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2019

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Use Of Digital Panoramic Radiographs As A Screening Tool For Diagnosis Of Osteoporosis In Thai Postmenopausal Women, Net-Nada Chongruangsri Jan 2019

Use Of Digital Panoramic Radiographs As A Screening Tool For Diagnosis Of Osteoporosis In Thai Postmenopausal Women, Net-Nada Chongruangsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose: To investigate the correlation between panoramic radiographic indices and osteoporosis, and to determine whether digital panoramic radiographs could be used as a screening tool for the diagnosis of osteoporosis in Thai postmenopausal women. Materials and Methods: A cross-sectional study of sixty Thai postmenopausal women with and without osteoporosis. The subjects were divided into three groups based on diagnosis by dual-energy X-ray absorptiometry (DXA): normal, osteopenia, and osteoporosis equally in each group. The panoramic radiographic indices measured were mental index (MI) and mandibular cortical index (MCI). Pearson’s correlation test was performed to analyze any correlation between MI, MCI, and BMD …

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Association Between Clinical Signs And Quality Of Life In Thai Patients With Oral Lichen Planus, Sasirin Yiemstan Jan 2019

Association Between Clinical Signs And Quality Of Life In Thai Patients With Oral Lichen Planus, Sasirin Yiemstan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Subjective patient’s symptoms and oral health-related quality of life (OHRQoL) were recommended to be involved in oral lichen planus (OLP) studies. Objectives: to evaluate 1) the association between OLP clinical signs and OHRQoL as well as pain perception. 2) the association between OLP pain and OHRQoL. Methods: Sixty-nine OLP or oral lichenoid drug reaction (OLDR) Thai patients were recruited. Data were collected through personal interview with the Numeric Rating Scale (NRS) and Thai version of Oral Impacts on Daily Performance (OIDP) index. OLP signs were examined in aspects of localization, type and clinical severity according to the Thongprasom sign scoring …

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Clinical Evaluation Of Two Glass Ionomer Cement Restorations Placed In Primary Molars With Atraumatic Restorative Treatment Technique: A Randomized Controlled Trial., Manarin Boonyawong Jan 2019

Clinical Evaluation Of Two Glass Ionomer Cement Restorations Placed In Primary Molars With Atraumatic Restorative Treatment Technique: A Randomized Controlled Trial., Manarin Boonyawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: The purpose of this study was to compare clinical success of a novel material (silver diamine fluoride incorporated with high-viscosity glass ionomer cement (SDF-GIC)) in Class II restoration in primary molars using atraumatic restorative treatment (ART) technique at 6- and 12- month follow-up. Materials and Methods: A randomized controlled clinical trial using a parallel group design was carried out on 150 children aged 3-8 years old, from 5 public school in Samut Sakhon province, Thailand, with at least one class II cavities. They were randomly allocated to two treatment groups: ART restoration using either GIC (Fuji IX GP) or …

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Protein Expression And Inflammatory Response After Delivery Of Mrna Encoding Platelet-Derived Growth Factor-Bb In Sucrose Citrate Buffer Into Rat Gingiva, Phan Bhongsatiern Jan 2019

Protein Expression And Inflammatory Response After Delivery Of Mrna Encoding Platelet-Derived Growth Factor-Bb In Sucrose Citrate Buffer Into Rat Gingiva, Phan Bhongsatiern

Chulalongkorn University Theses and Dissertations (Chula ETD)

Messenger RNA (mRNA) has emerged as a novel therapeutic modality in medical fields, including regenerative medicine. The concept of mRNA-based therapy is to use synthesized mRNA encoding therapeutic protein delivered to target tissue. Thus, mRNA encoding growth factor could be a promising alternative to recombinant protein. Platelet-derived growth factor (PDGF) is one of most extensively studied growth factors for periodontal regeneration. To date, mRNA therapy has never been explored in the field of periodontal regeneration. The aim of the study is to examine the effect of nucleoside-modified mRNA encoding PDGF-BB on the level of PDGF-BB protein and inflammatory response at …

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The Use Of Modified Mrna Encoding Platelet-Derived Growth Factor-Bb As An Innovation In Periodontal Regeneration, Theeraphat Surisaeng Jan 2019

The Use Of Modified Mrna Encoding Platelet-Derived Growth Factor-Bb As An Innovation In Periodontal Regeneration, Theeraphat Surisaeng

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since the mRNA platform has been recently introduced to several fields in medicine and the achievement of periodontal regeneration is currently unpredictable, therefore, the use of mRNA technology is a promising approach to pursue the complete periodontal regeneration. The aims of this study are to learn if mRNA encoding platelet-derived growth factor-BB (PDGF-BB) induces PDGF production in human periodontal ligament cells (PDLCs) and to investigate the effect of secreted PDGF on PDLC proliferation and promoting endothelial cell tube formation. PDLCs were obtained from extracted teeth of healthy periodontal patients. The N1-methylpseudouridine modified mRNA encoding PDGF-BB were transfected into PDLCs. The …

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Production Of Bio-Jet Fuel From Palm Fatty Acid Distillate Over Bi-Functional Copd/Hzsm-12 Catalysts, Jarujit Chaowichitra Jan 2019

Production Of Bio-Jet Fuel From Palm Fatty Acid Distillate Over Bi-Functional Copd/Hzsm-12 Catalysts, Jarujit Chaowichitra

Chulalongkorn University Theses and Dissertations (Chula ETD)

Jet fuel is one of the major products from a petroleum refinery, its combustion emissions contribute to global warming. Therefore, bio-jet fuel is introduced to solve this problem. Palm fatty acid distillate (PFAD) is an interesting feedstock for bio-jet fuel production via three reactions including deoxygenation, hydrocracking, and isomerization. In this study, the monometallic (Co and Pd) and bimetallic (CoPd and NiPd) supported on HZSM-12 zeolite with various metal loadings and bimetallic CoPd with various zeolite supports (HZSM-12, HZSM-5, and HY) were investigated in a continuous flow trickle bed reactor. The presence of bimetallic CoPd on HZSM-12 zeolite support enhanced …

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Removal Of Trace Olefins From Aromatics With Modified Usy Zeolite And Clays, Sirawit Lertprapaporn Jan 2019

Removal Of Trace Olefins From Aromatics With Modified Usy Zeolite And Clays, Sirawit Lertprapaporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Naphtha reforming is an important raw material for aromatic hydrocarbon products in petrochemical processes. However, aromatic hydrocarbon streams obtained from refinery contain unavoidable trace amounts of olefins as an impurity. The trace olefins must be removed because these undesirable olefins are harmful to the following separation processes. Alkylation reaction has been used to remove olefins with acid catalysts. In this study, clays and zeolites were screened for further modification in order to enhance the catalytic activity of olefins removal. The catalytic activity testing was studied in a batch reactor at 195 ℃ for 6 h under 12 barg. USY was …

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Combined Life Cycle Analysis And Material Flow Analysis As A Tool For End Of Life Options Of Used Lubricating Oils, Komsan Toeipomthong Jan 2019

Combined Life Cycle Analysis And Material Flow Analysis As A Tool For End Of Life Options Of Used Lubricating Oils, Komsan Toeipomthong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Used lubricating oils are one of the most significant material flows in the economy. After being used, they are typically contaminated with, e.g., heavy metals, which could harm the environments if they are treated improperly. The used oils should be properly treated by reprocessing or recycling which the recyled products can be used to subsitute the production the new product, as a result a net reduction of the environemental impacts. This study aimed to investigate the waste flow and then evaluate the environmental impacts of waste management options using the Material Flow Analysis (MFA, STAN v.2.6.801) and Life Cycle Assessment …

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Continuous Synthesis Of Biodiesel And Glycerol Ether Using Homogeneous And Heterogeneous Catalysts, Natta Rattanapanya Jan 2019

Continuous Synthesis Of Biodiesel And Glycerol Ether Using Homogeneous And Heterogeneous Catalysts, Natta Rattanapanya

Chulalongkorn University Theses and Dissertations (Chula ETD)

In our study, a mixture of p-toluenesulfonic acid and sulfuric acid (TsOH-H2SO4) as a catalyst showed a good performance in transesterification of palm oil (PO) with methanol and etherification of biodiesel with isobutylene. For biodiesel production, the catalyst noticeably accelerated the reaction faster than TsOH and H2SO4 alone and also gave up to 99.8% of the conversion. Etherification of biodiesel with isobutylene in the etherification process using isobutylene/glycerol molar ratio 6:1 at 70°C, in the period of 5 h reaction time, gave high selectivity to produce DTBG and TTBG (81.4%). Furthermore, the catalyst can be reused for 6 cycles of …

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Formaldehyde Fluorescent Sensors From 1,8-Naphthalimide Derivatives, Apicha Maharat Jan 2019

Formaldehyde Fluorescent Sensors From 1,8-Naphthalimide Derivatives, Apicha Maharat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Three derivatives of 1,8-naphthalimide were systematically designed and synthesized to investigate the effect of substitution pattern on their photophysical properties and sensing behaviors towards formaldehyde in aqueous media. The key substituents, the hydrazino (-NHNH2) and the 2-methoxyethylamino groups (-NHCH2CH2OCH3), were installed at the 4-position of 1,8-naphthalimide by a nucleophilic replacement, and at the imide position by a condensation reaction with 1,8-naphthalic anhydride precursor. All target compounds were obtained in good overall yields of 60-80%. Compounds with two hydrazine moieties (R3) showed excellent selective fluorescent responses towards formaldehyde with the 3.5-fold fluorescence enhancement in 5% aqueous acetic acid solution at 520 …

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Polyvinyl Alcohol/Starch Modified Cotton Thread For Glucose Distance-Based Colorimetric Detection, Pornchanok Punnoy Jan 2019

Polyvinyl Alcohol/Starch Modified Cotton Thread For Glucose Distance-Based Colorimetric Detection, Pornchanok Punnoy

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this thesis, polyvinyl alcohol (PVA) and starch modified cotton thread was developed as a cotton thread-based device for distance-based colorimetric detection of hydrogen peroxide (H2O2) and glucose. PVA and starch, the biocompatible polymers, were modified on a cotton thread to enhance the enzymatic stability and reagent immobilization efficiency. The colorimetric glucose detection of the device was based on a bienzymatic reaction involving glucose oxidase (GOx) and horseradish peroxidase (HRP), incorporated with potassium iodide (KI) as an indicator, which was oxidized by H2O2 to provide blue-black color band of an iodine–starch complex. The length of color band on the cotton …

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Continuous Flow Selective Hydrogenation Of 5-Hydroxymethylfurfural To 2.5-Dimethylfuran Using Pd-Cu/Reduced Graphene Oxde Catalysts, Sareena Mhadmhan Jan 2019

Continuous Flow Selective Hydrogenation Of 5-Hydroxymethylfurfural To 2.5-Dimethylfuran Using Pd-Cu/Reduced Graphene Oxde Catalysts, Sareena Mhadmhan

Chulalongkorn University Theses and Dissertations (Chula ETD)

2,5-Dimethylfuran (DMF) has been considered a promising biofuel, potentially derived from biomass. There have been various reports on DMF production from hydrogenation of 5-hydroxymethylfurfural (HMF). However, most reports employed high hydrogen pressure, long reaction times, and reactions under batch reactor. In this study, Pd-Cu bimetallic catalysts incorporated on reduced graphene oxide (RGO) were used for hydrogenation of HMF to DMF using 2-propanol as hydrogen donor under continuous flow system. Synthesized catalysts were characterized by N2 physisorption, scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and temperature programmed reduction of hydrogen …

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Preparation Of Starch-Filled Polyisocyanurate Foams Catalyzed By Zinc-Ammonia Complex/Potassium Octoate, Sirima Goonack Jan 2019

Preparation Of Starch-Filled Polyisocyanurate Foams Catalyzed By Zinc-Ammonia Complex/Potassium Octoate, Sirima Goonack

Chulalongkorn University Theses and Dissertations (Chula ETD)

The research aimed to reduce apparent density and improve fire resistance of polyisocyanurate (PIR) foam. PIR foams were prepared using zinc-ammonia complex and potassium octoate as catalyst for gelling/blowing and trimerization reactions, respectively. Three types of starch produced in Thailand, namely glutinous rice flour, rice flour and mung bean starch, were used as additives for PIR foams. The characterized properties of PIR foams modified with starches are reaction times (cream time, gel time, rise time and tack free time), polyisocyanurate/polyurethane (PIR/PUR) ratio, %isocyanate conversion, apparent density, compressive property, morphology and fire behavior. These properties are compared with PIR foam without …

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Magnetic Nanoparticles Stabilized By Phosphorylcholine-Containing Polymer For Antibody Free C-Reactive Protein Detection, Suttawan Saipia Jan 2019

Magnetic Nanoparticles Stabilized By Phosphorylcholine-Containing Polymer For Antibody Free C-Reactive Protein Detection, Suttawan Saipia

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aims to develop a simple, yet effective assay for C-reactive protein (CRP) detection based on a combination of magnetic separation and antibody-free colorimetric assay. Magnetic nanoparticles stabilized with phosphorylcholine-containing polymer, poly[methacrylic acid)-ran-(methacryloyloxyethyl phosphorylcholine)] (PMAMPC-MNPs) were prepared by co-precipitation of ferric and ferrous salts in the presence of PMAMPC. Carboxyl groups in the methacrylic acid (MA) repeat units chelate with Fe atoms during MNPs formation while the methacryloyloxyethyl phosphorylcholine (MPC) repeat units provide specifically binding sites and conjugate with CRP in presence of Ca2+. The PMAMPC-MNPs were characterized by ATR-FTIR, TEM, DLS, TGA and XRD. To determine the CRP …

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Electrospun Nylon 6,6 Nanofibers Functionalized With Β-Cyclodextrin As Solid Phase Extraction Sorbent For Determination Of Parabens, Tassanee Kuakul Jan 2019

Electrospun Nylon 6,6 Nanofibers Functionalized With Β-Cyclodextrin As Solid Phase Extraction Sorbent For Determination Of Parabens, Tassanee Kuakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, electrospun nanofibers of nylon 6,6 functionalized with β-cyclodextrin (nylon 6,6/β-CD) were fabricated and applied as a sorbent in solid-phase extraction (SPE) for determination of parabens in water. The electrospinning parameters such as polymer concentration, nylon 6,6/β-CD ratio, and electric field strength were investigated in order to obtain uniform nanofibers. The morphology and physical properties of obtained fibers were characterized by scanning electron microscope (SEM), Fourier transforms infrared spectroscopy (FTIR), and BET surface area analysis. The electrospun nylon 6,6/β-CD nanofibers were achieved with diameter in the range of 120 - 150 nm. The additional of β-CD slightly increased …

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The Production Of Bio-Jet Fuel From Palm Fatty Acid Distiliate Over Covalt And Nickel Supported Hzsm-12 Catalysts, Ariya Eka Alel Jan 2019

The Production Of Bio-Jet Fuel From Palm Fatty Acid Distiliate Over Covalt And Nickel Supported Hzsm-12 Catalysts, Ariya Eka Alel

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aviation sector becomes one of the largest-developing public transportation nowadays, the number of air passangers always increases year by year. However, this number are proportionally related to the enhance of jet-fuel worldwide demand. The conventional jet-fuel are commonly known as a carbon dioxde emission source and the world has more concern to this pollutant due to its contribution to the climate change. The usage of renewable materials to produce jet fuel is more glanced. One of the renewable sources can be used to generate green jet fuel is palm fatty acid distillate (PFAD) which is a by-product of palm oil …

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High Magnification Polymeric Lens For Smartphone Microscope, Wisansaya Jaikeandee Jan 2019

High Magnification Polymeric Lens For Smartphone Microscope, Wisansaya Jaikeandee

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this work, we present a facile method for fabrication of plano-convex lenses, using high reflective optical polymer (Polydimethylsiloxane, Sylgard 184 by Dow Corning, n ~1.42 and Norland Optical adhesive 61, NOA61 by Norland Products, ~1.56) under the confined sessile drop technique. The confined sessile drop technique is a facile method and an adjustable lens geometry through controlled the weight of liquid polymer on the lens substrate, as PMMA circular disk and Sylgard circular disk, with different diameter (2.5-6.0 mm). The liquid polymer was gradually spread and radially over the surface of the lens substrate, and resistance to spreading of …

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Natural Gas Storage Using Clathrate Technology : Enhanced Formation At High Temperature, Kan Jeenmuang Jan 2019

Natural Gas Storage Using Clathrate Technology : Enhanced Formation At High Temperature, Kan Jeenmuang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Solidified natural gas (SNG) by storing natural gas in the hydrate form, which is safe and easy to recover stored gas compared to other methods. However, to make this technology competitive, storing natural gas at a higher temperature, lower pressure with faster formation is needed. Tetrahydrofuran (THF) is a well-known thermodynamic promoter used to increase the hydrate formation temperature. However, increasing the hydrate formation temperature has a drawback on decreasing the formation rate. Nonpolar amino acids have been used as a secondary promoter, which increases the hydrate formation kinetics. This work used 5.56 mol% THF cooperated with different concentrations of …

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Stochastic Supply Chain Optimization For Ammonia And Urea Processes, Seangplang Srisamut Jan 2019

Stochastic Supply Chain Optimization For Ammonia And Urea Processes, Seangplang Srisamut

Chulalongkorn University Theses and Dissertations (Chula ETD)

To support the demand growth in the agriculture sector from increasing population, ammonia and urea productions have been continuously increasing. Methane from natural gas can be used as feedstock in the production process. Nevertheless, the conventional plant using natural gas feedstock produces carbon dioxide as a by-product of the process. This proposed plant contains two processes, producing ammonia and urea can be more efficient in the production, where the byproducts of CO₂ can be used to produce more urea and reduce CO₂ emission. This research proposes ammonia and urea synthesis process using PROII software to simulate workflow and estimate the …

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Effects Of Sulodexide In The Prevention Of Peritoneal Membrane Changes In Continuous Ambulatory Peritoneal Dialysis Patients, Panida Ditsawanon Jan 2019

Effects Of Sulodexide In The Prevention Of Peritoneal Membrane Changes In Continuous Ambulatory Peritoneal Dialysis Patients, Panida Ditsawanon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this placebo-controlled clinical study is to determine the effect of sulodexide for the prevention of peritoneal membrane change in PD patients by evaluating dialysate biomarkers of peritoneal membrane change, phenotypes of peritoneal mesothelial cells, peritoneal membrane transports and safety. A total of 66 patients were included in this randomized control trial study. Patients were randomly assigned to receive either 50 mg of sulodexide or placebo by oral 2 times daily. PET was performed to evaluate peritoneal transport function. Dialysate CA125, IL-6 and VEGF concentration were also measured at baseline and after treatment by ELISA. Peritoneal mesothelial cell …

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Taxonomy, Bioactivities, And Secondary Metabolites Of Endophytic Actinomycetes Isolated From Medicinal Plants, Nattaporn Klykleung Jan 2019

Taxonomy, Bioactivities, And Secondary Metabolites Of Endophytic Actinomycetes Isolated From Medicinal Plants, Nattaporn Klykleung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fifty endophytic actinomycetes isolated from six medicinal plants in Thailand were identified as Micromonospora (34 strains), Streptomyces (8 strains), Microbispora (3 strains), Nonomuraea (2 strains), Plantactinospora (2 strains), and Amycolatopsis (1 strain) based on phenotypic characteristics and 16S rRNA gene sequencing. Fifteen strains exhibited antimicrobial activity against Gram-positive bacteria. Strain CR1-09T, proposed as Microbispora cathalanthi sp. nov. was active against Staphylococcus aureus ATCC 6538 and Kocuria rhizophila ATCC 9341. Streptomyces sp. 3MP-10 and 3MP-14, novel species were active against S. aureus ATCC 6538, K. rhizophila ATCC 9341, and Bacillus subtilis ATCC 6633. Strains CR1-09T and PA1-07 showed cytotoxicity against HepG2 …

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Physicochemical, Permeability, Antioxidant And Anti-Inflammatory Properties Of Oxyresveratrol Ester Prodrugs, Wuttinont Thaweesest Jan 2019

Physicochemical, Permeability, Antioxidant And Anti-Inflammatory Properties Of Oxyresveratrol Ester Prodrugs, Wuttinont Thaweesest

Chulalongkorn University Theses and Dissertations (Chula ETD)

Oxyresveratrol (OXY) is known to exert antioxidant and anti-inflammatory properties but has low oral bioavailability in rats and low permeability in the Caco-2 cell monolayer model. Three ester prodrugs with different acyl chain lengths (oxyresveratrol tetraacetate (OXY-TAc), oxyresveratrol tetrapropionate (OXY-TPr), and oxyresveratrol tetrabutyrate (OXY-TBu) were synthesized to improve the permeability of OXY. Transport of three OXY ester prodrugs across Caco-2 monolayers was higher than that of OXY, and OXY-TAc exhibited the highest permeation. The degradation of OXY-TAc in HCl buffer (pH 1.2), phosphate buffer (pH 6.8 and 7.4), simulated gastric and intestinal fluids, as well as human plasma at 37 …

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In Vitro Hepatic Metabolism Of Curcumin Diethyl Disuccinate In Different Animal Species, Ponsiree Jithavech Jan 2019

In Vitro Hepatic Metabolism Of Curcumin Diethyl Disuccinate In Different Animal Species, Ponsiree Jithavech

Chulalongkorn University Theses and Dissertations (Chula ETD)

Curcumin diethyl disuccinate (CDD) is an ester prodrug of the active compound, curcumin. CDD has been improved chemical stability and intestinal permeability more than curcumin. CDD demonstrated biological activity higher than curcumin. The aim of this study is to investigate a suitability of using liver S9 fraction (LS9) in drug metabolism profiling as function of animal sources of enzyme regarding possible translation of in vitro outcome to clinical study. The metabolites, in vitro kinetics and esterase enzyme involved in CDD hydrolysis by LS9 of human, monkey, dog and rat ware determined. The high-performance liquid chromatography conjugated with high-resolution mass spectrometry-based …

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Production Optimization Of Recombinant Human Vascular Endothelial Growth Factor In Tobacco (Nicotiana Benthamiana), Christine Joy I. Bulaon Jan 2019

Production Optimization Of Recombinant Human Vascular Endothelial Growth Factor In Tobacco (Nicotiana Benthamiana), Christine Joy I. Bulaon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Molecular pharming represents the innovative development of plants as alternative production platforms for recombinant proteins. Human vascular endothelial growth factor (VEGF) is one of the growth factors that has displayed eminent roles in wound healing. This study aimed to develop optimized expression conditions and rapid production of recombinant VEGF in Nicotiana benthamiana. Herein, four plant expression constructs, particularly pBYR2e-SP-VEGF-His-SEKDEL (S-V-H-K), pBYR2e-SP-VEGF-His (S-V-H), pBYR2e-SP-His-VEGF-SEKDEL (S-H-V-K) and pBYR2e-SP-His-VEGF (S-H-V), were utilized and inserted into geminiviral vector pBYR2e-K2Md for transient expression in N. benthamiana. The expression level of all the four constructs was examined and the optimal harvest time for high protein expression …

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Development Of Mucoadhesive Biloniosome Nanoparticles For Enhanced Gastrointestinal Absorption Of Cordycepin, Warut Kengkittipat Jan 2019

Development Of Mucoadhesive Biloniosome Nanoparticles For Enhanced Gastrointestinal Absorption Of Cordycepin, Warut Kengkittipat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cordycepin, an adenosine analogue, has displayed numerous pharmacological and therapeutic implications including enhanced apoptosis and inhibition of proliferation of cancer cells, antioxidant property and improving sexual activity. However, oral administration of cordycepin has limited practical use due to its poor bioavailability in the intestine. Here, we developed a novel hybrid nanoparticle system for effective delivery of cordycepin in the gastrointestinal tract by using the bile salt-based liposome/niosome hybrid system (designated as biloniosome). In addition, mucoadhesive chitosan biopolymer was coated on the biloniosome nanoparticles to improve the mucoadhesive properties thereby enhancing the drug efficiency. Cordycepin-encapsulated biloniosome nanoparticles were synthesized by solvent …

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Dna Barcoding Coupled With High Resolution Melting (Bar-Hrm) Analysis For Differentiation White Kwao Khruea (Pueraria Candollei), Black Kwao Khruea (Mucuna Macrocarpa), And Red Kwao Khruea (Butea Superba), Nonthapon Vejpanich Jan 2019

Dna Barcoding Coupled With High Resolution Melting (Bar-Hrm) Analysis For Differentiation White Kwao Khruea (Pueraria Candollei), Black Kwao Khruea (Mucuna Macrocarpa), And Red Kwao Khruea (Butea Superba), Nonthapon Vejpanich

Chulalongkorn University Theses and Dissertations (Chula ETD)

Kwao Khruea is the herbal product that known as a rejuvenation herb. In Thai traditional medicine, three species of Kwao Khruea including white Kwao Khruea (Pueraria candollei Wall. ex Benth.), black Kwao Khruea (Mucuna macrocarpa Wall.) and red Kwao Khruea (Butea superba Roxb. ex Willd.) have been used. Each Kwao Khruea accumulates different chemical constituents. White Kwao Khruea contains estrogenic biological activities while black and red Kwao Khruea accumulate testosterone-like activities. Black Kwao Khruea was reported to be potent than red Kwao Khruea. Because of similar common names with the difference in chemical compositions of Kwao Khruea herbs, it is …

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Free Radical Scavenging Compounds From Dendrobium Lindleyi, Pichayatri Khoonrit Jan 2019

Free Radical Scavenging Compounds From Dendrobium Lindleyi, Pichayatri Khoonrit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Free radicals lead to oxidative stress which plays an important role in the development of many diseases. Dendrobium lindleyi belongs to Dendrobium, a genus the secondary metabolites of which were reported as free radical scavenging compounds. However, the bioactivity of D. lindleyi has never been revealed. In this study, the EtOAc extract of D. lindleyi was fractionated to give five pure compounds identified by spectroscopic techniques as a new constituent named 4,5-dihydroxy-3,3′,4′-trimethoxybibenzyl along with four known compounds including chrysotoxine, gigantol, cypripedin, and moscatilin. DPPH radical, superoxide radical, and ORAC assay were performed to evaluate their radical scavenging activities. Chrysotoxine, gigantol, …

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Synthesis And Characterization Of Polyethylene Glycol-Doxorubicin Conjugates Containing Β-Thiopropanamide Linkers For Enhanced Selective Cytotoxicity Against Breast Cancer Cells, Wiwat Supasena Jan 2019

Synthesis And Characterization Of Polyethylene Glycol-Doxorubicin Conjugates Containing Β-Thiopropanamide Linkers For Enhanced Selective Cytotoxicity Against Breast Cancer Cells, Wiwat Supasena

Chulalongkorn University Theses and Dissertations (Chula ETD)

Doxorubicin (DOX) is a chemotherapeutic agent that suffers from its severe adverse effects due to non-selective cytotoxicity. This study aims to design novel beta-thiopropanamide linkers (-S-CH2-C(R)H-CONH-) for DOX conjugation to promote cancer cell selectivity and cytotoxicity. Two series of DOX conjugates with various beta-thiopropanamide linkers were synthesized and evaluated for their cytotoxic selectivity. The first series contains 7 DOX analogues with different beta-thiopropanamide linkers. The DOX analogues were synthesized using ethyl thioglycolate and acrylic acid analogues to establish a series of beta-thiopropanamide linkages with DOX, and the potential linkers were intended to be incorporated in polymer-DOX conjugates. The other series …

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Development Of Cyclodextrin-Based Pickering Nanoemulsion Containing Amphotericin B For Treatment Of Fungal Keratitis, Phyo Darli Maw Jan 2019

Development Of Cyclodextrin-Based Pickering Nanoemulsion Containing Amphotericin B For Treatment Of Fungal Keratitis, Phyo Darli Maw

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to develop pickering nanoemulsions (NEs) containing amphotericin B (AmB) for fungal keratitis therapy. The phase-solubility profiles of AmB in various aqueous cyclodextrin (CD) solutions were determined. The AmB solubilization in oils and surfactants was also examined. Two CDs i.e., gCD and 2-hydroxypropyl-g-CD (HPgCD), medium-chain triglycerides (MCT oil) and phosphatidylcholine (lecithin) were chosen because of their high solubility increment of AmB. Solution-state by 1H-NMR studies demonstrated the formation of MCT oil/αCD, AmB/gCD and AmB/HPgCD complexes. Some interactions between AmB and CD have been observed by solid-state characterizations (FT-IR, PXRD and DSC). The interfacial tension at …

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Expression Of Human Dentin Matrix Protein 1 In Escherichia Coli And Nicotiana Benthamiana And Their Effect On Proliferation And Osteogenic Differentiation Of Human Periodontal Ligament Stem Cells, Aktsar Roskiana Ahmad Jan 2019

Expression Of Human Dentin Matrix Protein 1 In Escherichia Coli And Nicotiana Benthamiana And Their Effect On Proliferation And Osteogenic Differentiation Of Human Periodontal Ligament Stem Cells, Aktsar Roskiana Ahmad

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to express recombinant protein human Dentin Matrix Protein 1 (hDMP1) in Escherichia coli and Nicotiana benthamiana and investigated the osteogenic differentiation effects in human Periodontal Ligament Stem Cells (hPDLSCs). The gene encoding hDMP1 was ligated into the pET22b and pBY2R2e expression vectors. Recombinant hDMP1 protein has been productively expressed in E. coli and N. benthamiana. E. coli-produced hDMP1 resulted in 60 kDa and 100, and the optimal condition to produce highly expression was demonstrated at 0.5 mM IPTG and incubation at 37oC for 6 hr. N. benthamiana-produced hDMP1 protein showed in a 100 kDa that indicated the …

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