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2019

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Mycoprotein: The Future Of Nutritious Nonmeat Protein, A Symposium Review, Tim Ja Finnigan, Benjamin T. Wall, Peter J. Wilde, Francis B. Stephens, Steve Taylor, Marjorie R. Freedman Jan 2019

Mycoprotein: The Future Of Nutritious Nonmeat Protein, A Symposium Review, Tim Ja Finnigan, Benjamin T. Wall, Peter J. Wilde, Francis B. Stephens, Steve Taylor, Marjorie R. Freedman

Department of Food Science and Technology: Faculty Publications

Mycoprotein is an alternative, nutritious protein source with a meat-like texture made from Fusarium venenatum, a naturally occurring fungus. Its unique method of production yields a significantly reduced carbon and water footprint relative to beef and chicken. Mycoprotein, sold as Quorn, is consumed in 17 countries, including the United States. In line with current dietary guidelines, mycoprotein is high in protein and fiber, and low in fat, cholesterol, sodium, and sugar. Mycoprotein may help maintain healthy blood cholesterol levels, promote muscle synthesis, control glucose and insulin levels, and increase satiety. It is possible that some susceptible consumers will become sensitized, …


Extraction Of Astaxanthin From Engineered Camelina Sativa Seed Using Ethanol-Modified Supercritical Carbon Dioxide, Liyang Xie, Edgar B. Cahoon, Yue Zhang, Ozan Ciftci Jan 2019

Extraction Of Astaxanthin From Engineered Camelina Sativa Seed Using Ethanol-Modified Supercritical Carbon Dioxide, Liyang Xie, Edgar B. Cahoon, Yue Zhang, Ozan Ciftci

Department of Food Science and Technology: Faculty Publications

Natural astaxanthin, a high-value carotenoid that is currently extracted mainly from marine organisms, was extracted from engineered camelina seed using ethanol-modified supercritical carbon dioxide (SC-CO2) for the first time, and compared with hexane and accelerated solvent extraction using hexane and ethanol. Response surface methodology (RSM) based on central composite rotatable design was employed to investigate the effect of pressure (30–45 MPa), temperature (40–60 °C), and ethanol concentration (10–35%, w/w). RSM-optimized conditions (41.6 MPa, 36.6 °C and 42.0% ethanol concentration) predicted the astaxanthin concentration as 437 μg/g oil, whereas the actual concentration was 421 ± 14 μg/g oil. Astaxanthin …


The Microbiomes Of Pancreatic And Duodenum Tissue Overlap And Are Highly Subject Specific But Differ Between Pancreatic Cancer And Non-Cancer Subjects, Erika Del Castillo, Richard Meier, Mei Chung, Devin C. Koestler, Tsute Chen, Bruce J. Paster, Kevin P. Charpentier, Karl T. Kelsey, Jacques Izard, Dominique S. Michaud Jan 2019

The Microbiomes Of Pancreatic And Duodenum Tissue Overlap And Are Highly Subject Specific But Differ Between Pancreatic Cancer And Non-Cancer Subjects, Erika Del Castillo, Richard Meier, Mei Chung, Devin C. Koestler, Tsute Chen, Bruce J. Paster, Kevin P. Charpentier, Karl T. Kelsey, Jacques Izard, Dominique S. Michaud

Department of Food Science and Technology: Faculty Publications

Background: In mice, bacteria from the mouth can translocate to the pancreas and impact pancreatic cancer progression. In humans, oral bacteria associated with periodontal disease have been linked to pancreatic cancer risk. It is not known if DNA bacterial profiles in the pancreas and duodenum are similar within individuals.

Methods: Tissue samples were obtained from 50 subjects with pancreatic cancer or other conditions requiring foregut surgery at the Rhode Island Hospital (RIH), and from thirty-four organs obtained from the National Disease Research Interchange. 16S rRNA gene sequencing was performed on 189 tissue samples (pancreatic duct, duodenum, pancreas), 57 …


Effects Of A Comprehensive Nutrition Education Programme To Change Grade 4 Primary-School Students’ Eating Behaviours In China, Lian Qian, Ian M. Newman, Lok-Wa Yuen, Weijing Du, Duane F. Shell Jan 2019

Effects Of A Comprehensive Nutrition Education Programme To Change Grade 4 Primary-School Students’ Eating Behaviours In China, Lian Qian, Ian M. Newman, Lok-Wa Yuen, Weijing Du, Duane F. Shell

Department of Educational Psychology: Faculty Publications

Objective: As part of a national initiative to reduce child obesity, a comprehensive school-based nutrition education intervention to change eating behaviours among grade 4 primary-school students was developed, implemented and evaluated. Design: The intervention was developed by school staff, with technical assistance from outside health education specialists. The programme included school facility upgrades, school teacher/staff training, curriculum changes and activities for parents. Student scores on nine key eating behaviours were assessed prior to and after the programme. The quality of programme implementation in the schools was monitored by technical assistance teams. Setting: Shandong Province (high household income) and Qinghai Province …


Five-Minute Cognitive Test As A New Quick Screening Of Cognitive Impairment In The Elderly, Jie Zhang, Lijun Wang, Xia Deng, Guoqiang Fei, Lirong Jin, Xiaoli Pan, Liuhan Cai, Anthony D. Albano, Chunjiu Zhong Jan 2019

Five-Minute Cognitive Test As A New Quick Screening Of Cognitive Impairment In The Elderly, Jie Zhang, Lijun Wang, Xia Deng, Guoqiang Fei, Lirong Jin, Xiaoli Pan, Liuhan Cai, Anthony D. Albano, Chunjiu Zhong

Department of Educational Psychology: Faculty Publications

This study aims to develop a new evaluation method for quickly and conveniently screening cognitive impairment in the elderly. The five-minute cognitive test (FCT) was designed to capture deficits in five domains of cognitive abilities, including episodic memory, language fluency, time orientation, visuospatial function, and executive function. Subsequently, FCT efficiencies in differentiating normally cognitive ability from cognitive impairment were explored and compared with that of the Mini-Mental Status Evaluation (MMSE). Equipercentile equating method was utilized to create a crosswalk between scores of the FCT and MMSE. Further, the association of scores of the FCT and MMSE with hippocampal volumes was …


How People Make Sense Of Drones Used For Atmospheric Science (And Other Purposes): Hopes, Concerns, And Recommendations, Janell C. Walther, Lisa M. Pytlikzillig, Carrick Detweiler, Adam L. Houston Jan 2019

How People Make Sense Of Drones Used For Atmospheric Science (And Other Purposes): Hopes, Concerns, And Recommendations, Janell C. Walther, Lisa M. Pytlikzillig, Carrick Detweiler, Adam L. Houston

Lisa PytlikZillig Publications

Unmanned aerial systems (UAS) can advance understanding of the atmosphere and improve weather prediction, but public perceptions of drone technologies need to be assessed to ensure successful societal integration. Our qualitative study examines public perceptions of UAS technology, and the associated risks and benefits, for such civilian purposes. We examine how people form perceptions, and discuss the implications of these perceptions for UAS design and regulation. Our study finds the public to be favorable toward UAS used for “noble” purposes. Participant views are informed by popular media, personal experiences, comparisons between technologies, and consideration of the trustworthiness of the users, …


Perceptions Of Library And Information Science Professionals Towards Internet Of Things (Iot), Anna Kaushik Dr. Jan 2019

Perceptions Of Library And Information Science Professionals Towards Internet Of Things (Iot), Anna Kaushik Dr.

Library Philosophy and Practice (e-journal)

In the present era, the Internet of Things (IoT) is considering a hot topic in all disciplines and spreading rapidly in different domains including library and information science sphere. Thus, the library and information science professionals are fascinating greatly towards the Internet of Things (IoT). This study aims to get perceptions of library and information science professional towards the Internet of Things (IoT). The results of the study revealed that most of library and information science professionals who participated in this survey belonged to academic libraries, working on librarian's designation, known (IoT) concept from online sources and from two years. …


Spatiotemporal Characterizations Of Spontaneously Beating Cardiomyocytes With Adaptive Reference Digital Image Correlation, Akankshya Shradhanjali, Brandon D. Riehl, Bin Duan, Ruiguo Yang, Jung Yul Lim Jan 2019

Spatiotemporal Characterizations Of Spontaneously Beating Cardiomyocytes With Adaptive Reference Digital Image Correlation, Akankshya Shradhanjali, Brandon D. Riehl, Bin Duan, Ruiguo Yang, Jung Yul Lim

Department of Mechanical and Materials Engineering: Faculty Publications

We developed an Adaptive Reference-Digital Image Correlation (AR-DIC) method that enables unbiased and accurate mechanics measurements of moving biological tissue samples. We applied the AR-DIC analysis to a spontaneously beating cardiomyocyte (CM) tissue, and could provide correct quantifications of tissue displacement and strain for the beating CMs utilizing physiologically-relevant, sarcomere displacement length-based contraction criteria. The data were further synthesized into novel spatiotemporal parameters of CM contraction to account for the CM beating homogeneity, synchronicity, and propagation as holistic measures of functional myocardial tissue development. Our AR-DIC analyses may thus provide advanced non-invasive characterization tools for assessing the development of spontaneously …


Mechanical Stresses Associated With Flattening Of Human Femoropopliteal Artery Specimens During Planar Biaxial Testing And Their Effects On The Calculated Physiologic Stress-Stretch State, Majid Jadidi, Anastasia Desyatova, Jason N. Mactaggart, Alexey Kamenskiy Jan 2019

Mechanical Stresses Associated With Flattening Of Human Femoropopliteal Artery Specimens During Planar Biaxial Testing And Their Effects On The Calculated Physiologic Stress-Stretch State, Majid Jadidi, Anastasia Desyatova, Jason N. Mactaggart, Alexey Kamenskiy

Department of Mechanical and Materials Engineering: Faculty Publications

Planar biaxial testing is commonly used to characterize the mechanical properties of arteries, but stresses associated with specimen flattening during this test are unknown. We quantified flattening effects in human femoropopliteal arteries (FPAs) of different ages, and determined how they affect the calculated arterial physiologic stress-stretch state. Human FPAs from 472 tissue donors (age 12-82 years, mean 53±16 years) were tested using planar biaxial extension, and morphometric and mechanical characteristics were used to assess the flattening effects. Constitutive parameters for the invariant-based model were adjusted to account for specimen flattening, and used to calculate the physiologic stresses, stretches, axial force, …


Diurnal Habitat Selection Of Migrating Whooping Crane In The Great Plains, David M. Baasch, Patrick D. Farrell, Aaron T. Pearse, David A. Brandt, Andrew J. Caven, Mary J. Harner, Greg D. Wright, Kristine L. Metzger Jan 2019

Diurnal Habitat Selection Of Migrating Whooping Crane In The Great Plains, David M. Baasch, Patrick D. Farrell, Aaron T. Pearse, David A. Brandt, Andrew J. Caven, Mary J. Harner, Greg D. Wright, Kristine L. Metzger

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Available stopover habitats with quality foraging opportunities are essential for migrating waterbirds, including Whooping Crane (Grus americana). Several studies have evaluated habitats used by Whooping Crane for roosting throughout its migration corridor; however, habitats associated with foraging and other diurnal activities have received less attention. We used data collected from 42 Whooping Crane individuals that included 2169 diurnal use locations within 395 stopover sites evaluated during spring 2013 to fall 2015 to assess diurnal habitat selection throughout the U.S. portion of the migration corridor. We found that Whooping Crane selected wetland land-cover types (i.e., open water, riverine, and semipermanent wetlands) …


U.S. Geological Survey- Northern Prairie Wildlife Research Center 2017 Research Activity Report, Mark H. Sherfy Jan 2019

U.S. Geological Survey- Northern Prairie Wildlife Research Center 2017 Research Activity Report, Mark H. Sherfy

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Contents

Acknowledgments...............................................................................iii

Center Mission and Science Strategy...............................................................1

Lines of Work..................................................................................2

Study Narratives.................................................................................16


Mortality In Aransas-Wood Buffalo Whooping Cranes: Timing, Location, And Causes, Aaron T. Pearse, David A. Brandt, Barry K. Hartup, Mark T. Bidwell Jan 2019

Mortality In Aransas-Wood Buffalo Whooping Cranes: Timing, Location, And Causes, Aaron T. Pearse, David A. Brandt, Barry K. Hartup, Mark T. Bidwell

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The Aransas-Wood Buffalo Population (AWBP) of Whooping Cranes (Grus americana) has experienced a population growth rate of approximately 4% for multiple decades (Butler et al., 2014a; Miller et al., 1974). Population growth for long-lived species of birds is generally highly sensitive to variation in adult mortality rates (Sæther and Bakke, 2000). A population model for endangered Red-crowned Cranes (Grus japonensis) in Japan conforms to this pattern, where growth rate is most sensitive to adult mortality (Masatomi et al., 2007). Earlier analyses observed that the AWBP growth rate increased in the mid-1950s and that this increase was likely caused by reduced …


Persistent Viral Reservoirs In Lymphoid Tissues In Siv-Infected Rhesus Macaques Of Chinese-Origin On Suppressive Antiretroviral Therapy, Summer Siddiqui, Stefanie Perez, Yong Gao, Lara Doyle-Meyers, Brian T. Foley, Qingsheng Li, Binhua Ling Jan 2019

Persistent Viral Reservoirs In Lymphoid Tissues In Siv-Infected Rhesus Macaques Of Chinese-Origin On Suppressive Antiretroviral Therapy, Summer Siddiqui, Stefanie Perez, Yong Gao, Lara Doyle-Meyers, Brian T. Foley, Qingsheng Li, Binhua Ling

Nebraska Center for Virology: Faculty Publications

Understanding HIV latent reservoirs in tissues is essential for the development of new strategies targeting these sites for eradication. Here, we assessed the size of latent reservoirs and the source of residual viruses in multiple lymphoid tissues of SIV-infected and fully suppressed rhesus macaques of Chinese-origin (cRMs). Eight cRMs were infected with SIVmac251 and treated with tenofovir and emtricitabine daily for 24 weeks initiated 4 weeks post-infection. Four of the eight animals reached sustained full viral suppression with undetectable viremia. The levels of cell-associated SIV DNA varied in peripheral blood mononuclear cells (PBMCs) and multiple lymphoid tissues, but with higher …


Near-Atomic Structure Of A Giant Virus, Qianglin Fang, Dongjie Zhu, Irina Agarkova, Jagat Adhikari, Thomas Klose, Yue Liu, Zhenguo Chen, Yingyuan Sun, Michael L. Gross, James L. Van Etten, Xinzheng Zhang, Michael G. Rossmann Jan 2019

Near-Atomic Structure Of A Giant Virus, Qianglin Fang, Dongjie Zhu, Irina Agarkova, Jagat Adhikari, Thomas Klose, Yue Liu, Zhenguo Chen, Yingyuan Sun, Michael L. Gross, James L. Van Etten, Xinzheng Zhang, Michael G. Rossmann

Nebraska Center for Virology: Faculty Publications

Although the nucleocytoplasmic large DNA viruses (NCLDVs) are one of the largest group of viruses that infect many eukaryotic hosts, the near-atomic resolution structures of these viruses have remained unknown. Here we describe a 3.5 Å resolution icosahedrally averaged capsid structure of Paramecium bursaria chlorella virus 1 (PBCV-1). This structure consists of 5040 copies of the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which there are 13 different types. The minor capsid proteins form a hexagonal network below the outer capsid shell, stabilizing the capsid by binding neighboring capsomers together. The size …


A Single-Cycle Adenovirus Type 7 Vaccine For Prevention Of Acute Respiratory Disease, Brianna L. Bullard, Brigette N. Corder, Eric A. Weaver Jan 2019

A Single-Cycle Adenovirus Type 7 Vaccine For Prevention Of Acute Respiratory Disease, Brianna L. Bullard, Brigette N. Corder, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Adenovirus type 7 (Ad7) infection is associated with acute respiratory disease (ARD), especially in military recruits living in close quarters. Recently, several outbreaks of Ad7 infections have occurred in civilian populations, with some cases leading to death. However, the current Ad7 vaccine is licensed for use only in military recruits because it utilizes an orally delivered wild type virus which is shed in the stool for 28 days after immunization. This poses a safety risk due to the possibility of virus spread to vulnerable populations. To address the need for a safer Ad7 vaccine for use in civilian populations, we …


A Double Humanized Blt-Mice Model Featuring A Stable Human-Like Gut Microbiome And Human Immune System, Lance Daharsh, Jianshui Zhang, Amanda E. Ramer-Tait, Qingsheng Li Jan 2019

A Double Humanized Blt-Mice Model Featuring A Stable Human-Like Gut Microbiome And Human Immune System, Lance Daharsh, Jianshui Zhang, Amanda E. Ramer-Tait, Qingsheng Li

Nebraska Center for Virology: Faculty Publications

Humanized mice (hu-mice) that feature a functional human immune system have fundamentally changed the study of human pathogens and disease. They can be used to model diseases that are otherwise difficult or impossible to study in humans or other animal models. The gut microbiome can have a profound impact on human health and disease. However, the murine gut microbiome is very different than the one found in humans. There is a need for improved pre-clinical hu-mice models that have an engrafted human gut microbiome. Therefore, we created double hu-mice that feature both a human immune system and stable human-like gut …


Born To Take Risk? The Effect Of Ceo Birth Order On Strategic Risk Taking, Robert J. Campbell, Seung-Hwan Jeong, Scott D. Graffin Jan 2019

Born To Take Risk? The Effect Of Ceo Birth Order On Strategic Risk Taking, Robert J. Campbell, Seung-Hwan Jeong, Scott D. Graffin

Department of Management: Faculty Publications

The importance of birth order has been the subject of debate for centuries, and has captured the attention of the general public and researchers alike. Despite this interest, scholars have little understanding of the impact birth order has on CEOs and their strategic decisions. With this in mind, we develop theory that explains how CEO birth order may be associated with strategic risk taking. Drawing from evolutionary theory arguments related to birth order,we theorize that CEO birth order is positively associatedwith strategic risk taking; that is, earlier-born CEOswill take less risk than later-born CEOs. As evolutionary theory proposes that birth …


A Small Viral Potassium Ion Channel With An Inherent Inward Rectification, Denise Eckert, Tobias Schulze, Julian Stahl, Oliver Rauh, James L. Van Etten, Brigitte Hertel, Indra Schroeder, Anna Moroni, Gerhard Thiel Jan 2019

A Small Viral Potassium Ion Channel With An Inherent Inward Rectification, Denise Eckert, Tobias Schulze, Julian Stahl, Oliver Rauh, James L. Van Etten, Brigitte Hertel, Indra Schroeder, Anna Moroni, Gerhard Thiel

Department of Plant Pathology: Faculty Publications

Some algal viruses have coding sequences for proteins with structural and functional characteristics of pore modules of complex K+ channels. Here we exploit the structural diversity among these channel orthologs to discover new basic principles of structure/function correlates in K+ channels. The analysis of three similar K+ channels with ≤ 86 amino acids (AA) shows that one channel (Kmpv1) generates an ohmic conductance in HEK293 cells while the other two (KmpvSP1, KmpvPL1) exhibit typical features of canonical Kir channels. Like Kir channels, the rectification of the viral channels is a function …


Cryopreservation Of Paramecium Bursaria Chlorella Virus-1 During An Active Infection Cycle Of Its Host, Samantha R. Coy, Alyssa N. Alsante, James L. Van Etten, Steven W. Wilhelm Jan 2019

Cryopreservation Of Paramecium Bursaria Chlorella Virus-1 During An Active Infection Cycle Of Its Host, Samantha R. Coy, Alyssa N. Alsante, James L. Van Etten, Steven W. Wilhelm

Department of Plant Pathology: Faculty Publications

Best practices in laboratory culture management often include cryopreservation of microbiota, but this can be challenging with some virus particles. By preserving viral isolates researchers can mitigate genetic drift and laboratory-induced selection, thereby maintaining genetically consistent strains between experiments. To this end, we developed a method to cryopreserve the model, green-alga infecting virus, Paramecium bursaria Chlorella virus 1 (PBCV-1). We explored cryotolerance of the infectivity of this virus particle, whereby freezing without cryoprotectants was found to maintain the highest infectivity (~2.5%). We then assessed the cryopreservation potential of PBCV-1 during an active infection cycle in its Chlorella variabilis NC64A host, …


Pancreas Of Coxsackievirus-Infected Dams And Their Challenged Pups: A Complex Issue, Sona Sarmirova, Maria Borsanyiova, Brigita Benkoova, Michaela Pospisilova, Rajkumar Rajasekaran, Katarina Berakova, Pavol Gomolcak, Jay Reddy, Shubhada Bopegamage Jan 2019

Pancreas Of Coxsackievirus-Infected Dams And Their Challenged Pups: A Complex Issue, Sona Sarmirova, Maria Borsanyiova, Brigita Benkoova, Michaela Pospisilova, Rajkumar Rajasekaran, Katarina Berakova, Pavol Gomolcak, Jay Reddy, Shubhada Bopegamage

School of Veterinary and Biomedical Sciences: Faculty Publications

Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology …


The Role Of Isothiocyanates As Cancer Chemo‐Preventive, Chemo‐Therapeutic And Anti‐Melanoma Agents, Melina Mitsiogianni, Georgios Koutsidis, Nikos Mavroudis, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Vasilis Zoumpourlis, Tom Amery, Alex Galanis, Aglaia Pappa, Mihalis I. Panayiotidis Jan 2019

The Role Of Isothiocyanates As Cancer Chemo‐Preventive, Chemo‐Therapeutic And Anti‐Melanoma Agents, Melina Mitsiogianni, Georgios Koutsidis, Nikos Mavroudis, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Vasilis Zoumpourlis, Tom Amery, Alex Galanis, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Many studies have shown evidence in support of the beneficial effects of phytochemicals in preventing chronic diseases, including cancer. Among such phytochemicals, sulphur‐containing compounds (e.g., isothiocyanates (ITCs)) have raised scientific interest by exerting unique chemopreventive properties against cancer pathogenesis. ITCs are the major biologically active compounds capable of mediating the anticancer effect of cruciferous vegetables. Recently, many studies have shown that a higher intake of cruciferous vegetables is associated with reduced risk of developing various forms of cancers primarily due to a plurality of effects, including (i) metabolic activation and detoxification, (ii) inflammation, (iii) angiogenesis, (iv) metastasis and (v) regulation …


Viral Emergence In Marine Mammals In The North Pacific May Be Linked To Arctic Sea Ice Reduction, Elizabeth Vanwormer, J. A.K. Mazet, A. Hall, V. A. Gill, P. L. Boveng, J. M. London, T. Gelatt, B. S. Fadely, M. E. Lander, J. Sterling, V. N. Burkanov, R. R. Ream, P. M. Brock, L. D. Rea, B. R. Smith, A. Jeffers, M. Henstock, M. J. Rehberg, K. A. Burek-Huntington, S. L. Crosby, J. A, Hammond, T. Goldstein Jan 2019

Viral Emergence In Marine Mammals In The North Pacific May Be Linked To Arctic Sea Ice Reduction, Elizabeth Vanwormer, J. A.K. Mazet, A. Hall, V. A. Gill, P. L. Boveng, J. M. London, T. Gelatt, B. S. Fadely, M. E. Lander, J. Sterling, V. N. Burkanov, R. R. Ream, P. M. Brock, L. D. Rea, B. R. Smith, A. Jeffers, M. Henstock, M. J. Rehberg, K. A. Burek-Huntington, S. L. Crosby, J. A, Hammond, T. Goldstein

School of Veterinary and Biomedical Sciences: Faculty Publications

Climate change-driven alterations in Arctic environments can influence habitat availability, species distributions and interactions, and the breeding, foraging, and health of marine mammals. Phocine distemper virus (PDV), which has caused extensive mortality in Atlantic seals, was confirmed in sea otters in the North Pacific Ocean in 2004, raising the question of whether reductions in sea ice could increase contact between Arctic and sub-Arctic marine mammals and lead to viral transmission across the Arctic Ocean. Using data on PDV exposure and infection and animal movement in sympatric seal, sea lion, and sea otter species sampled in the North Pacific Ocean from …


Exploring A New Determinant Of Task Technology Fit: Content Characteristics, Chongwoo Park Jan 2019

Exploring A New Determinant Of Task Technology Fit: Content Characteristics, Chongwoo Park

Journal of International Technology and Information Management

Although task technology fit has received academic attention as a measure of information systems success, compared to other popular information systems success models it has received less empirical testing in the information systems field. One reason might be a relatively low explanatory power for the main construct, i.e., task technology fit, in the model. In this study, we suggest content characteristics as a new determinant of fit, and empirically test it along with the two existing determinants, task and technology characteristics with 105 users of a web-based learning management system. In order to verify the contribution of content characteristics to …


Use My Digital Forensics Tool...It’S Shiny!, Kara Nance, Vincent Nestler, Matt Bishop Jan 2019

Use My Digital Forensics Tool...It’S Shiny!, Kara Nance, Vincent Nestler, Matt Bishop

Journal of International Technology and Information Management

The tendency to use technologies without fully understanding the potential ramifications extends to all reaches of our lives. Digital forensics is not immune from this phenomenon. This paper discusses some past scenarios in which conclusions were drawn before all of the testing was complete. Digital forensics tools are then discussed including tool capabilities, tool analysis, and associated challenges. It identifies some potential issues and ramifications that may not be given appropriate consideration by digital forensic examiners or those who rely on these tools when weighing evidence. It concludes with some suggestions for future research directions that could answer some important …


Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian Jan 2019

Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background: Cartilage repair outcomes are compromised in a pro-inflammatory environment; therefore, the mitigation of pro-inflammatory responses is beneficial. Treatment with continuous low-intensity ultrasound (cLIUS) at the resonant frequency of 5 MHz is proposed for the repair of chondral fissures under pro-inflammatory conditions.

Methods: Bovine osteochondral explants, concentrically incised to create chondral fissures, were maintained under cLIUS (14 kPa (5 MHz, 2.5 Vpp), 20 min, 4 times/day) for a period of 28 days in the presence or absence of cytokines, interleukin-6 (IL-6) or tumor necrosis factor (TNF)α. Outcome assessments included histological and immunohistochemical staining of the explants; and the expression of …


Alcohol Metabolism Potentiates Hiv-Induced Hepatotoxicity: Contribution To End-Stage Liver Disease, Murali Ganesan, Moses New-Aaron, Raghubendra Singh Dagur, Edward Makarov, Weimin Wang, Kusum K. Kharbanda, Srivatsan Kidambi, Larisa Y. Poluektova, Natalia A. Osna Jan 2019

Alcohol Metabolism Potentiates Hiv-Induced Hepatotoxicity: Contribution To End-Stage Liver Disease, Murali Ganesan, Moses New-Aaron, Raghubendra Singh Dagur, Edward Makarov, Weimin Wang, Kusum K. Kharbanda, Srivatsan Kidambi, Larisa Y. Poluektova, Natalia A. Osna

Department of Chemical and Biomolecular Engineering: Faculty Publications

In an era of improved survival due to modern antiretroviral therapy, liver disease has become a major cause of morbidity and mortality, resulting in death in 15–17% of human immunodeficiency virus (HIV)-infected patients. Alcohol enhances HIV-mediated liver damage and promotes the progression to advanced fibrosis and cirrhosis. However, the mechanisms behind these events are uncertain. Here, we hypothesize that ethanol metabolism potentiates accumulation of HIV in hepatocytes, causing oxidative stress and intensive apoptotic cell death. Engulfment of HIV-containing apoptotic hepatocytes by non-parenchymal cells (NPCs) triggers their activation and liver injury progression. This study was performed on primary human hepatocytes and …


Fire Legacies In Eastern Ponderosa Pine Forests, Caleb P. Roberts, Victoria M. Donovan, Carissa L. Wonkka, Larkin A. Powell, Craig R. Allen, David G. Angeler, David A. Wedin, Dirac L. Twidwell Jr Jan 2019

Fire Legacies In Eastern Ponderosa Pine Forests, Caleb P. Roberts, Victoria M. Donovan, Carissa L. Wonkka, Larkin A. Powell, Craig R. Allen, David G. Angeler, David A. Wedin, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Disturbance legacies structure communities and ecological memory, but due to increasing changes in disturbance regimes, it is becoming more difficult to characterize disturbance legacies or determine how long they persist. We sought to quantify the characteristics and persistence of material legacies (e.g., biotic residuals of disturbance) that arise from variation in fire severity in an eastern ponderosa pine forest in North America. We compared forest stand structure and understory woody plant and bird community composition and species richness across unburned, low‐, moderate‐, and high‐severity burn patches in a 27‐year‐old mixed‐severity wildfire that had received minimal post‐fire management. We identified distinct …


Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joseph Chappell Jan 2019

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joseph Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …


Spatial Variability Of Soil Properties Under Different Land Use In The Dang District Of Nepal, Dinesh Panday, Roshan Babu Ojha, Devraj Chalise, Saurav Das, Bikesh Twanabasu Jan 2019

Spatial Variability Of Soil Properties Under Different Land Use In The Dang District Of Nepal, Dinesh Panday, Roshan Babu Ojha, Devraj Chalise, Saurav Das, Bikesh Twanabasu

Department of Agronomy and Horticulture: Faculty Publications

Increased nutrient mining, soil erosion, and limited nutrient management has led to declines in soil quality and reduced productivity in many parts of Nepal. A study was conducted in the eastern part of the Dang district of Nepal in 2015 to assess the variability of selected soil properties of three different land use types (agricultural, agroforestry, and grassland) and to map their spatial distribution. A total of 120 soil samples were collected from 0–15 cm depth and analyzed for soil fertility parameters: pH, organic matter (OM), nitrogen (N), phosphorus (P), potassium (K), boron (B), and zinc (Zn). Results revealed that …


Cover Crop Mixture Diversity, Biomass Productivity, Weed Suppression, And Stability, A. M. Florence, Leon G. Higley, R. A. Drijber, Charles A. Francis, John L. Lindquist Jan 2019

Cover Crop Mixture Diversity, Biomass Productivity, Weed Suppression, And Stability, A. M. Florence, Leon G. Higley, R. A. Drijber, Charles A. Francis, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

The diversity-productivity, diversity-invasibility, and diversity-stability hypotheses propose that increasing species diversity should lead, respectively, to increased average biomass productivity, invasion resistance, and stability. We tested these three hypotheses in the context of cover crop mixtures, evaluating the effects of increasing cover crop mixture diversity on above ground biomass, weed suppression, and biomass stability. Twenty to forty cover crop treatments were replicated three or four times at eleven sites using eighteen species representing three cover crop species each from six pre-defined functional groups: cool-season grasses, cool-season legumes, cool-season brassicas, warm-season grasses, warm-season legumes, and warm-season broadleaves. Each species was seeded as …