Digital Commons Network^™

Hydrogen Peroxide Sensors For Biomedical Applications, Jakob Meier, Eric M. Hofferber, Joseph A. Stapleton, Nicole M. Iverson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Hydrogen peroxide (H2O2) is an important molecule within the human body, but many of its roles in physiology and pathophysiology are not well understood. To better understand the importance of H2O2 in biological systems, it is essential that researchers are able to quantify this reactive species in various settings, including in vitro, ex vivo and in vivo systems. This review covers a broad range of H2O2 sensors that have been used in biological systems, highlighting advancements that have taken place since 2015.

Terpene Synthase Genes Originated From Bacteria Through Horizontal Gene Transfer Contribute To Terpenoid Diversity In Fungi, Qidong Jia, Xinlu Chen, Tobias G. Kollner, Jan Rinkel, Jianyu Fu, Jessy Labbe, Wangdan Xiong, Jeroen S. Dickschat, Jonathan Gershenzon, Feng Chen

Nebraska Center for Biotechnology: Faculty and Staff Publications

Fungi are successful eukaryotes of wide distribution. They are known as rich producers of secondary metabolites, especially terpenoids, which are important for fungi-environment interactions. Horizontal gene transfer (HGT) is an important mechanism contributing to genetic innovation of fungi. However, it remains unclear whether HGT has played a role in creating the enormous chemical diversity of fungal terpenoids. Here we report that fungi have acquired terpene synthase genes (TPSs), which encode pivotal enzymes for terpenoid biosynthesis, from bacteria through HGT. Phylogenetic analysis placed the majority of fungal and bacterial TPS genes from diverse taxa into two clades, indicating ancient …

Contemporary Sexual Selection Does Not Explain Variation In Male Display Traits Among Populations, J. Colton Watts, Allissa Flynn, Brigitte Tenhumberg, Eileen Hebets

Brigitte Tenhumberg Papers

Sexual selection is widely hypothesized to facilitate the evolution of reproductive isolation through divergence in sexual traits and sexual trait preferences among populations. However, direct evidence of divergent sexual selection causing intraspecific trait divergence remains limited. Using the wolf spider Schizocosa crassipes, we characterized patterns of female mate choice within and among geographic locations and related those patterns to geographic variation in male display traits to test whether divergent sexual selection caused by mate choice explains intraspecific trait variation. We found evidence of phenotypic selection on male behavior arising from female mate choice, but no evidence that selection varied …

Is There A Correlation Between Oral Reading Rate And Social Conversational Speaking Rate?, Sarah C. Mason

Graduate Research Papers

The purpose of this research was to determine if there was a correlation between oral reading rates and social conversational speaking rates. This research was conducted using a sample size of 25 students, ages 8-12, in one elementary building in Southern Iowa. Student data were collected from a previous district’s fall assessment in the area of oral reading rate. Students were given specific prompts when providing their social conversational speaking. When comparing oral reading rate with their social conversational speaking rate, it was found that there was a correlation when measured in words per minute suggesting that students’ reading rates …

The Effect Of Digital Game-Based Learning On Student Learning: A Literature Review, Katrina Serrano

Graduate Research Papers

Digital game-based learning can be used by educators to support students in developing skills. This review examined the effect of digital game-based learning on student learning at the K-12 level. Sixteen peer-reviewed research studies, two meta-analysis studies, and two literature reviews published between 2011-2019 were selected for analysis. The reviewed research indicated that when digital game-based learning is used that includes key game design elements (collaboration, choice, feedback) as well as instructional design, there typically is a positive impact on student engagement. Research also indicated that digital game-based learning along with collaboration can have a significant effect on student motivation, …

Kids Say The Darndest Things: Minors And The Internet, Emily Diroma

Cardozo Law Review de•novo

This Note argues that due to the inherent failures of COPPA and the ever rising online presence of both teenagers and minors, the FTC should expand COPPA to include eraser and advertisement protection provisions similar to those found in SB 568 in order to further ensure the protection teenagers and minors online.

Mapping Of Lymphatic Filariasis In Loiasis Areas: A New Strategy Shows No Evidence For Wuchereria Bancrofti Endemicity In Cameroon, Samuel Wanji, Mathias Eyong Esum, Abdel Jelil Njouendou, Amuam Andrew Mbeng, Patrick W Chounna Ndongmo, Raphael Awah Abong, Jerome Fru, Fanny F. Fombad, Gordon Takop Nchanji, Glory Ngongeh, Narcisse V. Ngandjui, Peter Ivo Enyong, Helen Storey, Kurt C. Curtis, Kerstin Fischer, Joseph R. Fauver, Daphne Lew, Charles W. Goss, Peter U. Fischer

Journal Articles: Epidemiology

BACKGROUND: Mapping of lymphatic filariasis (LF) caused by Wuchereria bancrofti largely relies on the detection of circulating antigen using ICT cards. Several studies have recently shown that this test can be cross-reactive with sera of subjects heavily infected with Loa loa and thus mapping results in loiasis endemic areas may be inaccurate.

METHODOLOGY/PRINCIPAL FINDINGS: In order to develop an LF mapping strategy for areas with high loiasis prevalence, we collected day blood samples from 5,001 subjects residing in 50 villages that make up 6 health districts throughout Cameroon. Antigen testing using Filarial Test Strip (FTS, a novel platform that uses …

Criar Y Dejarse Criar: Trans-Situ Crop Conservation And Indigenous Landscape Management Through A Network Of Global Food Neighborhoods, Cass Madden

Capstone Collection

As climate change progresses, global food security is likely to become increasingly threatened and crop biodiversity will be a significant source of resiliency and adaptability. However, these adaptations will only be fully realized through cooperative in situ and ex situ conservation and cultivation of domesticated crops, crop wild relatives, and wild foods. This conservation is best realized in places where communities have the cultural resources to invest meaningfully in the cultivation of native crops, and where the cultivation of those crops can reinforce place-specific livelihoods and identities. To this end, the principal objective of this research is to propose a …

Review Of Soil Salinity Assessment For Agriculture Across Multiple Scales Using Proximal And/Or Remote Sensors, Dennis Corwin, E. Scudiero

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mapping and monitoring soil spatial variability is particularly problematic for temporally and spatially dynamic properties such as soil salinity. The tools necessary to address this classic problem only reached maturity within the past 2 decades to enable field- to regional-scale salinity assessment of the root zone, including GPS, GIS, geophysical techniques involving proximal and remote sensors, and a greater understanding of apparent soil electrical conductivity (ECa) and multi- and hyperspectral imagery. The concurrent development and application of these tools have made it possible to map soil salinity across multiple scales, which back in the 1980s was prohibitively expensive and impractical …

Improving Socioeconomic Status May Reduce The Burden Of Malaria In Sub Saharan Africa: A Systematic Review And Meta-Analysis, Abraham Degarege, Kristopher Fennie, Dawit Degarege, Shasank Chennupati, Purnima Madhivanan

Journal Articles: Epidemiology

BACKGROUND: A clear understanding of the effects of housing structure, education, occupation, income, and wealth on malaria can help to better design socioeconomic interventions to control the disease. This literature review summarizes the relationship of housing structure, educational level, occupation, income, and wealth with the epidemiology of malaria in sub-Saharan Africa (SSA).

METHODS: A systematic review and meta-analysis was conducted following the preferred reporting items for systematic reviews and meta-analyses guidelines. The protocol for this study is registered in PROSPERO (ID=CRD42017056070), an international database of prospectively registered systematic reviews. On January 16, 2016, available literature was searched in PubMed, Embase, …

Impact Of Cognitive Distortions And Perceived Stigmatization On Acceptance Among Adults With Neurofibromatosis Type 1, Shanna C. Drinkwine

PCOM Psychology Dissertations

Neurofibromatosis type 1 (NF1) is a genetic condition that is characterized by many physical and cognitive signs and symptoms. Many of the physical manifestations are widespread and visible, which may cause an individual to be a target of stigmatization. An individual’s perception of this stigmatization could lead to emotional distress and a decreased quality of life. To date, there is no research investigating the underlying cognitive factors that drive emotional responses to these negative events in individuals with NF1. More specifically, a literature review reveals no research indicating that cognitive distortions or acceptance have ever been studied in this population. …

Drug Prices, Dying Patients, And The Pharmaceutical Marketplace: A New Conditional Approval Pathway For Critical Unmet Medical Needs, Robert A. Bohrer

Faculty Scholarship

Prescription drugs have been a major topic in the news for much of the past year. There are two issues which appear often: first, the very high prices of new drugs, particularly the "specialty" drugs developed for serious diseases; and second, the time required for FDA approval in relation to the perceived need for earlier access to new therapies for critically ill patients. Much less in the news, but lurking behind both issues, is the need for better information for physicians and patients to use in making decisions about prescribing and taking drugs, and for insurance companies and the government …

Direct-To-Consumer Ads Are Misleading: Concise Statements Of Effectiveness Should Be Required, Robert A. Bohrer

Faculty Scholarship

Direct-to-consumer (DTC) advertising of prescription pharmaceuticals has been the subject of much criticism and the issue has become even more pressing with the Trump administration’s proposal to require the disclosure of prices in DTC ads. In this article I argue that a more powerful approach to the problem of DTC ads would require the disclosure of the effectiveness of the advertised drugs, at least as found in the clinical trials submitted for FDA approval. To support the need for an effectiveness disclosure, I describe the problem of DTC ads and examine representative ads to illustrate the potential of such ads …

Dicer Regulates Activation Of The Nlrp3 Inflammasome, David M. Ojcius, Ardavan Jafari, Laxmi Yeruva, Christian W. Schindler, Ali A. Abdul-Sater

All Dugoni School of Dentistry Faculty Articles

Inflammation plays a critical role in initiation of adaptive immunity, pathogen clearance and tissue repair. Interleukin (IL)-1β is a potent pro-inflammatory cytokine and therefore its production is tightly regulated: its secretion requires the assembly of a macromolecular protein complex, termed the inflammasome. Aberrant activation of the inflammasome has been linked to debilitating human diseases including chronic inflammatory and autoimmune diseases. Thus, there is a great interest in understanding how inflammasomes are regulated. Here we show that Dicer, an enzyme necessary for the production of mature micro-RNAs (miRNAs), is required for optimal activation of NLRP3 inflammasomes in bone marrow macrophages. Our …

Automation And Predictive Analytics In Patent Prosecution: Uspto Implications And Policy, Tabrez Y. Ebrahim

Faculty Scholarship

Artificial-intelligence technological advancements bring automation and predictive analytics into patent prosecution. The information asymmetry between inventors and patent examiners is expanded by artificial intelligence, which transforms the inventor-examiner interaction to machine-human interactions. In response to automated patent drafting, automated office-action responses, "cloems" (computer-generated word permutations) for defensive patenting, and machine-learning guidance (based on constantly updated patent-prosecution big data), the United States Patent and Trademark Office (USPTO) should reevaluate patent-examination policy from economic, fairness, time, and transparency perspectives. By conceptualizing the inventor-examiner relationship as a "patenting market," economic principles suggest stronger efficiencies if both inventors and the USPTO have better information …

Data-Centric Technologies: Patent And Copyright Doctrinal Disruptions, Tabrez Y. Ebrahim

Faculty Scholarship

Data-centric technologies create information content that directly controls, modifies, or responds to the physical world. This information content resides in the digital world yet has profound economic and societal impact in the physical world. 3D printing and artificial intelligence are examples of data-centric technologies. 3D printing utilizes digital data for eventual printing of physical goods. Artificial intelligence learns from data sets to make predictions or automated decisions for use in physical applications and systems. 3D printing and artificial intelligence technologies are based on digital foundations, blur the digital and physical divide, and dramatically improve physical goods, objects, products, or systems. …

Influence Of Implicit-Bias Training On The Cultural Competency Of Police Officers, Marvin Whitfield

Walden Dissertations and Doctoral Studies

Highly publicized media events involving African American men and the use of deadly force by police officers have occurred between 2013 and 2014. These events have emphasized the need to examine the influence of implicit bias training on police officers' decision-making processes. During the past two decades, Community Oriented Policing Services has invested several billion dollars in training programs designed to eliminate racial bias within the law enforcement community. The purpose of this qualitative study was to examine how implicit-bias training influences the decision-making processes of police officers. More specifically, this study examined the perceptions of police officers regarding the …

Some Current Trends And Challenges In Philippine Aquaculture, With An Emphasis On Synergies With Biodiversity Initiatives, Ronald Allan L. Cruz, Vikas Kumar, Janice A. Ragaza

Biology Faculty Publications

No abstract provided.

A Rapid And Accurate Minion-Based Workflow For Tracking Species Biodiversity In The Field, Hendrik Freitag, Simone Maestri, Emanuela Cosentino, Marta Paterno, Jhoana M. Garces, Luca Marcolungo, Massimiliano Alfano, Iva Njunjić, Menno Schilthuizen, Ferry Slik, Michele Menegon, Marzia Rossato, Massimo Delledonne

Biology Faculty Publications

Genetic markers (DNA barcodes) are often used to support and confirm species identification. Barcode sequences can be generated in the field using portable systems based on the Oxford Nanopore Technologies (ONT) MinION sequencer. However, to achieve a broader application, current proof-of-principle workflows for on-site barcoding analysis must be standardized to ensure a reliable and robust performance under suboptimal field conditions without increasing costs. Here, we demonstrate the implementation of a new on-site workflow for DNA extraction, PCR-based barcoding, and the generation of consensus sequences. The portable laboratory features inexpensive instruments that can be carried as hand luggage and uses standard …

Poly-Arginine Peptide R18d Reduces Neuroinflammation And Functional Deficits Following Traumatic Brain Injury In The Long-Evans Rat, Li Shan Chiu, Ryan S. Anderton, Jane L. Cross, Vince W. Clark, Neville W. Knuckey, Bruno P. Meloni

Health Sciences Papers and Journal Articles

We have previously demonstrated that the poly-arginine peptide R18 can improve histological and functional outcomes following traumatic brain injury (TBI) in the Sprague–Dawley rat. Since D-enantiomer peptides are often exploited in pharmacology for their increased stability and potency, the present study compared the effects of R18 and its D-enantiomer, R18D, following TBI in the Long-Evans rat. Following a closed-head impact delivered via a weight-drop apparatus, peptide was administered at a dose of 1000 nmol/kg at 30 min after TBI. Treatment with R18D, but not R18 resulted in significant reductions in sensorimotor (p = 0.026) and vestibulomotor (p = 0.049) deficits …

Metabolic Effects Of 17a-Estradiol Are Growth Hormone Independent And Sex Specific, Silvana Sidhom

Electronic Theses and Dissertations

Aging is a major risk factor for metabolic syndromes and type two diabetes. With growing elderly populations worldwide and increasing incidence of age-related diseases there is a great need to develop pharmacological interventions that would delay aging and protect from age-related diseases. 17-alpha estradiol (17α-E2) is an epimer of the primary female sex hormone estradiol and has been shown to extend lifespan and downregulate markers of age-related metabolic dysfunction in male mice. Because 17α-E2 does not induce feminization in males it holds potential as a novel therapeutic in humans for age-related metabolic dysfunction. Importantly, we have previously shown that 17α-E2 …

Identification And Functional Characterization Of A Long Non-Coding Rna Associated With Prostate Cancer, Md Faqrul Hasan

Electronic Theses and Dissertations

Prostate cancer is the most common cancer in men in the western world. Although early stage prostate cancer is treatable late stage, more specifically, metastatic and drug resistant prostate cancers are mostly incurable. The failure of current treatments obligates the research community to explore novel areas in prostate cancer biology and find better therapeutic targets. Emerging evidences show that non-coding RNAs specifically long non-coding RNAs (lncRNAs) play regulatory roles in various cellular processes and are frequently dysregulated in cancer including prostate cancer. These aberrantly expressed lncRNAs mostly with unexplored genetic information may drive cancer progression. Previous studies done in our …

Development Of A Functional Testing Platform For The Sensory Segment Of The Neuromuscular Reflex Arc, Alisha Colon

Electronic Theses and Dissertations

Investigations of human biology and disease have been hindered by the use of animal models. The information obtained from such studies often results in clinically irrelevant results and drug trial failures. Additionally, several governing bodies have been formulating legislation to move away from animal models and toward more ethical and efficient testing platforms for drug discovery and cosmetic research. As an answer to these issues, "body-on-a-chip" systems have been a rapidly developing field which easily recapitulates in vivo functionality, providing a more relevant, repeatable, and ethical testing platform to better predict biology. These systems can be used as human-based testing …

Human-Wildlife Conflict And Coexistence In A More-Than-Human World: A Multiple Case Study Exploring The Human-Elephant-Conservation Nexus In Namibia And Sri Lanka, Cynthia Castaldo-Walsh

Department of Conflict Resolution Studies Theses and Dissertations

This qualitative multiple case study explored human-elephant conflict-coexistence relationships and issues of conservation in Namibia (Damaraland) and Sri Lanka (Wasgamuwa) from a posthumanist, multispecies perspective. Within each region, conflict between humans and elephants is considered high, elephants are considered endangered and are of high conservation priority, the human population has grown significantly, and community-based organizations are implementing holistic approaches to increase positive relations between humans and elephants. This study was guided by research questions that explored the current landscape of the human-elephant-conservation nexus within each region, the shared histories between humans and elephants over time, and the value in utilizing …

Post-Decisional Conflict In Selecting Cancer Treatments: Perception Of Information Disclosure May Influence Decisional Conflict, Decisional Regret, And Self-Acceptance In Bereaved Parents Of Children With Cancer, Danielle De Santis Sperandeo

Department of Conflict Resolution Studies Theses and Dissertations

This study aimed to establish a connection, if any, between perceptions of information disclosure about medical treatment and decisional conflict in bereaved parents of children with cancer. Decisional regret was an important theme in this exploration because decisional conflict strongly aligns with the propensity to mentally redo past events, thereby forming counterfactual alternatives to reality. People generate counterfactuals to hypothesize a more favorable outcome subsequent to a negative event or the death of a child as applicable to this study. A secondary objective was to investigate the potential influence of counterfactual processing and regret on the construct of self-acceptance: a …

The Political Animal: The Animal Rights Movement And Public Policy, Dorothy Jean Simnett

Department of Conflict Resolution Studies Theses and Dissertations

Social movements are consequences of social conflict. This project examines the conflicting and contentious efforts of animal rights activists at changing public policy and it evaluates the successes and failures of the animal rights movement. Animal rights activists’ challenges concerning animal rights, animal welfare and animal protection, combined with divergences from the special interest consortiums compounded by societal norms will all be addressed here. Furthermore, none of this has been previously addressed in the field of Conflict Resolution. This project highlights the challenges that animal rights advocates encounter when attempting to safeguard animal protective measures. The power struggle between animal …

Critics On Critics: Queer Bonds, Chase Gregory

Faculty Journal Articles

No abstract provided.

Complexity And Conflict: The Interplay Of Policy, Perception And Language In Post Ada America, Sheila Northrop

Department of Conflict Resolution Studies Theses and Dissertations

The purpose of the dissertation is to examine the language used to discuss disability and the attitudes and perceptions that the general American public has about people living with disabilities. Using the transformative research and evaluation method, the content analysis study examines the use of “deficit perspective language” versus “non-deficit perspective language” as defined by Mertens in materials available to the general American public that address the five areas described in the World Health Organization matrix on community rehabilitation for people with disabilities. The mixed methods study explores both the frequency of deficit perspective language and the frequency non- deficit …

Divergent Antigen-Specific Cellular Immune Responses During Asymptomatic Subclinical And Clinical States Of Disease In Cows Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Judith R. Stabel, J. P. Bannantine

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Infection of the host with Mycobacterium avium subsp. paratuberculosis results in chronic and progressive enteritis that traverses both subclinical and clinical stages. The mechanism(s) for the shift from an asymptomatic subclinical disease state to advanced clinical disease is not fully understood. In the present study, naturally infected dairy cattle were divided into subclinical and clinical infection groups, along with noninfected control cows of similar parity, to study host immune responses in different stages of infection. Both infection groups had higher levels of secretion of gamma interferon (IFN-􏰇), tumor necrosis factor-alpha (TNF-􏰈), and interleukin-2 (IL-2) than control cows, whereas only clinical …

Private Ordering And Commercial Arbitration: Lasting Lessons From Mentschikoff, Stephen J. Ware

Journal of Dispute Resolution

No abstract provided.