Digital Commons Network^™

Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts

Graduate Theses and Dissertations

Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in fibrosis and severe functional deficits which have defied clinical repair strategies. My work spans the design and preclinical evaluation of implants intended to drive the cell community of injured muscle toward a regenerative state, as well as the development of an understanding of the molecular responses of this cell community to biomaterial interventions. I demonstrate a new class of biomaterial by leveraging the productive capacity of sacrificial hollow fiber membrane cell culture; I show specifically that unique threads of whole extracellular matrix can be isolated by solvent degradation …

The Functional Conservation Of Frazzled In Insects, Benjamin Wadsworth

Graduate Theses and Dissertations

Axons in the developing embryo receive and react to signals that direct their growth to reach target tissues at specified locations. The signal pathways that direct midline crossing of axons during embryonic development have been comprehensively examined in the past years using the Drosophila ventral nerve cord or the spinal cord as a model system. A number of these signaling mechanisms are conserved, however disparities have been found between species in general strategy or the molecular signals controlling the response of axons to guidance cues.

The Netrin-Frazzled pathway has been shown to aid in midline crossing of axons in the …

Poultry Labeling: Knowledge, Perceptions, And Preferences Among Adolescents, Eleni L. Parsons

Graduate Theses and Dissertations

The purpose of this study was to determine the knowledge, perceptions, and preferences of adolescents in regard to the labeling of poultry products. The theory of planned behavior (TPB) and Elaboration Likelihood Model (ELM) were used to display the relationship of perceptions, attitude, and behavior intentions. Quantitative data was collected in a descriptive research design. The population included youth, aged 14 – 18 years, involved in the Arkansas 4-H State O’Rama competition (N = 400). The sample (n = 80) ensured a 90% confidence interval of the population. Data was collected by administering paper surveys that addressed four product labels …

The Evolutionary Diversity And Biological Function Of Phenazine Metabolite Biosynthesis In Burkholderia Spp, Samuel Hendry

Master's Theses

Burkholderia encompass a group of ubiquitous Gram-negative bacteria that include numerous saprophytes, as well as several species that cause infections in animals, immunocompromised patients, and plants. Some species of Burkholderia produce colored redox-active secondary metabolites called phenazines (Phz). In the model opportunistic pathogen Pseudomonas aeruginosa, phenazines strongly contribute to the competitiveness, formation of biofilms, and virulence in multiple models of infection. Similar depth of knowledge on the diversity, biosynthesis, and biological functions of phenazines in Burkholderiais missing. This project aimed to bridge this gap in knowledge by focusing on phenazine pathways of B. lata and closely related …

Werewolves: A Three-Dimensional Content Analysis Of Films From 1980-2014, Jennifer Lewis

Master's Theses

WEREWOLVES: A THREE-DIMENSIONAL CONTENT ANALYSIS OF FILMS FROM 1980 – 2014 revolves around how monsters function in stories. Monsters represent fears and teach social norms. They are often portrayed as “other”, but more recently the werewolf has appeared in media as more sympathetic (Brannon 2016, 21; Gilmore 2008, 362; Hughes 2009, 97). Limited research has systematically studied how werewolves are represented in the media. This content analysis focuses on how major werewolf characters are represented in 20 films.

The analysis showcases werewolf characters in today’s culture and what it means to be a monster by analyzing hybridity. This study presents …

Horses, Culture, And Trade: The Impact Of The Horse On Southeastern Native Nations, 1650-1830, Jacob Featherling

Master's Theses

A small portion of the regional literature details the impact of horses on Southeastern Native nations and focuses on a few of the larger groups, particularly the Choctaw, from the mid-eighteenth to the nineteenth century. This thesis intends to increase the scope to analyze the entire Southeastern region, as well as multiple Native nations in the area. The thesis argues that Southeastern Natives slowly adopted horses into their economies and cultures over a longer period of time than previously believed, allowing them to increase their use of horses easily to meet market demands. Instead of southeastern nations rapidly adapting their …

The Effects Of Aqueous Atorvastatin On Steroidogenesis Of Xenopus Laevis At Environmentally Relevant Concentrations, Jeremy Johnson

Master's Theses

Statin drugs are a class of drug that work to reduce endogenous production of cholesterol by competitively inhibiting 3-hydroxy-3-methyl-glutaryl-CoA reductase (Hmgcr) thus inhibiting production of mevalonic acid in the mevalonate pathway. Atorvastatin (Lipitor) is one of the most widely prescribed statin drugs and contamination of wastewater effluent is a growing environmental concern because of the potential to interfere with steroidogenesis in wildlife. Amphibians may be particularly susceptible to the effects of atorvastatin contamination because of their highly permeable integument. I used an amphibian model, Xenopus laevis to test the hypothesis that chronic exposure to low concentrations of atorvastatin …

The Ecology And Evolution Of The Freshwater Turtles Of Southern Mississippi, Gabrielle Berry

Master's Theses

Turtles are one of the most threatened group of animals in existence today. The Southeastern United States is one of two global biodiversity hotspots for turtle species, including the state of Mississippi, where over 30 species can be found. However, very few studies have occurred within the state. This lack of research is even more startling given the ongoing decline, or even extirpation, of numerous turtle species across the world, due to a number of factors, including habitat degradation, and harvest for food or the pet trade.

The overarching goal of this project was to perform a species inclusive freshwater …

Effects Of Light And Macroinvertebrate Consumers On Detrital Microbial Biofilms In Streams, Cheyenne M. Brady

Master's Theses

In lotic freshwater systems, aquatic macroinvertebrates are key processors of biofilms that grow upon organic matter. Although macroinvertebrate effects on biofilms may depend on light availability, the combined effects of consumers and light remain unexplored. Here, I conducted experiments to test effects of presence/absence of the omnivorous shrimp Macrobrachium ohione and the shredding caddisfly Pycnopsyche sp. on Liriodendron tulipifera litter biofilms in experimental streams under light or darkness. I measured litter-associated algal, fungal and bacterial biomasses and production rates, as well as litter decomposition, over 49 days. Both experiments exhibited significant positive effects of light on algal productivity and interactions …

Their Culture Against Them: The Assimilation Of Native American Children Through Progressive Education, 1930-1960s, Jamie Henton

Master's Theses

The failure of the Bureau of Indian Affairs (BIA) to successfully assimilate Native Americans, especially Native children through education tactics such as boarding schools, led to a shift in the mid-twentieth century for pro-Indian reform. From the 1930s through the 1950s, BIA education reformers pursued progressive education. They imagined progressive education would allow the BIA to use Native American traditions and culture to educate and mold Native students into modern contributing American citizens. To appeal to students, the BIA commissioned a series of educational materials, primarily children’s books, designed to use Native culture to teach children how to adapt to …

Vocal Response Of Bottlenose Dolphins (Tursiops Truncatus) To A Novel Stimulus, Lindsey Johnson

Master's Theses

Bottlenose dolphins utilize acoustic signals as their primary mode of communication. Although some aspects of dolphin vocal behavior are well understood, less is known about vocalizations in different behavioral contexts and how these vocal behaviors may indicate habituation and sensitization. The focus of this study was to investigate how bottlenose dolphins respond vocally to a novel stimulus. Archival data from three populations of bottlenose dolphins (N = 20) living in a human-care facility were exposed to a novel apparatus (a mirror) for 10 trials, each lasting 20 minutes. Five of the trials presented the mirror covered with an opaque …

Acute Cardiovascular Response To Low-Load Unilateral, Bilateral, And Alternating Resistance Exercise With Blood Flow Restriction In The Lower Body, Daphney Stanford

Master's Theses

Resistance exercise with blood flow restriction (BFR) has been suggested to exaggerate the exercise pressor response over traditional non-BFR exercise. While applying BFR relative to an individual’s arterial occlusion pressure (AOP) and exercising at low-loads seems to produce a comparable cardiovascular response to traditional moderate or high-load training, it is beneficial to identify modifications for reducing the cardiovascular response to BFR exercise. PURPOSE: To determine if unilateral (UNI), bilateral (BI), or alternating (ALT) exercise modalities elicit different cardiovascular responses during BFR exercise. METHODS: 18 participants (13 male and 5 female) performed four sets of UNI, BI, and ALT knee-extensions at …

Learning Multiple Maps From Conditional Ordinal Triplets, Duy Dung Le, Hady Wirawan Lauw

Research Collection School Of Computing and Information Systems

Ordinal embedding seeks a low-dimensional representation of objects based on relative comparisons of their similarities. This low-dimensional representation lends itself to visualization on a Euclidean map. Classical assumptions admit only one valid aspect of similarity. However, there are increasing scenarios involving ordinal comparisons that inherently reflect multiple aspects of similarity, which would be better represented by multiple maps. We formulate this problem as conditional ordinal embedding, which learns a distinct low-dimensional representation conditioned on each aspect, yet allows collaboration across aspects via a shared representation. Our geometric approach is novel in its use of a shared spherical representation and multiple …

Ltc Newsletter, University Of Dayton. Ryan C. Harris Learning Teaching Center

LTC Newsletter

Fall 2019

Proteomic Changes Across A Natural Temperature Gradient In A Marine Gastropod, M Christina Vasquez, Marilla R. Lippert, Crow White, Ryan K. Walter, Lars Tomanek

Physics

Responses of marine ectotherms to variable environmental temperature often entails maintenance of cellular homeostasis and physiological function through temperature compensation and physiological changes. We investigated the physiological response to thermal stress by examining proteomic changes in the marine kelp forest gastropod and emerging fisheries species Kellet's whelk (Kelletia kelletii) across a naturally-existing thermal gradient that ranges from a warmer-water site inside the species' native range and extends to the northern, cold-water edge of the range. We hypothesized that abundance of cellular stress response and energy metabolism proteins would increase with decreasing temperature in support of cold-compensation. Our exploratory …

Cannabidiol And The Remainder Of The Plant Extract Modulate The Effects Of Δ9-Tetrahydrocannabinol On Fear Memory Reconsolidation, Anthony Murkar, Pam Kent, Christian Cayer, Jon James, Tony Durst, Zul Merali

Brain and Mind Institute

Background: Δ9-Tetrahydrocannabinol (THC, a CB1 receptor agonist) and Cannabidiol (CBD, a non-competitive antagonist of endogenous CB1 and CB2 ligands) are two primary components of Cannabis species, and may modulate fear learning in mammals. The CB1 receptor is widely distributed throughout the cortex and some limbic regions typically associated with fear learning. Humans with posttraumatic disorder (PTSD) have widespread upregulation of CB1 receptor density and reduced availability of endogenous cannabinoid anandamide, suggesting a role for the endocannabinoid system in PTSD. Pharmacological blockade of memory reconsolidation following recall of a conditioned response modulates the expression of learned fear and may represent …

Maladaptation In Feral And Domesticated Animals, Eben Gering, Darren Incorvaia, R. Henriksen, Dominic Wright

Biology Faculty Articles

Selection regimes and population structures can be powerfully changed by domestication and feralization, and these changes can modulate animal fitness in both captive and natural environments. In this review, we synthesize recent studies of these two processes and consider their impacts on organismal and population fitness. Domestication and feralization offer multiple windows into the forms and mechanisms of maladaptation. Firstly, domestic and feral organisms that exhibit suboptimal traits or fitness allow us to identify their underlying causes within tractable research systems. This has facilitated significant progress in our general understandings of genotype–phenotype relationships, fitness trade‐offs, and the roles of population …

Inhibition Of Urothelial Carcinoma By Type I Interferon Activation Of The Innate And Adaptive Immune Response, Devin Plote

Dissertations and Theses (Open Access)

Bacillus Calmette-Guerin (BCG) is the first line therapy for bladder cancer patients with non-invasive disease. However, roughly 40% of patients exhibit no response, tumor recurrence or tumor progression following BCG treatment. Type I interferon (IFN-I) has potent anti-tumor effects against urothelial carcinoma (UC) and may be an alternative treatment option for patients who do not respond to BCG standard of care. However, the mechanisms that mediate the IFN-I-stimulated immune responses against UC are not fully elucidated. Herein, we evaluated the anti-tumor mechanisms of IFN-I in UC by use of adenoviral interferon-α (Ad-IFNα/Syn3) in human patients, and poly(I:C) or lentiviral IFNa …

Games And Roleplaying In The Classroom, Lydia C. Gwyn, Jonathon Wilson

ETSU Faculty Works

The Library Ambassador Program hires approximately 20 undergraduate students each fall and provides them with two semesters of information literacy and research skills instruction before deploying them across campus to help students with their research. As part of their training, instructors use various game-play strategies in the classroom including: a card game designed to teach players about databases and how they function, a card game intended to teach players how to evaluate sources of information, and a roleplaying activity meant to prepare ambassadors for experiences in helping students. Many emotions are involved in the action of gameplay such as competitiveness, …

Space Use And Nesting Ecology Of Common Ravens (Corvus Corax) In Central Washignton State, Brandon A. Rossi

Master of Biology Theses

The ecology and space use of Common ravens (Corvus corax; hereafter ravens) is not well understood in the shrub-steppe of central Washington State. Raven populations have increased by more than 254% since 1990 in Washington State (North American Breeding Bird Survey; www.mbr-pwrc.usgs.gov/bbs/). Ravens in central Washington are implicated as top predators of a small endemic population of greater sage-grouse (Centrocercus urophasianus). Researchers have related increased raven populations to anthropogenic subsidies such as landfills, agriculture, and livestock operations. Analyzing movement can help researchers understand the characteristics of habitat that are important and potentially drive population fluctuations. We found that daily and …

Wildlife Road Mortality Survey Methodologies, Trinity Dale Livingston

Theses and Dissertations

Wildlife mortality due to vehicle collisions is detrimental to species with slower reproduction times, habitat specialists, low density populations, species with large home ranges, or rare species. Vehicle-caused wildlife mortality is an important conservation issue, but before we can ameliorate the effects of road mortalities, we must understand what why it is happening. Wildlife road mortality surveys may be conducted in several ways based on the target species, time available, finances, and safety. Survey methodologies include using accident reports by police and warden agencies, pedestrian road surveys, using citizen scientists, and driving surveys. These methods present a safety risk to …

Toxic Chemical Governance Failure In The United States: Key Lessons And Paths Forward, Ariana M. Chiapella, Zbigniew Grabowski, Mary Ann Rozance, Ashlie D. Denton, Manar A. Alattar, Elise F. Granek

Environmental Science and Management Faculty Publications and Presentations

Over 40 years of regulations in the United States have failed to protect human and environmental health. We contend that these failures result from the flawed governance over the continued production, use, and disposal of toxic chemicals. To address this failure, we need to identify the broader social, political, and technological processes producing, knowing, and regulating toxic chemicals, collectively referred to as toxic chemical governance. To do so, we create a conceptual framework covering five key domains of governance: knowledge production, policy design, monitoring and enforcement, evaluation, and adjudication. Within each domain, social actors of varying power negotiate what constitutes …

Which Credence Production Attributes Do Consumers Prefer? The Case Of Milk, Emmanouil Petrakis

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The interest of consumers for non-traditional food production attributes like antibiotics free and animal welfare have made consumer preferences more complex. These new attributes, usually non-identifiable by inspection or experience of the product are called credence attributes. The objectives of this study are to analyze consumer preferences for milk production attributes by eliciting the relative importance of these attributes for consumers, and to identify possible sources of heterogeneity related to consumer characteristics and actual purchase data. The research method used to elicit consumer preferences is Paired Comparison Method. Consumers from California and Texas were administered the online survey questionnaire in …

Assessing Insect Biodiversity And Promoting Sustainable Practices Toward Pollinators On Campus, Lance A. Durden, Jose A. Sanchez-Ruiz, Debra G. Albanese, Julien M. Buchbinder

Sustainability Showcase (2014-2022)

Dr. Lance Durden, Professor, Department of Biology ($27,560.00)

Added arthropods to the vertebrate biodiversity database that was created in 2015.

Biodiversity Of Pollinators And Predators: Surveying And Increasing Appreciation Of The Bees, Ants And Wasps Of Georgia Southern University, Joshua D. Gibson Phd, Bonnie A. Cobb, Kevin J. Loope Phd

Sustainability Showcase (2014-2022)

Proposed by Drs. Joshua Gibson and Kevin Loope, and Bonnie Cobb (student) Department of Biology. ($28,000.00)

Circulating Serum Fatty Acid Synthase Is Elevated In Patients With Diabetes And Carotid Artery Stenosis And Is Ldl-Associated, Gayan S De Silva, Kshitij Desai, Malik Darwech, Uzma Naim, Xiaohua Jin, Sangeeta Adak, Nikolai Harroun, Luis A Sanchez, Clay F Semenkovich, Mohamed A Zayed

Open Access Publications

BACKGROUND AND AIMS: Diabetes is an independent risk factor for carotid artery stenosis (CAS). Fatty acid synthase (FAS), an essential de novo lipogenesis enzyme, has increased activity in the setting of diabetes that leads to altered lipid metabolism. Circulating FAS (cFAS) was recently observed in the blood of patients with hyperinsulinemia and cancer. We thought to evaluate the origin of cFAS and its role in diabetes-associated CAS.

METHODS: Patients with diabetes and no diabetes, undergoing carotid endarterectomy (CEA) for CAS, were prospectively enrolled for collection of plaque and fasting serum. FPLC was used to purify lipoprotein fractions, and ELISA was …

Novel Cell Adhesion/Migration Pathways Are Predictive Markers Of Hdac Inhibitor Resistance In Cutaneous T Cell Lymphoma, Jared M Andrews, Jennifer A Schmidt, Kenneth R Carson, Amy C Musiek, Neha Mehta-Shah, Jacqueline E Payton

Open Access Publications

BACKGROUND: Treatment for Cutaneous T Cell Lymphoma (CTCL) is generally not curative. Therefore, selecting therapy that is effective and tolerable is critical to clinical decision-making. Histone deacetylase inhibitors (HDACi), epigenetic modifier drugs, are commonly used but effective in only ~30% of patients. There are no predictive markers of HDACi response and the CTCL histone acetylation landscape remains unmapped. We sought to identify pre-treatment molecular markers of resistance in CTCL that progressed on HDACi therapy.

METHODS: Purified T cells from 39 pre/post-treatment peripheral blood samples and skin biopsies from 20 patients were subjected to RNA-seq and ChIP-seq for histone acetylation marks …

Pneumothorax Following Combination Chemotherapy With Bevacizumab: A Case Report And Review Of The Literature, Taha Alrifai, Raya Saba, Dana Rifai, Sarbagya Pandit, Kelley E Kozma

Open Access Publications

Bevacizumab (BV) is a humanized monoclonal antibody that inhibits angiogenesis by targeting vascular endothelial growth factor (VEGF). The addition of BV to combination chemotherapy has been shown to improve the outcomes in several malignancies, including colorectal carcinoma (CRC). However, the use of BV has been associated with adverse effects, including hypertension, hemorrhage, proteinuria, delayed wound healing and bowel perforation. Pneumothorax (PTX) as an adverse event associated with BV use has rarely been reported. We herein report the case of a 68-year-old female patient with a history of metastatic CRC treated with combination chemotherapy, including BV, who presented with complaints of …

Drought Management Before During And After The Drought, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Evaluation Of A Role-Playing Game To Improve Social Skills For Individuals With Asd, Kate A. Helbig

Dissertations

Autism spectrum disorder (ASD) affects one in 59 children (Centers for Disease Control and Prevention, 2018). Impairments in social communication and restricted and repetitive behaviorsare often associated with debilitating outcomes for individuals with ASD. Therefore, it is critical to identify successful treatments to address the social deficits characteristic of ASD. This study investigated the effects of a role-playing game (RPG) on social skill acquisition for individuals with ASD. The primary dependent variable was skill acquisition within the context of the RPG setting. Generalization of skill acquisition outside of the game-context and social functioning was also evaluated. Results indicated that the …