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Age And Growth Of Hardhead Catfish And Gafftopsail Catfish In Coastal Louisiana, Usa, Shane Flinn, Stephen Midway

Faculty Publications

The Hardhead Catfish Ariopsis felis and Gafftopsail Catfish Bagre marinus are marine catfishes that have low recreational and commercial landings in U.S. waters, although they are among the most abundant species in coastal waters of the Gulf of Mexico. Few biological studies exist on marine catfishes, with most studies focusing on their unique reproductive biology. This study evaluated weight-length relationships for Gafftopsail Catfish (n = 264) and Hardhead Catfish (n = 823) sampled from across coastal Louisiana, USA, during 2016-2018; subsamples (93 and 266, respectively) were aged using lapilli for age and growth analyses. Hardhead Catfish showed a small but …

Connecting The Molecular Function Of Micrornas To Cell Differentiation Dynamics., Russell Posner, Reinhard Laubenbacher

Faculty Research 2019

MicroRNAs form a class of short, non-coding RNA molecules which are essential for proper development in tissue-based plants and animals. To help explain their role in gene regulation, a number of mathematical and computational studies have demonstrated the potential canalizing effects of microRNAs. However, such studies have typically focused on the effects of microRNAs on only one or a few target genes. Consequently, it remains unclear how these small-scale effects add up to the experimentally observed developmental outcomes resulting from microRNA perturbation at the whole-genome level. To answer this question, we built a general computational model of cell differentiation to …

Family Law's Coldest War: The Battle For Frozen Embryos And The Need For A Statutory White Flag, Mary Joy Dingler

Seattle University Law Review

Without concrete legislative guidance, courts are left to a variety of unsatisfactory methods of determining the disposition of frozen embryos in dissolutions and custody disputes. Beginning in 1992, courts have been issuing problematic rulings that are reached through the application of three approaches: (1) the balancing-interests test; (2) the contemporaneous mutual consent approach; and (3) the contractual approach. These approaches are examined in this Comment through the lens of selected cases and the largely inequitable outcomes for parties are critiqued. Courts even lament the lack of statutory guidance in deciding these disputes but are resigned to employing these largely flawed, …

Sequencing Heparan Sulfate Using Hilic Lc-Netd-Ms/Ms, Jiandong Wu, Juan Wei, Pradeep Chopra, Geert Jan Boons, Cheng Lin, Joseph Zaia

Chemical and Biochemical Engineering Faculty Research & Creative Works

Heparan Sulfate (HS) Mediates a Wide Range of Protein Binding Interactions Key to Normal and Pathological Physiology. Though Liquid Chromatography Coupled with Mass Spectrometry (LC-MS) based Disaccharide Composition Analysis is Able to Profile Changes in HS Composition, the Heterogeneity of Modifications and the Labile Sulfate Group Present Major Challenges for Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Sequencing of the HS Oligosaccharides that Represent Protein Binding Determinants. Here, We Report Online LC-MS/MS Sequencing of HS Oligosaccharides using Hydrophilic Interaction Liquid Chromatography (HILIC) and Negative Electron Transfer Dissociation (NETD). a Series of Synthetic HS Oligosaccharides Varying in Chain Length (Tetramers and Hexamers), …

Usft Prog Proposal Cas Cell Bio Microbio Molecular Bio Cell Molecular Bio Bs 20190913, Usf Libraries

All Assessment Data

No abstract provided.

9th Annual Postdoctoral Science Symposium, University Of Texas Md Anderson Cancer Center Postdoctoral Association

Annual Postdoctoral Science Symposium Abstracts

The mission of the Annual Postdoctoral Science Symposium (APSS) is to provide a platform for talented postdoctoral fellows throughout the Texas Medical Center to present their work to a wider audience. The MD Anderson Postdoctoral Association convened its inaugural Annual Postdoctoral Science Symposium (APSS) on August 4, 2011.

The APSS provides a professional venue for postdoctoral scientists to develop, clarify, and refine their research as a result of formal reviews and critiques of faculty and other postdoctoral scientists. Additionally, attendees discuss current research on a broad range of subjects while promoting academic interactions and enrichment and developing new collaborations.

Cobalt(Ii) Diphenylazodioxide Complexes Induce Apoptosis In Sk-Hep-1 Cells, Norah J. Alghamdi, Lakshmi Balaraman, Kylin A. Emhoff, Ahmed M.H. Salem, Ruhan Wei, Aimin Zhou, W. Christropher Boyd

Chemistry Faculty Publications

The cobalt(II) complex salts [Co(bpy)(az)₂](PF₆)₂ and [Co(az)₄](PF₆), each bearing the unusual cis-N,N'-diphenylazodioxide ligand, were both screened as possible anticancer agents against SK-HEP-1 liver cancer cells. Both compounds were found to induce substantial apoptosis as an increasing function of concentration and time. Measurement of apoptosis-related proteins indicated that both the extrinsic and intrinsic pathways of apoptosis were activated. The apoptotic activity induced by these salts is not displayed either by simple cobalt(II) salts or complexes or by the free nitrosobenzene ligand. Additionally, these compounds did not induce apoptosis, as assessed by poly(adenosine …

The Porn Crisis: This Generations Sexual Outlet, Daniela Williams

Pop Culture Intersections

With the development of technology, we opened a world, not only to new ideas and innovations but also to the world of pornography. Pornography has invaded our culture, in music, television shows, movies even social media. Advocates for the production and consumption of pornography claim pornography to be a form of self-expression that is healthy and essential to discovering their sexual experiences. However, this paper argues that the lack of regulation is contributing to the damaging effects pornography has on the consumers’ psyche and behavior, it is also factoring into our society’s decline of public health. This claim is supported …

Trypan Blue Exclusion Assay Verifies In Vitro Cytotoxicity Of New Cis-Platinum (Ii) Complex In Human Cells, Rajaa A. Hussein, Azhar Jasim Mohsin

Baghdad Science Journal

Various assays are used to determine the toxic effects of drugs at cellular levels in vitro. One of these methods is the dye exclusion assay, which measures membrane integrity in the presence of Trypan blue. Trypan blue the dye which was used in this study to investigate cytotoxic effect of a new Cis –dichloroplatinum (II) complex [(Qu)2PtCl2] on the viability of polymorphonuclear cells (PMNs). Three concentrations of platinum complex were prepared (70, 35and 17.5 µg/ ml) and the results revealed that the percentage of cell viability decreased as the platinum complex concentration increased in comparison with control. The platinum complex …

Implementing And Evaluating A Course-Based Undergraduate Research Experience (Cure) At A Hispanic-Serving Institution, Michele I. Shuster Phd, Jennifer Curtiss, Timothy F. Wright, Camilla Champion, Maryam Sharifi, Judith Bosland

Interdisciplinary Journal of Problem-Based Learning

We are examining the impact of a course-based undergraduate research experience (CURE) at a land-grant, Hispanic-serving institution in the southwestern United States. Students in our CURE completed one or two extended research projects over a single semester. Our CURE enrolled a high proportion of underrepresented minority students (70.3%), including 60.2% Hispanic students. One year after CURE completion, 31.5% of CURE students had graduated with a STEM degree, and 54.3% were enrolled in a STEM major. Pre- and postcourse surveys of indicators of persistence including scientific self-efficacy, scientific identity, valuing scientific community objectives, and intention to persist showed positive shifts. Impacts …

Medical School Watercooler Newsletter - August 25, 2019, Lindsey Lyle

Watercooler Newsletter

This is the August 25, 2019 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.

Contents Include:

USA medical students named to Alpha Omega Alpha Honor Medical Society

Macaluso named professor and chair of microbiology and immunology at USA College of Medicine

Clyde G. Huggins Award for Summer Research winners announced

Mark Your Calendar: Randall W. Powell Endowed Memorial Lectureship

Block party for a cause: increasing vaccination rates in rural Alabama

USA Health welcomes new interventional cardiologist

Synthetic Studies On Small Molecule Modulators Of Src Homology 2 (Sh2) Domain-Containing Inositol 5’-Phosphatase (Ship), Otto Milhous Dungan

Dissertations - ALL

Small molecule modulators of SH2-containing inositol 5’-phosphatase (SHIP) have recently become a hotly pursued area in medicinal chemistry. Pharmaceutical targeting of SHIP with small molecules has been identified as a new method to directly influence the phosphoinositide 3-kinase (PI3K) cell signaling pathway. Modulation of this pathway may be utilized in the development of interesting new therapeutics. A better understanding of the role that SHIP phosphatase activity plays in a number of disease states ranging from cancer to autoimmune disease to obesity may be ascertained by use of small molecule inhibitors. These investigations involved the synthesis of aminosteroid inhibitors and a …

Usft Prog Proposal Cas Integrative Bio Marine Biology Bs 20190822, Usf Libraries

All Assessment Data

No abstract provided.

Wyatt Lab Gets Another Ride To Space Station As Nasa Preps For Moon Mission, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Commercial Fishing Livelihoods, Permit Loss, And The Next Generation In Bristol Bay, Alaska, Jesse M. Coleman

Fisheries

Fishing people across the globe have experienced a fundamental restructuring of their livelihoods, communities, and economies as the result of shifts to rights-based fisheries management in the past halfcentury. The ideological underpinnings of this movement are based in neoliberalism, which is a belief system that values individualism, competition, private property, and governance by the free market. I examine some of the long-term and latent effects of this and other significant historical transitions in the fishery-dependent Bristol Bay region of Alaska. Relationships between humans and salmon in Bristol Bay evolved over thousands of years and inform the way that many fishing …

Comparative Transcriptomic Analysis And Structure Prediction Of Novel Newt Proteins, Abijeet Singh Mehta, Agustin Luz-Madrigal, Jian-Liang Li, Panagiotis A. Tsonis, Amit Singh

Biology Faculty Publications

Notophthalmus viridescens (Red-spotted Newt) possess amazing capabilities to regenerate their organs and other tissues. Previously, using a de novo assembly of the newt transcriptome combined with proteomic validation, our group identified a novel family of five protein members expressed in adult tissues during regeneration in Notophthalmus viridescens. The presence of a putative signal peptide suggests that all these proteins are secretory in nature. Here we employed iterative threading assembly refinement (I-TASSER) server to generate three-dimensional structure of these novel Newt proteins and predicted their function. Our data suggests that these proteins could act as ion transporters, and be involved …

On The Fabrication And Development Of Tissue On A Chip Devices With Included Perfused Vasculatures, Usama Ismail

McKelvey School of Engineering Graduate Student Theses & Dissertations

The advent of Tissue-on-a-Chip (TiOC) devices has provided a novel way for researchers to approach biological study and drug development. As a platform that enables human in vivo conditions to be accurately replicated ex vivo, TiOC can accelerate both fundamental biological research to answer basic questions regarding tissue behavior and function (e.g., cell-cell interactions in the tumor microenvironment (TME)) and translational research that includes testing of standard and novel therapeutics. The reported work focuses on development of processes and technologies common to two classes of TiOC. The first device is a single-layer, multi-compartment microfluidic device for investigation of pancreatic …

Integrated Pest Management In The Academic Small Greenhouse Setting: A Case Study Using Solanum Spp. (Solanaceae)., Daniel S. Hayes, Ingrid E. Jordon-Thaden, Jason T. Cantley, Angela J. Mcdonnell, Christopher T. Martine

Faculty Journal Articles

Premise

Botanical faculty and staff at academic institutions are often tasked with establishing and/or caring for plant collections held in small greenhouse facilities. Once plants are in place, an especially acute challenge is managing plant pest/pathogen populations. Integrated pest management (IPM) approaches are an excellent option, but few examples exist in the literature of successful programs that have been developed in academic small greenhouse settings.

Methods and Results

Over several years, we developed an IPM program for two small research greenhouses on the campus of a primarily undergraduate institution where hundreds of plants have been grown for studies in the …

Adult And Juvenile Rats Differentially Express Mpfc Glua2 Following Traumatic Memory Retrieval, Edgar Rodriguez

Theses and Dissertations

To advance our understanding of how traumatic memories are modulated between adults and juveniles, we characterize the AMPAr subunits, GluA1, GluA2, GluA3 in the mPFC of rodents following traumatic memory retrieval.

GluA2 was differentially expressed in adults but not juveniles rats. There were no changes in GluA1 or GluA3 expression

Chemiluminescence Imaging Probes For Quantification Of Hno And Ph, Weiwei An

Chemistry Theses and Dissertations

Chemiluminescence is the light emission from a chemical reaction. The excited state is accessed in the course of a chemical reaction and relaxes to the ground state with the emission of a photon. Independence from the need for light excitation gives chemiluminescence several advantages over fluorescence including better signal-to-noise ratio and reduced background at deeper tissue imaging depth. As a result, the sensitivity of chemiluminescent probes for biological analyte detection has been improved significantly. Because of the sensitivity of chemiluminescent probes, we are planning to use this technique to quantify important factors for biological activities.

In this dissertation, two quantification …

The Database Of Identified Root-Specific Genes And Their Promoters In Maize, Sorghum, And Soybean, Gleb Moisseyev, Divith Rajagopal, Alix Cui, Kiyoul Park, Edgar B. Cahoon, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Root genes are essential to plants as they dictate factors such as the strength of the plant, reproductivity success, etc. However, in the status quo, studies on root genes are simply ineffective. To be more specific, currently, there are very few online databases of root genes and promoters, which essentially deters root gene studies from being successful. To fix this problem, our lab constructed and coded an online database that contains information about the roots of maize, soybean, and sorghum. We collected 1200 root genes and assessed the strength and success of a given gene. This online database of these …

Development Of A Low Profile, Endoscopic Implant For Long Term Brain Imaging, Benjamin Scott Kemp

Doctoral Dissertations

The increased public awareness of concussion and traumatic brain injury has motivated continued research into the brain, its functions, and especially its response to injury, with a focus on improving the brain’s repair capabilities. However, due to the critical nature of the tissue, it is currently difficult for researchers to acquire high resolution images below the cortex without sacrificing a lab animal. Sacrificing an animal greatly reduces the amount of data that can be obtained from it, making longitudinal studies unappealing or unfeasible because a large number of animals is needed to obtain useful data over multiple time points. Additionally, …

On The Measurement Of Energy Dissipation Of Adhered Cells With The Quartz Microbalance With Dissipation Monitoring, Amir Monemian Esfahani, Weiwei Zhao, Jennifer Y. Chen, Changjin Huang, Ning Xi, Jun Xi, Ruiguo Yang

Department of Mechanical and Materials Engineering: Faculty Publications

We previously reported the finding of a linear correlation between the change of energy dissipation (ΔD) of adhered cells measured with the quartz crystal microbalance with dissipation monitoring (QCM-D) and the level of focal adhesions of the cells. To account for this correlation, we have developed a theoretical framework for assessing the ΔD-response of adhered cells. We rationalized that the mechanical energy of an oscillating QCM-D sensor coupled with a cell monolayer is dissipated through three main processes: the interfacial friction through the dynamic restructuring (formation and rupture) of cell-extracellular matrix (ECM) bonds, the interfacial viscous damping by the liquid …

The Influence Of Mir-322 On Skeletal Muscle Differentiation, Miles Alexander Soyer

Legacy Theses & Dissertations (2009 - 2024)

Skeletal muscle plays a crucial role in coordinating voluntary movement and accounts for nearly 50% of total body mass. Dysregulation in skeletal muscle development is known to cause muscle degenerative diseases including the devastating Duchenne Muscular Dystrophy (DMD). The majority of the biological studies investigating muscle development were based on myogenic transcription factors and signaling molecules including: Pax7, Myf5, MyoD, WNT, TGF-β and BMP. After the discovery of non-coding RNAs including microRNAs, it was postulated that these molecules could regulate gene expression and thus affect differentiation and development. MicroRNAs are small non-coding RNAs (~17-25 nucleotides) that regulate gene expression negatively …

The Transcriptome Of The Veiled Chameleon (Chamaeleo Calyptratus): A Resource For Studying The Evolution And Development Of Vertebrates, Brendan John Pinto, Daren C. Card, Todd A. Castoe, Raul E. Diaz Jr., Stuart V. Nielsen, Paul A. Trainor, Tony Gamble

Biological Sciences Faculty Research and Publications

Purpose

The veiled chameleon (Chamaeleo calyptratus) is an emerging model system for studying functional morphology and evolutionary developmental biology (evo‐devo). Chameleons possess body plans that are highly adapted to an arboreal life style, featuring laterally compressed bodies, split hands/ft for grasping, a projectile tongue, turreted independently moving eyes, and a prehensile tail. Despite being one of the most phenotypically divergent clades of tetrapods, genomic resources for chameleons are severely lacking.

Methods

To address this lack of resources, we used RNAseq to generate 288 million raw Illumina sequence reads from four adult tissues (male and female eyes and gonads) …

Synthesis And Biological Studies Of Amphiphilic Compounds Derived From Saccharides And Aminoglycosides, Madher N. Alfindee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Adjacent cells communicate through gap junctions (GJs). These GJs are formed by head to head docking of two hemichannels (HCs) from two adjacent cells. HCs are connexin hexamer proteins. Connexin mutation is the most frequent cause of childhood hearing loss. This hearing impairment affects 2 in every 2000 children. Inhibition of the HCs might be the key factor to treat such disorders. A library of amphiphilic kanamycins was synthesized to be tested as HC inhibitors. These compounds showed excellent inhibition activity in comparison with the parent compound (kanamycin A) with less toxicity.

A library of monosaccharide esters with varying carbon …

Synthesis And Characterization Of Gd-Doped Inp/Zns Quantum Dots For Use In Multimodal Imaging Probes, Molly Erin Duszynski

Graduate Theses/Dissertations

Quantum dots (QDs), which are intensely fluorescent nanocrystals ranging 2-10 nanometers in diameter, have shown promise in fluorescence imaging. However, in vivo applications of QDs are limited due to the opaque surrounding of tissue and bones. In this study, InP/ZnS QDs were doped with a paramagnetic atom in an attempt to render them MRI-active. We have further bioconjugated these nanoprobes to develop highly specific MRI-active probes that can be used for detection of neurodegenerative diseases. These bioconjugated nanoprobes detect a mutated form of alpha-synuclein that forms oligomers that are a hallmark of Parkinson’s disease andother alpha-synucleinopathies. Here, we have optimized …

Designing And Sample Size Calculation In Presence Of Heterogeneity In Biological Studies Involving High-Throughput Data., Sudhir Srivastava

Electronic Theses and Dissertations

The designing and determination of sample size are important for conducting high-throughput biological experiments such as proteomics experiments and RNA-Seq expression studies, thus leading to better understanding of complex mechanisms underlying various biological processes. The variations in the biological data or technical approaches to data collection lead to heterogeneity for the samples under study. We critically worked on the issues of technical and biological heterogeneity. The quantitative measurements based on liquid chromatography (LC) coupled with mass spectrometry (MS) often suffer from the problem of missing values (MVs) and data heterogeneity. We considered a proteomics data set generated from human kidney …

Does Thermotolerance In Daphnia Depend On The Mitochondrial Function?, Rajib Hasan

Electronic Theses and Dissertations

Thermotolerance limit in aquatic organism is set by the ability to sustain aerobic scope to sudden temperature shifts. This study tested the genetic and plastic differences in thermotolerance of Daphnia that can be explained by the differences in the ability to retain mitochondrial integrity at high temperatures. Five genotypes with different biogeographic origins were acclimated to 18^ᵒC and 25^ᵒC. We developed a rhodamine 123 in-vivo assay to measure mitochondrial membrane potential and observed higher fluorescent in heat damaged tissues as the disruption of the mitochondrial membrane potential. Significant effects on temperature tolerance were observed with CCCP …

Shallow- Water Hardbottom Communities Support The Separation Of Biogeographic Provinces On The West- Central Florida Gulf Coast, Shelby Eagan

HCNSO Student Theses and Dissertations

Several studies have found separation of biogeographic provinces on the West Florida Shelf (WFS), but the location of this separation differs depending on different organisms with faunal boundaries proposed at Apalachicola, Cedar Key, Anclote Key. Tampa Bay, Charlotte Harbor, Cape Romano, or Cape Sable. Biogeographic boundaries can be gradual over a given space and are often species-specific. Analyses of marine benthic mapping and community characterization of Florida’s West-central coast shallow water (depth) hardbottom habitats indicate a major shift in the benthos across Tampa Bay. Quantitative benthic surveys of 29 sites yielded a total of 4,079 individuals of nine stony coral …