Digital Commons Network^™

New Insights Into Mycofactocin Biosynthesis, Structure And Function, Richard Selorm Ayikpoe

Electronic Theses and Dissertations

Mycofactocin is a putative ribosomally synthesized and post-translationally modified peptide (RiPP)-derived redox cofactor. Its biosynthesis is accomplished through the dedicated actions of the products of six conserved genes, mftABCDEF. The mycofactocin pathway is one of the most widely distributed RiPP systems in bacteria however, this distribution is heavily skewed towards the Mycobacteria genus including human pathogenic variants such as M. tuberculosis and M. ulcerans. Gene expression studies have demonstrated the essentiality of the pathway in the ability of M. tuberculosis to utilize the host's cholesterol as sole carbon source during latency. However, the biosynthesis, structure and physiological function …

Urease Is An Essential Component Of The Acid Response Network Of Staphylococcus Aureus And Is Required For A Persistent Murine Kidney Infection, Chunyi Zhou, Fatema Bhinderwala, Mckenzie K. Lehman, Vinai Chittezham Thomas, Sujata S. Chaudhari, Kelsey J. Yamada, Kirk W. Foster, Robert Powers, Tammy Kielian, Paul D. Fey

Journal Articles: Pathology and Microbiology

Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH3 and CO2 from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation. However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied. We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes in metabolic …

Design And Characterization Of A Consensus Hemagglutinin Vaccine Immunogen Against H3 Influenza A Viruses Of Swine, Haiyan Sun, Jung-Hyang Sur, Sarah Vitosh-Sillman, David J. Steffen, Hiep Vu

Nebraska Center for Virology: Faculty Publications

The substantial genetic diversity exhibited by influenza A viruses of swine (IAV-S) represents the main challenge for the development of a broadly protective vaccine against this important pathogen. The consensus vaccine immunogen has proven an effective vaccinology approach to overcome the extraordinary genetic diversity of RNA viruses. In this project, we sought to determine if a consensus IAV-S hemagglutinin (HA) immunogen would elicit broadly protective immunity in pigs. To address this question, a consensus HA gene (designated H3-CON.1) was generated from a set of 1,112 H3 sequences of IAV-S recorded in GenBank from 2011 to 2015. The consensus HA gene …

Backward Bifurcation, Oscillations And Chaos In An Eco-Epidemiological Model With Fear Effect, Amar Sha, Sudip Samanta, Maia Martcheva, Joydev Chattopadhyay

Journal Articles

This paper considers an eco-epidemiological model with disease in the prey population. The disease in the prey divides the total prey population into two subclasses, susceptible prey and infected prey. The model also incorporates fear of predator that reduces the growth rate of the prey population. Furthermore, fear of predator lowers the activity of the prey population, which reduces the disease transmission. The model is well-posed with bounded solutions. It has an extinction equilibrium, susceptible prey equilibrium, susceptible prey-predator equilibrium, and coexistence equilibria. Conditions for local stability of equilibria are established. The model exhibits fear- induced backward bifurcation and bistability. …

Mapping Abnormal Subcortical Neurodevelopment In A Cohort Of Thai Children With Hiv, Benjamin Wade, Benjamin Wade, Benjamin Wade, Victor Valcour, Thanyawee Puthanakit, Arvin Saremi, Boris Gutman, Talia Nir, Christa Watson, Linda Aurpibul, Kosalaraksa, Pradthana Ounchanum, Stephen Kerr, Netsiri Dumrongpisutikul, Pannee Visrutaratna, Jiraporn Srinakarin, Monthana Pothisri, Katherine Narr, Paul Thompson, Jintanat Ananworanich, Robert Paul, Neda Jahanshad

Psychology Faculty Works

Alterations in subcortical brain structures have been reported in adults with HIV and, to a lesser extent, pediatric cohorts. The extent of longitudinal structural abnormalities in children with perinatal HIV infection (PaHIV) remains unclear. We modeled subcortical morphometry from whole brain structural magnetic resonance imaging (1.5 T) scans of 43 Thai children with PaHIV (baseline age = 11.09±2.36 years) and 50 HIV− children (11.26±2.80 years) using volumetric and surface-based shape analyses. The PaHIV sample were randomized to initiate combination antiretroviral treatment (cART) when CD4 counts were 15–24% (immediate: n = 22) or when CD4 < 15% (deferred: n = 21). Follow-up scans were acquired approximately 52 weeks after baseline. Volumetric and shape descriptors capturing local thickness and surface area dilation were defined for the bilateral accumbens, amygdala, putamen, pallidum, thalamus, caudate, and hippocampus. Regression models adjusting for clinical and demographic variables examined between and within group differences in morphometry associated with HIV. We assessed whether baseline CD4 count and cART status or timing associated with brain maturation within the PaHIV group. All models were adjusted for multiple comparisons using the false discovery rate. A pallidal subregion was significantly thinner in children with PaHIV. Regional thickness, surface area, and volume of the pallidum was associated with CD4 count in children with PaHIV. Longitudinal morphometry was not associated with HIV or cART status or timing, however, the trajectory of the left pallidum volume was positively associated with baseline CD4 count. Our findings corroborate reports in adult cohorts demonstrating a high predilection for HIV-mediated abnormalities in the basal ganglia, but suggest the effect of stable PaHIV infection on morphological aspects of brain development may be subtle.

Containing The Jeremiad: Understanding Paradigms Of Anxiety In Global Climate Change Experience, Brian Glaser

English Faculty Articles and Research

This essay uses Bion’s concept of “containing” to read the psychological dynamics of jeremiads about global climate change, arguing that their structure reveals a strategy of communication that may be useful for more broadly raising awareness about this challenging state of the planet. More specifically, I argue that contemporary global climate change jeremiads have a structure that first elicits alarm and then moves to discuss solutions, and that this structure may be beneficial to those who are awakening to the reality of global climate change by rendering anxiety bearable and therefore open to purposive and creative response.

Enhancing Environmental Staff Cleaning Process And Adenosine Triphosphate (Atp), Rochelle D. Bello

Regis University Student Publications (comprehensive collection)

Clostridium difficile (C. difficile) has become the most common microbial cause of healthcareassociated infections (HAI) in U.S. hospitals and costs billions of dollars each year in excess of health care costs for acute care facilities alone (Centers for Disease Control and Prevention [CDC], 2015, para. 2). Inadequate cleaning of contaminated surfaces in the hospital environment may lead to an HAI. Potential barriers related to environmental cleaning of C. difficile infected rooms were identified at a small safety net hospital. The investigator used a convenience sample of 12 environmental staff (EVS) and a quasi-experimental pre-posttest design, to explore whether or not …

Humboldt Journal Of Microbiology: Volume 20, Number 1- May 2019, Press Humboldt

Humboldt Journal of Microbiology

No abstract provided.

A Mathematical Solution To The Sine Of Madness That Is Pharmaceutical Compulsory Licensing Under The Trips Agreement And The Doha Declaration, Ashley E. Sperbeck

Marquette Intellectual Property Law Review

A viable economic solution is necessary to address the shortcomings, textual ambiguities, and deficiencies engulfing international patent protection, leading to the inability of LDCs facing public health crises or national emergencies and lacking pharmaceutical manufacturing facilities to obtain generic pharmaceuticals. This Note poses a solution to this problem via another Amendment to the TRIPS Agreement and the Doha Declaration, which provides a mathematical framework to determine when and under what circumstances a compulsory license should be granted. Furthermore, this Note contemplates establishment of a WTO subcommittee to oversee this proposed solution and to ensure compliance with this Amendment. This concrete …

Medical Board Of California, Angela O'Hara, Debra Jorgensen

California Regulatory Law Reporter

No abstract provided.

Faith-Promoting History/ History-Promoting Faith, Martin E. Marty

Mormon Studies Review

An excerpt from a long poem by W. H. Auden comes to mind as a framework for assessing these two publications featuring Mormon historian Leonard J. Arrington. He was poised—we might say surefooted—in his Mormon faith and by commitments to the church. At the same time, Arrington consistently made clear that his calling was to be a scholar who excelled in the writing of history in modes acceptable among university-based researchers and writers of many faiths, or of non-faith. There was never any doubt that he pursued his vocation imaginatively and steadfastly through the decades. While these books give evidence …

Utah State Magazine, Winter 2019, Utah State University

Utah State Magazine

The quarterly magazine for friends and alumni of Utah State University.

Health Justice For Immigrants, Medha D. Makhlouf

Faculty Scholarly Works

Should universal health coverage include immigrants within the “universe?” Should federal taxpayers subsidize health insurance coverage for immigrants, even those who are undocumented? Should all immigrants be required to purchase health insurance? Although the Affordable Care Act (ACA) is conceived as a progressive project to expand access to coverage and promote equity in health care, it intentionally left out the 12.5 million undocumented immigrants living in the United States and preserved the existing restrictions on subsidized coverage for lawfully present non-citizens. In fact, it increased the disparity in access to health care between U.S. citizens and immigrants. As a result, …

Probiotics: Reiterating What They Are And What They Are Not, Azza A. Gadir, Raja Dhir, Gregor Reid

Microbiology & Immunology Publications

It has been over seventeen years since the scientific definition of probiotics was crafted, along with guidelines ensuring the appropriate use of the term. This definition is now used globally, yet on a consistent basis scientists, media and industry misrepresent probiotics or make generalized statements that illustrate a misunderstanding of their utility and limitations. The rate of discovery of novel organisms with potentially therapeutic benefit for both human and environmental health is progressing at an unprecedented rate. However, the term “probiotic” is often misapplied to describe any microbe with plausible therapeutic utility in the human host. It is argued that …

Penile Lymphangioma: Review Of The Literature With A Case Presentation, Mohamed Macki, Sharath K. Anand, Hayan Jaratli, Ali A. Dabaja

Urology Articles

Background: Penile lymphangiomas are rare manifestations of lymphangiomas or lymphatic malformations which are more commonly found in the head or neck region of the body. Lymphangiomas are further categorized as lymphangioma circumscriptum, cavernous lymphangioma, cystic hygroma, or acquired lymphangiomas (also known as lymphangiectasia), based on their depth and etiology.

Results: A literature review revealed only 30 cases of penile lymphangioma between 1947 and March 30, 2018. Several causes were attributed to the acquired penile lymphangiomas, including trauma, phimosis, and infection. While penile lymphangiomas can be initially mistaken for an infection, a thorough history and physical examination is sufficient to clinically …

Strengthening The Health Workforce Through The Echo Stages Of Participation: Participants' Perspectives On Key Facilitators And Barriers, Suzuho Shimasaki, Erin Bishop, Michelle Guthrie, John F Fred Thomas

Project ECHO Bibliography

INTRODUCTION: Project Extension for Community Health Outcomes (ECHO) was originally developed by the University of New Mexico's Health Science Center (UNMHSC) to build the capacities of primary-care providers and to increase specialty-care access to rural and underserved populations. ECHO Colorado, a replication site at the University of Colorado Anschutz Medical Campus (CUAMC), was developed with the same purpose and to help build the health workforce of Colorado. The CUAMC and its community-based partners recognized that by reducing unnecessary referrals to the medical campus and building primary-care capacity in communities, both would increase their scope and expand overall capacity. This study …

2019 Annual Campus Security And Fire Safety Report, University Of Nebraska-Lincoln, University Police, University Of Nebraska-Lincoln

University of Nebraska-Lincoln, University Police

2019 Annual Campus Security and Fire Safety Report, University of Nebraska-Lincoln

Safety and security information for the University of Nebraska-Lincoln, including crime and fire statistics for the 2018 calendar year, and the information required by the Drug-Free Schools and Communities Act of 1989. All data are submitted to the United States Department of Education according to law.

Integrated Microfluidic Systems With Sample Preparation And Nucleic Acid Amplification, Juxin Yin, Yuanjie Suo, Zheyu Zou, Jingjing Sun, Shan Zheng, Ben Wang, Yawei Xu, Diane C. Darland, Julia Zhao, Ying Mu

Biology Faculty Publications

Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of diseases and pathogens, yet remains a limiting factor at point of care (POC) treatment. Microfluidic systems are characterized by fast, integrated, miniaturized features which provide an effective platform for qualitative and quantitative detection of nucleic acid molecules. The nucleic acid detection process mainly includes sample preparation and target molecule amplification. Given the advancements in theoretical research and technological innovations to date, nucleic acid extraction and amplification integrated with microfluidic systems has advanced rapidly. The primary goal of this review is to outline current approaches used for …

The Rock, Fall/Winter 2019 (Vol. 88, No. 1), Whittier College

The Rock

No abstract provided.

Nutech Ventures Annual Report 2018-2019

NUtech Ventures: Publications

NUTech Ventures annual report 2018-2019.

Influence Of Side Chain Conformation On The Mechanism(S) Of Glycosylation Reactions; Investigation Of Sialidation Reaction Mechanism(S) By Kinetic Studies, Harsha Chandhana Amarasekara

Wayne State University Dissertations

Glycosylation, being the key reaction of glycobiology and carbohydrate chemistry, demands robust and stereoselective glycosylation methods. The rational development of such methods necessitates knowledge of the glycosylation reaction mechanism and the factors influencing glycosylation reactions. The work discussed in this dissertation is focused on studying glycosylation reaction mechanisms and exploring the key factors influencing them.

Chapter one reviews the background to the research, starting with a brief introduction of the significance of carbohydrates as structural and energy-storage materials. The significance of cell surface carbohydrates and their roles in communication and related processes are discussed along with examples of their applications …

Trends In Collection Of Microbiological Cultures Across Veterans Affairs Community Living Centers In The United States Over 8 Years, Haley J. Appaneal, R. Caffrey, Maria-Stephanie A. Hughes, Vrishali V. Lopes, Robin L.P. Jump, Kerry L. Laplante, David M. Dosa

Pharmacy Practice and Clinical Research Faculty Publications

Objectives: To describe and evaluate changes in the collection of microbiological cultures across Veterans Affairs (VA) Community Living Centers (CLCs) nationally.

Design: Descriptive study.

Setting: 146 VA CLCs.

Participants: We identified both positive and negative microbiological cultures collected during VA CLC admissions from January 2010 through December 2017.

Measures: We measured the average annual percentage change (AAPC) in the rate of cultures collected per 1000 bed days and per admission, overall and stratified by culture type (ie, urine, blood, skin and soft tissue, and respiratory tract). AAPCs were also calculated for the proportion and rate …

Comparative Effectiveness Of Exclusive Exposure To Nafcillin Or Oxacillin, Cefazolin, Piperacillin/Tazobactam, And Fluoroquinolones Among A National Cohort Of Veterans With Methicillin-Susceptible Staphylococcus Aureus Bloodstream Infection, Maya Beganovic, A. Cusumano, Vrishali Lopes, Kerry L. Laplante, Aisling R. Caffrey

Pharmacy Practice and Clinical Research Faculty Publications

Objective: Beta-lactam antibiotics are recommended as first-line for treatment of methicillin-sensitive Staphylococcus aureus (MSSA) bacteremia. The objective of this study was to compare effectiveness of anti-MSSA therapies among bacteremia patients exclusively exposed to 1 antimicrobial.

Method: This was a national retrospective cohort study of patients hospitalized in Veterans Affairs medical centers with MSSA bacteremia from January 1, 2002, to October 1, 2015. Patients were included if they were treated exclusively with nafcillin, oxacillin, cefazolin, piperacillin/tazobactam, or fluoroquinolones (moxifloxacin and levofloxacin). We assessed 30-day mortality, time to discharge, inpatient mortality, 30-day readmission, and 30-day S. aureus reinfection. Hazard ratios …

Proton-Pump Inhibitors Do Not Influence Clinical Outcomes In Patients With Staphylococcus Aureus Bacteremia, Aisling R. Caffrey, T. Timbrook, Syed Raza Ali, Victor Nizet, George Sakoulas

Pharmacy Practice and Clinical Research Faculty Publications

Background: Proton-pump inhibitors (PPIs) are commonly used in clinical practice for gastric acid suppression. However, these agents have also been associated with certain negative clinical outcomes. We evaluated the real-world effects of incident PPI use on clinical outcomes in patients with Staphylococcus aureus bacteremia.

Methods: This retrospective cohort study included patients admitted to Veterans Affairs hospitals with positive S. aureus blood cultures collected between 2002 and 2013 that received appropriate antibiotics within 48 hours of culture collection. Clinical outcomes among three PPI exposure groups, each compared to nonusers, were assessed with propensity-score-matched Cox proportional-hazard regression models: pretreated PPI users …

Biomedical Informatics Seminar Series (Biomiss) 2019 - 2020, Philip J. Kroth

Biomedical Informatics Seminar Series (BioMISS)

No abstract provided.

Unm Health Sciences Center Data Book 2018-19, University Of New Mexico - Health Sciences Center

Health Sciences Center Databook (1990-ongoing)

No abstract provided.

University Of New Mexico Health Sciences Center Discovery Research Annual Report 2019, Michael Haederle, Jana Fothergill, Emily Monteiro Morelli, Yamhilette Licon Muñoz, Nicole Maphis, Seth Fuller, Jett Loe, Allan Stone

Research Annual Reports

No abstract provided.

Prospects For Improving Management Of Animal Disease Introductions Using Disease-Dynamic Models, Ryan S. Miller, Kim Pepin

Zoonotics and Wildlife Disease: Publications

Management and policy decisions are continually made to mitigate disease introductions in animal populations despite often limited surveillance data or knowledge of disease transmission processes. Science-based management is broadly recognized as leading to more effective decisions yet application of models to actively guide disease surveillance and mitigate risks remains limited. Disease-dynamic models are an efficient method of providing information for management decisions because of their ability to integrate and evaluate multiple, complex processes simultaneously while accounting for uncertainty common in animal diseases. Here we review disease introduction pathways and transmission processes crucial for informing disease management and models at the …

Reverse Genetics Systems As Tools To Overcome The Genetic Diversity Of Lassa Virus, Brett Beitzel, Christine E. Hulseberg, Gustavo Palacios

United States Army: Publications

Lassa virus is endemic in a large area of sub-Saharan Africa, and exhibits a large amount of genetic diversity. Of the four currently recognized lineages, lineages I–III circulate in Nigeria, and lineage IV circulates in Sierra Leone, Guinea, and Liberia. However, several newly detected lineages have been proposed. LASV genetic diversity may result in differences in pathogenicity or response to medical countermeasures, necessitating the testing of multiple lineages during the development of countermeasures and diagnostics. Logistical and biosafety concerns can make it difficult to obtain representative collections of divergent LASV clades for comparison studies. For example, lack of a cold …

Frequent Prescribed Fires Can Reduce Risk Of Tick-Borne Diseases, Elizabeth R. Gleim

Environmental Studies / Environmental Science Faculty Scholarship

Recently, a two-year study found that long-term prescribed fire significantly reduced tick abundance at sites with varying burn regimes (burned surrounded by burned areas [BB], burned surrounded by unburned areas [BUB], and unburned surrounded by burned areas [UBB]). In the current study, these ticks were tested for pathogens to more directly investigate the impacts of long-term prescribed burning on human disease risk. A total of 5,103 ticks (4,607 Amblyomma americanum, 76 Amblyomma maculatum, 383 Ixodes scapularis, two Ixodes brunneus, and 35 Dermacentor variabilis) were tested for Borrelia spp., Rickettsia spp., Ehrlichia spp., and Anaplasma phagocytophilum. Long-term prescribed fire did not …