Digital Commons Network^™

Landscape And Climate Drivers Of Harmful Algal Blooms In Iowa, Aaron Padilla

Dissertations and Theses @ UNI

Insuring the safety of our water for consumption and recreation is a crucial but extremely difficult and resource intensive activity of environmental protection agencies. One of the ways our water is at risk is through the increased frequency, and intensity of harmful algal blooms (HABs). When CyanoHABs occur, they can produce a toxic byproduct, most notably microcystin in freshwater, which can be harmful to humans and animals. Previous research has indicated these blooms are driven by increased eutrophication of water bodies, the excess loading of nutrients. In Iowa, the main driver of CyanoHABs in most watersheds is agricultural runoff, which …

Application Of Microrna Markers For Early Detection Of Latent Tuberculosis Transitioning To Active Tuberculosis, Robert A. Ollar

NYMC Faculty Publications

No abstract provided.

Phase 1 Study Of High-Specific-Activity I-131 Mibg For Metastatic And/Or Recurrent Pheochromocytoma Or Paraganglioma, Richard B. Noto, Daniel A. Pryma, Jessica Jensen, Tess Lin, Nancy Stambler, Thomas Strack, Vivien Wong, Stanley J. Goldsmith

NYMC Faculty Publications

Context: No therapies are approved for the treatment of metastatic and/or recurrent pheochromocytoma or paraganglioma (PPGL) in the United States. Objective: To determine the maximum tolerated dose (MTD) of high-specific-activity I-131 meta-iodobenzylguanidine (MIBG) for the treatment of metastatic and/or recurrent PPGL. Design: Phase 1, dose-escalating study to determine the MTD via a standard 3 + 3 design, escalating by 37 MBq/kg starting at 222 MBq/kg. Setting: Three centers. Patients: Twenty-one patients were eligible, received study drug, and were evaluable for MTD, response, and toxicity. Intervention: Open-label use of high-specific-activity I-131 MIBG therapy. Main Outcome Measures: Dose-limiting toxicities, adverse events, radiation …

Ruxolitinib Significantly Enhances In Vitro Apoptosis In Hodgkin Lymphoma And Primary Mediastinal B-Cell Lymphoma And Survival In A Lymphoma Xenograft Murine Model, Sanghoon Lee, Tishi Shah, Changhong Yin, Jessica Hochberg, Janet Ayello, Carmella Van De Ven, Mitchell S. Cairo

NYMC Faculty Publications

Hodgkin lymphoma (HL) and primary mediastinal B-cell lymphoma (PMBL) share similar molecular features by gene expression profiling. Frequent gains of chromosome 9p exhibit higher Janus Kinase 2 (JAK2) transcript levels with increased JAK2 activity, suggesting aberrant activity of JAK2 and STAT pathways. This signaling pathway alteration may in part play an important role in the pathogenesis and/or chemoradiotherapy resistance in HL and PMBL. Ruxolitinib is a potent and selective JAK1/JAK2 inhibitor, with activity against myeloproliferative neoplasms (MPNs) including those harboring the JAK2V617F mutation. We investigated the in vitro and in vivo efficacy of ruxolitinib and changes in downstream signaling pathways …

Estrogen-Dependent Epigenetic Regulation Of Soluble Epoxide Hydrolase Via Dna Methylation, Yang-Ming Yang, Dong Sun, Sharath Kandhi, Ghezal Froogh, Jian Zhuge, Wei-Hua Huang, Bruce D. Hammock, An Huang

NYMC Faculty Publications

To elucidate molecular mechanisms responsible for the sexually dimorphic phenotype of soluble epoxide hydrolase (sEH) expression, we tested the hypothesis that female-specific down-regulation of sEH expression is driven by estrogen-dependent methylation of the Ephx2 gene. Mesenteric arteries isolated from male, female, ovariectomized female (OV), and OV with estrogen replacement (OVE) mice, as well as the human cell line (HEK293T) were used. Methylation-specific PCR and bisulfite genomic sequencing analysis indicate significant increases in DNA/CG methylation in vessels of female and OVE compared with those of male and OV mice. The same increase in CG methylation was also observed in male vessels …

From The Cover: 7,8-Dihydroxyflavone Rescues Lead-Induced Impairment Of Vesicular Release: A Novel Therapeutic Approach For Lead Intoxicated Children, Xiao-Lei Zhang, Jennifer L. Mcglothan, Omid Miry, Kirstie H. Stansfield, Meredith K. Loth, Patric K. Stanton, Tomas R. Guilarte

NYMC Faculty Publications

Childhood lead (Pb2+) intoxication is a public health problem of global proportion. Lead exposure during development produces multiple effects on the central nervous system including impaired synapse formation, altered synaptic plasticity, and learning deficits. In primary hippocampal neurons in culture and hippocampal slices, Pb2+ exposure inhibits vesicular release and reduces the number of fast-releasing sites, an effect associated with Pb2+ inhibition of NMDA receptor-mediated trans-synaptic Brain-Derived Neurotrophic Factor (BDNF) signaling. The objective of this study was to determine if activation of TrkB, the cognate receptor for BDNF, would rescue Pb2+-induced impairments of vesicular release. Rats were chronically exposed to Pb2+ …

Efficacy Of Human Placental-Derived Stem Cells In Collagen Vii Knockout (Recessive Dystrophic Epidermolysis Bullosa) Animal Model, Yanling Liao, Larisa Ivanova, Rajarajeswari Sivalenka, Trevor Plumer, Hongwen Zhu, Xiaokui Zhang, Mitchell S. Cairo

NYMC Faculty Publications

Recessive dystrophic epidermolysis bullosa (RDEB) is a devastating inherited skin blistering disease caused by mutations in the COL7A1 gene that encodes type VII collagen (C7), a major structural component of anchoring fibrils at the dermal-epidermal junction (DEJ). We recently demonstrated that human cord blood-derived unrestricted somatic stem cells promote wound healing and ameliorate the blistering phenotype in a RDEB (col7a1(-/-) ) mouse model. Here, we demonstrate significant therapeutic effect of a further novel stem cell product in RDEB, that is, human placental-derived stem cells (HPDSCs), currently being used as human leukocyte antigen-independent donor cells with allogeneic umbilical cord blood stem …

The Innocence Movement And Misdemeanors, Jenny Roberts

Hofstra Law Faculty Scholarship

No abstract provided.

Asor 2017 Annual Meeting, Paul J. Ray

Faculty Publications

No abstract provided.

The Distinction Of The Interactions Between The Transmembrane Domains Of Basigin Gene Products And Monocarboxylate Transporters, Joseph D. Fong

UNF Graduate Theses and Dissertations

Although it was once thought that neurons solely rely on glucose as a substrate for cellular energy production, it is now known that small monocarboxylate molecules, like pyruvate, lactate, and ketone bodies, are also utilized. Monocarboxylates are transported across plasma membranes via facilitated diffusion using a family of transport proteins known as monocarboxylate transporters (MCTs). Four MCTs (MCT1, MCT2, MCT3, and MCT4) are expressed within neural tissues. Expression of the MCTs has been tied to co-expression of a cell adhesion molecule belonging to the Basigin subset of the immunoglobulin superfamily (IgSF). Basigin gene products are known to interact with MCT1 …

Tetratricopeptide 39c (Ttc39c) Is Upregulated During Skeletal Muscle Atrophy And Is Necessary For Muscle Cell Differentiation, Caleb Hayes

UNF Graduate Theses and Dissertations

Ttc39c has been identified as a novel gene in skeletal muscle that is upregulated in response to neurogenic atrophy in mice. Quantitative PCR and Western blot analysis confirmed that Ttc39c is expressed in both proliferating and differentiated muscle cells. Furthermore, comparison of Ttc39c expression in undifferentiated and differentiated C₂C₁₂ cells demonstrated that Ttc39c levels peak in early differentiation, but decreases as cells become fully differentiated myotubes. The transcriptional regulation of Ttc39c was examined by cloning promoter fragments of the gene and fusing it with the SEAP reporter gene. The Ttc39c reporter gene constructs were transfected into muscle …

Cross-Cultural Study Of The Predictors Of Learning In Children Ages 11-15 Years Old, Nadina Melina Williams

UNF Graduate Theses and Dissertations

Academic achievement is deemed a significant indicator for a successful future. Cognitive ability, home environment, and metacognition are among the many factors research has posited to contribute to academic achievement and later success (Alloway & Alloway, 2010; Bradley & Caldwell, 1984, Veenman, Wilhelm, & Beishuizen, 2004). The present study examines the relation between cognitive ability (working memory (WM) and nonverbal ability), metacognitive awareness, implicit theories of IQ, home environment (socioeconomic status (SES), home life, and parental involvement in homework), and learning outcomes (grades) in two distinct sample populations. The study used a sample of 11-15-year-olds and their parents from two …

Molecular And Evolutionary Analysis Of Cyanobacterial Taxonomic Methods, Chelsea Denise Villanueva

UNF Graduate Theses and Dissertations

Cyanobacteria are a group of photo-oxygenic bacteria found in nearly every ecosystem, but much cyanobacterial diversity, in various habitats, has yet to be explored. Cyanobacteria are often conspicuous components of photosynthetic flora, providing significant carbon and nitrogen inputs to surrounding systems. As possible primary colonizers of stone substrates not native to this region, cyanobacteria isolated from headstones may provide biogeographically informative data. An exploratory study of lichen-dominated microbial consortia, growing on headstones, was conducted to isolate and identify novel microaerophytic cyanobacteria, and resulted in the establishment of four novel cyanobacterial taxa. Phylogenetic analyses of photobionts in one tripartite lichen revealed …

Opioid Use Disorder: The Ugly Return And Treatment Effectiveness Of Heroin Use, Antwana L. Drayton

UNF Graduate Theses and Dissertations

Relationships among demographic, socioeconomic and person factors and Opioid Use Disorder diagnosis, treatment, and recidivism were explored. Data from a sample of 4,860 adults with substance use difficulties were analyzed. A program evaluation was conducted on Gateway Community Services to explore the use of Medication Assisted Treatments (MATs) and Psychosocial (PS) treatments to address Opioid Use Disorder. Using archival data, a chi-square analysis and independent sample t-test was performed. The results expressed that a relationship among race, type of substance use diagnosis, and treatment type and recidivism rate was found. While White/Non-Hispanics adults were more likely to use heroin than …

Characterization Of Vitellogenesis In The Bonnethead Shark Sphyrna Tiburo, Adrien Kathleen Mowle

UNF Graduate Theses and Dissertations

Vitellogenin (Vtg) is a precursor to yolk-proteins produced in the liver of many invertebrates and non-mammalian vertebrates; its synthesis is stimulated by the hormone estradiol (E2). This study is the first to characterize vitellogenin synthesis in a placental viviparous elasmobranch, the yolk-sac placental bonnethead shark, Sphyrna tiburo. This study focused on determining where and when Vtg is produced, as well as what hormonal factors regulate Vtg production. The liver was confirmed as the site of Vtg production via immunohistochemistry. Immunoreactivity was also observed within granulosa cells of ovarian follicles; further experimentation is needed to determine if this is indicative …

The Inevitability Of Decay: Disability In Ernest Hemingway's The Old Man And The Sea, Dominic Robin

UNF Graduate Theses and Dissertations

To many, Ernest Hemingway embodies a certain image of "masculinity," one centered around ability and physical performance. Such a narrative ignores the truly complicated and dynamic shape his understanding of the body took. Through an analysis of Hemingway's The Old Man and the Sea, I examine the form this ideology took in his later life, focusing particularly on Hemingway's evolved understanding of the body. Through this research, a more nuanced picture of Hemingway emerges, one that recognizes the complicated and dynamic nature his view of the "able" body took.

Establishing Relationships Among Environmental Stressors, Host Immune Status, And Wasting Disease Susceptibility In The Dominant Seagrass Species Thalassia Testudinum, Paige Joy Duffin

UNF Graduate Theses and Dissertations

A growing body of evidence supports the observation that marine disease outbreaks, especially those caused by opportunistic pathogens, are increasing in frequency and severity. One genus of such pathogens, Labyrinthula, has been identified as the causative agent of seagrass wasting disease, an epidemic that has historically plagued seagrass beds around the world. It is suspected that pathogenicity is intimately linked to the ability of the host to initiate defense responses, but a lack of compelling evidence prevents any meaningful application of preliminary observations. This body of work investigated the roles of host genotype, host immune status, and environmental stressors …

Vertical Distribution Of Meroplankton And Bivalve Competition In A Well-Mixed Estuary, Jennifer M. Raabe

UNF Graduate Theses and Dissertations

If we want to understand how meroplankton utilize the water column and how their vertical distribution may influence horizontal advection, it is important to study their behavior in the various environments where they exist. In a well-mixed system with physical cues dampened, and no vertical layering, these organisms will have to depend on environmental cues such as light, tidal current, and tide cycle, as well as their own swimming ability to migrate vertically. Plankton and water samples were collected at three depths (near surface, midwater, near bottom) during the summers of 2013 and 2014 from sites within the main channel …

Teacher Perspectives Regarding The Pedagogical Practices Most Culturally Responsive To African American Middle School Students, Robert James Mcgill

UNF Graduate Theses and Dissertations

This dissertation examines teacher’s perspectives regarding the classroom strategies,

behaviors, and approaches they believed best support the development of African American students. Educator perceptions are valuable to understand because perceptions and attitudes undergird behavior and practices. This study focused on perceptions of teachers toward pedagogical strategies, approaches, and teacher behaviors that perceived to best support African American students because of the persisting achievement gap between African American students and their White, middle class counterparts.

Culturally Relevant Pedagogy was used as the theoretical framework for this study as it describes approaches to teaching students from historically marginalized groups in ways that …

Parent Perceived Stress And Child Temperament: Qualities That Facilitate Or Impede Child Developmental Outcomes, Rebekah Faith Klempin

UNF Graduate Theses and Dissertations

Effective parent-child relationships contribute to the development of well-adjusted children. Taxing personal and situational factors encumber a caregiver’s capacity for responsivity with his or her child. The purpose of the present study was to identify interpersonal factors that impact child outcomes in low socioeconomic status family populations. Data was collected in northeast Florida Head Start centers from 219 low income, at-risk caregivers and their children ages one and a half through almost five. Parents completed questionnaires on parent perceived stress, child temperament, and child developmental outcomes. Hierarchical regression was used to assess the influence of child temperament and parent perceived …

The Efficacy Of Commercial Tooth Storage Media For Maintaining The Viability Of Human Periodontal Ligament Fibroblasts, W. Lee, Sheila E. Stover, Morteza Rasoulianboroujeni, K. Sherman, Farahnaz Fahimipour, E. Dashtimoghadam, C. Zito, Hossein E. Jazayeri, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Aim

To evaluate Save‐A‐Tooth (SAT), EMT Toothsaver (EMT) and Hank's Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs).

Methodology

Primary HPDLFs were seeded into 96‐well cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 h at room temperature (22 °C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (ATP) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24 h storage in each medium. The …

Molecular Characterization Of The Mummichog (Fundulus Heteroclitus) Ovarian Steroidogenic Pathway And Implications For Exogenous Estrogen Effects During Follicular Development, Thiviya Kanagasabesan

Theses and Dissertations (Comprehensive)

This study investigated ovarian development in Fundulus heteroclitus (mummichog), through molecular characterization of key mediators in the ovarian steroidogenic pathway; these included ovarian StAR (steroidogenic acute regulatory protein), cytochrome P450 enzymes, estrogen receptors (ERα, ERβ1 and ERβ2), and gonadotropin receptors (FSHr and LHr); additionally, hepatic vitellogenin (VTG1) and ERs, and brain P450 aromatase (P450arom; CYP19b) were studied. Fish were staged into cortical alveolus, vitellogenic, early mature, late mature, and ovulating stages of maturation; blood (plasma), ovary (tissue and isolated ovarian follicles per stage), liver, and brains were collected. Testosterone (T), 17β-estradiol (E₂) and maturation inducing steroid (MIS), measured …

The Hold Me Tight Program For Couples Becoming Parents: A Mixed Methods Study, Debbie Wang

Theses and Dissertations (Comprehensive)

ABSTRACT

Attachment theory has made substantial contributions to the understanding of close relationships. The purpose of this study was to inquire whether an attachment-informed psychoeducational program is a feasible and effective intervention for couples expecting their first child. The overarching question was: Is an attachment-informed relationship enhancement program, such as Hold Me Tight^® (HMT), helpful to couples in strengthening their relationship and increasing their confidence in becoming first-time parents? The research question was addressed using a mixed-methods approach.

In the first phase, the Hold Me Tight^® program developed by Dr. Sue Johnson was modified for use with couples …

Shame In The Presence Or Absence Of God In Stephen Ministry, Richard Tamas

Theses and Dissertations (Comprehensive)

This hermeneutical phenomenological study explores the lived experience of shame in relation to the presence or absence of God in the caregiving process of Stephen Ministry. Stephen Ministers are trained Christian caregivers who walk side-by-side with those who are struggling in a congregation. Stephen Ministers are trained by Stephen Leaders who have completed the Stephen Ministry Leadership training program. A concern for an assurance of quality of care led to the research question: what is the lived experience of unacknowledged or unprocessed shame in the presence or absence of God experienced by Stephen Ministers who offer Christian care? The foundations …

Evidence For Extra-Gastric Expression Of The Proton Pump H+/K+ -Atpase In The Gills And Kidney Of The Teleost Oreochromis Niloticus, Ebtesam Barnawi

Theses and Dissertations (Comprehensive)

It is well known that stomach acid secretion by oxynticopeptic cells of the gastric mucosa is accomplished by the H⁺/ K⁺-ATPase (HKA), which is comprised of the HKα1 (gene: atp4a) and HKβ (gene: atp4b) subunits. However, the role of the HKA in extra-gastric organs such as the gill and kidney is less clear especially in fishes. This pump may contribute to active ion and/or acid-base regulation either through direct ion transport or through secondary transport proteins against unfavorable concentration gradients via the energy derived from ATP hydrolysis. In the present work I have demonstrated …

Emerging Adults' Parent-Child Privacy Management Surrounding Topics Of Career, Religion, And Politics, Maria Butauski

Iowa Journal of Communication

Guided by communication privacy management theory (Petronio, 2002), this study highlights the role of emerging adults' perceptions of parents' behaviors in discussions of career, religion, and politics in their management of the parent-child privacy boundary. Parental behaviors perceived as supportive were associated with more openness and less topic avoidance when discussing career, religion, and politics. However, parental behaviors perceived as interference or neglect were associated with less openness and more topic avoidance across the topics addressed Findings and directions for future research are discussed

Testing The Network Reset Hypothesis: Noradrenergic Modulation Of Hippocampal Representations, Stephanie L. Grella

Theses and Dissertations (Comprehensive)

The locus coeruleus (LC) responds to salience cues, including novelty, and sends a major noradrenergic projection to the hippocampal formation (HF). Novelty-associated LC activation may help to sculpt contextual representations in the HF, but modulatory influence of norepinephrine (NE) over HF representations remains poorly understood. One possible mechanism is that NE provides a “reset” signal causing the HF to recruit distinct neural populations, thereby providing a molecular switch to dictate if hippocampal circuits should generate new representations or update existing ones to incorporate novel information. This hypothesis suggests that NE release should cause the HF to recruit a unique population …

The Role Of Channel Fens In Permafrost Degradation Induced Changes In Peatland Discharge At Scotty Creek, Nt, Lindsay Elena Stone

Theses and Dissertations (Comprehensive)

Permafrost degradation in the peat-rich southern fringe of the discontinuous permafrost zone is producing substantial changes to land cover with concomitant expansion of permafrost-free wetlands (bogs and fens) and shrinkage of forest supported by permafrost peat plateaus. Predicting discharge from headwater basins in this region depends on understanding and numerically representing the interactions between storage and discharge within and between the major land cover types, and how these interactions are changing. To better understand the implications of land cover change on wetland discharge, the hydrological behaviour of a channel fen in the headwaters of Scotty Creek, Northwest Territories, Canada, dominated …

Navigating Risks Across Borders: The Lived Experiences Of Central American Women Migrants, Carla Angulo-Pasel

Theses and Dissertations (Comprehensive)

The journey for ‘unauthorized’ migrant women from the Northern Triangle of Central America (NTCA) through Mexico dangerous and violent. In hopes of achieving safe passage to the United States (U.S.), women migrants will have to navigate borders. In this dissertation, I use the concept of borders to reveal the gendered experiences of (im)mobility. I argue that navigating borders throughout the migratory journey is not simply about experiencing the risks and vulnerabilities associated with restrictive border enforcement policies and practices implemented by the nation-state. (Im)mobility for women migrants is equally about the boundaries and/or barriers that are created by oppressive systems …

Relationships Between Factors Influencing Biofilm Formation And Pathogen Retention In Complex Rhizosphere Microbial Communities, Aaron Coristine

Theses and Dissertations (Comprehensive)

Riparian wetlands are unique habitats facilitating all forms of life. The riverbanks of these environments provide ideal conditions for bacteria, plants, and higher organisms. Of particular interest to this research was the variation in microbial community structure at high, intermediate and poor water quality impacted areas. Assessing the capabilities of plants to retain microbial pathogens was identified. Root systems and corresponding soil are ideal locations for bacterial deposition, resulting in attachment at these areas. Biofilm production in these regions is important for long-term establishment, leading to persistence and potential naturalization. Opportunistic pathogens originating from mammalian fecal matter are introduced into …