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Polymeric Nanocarriers Functionalized With Peptides For Improved Glioblastoma Targeting And Blood–Brain Barrier Permeability, Cristian Antonio Wieczorek Villas Boas, Aaron Priester, Buck E. Rogers, Anthony J. Convertine Aug 2025

Polymeric Nanocarriers Functionalized With Peptides For Improved Glioblastoma Targeting And Blood–Brain Barrier Permeability, Cristian Antonio Wieczorek Villas Boas, Aaron Priester, Buck E. Rogers, Anthony J. Convertine

Materials Science and Engineering Faculty Research & Creative Works

We report the synthesis of polymeric conjugates designed to penetrate the blood–brain barrier (BBB) and selectively bind glioblastoma (GBM) cells through reversible addition–fragmentation chain transfer (RAFT) polymerization. The resulting materials were engineered to contain peptide macromonomers for cell-specific targeting and integrated DOTA units to facilitate radiolabeling with copper-64 (64Cu), yielding radiolabeled conjugates with greater than 95% radiochemical purity. In biodistribution assessments conducted in mice, C1C2 peptide-conjugated polymers showed significantly improved accumulation in brain tissue, supported by brain perfusion analyses confirming efficient BBB penetration. Additionally, flow cytometry evaluations demonstrated specific affinity of GBM-targeted polymer formulations toward U87 glioblastoma cells. …

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Targeted Proteomic Analysis Identifies Acvrl1 As A Marker Of Cerebral Edema In Aneurysmal Subarachnoid Hemorrhage, Bosco Seong Kyu Yang, Lena O'Keefe, Sarah Hinds, Hua Chen, Athziry Paz, Jude Savarraj, Han-Gil Jeong, Moon-Ku Han, Aaron Gusdon, Xuefang Sophie Ren, Spiros Blackburn, Huimahn Alex Choi Aug 2025

Targeted Proteomic Analysis Identifies Acvrl1 As A Marker Of Cerebral Edema In Aneurysmal Subarachnoid Hemorrhage, Bosco Seong Kyu Yang, Lena O'Keefe, Sarah Hinds, Hua Chen, Athziry Paz, Jude Savarraj, Han-Gil Jeong, Moon-Ku Han, Aaron Gusdon, Xuefang Sophie Ren, Spiros Blackburn, Huimahn Alex Choi

Faculty, Staff and Student Publications

Cerebral edema (CE) is a major component of early brain injury from aneurysmal subarachnoid hemorrhage (SAH). Despite the utility of blood-based protein markers, biomarkers targeting cerebral edema have yet to be developed. We performed a targeted proteomic search analyzing 141 proteins in plasma samples taken from 80 adult patients within 48 hours after aneurysmal rupture to identify plasma biomarkers of cerebral edema. The protein expression profiles were analyzed for associations with a higher level of the subarachnoid hemorrhage early brain edema score measured in admission computed tomography scans. Five differentially expressed proteins-brorin, ectodysplasin A2 receptor, layilin, scavenger receptor class A …

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High Abundances Of Crustose Coralline Algae Inside Cryptic Coral Habitats Linked To Coral Reef Functioning, Anna Rothstein, Orion S. Mccarthy, Sophie Luu, Emily L.A. Kelly, Philip Heller, Stuart Sandin, Jennifer E. Smith, Maya S. Devries Aug 2025

High Abundances Of Crustose Coralline Algae Inside Cryptic Coral Habitats Linked To Coral Reef Functioning, Anna Rothstein, Orion S. Mccarthy, Sophie Luu, Emily L.A. Kelly, Philip Heller, Stuart Sandin, Jennifer E. Smith, Maya S. Devries

Faculty Research, Scholarly, and Creative Activity

Cryptic habitats on coral reefs consist of crevices, tunnels, and holes that are estimated to comprise 30%–75% of the total reef surface area. These large habitats are vastly understudied because they are often difficult to access. Crustose coralline algae (CCA) are thought to perform important ecological functions, including calcium accretion, both inside cryptic habitats and on the exposed (top) reef. Using GoPro cameras, we surveyed 250 coral crevices from 13 reefs on West Maui, Hawai‘i. We compared relative CCA cover between crevice microhabitats and the well-studied top-reef and identified abiotic and biotic factors that correlated with CCA abundance. We found …

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Late Holocene Paleodietary Patterns Among The Ancestral Ohlone: Ecogeographic Partitioning Of Resources Along The San Francisco Bay Eastern Shore, Melanie M. Beasley, Eric J. Bartelink, Alan Leventhal, Monica V. Arellano, Richard Massiatt, Charlene Nijmeh Aug 2025

Late Holocene Paleodietary Patterns Among The Ancestral Ohlone: Ecogeographic Partitioning Of Resources Along The San Francisco Bay Eastern Shore, Melanie M. Beasley, Eric J. Bartelink, Alan Leventhal, Monica V. Arellano, Richard Massiatt, Charlene Nijmeh

Faculty Research, Scholarly, and Creative Activity

Objectives: In the San Francisco Bay Area, Late Holocene resource intensification models predict an increase in diet breadth and a reduction in foraging efficiency associated with an increase in population, sedentism, and territoriality among fisher-hunter-gatherer populations. Here we compare bone stable carbon (δ13C) and nitrogen (δ15N) isotope values of sites along the eastern bay shore to interpret how ecogeographical partitioning of resources by latitude and distance from the bay reflects differential access and control of resources. Materials and Methods: With the support of, and in collaboration with, the Muwekma Ohlone Tribe of the San Francisco Bay …

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Validated Hots-Based Worksheets Enhance Higher-Order Thinking Skills And Impact Geometry Learning Outcomes, Aulia Al Tsani Izzati, Siti Faizah, Mardhatillah Mardhatillah Aug 2025

Validated Hots-Based Worksheets Enhance Higher-Order Thinking Skills And Impact Geometry Learning Outcomes, Aulia Al Tsani Izzati, Siti Faizah, Mardhatillah Mardhatillah

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

This study aims to develop and evaluate worksheets that promote higher-order thinking skills (HOTS) in teaching perimeter and area to primary school students. The worksheets were highly valid, with a score of 94.63%, and were shown to be well aligned with learning objectives through extensive validity and reliability testing. They contain various question types, including multiple-choice, short-answer and essay questions, which encourage students to think critically and creatively. While many students improved their analytical skills, 39% needed to meet the minimum competency standard (KKM). This underscores the necessity for supplementary strategies to support all learners and enhance learning outcomes.

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Enhancing Student Engagement And Cognitive Development Through Game-Based Learning (Gbl), Kusuma Rani, Putri Dwi Lestari, Saenab Ka-Lupae Aug 2025

Enhancing Student Engagement And Cognitive Development Through Game-Based Learning (Gbl), Kusuma Rani, Putri Dwi Lestari, Saenab Ka-Lupae

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

Elementary school students are classified as playing-age children, they like to do activities while playing. This research aims to determine the influence of the Game-Based Learning (GBL) learning model on students' cognitive activities and abilities. This type of research is quasi-experimental, one-group pre-test and post-test design. The population of this study were Madrasah Ibtidaiyah students at Darussalam Seuksa School, Thepa, Southern Thailand. The research sample for grade 5 students was 28 students. Student activity is measured using an observation sheet with indicators measured using a 1-5 Likert scale. Cognitive abilities are measured by tests. The results of data analysis showed …

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Leading And Learning From Inside Out: Insights From Meditative Inquiry, Jody L. Rebek Aug 2025

Leading And Learning From Inside Out: Insights From Meditative Inquiry, Jody L. Rebek

Journal of Contemplative and Holistic Education

This article explores our modern world and our role in shaping collective experiences. By our thoughts, choices and behaviours (our character), we influence our shared reality. Higher education has an imperative responsibility to engage students ethically and develop their character. I offer a teaching metaphor and case study to meaningfully develop students’ self-awareness of character via an undergraduate leadership course. I employed the ‘seed’ metaphor to illustrate how nurturing students’ self-awareness and agency mirrors the growth of a seed within a carefully cultivated ecosystem. Each contemplative practice (character development) intertwined with leadership content (competence and confidence), serves as essential nourishment, …

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Font Size Adjustment Based On Ambient Light And User Profile, Sahil Gupta Aug 2025

Font Size Adjustment Based On Ambient Light And User Profile, Sahil Gupta

Defensive Publications Series

Reading text on electronic device displays can be difficult in varying ambient light conditions, particularly for users with age-related vision changes. Current solutions require manual font size adjustments, which are static, cumbersome, and separate from automatic brightness controls. This disclosure describes a method for adjusting the font size on a display in response to changing conditions, without requiring explicit user interaction. For example, an ambient light sensor may measure surrounding light levels, and user-specific data, such as age and/or a vision quality rating, can be optionally provided. An algorithm uses these inputs to determine an appropriate font size in real …

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Possible Cross-Resistance And Horizontal Gene Transfer Associated With Halquinol In Escherichia Coli And Salmonella Enterica Isolated From Pigs, Nisit Chansong, Songsak Srisanga, Nutthavut Mahawatthanaunkul, Rungtip Chuanchuen Aug 2025

Possible Cross-Resistance And Horizontal Gene Transfer Associated With Halquinol In Escherichia Coli And Salmonella Enterica Isolated From Pigs, Nisit Chansong, Songsak Srisanga, Nutthavut Mahawatthanaunkul, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

The aims of this study were to determine the possible cross-resistance and horizontal gene transfer associated with halquinol in Escherichia coli and Salmonella spp. from pigs. Two of each E. coli and Salmonella isolates exhibiting low halquinol MIC (8 and 16 µg/ml, respectively) and susceptible to antibiotics tested were selected for in vitro stepwisehalquinol exposure experiment. Another E. coli (n=19) and Salmonella (n=6) isolates with high halquinol MICs (16-64 and 32-64 µg/ml, respectively) containing plasmids were used for transferability testing of halquinol resistanceencoding gene(s) using filter mating method. In vitro exposure to subinhibitory concentrations of halquinol promoted neither resistance to …

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Unmasking The Shadows: A Comprehensive Exploration Of Serial Killers, Erica D. Lewis Aug 2025

Unmasking The Shadows: A Comprehensive Exploration Of Serial Killers, Erica D. Lewis

Master of Arts in Human Services

Using a mixed-methods approach that combines quantitative literature review and qualitative case study analysis, this capstone investigates the developmental pathways of serial killers. The study investigates a variety of biological, psychological, and sociological elements—such as genetic predispositions, neurological disorders, childhood traumas, and environmental stressors—interact to shape serial killers. The study offers a multidisciplinary perspective, grounded in criminological models such as routine activity and labeling theories, sociological frameworks such as social disorganization and strain theory, and psychological theories such as psychopathy and attachment theory. Theoretical findings are contextualized through case studies of notorious serial killers like Ted Bundy, Jeffrey Dahmer, and …

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Designing Writers: A Self-Regulated Approach To Multimodal Composition In Teacher Preparation And Early Grades, Qi Si, Tracey S. Hodges, Vahid Mousavi Aug 2025

Designing Writers: A Self-Regulated Approach To Multimodal Composition In Teacher Preparation And Early Grades, Qi Si, Tracey S. Hodges, Vahid Mousavi

Teacher Education and Leadership Faculty Publications

Reading and writing in the 21st century have evolved from traditional text-based formats to multimodal literacy, integrating linguistic, visual, auditory, and spatial modes to enhance communication and comprehension. While multimodal reading has been widely studied, multimodal writing remains underexplored, despite its growing importance in K–12 education across disciplines. Multimodal composing demands advanced self-regulation as students navigate multiple digital tools and platforms. Self-regulated learning strategies, particularly the self-regulated strategy developmental model, offer a promising approach to support students in planning, monitoring, and revising multimodal compositions. However, a comprehensive framework linking self-regulation and multimodal composition is lacking. This article addresses this gap …

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State Of Illinois Cannabis Testing, University Of Mississippi – National Center For Cannabis Research And Education, Yasha Kahn, Mcr Labs Aug 2025

State Of Illinois Cannabis Testing, University Of Mississippi – National Center For Cannabis Research And Education, Yasha Kahn, Mcr Labs

Illinois

No abstract provided.

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Efficient Decontamination Of Organophosphate-Based Pesticides And Nerve Agent Simulants Mediated By N-Containing Nucleophiles, Emmanuel Kingsley Darkwah, Puspa Aryal, Saidulu Todeti, Jared Kenik, Samuel Insall, V. Prakash Reddy, Chariklia Sotiriou-Leventis Aug 2025

Efficient Decontamination Of Organophosphate-Based Pesticides And Nerve Agent Simulants Mediated By N-Containing Nucleophiles, Emmanuel Kingsley Darkwah, Puspa Aryal, Saidulu Todeti, Jared Kenik, Samuel Insall, V. Prakash Reddy, Chariklia Sotiriou-Leventis

Chemistry Faculty Research & Creative Works

There is a growing interest in developing safer and more effective decontaminating agents for organophosphate-based pesticides and nerve agents. In this study, we present an effective method for the nonaqueous decontamination of these compounds using small-molecule-based decontaminating agents under ambient conditions. Our approach utilizes aryl and heteroaryl carboxaldehyde hydrazones and hydrazides to effectively hydrolyze nerve agent simulants into their nontoxic degradation products. The effectiveness of this method was evaluated using a range of nerve agent simulants, including dimethyl 4-nitrophenyl phosphate (DMNP), dimethyl methyl phosphonate (DMMP), and triphenyl phosphate (TPhP). In the presence of the heteroaryl hydrazone, the rate of hydrolysis …

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Enhanced Meropenem Activity And Stability Following Load In Polyvinyl Alcohol Nanofiber Scaffolds With Sitagliptin As Quorum Sensing Inhibitor On Pseudomonas Aeruginosa, Samar A. Salim, Abdelrhman Mohsen, Mohamed Emara, Taghrid Elmahdy Aug 2025

Enhanced Meropenem Activity And Stability Following Load In Polyvinyl Alcohol Nanofiber Scaffolds With Sitagliptin As Quorum Sensing Inhibitor On Pseudomonas Aeruginosa, Samar A. Salim, Abdelrhman Mohsen, Mohamed Emara, Taghrid Elmahdy

Nanotechnology Research Centre

Background The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic efficacy without inhibiting bacterial growth, minimizing the risk of resistance. Aims Evaluate the combined effect of Sitagliptin (STG) as a QSI with Meropenem (MER), fabricate drug-loaded Polyvinyl Alcohol (PVA) nanofibers, and investigate their antimicrobial activity against standard Pseudomonas aeruginosa (PAO1) and carbapenem-resistant P. aeruginosa (CRPA). Methods The combinatorial effect was assessed using a checkerboard assay. PVA/STG, PVA/MER, and PVA/STG/ MER nanofibers were fabricated …

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Exposure To Zinc Oxide Nanoparticles Reveals A Death Resistance Mechanism In Dictyostelium Discoideum, Zoya Nadeem Faruqui Aug 2025

Exposure To Zinc Oxide Nanoparticles Reveals A Death Resistance Mechanism In Dictyostelium Discoideum, Zoya Nadeem Faruqui

Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs) are very versatile due to their multifunctional properties including unique physicochemical and antimicrobial characteristics. They are widely utilized across various fields such as biomedical, industrial fields, environmental remediation, and agriculture. Therefore, their cytotoxic effects on biological systems necessitate a deeper understanding of the cellular responses to nanoparticle exposure. This project primarily investigates the impact of ZnO NPs on the model organism Dictyostelium discoideum AX4 (social amoeba) which has not been examined before unlike a vast variety of organisms in the literature. The cytotoxic effects of ZnO NPs on Dictyostelium discoideum AX4 cells are compared with …

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Three Essays In Gamification, Innovation, And Cybersecurity, Eduard Yelagin Aug 2025

Three Essays In Gamification, Innovation, And Cybersecurity, Eduard Yelagin

Electronic Theses and Dissertations Archive

This dissertation consists of three essays on Gamification, Innovation, and Cyber Security. The first essay investigates gamified stock trading. Gamification of stock trading is a novel practice by brokers to incorporate game-like features to increase clients’ engagement with trading. This study examines how the market reacts to the introduction of 142 gamification features in the mobile trading apps of 17 major U.S. brokers. I find that the gamification of trading can be viewed as a double-edged sword. It alters and worsens retail traders’ strategy by reducing their returns and increasing return volatility. However, it also reduces costs and risks for …

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The Intersection Of Social Groups And The Self, Zachary Robert Auwerda Aug 2025

The Intersection Of Social Groups And The Self, Zachary Robert Auwerda

Electronic Theses and Dissertations Archive

This dissertation explores the nature of social identity by examining both the collective identity of groups and the social selves of individuals. I start by offering a Humean model of collective identity. On this model, the metaphysical nature of group persistence depends on our psychological dispositions and our explanatory goals. On this view, collective identity is a fiction—something that humans ascribe to a group—rather than something that exists independently. I argue that this Humean model has advantages over structural and intentional theories of group persistence. I then move onto the topic of social construction and its incompatibility with methodological individualism. …

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Impacts Of Climate Change On Vertical Migration Of Zooplankton Species, Camila Rimoldi Ibañez Aug 2025

Impacts Of Climate Change On Vertical Migration Of Zooplankton Species, Camila Rimoldi Ibañez

Florida Atlantic Undergraduate Research Journal

Vertical migration in marine and freshwater environments stands as one of Earth’s largest daily animal movements concerning biomass. It serves as a crucial life pump, shaping phytoplankton grazing patterns, facilitating nutrient transport through the water column, and providing vital sustenance for deep-water organisms lacking access to surface phytoplankton. Despite enduring millennia of climatic shifts, contemporary anthropogenic climate change surpasses historical rates of change, posing unprecedented challenges. Climate-induced phenomena increasingly affect various species, including vertical migrators. This paper delves into Diel Vertical Migration’s ecological significance and anticipates detrimental effects of climate change on individual animals’ physiology and migration patterns. Foremost, rising …

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Exploring Infant Toy Interactions In Free Play Environments, Emma Bermudez, Jacqueline Hammack Aug 2025

Exploring Infant Toy Interactions In Free Play Environments, Emma Bermudez, Jacqueline Hammack

Florida Atlantic Undergraduate Research Journal

Infants’ interactions with toys play a crucial role in their early cognitive and motor development, yet much of the research on this topic has been conducted in controlled laboratory settings, which may not accurately reflect the diversity of toys and interactions infants experience in their natural environments. This study uniquely examined these interactions in a home setting by utilizing an online video conferencing platform. This study investigated how toy properties influence manual infant-object interactions during dyadic tabletop free play, where mothers selected the toys for the session. Within the confines of this study, results show that infants spend significantly more …

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Use Of A Novel Plant Essential Oil-Based Insecticide For Insect Control In Household Gardens And Lawns, Mia Michnik, Alysa Suissa, Liah Brussolo Cremona, Annalise Wellman, Nicholas Nifakos, Alexander Spanoudis, John M. Harlin Aug 2025

Use Of A Novel Plant Essential Oil-Based Insecticide For Insect Control In Household Gardens And Lawns, Mia Michnik, Alysa Suissa, Liah Brussolo Cremona, Annalise Wellman, Nicholas Nifakos, Alexander Spanoudis, John M. Harlin

Florida Atlantic Undergraduate Research Journal

Insecticide use has increased as population and agricultural demand for crops grows. Commercial insecticides contain active ingredients that are often harmful to humans and the environment. A viable alternative to this concern is developing and using insecticides with low toxicity and minimum risk to the environment. In this study, we sought to empirically assess the effectiveness of an insecticide based entirely on plant essential oil active ingredients, which contain complex secondary metabolites. Our results show that a concoction of several plant essential oils was effective in killing a wide range of household garden insect species and bedbugs, encompassing five orders …

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Artist Statement, Shreyansh Patel Aug 2025

Artist Statement, Shreyansh Patel

Florida Atlantic Undergraduate Research Journal

No abstract provided.

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Plant-Microbe Interactions Across Scales: Diversity, Disease, And Stress In Herbaceous Plant Communities, Philippa Tanford Aug 2025

Plant-Microbe Interactions Across Scales: Diversity, Disease, And Stress In Herbaceous Plant Communities, Philippa Tanford

Arts & Sciences Graduate Student Theses and Dissertations

Plants interact consequentially with microbes spanning mutualism to pathogenicity, with broader effects on large-scale plant community structure. These plant communties, in turn, shape below and aboveground microbial communities through a variety of mechanisms. However, plant diversity-microbial diversity relationships remain broadly inconsistent across environments and among different plant and microbial assemblages. In my dissertation, I work to bridge some of these gaps in plant-microbial ecology in three experimental chapters assessing relationships between plant diversity and microbial diversity, including the influence of belowground-aboveground microbiome interactions, the influence of drought stress, and incorporating different spatial and experimental scales. First, I explore the microbial …

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Tradition, Critque, And The Modern Moral Lingua Franca: A Lutheran Articulation Of Human Rights Language, Christian B. Dollar Aug 2025

Tradition, Critque, And The Modern Moral Lingua Franca: A Lutheran Articulation Of Human Rights Language, Christian B. Dollar

Doctor of Philosophy Dissertation

The language of subjective, individual rights has become the primary way of speaking about the rights and wrongs of human life together. Since the end of World War II, one subset of this language—that of human rights—has achieved a global influence. The various Christian traditions of the west have, in large part, adopted this language of human rights to articulate and supplement their assorted social teachings. Yet, the adoption of human rights language has been contested by a dedicated cadre of Roman Catholic and Protestant theologians concerned about the potentially anti-Christian implications inherent in the grammar of rights. Christian rights …

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Man Bites Dog: Indian Supreme Court Conundrum, Andrew Rowan Dphil, Kathleen Rowan Aug 2025

Man Bites Dog: Indian Supreme Court Conundrum, Andrew Rowan Dphil, Kathleen Rowan

WellBeing News

This article analyzes the Indian Supreme Court’s suo motu order to remove stray dogs from New Delhi streets after a fatal rabies case. The decision departs from prior rulings, highlighting a shift toward public safety over precedent.

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Defining Animal Welfare, Andrew Rowan Dphil Aug 2025

Defining Animal Welfare, Andrew Rowan Dphil

WellBeing News

Animal welfare is framed as an animal’s ability to cope with its environment, based on Broom’s 1986 definition. The article critiques productivity-based measures and highlights the Welfare Footprint as a metric for quantifying animals’ lived experiences.

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Experience-Dependent Intrinsic Plasticity In Layer Iv Of Barrel Cortex At Whisking Onset, Molly C Shallow, Lucy Tian, Bryan T Higashikubo, Hudson Lin, Katheryn B Lefton, Siyu Chen, Joseph D Dougherty, Joe P Culver, Mary E Lambo, Keith B Hengen Aug 2025

Experience-Dependent Intrinsic Plasticity In Layer Iv Of Barrel Cortex At Whisking Onset, Molly C Shallow, Lucy Tian, Bryan T Higashikubo, Hudson Lin, Katheryn B Lefton, Siyu Chen, Joseph D Dougherty, Joe P Culver, Mary E Lambo, Keith B Hengen

2020-Current year OA Pubs

The development of motor control over sensory organs is a critical milestone, enabling active exploration and shaping of the sensory environment. Whether the onset of sensory organ motor control directly influences the development of corresponding sensory cortices remains unknown. Here, we confirm and exploit the late onset of whisking behavior in mice to address this question in the somatosensory system. Using ex vivo electrophysiology, we describe a transient increase in the intrinsic excitability of excitatory neurons in layer IV of the barrel cortex, which processes whisker input, immediately following the onset of active whisking on postnatal days 13 and 14. …

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Corticotropin-Releasing Hormone Modulates Nrem Sleep Consolidation Through The Thalamic Reticular Nucleus, Loredana Cumpana, Olivia Zanoletti, Dinesh Kankanamge, Bryan Copits, Carmen Sandi, Simone Astori Aug 2025

Corticotropin-Releasing Hormone Modulates Nrem Sleep Consolidation Through The Thalamic Reticular Nucleus, Loredana Cumpana, Olivia Zanoletti, Dinesh Kankanamge, Bryan Copits, Carmen Sandi, Simone Astori

2020-Current year OA Pubs

Corticotropin-releasing hormone (CRH) is a peptide associated with stress and anxiety that acts as a potent modulator throughout the nervous system. The thalamic reticular nucleus (TRN) displays high expression of the CRH receptor 1 (CRHR1), but whether CRH modulates key TRN functions, such as sleep spindle rhythmogenesis, remained unexplored. Combining polysomnographic and photometric recordings in mice, we show that CRH release in TRN during non-rapid-eye movement sleep (NREMS) oscillates with a ~50-s periodicity, anti-correlating with sleep spindle dynamics. Optogenetic manipulations of CRH release in TRN modulated NREMS fragmentation through microarousals with corresponding changes in sigma and delta power. In ex-vivo …

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O-Glcnac Transferase Plays Dual Antiviral Roles By Integrating Innate Immunity And Lipid Metabolism, Hong Dong, Yong Zhang, Michael J Holtzman, Et Al. Aug 2025

O-Glcnac Transferase Plays Dual Antiviral Roles By Integrating Innate Immunity And Lipid Metabolism, Hong Dong, Yong Zhang, Michael J Holtzman, Et Al.

2020-Current year OA Pubs

Viral infection induces robust reprogramming of metabolic pathways in host cells. However, whether host metabolic enzymes detect viral components remains unknown. Our group and others previously identified O-GlcNAc transferase (OGT), an important glucose metabolic enzyme, as a crucial mediator of the antiviral immune responses. Here, by studying a mouse model with a catalytically impaired OGT, we discover a catalytic activity-independent function of OGT in restraining influenza A virus (IAV) infection in addition to its catalytic activity-dependent effect on MAVS-mediated antiviral immunity. Biochemical studies reveal a critical antiviral effect based on OGT interacting with IAV genomic RNA that requires its N-terminal …

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Echocardiographic Assessment Of Pulmonary Hemodynamics And Right Ventricular Performance In Neonatal Murine Hypoxia, Kel Vin Woo, Philip T Levy, Carla J Weinheimer, Amanda L Hauck, Aaron Hamvas, David M Ornitz, Attila Kovacs, Gautam K Singh Aug 2025

Echocardiographic Assessment Of Pulmonary Hemodynamics And Right Ventricular Performance In Neonatal Murine Hypoxia, Kel Vin Woo, Philip T Levy, Carla J Weinheimer, Amanda L Hauck, Aaron Hamvas, David M Ornitz, Attila Kovacs, Gautam K Singh

2020-Current year OA Pubs

No abstract provided.

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Bcg Vaccine-Induced Innate And Adaptive Pulmonary Immunity Correlating With Protective Efficacy Against Mycobacterium Tuberculosis In The Lungs, Mayank Khanna, Alistair J. Ramsay Aug 2025

Bcg Vaccine-Induced Innate And Adaptive Pulmonary Immunity Correlating With Protective Efficacy Against Mycobacterium Tuberculosis In The Lungs, Mayank Khanna, Alistair J. Ramsay

School of Graduate Studies Faculty Publications

Background/Objectives: Effective prophylaxis for Mycobacterium tuberculosis (Mtb) requires greater understanding of immune correlates of protection. With renewed interest in BCG as an Mtb vaccine, particularly via the intravenous (IV) route, our objective was to characterize both innate and adaptive immune correlates of vaccine-induced pulmonary immunity as potential biomarkers for protective efficacy in a murine model of Mtb infection. Methods: Mice were given BCG via different routes and some boosted with recombinant virus constructs encoding Mtb Ag85B. Responding innate lymphoid cell (ILC) populations, T cells and B cells were analyzed by fluorescence activated cell sorting (FACS) for surface markers and by …

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