Digital Commons Network^™

Influences On Fungal Community Composition In Roots And Soil Of Coffee And Other Rubiaceae In Costa Rica, Elizabeth Christine Sternhagen

Environmental and Conservation Sciences

Belowground fungi interact with plants directly as pathogens and mutualists, and indirectly as nutrient cyclers, yet the factors governing fungal community composition are poorly understood. Here I examined root and soil fungi of coffee (Coffea arabica) and eight native species in the coffee family to determine 1.) whether coffee management affects guild structure of fungal communities in coffee roots and 2.) relative importance of microclimate and plant host relatedness in structuring belowground fungal communities of coffee and forest Rubiaceae. Coffee management resulted in differences in the soil environment that were associated with the richness and abundance of several fungal guilds. …

The Effect Of Zinc Oxide Nanoparticles On Plants, And On Host-Pathogen Interactions, Priyanka Deka

Environmental and Conservation Sciences

Zinc oxide nanoparticles (ZnO NPs) are a type of engineered nanomaterial that is currently being explored for use in different aspects of agriculture. So far, research on this area is limited to evaluating the phenotypical responses of plants to a high concentration of the NPs which is realistically not feasible in the actual environment. This research aims to investigate the molecular-level interactions between ZnO NPs and plants, together with another significant component of the environment, a fungal plant pathogen. Prior to studying these molecular-level interactions, the uptake of ZnO NPs in planta was validated using a fluorescent zinc ion sensor, …

Evaluating The Use Of Lottery-Based Contingency Management To Increase Physical Activity In Adults, Jennifer M. Owsiany

Graduate Theses, Dissertations, and Problem Reports (ETD)

It is widely known that physically inactive adults are at a greater risk for developing noncommunicable diseases (e.g., cancer, stroke, coronary heart disease, type 2 diabetes) and premature death compared to their physically active peers. Consequently, physical inactivity is one of the leading causes of death worldwide. Thus, it is important to develop effective ways to increase and maintain physical activity. In the current study, we randomly assigned adults between the ages of 18 and 64 years old to one of three groups (i.e., contingency management, participation-based incentive, and self-monitoring). Participants wore Fitbit Alta HR fitness tracking devices, which provided …

Kidney Ailment Prediction Under Data Imbalance, Ranaa Mahveen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic Kidney Disease (CKD) is the leading cause for kidney failure. It is a global health problem affecting approximately 10% of the world population and about 15% of US adults. Chronic Kidney Diseases do not generally show any disease specific symptoms in early stages thus it is hard to detect and prevent such diseases. Early detection and classification are the key factors in managing Chronic Kidney Diseases.

In this thesis, we propose a new machine learning technique for Kidney Ailment Prediction. We focus on two key issues in machine learning, especially in its application to disease prediction. One is related …

Development Of A Sustainability Evaluation And Control Framework For Chemical Processes, Shuyun Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

Industry, government, and society have begun to shift from economic stand-alone focus to the inclusion of sustainability in the decision-making process. This shift can be attributed to the growing environmental and social awareness that makes consumers and stakeholders not only care about product quality and cost, but also products and processes that minimize the environmental impact and conserve natural resources. As a result, some progress has been made in recognizing and understanding the challenges of sustainable development, which helps evaluating the sustainability performance of a specific process/product. In particular, sustainability assessment tools and methodologies have been developed to help the …

Quantifying Human Biological Age: A Machine Learning Approach, Syed Ashiqur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Quantifying human biological age is an important and difficult challenge. Different biomarkers and numerous approaches have been studied for biological age prediction, each with its advantages and limitations. In this work, we first introduce a new anthropometric measure (called Surface-based Body Shape Index, SBSI) that accounts for both body shape and body size, and evaluate its performance as a predictor of all-cause mortality. We analyzed data from the National Health and Human Nutrition Examination Survey (NHANES). Based on the analysis, we introduce a new body shape index constructed from four important anthropometric determinants of body shape and body size: body …

Low-Power Reconfigurable Sensing Circuitry For The Internet-Of-Things Paradigm, Alexander Dilello

Graduate Theses, Dissertations, and Problem Reports (ETD)

With ubiquitous wireless communication via Wi-Fi and nascent 5th Generation mobile communications, more devices -- both smart and traditionally "dumb" -- will be interconnected than ever before. This burgeoning trend is referred to as the Internet-of-Things. These new sensing opportunities place a larger burden on the underlying circuitry that must operate on finite battery power and/or within energy-constrained environments. New developments of low-power reconfigurable analog sensing platforms like field-programmable analog arrays (FPAAs) present an attractive sensing solution by processing data in the analog domain while staying flexible in design. This work addresses some of the contemporary challenges of low-power wireless …

Discrimination And Behavioral Responses To Communication Signals Compared Across Apteronotids., Danielle Leigh Dillon-Seeger

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory systems are often uniquely tailored to encode behaviorally relevant signals and comparative studies across species can thus reveal how evolutionary changes shape sensory functions. The structure of communication signals varies widely between ghost knifefish species. Recent findings suggest that the nervous system co-adapted to the various signal structures observed across species to support different sensory behaviors. The aim for this thesis was to compare the sensory behavior of 3 species of ghost knifefish to contrast their behavioral performance with the known differences in neurophysiology. We hypothesize that for the different signal types and species, the ability to discriminate small …

Effects Of E-Cigarette Flavoring Chemicals On Human Macrophages And Bronchial Epithelial Cells, Anna Manzi Morris

Graduate Theses, Dissertations, and Problem Reports (ETD)

E-cigarettes and vapes are relatively new devices which are popular among young adults and teens. These devices vaporize an “e-liquid” for the user to inhale, which typically contains a mixture of propylene glycol (PG), vegetable glycerin (VG), varying concentrations of nicotine, and flavoring chemicals. Due to these flavoring chemicals being intended for ingestion as a food additive, their respiratory health effects are not well understood. Thousands of e-cigarette flavors are available on the market, many of which have the potential for toxicity. The majority of e-liquid flavoring chemicals (ELFCs) have not been tested for inhalation safety. In this study, we …

Improving The Care Of Infants With Neonatal Abstinence Syndrome By Implementing Rooming In, Tammi M. Clutter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Opioid use and drug abuse has led to an increase in fetal exposure to illicit drugs in the United States, putting these infants at risk for developing neonatal abstinence syndrome (NAS). Parental involvement in the care of these infants during their hospitalization has become an essential part of the treatment process

Purpose: The purpose of this project is to implement rooming in for infants with in utero exposure to opioids as a quality improvement practice change.

Methods: A literature search relating to improving the care of infants with NAS was conducted. Educational handouts were provided to staff regarding the …

Cash For Consideration? An Examination Of Academics, Demographics, And Student Socioeconomics In The Provisioning Of Institutional Aid., Matthew G. Matyasovsky

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is an empirical investigation of student level observations of 11 cohorts of first-time full-time freshmen from a public, land-grant, research university in Appalachia. Complete with student financial aid offerings, socioeconomic status, and pre-college academic characteristics such as standardized test scores and high school GPA, I investigate the data to examine institutional behavior constrained by resource dependence. The variable of interest is institutional financial aid. Institutional financial aid awards are monetary concessions presented to students as a means to discount the overall price a student pays to enroll. As institutional aid is a direct mechanism through which the institution …

Metal Nanomaterials: Immune Effects And Implications Of Physicochemical Properties On Sensitization, Elicitation, And Augmentation Of Allergic Disease, Katherine Adair Roach

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many allergenic metals are being manufactured in nanoparticulate forms. Although the decreased size profile of metal nanomaterials has been consistently associated with more pronounced lung toxicity compared to larger materials, it is unclear if metal-induced immunotoxic effects exhibit a similar size-dependency.

The central hypothesis of these studies was that metal nanoparticles cause more pronounced immunomodulatory effects on allergic processes than larger forms of respective metals. Moreover, it was anticipated that, similar to their inflammatory potential in airways, the magnitude of these responses would correlate best with the dose metric of surface area.

The first set of studies utilized fine and …

Energy Efficiency Evaluation Of Blower Heater Non-Purge Compressed Air Dryers, Alexandra M. Botts

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are several compressed air dryers available for industrial use including, refrigerant, desiccant, and membrane. This research focuses on twin tower regenerative closed loop desiccant dryers, specifically: blower heater non-purge (BHNP) with and without cooling water pumps, Compressed-air Heater Purge (CHP), Blower Heater Purge (BHP), and Pressure Swing Heaterless (PSH). These styles of dryers are used mainly in industries that require extremely dry air such as, food manufacturing, medical air, or sensitive technology manufacturers. The research was conducted by collecting and analyzing real time current draw data on air compressors and associated dryers at eight different facilities (13 compressor systems) …

Autonomous Systems With Reuse: A Survey On The State-Of-The-Practice, Denny Laverne Hood Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

This problem report presents the results of an anonymous online survey that was used to collect information about software systems that use model-based software engineering, contain autonomy and where software reuse plays an important role. Recent advancements in computation ability and the emergence of decision-making algorithms have increased interest in and use of autonomy in applications areas such as aeronautics, automotive, military, and space industries. In these application domains, autonomy is used to reduce costs, reduce reaction times, and improve performance. Due to the emerging nature of autonomy, very little research has been done regarding the level of autonomy of …

Immunomagnetic Beads Coupled With Anti-Ph-20 Antibodies To Isolate Sperm From Dna Mixtures For Sexual Assault Kit Application, Kayla Becks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sexual Assault Kits (SAKs) account for a large portion of DNA mixture samples that are processed within a forensic laboratory, most commonly in the form of vaginal swabs. A typical vaginal swab from a SAK contains the female victim's epithelial cells and varying concentrations of sperm cells left by the perpetrator. The key to analyzing these types of samples is to separate the mixture into male and female fractions. As such, there is a demand to develop novel techniques that have the capability of recovering as much of the male fraction as possible, specifically in cases where there is a …

Artificial Immune System For Unmanned Aerial Vehicle Abnormal Condition Detection And Identification, Ryan G. Mclaughlin

Graduate Theses, Dissertations, and Problem Reports (ETD)

A detection and identification scheme for abnormal conditions was developed for an unmanned aerial vehicle (UAV) based on the artificial immune system (AIS) paradigm. This technique involves establishing a body of data to represent normal conditions referred to as “self” and differentiating these conditions from abnormal conditions, referred to as “non-self”. Data collected from simulation of the UAV attempting to autonomously fly a pre-decided trajectory were used to develop and test a scheme that was able to detect and identify aircraft sensor and actuator faults. These faults included aerodynamic control surface locks and damages and angular rate sensor biases. The …

Richwood, West Virginia After The 2016 Flood: Place, Devastation, And Hope In An Appalachian Community, Christine Elizabeth Witt

Graduate Theses, Dissertations, and Problem Reports (ETD)

How does a community cope with a crisis that threatens its identity or even its existence? This is the question facing the town of Richwood, West Virginia, after a devastating flood that impacted much of the town in 2016. Some of the consequences of the 2016 flood were the loss of the high school building, followed by difficulties receiving the FEMA (Federal Emergency Management Agency) money to rebuild the school, and a loss of additional FEMA money for other critical issues due to alleged corruption. How do community residents cope emotionally with devastation? How do they understand the causes of …

The Politics Of Drug Courts, Jeffrey Chris Moss

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examined drug courts from a public policy and political science perspective. The first portion of the study focused on the history of sentencing policy from the 1970s through the drug court movement. The second chapter addressed gaps in the policy literature about how drug courts were created and how they evolved. Another focal point was determining how state-level actors such as legislators, state supreme courts, and bureaucratic agencies regulated drug court policy in each particular state. From this data, a continuum was formed to determine which states operated from a top-down management style for drug courts and which …

The Acute Effect Of Propanil On Reproductive Hormones, Ovarian Gene Expression And Splenic Cells In C57bl/6 Female Mice, Malia Danielle Berg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Earlier evidence indicates exposure to propanil, a post-emergent herbicide used on rice and wheat fields, increases the number of antibody-secreting cells (ASC) in the spleen of mice after immunization with heat-killed Streptococcus pneumoniae (HKSP, 2x10⁸ CFU). Interestingly, an intact hypothalamic-pituitary-gonadal (HPG) axis is required for this immune response assessed on day 7-10 after immunization in female mice. Hence, an overarching premise is that propanil arbitrates the connection between the reproductive and immune system by altering the concentration of hormones involved in the HPG axis, which may then affect the populations of splenic cells. Hypotheses were tested with C57Bl/6 female …

Three Essays On Water Economics, Fahad Alzahrani

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation consists of three essays that explore the impacts of changes in public water quality, reliability, and raw water source. The first essay explores the relationship between episodes of contaminated drinking water and health care expenditures in the United States. Using panel data from 48 contiguous states from 2000 to 2011 and spatial econometrics modeling methods, the results indicate that there is positive and statistically significant impact on per capita health care expenditures from the percentage of a state’s population exposed to contamination episodes. The second essay examines the problem of water supply reliability due to aging infrastructure of …

Critical Physicochemical Properties For Nanoparticle Toxicity: Impact Of Surface Coating And Size On Particle-Induced Cell Transformation And Inflammatory Response, Tiffany Kornberg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoparticles, which measure 100 nm in at least one dimension, have surged in development, production, and use for a wide range of applications. However, the rapid pace of development for these emerging materials with unclear/unknown toxicity profiles makes it difficult to adequately assess health risk associated with exposure. One critical obstacle which limits scientific research to fill these critical knowledge gaps is the lack of accurate and predictive models for nanotoxicology studies, particularly those which involve occupationally relevant exposure scenarios (pulmonary exposure to low dose of particles in the circulating air). Typically, animal models are used to assess potential systemic …

Examining The Intersection Of Gender And Age In Victim Blaming, Jerin Lee

Graduate Theses, Dissertations, and Problem Reports (ETD)

Victim blaming refers to an attributional tendency in which some level of responsibility for a negative outcome is placed on victims (Maes, 1994). Many victims of crimes face stigmatization in the form of blame from friends, acquaintances, the criminal justice system, media, strangers, and even perpetrators of the crimes themselves (e.g., Cross, Parker, & Sansom, 2019; Gordon & Riger, 1991). Victim characteristics, type of crime, and observer characteristics all influence victim blaming tendencies. However, no studies to date have tested whether these factors in combination elicit differential reactions to victims of crimes. The present research tested how the intersection of …

Chemometric Calibration Of Surface Enchanced Raman Spectra For Quantitative Analysis, Xiaofei Shi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Raman spectroscopy, recognized as a powerful analytical technique, has been widely employed in many fields especially in the detection of hazard material containing in food or water. However, multiplexed analyte quantification based on Raman spectra remains a challenge due to the difficulties in accurate and precise modeling of the relationship between multiple analyte concentrations and dense spectral data with noise.

In this work, a statistical procedure was developed to efficiently generate high-quality calibration models quantifying the analyte concentrations versus spectra relationship. The resulting calibration models are able to provide estimated concentration ranges (which reflect both point and uncertainty estimates) for …

Pain Relief Knowledge, History, And Expectations Among Emergency Dental Patients, Daniel C. Brawley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction

Dentists frequently treat and manage patients with acute dental pain. Patients often seek care to treat their pain. As a part of that treatment, it is common for providers to use both prescription and non-prescription medications in the management of the patient’s dental pain. This study focused on understanding the patient’s knowledge, history and expectations regarding prescription and non-prescription medication in the management of their dental pain.

Methods

A self-report survey was developed, using Likert-type scales, consisting of 32 items, including standardized, psychometrically-tested instruments. The survey was administered to 108 adult outpatients who reported to the West Virginia University …

Psychological Trauma And Resilience Of Police Officers Involved In An Ambush: An Exploratory Study, Erin A. Teaff

Graduate Theses, Dissertations, and Problem Reports (ETD)

Law enforcement officers are widely recognized as having one of the most dangerous and stressful occupations, which can lead to lasting physical and psychological impacts. While research has examined the psychological trauma and resilience experienced by police officers, there have been very few research studies involving ambushes. Due to the continued risk of ambushes on police officers, gaining insight into the trauma and resilience of officers during these types of situations would be beneficial in providing psychologists with information to aid in their treatment officers involved in an ambush and similar unprovoked attacks. The primary aim of this study was …

Promoting Cognitive And Fine Motor Development In The Art Room For Young Students With Exposure To Trauma: An Exploratory Case Study., Tiffany Marie Minuci

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite the overwhelming research that suggests visual artmaking experiences can produce positive outcomes for individuals, many state art education policies fail to implement and integrate the arts into core academic curriculums. Specifically in the state of West Virginia, kindergarten through twelfth grade students often lack the access to a quality and consistent art education. With high teacher-pupil ratios and inclusive learning environments, educators face the difficult task of meeting all the individual needs of their students’ in the classroom. This not only affects whole student populations, but also poses risks for children who have been exposed to trauma. Traumatic experiences …

Identity Formation Of Adolescents Who Are Homeschooled: Mothers' Perceptions, Peggy Joan Mcqueen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Homeschooling is a growing educational choice with very little research about the psycho-social outcomes associated with the process. This qualitative study sought to examine the potential facilitative benefit of homeschooling upon the formation of identity. The participants were 26 mothers of homeschooled adolescents in Pennsylvania. Parent responses were examined using thematic analysis. Constituent aspects of parental motivation and methodology were investigated to assess the presence of elements facilitative in the process of identity formation as identified by Erik Erikson (1959, 1963). In examining methods used in homeschooling, it was found that the mothers in this study are providing the support …

Assessment Of Fracture Resistance Of Maxillary First Molars After Root Canal Preparation Using Three Different Rotary Instruments (V-Taper, Protaper, Vortex Blue): Finite Element Analysis, Mehran Malakpour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Long term success of endodontically treated teeth has been one of the most challenging aspects of the field of endodontics due to reduced fracture resistance of these teeth. Recently, researchers have heightened focus on conservative endodontics in order to sustain the tooth integrity, and reduce factors impacting the fracture resistance of endodontically treated teeth. As a result, rotary instrument manufacturers have developed files that have a minimal effect on original root canal anatomy. This study used Finite Element Analysis to investigate the dental biomechanical effect of rotary instrumentation during root canal therapy. The aim of this study is to …

Multidimensional Analysis Of Vulnerability: Methodological Advances And A Case Study From Malawi., Park Mcmillan Muhonda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Since 1990s rural households in Malawi, constituting 85% of the population, have experienced deepening livelihood vulnerability, often manifested as persistent food insecurity. Livelihood crises have since been blamed on or attributed directly to weather perturbations/climatic shocks i.e. El-Nino induced climate variability/drought conditions. This study revealed that persistent livelihood crisis in rural Malawi cannot be attributed to or squarely blamed on weather shocks alone, rather it is at the intersection of various livelihoods shocks that rural livelihood vulnerability in Malawi is exacerbated i.e. worsening and deepening.

Thus, rural livelihood vulnerability to climate shocks in Malawi is manifest not in isolation but …

Behavioral Impairment Following Gestational Exposure To Titanium Dioxide Nanomaterial Aerosols In Male And Female Rats, Matthew Leland Eckard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Engineered nanomaterials (ENMs) are beginning to be recognized as hazardous to human and animal health. Titanium dioxide (TiO2) is primarily used as a whitening agent in paints, plastics, and sunscreens. While relatively inert in its bulk form, nano-TiO2 (diameter) can produce prolonged inflammation and oxidative stress in target tissue. Recently, the potential for nano-TiO2 to cause neuroinflammation and damage has heightened concerns about its continued use. One important concern is that nano-TiO2, and other metal oxide ENMs, may affect fetal neurodevelopment. Accordingly, it is imperative to screen ENMs, like TiO2, for possible neurotoxicity following developmental exposures. The current set of …