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Modeling The Condensed-Phase Behavior Of Π-Conjugated Polymers, Walker Mask

Theses and Dissertations--Chemistry

It is well established that the morphology and physical properties of an organic semiconducting (OSC) material regulate its electronic properties. However, structure-function relationships remain difficult to describe in polymer-based OSC, which are of particular interest due to their robust mechanical properties. If relationships among the molecular and bulk levels of structure can be found, they can aid in the design of improved materials. To explore and detail important structure-function relationships in polymer-based OSC, this work employs molecular dynamics (MD) simulations to study various π-conjugated polymers in different environments. Two independent investigations are discussed in this work. One investigation examines how …

Detection Of Beta-Lactamase Variants In Municipal Wastewater And Fresh Water, Sunil Pandey

Masters Theses

The occurrence and spread of antibiotic-resistant genes (ARGs) are pressing public health problems worldwide. A key factor contributing to the spread of ARGs is lateral gene transfer. Wastewater treatment plants (WWTPs) are measured hot spots of microbial diversity and resistance because they receive polluted wastewater from diverse sources and contain a variety of different environments with dense bacterial loads. Due to the overuse of antibiotics the genetic capacities of microbes have profited. This helps every source of resistance gene and every means of horizontal gene transmission to develop the multiple mechanism of resistance to each antibiotic used clinically, agriculturally, or …

Support From Above The Glass Ceiling: Narratives Of Women As University Student Leaders, Rebecca Schwartz

Masters Theses

Using qualitative methodology. the researcher examined motivations, social support networks, and challenges college women face in student leadership positions. Four semi-structured interviews were conducted at a midsized university in the Midwest with college women in various positions of organizational leadership. The researcher identified motivational factors for women to apply for leadership positions as well as described the social support network that exists for college women in positions of leadership. Challenges college women face in achieving higher leadership positions were also identified. Lastly, recommendations were made for student affairs professionals and women in leadership based on the research findings.

College Students Experiences Participating In International Alternative Break Trips, Dayton L. Ehrlich

Masters Theses

This study sought to explore undergraduate students’ experiences participating in international alternative break trips. A qualitative approach was used to understand the motivating factors as well as the impact the trip had on them. Participants included three female undergraduate students who had participated in an international alternative break trip in Mexico were interviewed one-on-one. The results demonstrated there were multiple different motivating factors and impacts the students experienced. The motivating factors consisted of student involvement, they were all personally invited on the trip, practicing a new language, opportunity to leave the country, and service was not a motivator. The impacts …

Construct Validity Of The Teate Depression Inventory (Tdi) With A Middle Eastern/Arab American Sample, Dalia Bunni

Masters Theses

The purpose of the study was to examine the construct validity of a newer test used to measure depressive symptoms, the Teate Depression Inventory (TDI). The primary focus of the study was on Middle Eastern/Arab Americans (ME/AA). Previous research has demonstrated that ethnic minority groups may experience and present internalizing disorders, such as depression and anxiety differently than the majority ethnic group, White/Caucasian (W/C) individuals. Further, research suggests that there is a disparity in mental health care among ethnic minority groups, starting with detecting and diagnosing mental health disorders. Inaccurate detection and diagnoses informs inaccurate treatment, further creating a disparity. …

A Pipeline For Exogenous Gene Expression And Biomass Analysis In Plants, Bijay Bisunke

Masters Theses

Biological pretreatment of biomass is typically required for the production of liquid biofuels, which are viable alternatives to traditional fossil fuels. While traditional thermomechanical pretreatments are effective at helping to deconstruct lignocellulose, they are often expensive and tend to generate environmentally dangerous waste materials. An alternative to traditional pretreatment strategies is biological pretreatment, which has focused on lignocellulose-degrading organisms, such as white-rot fungi. While direct pretreatment of biomass with white-rot fungi has shown great promise, directly expressing functional white-rot fungi genes in plants (i.e. in planta expression) has the potential to be even more efficient because the biomass itself …

Study Of Genetic Regulators That Control Development And Secondary Metabolism In The Genus Aspergillus, Timothy Satterlee

Graduate Research Theses & Dissertations

In this study, I investigated the role of two different regulatory genes in two different species of pathogenic fungi from the genus Aspergillus. The first study involves a transcriptome analysis of the epigenetic regulator rmtA in the plant pathogen Aspergillus flavus. A. flavus colonizes numerous oil seed crops such as corn, peanuts, treenuts and cotton worldwide, contaminating them with aflatoxins and other harmful potent toxins. Previously our lab characterized the gene rmtA, which encodes an arginine methyltransferase in A. flavus, and demonstrated its role as regulator of the expression of the aflatoxin gene cluster and concomitant synthesis of toxin. Furthermore, …

Functions Of Dfrp And Other Drg Pathway Genes In The Moss Physcomitrella Patens, Robert James Srygler

Graduate Research Theses & Dissertations

GTP binding proteins (G proteins) are molecular switches that regulate many aspects of physiology in all cells. DRG1 and DRG2 (Developmentally Regulated GTP-binding proteins) have been found in all eukaryotes examined. Amino acid identity across eukaryotes is about 65% within an orthologous group and 55% between paralogs of a single species. Although DRGs are likely involved in some aspect of translation regulation, details of their precise cellular functions are not known.

In addition to DRGs there are other genes/proteins that comprise the “DRG pathway.” DFRPs (DRG Family Regulatory Proteins) have been shown to physically and genetically interact with DRGs. Previous …

Development Of Synthetic Methodologies Of Nucleophile-Electrophile Chemistry, Michael R. Stentzel

Graduate Research Theses & Dissertations

The work outlined in this dissertation describes the development of synthetic methodologies utilizing a wide electrophilicity range of substrates towards the synthesis of new compounds. Substitution, addition, and superelectrophilic chemistry are powerful tools to afford potentially important compounds. These concepts are summarized in chapter 1.

Chapter 2 describes the capture of superacid catalyzed fluorenyl cations with nitriles (Ritter reaction) and alcohols. Optimization of reaction conditions and extensive scope studies were performed.

In chapter 3, the Michael addition of organometallic reagents with N-heterocyclic substituted olefins is explored. The nucleophilic carbanion intermediate species is shown to have reactivity towards strong electrophiles. With …

The Design, Synthesis, And Evaluation Of Inhibitors Of The Ispf Enzyme In The Mep Pathway, Christopher G. Stewart

Graduate Research Theses & Dissertations

Isoprenoids are a class of over 50,000 compounds that serve as key precursors for many compounds in plants and animals. These compounds play critical roles in many biological functions such as cellular growth, respiration, and go on to form critical compounds such as steroids and vitamins. Despite their overall necessity to life their biosynthesis only follows two synthetic pathways. These pathways are the Mevalonic Acid (MVA) Pathway and the 2-C-Methyl-D-Erythritol-4-Phosphate (MEP) Pathway also known as the non-mevalonate pathway. The MVA pathway is found primarily in humans and some higher plants while the MEP pathway is found in bacteria and some …

Why Do Symbiodinium Migrate? Evolutionary Conflict In A Host-Symbiont Community, Jacey Lynn Strohecker

Graduate Research Theses & Dissertations

Climate change is an increasing problem for species all around the globe, including coral reef communities, which are among the most diverse and productive in the world. The mutualistic symbiosis between corals and photosynthetic dinoflagellates (symbionts) is central to these ecosystems. The rising temperatures are causing corals to lose their symbionts in a process known as bleaching, thus endangering the entire community. In this context, it is imperative to discover mechanisms that might mitigate the effects of stress on corals. Previous work has established that stress increases the formation of reactive oxygen species (ROS), that is, partially reduced forms of …

Investigation Of An Inquiry-Based Learning Intervention On Undergraduate Saudi Women’S Critical Thinking Skills In An English As A Foreign Language Reading And Writing Class During The Preparatory Year, Rehabe Tikruni

Graduate Research Theses & Dissertations

The purpose of this study was to investigate the implementation of an inquiry-based learning (IBL) intervention and its influence on the critical thinking (CT) skills of Saudi women undergraduates enrolled in an English as a Foreign Language (EFL) reading and

writing course during the preparatory year. This study utilized a qualitative case study design from one public university in Saudi Arabia during the second semester of the preparatory year English program in Fall 2018. Thirty-five women participated in the IBL intervention

and the pre- and post-reflective essays. To delve deeper into perceptions about IBL and its potential influence on critical …

Discovery Of Selective Probes Targeting Rna Polymerase I, Xiao Tan

Theses and Dissertations--Chemistry

RNR Polymerase I (RNA Pol I) is a “factory” that orchestrate the transcription of ribosomal rRNA for constructing ribosomes as a primary workshop for protein translation to sustain cell growth. Misregulation of RNA Pol I can cause uncontrolled cell proliferation, which leads to the development of cancer. Yeast (Saccharomyces cerevisiae) is a valuable model system to study RNA Pol I. Recently, the X-ray crystal structure of the yeast homologue of RNA Pol I was elucidated, offering the structural basis to selectively target this transcriptional machinery. The approach to selective RNA Pol I targeting was to disrupt the interaction …

Investigation Of Thiol-Containing Biomarkers And Their Role In The Exposome, Tracy Gastineau-Stevens

Theses and Dissertations--Chemistry

Exposomics is an emerging area of study that looks at how the environment around a person or persons affects their overall health. Biomarkers have emerged as useful tools to better understand how the exposome affects a person. In this work, we investigate two potential endogenous biomarkers, homocysteine and glutathione that have been previously implicated in a number of diseases that have been linked to environmental causes. We also investigated an environmental exposure, the fungicide Ziram, which epidemiologically has been linked to diseases. In our investigation, we utilized capillary electrophoresis and capillary electrophoresis-mass spectrometry to develop a method for homocysteine and …

Multi-Scale Computational Studies Of Calcium (Ca2+) Signaling, Bin Sun

Theses and Dissertations--Chemistry

Ca²⁺ is an important messenger that affects almost all cellular processes. Ca²⁺ signaling involves events that happen at various time-scales such as Ca²⁺ diffusion, trans-membrane Ca²⁺ transport and Ca²⁺-mediated protein-protein interactions. In this work, we utilized multi-scale computational methods to quantitatively characterize Ca²⁺ diffusion efficiency, Ca²⁺ binding thermodynamics and molecular bases of Ca²⁺-dependent protein-protein interaction. Specifically, we studied 1) the electrokinetic transport of Ca²⁺ in confined sub-µm geometry with complicated surfacial properties. We characterized the effective diffusion constant of Ca²⁺ in a cell-like environment, which helps to understand …

The Experiential-Learning Track: Career Exploration, Student Interest, & Applied Classroom-Learning In Small Rural Junior-Senior High Schools, Michael Turnlund

Northwest Journal of Teacher Education

Budgetary constraints can limit academic programs and elective offerings in small rural high schools. It is difficult to staff schools with teachers who hold the required credentials to offer elective courses that match student interests and future career or post-secondary education goals. This article details a strategy used by one small school in Idaho to address the problem. The Experiential-Learning Track Program allows for “hands-on” opportunities tied to learning objectives, and targets career and post-secondary education goals of individual students. Outcomes of the project included an increase in student population numbers and more connections with the local community.

Psyc 462/862: Motivation And Emotion, Carolyn R. Brown-Kramer

UNL Faculty Course Portfolios

In this course portfolio, I examine the course goals, learning objectives, teaching methods and materials, assessments, and outcomes for an upper-level psychology elective course, PSYC 462/862, Motivation and Emotion. The course introduces students to the major theories of motivation and emotion, develops their ability to critically examine empirical psychological research, encourages the application of theoretical and empirical work to real-world contexts, and strengthens students’ communication skills. For each of the four course goals, I describe how and why I conduct the course as I do, as well as how my pedagogical choices influence student learning and engagement. Data analyses and …

Psyc/Wmns 421/821: Psychology Of Gender, Kathryn Holland

UNL Faculty Course Portfolios

This benchmark portfolio describes the process of developing and assessing student learning in Psychology of Gender (PSYC/WMNS 421/821)—a course I taught in Spring 2019 while participating in the Peer Review of Teaching Project at the University of Nebraska Lincoln. This course is housed in the Psychology Department and cross-listed in Women’s and Gender Studies. The students in this course are mostly advanced undergraduate psychology majors. The course is capped at 40 and is primarily discussion-based. In my portfolio, I outline the course structure and context, my course objectives, and the students enrolled. I also document the teaching materials, methods, and …

Course Portfolio For Assessing Student Learning Surrounding Biological Examples In Bsen244: Thermodynamics Of Biological Systems, Forrest M. Kievit

UNL Faculty Course Portfolios

Thermodynamics is a course required in many engineering disciplines as it covers concepts utilized in many upper level engineering courses. In the Biological Systems Engineering Department at the University of Nebraska – Lincoln, this course, in a way, acts as a gateway to the major since this will be one of the first classes where the students must apply and hone their problem-solving skills. This course was developed to allow students in the department to have access to a thermodynamics course that relates to their major area of interest – biological systems. However, many concepts in thermodynamics do not have …

Bioc 321: Elements Of Biochemistry – A Peer Review Of Teaching Project Benchmark Portfolio, Jing Zhang

UNL Faculty Course Portfolios

The benchmark portfolio of Biochemistry 321, Elements of Biochemistry, aimed to provide a broad overview for this large-enrollment class for non-biochemistry majors. This course was delivered in a blended format, and the learning objectives were aligned with the American Society of Biochemistry and Molecular Biology (ASBMB) foundational concepts and skills. Classroom response system iClicker was employed and Think-Pair-Share method was utilized to facilitate the in-class discussion and group-learning. Additional activities include the 3D molecular models for visualizing and hands-on learning of the structure-function relationship concept, and practicing problem-solving through the end-of-chapter essay questions. Data analysis suggested that the iClicker response …

Peer Review Of Teaching Benchmark Portfolio For Soil 477/877: Great Plains Field Pedology, Judith Turk

UNL Faculty Course Portfolios

Pedology is a field that focuses on soil morphology, genesis, classification, and mapping. Students in pedology must learn to be keen observers, who are able to accurately evaluate the characteristics of the soil. This portfolio focuses on efforts to improve students’ skills at describing soil color, texture, and morphologic features.

Slpa 985: A Peer Review Of Teaching Benchmark Portfolio, Judy Harvey

UNL Faculty Course Portfolios

My target course for the Peer Review of Teaching (PRT) Project was SLPA 985 Traumatic Brain Injury (TBI). It is offered each spring in the Department of Special Education and Communication Disorders at the University of Nebraska – Lincoln. This course is intended for graduate level speech language pathology (SLP) students. I have over twenty years of experience as a practicing speech language pathologist in a rehabilitation hospital. Now, as an assistant Professor of Practice, my role is to integrate academic learning with practical experience. I am confident in my skills as a practitioner and motivated to share my knowledge, …

Fdst 396 – Preventive Controls For Animal Food, Andreia Bianchini

UNL Faculty Course Portfolios

This portfolio is a benchmark for the course Preventive Controls for Animal Food, which was offered for the first time in Spring 2019. The objectives of this course were (1) to provide students with the ability to distinguish between Current Good Manufacturing Practices (cGMPs), other prerequisite programs, preventive controls, and where they fit into the regulatory framework of the Food Safety Modernization Act – Preventive Controls for Animal Food; (2) to help students understand the hazard analysis process and resources to help conduct such analysis; and (3) to teach students the needed concepts to build a food safety plan. This …

Minimalistic Supramolecular Proteoglycan Mimics By Co-Assembly Of Aromatic Peptide And Carbohydrate Amphiphiles, Alexandra Brito, Yousef M. Abul-Haija, Diana Soares De Costa, Ramon Novoa-Carballal, Rui L. Reis, Rein V. Ulijn, Ricardo A. Pires, Iva Pashkuleva

Advanced Science Research Center

We report the co-assembly of aromatic carbohydrate and dipeptide amphiphiles under physiological conditions as a strategy to generate minimalistic proteoglycan mimics. The resulting nanofibers present a structural, fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) core and a functional carbohydrate (Fmoc-glucosamine-6-sulfate or -phosphate) shell. The size, degree of bundling and mechanical properties of the assembled structures depend on the chemical nature of the carbohydrate amphiphile used. In cell culture medium, these nanofibers can further organize into supramolecular hydrogels. We demonstrate that, similar to proteoglycans, the assembled gels prolong the stability of growth factors and preserve the viability of cultured cells. Our results demonstrate that this approach …

Triumph Of The Nomothetic: Personality Assessment And Forensic Psychology, Marvin W. Acklin

Archives of Assessment Psychology

This study locates personality assessment within the discipline of forensic psychology, examining the forensic relevance of major paradigms of personality assessment -- psychodynamic, interpersonal, personological, multivariate, empirical, and neuroscience -- with a focus on the clinical and forensic assessment of psychopathy. A credible personality paradigm must produce testable hypotheses, have associated assessment measures, and have a supporting empirical research literature (Wiggins, 1973). In the first survey, a systematic literature of the PsycINFO database revealed that all of the major personality assessment paradigms satisfy criteria for scientific credibility with the exception of the personological. In the second survey, the status of …

Medicinal Properties Of The Araliaceae, With Emphasis On Chemicals Affecting Nerve Cells, Rana Alharbi

Masters Theses

In recent times, medicinal plants have become the focus of scientists and research. However, many have used traditional medicine to take advantage of plant extracts to treat many diseases, especially neurological diseases. Various central nervous system receptors have been shown to associate with plant extracts influencing the pharmacology and in this manner conceivably assuming a role in human sickness and treatment. For instance, extracts from Cussonia paniculata Eckl. & Zeyh targeted several early nervous and mental disease, and Kalopanax pictus has been traditionally used for the treatment of rheumatoidal arthritis, nerve pain and diabetes mellitus.

In the present study, we …

Context Dependency Of Invasion Impacts On The Controllers Of Invasibility In Microstegium Vimineum, Scott Vincent Janis

Masters Theses

Although plant invasions are of major conservation concern, understanding of the natural controls on invasion and their impacts is largely limited to static observational studies or artificially manipulated systems. Linking patterns of invasion with subsequent impacts in natural systems is necessary to fully assess invasion causes and consequences. Therefore, I employed a long-term approach to sequentially assess the controls on invasion and their subsequent impacts in a self-assembled system for the highly invasive annual grass Microstegium vimineum. I modeled likely factors contributing to the probability of invasion and local invasion success of a natural invasion of M. vimineum in a …

Assessment And Recovery Of Stream Restoration Efforts On Fish Communities, Jessica Marie Rohr

Masters Theses

Aquatic systems are subjected to disturbances of various types, including natural and anthropogenic, or can deteriorate due to accumulating unfavorable conditions, including receding banks, decreasing riparian vegetation, and disrupted flow patterns. An analysis was done on a variety of streams in central Illinois that experienced one or multiple anthropogenic disturbances without remediation to determine the natural recovery dynamics of each system. A separate analysis was performed on a multi-site restored stream with a complex restoration project with a variety of restoration methods. This data were collected over at periods of time spanning from 5-15 years, including time prior to disturbance/restoration …

Impacts Of Plant-Microbe Interactions On Seedling Performance In A Riparian Forest Invaded By Lonicera Maackii, Taylor E. K. Strehl

Masters Theses

Soil microbes have profound impacts on plant growth and survival and can either promote or inhibit plant dominance. Exotic plants are often strongly invasive because they have escaped their natural enemies, potentially including antagonistic soil microbes. I examined how the invasive shrub Lonicera maackii and a common native tree, Acer negundo, responded to soil microbial communities to determine the role of soil microbes in regulating invasion success. This was done by growing both species with microbes from invaded (L. maackii) and uninvaded (A. negundo) soils collected from three locations within a riparian forest. Seedlings were …

Identifying Priority Conservation Areas And Strategies For Myotis Sodalis (Chiroptera: Vespertilionidae) Via Habitat And Connectivity Modeling, Ashleigh B. Cable

Masters Theses

Myotis sodalis, the Indiana bat, is a federally endangered bat species in the United States of America (USA). Conservation efforts are typically focused at identified maternity sites at local scales, however, the species is a regional migrant that interacts with its environment at multiple spatial scales. We are limited in our knowledge of landscape-level requirements of this species, especially in large areas such as Illinois, USA, where a wide range of environmental and landscape conditions exist. Many previous M. sodalis habitat studies have limited their focus to smaller spatial scales. Due to limitations in funding, personnel, and time, it …