Digital Commons Network^™

The Cure For Cultivating Fastidious Microbes, Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick, William Wischusen, Cameron Thrash

Biological Sciences Faculty Publications

Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-toextinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the …

Reliability Of Bond Strength Using Universal Adhesives To Variable Dentin Conditions, Marwa Bawazir

Student Theses, Dissertations and Capstones

Brief Background: Both three-step etch-and-rinse adhesives and universal adhesives have proven their effectiveness to produce successful bond to dentin. However, there are no studies comparing adhesives’ bond strength when applied by multiple operators, and to variable dentin conditions. Objectives: To evaluate and compare the bond strength of a universal adhesive when applied by multiple operators, and to variable dentin conditions, in comparison to three- step etch-and-rinse adhesive. Methods: Three hundred extracted human teeth were embedded in resin. Five dentists with at least ten years of experience in general dentistry and five pre-dental students applied a universal adhesive (Adhese Universal, Ivoclar …

Exposure To A Slightly Sweet Lipid-Based Nutrient Supplement During Early Life Does Not Increase The Level Of Sweet Taste Most Preferred Among 4- To 6-Year-Old Ghanaian Children: Follow-Up Of A Randomized Controlled Trial, Harriet Okronipa, Mary Arimond, Charles D. Arnold, Rebecca R. Young, Seth Adu-Afarwuah, Solace M. Tamakloe, Maku E. Ocansey, Sika M. Kumordzie, Brietta M. Oaks, Julie A. Mennella, Kathryn G. Dewey

Nutrition Faculty Publications

Background: The impact of feeding a slightly sweet nutrient supplement early in life on later sweet taste preference is unknown.

Objective: We tested the hypothesis that the level of sucrose most preferred by 4–6-y-old children exposed to a slightly sweet lipid-based nutrient supplement (LNS) early in life would not be higher than that of children never exposed to LNS.

Design: We followed up children born to women (n = 1,320) who participated in a randomized trial in Ghana. In one group, LNS was provided to women on a daily basis during pregnancy and the first 6 …

Prenatal And Postnatal Lipid-Based Nutrient Supplementation And Cognitive, Social-Emotional, And Motor Function In Preschool-Aged Children In Ghana: A Follow-Up Of A Randomized Controlled Trial, Maku E. Ocansey, Seth Adu-Afrawuah, Sika M. Kumordzie, Harriet Okronipa, Rebecca R. Young, Solace M. Tamakloe, Brietta M. Oaks, Kathryn G. Dewey, Elizabeth L. Prado

Nutrition Faculty Publications

Background: Adequate nutrition is necessary for brain development during pregnancy and infancy. Few randomized controlled trials of supplementation during these periods have measured later developmental outcomes.

Objective: Our objective was to investigate the effects of provision of prenatal and postnatal lipid-based nutrient supplements (LNS) on child development at preschool age.

Methods: We conducted a follow-up study of 966 children aged 4–6 y in 2016, born to women who participated in the International Lipid-Based Nutrient Supplements-DYAD trial conducted in Ghana in 2009–2014, representing 79% of eligible children. Women ≤20 weeks of gestation were randomized to daily LNS or …

Sex Differences In Lifestyle Behaviors Among U.S. College Freshmen, Melissa D. Olfert, Makenzie L. Barr, Camille C. Charlier, Geoffrey W. Greene, Wenjun Zhou, Sarah E. Colby

Nutrition Faculty Publications

Within lifestyle behavior research, the sex of populations causes differences in behaviors and outcomes of studies. This cross-sectional study investigated lifestyle behavior patterns in college students, examining sex differences in four areas: Nutrition, physical activity, sleep, and stress. Data from over 1100 college freshmen across 8 United States universities were used for this cross-sectional analysis. Self-reported data assessed fruit and vegetable intake, fat percent intake, physical activity, perceived stress, and sleep quality. Statistical analysis included Pearson chi-squared and Mann–Whitney’s U tests for scores by sex. Likewise, healthy cut-offs were used to determine frequency of participants within range of the five …

Uncovering The Signaling Landscape Controlling Breast Cancer Cell Migration Identifies Novel Metastasis Driver Genes, Esmee Koedoot, Michiel Fokkelman, Vasiliki-Maria Rogkoti, Marcel Smid, Iris Van De Sandt, Hans De Bont, Chantal Pont, Janna E. Klip, Steven Wink, Mieke A. Timmermans, Erik A. C. Wiemer, Peter Stoilov, John A. Foekens, Sylvia E. Le Dévédec, John W. M. Martens, Bob Van De Water

Faculty & Staff Scholarship

Ttriple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cell lines (Hs578T and MDA-MB-231) to provide a repository of signaling determinants that functionally drive TNBC cell motility. We have screened ~4,200 target genes individually and discovered 133 and 113 migratory modulators of Hs578T and MDA-MB-231, respectively, which are linked to signaling networks predictive for breast cancer progression. The splicing factors PRPF4B and BUD31 and the transcription factor BPTF are essential …

Shareholder Activism And Stakeholder Engagement Strategies: Promoting Environmental Justice, Human Rights, And Sustainable Development Goals, Erika George

Utah Law Faculty Scholarship

The United Nations 2030 Agenda for Sustainable Development is an ambitious "plan of action for people, planet and prosperity" which seeks to promote peace and eradicate poverty. The Agenda's goals cannot be reached without private sector participation and changes to certain business practices that contribute to adverse environmental and human rights impacts. When natural resources are managed responsibly the resulting economic development can help to eradicate poverty. However, when natural resources are managed poorly, certain extractives industry sector practices can generate or exacerbate human rights abuses, environmental degradation, corruption, and conflict. Fossil fuels are connected to the changing climate. The …

Caspase-11 Mediates Neutrophil Chemotaxis And Extracellular Trap Formation During Acute Gouty Arthritis Through Alteration Of Cofilin Phosphorylation, Kyle Caution, Nicholas Young, Frank Robledo-Avila, Kathrin Krause, Arwa Abu Khweek, Kaitlin Hamilton, Asmaa Badr, Anup Vaidya, Kylene Daily, Hawin Gosu, Midhun N. K. Anne, Mostafa Eltobgy, Duaa Dakhlallah, Sudha Argwal, Shady Estfanous, Xiaoli Zhang, Santiago Partida-Sanchez, Mikhail A. Gavrilin, Wael N. Jarjour, Amal O. Amer

Faculty & Staff Scholarship

Gout is characterized by attacks of arthritis with hyperuricemia and monosodium urate (MSU) crystal-induced inflammation within joints. Innate immune responses are the primary drivers for tissue destruction and inflammation in gout. MSU crystals engage the Nlrp3 inflammasome, leading to the activation of caspase-1 and production of IL-1β and IL-18 within gout-affected joints, promoting the influx of neutrophils and monocytes. Here, we show that caspase-11−/− mice and their derived macrophages produce significantly reduced levels of gout-specific cytokines including IL-1β, TNFα, IL-6, and KC, while others like IFNγ and IL-12p70 are not altered. IL-1β induces the expression of caspase-11 in an IL-1 …

Crystal Structure Of The Mitochondrial Protein Mitoneet Bound To A Benze-Sulfonide Ligand, Werner J. Geldenhuys, Timothy E. Long, Pushkar Saralkar, Toshio Iwasaki, Rasia A. A. Nuñez, Rajesh R. Nair, Mary E. Konkle, Michael A. Menze, Mark V. Pinti, John M. Hollander, Lori A. Hazlehurst, Aaron R. Robart

Faculty & Staff Scholarship

MitoNEET (gene cisd1) is a mitochondrial outer membrane [2Fe-2S] protein and is a potential drug target in several metabolic diseases. Previous studies have demonstrated that mitoNEET functions as a redox-active and pH-sensing protein that regulates mitochondrial metabolism, although the structural basis of the potential drug binding site(s) remains elusive. Here we report the crystal structure of the soluble domain of human mitoNEET with a sulfonamide ligand, furosemide. Exploration of the high-resolution crystal structure is used to design mitoNEET binding molecules in a pilot study of molecular probes for use in future development of mitochondrial targeted therapies for a wide variety …

Determinant Roles Of Dendritic Cell-Expressed Notch Delta-Like And Jagged Ligands On Anti-Tumor T Cell Immunity, Elena E. Tchekneva, Mounika U.L. Goruganthu, Roman V. Uzhachenko, Portia L. Thomas, Anneliese Antonucci, Irina Chekneva, Michael Koenig, Longzhu Piao, Anwari Akhter, Maria Teresa P. De Anquino, Parvathi Ranganathan, Nicholas Long, Thomas Magliery, Anna Valujskikh, Jason V. Evans, Rajeswara R. Arasada, Pierre P. Massion, David P. Carbone, Anil Shanker, Mikhail M. Dikov

Faculty & Staff Scholarship

Background: Notch intercellular communication instructs tissue-specific T-cell development and function. In this study, we explored the roles of dendritic cell (DC)-expressed Notch ligands in the regulation of T-cell effector function. Methods: We generated mice with CD11c lineage-specific deletion of Notch Delta-like ligand (Dll)1 and Jagged (Jag)2. Using these genetically-ablated mice and engineered pharmacological Notch ligand constructs, the roles of various Delta-like and Jagged ligands in the regulation of T-cell-mediated immunity were investigated. We assessed tumor growth, mouse survival, cytokine production, immunophenotyping of myeloid and lymphoid populations infiltrating the tumors, expression of checkpoint molecules and T-cell function in the experimental settings …

Ozark Historical Review, 2019, University Of Arkansas, Fayetteville. Department Of History

The Ozark Historical Review

No abstract provided.

As Blind As A Bat? Opsin Phylogenetics Illuminates The Evolution Of Color Vision In Bats, Bruno F. Simões, Nicole M. Foley, Graham M. Hughes, Huabin Zhao, Shuyi Zhang, Stephen J. Rossiter, Emma C. Teeling

More Faculty of Science and Engineering Research

No abstract provided.

B.S. In Speech, Language And Communication Disorders_2019-2020, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Dance And The Empathetic Mind, Evelyn Morrison

The Synapse: Intercollegiate science magazine

No abstract provided.

Redox Biology In Physiology And Disease, Matthew C. Zimmerman, Adam J. Case

Journal Articles: Cellular & Integrative Physiology

No abstract provided.

Redox-Sensitive Calcium/Calmodulin-Dependent Protein Kinase Iiα In Angiotensin Ii Intra-Neuronal Signaling And Hypertension, Urmi Basu, Adam J. Case, Jinxu Liu, Jun Tian, Yulong Li, Matthew C. Zimmerman

Journal Articles: Cellular & Integrative Physiology

Dysregulation of brain angiotensin II (AngII) signaling results in modulation of neuronal ion channel activity, an increase in neuronal firing, enhanced sympathoexcitation, and subsequently elevated blood pressure. Studies over the past two decades have shown that these AngII responses are mediated, in part, by reactive oxygen species (ROS). However, the redox-sensitive target(s) that are directly acted upon by these ROS to execute the AngII pathophysiological responses in neurons remain unclear. Calcium/calmodulin-dependent protein kinase II (CaMKII) is an AngII-activated intra-neuronal signaling protein, which has been suggested to be redox sensitive as overexpressing the antioxidant enzyme superoxide dismutase attenuates AngII-induced activation of …

Multiscale Wavelet Transformation And Amplitude Zone Time Epoch Coding For Ecg Data Extraction And Compression, S. Velmurugan, A. Mahabub Basha

Applied Mathematics & Information Sciences

Tele-health monitoring plays a vital role in healthcare applications. Electrocardiogram (ECG) signals are an essential part in diagnosis and analysis of cardiac diseases. It consumes large amount of time and space during transmission and data storage. To reduce the time and space complexity, an Integrated Multiscale Gabor Wavelet Transformation (IMAGWT) and Amplitude Zone Time Epoch Coding (IMGWT–AZTEC) mechanism is introduced. Multiscale Approximated Gabor Wavelet Transformation (MAGWT) is used in IMAGWT–AZTEC mechanism to decompose the ECG signals into many sub-bands and to extract the P, T waves and QRS complex. After extraction of signals, AZTEC and decoding techniques are introduced for …

Controlled Release Chemical Oxidation: Sodium Persulfate Encapsulated In Pvac Polymer, Mosarrat Samiha Kabir

Theses

In this study, sodium persulfate was encapsulated in polyvinyl acetate (PVAc) polymer to investigate the potential of activated persulfate to remediate total coliform and E. coli bacteria from contaminated water through a control release system. Controlled release structures were produced with the polymer and persulfate melted to form pellets at two different baking times. CRP structures containing 60 weight % sodium persulfates blended with 40 weight % polyvinyl acetate (PS- PVAc) were baked for 4 minutes and 10 minutes at 120ºC. The kinetics release of sodium persulfate was studied in a water system and in sand columns. Experimental results showed …

Feasibility Of Integrating Microfluidics Into Point-Of-Care Bio-Diagnostics, Swetha Manoharan

Theses

Potassium in the body needs to be maintained in proper levels for the normal functioning of heart and kidneys. The aim of this work is to develop a simple technique to measure the concentration of Potassium present in a bodily fluid sample, which can be used to assess the health condition of extremely sick patients, especially when the patient's condition needs to be diagnosed and results needs to be obtained rapidly under Point-of-Care settings. Microfluidics is one of the platforms used in bio-diagnostics because of several advantages such as low sample volume requirement and integration into high-throughput screening. In this …

Consumer Considerations Of Local Value-Added Dairy Products In North Carolina's Triangle, Gabriel Adjei Agyekum

Theses

The global and domestic dairy markets promise exciting opportunities for companies willing to adapt to a continuously evolving consumer market. However, local dairy farmers face a myriad of challenges as they navigate low milk prices, shrinking margins, increasing production cost, and a rising demand for plant-based varieties of milk, yogurt, cheese and other dairy-based foods. The goal of this research was to investigate consumer behavior towards value-added local dairy products in North Carolina’s Triangle cities of Raleigh, Durham, and Chapel Hill. Data were used from an overarching project with collected data from food consumers in the triangle tri-city area using …

2019-2020 Operating Budget, Morehead State University. Budget & Financial Planning Office.

Morehead State University Operating Budgets Archive

2019-2020 Operating Budget of Morehead State University.

Physician Assistant Fort Lauderdale, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Occupational Therapy Tampa, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Bachelor Of Science - Health And Wellness Coaching_2019-2020, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Teaching Out-Of-Field Internationally., Anne Price, Colleen Vale, Raphaela Porsch, Esti Rahayu, Fiona Faulkner, Máire Ní Riordáin, Cosette Crisan, Julia A. Luft

Books/Book Chapters

This chapter presents vignettes from six countries regarding the phenomenon of Teaching Across Specialisations (TAS) or as it is often known teaching out-of-field. The vignettes provide an overview of the education system and policies and practices relating to teacher education, certification, recruitment and assignment to subjects or year levels. They also provide insights into how teaching out-of-field is conceptualised, if or how it is officially reported, its extent and importantly, any local, state or national responses to teaching out-of-field. The six countries included are Australia, Germany, Ireland, the United Kingdom, the USA and Indonesia. These countries have been selected because …

Global Dynamics Of Perturbation Of Certain Rational Difference Equation, Sabina Hrustić, Mustafa R.S. Kulenović, Samra Moranjkić, Zehra Nurkanović

Mathematics Faculty Publications

We investigate the global asymptotic stability of the difference equation of the form x n+1 = Ax n2 + F/ax n2 + ex n-1 ; n = 0, 1, . . ., with positive parameters and nonnegative initial conditions such that x 0 +x -1 > 0. The map associated to this equation is always decreasing in the second variable and can be either increasing or decreasing in the first variable depending on the parametric space. In some cases, we prove that local asymptotic stability of the unique equilibrium point implies global asymptotic stability.

Improved Estimates Of Tributary Nitrogen Load To Casco Bay, Maine, Whitley Gray

Publications

Over the past two decades, total nitrogen (TN) concentrations have increased in Casco Bay (CBEP 2015). The sources of the increased nitrogen are poorly understood but occur with simultaneous population growth and land use changes. The total riverine nitrogen load to Casco Bay was previously estimated by Liebman and Milstead (2012) using the United States Geologic Survey’s (USGS) SPAtially Referenced Regression On Watershed attributes (SPARROW) model. The SPARROW model uses watershed characteristics, regional monitoring data and nitrogen source data to estimate nitrogen loading but was not validated using measurements of nitrogen in the Casco Bay watershed. This study attempts to …

Writing-To-Learn In Secondary Science Classes: For Whom Is It Effective?, Katherine Landau Wright, Tracey S. Hodges, Wendy K. Zimmer, Erin Mctigue

Literacy, Language and Culture Faculty Publications and Presentations

Although many agree that writing can make a unique contribution to learning, harnessing that contribution is difficult, particularly in the middle grades. The purpose of this study was to measure the efficacy of a feasible writing-to-learn intervention in Grade 6–11 science classes. We focused on middle school students because this group has been least responsive to writing interventions in previous research. We conducted an 8-week intervention in which students completed short metacognitive and longer argumentative writing tasks. Our results indicated that overall students’ ability to engage in scientific rhetoric improved, and we identified minimal difference in the growth of middle …

Metabolic Modulation Predicts Heart Failure Tests Performance, Daniel Contaifer Jr., Leo F. Buckley, George Wohlford, Naren G. Kumar, Joshua M. Morriss, Asanga D. Ranasinghe, Salvatore Carbone, Justin M. Canada, Cory Trankle, Antonio Abbate, Benjamin W. Van Tassell, Dayanjan S. Wijesinghe

Pharmacotherapy and Outcomes Science Publications

The metabolic changes that accompany changes in Cardiopulmonary testing (CPET) and heart failure biomarkers (HFbio) are not well known. We undertook metabolomic and lipidomic phenotyping of a cohort of heart failure (HF) patients and utilized Multiple Regression Analysis (MRA) to identify associations to CPET and HFBio test performance (peak oxygen consumption (Peak VO₂), oxygen uptake efficiency slope (OUES), exercise duration, and minute ventilation-carbon dioxide production slope (VE/VCO₂ slope), as well as the established HF biomarkers of inflammation C-reactive protein (CRP), beta-galactoside-binding protein (galectin-3), and N-terminal prohormone of brain natriuretic peptide (NT-proBNP)). A cohort of 49 patients with …

Research And Creative Activity, July 1, 2018-June 30, 2019: Major Sponsored Programs And Faculty Awards For Research And Creative Activity, University Of Nebraska-Lincoln, Office Of Research And Economic Development, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

Foreword by Bob Wilhelm, Vice Chancellor for Research and Economic Development:

This booklet highlights successes in research, scholarship and creative activity by University of Nebraska–Lincoln faculty during the fiscal year running July 1, 2018, to June 30, 2019.

It lists investigators, project titles and funding sources on major grants and sponsored awards received during the year; fellowships and other recognitions and honors bestowed on our faculty; books published by faculty; performances, exhibitions and other creative activity by our faculty; and patents and licensing agreements issued for products of Nebraska research.

2019 marks the university’s 150th anniversary, an exciting time to …