Digital Commons Network^™

Developing A Rational Peptide Ligand Design Method Using Cd13 As A Prototype Target Receptor, Md Zahir Uddin

University of the Pacific Theses and Dissertations

Structure based computational peptide design methods have gained significant interest in recent years with the availability of structural insights of protein-protein interactions obtained from the crystal structures. Most of these approaches design new peptide ligands by connecting the crucial amino acid residues from the protein interface and are generally not based on any predicted receptor-ligand interaction. In this work, a peptide design method based on the Knob-Socket model was used to identify the specific ligand residues packing into the receptor interface. This method enables rational peptide ligand design by predicting amino acid residues that will fit best at the binding …

Fliposomes With A Ph-Sensitive Conformational Switch For Anticancer Drug Delivery Against Triple Negative Breast Cancer, Yifan Lu

University of the Pacific Theses and Dissertations

Cancer is the second leading cause of death in the US and worldwide, accounting for 16% of deaths worldwide in 2015. Of more than 100 types of cancers affecting humans, breast cancer is the most common cancer among women and is the second leading cause of death in women. Triple negative breast cancer (TNBC) is a subtype of breast carcinomas defined by the lack of the expression of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2 /neu). The prognosis and survival of TNBC patients remains the poor due to the lack of effective targeted therapy. …

The Relationship Between The Cefi, As A Measure For Executive Function, And The Basc-3, As A Measure Of Externalizing Behavior, On School Problems For Children Receiving Special Education Services, David Looney

University of the Pacific Theses and Dissertations

This study examined the relationship between executive function and externalizing behaviors within a student population that was assessed for special education services. Executive function was measured by using the Comprehensive Executive Function Inventory (CEFI). Externalizing behaviors and school problems was measured using the Behavior Assessment System for Children, Third Edition (BASC-3). Four separate structural equation models were produced and analyzed to examine this relationship. The results from this study indicate that there is a significant direct effect from executive function on externalizing behaviors and school problems, such that higher scores in executive function yielded lower externalizing behavior and school problems …

Transcriptome Analysis For Novel Peptide In Breeding Gland Of Hymenochirus Boettgeri, Minjin Ko

University of the Pacific Theses and Dissertations

The primary goal of the project was to find nucleotide sequences potentially encoding a pheromone from the breeding gland of Hymenochirus boettgeri. The reasons in searching for the sequence of a pheromone were to better understand the organism and to use the information for application in reproduction of other species. Due to climate change and rampant deforestation, such as in Africa’s Congo Basin, many amphibian species are being threatened. With these increasing threats, a viable option for the future may be breeding in captivity for the amphibian species. Pheromone characterization from the breeding gland of Hymenochirus boettgeri may help with …

An Assessment Of Fecal Water Contamination In The Absaroka-Beartooth Wilderness, Daniel Paul Pendergraph 5024527

Graduate Student Theses, Dissertations, & Professional Papers

Wilderness water sources are often defined as pristine or high quality due to the lack of point source pollution. Non-point source pollution from recreation to water resources can be extensive and is well-studied in protected areas globally. Bacterial contamination, specifically fecal bacteria, poses a significant threat to human health because of the risk for outbreaks of illness and disease. Water sources in designated Wilderness areas are particularly vulnerable to fecal water contamination due to high volume of backpackers and lack of backcountry waste facilities. To estimate the occurrence of fecal water contamination in Wilderness water resources, an exploratory analysis was …

Multinucleated Giant Cell Formation And Phenotype, Kevin Lewis Trout

Graduate Student Theses, Dissertations, & Professional Papers

Multinucleated giant cells (MGC) are homotypic macrophage syncytia associated with granulomas. Despite their correlation with pathology, MGC functional contributions to inflammation are relatively unknown. The objective of this work was to gain an understanding of MGC phenotype. First, techniques were developed to better enable the study of these cells in vitro. Second, inorganic particles known to cause inflammation were observed to cause MGC formation in the lungs. Finally, the particle that resulted in the highest macrophage fusion was used together with the in vitro system to compare MGC and macrophage phenotype in response to stimulation. The results contribute to fundamental …

Rescue Of Als Protein Fus Toxicity By Taf, Elliott Hayden

Browse all Theses and Dissertations

Amyotrophic Lateral Scleroses (ALS) is a neurodegenerative disease characterized by the degeneration of upper and lower motor neurons in the brain and spinal cord leading to progressive paralysis and ultimately death within 5 years of symptom onset. Only two drugs are approved by the Food and Drug Administration (FDA) for treating ALS that slow the disease progression by about 3 months. Mutations in the gene Fused in Sarcoma (FUS) cause inherited forms of ALS. FUS is a nuclear, multifunctional RNA-binding protein (RBP) involved in multiple RNA metabolic pathways. Mutations in FUS cause mislocalization of the protein from the nucleus to …

Chondrichthyan Diversity Within The Burlington-Keokuk Fish Bed Of Southeast Iowa And Northwest Illinois (Mississippian: Osagean), Matthew Michael James Hoenig

Browse all Theses and Dissertations

Chondrichthyan remains occur in abundance within a thin layer of limestone at the top of the Burlington Limestone at the point of the contact with the overlying Keokuk Limestone. This layer of rock, the "Burlington-Keokuk Fish Bed," is stratigraphically consistent and laterally extensive in exposures of the Burlington Limestone near its type section along the Iowa-Illinois border. Deposition of the fish bed occurred on the Burlington Continental Shelf carbonate ramp off the subtropical western coast of Laurussia during the Lower Carboniferous (Late Tournaisian; Osagean) due to a drop in sea level, although the specific mechanism(s) that concentrated the vertebrate fossils …

Calpain-Calpastatin System In Peripheral Nerve Myelination And Demyelination, Domenica Elizabeth Drouet Saltos

Browse all Theses and Dissertations

Myelin is the structure formed by glial cells that electrically insulates axons aiding in propagation of action potentials. Disruption of myelin sheath (demyelination) is common to neurological diseases, such as multiple sclerosis (MS). The calcium-dependent protease, calpain, is known to modulate myelination during central nervous system (CNS) development. Endogenous calpain inhibitor, calpastatin, regulates calpain activity. The calpain-calpastatin system has been implicated in the CNS demyelination occurring in diseases. Charcot-Marie-Tooth disease (CMT) is a hereditary demyelinating polyneuropathy affecting proper functioning of peripheral nerves, demonstrating the importance of myelin in the peripheral nervous system (PNS). However, calpain’s role in the mechanism(s) of …

Calcium Imaging Of Developing Proprioceptive Dorsal Root Ganglion Neurons, Kaitlyn Louise Parkes

Browse all Theses and Dissertations

Proprioception is an important sensation capable due to proprioceptive sensory neuron afferents found in muscles in the periphery being processed by the central nervous system. The nervous system is a complex system that continues to develop and mature as an animal ages. Much is not known of proprioceptive neurons and how they develop with time. Calcium is an important molecule in maintaining action potentials and homeostasis in neurons which can be studied to understand a variety of things about a neuron. This study uses genetically encoded calcium indicators to tag parvalbumin positive cells in the dorsal root ganglion (DRG) in …

Enhancing Group Social Perceptiveness Through A Swarm-Based Decision-Making Platform, David A. Askay, Lynn E. Metcalf, Louis Rosenberg, Gregg Willcox

Industrial Technology and Packaging

Swarm Intelligence is natural phenomenon that enables social animals to make group decisions in real-time systems. This process has been deeply studied in fish schools, bird flocks, and bee swarms, where collective intelligence has been observed to emerge. The present paper describes swarm.ai—a collaborative technology that enables swarms of humans to collectively converge upon a decision as a real-time system. Then we present the results of a study investigating if groups working as “human swarms” can amplify their social perceptiveness, a key predictor of collective intelligence. Results showed that groups reduced their social perceptiveness errors by more than half when …

18th And 19th Tshl Legislative Sessions Supporting Documents 2019-2023, Texas Silver Haired Legislature Foundation

Texas Silver-Haired Legislature

Supporting documents from the 18th and 19th Texas Silver-Haired Legislature legislative sessions from 2019-2023.

Investigation Of Iron Mediated Molecular Transformations And Synthesis Of 2-Amino-Α-Carboline And Its Analogues Relevant For Dna Adduct Formation, Anita Adjoa Oppong

Open Access Dissertations

The discovery of new synthetic methods and routes for making new bonds is one of the highest incentives to an organic chemist. Innovative, efficient, effective, economical and exceptionally environmentally friendly synthetic methods are being continuously discovered. Transition metal catalyzed C-H activation has been unearthed as one of these methods, especially iron catalyzed direct C-H activation. Fundamental to making more discoveries is understanding the mechanism behind a specific reaction. To this end, the mechanism behind iron catalyzed C-H activation was investigated. Results indicate the intermediary transition state for the iron species involves an Fe(I) species but the attempt to synthesize this …

Manufacturing Resilience On The Margins: Street Gangs, Property, &Vulnerability Theory, Lua Kamál Yuille

Penn State Law Review (2003 - Present)

Within law, contemporary street gangs are cast as corporatized criminal enterprises, whose primary goal is the acquisition of illicit economic capital. The sophistication of corporate gangs has led to the development of novel control mechanisms like gang injunctions, which are civil legal remedies employed to disperse unwanted gang activity from protected communities. This article suggests that the idea of propertyand the vulnerability associated therewith-is central to understanding gangs. Accepting the well-established proposition that gangs arise due to the unavailability or inaccessibility of markets for mainstream and legitimized forms of capital, this article argues that gangs are best understood as corporate …

Rethinking Pedagogy: Exploring The Potential Of Digital Technology In Achieving Quality Education, Lorenzo Alemán De La Garza, Alessandra Anichini, Péter Antal, Aurélie Beaune, Helen Crompton, Avgoustos Tsinakos, Et Al.

Teaching & Learning Faculty Publications

(First Paragraph) The Mahatma Gandhi Institute of Education for Peace and Sustainable Development (MGIEP) is UNESCO’s Category 1 education Institute in the Asia-Pacific region devoted to education for peace and sustainable development, as enshrined in SDG Target 4.7. UNESCO MGIEP promotes the use of digital learning platforms where teachers and students can co-create and share a highly interactive learning experience. With the rise of the internet, there has been a proliferation of online content and digital resources intended to support teaching and learning, albeit widely varying in quality. Digital education media and resources, if carefully designed and implemented, have a …

Learning With Mobile Devices, Helen Crompton, John Traxler

Teaching & Learning Faculty Publications

The concept of learning with small portable computers was developed by Alan Kay in 1972. Since that early conception, scholars, such as Traxler, Sharples, and Soloway are the pioneering scholars who paved the way to a better understanding of the philosophical, pedagogical, and conceptual underpinnings of mobile learning today. In this chapter, an overview is provided to explicate the initial foundations of the emerging field of mobile learning (mlearning). Next, current scientific knowledge is delineated with explicit references to the early scholars. This chapter concludes with a preview of the future research directions in mobile learning.

Standards Spotlight: Iste Learner Standard Provides A Road Map To Lifelong Scholarship, Helen Crompton

Teaching & Learning Faculty Publications

(First paragraph) Like most educators, I’m a learner first. I want to know all there is to know about teaching to best meet the needs of my students. That’s why I went to college for 15 years to gain my teaching qualifications, including a master’s degree and a Ph.D. in educational technology and mathematics education. I’m now a tenured professor, but I can honestly say that I’m only part way through my learning journey.

Remotely Activated Biomaterials For Optimizing The Temporal Profiles Of Therapeutic Molecules, Tania T. Emi

Open Access Dissertations

Remotely activated hydrogel-based biomaterials can maximize the therapeutic effects by providing localized deliveries and minimizing off-target side effects. Smart hydrogels respond to an externally applied stimulus by delivering therapeutic molecules. While showing promise in releasing molecules in a precise spatial manner, they do not inherently provide temporally dynamic delivery profiles (e.g., controlled timing, rates, and sequences) required to direct sequences of biological events.

Biological processes are characterized by a sequence of events regulated by a complex interplay of signaling factors. For example, in the instance of ischemia (where a tissue no longer receives oxygen and nutrients due to a lack …

Reflections On Changing Pedagogy Of Augmentative And Alternative Communication, Kelly Kleinhans, Lauren E. Bland, Richard Dressler

Journal of Excellence in College Teaching and Learning

Augmentative and alternate communication (AAC) is a service delivery area in the field of speech-language pathology. Individuals with significant and complex communication impairments benefit when a speech-language pathologist develops a communication system that either replaces or augments traditional speech. AAC includes the use of assistive technology designed to represent language and produce voice output. The profession's credentialing body, the American Speech-Language Hearing Association, requires university training programs to document student's knowledge and skills in AAC for working with clients across the lifespan. The authors reflect on how pedagogy associated with AAC has been transformed over time by external influences like …

Reputation Failure: The Limits Of Market Discipline In Consumer Markets, Yonathan A. Arbel

Articles

Many believe that consumer-sourced reputational information about products should increasingly replace top-down regulation. Instead of protecting consumers through coercive laws, reputational information gleaned from the wisdom of the crowd would guide consumer decision-making. There is now a growing pressure to deregulate in diverse fields such as contracts, products liability, consumer protection, and occupational licensing.

This Article presents a common failure mode of systems of reputation: "Reputation Failure." By spotlighting the public-good nature of reviews, rankings, and even gossip, this Article shows the mismatch between the private incentives consumers have to create reputational information and its social value. As a result …

Effect Of Antidepressants On Cytokines Profiles Seen In Inflamed Tissue And Serum Samples In Patients With Moderate Chronic Periodontitis, Silvia M. Villalobos

All ETDs from UAB

This study evaluated whether depression with or without the use of antidepressant was associated with changes in inflammatory markers, intraorally and systemically, GCF volume, serum biomarker levels. Cross-sectional data were collected from individuals with moderate periodontitis who were assigned to one of three groups based upon their medical history, medication usage, and answers to a validated survey instrument that assessed their level of clinical depression. Three groups of patients were compared: 1) patients with clinically diagnosed depression taking antidepressant medications [selective serotonin reuptake inhibitors (SSRIs), norepinephrine-dopamine reuptake inhibitor (NDRIs), or serotonin and norepinephrine reuptake inhibitors (SNRIs)]. 2) patients with clinically …

Modulation Of Klotho Affects Dendritic Spine Remodeling And Neuronal Network Activity, Hai T. Vo

All ETDs from UAB

Klotho protein expression has profound effects on lifespan where klotho-deficient mice exhibit premature aging phenotypes and live only to ~8 weeks of age while klotho-overexpressing mice have lifespans that are at least 20% longer than wild-type mice. Klotho expression also has similar effects on cognitive function as klotho-deficient mice develop cognitive impairment by 7 weeks of age and klotho-overexpressing mice show enhanced cognitive function. These effects also extend to humans as polymorphisms that alter circulating levels of klotho have likewise effects on lifespan and brain function. Despite the fact that modulation of klotho expression has reciprocal effects on cognitive function, …

Discovering The Genetic Architecture Underlying Exercise Response Using Drosophila Melanogaster, Louis P. Watanabe

All ETDs from UAB

The growth of modern jobs, technology, and transportation has coincided with increased sedentary behavior in many developed nations. In the United States, this increase has contributed partially to rising rates of obesity, leading to more than 78 million obese individuals. The annual medical costs associated with obesity are thought to range from 147 to 210 billion dollars. The initial treatment recommendations for individuals with obesity are increased exercise and changes in diet. Despite the strong narrative pushing exercise as a health benefit, individual responses can vary widely, and little is known regarding the genetic networks modulating exercise response. In order …

The Closed Eye As A Model For Dry Eye Disease, Cameron Kenneth Postnikoff

All ETDs from UAB

Every night, during sleep, the tears of the closed eye become significantly more inflammatory, with a notable influx of neutrophils. Preliminary studies suggested that these neutrophils were significantly different than blood-isolated neutrophils, with an increased concentration, upregulated surface expression of inflammatory membrane receptors, but inability to respond to inflammatory stimuli. The purpose of this dissertation was to better understand the contribution of this neutrophil population to ocular surface homeostasis and dry eye disease. Chapter One presents an introduction to the tear film and ocular surface, along with an introduction to dry eye disease and neutrophil biology. Chapter One also discusses …

Transcriptional Dynamics Of Dopaminergic Signaling, Katherine Elizabeth Savell

All ETDs from UAB

Drugs of abuse increase dopamine concentrations in the nucleus accumbens, a key reward structure that integrates contextual and cue-related information and regulates motivated behavior. This surge of dopamine triggers cell signaling cascades that converge in the nucleus to cause changes in gene expression, which are thought to lead to the observed functional and structural alterations in the reward circuit after exposure to drugs of abuse. However, while various drugs of abuse cause transcriptional changes, previously available tools have not had the capacity to deeply characterize these gene programs. Therefore, we optimized a dual lentivirus CRISPR system for targeted gene modulation …

Intracellular Compartment-Specific Abnormalities In Proteasome Expression And Activity In The Superior Temporal Gyrus In Schizophrenia, Madeline R. Scott

All ETDs from UAB

Protein homeostasis is an emerging component of schizophrenia (SZ) pathophysiology. Proteomic alterations in SZ are well-documented; however the underlying mechanism driving these changes remains unknown. The ubiquitin proteasome system (UPS) is fundamental to protein homeostasis regulation, and UPS transcript abnormalities in both blood and brain have been observed in SZ. Supporting a role for UPS dysfunction in SZ, we have reported decreased protein expression of ubiquitin-associated proteins in SZ brain. However, previous work on the proteasome has been limited to transcript expression. In the following studies we sought to address this gap in knowledge by characterizing proteasome dysfunction in the …

Meat: A Novel, Sergey Belyaev, Boris Pilnyak, Ronald D. Leblanc

Faculty Publications

An English-language translation of a Russian novel that appeared in the Soviet thick journal "Novyi Mir" in three installments during February, March, and April of 1936.

A Neonicotinoid Affects The Mating Behavior Of Spalangia Endius (Hymenoptera: Pteromalidae), A Biological Control Agent Of Filth Flies, Bethia H. King, A. N. Kremer

Faculty Articles, Papers, and Other Scholarship

Spalangia endius Walker (Hymenoptera: Pteromalidae) is a parasitoid wasp that is commercially-available as a biological control agent for filth fly pests in livestock-rearing operations. Imidacloprid is often used to control these flies. The present study examined the sublethal effect of field-realistic concentrations of imidacloprid on mating behavior, offspring sex ratios, and male preference for virgin females. After exposure to imidacloprid, S. endius females that survived were less likely to mate than control females, which will result in male-biased sex ratios because only mated females can produce daughters. Males did not avoid exposed females, but exposed females were almost always unreceptive. …

Dissemination Of Imidacloprid Through Dairy Cattle Manure And Its Effect On The Biological Control Agent, Spalangia Endius (Hymenoptera: Pteromalidae), And A Filth Fly Host, Musca Domestica (Diptera: Muscidae), E.R. Burgess, S.M. Watkins, Bethia H. King, K. Chantos-Davidson, A. N. Kremer, J.C. Tournear, J. Morrow, T.J. Hagen, E.R. Gaillard

Faculty Articles, Papers, and Other Scholarship

Filth flies, including house flies, Musca domestica L. (Diptera: Muscidae), develop in animal manure. Adult house flies often are controlled with pesticides such as imidacloprid. How imidacloprid disseminates and persists after it contaminates manure was measured at a dairy farm. A week after application of imidacloprid via fly bait to cattle manure, a mean of approximately 4 ppm of imidacloprid, and as high as 15 ppm, was quantifiable up to 12 cm from the application site, but not farther. Laboratory experiments addressed the impact of 15 ppm of imidacloprid in manure on egg-to-adult development of house flies and on the …

The Development Of A High-Throughput Screening Assay For Acetyl-Coa Carboxylase Using 1,3,6,8-Tetrahydroxynapthalene Synthase, Sarah Elayne Cohen

Honors Capstones

No abstract provided.