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Soil And Tree Nutrient Status Of High Elevation Mixed Red Spruce (Picea Rubens Sarg.) And Broadleaf Deciduous Forests, Phillip M. Crim, Louis M. Mcdonald, Jonathan R. Cumming

Faculty & Staff Scholarship

Abstract: Anthropogenic and industrial emissions have resulted in historically high levels of acidic deposition into central Appalachian forests. Despite the reduction in acidic inputs due to legislation curbing industrial emissions in the United States, continued N deposition may impact forest ecosystems. Soil and foliar samples were collected from four high elevation red spruce sites along a modeled gradient of historic N deposition. The three most abundant tree species at all sites, Acer rubrum L., Betula alleghaniensis Britt., and Picea rubens Sarg., were sampled. Bulk soil beneath the canopies of individual trees were collected from the top 15-cm and separated into …

Characterization And Quantification Of Major Flavonol Glycosides In Ramps (Allium Tricoccum), Wijdan M. Dabeek, Nik Kovinich, Callee Walsh, Melissa Ventura Marra

Faculty & Staff Scholarship

The ramp (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in traditional medicine for their health-promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of the potentially bioactive components in ramps is limited. Therefore, the aim of this work was to characterize and quantify major flavonols in ramps. Flavonoids were extracted in 50% methanol and 3% acetic acid. Characterization was conducted using UHPLC-PDA-MS and MS/MS, and quantification was performed using UHPLC-PDA detection. The major flavonol glycosides were kaempferol sophoroside glucuronide, quercetin sophoroside glucuronide, kaempferol rutinoside glucuronide, quercetin hexoside glucuronide, …

Exercise Training Prevents The Perivascular Adipose Tissue-Induced Aortic Dysfunction With Metabolic Syndrome, Evan Devallance, Kayla W. Branyan, Kent C. Lemaster, Ray Anderson, Kent L. Marshall, I. Mark Olfert, David M. Smith, Eric E. Kelly, Randy W. Bryner, Jefferson C. Frisbee, Paul D. Chantler

Faculty & Staff Scholarship

The aim of the study was to determine the effects of exercise training on improving the thoracic perivascularadipose tissue (tPVAT) phenotype (inflammation, oxidative stress, and proteasome function) in metabolic syn-drome and its subsequent actions on aortic function.Methods:Lean and obese (model of metabolic syndrome) Zucker rats (n=8/group) underwent 8-weeks ofcontrol conditions or treadmill exercise (70% of max speed, 1 h/day, 5 days/week). At the end of the inter-vention, the tPVAT was removed and conditioned media was made. The cleaned aorta was attached to a forcetransducer to assess endothelium-dependent and independent dilation in the presence or absence of tPVAT-conditioned media. tPVAT gene …

Transcutaneous Electrical Nerve Stimulation (Tens) For Chronic Pain - An Overview Of Cochrane Reviews (Review), William Gibson, Benedict M. Wand, Catherine Meads, Mark J. Catley, Neil E. O'Connell

Physiotherapy Papers and Journal Articles

Background: Chronic pain, considered to be pain lasting more than three months, is a common and often difficult to treat condition that can significantly impact upon function and quality of life. Treatment typically includes pharmacological and non-pharmacological approaches. Transcutaneous electrical nerve stimulation (TENS) is an adjunct non-pharmacological treatment commonly recommended by clinicians and often used by people with pain.

Objectives: To provide an overview of evidence from Cochrane Reviews of the effectiveness of TENS to reduce pain in adults with chronic pain (excluding headache or migraine). To provide an overview of evidence from Cochrane Reviews of the safety of …

Early Care And Education Testimonios At The Borderlands, Jennifer Torres Siders

University of the Pacific Theses and Dissertations

Latinas represent a large proportion of the United States early care and education workforce, and thus have the potential to wield significant influence over the growth and development of millions of American children. However, the voices of Latina early childhood professionals often are missing in both research and mass media. Instead, social, political, and academic frames cast Latinas as foreign regardless of nationality, uneducated notwithstanding expertise, and passive despite action and influence. This testimonio analysis draws on Chicana feminist epistemology to re-center the perspectives of Latina child care providers and reveal more authentic insights on how they understand and perform …

Intrapulmonary Inoculation Of Multicellular Tumor Spheroids To Construct An Orthotopic Lung Cancer Xenograft Model That Mimics Four Clinical Stages Of Non-Small Cell Lung Cancer, Yingbo Huang

University of the Pacific Theses and Dissertations

Lung cancer leads in mortality among all types of cancer in the US and Non-small cell lung cancer (NSCLC) is the major type of lung cancer. Immuno-compromised mice bearing xenografts of human lung cancer cells represent the most common animal models for studying lung cancer biology and for evaluating potential anticancer agents. However, orthotopic lung cancer models based on intrapulmonary injection of suspended cancer cells feature premature leakage of the cancer cells to both sides of the lung within five days, which generates a quick artifact of metastasis and thus belies the development and progression of lung cancer as seen …

Characterization And Analysis Of Proteins Secreted By The Mutant Pichia Pastoris Strain, Bgs13, Christopher Alan Naranjo

University of the Pacific Theses and Dissertations

The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for research, clinical, and industrial purposes to produce thousands of recombinant protein products. Because P. pastoris secretes very few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, a disadvantage to the programmed export is that some recombinant proteins intended for secretion are retained within the cell and may be subsequently degraded. A mutant strain isolated in our lab, containing a pREMI-derived disruption of the BGS13 gene, has displayed elevated levels of secretion for a variety …

Effects Of Eye Tracking During Infancy On Social Ambiguity And Emotional Distress, Gabriela Hernandez

University of the Pacific Theses and Dissertations

The purpose of this study was to investigate the relationship between early eye tracking and later non-suicidal self-injury, and whether this relationship was negatively impacted by distress and impulsivity, and conversely alleviated by emotional regulation and problem solving abilities. Briggs-Gowan et al. (2001) found that young children with recurrent and comorbid externalizing and internalizing problems have the most impairment; they exhibit greater difficulty with emotional stability, and require greater utilization of mental health services. Little research has focused on the relationship between eye tracking in early infancy and suicidal self-injurious (NSSI) behavior in early childhood. Multiple regression analysis was used …

Applicability Of The Dual Isotopes Δ15n And Δ18o To Identify Nitrate In Groundwater Beneath Irrigated Cropland, R. F. Spalding, A. J. Hirsh, M. E. Exner, N. A. Little, K. L. Kloppenborg

Department of Agronomy and Horticulture: Faculty Publications

Identification of the nitrate sources that adversely impact groundwater quality is a necessary first step in the control of this major worldwide pollutant. The impact of nitrate leachate from urea-ammonium nitrate (UAN) (50% urea-N, 25% ammonium-N, 25% nitrate-N) fertilizer, whose use has increased dramatically in the last three decades largely because it can be applied through sprinkler irrigation systems to corn in all growth stages, is investigated. The dual isotopes δ¹⁵N_NO3 and δ¹⁸O_NO3 were measured in groundwater samples from 39 irrigation wells in two intensively sprinkler-irrigated, corn-growing areas of Nebraska with nitrate-contaminated (N …

Restoring Fire-Grazer Interactions To Pursue Heterogeneity In Sandhills Prairie, Jack R. Arterburn, Dirac L. Twidwell Jr, Carissa L. Wonkka, Walter H. Schacht, David A. Wedin

Department of Agronomy and Horticulture: Faculty Publications

Heterogeneity has emerged as a fundamental principle for grassland management and the importance of environmental heterogeneity for biological diversity has raised questions about the appropriateness of grassland practices that seek to promote uniform grassland structure and composition. Principles of uniformity in grassland management reflect a utilitarian target of “managing for the middle” by minimizing both overgrazing and underutilization while avoiding or preventing fire and other disturbances that consume aboveground biomass. We evaluated pioneering efforts to restore fire-grazer interactions via patch burning in an effort to increase spatial heterogeneity at the patch scale in the Nebraska Sandhills, a sandy soil, mixed-grass …

Recolonization Of Mollusc Assemblages In Mangrove Plantations Damaged By Typhoon Chan-Hom In The Philippines, Severino G. Salmo Iii, Ian R. Tibbetts, Norman C. Duke

Environmental Science Faculty Publications

We investigated the effects of a catastrophic typhoon on mollusc assemblages of damaged mangrove plantations of different ages. Molluscs were sampled from infaunal, epifaunal and arboreal assemblages of mangrove stands in Lingayen Gulf, northwest Philippines, and compared with assemblages of un-impacted areas. Prior to the occurrence of the typhoon, there were clear shifts in the species diversity (H’) and composition of mollusc assemblages with stand age of mangrove forests. This was observed in species composition through the succession in dominance from pioneer to seral or putative climax species, and assemblage type (as arboreal, epifaunal and infaunal). However, severe damage to …

Effects Of Gardenia Jasminoides Fruit Extract On Helicobacter Pylori Induced Gastritis In Vitro And In Vivo Studies, Nattida Noonak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Helicobacter pylori (H. pylori) releases virulence factors to gastric epithelial cells causing an imbalance between aggressive factors and protective factors leading to gastritis inflammation. Gardenia jasminoides (G. jasminoides) fruit extract contains geniposide as a major bioactive compound that possesses anti-inflammatory effects. The present study aimed to investigate the effects of G. jasminoides fruit extract on gastric inflammation and protective mechanisms in H. pylori-induced gastritis. In an in vitro experiment, we demonstrated that G. jasminoides fruit extract could inhibit growth of H. pylori using disk diffusion method. The result showed that the inhibition zone was observed at geniposide ≥ 0.16 mg/disc. …

A Kentucky Pioneer In Music Therapy: An Oral History On The Life And Career Of Lorinda Jones, Emma Powers

Theses and Dissertations--Music

Lorinda Jones, MT-BC, is the longest practicing music therapist in Kentucky. She began her work as a music therapist in 1995 and built a private practice, which expanded over the course of the next 20 years to include services in 16 counties. Ms. Jones’ perspective on the growth of music therapy, both within the state and nationwide, as well as her extensive knowledge of Appalachian folk music, makes her an invaluable resource to Kentucky music therapists. The purpose of this study was to present a historical account of the life and career of Lorinda Jones, to gain her perspective on …

Finding The “Tech” In Technique: A Pedagogical Approach To Electroacoustic Concert Percussion Performance Practice, William Brady Harrison Ii

Theses and Dissertations--Music

Premise and Objectives

In our increasingly technology driven society, the impact of technology touches nearly every aspect of our lives in some form or another. This has been acutely felt within the world of percussion, with electroacoustic works representing perhaps the most rapidly expanding area of concert percussion over the last twenty years. Electroacoustic music couples electronic technology with traditional acoustic instruments and/or performance practices.

Broadly, this paper outlines a systematic approach to teaching electroacoustic performance practice, based on elements found in a cross-section of percussion literature. In using such an approach, not only does each student become more capable …

When The Inhuman Becomes Human: An Examination Of The Musical Portrayal Of The Robot In Twenty-First Century Science-Fiction Cinema Through An Analysis Of The Film Scores Of Automata, Ex Machina, And The Machine, Rebecca Ann O'Brien

Theses and Dissertations--Music

Science fiction film has been telling stories about artificial anthropomorphic robots and androids for almost a hundred years, spawning films, such as Metropolis (1927), Ghost in the Shell (1951), and Blade Runner (1982). Each of these science-fiction films was complemented by a musical score that helped to create an onscreen world dominated by a dystopian view of the future. Influenced by the generations of prior science-fiction films, Automata (2014), The Machine (2013), and Ex Machina (2015) are all concerned with the same narrative in which humanity is in decline while artificial robots are rising up and experiencing life in a …

Do Teachers Participating In A Therapy Dog Program Perceive An Impact On Students’ Attitudes And Behaviors Toward Reading?, Jenna M. Dragani-Reagan

PCOM Psychology Dissertations

No abstract provided.

Sleep, Communicative Ability, And Diet As Predictors Of Aggression In Autism Spectrum Disorder, Rafat Omar

PCOM Psychology Dissertations

Aggression is a common behavioral issue in children with autism spectrum disorder (ASD). Aggression is often treated with evidence-based behavioral treatments, such as applied behavioral analysis and functional communication training. Another form of intervention that has grown in popularity is to alter the child’s diet. The most popular dietary intervention, the autism diet, has been used as a form of behavior management by parents and guardians for children diagnosed with ASD to address aggressive behavior; however, its use has not been strongly supported by research. The authors proposed that a more beneficial way of understanding aggression in ASD is to …

Sensory Processing Patterns And Emotion Regulation In Children Presenting With Externalizing Behaviors, Melanie Levitt

PCOM Psychology Dissertations

Sensory processing is a neurological process that involves a child’s perception, organization, and reaction to sensory stimuli. Certain groups of people, such as children diagnosed with Autism Spectrum Disorder (ASD) and Attention-Deficit/Hyperactivity Disorder (ADHD), experience more intense sensory processing patterns (Dunn, 2007). Researchers have also observed that children presenting with ASD and ADHD exhibit more externalizing scores as their sensory processing patterns become more intense or problematic (Gourley et al., 2013). However, researchers have not examined the relationship between sensory processing and externalizing behaviors in those not diagnosed with ASD and ADHD. Additionally, there are overlapping neural mechanisms between sensory …

Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Food packaging technology has been advancing to provide safe and high quality food products and to minimize food waste. Moreover, there is a dire need to replace plastic materials in order to reduce environmental pollution. The aim of this study was to prepare biodegradable antimicrobial packaging films from gelatin. Boric acid, disodium octaborate tetrahydrate, and sodium pentaborate were incorporated as the antimicrobial agents. Films containing boric acid and its salts showed antibacterial effect against Staphylococcus aureus and Pseudomonas aeruginosa, as well as antifungal and anticandidal effects against Aspergillus niger and Candida albicans. The mechanical strength of the films was mostly …

Prediction And Expression Analysis Of G Protein-Coupled Receptors In The Laboratory Stick Insect, Carausius Morosus, Burçi̇n Duan Şahbaz, Necla Bi̇rgül İyi̇son

Turkish Journal of Biology

G protein-coupled receptors (GPCRs) are 7-transmembrane proteins that transduce various extracellular signals into intracellular pathways. They are the major target of neuropeptides, which regulate the development, feeding behavior, mating behavior, circadian rhythm, and many other physiological functions of insects. In the present study, we performed RNA sequencing and de novo transcriptome assembly to uncover the GPCRs expressed in the stick insect Carausius morosus. The transcript assemblies were predicted for the presence of 7-transmembrane GPCR domains. As a result, 430 putative GPCR transcripts were obtained and 43 of these revealed full-length sequences with highly significant similarity to known GPCR sequences in …

Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya

Turkish Journal of Biology

Mucolipidosis type II (ML-II, I-cell disease) is a fatal inherited lysosomal storage disease caused by a deficiency of the enzyme N-acetylglucosamine-1-phosphotransferase. A characteristic skeletal phenotype is one of the many clinical manifestations of ML-II. Since the mechanisms underlying these skeletal defects in ML-II are not completely understood, we hypothesized that a defect in osteogenic differentiation of ML-II bone marrow mesenchymal stem cells (BM-MSCs) might be responsible for this skeletal phenotype. Here, we assessed and characterized the cellular phenotype of BM-MSCs from a ML-II patient before (BBMT) and after BM transplantation (ABMT), and we compared the results with BM-MSCs from a …

A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li

Turkish Journal of Biology

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.

Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru

Turkish Journal of Biology

Diclofenac sodium (DS), a potent inhibitor of cyclooxygenase, reduces the release of arachidonic acid and formation of prostaglandins. Being a nonsteroid drug that shows antiinflammatory action, the possible side effects of fetal DS administration gain importance in public and medical applications. Herein, the effects of DS administration (1 mg/kg) during gestational days 5-20 were investigated on the performance of Wistar rat pups in a variety of behavioral tasks. Four-week-old pups were subjected to sensory motor tests, a plus maze, an open field, the Morris water maze, and a radial arm maze. Fetal DS disrupted some sensory motor performances, such as …

A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with …

In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran

Turkish Journal of Biology

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity …

Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz

Turkish Journal of Biology

Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.8 mg/mL) caused dramatic cell death ratios, demonstrated by spot and colony-forming assays, even in autophagy-deficient cells. To unravel the underlying mechanism, …

Identification Of Streptomyces Strains Isolated From Humulus Lupulus Rhizosphere And Determination Of Plant Growth Promotion Potential Of Selected Strains, Fadi̇me Özdemi̇r Koçak

Turkish Journal of Biology

In the present study it was aimed to obtain novel strains of actinomycetes that have the ability to promote plant growth. For this, seven soil samples from the rhizosphere of Humulus lupulus (Pazaryeri, Bilecik) were used and potential isolates were obtained. 16S rRNA genes of 30 isolates were amplified by PCR and sequenced. Eighteen isolates were found to be closely related to Streptomycetes spp. and they were tested for their proteolytic activity, cellulase activity, phosphate solubility, IAA production, biofilm formation, and growth in nitrogen-limited medium. Two isolates, HCI 12 and HCI 36, were selected according to the results of these …

Morten Lauridsen’S Choral Cycle, Nocturnes: A Conductor’S Analysis, Margaret B. Owens

Theses and Dissertations--Music

Morten Lauridsen is one of the most prolific composers of choral music in the 20th and 21st centuries. His characteristic tone is both readily identifiable and timeless. Works such as Lux Aeterna, Les Chansons des Roses, “Sure on this Shining Night” (from Nocturnes) , and “O magnum mysterium” have solidified his place as one of the most important compositional voices in modern choral music.

Lauridsen’s most often-performed choral works have been individual movements excerpted from his larger choral works, due to their accessibility for advanced high school and collegiate choirs. For example, the popular “Dirait-on” comes from the …

Exposing Corruption In Progressive Rock: A Semiotic Analysis Of Gentle Giant’S The Power And The Glory, Robert Jacob Sivy

Theses and Dissertations--Music

English progressive rock band Gentle Giant is catalogued under the progressive (or “prog”) rock genre for a variety reasons, including unique instrumentation, virtuosity, and interesting/unconventional musical attributes. The complexity of their music is often warranted by the sophisticated concepts behind their albums and the deep messages of their songs. The Power and the Glory (TPatG), Gentle Giant’s sixth studio album, is a concept album that emphasizes the rise and corruption of power. What makes their music, especially TPatG, worthy of scholarly attention beyond the simple examination of the compositional techniques employed is the way in which the …

Front Cover, Marine Ornithology

Marine Ornithology

No abstract provided.