Digital Commons Network^™

Physiological And Genomic Evidence That Selection On The Transcription Factor Epas1 Has Altered Cardiovascular Function In Highaltitude Deer Mice, Rena M. Schweizer, Jonathan P. Velotta, Catherine M. Ivy, Matthew R. Jones, Sarah M. Muir, Gideon S. Bradburd, Jay F. Storz, Graham R. Scott, Zachary A. Cheviron

School of Biological Sciences: Faculty Publications

Evolutionary adaptation to extreme environments often requires coordinated changes in multiple intersecting physiological pathways, but how such multi-trait adaptation occurs remains unresolved. Transcription factors, which regulate the expression of many genes and can simultaneously alter multiple phenotypes, may be common targets of selection if the benefits of induced changes outweigh the costs of negative pleiotropic effects. We combined complimentary population genetic analyses and physiological experiments in North American deer mice (Peromyscus maniculatus) to examine links between genetic variation in transcription factors that coordinate physiological responses to hypoxia (hypoxia-inducible factors, HIFs) and multiple physiological traits that potentially contribute to …

The Prevotella Copri Complex Comprises Four Distinct Clades Underrepresented In Westernized Populations, Adrian Tett, Kun D. Huang, Francesco Asnicar, Hannah Fehlner-Peach, Edoardo Pasolli, Nicolai Karcher, Federica Armanini, Paolo Manghi, Kevin Bonham, Moreno Zolfo, Francesca De Filippis, Cara Magnabosco, Richard Bonneau, John Lusingu, John Amuasi, Karl Reinhard, Thomas Rattei, Fredrik Boulund, Lars Engstrand, Albert Zink, Maria Carmen Collado, Dan R. Littman, Daniel Eibach, Danilo Ercolini, Omar Rota-Stabelli, Curtis Huttenhower, Frank Maixner, Nicola Segata

Karl Reinhard Publications

Prevotella copri is a common human gut microbe that has been both positively and negatively associated with host health. In a cross-continent metaanalysis exploiting >6,500 metagenomes, we obtained >1,000 genomes and explored the genetic and population structure of P. copri. P. copri encompasses four distinct clades (>10% inter-clade genetic divergence) that we propose constitute the P. copri complex, and all clades were confirmed by isolate sequencing. These clades are nearly ubiquitous and co-present in non-Westernized populations. Genomic analysis showed substantial functional diversity in the complex with notable differences in carbohydrate metabolism, suggesting that multi-generational dietary modifications may be …

Imaging Coprolite Taphonomy And Preservation, Karl Reinhard, Morgana Camacho, Breyden Geyer, Samantha Hayek, Chase Horn, Kaitlin Otterson, Julia Russ

Karl Reinhard Publications

The impact of coprolite taphonomy on parasite remains and aDNA recovery has been recognized. In general, coprolites from sites protected by geologic features such as caves and rock shelters exhibit the best preservation. In contrast, coprolites from open sites can be badly affected by taphonomic processes as shown by analyses of parasite eggs. For eggs, the impact of mites and free living nematodes has been quantified. Mites are associated with poor pinworm egg preservation. In other studies, percolation of water through sediments has a negative impact on egg recovery. We note that dietary remains can also decompose at open sites. …

Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein

Department of Entomology: Faculty Publications

The wheat curl mite (WCM), Aceria tosichella Keifer, transmits three potentially devastating viruses to winter wheat. An increased understanding of mite movement and subsequent virus spread through the landscape is necessary to estimate the risk of epidemics by the virus in winter wheat. Owing to the small size of WCMs, their dispersal via wind is hard to monitor; however, the viruses they transmit produce symptoms that can be detected with remote sensing. The objective of this study was to characterize the spatial dispersal of the virus from a central mite-virus source. Virus infection gradients were measured spatially by using aerial …

Batman's Animated Brain(S), Lisa Kort-Butler

Department of Sociology: Presentations

Much of the analysis of Batman’s brain – whether by scholars, writers, or other comic characters – focuses on his psychological make‐up. That is, what makes Bruce Wayne psychologically motivated to be The Batman? His childhood trauma is often poised as the answer, the tireless pursuit of “justice” in an attempt to regain control from the trauma of his parents’ murders (Sanna 2015). The same could be said for his nemeses. Madness, psychopathy, and insanity are centered in the corrupted minds of Gotham’s ghastliest, some of whom have also had psychological or physical traumas (Langley 2012; Lytle 2008). A psychological …

Ecosystem Processes, Land Cover, Climate, And Human Settlement Shape Dynamic Distributions For Golden Eagle Across The Western Us, J.D. Tack, B.R. Noon, Z.H. Bowen, B.C. Fedy

United States Geological Survey: Staff Publications

Species–environment relationships for highly mobile species outside of the breeding season are often highly dynamic in response to the collective effects of everchanging climatic conditions, food resources, and anthropogenic disturbance. Capturing dynamic space-use patterns in a model-based framework is critical as model inference often drives place-based conservation planning. We applied dynamic occupancy models to broad-scale golden eagle Aquila chrysaetos survey data collected annually from 2006 to 2012 during the late summer post-fledging period in the western US. We defined survey sites as 10 km transect segments with a 1 km buffer on either transect side (n = 3540). Derived estimates …

Promoting Change In Common Tern (Sterna Hirundo) Nest Site Selection To Minimize Construction Related Disturbance., Peter C. Mcgowan, Jeffery D. Sullivan, Carl R. Callahan, William Schultz, Jennifer L. Wall, Diann J. Prosser

United States Geological Survey: Staff Publications

Due to the many challenges facing waterbird populations (Jia et al. 2018, Wetlands International 2018), it has become common practice to limit disturbance to breeding colonies whenever possible to maximize reproductive success. While this may require often unpopular management techniques including beach closures (Jorgensen et al. 2015, Mayo et al. 2015) and predator removal (Neuman et al. 2004, Stocking et al. 2017), such actions are sometimes necessary for the success of the colony. However, there are instances when eliminating disturbance is not possible and birds must be attracted to a new site. A common method for attracting waterbirds to a …

Assessment Of Chronic Low-Dose Elemental And Radiological Exposures Of Biota At The Kanab North Uranium Mine Site In The Grand Canyon Watershed, Danielle Cleveland, Jo Ellen Hinck, Julia S. Lankton

United States Geological Survey: Staff Publications

High-grade U ore deposits are in various stages of exploitation across the Grand Canyon watershed, yet the effects of U mining on ecological and cultural resources are largely unknown. Wecharacterized the concentrations of Al, As, Bi, Cd, Co, Cu, Fe, Pb, Hg, Mo, Ni, Se, Ag, Tl, Th, U, and Zn, gross alpha and beta activities, and U and Th radioisotopes in soil, vegetation (Hesperostipa comata, Artemisia tridentata, Tamarix chinensis), and rodents (Peromyscus maniculatus, P. boylii) to waste material at the Kanab North mine, a mine with decades-long surficial contamination, and compared the concentrations (P<0.01) to those at a premining site (Canyon Mine). Rodent tissues were also analyzed for radium-226 and microscopic lesions. Radioactivities and some elemental concentrations (e.g., Co, Pb, U) were greater in the Kanab North mine biological samples than in Canyon Mine biota, indicating a mining-related elemental signature. Mean rodent Ra-226 (111 Bq/kg dry weight [dry wt]) was 3 times greater than expected, indicating radioactive disequilibrium. Multiple soil sample U concentrations exceeded a screening benchmark, growth inhibition thresholds for sensitive plants, and an EC20 for a soil arthropod. Lesions associated with metals exposure were also observed more frequently in rodents at Kanab North than those at Canyon Mine but could not be definitively attributed to U mining. Our results indicate that Kanab North biota have taken up U mining-related elements owing to chronic exposure to surficial contamination. However, no literature-based effects thresholds for small rodents were exceeded, and only a few soil and vegetation thresholds for sensitive species were exceeded; therefore, adverse effects to biota from U mining-related elements at Kanab North are unlikely despite chronic exposure.

Macroeconomic Impact Of Foot‐And‐Mouth Disease Vaccination Strategies For An Outbreak In The Midwestern United States: A Computable General Equilibrium, Maryfrances Miller, Lirong Liu, Steven Shwiff, Stephanie Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

The impacts of alternative responses to a hypothetical foot‐and‐mouth disease (FMD) outbreak occurring in the Midwestern United States are estimated using the Regional Economic Modelling Incorporated Policy Insight + (REMI) computable general equilibrium model, with particular attention paid to the employment impact estimates. The impact on employment and GDP is estimated using forecasts of a 10‐ year period with disease outbreak duration up to 2 years. Fifteen different vaccination protocols are compared to a disease control protocol that relies on animal depopulation with no vaccination. Results show that over the 10‐year study period, the strictly depopulation strategy that made no …

Forage Or Biofuel: Assessing Native Warm-Season Grass Production Among Seed Mixes And Harvest Frequencies Within A Wildlife Conservation Framework, Raymond B. Iglay, Tara J. Conkling, Travis L. Devault, Jerrold L. Belant, James A. Martin

United States Department of Agriculture Wildlife Services: Staff Publications

Native warm-season grasses (NWSG) are gaining merit as biofuel feedstocks for ethanol production with potential for concomitant production of cattle forage and wildlife habitat provision. However, uncertainty continues regarding optimal production approaches for biofuel yield and forage quality within landscapes of competing wildlife conservation objectives. We used a randomized complete block design of 4 treatments to compare vegetation structure, forage and biomass nutrients, and biomass yield between Panicum virgatum (Switchgrass) monocultures and NWSG polycultures harvested once or multiple times near West Point, MS, 2011–2013. Despite taller vegetation and greater biomass in Switchgrass monocultures, NWSG polycultures had greater vegetation structure heterogeneity …

Determination Of Residue Levels Of The Avicide 3-Chloro-4-Methylaniline Hydrochloride In Red-Winged Blackbirds (Agelaius Phoeniceus) By Gas Chromatography-Tandem Mass Spectrometry, David A. Goldade, Kirsten R. Kim, James C. Carlson, Steven F. Volker

United States Department of Agriculture Wildlife Services: Staff Publications

The avicide 3‑chloro‑4‑methylanaline hydrochloride (chloro‑p‑toluidine hydrochloride, CPTH, DRC-1339) is used to control pest bird species that damage agricultural crops. A specific and sensitive gas chromatographytandem mass spectrometry method was developed and validated for the determination of CPTH in avian breast muscle, GI tract, kidney, and liver. Tissue samples were extracted with a solution of acidified water and acetonitrile. The sample was made basic and cleaned up with a combination of liquid-liquid partitioning and solid phase extraction. Separation was achieved using a HP-5 ultra-inert GC column (15 M, 0.25 μm film) with detection on a triple quadrupole mass spectrometer …

Wildlife Management Practices Associated With Pathogen Exposure In Non-Nativewild Pigs In Florida, U.S., Amanda N. Carr, Michael P. Milleson, Felipe A. Hernandez, Hunter R. Merrill, Michael L. Avery, Samantha M. Wisely

United States Department of Agriculture Wildlife Services: Staff Publications

Land use influences disease emergence by changing the ecological dynamics of humans, wildlife, domestic animals, and pathogens. This is a central tenet of One Health, and one that is gaining momentum in wildlife management decision-making in the United States. Using almost 2000 serological samples collected from non-native wild pigs (Sus scrofa) throughout Florida (U.S.), we compared the prevalence and exposure risk of two directly transmitted pathogens, pseudorabies virus (PrV) and Brucella spp., to test the hypothesis that disease emergence would be positively correlated with one of the most basic wildlife management operations: Hunting. The seroprevalence of PrV- …

Necrobiome Framework For Bridging Decomposition Ecology Of Autotrophically And Heterotrophically Derived Organic Matter, M. Eric Benbow, Philip S. Barton, Michael D. Ulyshen, James C. Beasley, Travis L. Devault, Michael S. Strickland, Jeffery K. Tomberlin, Heather R. Jordan, Jennifer L. Pechal

United States Department of Agriculture Wildlife Services: Staff Publications

Decomposition contributes to global ecosystem function by contributing to nutrient recycling, energy flow, and limiting biomass accumulation. The decomposer organisms influencing this process form diverse, complex, and highly dynamic communities that often specialize on different plant or animal resources. Despite performing the same net role, there is a need to conceptually synthesize information on the structure and function of decomposer communities across the spectrum of dead plant and animal resources. A lack of synthesis has limited cross-disciplinary learning and research in important areas of ecosystem and community ecology. Here we expound on the “necrobiome” concept and develop a framework describing …

Non-Native Insects Dominate Daytime Pollination In A High-Elevation Hawaiian Dryland Ecosystem, Clare E. Aslan, Aaron B. Shiels, William Haines, Christina T. Liang

United States Department of Agriculture Wildlife Services: Staff Publications

PREMISE OF THE STUDY: Over one-third of the native flowering plant species in the Hawaiian Islands are listed as federally threatened or endangered. Lack of sufficient pollination could contribute to reductions in populations, reproduction, and genetic diversity among these species but has been little studied.

METHODS: We used systematic observations and manual flower treatments to quantify flower visitation and outcrossing dependency of eight native (including four endangered) plant species in a dryland ecosystem in Hawaii: Argemone glauca, Bidens menziesii, Dubautia linearis, Haplostachys haplostachya, Sida fallax, Silene lanceolata, Stenogyne angustifolia, and Tetramolopium arenarium. …

The Private Law Critique Of International Investment Law, Julian Arato

Articles

This Article argues that investment treaties subtly constrain how nations organize their internal systems of private law, including laws of property, contracts, corporations, and intellectual property. Problematically, the treaties do so on a one-size-fits-all basis, disregarding the wide variation in values reflected in these domestic legal institutions. Investor-state dispute settlement exacerbates this tension, further distorting national private law arrangements. This hidden aspect of the system produces inefficiency, unfairness, and distributional inequities that have eluded the regime's critics and apologists alike.

Social Freedom, Democracy And The Political: Three Reflections On Axel Honneth's Idea Of Socialism, Stephen W. Sawyer, William J. Novak, James T. Sparrow

Articles

Axel Honneth’s Idea of Socialism is an important clarion call for an urgent rethinking of the possibilities of a socialism for the twenty-first century. One of the most surprising and satisfying aspects of Axel Honneth’s timely new book is its recovery of the continued vitality of John Dewey’s pragmatic democratic philosophy. These reflections on Honneth’s use of John Dewey for democratizing social freedom, take stock of and explore the political limits of Honneth’s social reconstruction.

Reparations For Central American Refugees, Sarah R. Sherman-Stokes

Faculty Scholarship

In the midst of vicious and unrelenting attacks on Central American asylum seekers in the United States, this Article seeks to understand historic and present-day patterns of animus and discrimination facing this group of refugees, and to propose solutions. This Article begins by examining decades of prejudice faced by Central American asylum seekers, as well as attempts to right those wrongs through litigation, legislation, and the creation of Temporary Protected Status (TPS). Next, this Article identifies the predominant push and pull factors driving Central American refugees north—and the U.S. role in creating them. The Article then lays out the impact …

When Law Frees Us To Speak, Danielle K. Citron, Jonathon W. Penney

Faculty Scholarship

A central aim of online abuse is to silence victims. That effort is as regrettable as it is successful. In the face of cyber harassment and sexual privacy invasions, women and marginalized groups retreat from online engagement. These documented chilling effects, however, are not inevitable. Beyond its deterrent function, law has an equally important expressive role. In this article, we highlight law’s capacity to shape social norms and behavior through education. We focus on a neglected dimension of law’s expressive role—its capacity to empower victims to express their truths and engage with others. Our argument is theoretical and empirical. We …

Hidden In Plain Sight: A More Compelling Case For Diversity, Jonathan Feingold

Faculty Scholarship

For four decades, the diversity rationale has offered a lifeline to affirmative action in higher education. Yet even after forty years, this critical feature of equal protection doctrine remains constitutionally insecure and politically fraught. Legal challenges persist, the Justice Department has launched a new assault on race-conscious admissions, and an impending shift on the Supreme Court could usher in an era of increased hostility toward the concept of diversity itself. The future of race-conscious admissions arguably hangs in the balance.

In this Article, I argue that the diversity rationale’s present fragility rests, in part, on its defenders’ failure to center …

Attorney As Accompagnateur: Resilient Lawyering When Victory Is Uncertain Or Nearly Impossible, Margaret Reuter, Stephen A. Rosenbaum, Danielle Pelfrey Duryea

Faculty Scholarship

Social justice lawyers come to the profession intending to make a difference through the instruments of law. And gloriously, they often make a difference in people’s lives for the better. They make our world a more just, compassionate, and tolerant place. But there is no denying that, in poverty law practice, legal success can be illusive, ephemeral, or perhaps a mirage. How does that lawyer feel when the legal remedies at her disposal, even if “successful,” fail to mitigate the injustices suffered by her clients? Are there definitions of professional satisfaction and success that are enduring, even if legal success …

The Global Preclinical Antibacterial Pipeline, Ursula Theuretzbacher, Kevin Outterson, Aleks Engel, Anders Karlén

Faculty Scholarship

Antibacterial resistance is a great concern and requires global action. A critical question is whether enough new antibacterial drugs are being discovered and developed. A review of the clinical antibacterial drug pipeline was recently published, but comprehensive information about the global preclinical pipeline is unavailable. This Review focuses on discovery and preclinical development projects and has found, as of 1 May 2019, 407 antibacterial projects from 314 institutions. The focus is on Gram-negative pathogens, particularly bacteria on the WHO priority bacteria list. The preclinical pipeline is characterized by high levels of diversity and interesting scientific concepts, with 135 projects on …

Digitizing Brandenburg: Common Law Drift Toward A Causal Theory Of Imminence, J. Remy Green

Faculty Scholarship

The Supreme Court’s Brandenburg v. Ohio test provides an exception to the First Amendment’s broad guarantee of freedom of speech. Where speech is (1) directed to inciting or producing imminent lawless action, and (2) is likely to incite or produce such action, the First Amendment withdraws its promise of protection. Thus, where the “imminence” of lawless action cannot be shown, free speech cannot be restricted. Since Brandenburg, Courts have applied a test for imminence that turns on proximity in space and in time — that is, the test evaluates how spatiotemporally imminent lawless activity is. In this Article, I argue …

Creation Of An Interdisciplinary Evidence-Based Advocacy Tool: Promoting Medicaid Reimbursement For Pasteurized Donor Milk For Very Low Birth Weight Neonates, Christopher Schmaltz

Yale School of Nursing Digital Theses

An interdisciplinary approach to remove cost barriers associated with full utilization of pasteurized donor human milk in the neonatal intensive care unit. This scholarship produced an evidence-based advocacy tool which allows the user to enter state-specific data to demonstrate the fiscal advantages of implementing pasteurized donor human milk reimbursement policies from a Medicaid payor perspective.

Mta3-Sox2 Module Regulates Cancer Stemness And Contributes To Clinical Outcomes Of Tongue Carcinoma., Zhimeng Yao, Liang Du, Min Xu, Kai Li, Haipeng Guo, Guodong Ye, Dianzheng Zhang, Robert P Coppes, Hao Zhang

PCOM Scholarly Works

Cancer cell plasticity plays critical roles in both tumorigenesis and tumor progression. Metastasis-associated protein 3 (MTA3), a component of the nucleosome remodeling and histone deacetylase (NuRD) complex and multi-effect coregulator, can serve as a tumor suppressor in many cancer types. However, the role of MTA3 in tongue squamous cell cancer (TSCC) remains unclear although it is the most prevalent head and neck cancer and often with poor prognosis. By analyzing both published datasets and clinical specimens, we found that the level of MTA3 was lower in TSCC compared to normal tongue tissues. Data from gene set enrichment analysis (GSEA) also …

Delta-Aminolevulinic Acid-Mediated Photodiagnoses In Surgical Oncology: A Historical Review Of Clinical Trials., Joseph F Georges, Amber Valeri, Huan Wang, Aaron Brooking, Michael Kakareka, Steve S Cho, Zein Al-Atrache, Michael Bamimore, Hany Osman, Joseph Ifrach, Si Yu, Carrie Li, Denah Appelt, John Y K Lee, Peter Nakaji, Kristin Brill, Steven S. Yocom

PCOM Scholarly Works

Fluorescence imaging is an emerging clinical technique for real-time intraoperative visualization of tumors and their boundaries. Though multiple fluorescent contrast agents are available in the basic sciences, few fluorescence agents are available for clinical use. Of the clinical fluorophores, delta aminolevulinic acid (5ALA) is unique for generating visible wavelength tumor-specific fluorescence. In 2017, 5ALA was FDA-approved for glioma surgery in the United States. Additionally, clinical studies suggest this agent may have utility in surgical subspecialties outside of neurosurgery. Data from dermatology, OB/GYN, urology, cardiothoracic surgery, and gastrointestinal surgery show 5ALA is helpful for intraoperative visualization of malignant tissues in multiple …

Reducing Energy Use In Excavators With Biodiesel, Omeed Abolfathi

Construction Management

In assessing the life-cycle cost of a building, the operation energy and construction energy are considered. The operation energy constitutes 90 to 95 percent of the lifetime energy use of a building. Concern over reducing operation energy has led to advancements where many buildings can now be considered net zero. While considerable advancements were made in achieving net zero in the operation, construction energy use has largely remained the same. To achieve net zero for the entire life-cycle of the building, construction energy consumption needs to be reduced. Excavators are one of the most popular pieces of construction equipment. They …

Going (Neo-) Dutch: Establishing Marguerite Gérard’S Artistic Agency, Amanda Strasik

Art Inquiries

No abstract provided.

Delacroix, Joyce Carol Polistena

Art Inquiries

No abstract provided.

Art Inquiries, Xvii:4, Full Issue

Art Inquiries

No abstract provided.

Welcome To Normalton: Leveraging Effective E-Learning Principles For Adult Learners, Robert L. Moore

STEMPS Faculty Publications

This design case details the critical design decisions used in the development of an e-learning module library for North Carolina local government officials focused on land use regulations. These modules cover topics from an introduction to land use regulations, to evidentiary hearing conduct guidelines, defining vested rights, and explaining how to adopt and amend an ordinance. This project was in response to the North Carolina League of Municipalities (NCLM) members’ increased requests for training in this subject area. This organization requested the assistance of the two faculty members at the University of North Carolina at Chapel Hill School of Government …