Digital Commons Network^™

The Role Of Human Papillomaviruses And Polyomaviruses In Braf-Inhibitor Induced Cutaneous Squamous Cell Carcinoma And Benign Squamoproliferative Lesions, Karin J. Purdie, Charlotte M. Proby, Hasan Rizvi, Heather Griffin, John Doorbar, Mary Sommerlad, Mariet C. Feltkamp, Els Van Der Meijden, Gareth J. Inman, Andrew P. South, Irene M. Leigh, Catherine A. Harwood

Department of Dermatology and Cutaneous Biology Faculty Papers

Background: Human papillomavirus (HPV) has long been proposed as a cofactor in the pathogenesis of cutaneous squamous cell carcinoma (cSCC). More recently, the striking clinico-pathological features of cSCCs that complicate treatment of metastatic melanoma with inhibitors targeting BRAF mutations (BRAFi) has prompted speculation concerning a pathogenic role for oncogenic viruses. Here, we investigate HPV and human polyomaviruses (HPyV) and correlate with clinical, histologic, and genetic features in BRAFi-associated cSCC. Materials and Methods: Patients receiving BRAFi treatment were recruited at Barts Health NHS Trust. HPV DNA was detected in microdissected frozen samples using reverse line probe technology and degenerate and nested …

Immune Cells That Create And Sustain Chronic Inflammatory Bowel Disease Identified, Brit Blalock

Browse All News

No abstract provided.

Fars2 Mutations Presenting With Pure Spastic Paraplegia And Lesions Of The Dentate Nuclei, Supreet K. Sahai, Rebecca E. Steiner, Margaret G. Au, John M. Graham, Norikio Salamon, Michael Ibba, Tyler M. Pierson

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Mutations in FARS2, the gene encoding the mitochondrial phenylalanine‐tRNA synthetase (mtPheRS), have been linked to a range of phenotypes including epileptic encephalopathy, developmental delay, and motor dysfunction. We report a 9‐year‐old boy with novel compound heterozygous variants of FARS2, presenting with a pure spastic paraplegia syndrome associated with bilateral signal abnormalities in the dentate nuclei. Exome sequencing identified a paternal nonsense variant (Q216X) lacking the catalytic core and anticodon‐binding regions, and a maternal missense variant (P136H) possessing partial enzymatic activity. This case confirms and expands the phenotype related to FARS mutations with regards to clinical presentation and neuroimaging findings.

Anesthesia For The Patient With Mast Cell Activation Disease, Chelsey G. Horner

Nursing Capstones

No abstract provided.

Repeatability And Reliability Of Exploratory Behavior In Proactive And Reactive Zebrafsh, Danio Rerio, Matthew R. Baker, Alexander C. Goodman, Jonathan B. Santo, Ryan Y. Wong

Biology Faculty Publications

Behavioral responses to novel situations often vary and can belong to a suite of correlated behaviors. Characteristic behaviors of different personality types (e.g. stress coping styles) are generally consistent across contexts and time. Here, we compare the repeatability and reliability of exploratory behaviors between zebrafsh strains selectively bred to display contrasting behavioral responses to stressors that represent the proactive-reactive axis. Specifically, we measure exploratory behavior of individual fish in an open field test over five weeks. We quantified the stationary time, average swimming speed and time spent by a fish in the center area. We found a number of strain …

Forensic Chemist Named 2018 Joseph Sam Distinguished Alumnus, Whitney Tarpy

University of Mississippi News

Randall Clark earned his doctorate in medicinal chemistry from the School of Pharmacy

Racing On Two Different Tracks: Using Substantive Due Process To Challenge Tracking In Schools, Katarina Wong

Duke Journal of Constitutional Law & Public Policy Sidebar

Tracking is a widespread educational practice where secondary schools divide students into different classes or “tracks” based on their previous achievements and perceived abilities. Tracking produces different levels of classes, from low ability to high ability, based on the theory that students learn better when grouped with others at their own level. However, tracking often segregates students of color and low socioeconomic status into low-tracked classes and these students do not receive the same educational opportunities as white and/or wealthier students. Students and parents have historically challenged tracking structures in their schools using an Equal Protection Clause framework. However, this …

Molecular Determinants Of Substrate Specificity In Human Insulin-Degrading Enzyme, Lazaros Stefanidis, Nicholas D. Fusco, Samantha E. Cooper, Jilian E. Smith-Carpenter, Benjamin J. Alper

Chemistry & Physics Faculty Publications

Insulin-degrading enzyme (IDE) is a 110 kDa chambered zinc metalloendopeptidase that degrades insulin, amyloid beta, and other intermediate-sized aggregation prone peptides that adopt β-structures. Structural studies of IDE in complex with multiple physiological substrates have suggested a role for hydrophobic and aromatic residues of the IDE active site in substrate binding and catalysis. Here, we examine functional requirements for conserved hydrophobic and aromatic IDE active site residues that are positioned within 4.5 Angstroms of IDE bound insulin B chain and amyloid beta peptides in the reported crystal structures for the respective enzyme-substrate complexes. Charge, size, hydrophobicity, aromaticity, and other functional …

Japanese Beetles’ Feeding On Milkweed Flowers May Compromise Efforts To Restore Monarch Butterfly Habitat, Adam M. Baker, Daniel A. Potter

Entomology Faculty Publications

The eastern North American migratory population of monarch butterflies (Danaus plexippus) is in serious decline. Habitat restoration, including adding millions of host plants to compensate for loss of milkweed in US cropland, is a key part of the international conservation strategy to return this iconic butterfly to sustainable status. We report here that Popillia japonica, a polyphagous, invasive beetle, aggregates and feeds on flowers of Asclepias syriaca, the monarch’s most important larval food plant, reducing fruiting and seed set by >90% and extensively damaging milkweed umbels in the field. The beetle’s ongoing incursion into the monarch’s …

Double-Stranded Rna Binding Protein, Staufen, Is Required For The Initiation Of Rnai In Coleopteran Insects, June-Sun Yoon, Kanakachari Mogilicherla, Dhandapani Gurusamy, Xien Chen, Shankar C. R. R. Chereddy, Subba R. Palli

Entomology Faculty Publications

RNA interference (RNAi) is being used to develop methods to control pests and disease vectors. RNAi is robust and systemic in coleopteran insects but is quite variable in other insects. The determinants of efficient RNAi in coleopterans, as well as its potential mechanisms of resistance, are not known. RNAi screen identified a double-stranded RNA binding protein (StaufenC) as a major player in RNAi. StaufenC homologs have been identified in only coleopteran insects. Experiments in two coleopteran insects, Leptinotarsa decemlineata and Tribolium castaneum, showed the requirement of StaufenC for RNAi, especially for processing of double-stranded RNA (dsRNA) to small interfering …

Revision Of Zosteragathis Sharkey Of Thailand (Hymonoptera, Braconidae, Agathidinae, Agathidini), Michael J. Sharkey, Eric G. Chapman

Entomology Faculty Publications

Based on cladistic analyses recently conducted by Sharkey and Chapman, the genus Zosteragathis Sharkey is revised. Twenty-two species are reported from Thailand, three previously described species, Z. samensis, Z. contrasta and Z. nuichuaensis, and 19 new species, i.e., Z. chaiyaphumensis, Z. eukos, Z. hinensis, Z. hongensis, Z. inthanonensis, Z. krachanensis, Z. lampangensis, Z. lampooensis, Z. luangensis, Z. ngamensis, Z. perknos, Z. petchaburiensis, Z. phahompokensis, Z. phuphanensis, Z. sakaeratensis, Z. sakonensis, Z. samensis, Z. surinensis, Z. taemensis, Z. tonensis. …

Orthoquery: A Tripal Database Module To Assess And Visualize Gene Family Evolution, Sumaira Zaman

Master's Theses

No abstract provided.

Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone

Biology Faculty Articles

Objectives

The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi‐tropical sites in Tanzania, Mexico, and Puerto Rico (N = 12).

Materials and Methods

All samples were extracted twice, built into double‐stranded sequencing libraries, and shotgun sequenced on the Illumina HiSeq 2500. The first extraction protocol, Method D, was previously designed for …

The Role Of Toll-Like Receptor 2 And 4 Innate Immunity Pathways In Intracortical Microelectrode-Induced Neuroinflammation, John K. Hermann, Shushen Lin, Arielle Soffer, Chun Wong, Vishnupriya Srivastava, Jeremy Chang, Smrithi Sunil, Shruti Sudhakar, William H. Tomaszewski, Grace Protasiewicz, Stephen M. Selkirk, Jeffrey R. Capadona

Faculty Scholarship

We have recently demonstrated that partial inhibition of the cluster of differentiation 14 (CD14) innate immunity co-receptor pathway improves the long-term performance of intracortical microelectrodes better than complete inhibition. We hypothesized that partial activation of the CD14 pathway was critical to a neuroprotective response to the injury associated with initial and sustained device implantation. Therefore, here we investigated the role of two innate immunity receptors that closely interact with CD14 in inflammatory activation. We implanted silicon planar non-recording neural probes into knockout mice lacking Toll-like receptor 2 (Tlr2-/-), knockout mice lacking Toll-like receptor 4 (Tlr4-/-), and wildtype (WT) control mice, …

Combined Use Of Oscillating Means And Ellipsometry To Determine Uncorrelated Effective Thickness And Optical Constants Of Material Deposited At Or Etched From A Working Electrode That Preferrably Comprises Non - Normal Oriented Nanofibers, Mathias M. Schubert, Tino Hofmann, John A. Woollam, Rebecca Y. Lai

Department of Electrical and Computer Engineering: Faculty Publications

Disclosed are systems and methods that enable determination of uncorrelated thickness of a working electrode and surface region optical constants in settings involving electrochemical processing at a working electrodes in a Piezo electric Balance system , by simultaneous application of an Ellipsometer system , the working electrode optionally having a multiplicity of nanofibers that are oriented non normally to a surface of said working electrode . Further disclosed is , simultaneous with said determinations , the monitoring of electrochemical processes at a piezoelectric balance working electrode driven by electrical energy applied between said working electrode and counter electrode .

Comparison Of Motor Skill Learning, Grip Strength And Memory Recall On Land And In Chest-Deep Water, Eadric Bressel, Michiel N. Vakula, Youngwook Kim, David A.E. Bolton, Chris J. Dakin

Kinesiology and Health Science Faculty Publications

Immersion in chest-deep water may augment explicit memory in healthy adults however, there is limited information on how this environment might affect implicit memory or motor learning. The purpose of this study was to compare the speed and accuracy for learning a motor skill on land and in chest-deep water. Verbal word recall and grip strength were included to gain a more complete understanding of the intervention. Sixty-two younger adults (age = 23.3 ± 3.59 yrs.) were randomly assigned to either a water group immersed to the xiphoid or a land group. Participants in both groups completed the same eight …

Hgf-Induced Formation Of The Met–Axl–Elmo2–Dock180 Complex Promotes Rac1 Activation, Receptor Clustering, And Cancer Cell Migration And Invasion, Wenjing Li, Xiahui Xiong, Amro Abdalla, Salvador Alejo, Linyu Zhu, Fei Lu, Hong Sun

Chemistry and Biochemistry Faculty Research

The MET proto-oncogene–encoded receptor tyrosine kinase (MET) and AXL receptor tyrosine kinase (AXL) are independently operating receptor tyrosine kinases (RTKs) that are functionally associated with aggressive and invasive cancer cell growth. However, how MET and AXL regulate the migratory properties of cancer cells remains largely unclear. We report here that the addition of hepatocyte growth factor (HGF), the natural ligand of MET, to serum-starved human glioblastoma cells induces the rapid activation of both MET and AXL and formation of highly polarized MET–AXL clusters on the plasma membrane. HGF also promoted the formation of the MET and AXL protein complexes and …

Schizophrenia In Digeorge Syndrome: A Unique Case Report, Sukaina Rizvi, Ali M. Khan, Hina Saeed, Akeem M. Aribara, Alexis Carrington, Alexa Griffiths, Abdul Mohit

School of Medicine Publications

Herein we present the unique case of a 21-year-old African American woman who presented with psychotic features and the incidental finding of basal ganglia calcifications on computed tomography (CT) scan of the head. She was initially presumed to have Fahr’s syndrome in the context of idiopathic bilateral basal ganglia calcifications and psychotic features. Genetic testing performed revealed the deletion of 22q11.2, thus establishing the diagnosis of DiGeorge syndrome. This case highlights the importance of noticing subtle physical exam findings along with laboratory findings as this led to the diagnosis of DiGeorge syndrome for this patient. This case is unique in …

Third Generation Gamma Camera Spect System, Narayan Bhusal

LSU Master's Theses

Single Photon Emission Computed Tomography (SPECT) is a non-invasive imaging modality, frequently used in myocardial perfusion imaging. The biggest challenges facing the majority of clinical SPECT systems are low sensitivity, poor resolution, and the relatively high radiation dose to the patient. New generation systems (GE Discovery, DSPECT) dedicated to cardiac imaging improve sensitivity by a factor of 5-8. The purpose of this work is to investigate a new gamma camera design with 21 hemi-ellipsoid detectors each with a pinhole collimator for Cardiac SPECT for further improvement in sensitivity, resolution, imaging time, and radiation dose.

To evaluate the resolution of our …

Multi-Beam Scan Analysis With A Clinical Linac For High Resolution Cherenkov-Excited Molecular Luminescence Imaging In Tissue., Mengyu Jeremy Jia, Peter Bruza, Lesley A. Jarvis, David J. Gladstone, Brian W. Pogue

Dartmouth Scholarship

Cherenkov-excited luminescence scanned imaging (CELSI) is achieved with external beam radiotherapy to map out molecular luminescence intensity or lifetime in tissue. Just as in fluorescence microscopy, the choice of excitation geometry can affect the imaging time, spatial resolution and contrast recovered. In this study, the use of spatially patterned illumination was systematically studied comparing scan shapes, starting with line scan and block patterns and increasing from single beams to multiple parallel beams and then to clinically used treatment plans for radiation therapy. The image recovery was improved by a spatial-temporal modulation-demodulation method, which used the ability to capture simultaneous images …

Taxon Cycle Predictions Supported By Model-Based Inference In Indo-Pacific Trap-Jaw Ants (Hymenoptera: Formicidae: Odontomachus), Pável Matos-Maraví, Nicholas J. Matzke, Fredrick J. Larabee, Ronald M. Clouse, Ward C. Wheeler, Daniela Magdalena Sorger, Andrew V. Suarez, Milan Janda

Faculty Research, Scholarly, and Creative Activity

Nonequilibrium dynamics and non-neutral processes, such as trait-dependent dispersal, are often missing from quantitative island biogeography models despite their potential explanatory value. One of the most influential nonequilibrium models is the taxon cycle, but it has been difficult to test its validity as a general biogeographical framework. Here, we test predictions of the taxon cycle model using six expected phylogenetic patterns and a time-calibrated phylogeny of Indo-Pacific Odontomachus (Hymenoptera: Formicidae: Ponerinae), one of the ant genera that E.O. Wilson used when first proposing the hypothesis. We used model-based inference and a newly developed trait-dependent dispersal model to jointly estimate ancestral …

Rare Diatom Stauroneis Schinzii (Brun) Cleve Var. Schinzii Microfossil Collected And Georeferenced In Alabama, Jay Y.S. Hodgson, Amelia K. Ward, Kristopher D. Maedke-Russell

Georgia Journal of Science

The diatom Stauroneis schinzii (Brun) Cleve var. schinzii has an underreported record of its autecology, distribution, and natural history. While the species is found in Africa and South America, it is rare in North America and very few specimens have been collected and cataloged. As part of a landscape evolution study, we cored the sediments of an oxbow lake in the coastal plain of Alabama, USA, for diatom analysis. Among 40,608 individual diatoms sampled, we counted a single valve of S. s. var. schinzii, georeferenced its location, and recorded a micrograph. Based on the carbon dating of the sediment …

The Bello Chavez Family: A Reflection Of Health In The Mexican American Community Through A Family Nursing Assessment, Vanessa Bello Chavez

Best Integrated Writing

Vanessa Bello Chavez provides an in-depth, personal look into the health problems that affect Hispanic families across the United States. The writing takes a personal view and makes it relatable to all.

Best Integrated Writing 2018 - Complete Edition

Best Integrated Writing

Best Integrated Writing includes excellent student writing from Integrated Writing courses taught at Wright State University. The journal is published annually by the Wright State University Department of English Language and Literatures.

Chemokine Network And Overall Survival In Tp53 Wild-Type And Mutant Ovarian Cancer, Rosa Mistica C. Ignacio, Eun-Sook Lee, Andrew J. Wilson, Alicia Beeghly-Fadiel, Margaret M. Whalen, Deok-Soo Son

Chemistry Faculty Research

Ovarian cancer (OC) has the highest mortality rate among gynecological malignancies. Because chemokine network is involved in OC progression, we evaluated associations between chemokine expression and survival in tumor suppressor protein p53 (TP53) wild-type (TP53WT) and mutant (TP53m) OC datasets. TP53 was highly mutated in OC compared to other cancer types. Among OC subtypes, CXCL14 was predominantly expressed in clear cell OC, and CCL15 and CCL20 in mucinous OC. TP53WT endometrioid OC highly expressed CXCL14 compared to TP53m, showing better progression-free survival but no difference in overall survival (OS). TP53m serous …

Unh Research Finds Early-Season Heating Shows Promise Boosting Ginger Harvest, Lori Tyler Gula

Media Relations

No abstract provided.

Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic flavivirus, is an enveloped positive-strand RNA virus that can cause a spectrum of clinical manifestations, ranging from mild febrile illness to severe neuroinvasive disease. Today, several killed and live vaccines are available in different parts of the globe for use in humans to prevent JEV-induced diseases, yet no antivirals are available to treat JEV-associated diseases. Despite the progress made in vaccine research and development, JEV is still a major public health problem in southern, eastern, and southeastern Asia, as well as northern Oceania, with the potential to become an emerging global pathogen. In …

Network Changes Associated With Transdiagnostic Depressive Symptom Improvement Following Cognitive Behavioral Therapy In Mdd And Ptsd, Steven Bruce, Zhen Yang, Shi Gu, Nicolas Honnorat, Kristin Linn, Russell Shinohara, Irem Aselcioglu, Desmond Oathes, Christos Davatzikos, Theodore Satterthwaite, Danielle Bassett, Yvette Sheline

Psychology Faculty Works

Despite widespread use of cognitive behavioral therapy (CBT) in clinical practice, its mechanisms with respect to brain networks remain sparsely described. In this study, we applied tools from graph theory and network science to better understand the transdiagnostic neural mechanisms of this treatment for depression. A sample of 64 subjects was included in a study of network dynamics: 33 patients (15 MDD, 18 PTSD) received longitudinal fMRI resting state scans before and after 12 weeks of CBT. Depression severity was rated on the Montgomery-Asberg Depression Rating Scale (MADRS). Thirty-one healthy controls were included to determine baseline network roles. Univariate and …

Adviser Nomination Spurs Questions, Aldemaro Romero Jr.

Publications and Research

After a 19-month delay, the Trump Administration has nominated someone to be the director of the Office of Science and Technology Policy, or as is more colloquially known, the science adviser to the president.

Congress established the White House Office of Science and Technology Policy (OSTP) in 1976. President Gerald Ford, a Republican, signed the act creating the agency into law. That took place after President Nixon disbanded the then-called “President Advisory Committee” in 1973.

The mandate for the agency is to provide the president and others within the Executive Office of the President with advice on the scientific, engineering, …

Matlack Graduate Students Present At Ecological Society Of America, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.