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The Treatment Of Contaminated Water Using Electrocoagulation, Adebayo Edward Adejinle

Graduate Research Theses & Dissertations

Wastewater treatment is a process that is used to remove significant amount of contaminant concentration from wastewater, which prevents harm to the environment. Chemical coagulation (CC) is one of the most influential processes used to remove contaminants, and it is the addition of chemicals called coagulants, which causes particles to stick together forming bigger flocs. However, as effective as CC is, it requires bench scale testing for determining the appropriate dosing rate for specific raw water properties, which makes the process time consuming. To eliminate these issues, the introduction of the Electrocoagulation (EC) process is considered. The EC process is …

The Link Between Antibiotic Resistance Used For Recombinant Dna Selection And Cellular Phenotype In Rat Liver Epithelial Cells, Doaa Alshammari

Graduate Research Theses & Dissertations

Recombinant DNA plasmids are often used to mediate forced gene overexpression or suppression in mammalian cells, to address questions regarding the role of specific proteins in cell behavior and function. To select for cells harboring such recombinant plasmids, lethal antibiotics such as Puromycin, Geneticin (Neomycin) and Zeocin are frequently used, as plasmids contain selective antibiotic resistance genes that biochemically disarm them. Antibiotic resistance, induced by plasmid-borne genes and chronic exposure to lethal antibiotics can likewise potentially change the phenotype of cells, confounding the conclusions drawn from experiments on gene overexpression or suppression. Yet, little is known about the effects of …

Lewis Base Promoted Annulations Of Allenoates With 1,4-Naphthoquinones, Nicole Marie Shoup

Electronic Theses and Dissertations

Lewis base promoted cyclization of allenoates with electron deficient olefins open up the possibility to generate more complex organic structures. By generating more complex molecules, we are able to produce new compounds for biomedical and material applications. Both phosphine and amine Lewis bases offer interesting annulations with allenoates resulting in Lu or Morita–Baylis–Hillman type reactions. Once the Lewis base engages with the allenoate there can be several modes of nucleophilic reactivity that allows for the allenic ester to act as a two or three carbon synthon. The allenoate can then react with varying electron deficient olefins generating more complex molecules …

Utilization Of Superelectrophiles In Organic Synthesis, Hien Quoc Vuong

Graduate Research Theses & Dissertations

This dissertation describes the use of triflic acid as a catalyst in organic synthesis. The first chapter is an introduction of reaction classes, reactive intermediate, and superacids.

Chapter 2 discusses a series of compounds have been prepared by the Fischer indole synthesis and Friedel–Crafts reactions, using a single acid catalyst in a one-pot conversion. The reactions have worked best with pyridyl-substituted hydrazones/indoles. Chlorination and bromination products are formed in good yields and high regioselectivities. Acylation products have also been prepared by the two-stage reaction. In the case of alkylation, the expected alkyl-substituted indoles are obtained however they are accompanied by …

Wellness In Asexual-Identified Individuals: The Impact Of Social Support And Microaggressions, Elisa Marie Woodruff

Graduate Research Theses & Dissertations

One hundred sixty asexual identified individuals living in the United States were recruited from asexual online communities. The participants completed a comprehensive survey consisting of Zimet et al.’s 1988 Multidimensional Scale of Social Support, Foster’s 2017 Asexual Microaggression Scale, and the Five Factor Wellness Inventory Adult Version Revised (FFWEL-A2) developed by Myers and Sweeney in 2014, as well as 15-item demographic items. Regression models were used to assess relationships among the factors.

Social support was found to have a significant positive impact on participant wellness, supporting previous social science research. Microaggressions were not found to have a negative impact on …

Coupling Reactions Of Triaryl Carbocations Via Cationic Charge Delocalization, Hongfang Xue

Graduate Research Theses & Dissertations

This thesis describes a coupling reaction involving cationic charge delocalization. This work began as a study of the Piancatelli rearrangement, a reaction involving furan-based triaryl methyl cations. A new synthetic reaction has been discovered using these furan-based triaryl methyl cations as electrophilic intermediates. Zinc chloride has been found to be an effective Lewis acid catalyst for the ionization of the triaryl methanols to the carbocations. It has been found that furan-based triaryl methyl cations react with nucleophiles such as enol silyl ethers and silyl ketene acetals. Similar to the Piancatelli reaction, nucleophilic reactions are found to occur at the 5-position …

Holistic Analysis Of The Social, Emotional, And Academic Development Of At-Risk Students In A Self-Regulated Learning Instructional Environment, Jennifer K. Macek

Graduate Research Theses & Dissertations

The testing an accountability system that plagues the current educational atmosphere has done little to improve outcomes for the general population, rather, it has increased harm to our nation’s most vulnerable populations. As districts grapple with accountability mandates and pressures to improve test scores the number of students excluded from traditional schools and sent to alternative programs continues to grow. Research shows that the majority of programs for at-risk youth are little more than graduation factories that do not adequately prepare students for active participation in democratic society.

This study used a mixed methods design to explore an alternative program …

Water Quality And Small Unmanned Aerial System Photogrammetry Of Boeng Cheung Ek Wastewater Treatment Wetland, Phnom Penh, Cambodia, Samuel Vesal Mallow

Graduate Research Theses & Dissertations

Boeng Cheung Ek wetland is a large, peri-urban, free water surface, natural wastewater treatment wetland that services approximately 90% of combined storm water and untreated sewage from the city of Phnom Penh, Cambodia, population over 1.5 million. Due to monsoon rainfall and connectivity to the Mekong River, the wetted area cycles between 13 km2 and 20 km2 annually. The objective of this study is to characterize the spatiotemporal variability of contaminants in wetland surface water and groundwater. Dissolved ions including ammonium, nitrate-N (as NO3), phosphate, and chloride, along with E. coli, trace metals, δH, δ18O, and physicochemical parameters were determined …

An Experimental Examination Of Intolerance Of Uncertainty In Obsessive-Compulsive Disorder, Hannah Faleer Martyn

Graduate Research Theses & Dissertations

Anxiety is considered a fear response to threat, driven by the absence of certainty and elevated perceptions of the cost of harm. It has been suggested that exaggerated responding to uncertainty underlies all anxiety disorders. Although no longer classified under the anxiety disorders section of the Diagnostic and Statistical Manual (DSM), anxiety still is considered a primary component of obsessive-compulsive disorder (OCD). OCD is a chronic, debilitating condition that affects multiple domains of functioning. The current gold standard of treatment for OCD is exposure and response prevention (ERP), a form of cognitive-behavioral psychotherapy. However, ERP is not effective for all …

Hydrogeochemical Characterization Of Yalahau Region, Mexico, Jessica Leann Mckay

Graduate Research Theses & Dissertations

The Yalahau Region, located in the northeastern portion of the Yucatán Peninsula, hosts a series of elongated depressions trending north/south in the direction of Isla Holbox, identified as the Holbox Fracture Zone. Previous studies have explored the geomorphology and various hydrologic characteristics of the Yucatán Peninsula; however, there is a paucity of data concerning the interior region where the fractures are located. Strontium isotope and major ion geochemistry data of the surface water and groundwater of this region serves as a hydrogeochemical fingerprint, aiding to constrain the hydrological boundaries, determining flow paths, and characterizing hydrogeochemical processes that impact the composition …

Contesting Consent: Consent, Sexual Scripts, And Gendered Power, Julia Kristine Metz

Graduate Research Theses & Dissertations

Within the past decade, the United States has seen an upsurge of affirmative consent policies being introduced or passed within the legislation of state governments and universities (Laker and Boas 2015). However, in a moment when the President of the US has been recorded saying “Grab ‘em by the pussy,” two Supreme Court justices have been accused of sexual misconduct, and a Secretary of Education has rolled back Title IX policies that had made progress in curbing sexual violence in schools, the meaning of sexual consent is now being contested more than ever. However, there have only been a handful …

Hif-2 Regulation Of Amino Acid Transporter Expression In Hepatocellular Carcinoma Cells, Jason A. Misurelli

Graduate Research Theses & Dissertations

Hypoxia represents a major driver of the cancer phenotype in solid tumors, especially those of hepatic origin. Low oxygen availability triggers a complex cellular response which alters metabolism and promotes cellular invasion. Growing evidence suggests the amino acid glutamine, an important molecular currency for both energy producing reactions and biosynthetic pathways, plays an enhanced role in cancer cells under hypoxia. Hypoxia-inducible factors (HIFs) are transcription factors crucial for regulating the cellular response to low oxygen. Family member HIF-1 has been well studied, however, recent evidence suggests HIF-2 may have a regulatory role in amino acid transport and metabolism under hypoxia. …

Unsc Legitimacy As A Tool For Misdirection, Medha Monjaury

Graduate Research Theses & Dissertations

In the months leading up to the 2003 invasion of Iraq, the Bush administration made clear to the American people that the United States was prepared to go to war if Iraq failed to comply with Resolution 1441 and disarm. However, during the process of drafting and passing Resolution 1441, the U.S. expended considerable time and energy maintaining to the United Nations Security Council that it would not use the resolution as a pretext to strike Iraq. Moreover, it appears that the Security Council was convinced of the U.S.’ stated intentions when it passed Resolution 1441 unanimously in November 2002, …

Ground Beetle Community Structure And Function In Restored Tallgrass Prairie, Melissa Nelson

Graduate Research Theses & Dissertations

With the removal and destruction of natural areas for human use, there is a push to restore natural habitats. However, most restoration activities focus on reestablishing plant communities, while less is known about the recovery of organisms in higher trophic levels and their functional roles. Considering functional traits (those that explain an organisms’ response to environmental change and impacts on ecosystems) of restored communities may reveal how communities, and the ecosystem functions they drive, recover. Of particular interest are ground beetles (Coleoptera: Carabidae) because of their diversity and important ecological roles as consumers. This study focused on the ground beetle …

Microbiome And Metagenome Of An Anthropogenic Hyperalkaline Slag-Fill Waterway In South Chicago, Illinois, Usa, Jan Ingemar Ohlsson

Graduate Research Theses & Dissertations

The aquifers and waterways around the Lake Calumet area of south Chicago, IL, USA have been affected by a century of intensive industrial waste dumping. Steel slag in particular was used as infilling to claim most of the Calumet Wetlands for industrial and residential development. Since the prohibition and cessation of these disposal activities, a growing body of research has recorded the environmental and health effects of the infill, particularly where interactions between slag minerals and rainwater produces extremely alkaline groundwater (pH ≥13). Atmospheric carbon dioxide interacting with these calcium hydroxide-rich slag fluids produces massive precipitation of a loose, white …

Computational Methods For Bicubic L1 Splines, Priyesh Koyu Rai

Graduate Research Theses & Dissertations

The past two decades have seen the development of L1 splines for interpolation and approximation of geometric data. L1 splines have been shown to preserve shapes better than the conventional L2 splines, especially for data with abrupt changes in the magnitude of spacing. Efficient computation plays an important role in the practical application of L1 splines. This thesis creates new computational methods for constructing bicubic L1 interpolating splines and bicubic L1 spline fits. The bicubic splines in this study are piecewise bicubic polynomials defined on a rectangular grid generated by the tensor product of nodes. We developed a hybrid computational …

Seabird Threats On Land And At Sea: Measuring Nearshore Response To Invasive Predator Eradications And Storm Petrel Diet Response To Fishing Expansion, Lyndsay L. Rankin

Graduate Research Theses & Dissertations

Seabirds are crucial components of many marine and island ecosystems, and yet they are the world’s most threatened group of birds. They spend most of their lives at sea, where they face numerous threats including climate change, pollution, and fisheries. When they return to land to breed, they are threatened by human disturbance and invasive species. This research examines seabird response to threats on land and at sea using stable isotope analysis. Seabirds provide multiple ecosystem services, such as depositing rich marine-derived nutrients into the islands where they nest. Invasive predators disrupt this bottom-up control by decimating seabird populations. Successful …

A Child Shall Lead Them: Exploring Discourses Of Efficacy And Climate Change As They Appear In Children's Animated Film, Jason Derry

Electronic Theses and Dissertations

Recent climate change discourse has tended to presume scientific knowledge and rational argumentation as the principle factor in convincing peoples and publics toward climate action. However, scholarship across numerous fields reveals myriad other contributing factors in how people think about and respond to this environmental crisis, which leans predominately toward silence and apathy. Alongside this, children are often centered as inheriting a calamity, yet find themselves largely disempowered. From out of this rhetorical milieu I interject by way of a multidisciplinary grounding to examine the predominate framings of efficacy in the context of children, climate change, and environmental discourse. To …

Biomass-Derived Activated Carbons For Electrical Double Layer Supercapacitors: Performance And Stress Effect, Wenxin Cao

Theses and Dissertations--Chemical and Materials Engineering

The vigorous development of human civilization has significantly increased the energy consumption in recent years. There is a great need to use renewable energy sources to substitute the depleting traditional fossil fuels, such as crude oil, natural gas and coal. The development of low-cost and high-performance energy storage devices (ESDs) and systems have drawn great attention due to their feasibility as backup power supply and their applications in portable electronics and electric vehicles. Supercapacitors are among the most important ESDs because of their long charging-discharging cycle life, high power capability and a large operating temperature range. In this thesis, high-performance …

Towards The Rational Design And Application Of Polymers For Gene Therapy: Internalization And Intracellular Fate, Landon Alexander Mott

Theses and Dissertations--Chemical and Materials Engineering

Gene therapy is an approach for the treatment of acquired cancers, infectious disease, degenerative disease, and inherited genetic indications. Developments in the fields of immunotherapies and CRISPR/Cas9 genome editing are revitalizing the efforts to move gene therapy to the forefront of modern medicine. However, slow progress and poor clinical outcomes have plagued the field due to regulatory and safety concerns associated with the flagship delivery vector, the recombinant virus. Immunogenicity and poor transduction in certain cell types severely limits the utility of viruses as a delivery agent of nucleic acids. As a result, significant efforts are being made to develop …

Development Of Novel Temperature Responsive Polymeric Sorbents And Their Applications In Water Remediation, Shuo Tang

Theses and Dissertations--Chemical and Materials Engineering

Water remediation utilizing sorption has found strong interest due to its inexpensiveness, universal nature and ease of operation. In particular, thermo-responsive sorbents consisting of N-isopropylacrylamide (NIPAAm) offer significant potential as “smart” and advanced materials to remove multiple aqueous pollutants. NIPAAm exhibits excellent thermo-responsiveness, which senses the external temperature variation and changes its swelling and sorption behaviors in a sharp and rapid manner. At the beginning of this work, an extensive review of literature has been compiled to provide a summary of NIPAAm-based thermo-responsive sorbents in water/wastewater remediation applications.

Initially, we developed a novel approach to synthesize and characterize NIPAAm copolymeric …

Functionalization Of Iron Oxide Nanoparticles And The Impact On Surface Reactive Oxygen Species Generation For Potential Biomedical And Environmental Applications, Trang Mai

Theses and Dissertations--Chemical and Materials Engineering

Iron oxide nanoparticles (IONPs) have been widely studied for a variety of applications, from biomedical applications (e.g., cell separation, drug delivery, contrast agent for magnetic resonance imaging and magnetically mediated energy delivery for cancer treatment) to environmental remediations (e.g., heavy metal removal and organic pollutants degradation). It has been demonstrated that IONPs can induce the production of reactive oxygen species (ROS) via Fenton/Haber-Weiss reactions which has been shown to be one of the key underlying mechanisms of nanoparticles toxicity. This inherent toxicity of nanoparticles has been shown to enhance the efficacy of traditional cancer therapies such as chemotherapy and radiation. …

Cell Surface Coatings For Mammalian Cell-Based Therapeutic Delivery, Pei-Jung Wu

Theses and Dissertations--Chemical and Materials Engineering

The cell plasma membrane is an interactive interface playing an important role in regulating cell-to-cell, cell-to-tissue contact, and cell-to-environment responses. This environment-responsive phospholipid layer consisting of multiple dynamically balanced macromolecules, such as membrane proteins, carbohydrate and lipids, is regarded as a promising platform for various surface engineering strategies. Through different chemical modification routes, we are able to incorporate various artificial materials into the cell surface for biomedical applications in small molecule and cellular therapeutics.

In this dissertation, we establish two different cell coating techniques for applications of cell-mediated drug delivery and the localization of cell-based therapies to specific tissues. The …

Impact Of Conformational Change, Solvation Environment, And Post-Translational Modification On Desulfurization Enzyme 2'-Hydroxybiphenyl-2-Sulfinate Desulfinase (Dszb) Stability And Activity, Landon C. Mills

Theses and Dissertations--Chemical and Materials Engineering

Naturally occurring enzymatic pathways enable highly specific, rapid thiophenic sulfur cleavage occurring at ambient temperature and pressure, which may be harnessed for the desulfurization of petroleum-based fuel. One pathway found in bacteria is a four-step catabolic pathway (the 4S pathway) converting dibenzothiophene (DBT), a common crude oil contaminant, into 2-hydroxybiphenyl (HBP) without disrupting the carbon-carbon bonds. 2’-Hydroxybiphenyl-2-sulfinate desulfinase (DszB), the rate-limiting enzyme in the enzyme cascade, is capable of selectively cleaving carbon-sulfur bonds. Accordingly, understanding the molecular mechanisms of DszB activity may enable development of the cascade as industrial biotechnology. Based on crystallographic evidence, we hypothesized that DszB …

Targeted Illumination Strategies For Hydrogen Production From Purple Non-Sulfur Bacteria, John D. Craven

Theses and Dissertations--Chemical and Materials Engineering

The movement towards a more sustainable energy economy may require not only the generation of cleaner fuel sources, but the conversion of waste streams into value-added products. Phototrophic purple non-sulfur bacteria are capable of metabolizing VFAs (volatile fatty acids)and generate hydrogen as a byproduct of nitrogen fixation using energy absorbed from light. VFAs are easily produced from dark anaerobic fermentation of food, agricultural, and municipal wastes, which could then be fed into photobioreactors of purple bacteria for hydrogen production.

The process of photofermentation by purple bacteria for hydrogen production remains attractive due to the capability of reaching high substrate conversions …

Development Of Magnetic Nanocomposite Materials As Reusable Adsorbents For Chlorinated Organics In Contaminated Water, Angela Gutierrez

Theses and Dissertations--Chemical and Materials Engineering

The constant growth in population worldwide over the past decades continues to put forward the need to provide access to safe, clean water to meet human needs. There is a need for cost-effective technologies for water and wastewater treatment that can meet the global demands and the rigorous water quality standards and at the same maximizing pollutant efficiency removal. Current remediation technologies have failed in keeping up with these factors without becoming cost-prohibitive. Nanotechnology has recently been sought as a promising option to achieve these goals. The use of iron oxide magnetic nanoparticles as nanoadsorbents has led to a new …

Nanoharvesting And Delivery Of Bioactive Materials Using Engineered Silica Nanoparticles, Md Arif Khan

Theses and Dissertations--Chemical and Materials Engineering

Mesoporous silica nanoparticles (MSNPs) possess large surface areas and ample pore space that can be readily modified with specific functional groups for targeted binding of bioactive materials to be transported through cellular barriers. Engineered silica nanoparticles (ESNP) have been used extensively to deliver bio-active materials to target intracellular sites, including as non-viral vectors for nucleic acid (DNA/RNA) delivery such as for siRNA induced interference. The reverse process guided by the same principles is called “nanoharvesting”, where valuable biomolecules are carried out and separated from living and functioning organisms using nano-carriers. This dissertation focuses on ESNP design principles for both applications. …

The Fabrication And Characterization Of Metal Oxide Nanoparticles Employed In Environmental Toxicity And Polymeric Nanocomposite Applications, Matthew Logan Hancock

Theses and Dissertations--Chemical and Materials Engineering

Ceria (cerium oxide) nanomaterials, or nanoceria, have commercial catalysis and energy storage applications. The cerium atoms on the surface of nanoceria can store or release oxygen, cycling between Ce³⁺ and Ce⁴⁺, and can therefore act as a therapeutic to relieve oxidative stress within living systems. Nanoceria dissolution is present in acidic environments in vivo. In order to accurately define the fate of nanoceria in vivo, nanoceria dissolution or stabilization is observed in vitro using acidic aqueous environments.

Nanoceria stabilization is a known problem even during its synthesis; in fact, a carboxylic acid, citric acid, is …

Oleic Acid Vesicles: Formation, Mechanisms Of Reactivity, And Uses In Determination Of Terpene Activity, Laura A. Walther

Theses and Dissertations--Chemistry

This dissertation will focus on the volatile compounds released upon the burning of incense which are numerous and varied. The first part of this dissertation is the gas chromatography-mass spectral (GC-MS) analysis of burning incense collected via solid phase microextraction (SPME) with the aim of developing a library of compounds found in incense as used in the Orthodox church.

The second part of this dissertation has the aim of developing a method for forming oleic acid bilayer vesicle membranes and a fluorescence spectroscopy method by which the reactivities of these vesicles can be analyzed. These reactivities include permeability, fluidity, aggregation, …

Illuminating Dna Packaging In Sperm Chromatin: How Polycation Lengths, Underprotamination And Disulfide Linkages Alters Dna Condensation And Stability, Daniel Kirchhoff

Theses and Dissertations--Chemistry

During spermiogenesis, somatic chromatin is remodeled and a vast majority (> 90%) of DNA histones are replaced by short arginine-rich peptides called protamines. This compaction is immense, with protamine-DNA self-assembly in sperm chromatin resulting in a final volume roughly 1/6th of a somatic nucleus. This near crystalline organization of the DNA in sperm is thought crucial both for the transport of the paternal genes as well as for the protection of genetic information as sperm chromatin is transcriptionally inactive and all DNA repair mechanisms are shut down.

Chapter 1 will include an overview of the topics discussed in this document, …