Digital Commons Network^™

Examining The Legacy Of Transgenerational Trauma And Its Effects On Contemporary African American Adults In Parenting And Caregiver Roles To African American Adolescents, Amy E. Alexander

Electronic Theses and Dissertations

The system of institutional enslavement in North America has produced myriad effects on the contemporary African American community via the transmission of individual, familial, and collective trauma across generations (Carter, 2015; DeGruy, 2005; Weingarten, 2004). This research explored the roles of parent and caregiver within this cultural group in an effort to determine how past traumas have influenced the lived experiences and world view of African Americans in these roles.

While research on certain historically marginalized cultural and religious groups is plentiful, there is a clear lacuna of scholarly investigation into the African American experience, particularly as it relates to …

Alumna Establishes Endowment In Sister's Memory, Bill Dabney

University of Mississippi News

Gift will fund scholarships and other assistance for students in School of Applied Sciences

Re-Queering The Trans Binary: Gender Nonconforming Individuals’ Experiences In Counseling And Therapeutic Settings, Michael Stephens

Electronic Theses and Dissertations

This study sought to unearth the narratives of gender nonconforming (GNC) individuals’ experiences of mental health services. The term gender nonconforming refers to individuals who do not identify with a strictly male or female concept of gender identity. There is an insubstantial research that has been conducted into the provision of effective mental health services for gender nonconforming individuals. Most of the studies in the literature review used the term transgender to highlight gender minority experience of counseling.

This study used gender nonconforming to separate from this terminology confusion. Individuals who identify with the identity label of transgender can be …

Self-Assembling Bioaffinity Hydrogels For Localized Antibody Delivery, Wen Liu

Electronic Theses and Dissertations

The scope of illnesses that are being treated using monoclonal antibodies (mAbs) has been expanding from cancers to autoimmune diseases due to their high specificity to their molecular target. Because many of the new mAbs are aimed to modulate the immune system, an emerging concern is that patients with chronic illnesses are subjected to immune-related Adverse Events (irAEs). Loss of immunological tolerance to self-antigens or increased susceptibility to infections have been documented in some mAb treatments. Localized sustained release of mAb at disease sites might mitigate the risk of such toxicities. Antibody drugs are formulated typically for parenteral delivery because …

Ectopic Expression Of A Stilbenoid Prenyltransferase From Peanut In Hairy Roots Of Muscadine Grape, Mohammad Fazle Azim

Student Theses and Dissertations

Stilbenoids are phenolic compounds which not only serve as phytoalexins but also exhibit biological activities important to human health, including anticancer, anti-inflammatory and neuroprotective properties in vitro. However, some stilbenoids, such as the non prenylated stilbenoid resveratrol, have poor bioavailability limiting their application in vivo. Therefore, the goal of this project was to express a peanut stilbenoid prenyltransferase (i.e. AhR4DT-1) in muscadine grape to convert resveratrol to the prenylated stilbenoid arachidin-2, a potentially more bioavailable stilbenoid than resveratrol. Four hairy root lines of muscadine grape were developed via transformation with an engineered Agrobacterium rhizogenes harboring a peanut stilbenoid prenyltransferase. The …

Role Of Nucleotide Identity In Effective Crispr Target Escape Mutations, Tim Künne, Yifan Zhu, Fausia Da Silva, Nico Konstantinides, Rebecca E. Mckenzie, Ryan N. Jackson, Stan J.J. Brouns

Chemistry and Biochemistry Faculty Publications

Prokaryotes use primed CRISPR adaptation to update their memory bank of spacers against invading genetic elements that have escaped CRISPR interference through mutations in their protospacer target site. We previously observed a trend that nucleotide-dependent mismatches between crRNA and the protospacer strongly influence the efficiency of primed CRISPR adaptation. Here we show that guanine-substitutions in the target strand of the protospacer are highly detrimental to CRISPR interference and interference-dependent priming, while cytosine-substitutions are more readily tolerated. Furthermore, we show that this effect is based on strongly decreased binding affinity of the effector complex Cascade for guanine-mismatched targets, while cytosine-mismatched targets …

Psychological And Family Variables Associated With The Treatment Of Pediatric Idiopathic Pain, Rachael Hoffman

Electronic Theses and Dissertations

The present study examined variability in treatment outcomes of children with idiopathic chronic pain. Specifically, the biopsychosocial model was used as a framework to consider the way that symptoms of depression and anxiety as well as parent response to pain contributed to the change in a child’s confidence in participating in daily activities following treatment in an inpatient chronic pain treatment program.

Slippery Fish: Enforcing Regulation When Agents Learn And Adapt, Andres Gonzalez Lira, Ahmed Musfiq Mobarak

Cowles Foundation Discussion Papers

Attempts to curb undesired behavior through regulation gets complicated when agents can adapt to circumvent enforcement. We test a model of enforcement with learning and adaptation, by auditing vendors selling illegal fish in Chile in a randomized controlled trial, and tracking them daily using mystery shoppers. Conducting audits on a predictable schedule and (counter-intuitively) at high frequency is less effective, as agents learn to take advantage of loopholes. A consumer information campaign proves to be almost as cost-effective and curbing illegal sales, and obviates the need for complex monitoring and policing. The Chilean government subsequently chooses to scale up this …

Functional Genomics And Immunologic Tools: The Impact Of Viral And Host Genetic Variations On The Outcome Of Zika Virus Infection, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Aaron L. Olsen, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) causes no-to-mild symptoms or severe neurological disorders. To investigate the importance of viral and host genetic variations in determining ZIKV infection outcomes, we created three full-length infectious cDNA clones as bacterial artificial chromosomes for each of three spatiotemporally distinct and genetically divergent ZIKVs: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015). Using the three molecularly cloned ZIKVs, together with 13 ZIKV region-specific polyclonal antibodies covering nearly the entire viral protein-coding region, we made three conceptual advances: (i) We created a comprehensive genome-wide portrait of ZIKV gene products and their related species, with several previously …

Wellness And Karate, Cristina Kumpf

Electronic Theses and Dissertations

The promotion of wellness is an integral part of the counseling field and is considered to be a central focus to the counseling process (Barden, Conley, & Young, 2015; Myers, 1992). The counseling profession adopted the concept of wellness because it harmonizes with many of the founding principles of the ACA and is seen as a process of questing toward optimal health and well-being in body, mind, and spirit (Barden, Conley, & Young, 2015; Myers, 1992; Myers, Sweeney, & Witmer, 2000). Additionally, ACA Code of Ethics (2014) state that counselors “engage in self-care activities to maintain and promote their own …

Structure And Thermodynamics Of Polyglutamine Peptides And Amyloid Fibrils Via Metadynamics And Molecular Dynamics Simulations, Riley Workman

Electronic Theses and Dissertations

Aggregation of polyglutamine (polyQ)-rich polypeptides in neurons is a marker for nine neurodegenerative diseases. The molecular process responsible for the formation of polyQ fibrils is not well understood and represents a growing area of study. To enable development of treatments that could interfere with aggregation of polyQ peptides, it is crucial to understand the molecular mechanisms by which polyQ peptides aggregate into fibrils. Many experimental techniques have been employed to probe polyQ aggregation, however, observations from these studies have not lead to a unified understanding of the properties of these systems, instead yielding competing, fragmented theories of polyQ aggregation. This …

The Association Of Prescription Opioid Exposure And Patient Factors With Prolonged Postoperative Opioid Use In Opioid Naive Patients, Jennifer Lanzillotta

Electronic Theses and Dissertations

Background

Research suggests prolonged postoperative opioid use occurs in 4-13% of opioid naïve patients and is related to factors other than surgical pain. However, it is unclear which patient factors and prescribing practices are associated with prolonged use after surgery among opioid naïve patients.

Objectives

To identify factors associated with prolonged postoperative opioid use (refills 90-180 days after surgery) in opioid naïve patients in two domains: specific patient characteristics (demographics, smoking status, comorbidities, etc.) and exposure through postoperative opioid prescriptions (in oral morphine milligram equivalents [OME]).

Methods

An electronic medical record dataset analysis of inpatient and outpatient opioid naïve adult …

Gq Noncanonical Roles In Translational Regulation, Brett Demarco

Electronic Theses and Dissertations

This study investigates protein nucleic acid interactions, focusing on G-quadruplex (GQ) forming DNA/RNA in human disease. GQ structures are formed in DNA/RNA, when four guanine residues form planar tetrads stabilized by Hoogsteen base pairing, that stack forming a GQ structure stabilized by potassium ions. These GQ structures are targeted by the arginine glycine-glycine (RGG) RNA-binding domain. Fragile X mental retardation protein (FMRP), a translation regulator protein implicated in the fragile X syndrome, has an RGG domain and has been previously shown to interact with neuronal GQ forming messenger RNA (mRNA). We have investigated three neuronal FMRP mRNA targets that we …

E. Coli Elongation Factor Tu Bound To A Gtp Analogue Displays An Open Conformation Equivalent To The Gdp-Bound Form, Jesper S. Johansen, Darius Kavaliauskas, Shawn H. Pfeil, Mickael Blaise, Barry C. Cooperman, Yale E. Goldman, Søren S. Thirup, Charlotte R. Knudsen

Physics & Engineering Faculty Publications

According to the traditional view, GTPases act as molecular switches, which cycle between distinct ‘on’ and ‘off’ conformations bound to GTP and GDP, respectively. Translation elongation factor EF-Tu is a GTPase essential for prokaryotic protein synthesis. In its GTP-bound form, EF-Tu delivers aminoacylated tRNAs to the ribosome as a ternary complex. GTP hydrolysis is thought to cause the release of EF-Tu from aminoacyl-tRNA and the ribosome due to a dramatic conformational change following Pi release. Here, the crystal structure of Escherichia coli EF-Tu in complex with a non-hydrolysable GTP analogue (GDPNP) has been determined. Remarkably, the overall conformation of EF-Tu·GDPNP …

Post-Treatment Effects Of Infrared Radiation On Rough Rice Germination And Lesser Grain Borers (Rhyzopertha Dominica (F.)), Rachel Marie Hampton

Student Theses and Dissertations

Infrared radiation (IR) has been shown to effectively dry grain while eliminating stored-product insects. Little has been done to examine IR’s effect on rice germination or insect development. Two studies were conducted using XL745 (hybrid) and CL152 (pureline) rice cultivars dried with three IR intensities (2.15, 2.83, and 10.84 kW/m2). Control samples were dried in an environmental chamber (0 kW/m2). Rice was germinated for 14 days. An IR intensity of 10.84 kW/m2 reduced germination, shoot and primary root lengths compared to lower intensities. Lesser grain borers (LGB), Rhyzopertha dominica (F.), were placed on treated rough and brown rice for 50 …

Preparation And Characterization Of Arabinoxylan From Rice Bran Fiber, Divya Kandanool

Student Theses and Dissertations

The purpose of this investigation was to isolate arabinoxylan (AX) from rice bran fiber and characterize its structural properties with enzyme and chromatographic techniques. The hypothesis was that glycoside hydrolase family 11 endo-xylanase (GH11-XYN) action on rice bran AX would generate oligosaccharide profiles that are distinguishable from other cereal species. The Specific Objectives addressed were to: 1) Extract AX fractions from rice bran fiber and determine yield and compositions. 2) Treat rice bran AX fractions with defined XYNs, and then characterize the enzyme-product profiles. Results showed that GH11-XYN generated large fragments from rice AX (average degree of polymerization 25 to …

Management Implications Of Sex-Specific Habitat Use By Nycticeius Humeralis In North-Central Arkansas, Megan Lynn Buckley

Student Theses and Dissertations

The goal of this study was to evaluate evening bat (Nycticeius humeralis) habitat use and provide a proxy strategy to evaluate habitat use by other species. I used the software Maximum Entropy to evaluate habitat suitability models for evening bats using three different modeling strategies; percent variable contribution, the jackknife test, and variable response curves. The predictors included in the models were 10 land use land cover classes, four forest management strategies, slope, and the normalized difference vegetation index (NDVI). The results of this research showed that distance to burned stands and proximity to edge habitat are the most important …

Synthesis Of Modified Biginelli Compounds, Michelle Sarah Collins

Student Theses and Dissertations

The Biginelli reaction has been a distinguished reaction for many years. Their products, the Dihydropyrimidones have been shown to contain a vast array of bioactivity especially when they contain other known bioactive functional groups. Here in we discuss different pathways that these DHPM products can be taken down to combine them with other such bioactive functional groups. We explored the addition of alkene and aldehyde groups to the Biginelli compounds in hopes of synthesizing potential drug candidates. We believe the synthesis of the aldehyde group on the DHPM compounds is a gateway to obtaining the hybrid drug activity we so …

The Zombie Apocalypse Has Already Happened: Queering Subjectivity In Zombie Film, Jessica Dunn

Electronic Theses and Dissertations

This dissertation traces the encounter between psychology as a human science and the viral zombie film genre using the collaborative works of Deleuze and Guattari in conjunction with the queer anti-humanist theories of Colebrook and Halberstam. I interwove thematic (i.e. plot, dialogue, character development) and cinematographic (i.e. shot composition, editing, lighting, musical score) analyses of fourteen viral zombie films with theoretical arguments regarding the deterritorialization of the human subject and its relevance to psychology as a human science. The films were selected from the plethora of viral zombie films released after the turn of the 21st century. The selection was …

Back In The Closet: A Queer-Modified Interpretive-Phenomenological Analysis Of Gay Men Creating An Identity Through Clothing, Jose Arroyo

Electronic Theses and Dissertations

How one dresses comprises their personal style, which subsequently informs one’s orientation to the world. Clothing is a global practice that demarcates our experience of self, others, and the world; and—as we are swathed in clothing since birth—those experiences are primarily split among gendered lines. Using Merleau-Ponty (2014/1945), I reconceptualize how clothing can be understood as inseparable from our body, and I use Deleuze and Guattari (1987/1980) to illustrate how clothing augments our embodied experience to produce a process of identification with our style. I also draw from queer theorists (Ahmed, 2006; Halberstam, 2011) to illustrate how we can disrupt …

End-Of-Life Care Practices In Two Cultures: A Comparative Ethical Analysis And Recommendations, Nora Alharbi

Electronic Theses and Dissertations

ABSTRACT

This dissertation examined the similarities and differences between the end-of-life care strategies employed in the United States and Saudi Arabia. End-of-life care has emerged in recent decades as a medical practice specialty. It has taken on enhanced significance in the United States in tandem with the aging of the Baby Boomer generation, but it is also a significant focus of practice and research in other countries as well. In the context of Saudi Arabia, however, end-of-life care is still very much an emerging phenomenon that is being shaped and informed by Saudi culture, tradition and the Islamic faith.

This …

Groundwater Flow And Exchange Across The Land Surface Explain Carbon Export Patterns In Continuous Permafrost Watersheds, Bethany T. Neilson, M. Bayani Cardenas, Michael T. O'Connor, Mitchell T. Rasmussen, Tyler V. King, George W. Kling

Civil and Environmental Engineering Faculty Publications

Groundwater flow regimes in the seasonally thawed soils in areas of continuous permafrost are relatively unknown despite their potential role in delivering water, carbon, and nutrients to streams. Using numerical groundwater flow models informed by observations from a headwater catchment in arctic Alaska, United States, we identify several mechanisms that result in substantial surface‐subsurface water exchanges across the land surface during downslope transport and create a primary control on dissolved organic carbon loading to streams and rivers. The models indicate that surface water flowing downslope has a substantial groundwater component due to rapid surface‐subsurface exchanges across a range of hydrologic …

The Effectiveness Of Best Management Practices On Water Quality In The Attoyac Bayou Watershed Of East Texas, Cheryl Scott

Electronic Theses and Dissertations

In the Attoyac Bayou Watershed Protection Plan, best management practices (BMPs) were developed for livestock, wildlife, and on-site sewage facilities (OSSFs), using bacteria source tracking (BST), spatially explicit load enrichment calculation tool (SELECT) model, as well as a cost benefit analysis. An evaluation of these BMPs was conducted on the Attoyac Bayou (AB), Big Iron Ore Creek (BIO), Naconiche Creek (NC), Terrapin Creek (TC), and Waffelow Creek (WC) to see if they were meeting their designated use criteria for elevated Escherichia coli (E. coli). Water quality parameters were monitored for five sample locations within the Attoyac Bayou Watershed during March …

Characterization Of Sites For Native Herbaceous Understory Restoration In West Gulf Coast Longleaf Pine (Pinus Palustris) Savannas, Brooke Mccalip

Electronic Theses and Dissertations

Longleaf pine (Pinus palustris) savannas were once dominant in East Texas and parts of western and central Louisiana. Native understory species have since been removed or reduced by exotic plants that were introduced and from the reduction in the frequency of both wild and prescribed fires. A diverse layer of understory species can still be seen today, but not often in the historical savanna setting that is desirable in longleaf pine ecosystems. This project aimed to identify site characteristics associated with longleaf ecosystems that support a dense, herbaceous understory with little to no midstory cover.

A total of …

Characterization Of Sites For Native Herbaceous Understory Restoration In West Gulf Coast Longleaf Pine (Pinus Palustris) Savannas, Brooke Mccalip

Electronic Theses and Dissertations

Longleaf pine (Pinus palustris) savannas were once dominant in East Texas and parts of western and central Louisiana. Native understory species have since been removed or reduced by exotic plants that were introduced and from the reduction in the frequency of both wild and prescribed fires. A diverse layer of understory species can still be seen today, but not often in the historical savanna setting that is desirable in longleaf pine ecosystems. This project aimed to identify site characteristics associated with longleaf ecosystems that support a dense, herbaceous understory with little to no midstory cover.

A total of …

Kadar Pati Akar Dan Sitokinin Endogen Pada Tanaman Teh Menghasilkan Sebagai Dasar Penentuan Pemangkasan Dan Aplikasi Zat Pengatur Tumbuh, Intan Ratna Dewi Anjarsari, Jajang Sauman Hamdani, Cucu Suherman, Tati Nurmala, Heri Syahrian Khomaeni, Vitria Puspitasari Rahadi

Jurnal Kultivasi

Pemangkasan pada tanaman teh dilakukan salah satunya untuk menginisiasi tumbuhnya banyak tunas sebagai bakal pembentukan pucuk peko. Pemangkasan mengubah luas daun, kapasitas fotosintesis perdu, mempengaruhi keseimbangan metabolisme antara organ di atas dan di bawah tanah dengan mengurangi jumlah tumbuh tunas yang berfungsi sebagai sumber dan pengguna untuk nutrisi dan hormon. Sampai saat ini pertumbuhan tunas sebagai bakal daun setelah pemangkasan terjadi secara alami tanpa penambahan zat pengatur tumbuh (ZPT). Pada dasarnya rekayasa fisiologis dengan menggunakan ZPT sitokinin dapat menjadi pilihan untuk lebih memacu pertumbuhan cabang lateral dan tunas serta memecahkan dormansi pucuk. Tujuan penelitian pendahuluan ini adalah untuk mengetahui kadar …

Pemanfaatan Beberapa Jenis Urin Ternak Sebagai Pupuk Organik Cair Dengan Konsentrasi Yang Berbeda Pada Tanaman Kelapa Sawit (Elaeis Guineensis Jacq.) Di Pembibitan Utama, Bisri Alvi, Mira Ariyanti, Yudithia Maxiselly

Jurnal Kultivasi

Pembibitan merupakan tahapan awal dalam budidaya tanaman kelapa sawit, kualitas bibit akan mempengaruhi hasil yang akan diperoleh nantinya. Faktor yang mempengaruhi pertumbuhan bibit diantaranya adalah ketersediaan unsur hara yang dapat diperoleh dari pemberian pupuk anorganik dan organik. Pemberian pupuk anorganik tanpa diimbangi pupuk organik dapat merusak sifat tanah, sehingga diperlukan pupuk organik yaitu dengan memanfaatkan urin ternak sebagai pupuk organik cair, dengan menambahkan pupuk organik cair pada tanah, maka dapat membantu proses pertumbuhan tanaman karena pupuk organik cair urin ternak mengandung hormon pertumbuhan bagi tanaman serta mudah diserap tanaman. Penelitian dilakukan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Kabupaten …

Economics Of Managing Loblolly Pine Plantations For Increased Wildlife Biodiversity, Melissa Griffith

Electronic Theses and Dissertations

The goal of this thesis was to identify the differences between the costs incurred and revenues gained from managing loblolly pine (Pinus taeda) plantations in East Texas for strictly timber production or a combination of timber production and wildlife biodiversity. Management regimes evaluated had varying planting densities, thinning frequency and intensities, prescribed burn intervals, and site indices. Economic analyses used to evaluate management regimes over a range of nonindustrial private landowner (NIPF) alternative rates of return (ARRs) and site indexes were net present worth, land expectation value, and equal annual annuity. These economic analyses demonstrated that altering management …

Breeding Bird Response To Post Oak Savanna Restoration Seven Years Post Management In Eastern Texas, Courtney Mcinnerney

Electronic Theses and Dissertations

Oak savannas were once an abundant vegetation type in the Midwestern United States that have now declined to <1% of their original distribution. Historically, natural disturbances such as periodic fire and grazing maintained oak savannas, but these have been reduced or eliminated, resulting in woody encroachment and subsequent habitat loss and degradation. In 2009-10, a baseline, pre-restoration study was completed to determine vegetation characteristics, breeding bird abundances, nest success, and nest site selection at the Gus Engeling Wildlife Management Area (GEWMA) in eastern Texas. The results showed a lack of savanna vegetation structure on degraded sites and few savanna or grassland obligate bird species. The goal of this study was to determine how breeding birds of oak savanna vegetation types in eastern Texas respond to restoration effects 7 years after initial management. Post-restoration surveys completed in 2016-17 showed a change in avian assemblages from a more woodland dominated community to grassland/savanna community. The presence and breeding of savanna obligate species dickcissel (Spiza americana) and lark sparrow (Chondestes grammacus) indicates that the restoration was successful. The presence of savanna species can be linked to the herbaceous vegetation that was restored to more closely resemble historic oak savanna structure and can quantify the success of restoration efforts.

Clinical Presentation Non-Cme: How Voltage Controls Oxygen Levels In Health And Disease, Jerry Tennant

International Symposium for Hyperbaric Medicine

As a world leading expert on healing and voltage, and developer of the Tennant Biomodulator frequencies, Dr. Jerry Tennant, will be discussing “How Voltage Controls Oxygen Levels in Health and Disease”.