Digital Commons Network^™

Proximate Composition And In Vitro Protein Digestibility Of Extruded Aquafeeds Containing Acheta Domesticus And Hermetia Illucens Fractions, F. G. Irungu, C. M. Mutungi, A. K. Faraj, H. Affognon, S. Ekesi, D. Nakimbugwe, K. K.M. Fiaboe

All Peer-Reviewed Publications

Major protein sources for feed manufacture have become costly, and this has led to increased cost of products such as aquafeeds. This has dampening effect on fish production especially in developing countries, and has necessitated a search for alternative protein sources in processed feeds. The effects of extrusion on aquafeed blends containing fractions of adult cricket (Acheta domesticus) meal (ACM) or black soldier fly (Hermetia illucens) pre-pupae meal (BSFM) on proximate composition and in vitro protein digestibility were investigated. Extrusion resulted in higher contents of protein and nitrogen free extract, and lower contents of ether extract and crude fibre as …

Ingredients For Understanding Brain And Behavioral Evolution: Ecology, Phylogeny, And Mechanism, Stephen H. Montgomer, Adrian Currie, Dieter Lukas, Neeltje Boogert, Andrew Buskell, Fiona R. Cross, Sarah Jelbert, Shahar Avin, Rafael Mares, Ana F. Navarrete, Shuichi Shigeno, Corina J. Logan

All Peer-Reviewed Publications

Uncovering the neural correlates and evolutionary drivers of behavioral and cognitive traits has been held back by traditional perspectives on which correlations to look for-in particular, anthropocentric conceptions of cognition and coarse-grained brain measurements. We welcome our colleagues' comments on our overview of the field and their suggestions for how to move forward. Here, we counter, clarify, and extend some points, focusing on the merits of looking for the "best" predictor of cognitive ability, the sources and meaning of "noise," and the ways in which we can deduce and test meaningful conclusions from comparative analyses of complex traits.

Suitability Of Egg Hatching Conditions And Commonly Available Food Plants For Rearing The Long-Horned Grasshopper Ruspolia Differens Serville (Orthoptera: Tettigoniidae), G. Ssepuuya, C. M. Tanga, I. Yekko, F. Sengendo, C. T. Ndagire, K. K.M. Fiaboe, J. Karungi, D. Nakimbugwe

All Peer-Reviewed Publications

Efforts to promote sustainable production and processing of Ruspolia differens Serville (Orthoptera: Tettigoniidae) as a viable agribusiness model for enhancing food and nutrition security have gained momentum. However, the inexistence of rearing techniques adapted to this insect creates uncertainty regarding the effectiveness of up-scaling production. This study evaluated the effect of five temperatures (26, 28, 30, 32 and 34 °C) on egg development time, percentage hatchability and nymphal weight at hatching. It also evaluated the average weekly wet weight attained by R. differens and percentage survival during growth when reared at 30 °C on four different food plant diets. The …

Insect Net: A Novel Technology To Promote Integrated Pest Management On Horticultural Crops In Africa, T. Martin, M. Saidi, S. Niassy, S. Simon, F. Vidogbena, L. Parrot, S. Ekesi, E. Deletre, S. Subramanian, F. Assogba-Komlan, V. Baird, K. K.M. Fiaboe, M. Ngouajio, J. E. Simon, A. Ratnadass

All Peer-Reviewed Publications

Fruits and vegetables are important commodities in global agriculture. However, in sub-Saharan Africa, production is sub-optimal in terms of yield, quality and environmental health practices due to several constraints such as insect pests which reduce yields through direct damage, or indirectly as disease vectors often promoting microbial diseases. Pest control still relies mainly on chemicals with their associated risks of resistance selection and toxicity to humans and environment. Several alternatives including biopesticides, semiochemical and nets are being explored in pest management. Insect nets applied on tomatoes, cabbages, beans and leafy vegetables protect them from flies, butterflies, moths, albeit to a …

Effects Of Waste Stream Combinations From Brewing Industry On Performance Of Black Soldier Fly, Hermetia Illucens (Diptera: Stratiomyidae), Shaphan Y. Chia, Chrysantus M. Tanga, Isaac M. Osuga, Samira A. Mohamed, Fathiya M. Khamis, Daisy Salifu, Subramanian Sevgan, Komi K.M. Fiaboe, Saliou Niassy, Joop J.A. Van Loon, Marcel Dicke, Sunday Ekesi

All Peer-Reviewed Publications

Background: In recent years, there has been a rapidly growing demand for readily accessible substrates for mass production of Black Soldier Fly, Hermetia illucens Linnaeus. Beer production results in various by-products that typically end up in uncontrolled dumpsites constituting pollution problems, which merits urgent attention. The present study investigated whether the 12 formulated diets composed of brewers’ spent grains (BSGs), brewers’ yeast and cane molasses can serve as substrate for H. illucens production. Methods: Four different BSGs were selected and formulated into 12 diets, aiming at varying protein and net energy levels. The diets were offered to newly hatched (∼1 …

A Teacher Goes Gothic: Walter White, Heisenberg And The Dark Revenge Of Science, J. Patrick Mcgrail, Ewa Mcgrail, Alicja Rieger

Research, Publications & Creative Work

Much has been written on the natures and personalities of teachers in educational research and publications (Carter, 2009; Beck, 2012; Bulman, 2015; Dalton, 2013; Gillard, 2012; Kelly & Caughlan, 2011). However, mass media can have a powerful influence on how people see teachers. The television series Breaking Bad (Gilligan, 2007-2013) and its representation of teachers, has contributed a unique – if warped -perspective on the subject of teachers and teaching in America. In this article, we argue that three important Gothic and mythic fables from literature are harbingers of Walter White and his transformation – the Faust legend, as told …

Introductory Statistics, Barbara Illowsky, Susan Dean

Open Access Textbooks

Introductory Statistics follows scope and sequence requirements of a one-semester introduction to statistics course and is geared toward students majoring in fields other than math or engineering. The text assumes some knowledge of intermediate algebra and focuses on statistics application over theory. Introductory Statistics includes innovative practical applications that make the text relevant and accessible, as well as collaborative exercises, technology integration problems, and statistics labs.

Characterizing Curiosity-Related Behavior In Bottlenose (Tursiops Truncatus) And Roughtoothed (Steno Bredanensis) Dolphins, Malin Lilley, Amber J. De Vere, Deirdre Yeater, Stan A. Kuczaj Ii

Psychology Faculty Publications

Dolphins are frequently described as curious animals; however, there have been few systematic investigations of how dolphins behave when they are curious and the extent to which individual differences in curiosity exist in dolphins. Previous research has described individual differences in dolphins’ frequency of interactions with environmental enrichment as well as quantifying curiosity-related traits of dolphins via personality assessments, though behavioral observation and trait rating components have not been part of the same study. The present study describes two different experiments designed to elicit curiosity in 15 bottlenose (Tursiops truncatus) and 6 rough-toothed (Steno bredanensis) dolphins. …

Role Of Sphingosine-1-Phosphate Receptors In Cytokine And Chemokine Production By Glia, Jean Moon

Theses and Dissertations

About 1 in 10 newly diagnosed HIV cases in the United States are attributed to injection drug abuse. Opiate abuse exacerbates HIV disease progression in the central nervous system by disrupting glial function and significantly augmenting glial-derived pro-inflammatory mediators. Astroglia and microglia exposed to viral proteins, such as transactivator of transcription (Tat), become activated leading to release of a large number of cytokines and chemokines and a positive loop of neuro-inflammation. Despite effective antiretroviral therapy, persistent inflammation and immune activation affect HIV-infected individuals. A potential alternative target is sphingosine 1-phosphate receptor 1 (S1PR1) which is known to play a role …

Activation Of Trpv1 By Capsaicin Regulates Enac, Alexander Saffran

Theses and Dissertations

ENaC is a constitutively open heterotrimeric channel which regulates Na⁺ transport in tight epithelia of the kidney, lungs, colon and anterior tongue containing fungiform taste buds. The amiloride-sensitive ENaC is comprised of aβg subunits. Humans express an additional subunit, the d subunit. Therefore, humans contain both aβg-ENaC and dβg-ENaC functional channels. Relative to aβg-ENaC, the dβg-ENaC is 10-fold less sensitive to amiloride. In the mammalian anterior tongue, ENaC is expressed in fungiform salt sensing taste receptor cells and is the Na⁺-specific salt taste receptor. In mammals, salt elicits an inverted U shaped behavioral response. Lower concentrations of …

Improving The Conservation Of A Cryptic Endangered Freshwater Mussel (Parvaspina Collina) Through The Use Of Environmental Dna And Species Distribution Modeling, Bonnie A. Roderique

Theses and Dissertations

Conservation efforts that involve habitat protection, population augmentation, and species reintroductions require knowledge of the habitat requirements, distribution, and abundance of a species—information that can be challenging to acquire, especially for rare organisms with patchy distributions. In this thesis, I develop a protocol for the use of environmental DNA (eDNA) and create a Species Distribution Model for the endangered James spinymussel, Parvaspina collina (Unionidae). The results of this work show that eDNA is a robust tool for identifying species presence but not for estimating the relative abundance of populations. This study found that P. collina’s distribution is influenced by abiotic …

Motion-Induced Artifact Mitigation And Image Enhancement Strategies For Four-Dimensional Fan-Beam And Cone-Beam Computed Tomography, Matthew J. Riblett

Theses and Dissertations

Four dimensional imaging has become part of the standard of care for diagnosing and treating non-small cell lung cancer. In radiotherapy applications 4D fan-beam computed tomography (4D-CT) and 4D cone-beam computed tomography (4D-CBCT) are two advanced imaging modalities that afford clinical practitioners knowledge of the underlying kinematics and structural dynamics of diseased tissues and provide insight into the effects of regular organ motion and the nature of tissue deformation over time. While these imaging techniques can facilitate the use of more targeted radiotherapies, issues surrounding image quality and accuracy currently limit the utility of these images clinically.

The purpose of …

Use Of Surface Enhanced Raman Spectroscopy For The Detection Of Bioactive Lipids, Christopher M. Ohlhaver

Theses and Dissertations

The detection and analysis of lipids in biological matrices for clinical applications poses many challenges, but rapid and reliable detection will prove invaluable for clinical diagnosis. Herein, we report the application of drop-casted Ag nanoplatelets as surface enhanced Raman scattering (SERS) substrates for qualitative detection of 20-hydroxyeicosatetraenoic acid (20-HETE), which is a potential biomarker for diagnosis of hypertensive disorders. Biomarker peaks of 20-HETE can be reliably detected and differentiated from those of the structurally similar lipids (arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid) commonly found in human blood, even 1 pM concentrations. Additionally, one study mixed 20-HETE with three structurally …

Placental Eicosanoids And Sphingolipids In Preeclampsia, Daniel T. Reep

Theses and Dissertations

Placental dysfunction is implicated in the pathogenesis of preeclampsia. Chemical signals between the placenta and maternal circulation are a suspect cause of endothelial dysfunction and maternal hypertension. This study examined select lipid mediators of inflammation produced by the placenta. Patients were recruited from Virginia Commonwealth University’s pregnancy clinics and placentas were collected at delivery. Forty-eight-hour explant cultures of villous placental tissue were used to model lipid production. Electrospray ionization mass spectrometry was used to quantify concentrations of free lipids in the culture media. Bicinchoninic acid assays were performed to quantify protein in each culture for normalization of lipid data. After …

Identification Of Taxonomic And Functional Ichthyofaunal Zones Within The James River Basin, Virginia, Joseph L. Noel

Theses and Dissertations

Environmental gradients structure ichthyofaunal communities longitudinally along river networks via the selective filtering of species’ traits. In many instances, these environmental influences have created distinct zones of co-occurring fish species. Zonation studies have most often been conducted with taxonomic data (species x site matrices), but the increasing availability of functional trait data creates an opportunity to build more rigorous understanding of species’ co-occurrence patterns. Notably, zonation studies that use taxonomic data may not reveal the same patterns as studies based on trait data. In this study, we tested for distinct ichthyofaunal zonation in James River Basin, VA using a combination …

Investigating The Role Of Nicotinic Acetylcholine Receptor Agonists In Lung Cancer Progression And Chemosensitivity In The Context Of Treating Chemotherapy-Induced Peripheral Neuropathy, Sarah L. Kyte

Theses and Dissertations

While cancer chemotherapy continues to significantly contribute to the number of cancer survivors, exposure to these drugs can often result in chemotherapy-induced peripheral neuropathy (CIPN), a consequence of peripheral nerve fiber dysfunction or degeneration. CIPN is characterized by sensory symptoms in the hands and feet, such as numbness, burning, and allodynia, resulting in an overall decrease in quality of life. Paclitaxel (Taxol), a microtubule poison that is commonly used to treat breast, lung, and ovarian cancers, has been found to cause CIPN in 59-78% of cancer patients. There is currently no effective preventative or therapeutic treatment for this side effect, …

Association Between Warfarin Adherence Trajectories, Hospitalization Risk, And Healthcare Utilization Among Medicare Patients With Atrial Fibrillation: A Group-Based Trajectory Modelling Approach, Mai Alhazami

Theses and Dissertations

Introduction: Warfarin is the most commonly prescribed drug for stroke prevention among Atrial Fibrillation (AF) patients, especially in older adult populations, but medication nonadherence reduces its effectiveness in clinical practice. Group Based Trajectory Models (GBTM) have been used to identify distinct patterns of adherence behavior related to various medications and understand the patient characteristics associated with each trajectory. The objectives of the study were: 1) Describe trajectories of warfarin adherence among Medicare AF patients, 2) Assess impact of adherence trajectories on AF-related hospitalization, 3) Estimate the AF-related direct costs for each adherence trajectory group.

Methods: We identified elderly AF patients …

Evaluation And Adaptation Of Live-Cell Interferometry For Applications In Basic, Translational, And Clinical Research, Kevin A. Leslie

Theses and Dissertations

Cell mass is an important indicator of cell health and status. A diverse set of techniques have been developed to precisely measure the masses of single cells, with varying degrees of technical complexity and throughput. Here, the development of a non-invasive, label-free optical technique, termed Live-Cell Interferometry (LCI), is described. Several applications are presented, including an evaluation of LCI’s utility for assessing drug response heterogeneity in patient-derived melanoma lines and the measurement of CD3+ T cell kinetics during hematopoietic stem cell transplantation. The characterization of mast cells during degranulation, the measurement of viral reactivation kinetics in Kaposi’s Sarcoma, and drug …

Efficient Information Dissemination In Vehicular Networks With Privacy Protection, Xiaolu Cheng

Theses and Dissertations

Vehicular ad hoc network (VANET) is a key component of intelligent transportation System (ITS). In VANETs, vehicles and roadside units exchange information for the purpose of navigation, safe driving, entertainment and so on. The high mobility of vehicles makes efficient and private communications in VANETs a big challenge.

Improving the performance of information dissemination while protecting data privacy is studied in this research. Meet-Table based information dissemination method is first proposed, so as to improve the information dissemination, and to efficiently distribute information via utilizing roadside units, Cloud Computing, and Fog Computing. A clustering algorithm is proposed as well, to …

Colloidal Synthesis And Photophysical Characterization Of Group Iv Alloy And Group Iv-V Semiconductors: Ge1-Xsnx And Sn-P Quantum Dots, Venkatesham Tallapally

Theses and Dissertations

Nanomaterials, typically less than 100 nm size in any direction have gained noteworthy interest from scientific community owing to their significantly different and often improved physical properties compared to their bulk counterparts. Semiconductor nanoparticles (NPs) are of great interest to study their tunable optical properties, primarily as a function of size and shape. Accordingly, there has been a lot of attention paid to synthesize discrete semiconducting nanoparticles, of where Group III-V and II-VI materials have been studied extensively. In contrast, Group IV and Group IV-V based nanocrystals as earth abundant and less-non-toxic semiconductors have not been studied thoroughly. From the …

The Role Of Vegetation-Topographic Interactions In A Barrier Island System: Island Migration In A Changing Climate, Benjamin Nettleton

Theses and Dissertations

Islands have been characterized based on vegetation and topography as exhibiting different disturbance regimes - reinforcing or resisting. This study had two objectives: quantify barrier island upland migration and vegetation cover change over 32 years (1984-2016), and assess tolerance of two prevalent dune grass species, A. breviligulata, and S. patens to sand burial. Using Landsat imagery from the Virginia Coast Reserve, islands were categorized within the disturbance resistance/reinforcing framework based on dune elevation. Resistant areas were associated with woody cover and low marsh to upland migration while reinforcing areas had low vegetation cover and high rates of migration. System-wide, …

Mass Spectrometry-Based Proteomics Analysis Of Secreted Proteins, Emmanuel K. Cudjoe Jr

Theses and Dissertations

Secreted proteins play important roles in many cellular functions and molecular processes. Because secreted proteins potentially enter the blood stream, they can serve as valuable measures of health and disease useful for disease diagnosis and prognosis, therapeutic target identification, and patient stratification in personalized medicine. Consequently, significant interest exists in secreted protein analysis within complex biospecimens, particularly blood but significant bioanalytical challenges including the wide protein dynamic range >10 orders of magnitude remain. The cellular secretome therefore represents a viable alternative to direct biomarker discovery in biofluids. Finally, cellular systems are amenable to labeling for the production of intact stable …

Housing Status, Patient Characteristics, And Ed Utilization Associated With Medication Prescribing At Ed Discharge Among Homeless And Nonhomeless Adults In Urban Hospitals In The United States, Lauren Cox

Theses and Dissertations

This cross-sectional study used a weighted sample of ED visits contained in the 2010-2015 years of the National Hospital Ambulatory Care Survey-Emergency Department (NHAMCS-ED) dataset. The purpose of this study was to: 1) identify differences in predisposing, enabling, and need characteristics, and ED use and medication prescribing characteristics between homeless and nonhomeless ED users; 2) assess the association between housing status and medication prescribing at ED discharge, and identify variables contributing to the disparity in medication prescribing between homeless and nonhomeless ED users; and 3) assess the predisposing, enabling, need, and ED use characteristics that predict medication prescribing at ED …

Integral Measurement Of Atmospheric Particulate Matter (Pm), Di Liu

Theses and Dissertations

Atmospheric aerosol particles also known as atmospheric particulate matter or particulate matter (PM) are microscopic particles (solid or liquid) suspended in air, which is one of six air pollutants in US air quality standard. PM is classified as coarse particles with diameters between 2.5 to 10 mm, fine particles with a diameter less than 2.5 mm (PM_2.5), and ultrafine particles with the diameter less than 0.1 mm (PM_0.1). Epidemiological studies have already showed the adverse health effects (such as asthma, lung cancer and respiratory and cardiovascular disease) resulted from exposure to the fine and ultrafine particles. …

Role Of C121a In Mglur2 Homodimeric Expression And Function, Jong M. Shin

Theses and Dissertations

The group II metabotropic glutamate receptors are known for their involvement in various psychiatric disorders. The mGluR2 in particular is linked with etiology of schizophrenia especially in the context of crosstalk with 5-HT2A. Thus, the mGluR2 has attracted attentions for its potential therapeutic applications. Despite numerous physiological evidences on the actions of mGluR2, its mechanism is still unclear to this day. It is partially due to the lack of understanding in characteristics of mGluR2 homodimer which is its functionally active form. Therefore, the characterization of dimeric interaction serves as a foundation to advanced understanding of the role of mGluR2. On …

In Vitro Genetic Code Expansion And Selected Applications, Emil S. Iqbal

Theses and Dissertations

The ability of incorporation non-canonical amino acids (ncAAs) using translation offers researchers the ability of extend the functionality of proteins and peptides for many applications including synthetic biology, biophysical and structural studies, and discovery of novel ligands. Here we describe the three projects where the addition of ncAAs to in vitro translation systems creates useful chemical biology techniques. In the first, a fluorinated histidine derivative is used to create a novel affinity tag that allows for the selective purification of peptides from a complex mixture of proteins. In the second, the high promiscuity of an editing-deficient valine-tRNA synthetase (ValRS T222P) …

Investigating Racial Bias In Perceptions Of Free Will, Courtney J. Alderson

Theses and Dissertations

The overarching goal of this study was to examine whether perceptions of others’ free will would differ depending on perceiver race as well as target race. The current study proposed that such a racial bias may be one mechanism by which racial disparities in medical treatment recommendations arise. By bridging findings from four different lines of research (i.e., the literatures on racial health and medical treatment disparities, racial bias, free will beliefs, and social identity), it was hypothesized that: (1) participants would perceive greater amounts of free will for a hypothetical racial ingroup patient than an outgroup patient; (2) such …

An Investigation Of Electronic Cigarette Aerosol Mixture On Craniofacial Development In Mouse Embryos, Suraj Kandalam

Theses and Dissertations

E-cigarette (e-cig) use has increased in recent years among teenagers and young adults. E-cigs are battery-powered nicotine delivery systems that vaporize e-liquids, a solution composed of propylene glycol (PG), vegetable glycerin (VG), nicotine, flavoring, and coloring agents. The aim of this study was to determine the effects of exposure to e-cig aerosol mixtures (e-cigAMs) on proliferation, migration, and differentiation of NCCs in vitro and craniofacial development in mouse embryos in vivo. E-cigAM was produced by bubbling e-cig aerosol into saline. E-cigAMs were generated for unflavored e-liquid of increasing PG: VG ratio. Metabolic activity (MA), gene expression, migration, and proliferation …

Exploring Literacies In The Assemblage Of Adult Education English For Speakers Of Other Languages Classrooms, Susan Watson

Theses and Dissertations

The purpose of this dissertation is to provide a posthuman perspective of adult second language and literacy learning using the philosophy of Gilles Deleuze and his collaborative work with Félix Guattari, Masny’s (2005/6) multiple literacies theory or MLT, and DeLanda’s (2016) assemblage theory. Thinking with these scholars, I employ a post-qualitative, posthuman MLT conceptual framework to study literacy as a process that flows through and connects with globally-diverse students, languages, worldviews, and texts in the assemblage of adult education, English for speakers of other languages (ESOL) classrooms. I posit this assemblage as a remarkable and important context for literacy research …

Novel Insights Into The Contribution Of Cellular Senescence To Cancer Therapy: Reversibility, Dormancy And Senolysis., Tareq Saleh

Theses and Dissertations

Cellular senescence a specialized form of growth arrest that contributes to the pathogenesis of several aging-related disorders including cancer. While by definition tumor cells are considered immortalized, they can undergo senescence when exposed to conventional and targeted cancer therapy. Therapy-Induced Senescence (TIS) represents a fundamental response to therapy and impacts its outcomes. However, TIS has been considered a positive therapeutic goal since senescent tumor cells are expected to enter a state of permanent growth abrogation. In this work we examined the hypothesis that a subpopulation of senescent cells can re-acquire proliferative potential after a state of senescent dormancy, indicating that …