Digital Commons Network^™

A Comparison Of The Population Genetic Structure And Diversity Between A Common (Chrysemys P. Picta) And An Endangered (Clemmys Guttata) Freshwater Turtle, Scott W. Buchanan, Jason J. Kolbe, Johanna E. Wegener, Jessica R. Atutubo, Nancy E. Karraker

Biological Sciences Faculty Publications

The northeastern United States has experienced dramatic alteration to its landscape since the time of European settlement. This alteration has had major impacts on the distribution and abundance of wildlife populations, but the legacy of this landscape change remains largely unexplored for most species of freshwater turtles. We used microsatellite markers to characterize and compare the population genetic structure and diversity between an abundant generalist, the eastern painted turtle (Chrysemys p. picta), and the rare, more specialized, spotted turtle (Clemmys guttata) in Rhode Island, USA. We predicted that because spotted turtles have disproportionately experienced the detrimental effects of habitat loss …

Even Obligate Symbioses Show Signs Of Ecological Contingency: Impacts Of Symbiosis For An Invasive Stinkbug Are Mediated By Host Plant Context, Janelle Couret, Lynn Huynh-Griuffin, Ivan Antolic-Soban, Tarik Salam Acevedo-Gonzalez, Nicole M. Gerardo

Biological Sciences Faculty Publications

Many species interactions are dependent on environmental context, yet the benefits of obligate, mutualistic microbial symbioses to their hosts are typically assumed to be universal across environments. We directly tested this assumption, focusing on the symbiosis between the sap‐feeding insect Megacopta cribraria and its primary bacterial symbiont Candidatus Ishikawaella capsulata. We assessed host development time, survival, and body size in the presence and absence of the symbiont on two alternative host plants and in the insects' new invasive range. We found that association with the symbiont was critical for host survival to adulthood when reared on either host plant, with …

Divergent Caregiver And Youth Perspectives Regarding Behavioral Health Needs And Psychosocial Functioning: An Exploratory Study, Isaac Karikari, Betty Walton, Christine Marie Bishop

Faculty Publications

Background. To promote effectiveness in behavioral health treatment, the system of care framework and wraparound model accentuate inclusion of family and youth as important stakeholders, not just as consumers. This has challenged conventional practices; and youth and caregivers' perspectives have become integral to treatment planning and service delivery. This study explored caregivers and youth's perspectives of behavioral health needs and psychosocial functioning. Methods. This exploratory study utilized data collected in a Midwestern, suburban county as part of the national Child and Family Study of youth with complex behavioral health needs enrolled in the Child Mental Health Wraparound initiative. The sample …

Graduate School Of Oceanography Annual Report For Fy2019, Uri Graduate School Of Oceanography

Graduate School of Oceanography Publications

Annual report for the Graduate School Oceanography of the University of Rhode Island for the 2019 fiscal year.

Includes research projects by faculty and students, biographies of new faculty, events on the Bay campus and program development and list of donors.

Scaffold-Based Lung Tumor Culture On Porous Plga Microparticle Substrates, Aneetta E. Kuriakose, Wenjing Hu, Kytai T. Nguyen, Jyothi Menon

Biomedical and Pharmaceutical Sciences Faculty Publications

Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens—gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell …

Rapid Preparation Of Spherical Granules Via The Melt Centrifugal Atomization Technique, Yan Yang, Nan Zheng, Xiaoyue Wang, Ryan Ivone, Weiguang Shan, Jie Shen

Biomedical and Pharmaceutical Sciences Faculty Publications

Granules with superior fluidity and low moisture absorption are ideal for tableting and capsule filling. Melt granulation as a solvent-free technology has attracted increasing interest for the granulation of moisture-sensitive drugs. The objective of the present study was to develop a solvent-less and high throughput melt granulation method via the melt centrifugal atomization (MCA) technique. The granule formability of various drugs and excipients via MCA and their dissolution properties were studied. It was found that the yield, fluidity, and moisture resistance of the granules were affected by the drug and excipient types, operation temperature, and collector diameter. The drugs were …

Antagonism Of Quorum Sensing Phenotypes By Analogs Of The Marine Bacterial Secondary Metabolite 3-Methyl-N-(2′-Phenylethyl)-Butyramide, Susan M. Meschwitz, Margaret E. Teasdale, Ann Mozzer, Nicole Martin, Jiayuan Liu, Stephanie Forschner-Dancause, David C. Rowley

Biomedical and Pharmaceutical Sciences Faculty Publications

Quorum sensing (QS) antagonists have been proposed as novel therapeutic agents to combat bacterial infections. We previously reported that the secondary metabolite 3-methyl-N-(2′-phenylethyl)-butyramide, produced by a marine bacterium identified as Halobacillus salinus, inhibits QS controlled phenotypes in multiple Gram-negative reporter strains. Here we report that N-phenethyl hexanamide, a structurally-related compound produced by the marine bacterium Vibrio neptunius, similarly demonstrates QS inhibitory properties. To more fully explore structure–activity relationships within this new class of QS inhibitors, a panel of twenty analogs was synthesized and biologically evaluated. Several compounds were identified with increased attenuation of QS-regulated phenotypes, most notably N-(4-fluorophenyl)-3-phenylpropanamide against the …

Evaluating Neighborhood, Social, And Genetic Influences On Precursors Of Alcohol Use Risk Behavior In African American Adolescents, Kimberly S. Compton, Peter Barr, Katherine J. Karriker-Jaffe, Cristina Bares, Fazil Aliev, Brian Mustanski, Danielle Dick, Karen G. Chartier

Social Work Publications

Background: Using a socioecological framework, we examined neighborhood and social stressors in concert with genetic risk for alcohol dependence in relation to externalizing behaviors, important precursors to alcohol-related problems.

Methods: We used data from African American adolescents and their caregivers in the Gene, Environment, and Neighborhood Initiative, a subsample of the Mobile Youth and Poverty Study. Participants for the current analyses included 112 adolescents who reported ever having at least one full drink of alcohol. Empirical Bayes scores were used to estimate neighborhood-level violence and transitions. Multivariate models tested main effects and then interactions of family stressors, discrimination, …

Multicultural Education Professional Development: A Review Of The Literature, Hillary Parkhouse, Virginia R. Massaro, Chu Yi Lu Qiu

Teaching and Learning Publications

When their teachers are well equipped to foster inclusive and equitable classrooms, students from marginalized communities show higher rates of academic achievement, motivation, self-confidence, and self-efficacy. However, many teachers complete preparation programs feeling underprepared to work in culturally diverse classrooms, making high-quality professional development in this area crucial. We undertook a meta-ethnographic, systematic literature review of 40 studies of multicultural-education-focused professional development programs in order to better understand the forms and features of such programs that contribute to teachers’ self-efficacy and success in working with culturally diverse students. We found a small literature base with too much variation across types …

Single-Molecule Analysis Of I-Motif Within Self-Assembled Dna Duplexes And Nanocircles, Anoja Megalathan, Bobby D. Cox, Peter D. Wilkerson, Anisa Kaur, Kumar Sapkota, Joseph E. Reiner, Soma Dhakal

Chemistry Publications

The cytosine (C)-rich sequences that can fold into tetraplex structures known as i-motif are prevalent in genomic DNA. Recent studies of i-motif–forming sequences have shown increasing evidence of their roles in gene regulation. However, most of these studies have been performed in short single-stranded oligonucleotides, far from the intracellular environment. In cells, i-motif–forming sequences are flanked by DNA duplexes and packed in the genome. Therefore, exploring the conformational dynamics and kinetics of i-motif under such topologically constrained environments is highly relevant in predicting their biological roles. Using single-molecule fluorescence analysis of self-assembled DNA duplexes and nanocircles, we show that the …

Virginia Commonwealth University Graduate Bulletin [2019], Virginia Commonwealth University

VCU Bulletins

Graduate bulletin for Virginia Commonwealth University for the academic year 2019-2020. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for graduate programs.

A Preliminary Investigation Towards The Risk Stratification Of Allogeneic Stem Cell Recipients With Respect To The Potential For Development Of Gvhd Via Their Pre-Transplant Plasma Lipid And Metabolic Signature, Daniel Contaifer Jr., Catherine H. Roberts, Naren Gajenthra Kumar, Ramesh Natarajan, Bernard J. Fisher, Kevin Leslie, Jason Reed, Amir A. Toor, Dayanjan S. Wijesinghe

Pharmacotherapy and Outcomes Science Publications

The clinical outcome of allogeneic hematopoietic stem cell transplantation (SCT) may be influenced by the metabolic status of the recipient following conditioning, which in turn may enable risk stratification with respect to the development of transplant-associated complications such as graft vs. host disease (GVHD). To better understand the impact of the metabolic profile of transplant recipients on post-transplant alloreactivity, we investigated the metabolic signature of 14 patients undergoing myeloablative conditioning followed by either human leukocyte antigen (HLA)-matched related or unrelated donor SCT, or autologous SCT. Blood samples were taken following conditioning and prior to transplant on day 0 and the …

The Catalytic Effect Of D68n Mutant In Thermococcus Thioreducens Inorganic Pyrophosphatase, Zeina Sleiman

Honors Capstone Projects and Theses

No abstract provided.

Brief Behavioral Health Intervention Program For Patients With Stable Coronary Artery Disease, Chelsea Wiener

Electronic Theses and Dissertations

Healthy eating, physical activity, stress management, and smoking cessation are widely recognized as essential for preventing and treating coronary artery disease (CAD). Research on lifestyle programs for patients with CAD has largely focused on long-term interventions (e.g., several months to one-year in duration). Further, many studies have recruited patients immediately post-cardiac event. By contrast, evaluation of brief lifestyle interventions for stable patients treated in outpatient cardiology is lacking. The present study evaluated the feasibility, acceptability, and efficacy of a 3-session behavioral health lifestyle program for patients with stable CAD being treated in an outpatient cardiology clinic. Thirty-three patients were randomized …

Fabrication Of Polyelectrolyte Nanoparticles Through Hydrophobic Interaction, Ruginn Porce Catarata

Electronic Theses and Dissertations

Anticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in the core of the nanoparticle, forming a PAA shell. The nanoparticles were optimized by studying the PAA/GEM ratio and pH during fabrication. Characteristics of the nanoparticles including size, morphology …

Development Of 3d Printed And 3d Metal-Based Micro/Nanofabricated, And Nano-Functionalized, Microelectrode Array (Mea) Biosensors For Flexible, Conformable, And In Vitro Applications, Charles Didier

Electronic Theses and Dissertations

Emerging fields such as "Organs on a Chip", disease modeling in vitro, stem cell manufacturing and wearable bioelectronics are demanding rapid development of 3D Microelectrode Arrays (MEAs) for electrical interfacing with biological constructs. The work reported in this thesis focuses on two developmental tracks: "Dynamic 3D MEAs" and metal microfabrication for 3D MEAs. In the first part of the thesis, we explore the capabilities and limitations of 3D printed microserpentines (µserpentines) and utilize these structures to develop dynamic 3D microelectrodes. Analytical modeling of µserpentines flexibility followed by integration into a flexible Kapton® package and PDMS insulation are demonstrated. These 3D …

2019 Annual Report, The Jackson Laboratory

Annual Reports

No abstract provided.

Abso2luteu-Net: Tissue Oxygenation Calculation Using Photoacoustic Imaging And Convolutional Neural Networks, Kevin Hoffer-Hawlik, Geoffrey P. Luke

ENGS 88 Honors Thesis (AB Students)

Photoacoustic (PA) imaging uses incident light to generate ultrasound signals within tissues. Using PA imaging to accurately measure hemoglobin concentration and calculate oxygenation (sO₂) requires prior tissue knowledge and costly computational methods. However, this thesis shows that machine learning algorithms can accurately and quickly estimate sO₂. absO₂luteU-Net, a convolutional neural network, was trained on Monte Carlo simulated multispectral PA data and predicted sO₂ with higher accuracy compared to simple linear unmixing, suggesting machine learning can solve the fluence estimation problem. This project was funded by the Kaminsky Family Fund and the Neukom Institute.

Neurogenesis In Neurodegenerative Diseases: Role Of Mfg-E8, Cletus Cheyuo, Monowar Aziz, Ping Wang

Faculty & Staff Scholarship

Neurodegenerative diseases are devastating medical conditions with no effective treatments. Restoration of impaired neurogenesis represents a promising therapeutic strategy for neurodegenerative diseases. Milk fat globule-epidermal growth factor-factor VIII (MFG-E8) is a secretory glycoprotein that plays a wide range of cellular functions including phagocytosis of apoptotic cells, anti-inflammation, tissue regeneration, and homeostasis. The beneficial role of MFG-E8 has been shown in cerebral ischemia (stroke), neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, and traumatic brain injury. In stroke, MFG-E8 promotes neural stem cell proliferation and their migration toward the ischemic brain tissues. These novel functions of MFG-E8 are primarily mediated …

Mutualist-Provisioned Resources Impact Vector Competency, Rita V. M. Rio, Anna K. S. Jozwick, Amy F. Savage, Afsoon Sabet, Aurelien Vigneron, Yineng Wu, Serap Aksoy, Brian L. Weiss

Faculty & Staff Scholarship

ABSTRACT Many symbionts supplement their host’s diet with essential nutrients. However, whether these nutrients also enhance parasitism is unknown. In this study, we investigated whether folate (vitamin B9) production by the tsetse fly (Glossina spp.) essential mutualist, Wigglesworthia, aids auxotrophic African trypanosomes in completing their life cycle within this obligate vector. We show that the expression of Wigglesworthia folate biosynthesis genes changes with the progression of trypanosome infection within tsetse. The disruption of Wigglesworthia folate production caused a reduction in the percentage of flies that housed midgut (MG) trypanosome infections. However, decreased folate did not prevent MG trypanosomes from migrating …

Erk-Mediated Timeless Expression Suppresses G2/M Arrest In Colon Cancer Cells, Beth K. Neilsen, Danielle E. Frodyma, Jaime L. Mccall, Kurt W. Fisher, Robert E. Lewis

Faculty & Staff Scholarship

The cell cycle is under circadian regulation. Oncogenes can dysregulate circadian-regulated genes to disrupt the cell cycle, promoting tumor cell proliferation. As a regulator of G2/M arrest in response to DNA damage, the circadian gene Timeless Circadian Clock (TIMELESS) coordinates this connection and is a potential locus for oncogenic manipulation. TIMELESS expression was evaluated using RNASeq data from TCGA and by RT-qPCR and western blot analysis in a panel of colon cancer cell lines. TIMELESS expression following ERK inhibition was examined via western blot. Cell metabolic capacity, propidium iodide, and CFSE staining were used to evaluate the effect of TIMELESS …

Overcoming The School To Prison Pipeline, Revonda Slaughter

Integrated Studies

Statement of the Problem

This paper will explore the following question: How can teachers, schools and communities prevent a myriad of minority students from entering the school to prison pipeline? The exact essence of what the school to prison pipeline is, will also be analyzed within this paper. An exploration will be conducted as to why minorities are becoming the main target in the school system. This paper will give examples and explanations of why the school to prison pipeline should not be over looked.

Soil Fauna And Their Potential Responses To Warmer Soils, Bruce A. Snyder, Mac A. Callaham

Faculty and Staff Works

Soil fauna are important contributors to ecosystem functioning as well as being tremendously diverse. Effects of warming on soil fauna are understudied and complex, with multiple covariates and a mixture of direct and indirect effects. This, combined with diverse communities, results in a suite of potential responses across temporal, spatial, and biological scales. We present a conceptual diagram to relate these interacting effects and a framework for organizing and understanding past and future research in this field. Themes common in the literature include species-specificity, site-specificity, and the challenge of disentangling the connection between temperature and moisture in soils. Much extant …

Quality Assurance Report For Year 2017 Estuarine Water Quality Datasonde Monitoring, Lara Martin

PREP Reports & Publications

No abstract provided.

Understanding Electrolyte Decomposition Of Graphite/Ncm811 Cells At Elevated Operating Voltage, Nina Laszczynski, Sophie Solchenbach, Hubert A. Gasteiger, Brett L. Lucht

Chemistry Faculty Publications

Cells containing LiNixCoyMn1-x-yO2 (NCM) materials with very high nickel content suffer from cathode material degradation upon cycling, especially when operating these cells at higher voltage. While the cathode particle degradation is a problem other issues contribute to electrochemical performance loss. Graphite/LiNi0.8Co0.1Mn0.1O2 cells have been cycled to different upper cutoff voltages and investigated with electrochemical impedance spectroscopy (EIS), gas analysis with online electrochemical mass spectroscopy (OEMS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography with MS (GCMS) and inductively coupled plasma MS (ICP-MS). Increased gas evolution was observed when the upper cutoff voltage was increased from 4.2 to 4.6 V. An increase …

Degradation And Deactivation Of Bacterial Antibiotic Resistance Genes During Exposure To Free Chlorine, Monochloramine, Chlorine Dioxide, Ozone, Ultraviolet Light, And Hydroxyl Radical, Huan He, Peiran Zhou, Kyle Shimabuku, Xuzhi Fang, Shu Li, Yunho Lee, Michael C. Dodd

Civil Engineering Faculty Scholarship

This work investigated degradation (measured by qPCR) and biological deactivation (measured by culture-based natural transformation) of extra- and intracellular antibiotic resistance genes (eARGs and iARGs) by free available chlorine (FAC), NH2Cl, O3, ClO2, and UV light (254 nm), and of eARGs by •OH, using a chromosomal ARG (blt) of multidrug-resistant Bacillus subtilis 1A189. Rate constants for degradation of four 266–1017 bp amplicons adjacent to or encompassing the acfA mutation enabling blt overexpression increased in proportion to #AT+GC bps/amplicon, or in proportion to #5′-GG-3′ or 5′-TT-3′ doublets/amplicon, with respective values ranging from 0.59 to 2.3 (×1011 M–1 s–1) for …

The Prairie Post Quarterly Newsletter Of The High Plains Regional Climate Center- January 2019, Natalie Umphlett, Rezaul Mamood, Warren Pettee, Gannon Rush, Crystal J. Stiles

Prairie Post: Quarterly Newsletter of the High Plains Regional Climate Center

Inside this issue:

Message from the director........................................1

Staff spotlight...........................1

Year in review............................2

Drought THIRA toolkit...........3

Product highlight....................4

Update on regional climate conditions..................................4

Other HPRCC news.................5

Recent and upcoming travel and activities.............................6

Drug Sensitivity Screening And Targeted Pathway Analysis Reveal A Multi-Driver Proliferative Mechanism And Suggest A Strategy Of Combination Targeted Therapy For Colorectal Cancer Cells, Jinyan Shen, Li Li, Tao Yang, Niuliang Cheng, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Treatment of colorectal cancer mostly relies on traditional therapeutic approaches, such as surgery and chemotherapy. Limited options of targeted therapy for colorectal cancer narrowly focus on blocking cancer-generic targets VEGFR and EGFR. Identifying the oncogenic drivers, understanding their contribution to proliferation, and finding inhibitors to block such drivers are the keys to developing targeted therapy for colorectal cancer. In this study, ten colorectal cancer cell lines were screened against a panel of protein kinase inhibitors blocking key oncogenic signaling pathways. The results show that four of the 10 cell lines did not respond to any kinase inhibitors significantly, the other …

Construction Of A Cdna Library And Preliminary Analysis Of The Expressed Sequence Tags Of The Earthworm Eisenia Fetida (Savigny, 1826), Chang Liu, Xuan Wang, Jiu-Bo Tian, Pei-Yuan Ma, Fan-Xiu Meng, Qi Zhang, Bao-Feng Yu, Rui Guo, Zhi-Zhen Liu, Hai-Long Wang, Jun Xie, Niu-Llang Cheng, Jian-Hua Wang, Bo Niu, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Earthworms are useful indicator organisms of soil health and Eisenia fetida have been extensively used as test organisms in ecotoxicological studies. In order to gain insight into the gene expression profiles associated with physiological functions of earthworms, a full‑length enriched cDNA library of the Eisenia fetida genome was successfully constructed using Switching Mechanism at 5'End of RNA Template technology. Construction of a cDNA library and analysis of Expressed Sequence Tags (ESTs) are efficient approaches for collecting genomic information and identifying genes important for a given biological process. Furthermore, analysis of the expression abundance of ESTs was performed with the aim …

Personal Problems = Great Literature: Shelley's Motherhood Issues Reflected In Frankenstein, Campbell Ashby

Celebrating Writers and Writing in our Communities

No abstract provided.