Digital Commons Network^™

Efficacy Of Prosopilosidine From Prosopis Glandulosa Var. Glandulosa Against Cryptococcus Neoformans Infection In A Murine Model, Mohammad Ashfaq, Mohamed Sadek Abdel-Bakky, Mir Tahir Maqbool, Volodymyr Samoylenko, Aziz Abdur Rahman, Ilias Muhammad

Faculty and Student Publications (Open Access)

In this study, 2,3-dihydro-1H-indolizinium alkaloid-prosopilosidine (PPD), that was isolated from Prosopis glandulosa, was evaluated against C. neoformans in a murine model of cryptococcosis. In vitro and in vivo toxicity of indolizidines were also evaluated. Mice were infected via the tail vein with live C. neoformans. Twenty-four hours post-infection, the mice were treated with PPD once a day (i.p.) or twice a day (bid) orally, or with amphotericin B (Amp B) intraperitoneally (IP), or with fluconazole (Flu) orally for 5 days. The brains of all of the animals were aseptically removed and the numbers of live C. neoformans were recovered. In …

Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline Alexandria De Lora

Biomedical Sciences ETDs

Adaptation of cancer cells to changes in the biochemical microenvironment in an expanding tumor mass is a crucial aspect of malignant progression, tumor metabolism, and drug efficacy. In vitro, it is challenging to mimic the evolution of biochemical gradients and the cellular heterogeneity that characterizes cancer tissues found in vivo. It is well accepted that more realistic and controllable in vitro 3D model systems are required to improve the overall cancer research paradigm and thus improve on the translation of results, but multidisciplinary approaches are needed for these advances. This work develops such approaches and demonstrates that new droplet-based cell-encapsulation …

Modeling Semantic Encoding In A Common Neural Representational Space, Cara E. Van Uden, Samuel A. Nastase, Andrew C. Connolly, Ma Feilong, Isabella Hansen, M Ida Gobbini, James V. Haxby

Dartmouth Scholarship

Encoding models for mapping voxelwise semantic tuning are typically estimated separately for each individual, limiting their generalizability. In the current report, we develop a method for estimating semantic encoding models that generalize across individuals. Functional MRI was used to measure brain responses while participants freely viewed a naturalistic audiovisual movie. Word embeddings capturing agent-, action-, object-, and scene-related semantic content were assigned to each imaging volume based on an annotation of the film. We constructed both conventional within-subject semantic encoding models and between-subject models where the model was trained on a subset of participants and validated on a left-out participant. …

Metabolic Acidosis After Sodium Thiosulfate Infusion And The Role Of Hydrogen Sulfide, Guenola M. Hunt, Hilary F. Ryder

Dartmouth Scholarship

Sodium thiosulfate (STS), first-line treatment for calcific uremic arteriolopathy, causes a mild asymptomatic acidosis in many patients. However, severe, life-threatening acidosis out of proportion with the expected acid load of STS may occur, potentially due to metabolism of STS to hydrogen sulfide.

Influence Of Behavioral State, Sex, And Season On Resource Selection By Jaguars (Panthera Onca): Always On The Prowl?, Eric M. Gese, Patricia A. Terletzky, Sandra M. C. Calvalcanti, Christopher M. U. Neale

Wildland Resources Faculty Publications

How a predator uses its landscape to move through its territory and acquire prey is a fundamental question for scientific research. The influence of abiotic and biotic factors on space use of large carnivores has profound implications for their future management and conservation. In the Pantanal, Brazil, jaguars (Panthera onca) are the apex predator, but conflicts with cattle depredations pose a risk to their future conservation. We examined whether behavioral state, sex, and season influenced how jaguars used the landscape in the Pantanal. To accomplish this, we radio‐collared four females and six males; radio‐collared jaguars were monitored for …

Rapid Screening Of Ellagitannins In Natural Sources Via Targeted Reporter Ion Triggered Tandem Mass Spectrometry, Jeremiah J. Bowers, Harsha P. Gunawardena, Anaëlle Cornu, Ashwini S. Narvekar, Antoine Richieu, Denis Deffieux, Stéphane Quideau, Nishanth Tharayil

Publications

Complex biomolecules present in their natural sources have been difficult to analyze using traditional analytical approaches. Ultrahigh-performance liquid chromatography (UHPLC-MS/MS) methods have the potential to enhance the discovery of a less well characterized and challenging class of biomolecules in plants, the ellagitannins. We present an approach that allows for the screening of ellagitannins by employing higher energy collision dissociation (HCD) to generate reporter ions for classification and collision-induced dissociation (CID) to generate unique fragmentation spectra for isomeric variants of previously unreported species. Ellagitannin anions efficiently form three characteristic reporter ions after HCD fragmentation that allows for the classification of unknown …

Aerosol Microdroplets Exhibit A Stable Ph Gradient, Haoran Wei, Eric P. Vejerano, Weinan Leng, Qishen Haung, Marjorie R. Willner, Linsey C. Marr, Peter J. Vikesland

Faculty Publications

Suspended aqueous aerosol droplets (<50 μm) are microreactors for many important atmospheric reactions. In droplets and other aquatic environments, pH is arguably the key parameter dictating chemical and biological processes. The nature of the droplet air/ water interface has the potential to significantly alter droplet pH relative to bulk water. Historically, it has been challenging to measure the pH of individual droplets because of their inaccessibility to conventional pH probes. In this study, we scanned droplets containing 4-mercaptobenzoic acid–functionalized gold nanoparticle pH nanoprobes by 2D and 3D laser confocal Raman microscopy. Using surface-enhanced Raman scattering, we acquired the pH distribution inside approximately 20-μm-diameter phosphate-buffered aerosol droplets and found that the pH in the core of a droplet is higher than that of bulk solution by up to 3.6 pH units. This finding suggests the accumulation of protons at the air/water interface and is consistent with recent thermodynamic model results. The existence of this pH shift was corroborated by the observation that a catalytic reaction that occurs only under basic conditions (i.e., dimerization of 4-aminothiophenol to produce dimercaptoazobenzene) occurs within the high pH core of a droplet, but not in bulk solution. Our nanoparticle probe enables pH quantification through the cross-section of an aerosol droplet, revealing a spatial gradient that has implications for acid-base–catalyzed atmospheric chemistry.

Down Regulation Of Vestibular Balance Stabilizing Mechanisms To Enable Transition Between Motor States, Romain Tisserand, Chris J. Dakin, Machiel H. F. Van Der Loos, Elizabeth A. Croft, Timothy J. Inglis, Jean-Sebastien Blouin

Kinesiology and Health Science Faculty Publications

The neural control of transition between posture and movement encompasses the regulation of reflex-stabilizing mechanisms to enable motion. Optimal feedback theory suggests that such transitions require the disengagement of one motor control policy before the implementation of another. To test this possibility, we investigated the continuity of the vestibular control of balance during transitions between quiet standing and locomotion and between two standing postures. Healthy subjects initiated and terminated locomotion or shifted the distribution of their weight between their feet, while exposed to electrical vestibular stimuli (EVS). The relationship between EVS and ground reaction forces was quantified using time-frequency analyses. …

Comparative Strengths Of Tetrel, Pnicogen, Chalcogen, And Halogen Bonds And Contributing Factors, Wenbo Dong, Qingzhong Li, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

Ab initio calculations are employed to assess the relative strengths of various noncovalent bonds. Tetrel, pnicogen, chalcogen, and halogen atoms are represented by third-row atoms Ge, As, Se, and Br, respectively. Each atom was placed in a series of molecular bonding situations, beginning with all H atoms, then progressing to methyl substitutions, and F substituents placed in various locations around the central atom. Each Lewis acid was allowed to engage in a complex with NH3 as a common nucleophile, and the strength and other aspects of the dimer were assessed. In the context of fully hydrogenated acids, the strengths of …

Radio-Fluorogenic Gel Dosimetry With Coumarin., Peter A Sandwall, Brandt P Bastow, Henry B Spitz, Howard R Elson, Michael Lamba, William B Connick, Henry Fenichel

Oncology Articles

Gel dosimeters are attractive detectors for radiation therapy, with properties similar to biological tissue and the potential to visualize volumetric dose distributions. Radio-fluorogenesis is the yield of fluorescent chemical products in response to energy deposition from ionizing radiation. This report shares the development of a novel radio-fluorogenic gel (RFG) dosimeter, gelatin infused with coumarin-3-carboxlyic acid (C3CA), for the quantification of imparted energy. Aqueous solutions exposed to ionizing radiation result in the production of hydroxyl free radicals through water radiolysis. Interactions between hydroxyl free radicals and coumarin-3-carboxylic acid produce a fluorescent product. 7-hydroxy-coumarin-3-carboxylic acid has a blue (445 nm) emission following …

Perspectives Of Older Blacks And Whites Living With Serious Mental Illness About Outpatient Mental Health Services, Rosalyn Roker

USF Tampa Graduate Theses and Dissertations

In the United States, over three million adults, age 50 and older, reported a diagnosis of serious mental illness (SMI) in the past year. Most of them live in community-settings and are less likely than younger adults to utilize mental health treatment. Lack of and insufficient treatment for SMI places them at increased risk of morbidity, earlier mortality, cognitive decline, and diminished quality of life. The current study aimed to: (1) examine the factors that influence Black and White older adults, who live with SMI, to seek and engage in outpatient mental health treatment; (2) identify the perspectives of Black …

Catalytic Functionalization Of Organoaluminum Reagents And Olefins By Cross-Coupling With Copper And Nickel, Bijay Shrestha

Chemistry and Chemical Biology ETDs

This thesis is divided into two parts. The first part focuses on the Cu-catalyzed cross-coupling of organoaluminum reagents with organohalides. One of the most powerful tools for the construction of C-C bonds is cross-coupling. But, this reaction is predominantly catalyzed by Pd, a rare and expensive transition metal, which inevitably makes the process unsustainable in the long-term. Furthermore, Pd-catalyzed cross-coupling also shows low tolerance for alkyl and heteroaryl substrates because of β-hydride elimination and catalyst deactivation, respectively. Although these issues remain largely solved by using sterically hindered and electron-rich ligands, however making these types of ligands is synthetically challenging and …

Assessing Subject Areas Of Worldwide Information Literacy Research And Practice: A Discipline Co-Occurrence Network Analysis Approach, Punit Kumar Singh, Prof. Ajay P. Singh

Library Philosophy and Practice (e-journal)

IL is important due to its potentiality to optimize the use of available information and to transform the novice into self-directed lifelong learners. It has gained ground and much attention in every field of knowledge which is assured by rapid increase in related literature. Since, the IL skills require subject-oriented approach not only to develop standard, guide, framework, tools, etc. but also to evaluate, assess, and impact of IL skills. Thus, measuring of the subject areas of IL publications and it co-occurrence is imperative and the objective of the present study. Based on data from Scopus database, network visualization technique …

Abdomen Actin’ Up: A Unique Presentation Of Disseminated Abdominal Actinomycosis, Arpana Parachuri, Md, Brianna Shinn, Md, Rino Sato, Md

The Medicine Forum

INTRODUCTION

Abdominal actinomycosis is a chronic, indolent disease characterized by nonspecific symptoms such as fatigue, weight loss, fever, and abdominal pain. Actinomyces is a genus of fastidious, gram-positive, non-acid-fast, branching filamentous bacilli characterized by sulfur granules that is normally found in oral flora and inhabits the gastrointestinal (GI) tract. Actinomyces infections are relatively rare, however when present, they have the ability to invade multiple organs and disseminate throughout multiple body cavities. Factors that increase the risk of developing actinomycosis include poor oral hygiene, alcoholism, and preexisting dental disease. Intrauterine devices (IUDs) also increase the risk of developing pelvic actinomycosis. Over …

How Does Training From A Stem Elementary Education Program Influence An Elementary Teacher’S Instruction And Experiences?, Courtney G. Mayakis, Jessica R. Robinson, John Williams Iii

Journal of STEM Teacher Education

In the United States, innovation and our economy seem to be lacking in comparison to other countries. Many cite the shortage of individuals interested in STEM careers as part of the problem. The following research article addresses how STEM programs may influence the efficacy and practice of curriculum integration as well as the mathematics and science instruction of teachers in elementary education. Data were collected using interviews and taped instruction from an elementary educator who graduated from a STEM-focused elementary program. This exploratory case study will aid in understanding how preservice programs focusing on STEM-integrated curriculum in elementary education can …

Parts Of The Whole: Institutional Research, Service-Learning, And Nnn, Dorothy Wallace

Numeracy

This essay outlines how faculty interested in service learning could be helpful to an organization such as the National Numeracy Network by conducting institutional research. As a preliminary example, students in a statistics class used online research to determine the percent of colleges and universities with a quantitative reasoning center or with quantitative reasoning courses within mathematics departments. The students found that 46% of two-year colleges and 37% of four-year colleges offer a quantitative reasoning course through the mathematics department, usually for credit but not always in response to an institutional requirement. Only 19% of two-year colleges and 25% of …

The Connection Between Financial Literacy And Numeracy: A Case Study From India, J.D. Jayaraman, Saigeetha Jambunathan, Kenneth Counselman

Numeracy

Making financial decisions involves mathematical calculations, both simple and complex. It is a well-documented fact that financial literacy levels among young people all over the world are quite low and that these low levels contribute to various undesirable outcomes with respect to personal financial well-being and the economy as a whole. This study explores the relationship between financial literacy and numeracy by measuring and modeling the relationship between financial literacy and numeracy levels among high school students (N = 586) in India. The results show a strong relationship between numeracy and financial literacy skills. Low numeracy is associated with …

The Silent Weapons, H. C. Atwood Jr., Robin Clarke

Naval War College Review

No abstract provided.

Presence/Absence And Density Data For Epipelagic Tows Collected During R/V Blazing Seven Cruises Lf2016a And Lf2016b, Northern Gulf Of Mexico From 2016-06-09 To 2016-07-28, Jay R. Rooker, David Wells

DEEPEND Datasets

This dataset reports presence/absence and density data for epipelagic tows collected in the northern Gulf of Mexico during R/V Blazing Seven cruises LF2016A and LF2016B (2016-06-09 to 2016-07-28). Larval fishes were sampled from 48 stations and cruise data were collected at each site including latitude/longitude, date, time, environmental data (temperature, salinity, dissolved oxygen) and Sargassum dry weight. Larval catch data before and after the oil spill will be compared to improve our understanding of the causes of temporal variability as it relates to the Deep-Water Horizon oil spill (DWHOS). Habitat associations of selected taxa (billfishes, tunas, dolphinfishes, flyingfishes) will be …

Results From A 78-Week, Single-Arm, Open-Label Phase 2 Study To Evaluate Ux007 In Pediatric And Adult Patients With Severe Long-Chain Fatty Acid Oxidation Disorders (Lc-Faod)., Jerry Vockley, Barbara Burton, Gerard T Berry, Nicola Longo, John Phillips, Pranoot Tanpaiboon, +Several Additional Authors

Pediatrics Faculty Publications

Long-chain fatty acid oxidation disorders (LC-FAOD) are rare disorders characterized by acute crises of energy metabolism and severe energy deficiency that may present with cardiomyopathy, hypoglycemia, and/or rhabdomyolysis, which can lead to frequent hospitalizations and early death. An open-label Phase 2 study evaluated the efficacy of UX007, an investigational odd-carbon medium-chain triglyceride, in 29 subjects with severe LC-FAOD. UX007 was administered over 78 weeks at a target dose of 25-35% total daily caloric intake (mean 27.5%). The frequency and duration of major clinical events (hospitalizations, emergency room visits, and emergency home interventions due to rhabdomyolysis, hypoglycemia, and cardiomyopathy) occurring during …

Temporal Transcriptome Profiling Of Developing Seeds Reveals A Concerted Gene Regulation In Relation To Oil Accumulation In Pongamia (Millettia Pinnata), Jianzi Huang, Xuehong Hao, Ye Jin, Xiaohuan Guo, Qing Shao, Kavitha Kumar, Yogesh Ahlawat, David Harry, Chandrashekhar Joshi, Yizhi Zheng

Department of Biological Sciences Publications

Background

Pongamia (Millettia pinnata syn. Pongamia pinnata), an oilseed legume species, is emerging as potential feedstock for sustainable biodiesel production. Breeding Pongamia for favorable traits in commercial application will rely on a comprehensive understanding of molecular mechanism regulating oil accumulation during its seed development. To date, only limited genomic or transcript sequences are available for Pongamia, while a temporal transcriptome profiling of developing seeds is still lacking in this species.

Results

In this work, we conducted a time-series analysis of morphological and physiological characters, oil contents and compositions, as well as global gene expression profiles in developing Pongamia …

Intuition In Employee Selection: Examining The Conditions For Accurate Intuitive Hiring Decisions, Vinod Vincent

Doctor of Business Administration Dissertations

In complex organizational environments, managers often rely on intuition to make decisions. Research has found intuition to be helpful when the task is complex; the decision maker is a domain expert; and when the decision environment has a high level of uncertainty, complexity, time pressure, insufficient data, and more than one reasonable solution. However, in employee selection, which is a decision environment that typically has the aforementioned characteristics that are conducive for intuition, scholars discount the usefulness of intuition in favor of more objective, analytical selection methods such as specific aptitude (e.g. sales ability) tests. A reason for the lack …

Mission Research

Journal of Adventist Mission Studies

No abstract provided.

Effects Of Ocean Warming, Sedimentation, And Parental Genotype On The Post-Settlement Survival And Growth Of Acropora Cervicornis Recruits, Jena Robbins

HCNSO Student Theses and Dissertations

Ocean warming and increased sedimentation from coastal activities are major threats to coral persistence. This study assessed the effects of increased temperature and sedimentation on the survival and growth of Acropora cervicornisrecruits. The potential for adults from different regions and genotypes to confer their offspring with higher or lower tolerance to heat and/or sediment was also determined. Gametes were collected and brought to the laboratory for cross fertilization of different genotypes within the regions collected. Larval rearing and settlement were then performed in the laboratory. Newly settled recruits were reared at 29 and 31°C (current summer temperature for August …

Path To Successful Diabetes Drug Trial Began With Simple Question, Jeff Hansen

Browse All News

No abstract provided.

The Role Of Caregiver Disruption In The Development Of Juvenile Sexual Offenders, Miranda Sitney

Dissertations and Theses

In the last decade, it has been recognized that juveniles commit as much as 20% of all sexual offenses in the United States (DOJ, 2004). Research that attempts to understand why young people commit sex crimes points to an array of family factors that may uniquely contribute to the development of sexual offending over and above general juvenile delinquency. This study specifically examines disrupted caregiving, or receiving insufficient or substitute care, as a potential moderator in the relationship between offense status and caregiver-child relationship quality. Four distinct moderators were tested: gender of caregiver, biological relationship between caregiver and child, number …

Methods And Algorithms For Inference Problems In Population Genetics, Jingwen Pei

Doctoral Dissertations

Inference of population history is a central problem of population genetics. The advent of large genetic data brings us not only opportunities on developing more accurate methods for inference problems, but also computational challenges. Thus, we aim at developing accurate method and fast algorithm for problems in population genetics.

Inference of admixture proportions is a classical statistical problem. We particularly focus on the problem of ancestry inference for ancestors. Standard methods implicitly assume that both parents of an individual have the same admixture fraction. However, this is rarely the case in real data. We develop a Hidden Markov Model (HMM) …

An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop In Plants., Stefan Sassmann, Cecilia Rodrigues, Stephen W. Milne, Anja Nenninger, Ellen Allwood, George R. Littlejohn, Nicholas J. Talbot, Christian Soeller, Brendan Davies, Patrick J. Hussey, Michael J. Deeks

School of Biological and Marine Sciences

Cell wall appositions (CWAs) are produced reactively by the plant immune system to arrest microbial invasion through the local inversion of plant cell growth. This process requires the controlled invagination of the plasma membrane (PM) in coordination with the export of barrier material to the volume between the plant PM and cell wall. Plant actin dynamics are essential to this response, but it remains unclear how exocytosis and the cytoskeleton are linked in space and time to form functional CWAs. Here, we show that actin-dependent trafficking to immune response sites of Arabidopsis thaliana delivers membrane-integrated FORMIN4, which in turn contributes …

An Analysis Of Innovate Training With Bottlenose Dolphins (Tursiops Truncatus), Raymond John Van Steyn

Theses and Dissertations

The National Aquarium in Baltimore, Maryland conducted a training program in 2014 to develop a gestural command for their dolphins called “innovate”. This training paradigm was developed to resemble the seminal research by Pryor, Haag and O’Reilly (1969), as well as more recent efforts of Braslau-Schneck (1993) and Kuczaj and Eskelinen (2014) of training dolphins to offer “creative” behaviors not developed through conventional methods of behavioral modification, such as shaping. The goal of the present study was to observe records taken during the National Aquarium’s training procedure as well as data collected ~3 years after said training in order to …

Alma Link Resolver Subject Report 2017-2018, Andrée J. Rathemacher

Technical Services Reports and Statistics

Statistics for 2017-2018 on the number of OpenURL requests by Library of Congress classification code made to the Ex Libris Alma link resolver for items held by the University of Rhode Island Libraries.

Information provided includes Classification Code, Classifications, Number of Requests, Number of Clicked Requests, and % Clicks from Requests.