in the female norepinephrine sample HPLC chromatograph. The male norepinephrine sample mass spectrum had a peak of m/z 292.0 not in the female …

Blubber Transcriptome And Proteome Responses To Repeated Adrenocorticotropic Hormone Administration In A Marine Mammal, Jared Deyarmin

University of the Pacific Theses and Dissertations

Chronic physiological stress impacts animal fitness by catabolizing metabolic stores and suppressing reproduction and immunity. This can be especially deleterious for capital breeding carnivores, such as marine mammals, which rely on lipid stores accrued during intensive foraging to sustain prolonged periods of fasting associated with reproduction. Therefore, chronic stress may cause a decrease in fitness in these animals, leading to population declines and potentially detrimental shifts in food web dynamics as a result. However, the impacts and indicators of chronic stress in animals are currently poorly understood. To identify downstream mediators of repeated stress responses in marine mammals, adrenocorticotropic hormone …

Sexual Dimorphism In Aortic Function Of Uc Davis Type 2 Diabetes Mellitus Rat Model: Estrogen Specific Responses, Farjana Akther

University of the Pacific Theses and Dissertations

Little is known about the interaction between diabetes and sex in vasculature. This study was designed to investigate the effects of estrogen as well as type 2 diabetes (T2D) on aortic function in rats with respect to sex. To test the effects of T2D and sex, UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) rat model was used. To study the effects of estrogen, ovariectomized Sprague- Dawley female rats and UCD-T2DM rats at pre-diabetic stage were used and the rats were implanted subcutaneously either with placebo or 17 β-estradiol pellets (60 days release, 1.5mg/pellets). The plasma analytes for metabolic parameters and …

Phylogenomic Analyses Clarify Butterfly Species Within The Genus Speyeria Despite Evidence Of A Recent Adaptive Radiation, Erin Thompson

University of the Pacific Theses and Dissertations

The North American genus Speyeria is an especially challenging radiation of butterflies due to ongoing hybridization, incomplete lineage sorting, and similar morphological characters among species. Adaptive radiations often require considerable evidence in order to resolve the evolutionary relationships of closely related individuals. Previous studies of this genus have found paraphyly among species and have been unable to disentangle these taxa due to a lack of data and/or incomplete sampling of the genus. As a result, the interspecific relationships among Speyeria remain unresolved. In an attempt to achieve phylogenetic resolution of the genus, we conducted population genomic and phylogenomic analyses of …

Improving Teacher Retention By Addressing Teachers' Compassion Fatigue, Jacquelyn Ollison

University of the Pacific Theses and Dissertations

California is experiencing a massive teacher shortage, and urban schools are disproportionately affected by it negatively. Retention efforts to date have not included strategies to address Compassion Fatigue (burnout and secondary trauma) teachers experience when working with traumatized students at urban schools. This dissertation explores whether Compassion Fatigue is an unaddressed reason for teacher attrition at urban schools. A mixed method practical action research approach using the Professional Quality of Life Scale Version Five (ProQOL 5) and qualitative interviews, portions of which were turned into illustrative vignettes drove the exploration. Approximately 114 teachers completed the ProQOL 5. Statistical analysis of …

Exercise And Patient Populations: A Community-Based Exercise Intervention On Cancer And Type 2 Diabetes, Cynthia Villalobos

University of the Pacific Theses and Dissertations

Cancer and diabetes are the second and seventh leading causes of death in the United States, respectively (Kochanek et al., 2017). When including prediabetes and the entire cancer continuum, both diseases affect over 150 million individuals in the United States each year (Bullard et al., 2018; Siegel et al., 2019). Furthermore, there exists an alarming rise in patients presenting with both type 2 diabetes and obesity-related cancers concurrently, as both diseases share similar risk factors (Vigneri et al., 2009). An aging adult population, physical inactivity and unhealthy eating habits are continuing to rise, and the prevalence of individuals with obesity-related …

The Effects Of Design Thinking On Students' Career Self-Efficacy In Career Guidance Courses, Zhongmiao Sun

University of the Pacific Theses and Dissertations

The present study focuses on integrating design thinking into career guidance courses to test whether students’ career self-efficacy is increased by comparing the experiment group (by using design thinking method) and the general group (by using traditional teacher-centered method). The basic theoretic framework is Bandura’s self-efficacy theory (Bandura, 1977). Students will achieve career self-efficacy after experiencing repeatedly success (Bandura, 1977) in the career activities through design thinking method. Then students will have more confidence to make more appropriate career choices in their employment environment.

This study used AMOS and path analysis to analyze a just-identified model. The model included five …

Developing Collective Teacher Efficacy In One Urban Low-Income Elementary School: A Case Study, Lori Ann Morgan

University of the Pacific Theses and Dissertations

Over the past two decades, research has shown links between collective teacher efficacy and student achievement. While the benefits of high levels of collective teacher efficacy have been documented, research focused on how it is developed in school serving socio-economically disadvantaged students and the role of principal leadership in that development is lacking, specifically from a qualitative case-study approach This qualitative case-study explored how collective teacher efficacy was developed in an urban neighborhood elementary school serving socio-economically disadvantaged students and how the principal’s leadership influenced that development. This was accomplished through in-depth individual interviews with teachers, support staff, and the …

The Relationship Between The Cefi, As A Measure For Executive Function, And The Basc-3, As A Measure Of Externalizing Behavior, On School Problems For Children Receiving Special Education Services, David Looney

University of the Pacific Theses and Dissertations

This study examined the relationship between executive function and externalizing behaviors within a student population that was assessed for special education services. Executive function was measured by using the Comprehensive Executive Function Inventory (CEFI). Externalizing behaviors and school problems was measured using the Behavior Assessment System for Children, Third Edition (BASC-3). Four separate structural equation models were produced and analyzed to examine this relationship. The results from this study indicate that there is a significant direct effect from executive function on externalizing behaviors and school problems, such that higher scores in executive function yielded lower externalizing behavior and school problems …

Effects Of Extended Intervention Conditions On Levels Of Physical Activity Exhibited By Young Children, Ingunn Kristjansdottir Oveny

University of the Pacific Theses and Dissertations

Physical activity is an important health-related behavior, and The Centers for Disease Control and Prevention (CDC) recommends that children engage in at least 60 minutes of moderate-to-vigorous-physical activity (MVPA) daily (CDC, 2015). However, worldwide, many children do not reach those requirements and health problems associated with physical inactivity are becoming more prevalent (CDC, 2015; World Health Organization [WHO], 2016). Recently, a few studies have conducted an intervention analysis to evaluate implications for function-based interventions to increase physical activity (Larson, Normand, Morley, & Miller, 2014; Zerger, Normand, Boga, & Patel, 2016). However, intervention analyses, indicate an overall decrease in levels of …

Transcriptome Analysis For Novel Peptide In Breeding Gland Of Hymenochirus Boettgeri, Minjin Ko

University of the Pacific Theses and Dissertations

The primary goal of the project was to find nucleotide sequences potentially encoding a pheromone from the breeding gland of Hymenochirus boettgeri. The reasons in searching for the sequence of a pheromone were to better understand the organism and to use the information for application in reproduction of other species. Due to climate change and rampant deforestation, such as in Africa’s Congo Basin, many amphibian species are being threatened. With these increasing threats, a viable option for the future may be breeding in captivity for the amphibian species. Pheromone characterization from the breeding gland of Hymenochirus boettgeri may help with …

Characterization Of The Roles Of Ppp1r15a (Gadd34) And Ppp1r15b (Crep) In Er Stress-Induced Apoptosis In Vivo, Danielle Hicks

University of the Pacific Theses and Dissertations

Failure to balance synthesis, folding and degradation of secreted proteins results in the accumulation of unfolded proteins in the endoplasmic reticulum, termed ER stress. Cells respond to ER stress by activating a signaling pathway known as the unfolded protein response (UPR). The UPR induces phosphorylation of eIF2α to attenuate global protein translation, allowing the ER to clear misfolded proteins. Opposing this function, eIF2α phosphatases contain a catalytic subunit, Protein Phosphatase 1, and either of two homologous scaffolding subunits, GADD34 and CReP. Inhibition of eIF2α phosphatases has been shown to prolong UPR signaling and promote survival in many cells types and …

The Associations Between The Perception Of Helpfulness Of Teacher Induction Programs, Teacher Self-Efficacy, And Anticipated First-Year Teacher Retention In Shanghai Public Primary Schools, Xiaotian Han

University of the Pacific Theses and Dissertations

The purpose of the study was to: (a) determine to what extent the formalized teacher induction programs (TIPs) in Shanghai are perceived to be helpful for first-year public primary school teachers; (b) measure teacher self-efficacy and anticipated job retention of first-year teachers in Shanghai public primary schools; and (c) examine the degree to which these perceptions of helpfulness, teacher self-efficacy, and anticipated job retention are associated. In this study, retention is defined as remaining in a public primary school in Shanghai. Shanghai TIPs are one-year long, mandatory programs for first-year teachers in Shanghai public primary schools. The conceptual framework of …

A Trio Of Sigma Factors Control Hormogonium Development In Nostoc Punctiforme, Alfonso Gonzalez Jr.

University of the Pacific Theses and Dissertations

Cyanobacteria are prokaryotes capable of oxygenic photosynthesis, and for many species, nitrogen fixation, giving cyanobacteria an important role in global carbon and nitrogen cycles. Furthermore, multicellular filamentous cyanobacteria are developmentally complex, capable of differentiation into different cell types, including cells capable of nitrogen fixation and cells for motility, making them an ideal platform for studying development, as well as for practical use in biotechnology. Understanding how developmental programmes are activated require an understanding of the role of alternative sigma factors, which are required for transcriptional activation in bacteria. In order to investigate the gene regulatory network and to determine the …

Roaming Transition States And Highly Accurate Thermochemistry: A Pepico Study Of Two Small Combustion Systems, Kyle John Covert

University of the Pacific Theses and Dissertations

Two small combustion systems, methyl hydroperoxide (CH₃OOH) and 2-propanol ((CH₃)₂CHOH), were studied using imaging photoelectron photoion coincidence spectroscopy (iPEPICO), which combines photoelectron spectroscopy and photoionization mass spectrometry to detect coincident photoelectron-photoion pairs. In the photon energy range of 11.4–14.0 eV, energy selected CH₃OOH⁺ ions dissociate into CH₂OOH⁺, HCO⁺, CH₃⁺, and H₃O⁺ ions. The lowest-energy dissociation channel is the formation of the cation of the smallest “QOOH” radical, CH₂OOH⁺. A statistical rate model fitted to the …

Educational Success Of Low-Income And First Generation College Students In Private Colleges Of China, Yinghui Chen

University of the Pacific Theses and Dissertations

In China, low-income and first generation college students in private institutions are faced with adversities which undermine their educational outcome. Since the previous researches are mainly deficit-focused, the researcher conducted an investigation which put emphasis on the students’ successful educational outcome in order to round out the knowledge of this problem. The researcher employed a qualitative multiple case study to explore how three students accomplished educational resilience despite their hardships. The researcher findings suggested that: 1) Internal locus of control, career and academic planning, financial aids, job-related experience, and rational occupational aspiration contribute to students’ educational success; 2) The primary …

Student Perceptions Of The Clip Chart Management System, Karin D. Compise

University of the Pacific Theses and Dissertations

Six children between the ages of seven and eleven and their parents were interviewed to gather perceptions of the Clip Chart Management System. The Clip Chart system is a behavior system used in many primary classrooms where teachers move students’ clothespins up and down a chart in response to students’ behavior. Some findings from this qualitative case study are: students experienced feelings of shame and embarrassment, students compared their clips to their peers’ clips, and students labeled other students as “bad.” Some parents appreciated the consistency of behavior monitoring, but other parents felt that the system was ineffective and contributed …

Development And Characterization Of Ldv Peptide Targeted Nanocarriers For Paclitaxel Delivery: A Comparative Study Of Micelles, Liposomes And Solid Lipid Nanoparticles, Poonam Dattani

University of the Pacific Theses and Dissertations

Nanocarriers have been established as delivery vehicles to target cancer tumors. However, premature drug leakage is one of the major reasons for inefficient drug delivery of nanocarriers to the tumor. Drug diffusion out of the nanocarriers or destabilization of drug loaded nanocarriers by physiological interactions with blood cells, serum proteins, and cell membranes upon systemic administration contribute to premature drug release. In this study, targeted micelles, liposomes and solid lipid nanoparticles (SLNs) of similar composition were prepared and characterized to compare physicochemical characteristics, in vitro stability, in vitro release rates in release media and in vivo performance. Peptide Amphiphiles (PAs) …

The Impact Of Social And Cognitive Variables On Communication Competence, Griffin Cheek

University of the Pacific Theses and Dissertations

College students are increasingly engaged in watching online videos and using social media. Therefore, researchers should attempt to better understand how these variables as well as other social and cognitive variables, affect the communication competence of students. The researcher administered a questionnaire to 392 college students from a private medium-sized West Coast university using various modified scales examining student-to-student confirmation, social support through social media, online video viewing, self-esteem, personal reports of public speaking anxiety, and communication competence. Student-to-student confirmation and online video viewing were measured using new sets of scales developed by the researcher. The data from the surveys …

Poison Ivy's Green Screen Debut: A Rhetorical Criticism On Erasing Identity On Screen, Jennifer Baney

University of the Pacific Theses and Dissertations

This project investigates the loss of power on screen for female comic book characters. Specifically, I investigate how scenes create narratives using heteronormativity and over-sexualization of female characters. The artifact of analysis included in this project is Batman and Robin (1997). This text focuses on Poison Ivy, including the background of the character before dissecting her role in the film. Turning to Sonja J. Foss (2009) and her feminist critique as a guide to understanding the implications of this research. Using feminist criticism, I argue that Poison Ivy was put in a lesser position, removed of her power, and was …

An Evaluation Of Individual And Group Behavioral Skills Training To Teach Members Of Greek Letter Organizations To Free-Pour Standards Of Alcohol, Meagan Anne Strickland

University of the Pacific Theses and Dissertations

College students engage in high levels of excessive drinking and certain subpopulations of college students, such as members of Greek Letter Organizations (GLOs) report higher levels of excessive alcohol consumptions. Those who report less excessive drinking also report counting their drinks and setting drink limits. However, the effectiveness of these strategies may be improved by the ability to accurately identify and pour standard servings of alcohol, an area in which college students’ knowledge is generally quite poor. Although individual behavioral skills training (BST) has been used to teach college students to accurately pour beer (Hankla et al., 2017), little is …

The Effects Of Mindfulness Practice With Music Listening On Working Memory, Emily Irene Messick

University of the Pacific Theses and Dissertations

The purpose of this experimental study was to investigate mindfulness strategies and their influence on working memory. The potential role of music in facilitating mindfulness practice is explored. Various listening exercises were investigated along with their influence on working memory (i.e. attention control). Thirty-four individuals were randomly assigned to participate in one of four listening groups: 1) mindfulness with music, 2) mindfulness without music, 3) music only, and 4) silence. Thirty-four participants engaged in a computerized digit-span task before and after the listening exercise to assess pre- and post-test working memory performance. Thirty participants were included in data-analysis due to …

The Effect Of Energy Drinks On Cardiovascular Variables: A Randomized Controlled Trial, Cynthia Lee

University of the Pacific Theses and Dissertations

Many studies have investigated the effects of energy drinks on cardiovascular parameters. These studies were typically conducted with high volume (32 ounces) energy drinks and have demonstrated association with QT prolongation and blood pressure elevation after consumption. Currently, there is inconclusive evidence with lower volume energy drinks. This study intends to evaluate the effects of the largest commercially available energy drink can (24 ounces) in the United States on cardiovascular parameters compared with placebo arm. A randomized, double-blinded, placebo-controlled, crossover trial was conducted over 2 separate days with a minimum of 6-day wash out period. Healthy volunteers between the age …

Knob-Socket Investigation Of Stability And Specificity In Alpha-Helical Secondary And Quaternary Packing Structure, Taylor Renee Rabara

University of the Pacific Theses and Dissertations

The novel knob-socket (KS) model provides a construct to interpret and analyze the direct contributions of amino acid residues to the stability in α-helical protein structures. Based on residue preferences derived from a set of protein structures, the KS construct characterizes intra- and inter-helical packing into regular patterns of simple motifs. The KS model was used in the de novo design of an α-helical homodimer, KSα1.1. Using site-directed mutagenesis, KSα1.1 point mutants were designed to selectively increase and decrease stability by relating KS propensities with changes to α-helical structure. This study suggests that the sockets from the KS Model can …

Oral Health Care: An Autoethnography Reflecting On Dentistry's Collective Neglect And Changes In Professional Education Resulting In The Dental Hygienist Being The Prevention-Focused Primary Oral Health Care Provider, Maureen Patricia Mcweeney Harrington

University of the Pacific Theses and Dissertations

Many factors influence poor oral health among disadvantaged populations including socioeconomic circumstances, knowledge of disease prevention strategies and ability to implement those strategies, public policies, insurance status, insurance policies, dental providers and other challenges to accessing dental care. Often these issues converge and result in early disadvantages to achieving good oral health (Horton & Barker, 2010). Addressing even some of the factors that contribute to poor oral health may provide ways to change the dental health status of historically underserved populations. The purpose of this research is to explore my role as a practitioner and researcher in the creation of …

How A Small Business Negotiates Digital Inclusion Of People With Disabilities: A Case Study, Belo Miguel Cipriani

University of the Pacific Theses and Dissertations

Technology has provided more people access products and services, yet some individuals who would benefit the most from digital access to resources are frequently excluded from participation. One group that is largely neglected is the disability community. Despite federal regulations intended to ensure that people with physical/mental disabilities are included in public digital platforms, organizations continuously design websites, applications, and interfaces without people with disabilities in mind. This is particularly the case with small businesses, which are most commonly reported as having inaccessible digital platforms.

Digital inclusion attempts to ensure equity in digital properties by providing a model to operationalize …

Approaches To The Search Of Platinum Anticancer Agents: Derivatizing Current Drugs And Incorporating Hdac Inhibition, Chao Feng

University of the Pacific Theses and Dissertations

Platinum-based anticancer drugs, such as cisplatin, carboplatin, and oxaliplatin, have been approved for clinical use worldwide for decades. Despite their enormous success, their widespread application is hindered by either cross-resistance or toxic side effects, including nephrotoxicity and neurotoxicity. The need to overcome these drawbacks has stimulated the search for new platinum-based drugs.

This dissertation will start with the accidental discovery of cisplatin, followed by an introduction of other platinum-based anticancer agents, including the action mechanism, general structures, and development history. Picoplatin is a newer generation of platinum-based anticancer agent. The bulky 2-methylpyridine as a non-leaving group on picoplatin could reduce …

Exploring The Molecular Origin Of Jet Fuel Thermal Oxidative Deposition Through Statistical Analysis Of Mass Spectral Data And Pyrolysis Gas Chromatography/Mass Spectrometry Of Deposits, Krege Matthew Christison

University of the Pacific Theses and Dissertations

ASTM D3241 (Standard Test Method for Thermal Oxidation Stability of Aviation Turbine Fuels) measures the thermal oxidative stability of jet fuels under elevated temperature and pressure conditions. When jet fuels fail ASTM D3241, either at the refinery or in the distribution system, there can be supply disruptions and financial losses. Understanding the causes of poor thermal oxidative stability in jet fuels could help prevent or mitigate issues. In order to develop a deeper understanding of the molecular precursors that lead to ASTM D3241 failures, a number of analytical methodologies and data treatment techniques have been developed, applied, and reported here. …

Investigating The Effect Of Rutaecarpine On The Benzo[A]Pyrene-Induced Dna Damage In Vitro, You Li

University of the Pacific Theses and Dissertations

Benzo[a]pyrene (BaP), is one of the most potent mutagens and carcinogens known. It requires metabolic activation through cytochrome P450 (CYP)1A1 to yield the ultimate carcinogenic metabolite, benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE). BPDE can bind to DNA and form predominantly covalent (+) trans adducts at the N2 position of guanine causing DNA damage. Rutaecarpine (RTC) is an herbal medicine that has been used to treat several diseases such as headache, hypertension, gastrointestinal disorders, amenorrhea, and anti-inflammation. It has also been reported as a potent inducer of CYP enzymes, including CYP1A1, and CYP1A2. The mechanisms underlying up-regulation of CYP1A1 by RTC is dependent on aryl …

Teachers’ Self-Efficacy Beliefs In Relation To Perceived English Proficiency And Teaching Practices: An Investigation Of Chinese Primary English As A Foreign Language (Efl) Teachers, Yun Zhang

University of the Pacific Theses and Dissertations

Research on self-efficacy has been a productive field and abundant research has shown that teachers’ self-efficacy beliefs influence teachers’ actions and performances and thus affect students’ learning outcomes. However, there is a lack of literature on EFL teachers’ self-efficacy beliefs and even less research was set in Chinese EFL contexts. On the one hand, this study was conducted to provide a general picture of the current status of EFL teachers’ perceived English proficiency, self-rated self-efficacy beliefs and self-reported teaching practices in terms of some demographic perspectives; On the other hand, it aimed to explore the correlations among Chinese primary EFL …