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Pcb Disruption Of Gut And Host Health: Implications Of Prebiotic Nutritional Intervention, Jessie Baldwin Hoffman

Theses and Dissertations--Pharmacology and Nutritional Sciences

Exposure to environmental pollutants poses numerous risk factors for human health, including increasing incidence of cardiovascular disease and diabetes. Persistent organic pollutants, such as polychlorinated biphenyls (PCBs) have been strongly linked to the development of these chronic inflammatory diseases and the primary route of exposure is through consumption of contaminated food products. Thus, the gastrointestinal tract is susceptible to the greatest levels of these pollutants and is an important facet to study.

The first two hypotheses of this dissertation tested that exposure to PCBs disrupts gut microbiota directly (in vitro) and within a whole body system. PCB exposure …

Role Of Sex Chromosomes In Sexual Dimorphism Of Angii-Induced Abdominal Aortic Aneurysms, Yasir Alsiraj

Theses and Dissertations--Pharmacology and Nutritional Sciences

Abdominal aortic aneurysms (AAAs), a permanent dilation in the abdominal region of the aorta, is a highly sexually dimorphic disease. AAAs prevalence is ranging from 4-10 fold higher in males than females. Defining the mechanistic basis for reduced (in females) or increased (in males) AAA formation and progression may uncover potential therapeutic targets. The majority of studies examining sexual dimorphism focus on the role of sex hormones. However, genes residing on sex chromosomes, in addition to sex hormones, may contribute to sexual dimorphism of AAAs. For example, the X chromosome contains about 5% of the whole genome, but the role …

Β-Catenin Regulation Of Adult Skeletal Muscle Plasticity, Yuan Wen

Theses and Dissertations--Physiology

Adult skeletal muscle is highly plastic and responds readily to environmental stimuli. One of the most commonly utilized methods to study skeletal muscle adaptations is immunofluorescence microscopy. By analyzing images of adult muscle cells, also known as myofibers, one can quantify changes in skeletal muscle structure and function (e.g. hypertrophy and fiber type). Skeletal muscle samples are typically cut in transverse or cross sections, and antibodies against sarcolemmal or basal lamina proteins are used to label the myofiber boundaries.

The quantification of hundreds to thousands of myofibers per sample is accomplished either manually or semi-automatically using generalized pathology software, and …

A Comparative Study Of Aesthetic And Performance Properties Of Womens T-Shirts Made Of Lyocell, Organic Cotton, And Viscose From Bamboo, Katelyn Eubank

Theses and Dissertations--Retailing and Tourism Management

The purpose of this research was to evaluate and compare the performance, aesthetic and material properties of women’s t-shirts composed of three different sustainable fibers (lyocell, organic cotton, and viscose from bamboo) before and after repeated home laundering. A convenience sample was comprised of thirty-nine t-shirts. Thirteen black t-shirts from each fiber content were used for testing according to ASTM and AATCC standards. The evaluations and measurements were performed initially and after wash intervals one, five, ten, and twenty. The t-shirts were tested for fabric weight, fabric count, color fastness, smoothness appearance, pilling, dimensional stability and skewness.

Evaluation Of Trichoderma Spp. As Biocontrol Agents For Soybean Diseases, Jonathan Vance Lacey

Theses and Dissertations--Plant Pathology

Fungi in the genus Trichoderma have been characterized as biocontrol agents of plant pathogens since the 1930s. The use of biologicals for disease management has increased in recent years, typically marketed as a safer alternative to chemical applications. However, biologicals often lack consistent control across varying environmental conditions. To overcome the loss in efficacy due to environmental conditions, biologicals can be combined with common fungicide seed-treatments to provide improved control. Additionally, the presence of a biological organism could slow the development of a pathogen population. Greenhouse trials were conducted to determine the baseline root colonization of three Trichoderma spp. used …

Characterization And Evaluation Of Androgen-Binding Protein, Sex Hormone-Binding Globulin, And Thyroxine-Binding Globulin In The Horse, Blaire O'Neil Fleming

Theses and Dissertations--Veterinary Science

The objectives of this study are to characterize two carrier proteins in the horse that significantly decrease in humans following anabolic androgenic steroid administration: sex hormone-binding globulin (SHBG) and thyroxine-binding globulin (TBG). For SHBG characterization, qPCR, RNA sequencing, and immunohistochemistry were performed on testes and equine livers. Free and total testosterone immunoassays were utilized to confirm the presence of a carrier protein in equine circulation. SHBG was detected in the testes using qPCR, RNA sequencing, and IHC, indicating the presence of the isoform androgen-binding protein (ABP). SHBG was not detected in any liver samples. Evidence of a carrier protein was …

Detectability And Occupancy Of The Common Raven In Cliff Habitat Of Central Appalachia And Southeastern Kentucky, Joshua Michael Felch

Theses and Dissertations--Forestry and Natural Resources

Nearly extirpated from the Central Appalachians, USA by the mid-1900s as a result of human persecution, loss of forests, and absence of large mammal carrion, remnant populations of common ravens (Corvus corax) have recolonized portions of their historical range. One such area of recolonization is southeastern Kentucky where the species is listed as state threatened. Southeastern Kentucky appears to have extensive suitable breeding habitat, but raven records remain relatively rare with sightings and a few nests being confirmed during the past three decades. Because little is known about local ecology or population status of this reclusive corvid in …

Diet Composition Explains Reductions In Stream Salamander Occupancy And Abundance Along A Conductivity Gradient, Jacob Matthew Hutton

Theses and Dissertations--Forestry and Natural Resources

Changes in land use such as mountaintop removal mining with valley fills (MTR/VF) affect chemical, physical, and hydrological properties of headwater streams. Although numerous stream taxa have experienced significant declines from MTR/VF, stream salamanders appear to be particularly sensitive. Yet, the specific mechanism(s) responsible for the population declines has eluded researchers. We sampled salamander assemblages across a continuous specific conductivity (SC) gradient in southeastern Kentucky and estimated occupancy rates and abundance estimates along this gradient. We also examined the diet of larval and adult salamanders to determine if autochthony (A/T prey), total prey volume, and body condition is influenced by …

Efficacy Of Herbivore Exclusion On Planted Tree Seedling Vitality On A Reclaimed Surface Mine In Eastern Kentucky, Zachary J. Hackworth

Theses and Dissertations--Forestry and Natural Resources

Conventional Appalachian surface-mine reclamation techniques repress natural forest regeneration, and tree plantings are often necessary for reforestation. Reclaimed Appalachian surface mines harbor a suite of mammal herbivores that forage on recently planted seedlings. Anecdotal reports across Appalachia have implicated herbivory in the hindrance and failure of reforestation efforts, yet empirical evaluation of herbivory impacts on planted seedling vitality in this region remains relatively uninitiated. First growing-season survival, height growth, and mammal herbivory damage of black locust (Robinia pseudoacacia L.), shortleaf pine (Pinus echinata Mill.), and white oak (Quercus alba L.) are presented in response to varying intensities …

Initial Assessment And Effects Of Snake Fungal Disease On Populations Of Snakes In Kentucky, Jennifer Mckenzie

Theses and Dissertations--Forestry and Natural Resources

Pathogenic fungi are increasingly associated with epidemics in wildlife populations and represent a significant threat to global biodiversity. Snake fungal disease is an emerging disease caused by the fungus, Ophidiomyces ophiodiicola, and appears to be widespread in the eastern United States. Yet an evaluation of field diagnostics, and an understanding of the population-level consequences of the disease, are lacking. First, I evaluated the use of clinical signs to predict the presence of O. ophiodiicola across season and snake habitat affiliation (aquatic or terrestrial) and I compared two sampling methods to see if collection method impacts PCR result. Overall, snakes with …

Diet And Space Use Of The Martial Eagle (Polemaetus Bellicosus) In The Maasai Mara Region Of Kenya, Richard Stratton Hatfield

Theses and Dissertations--Forestry and Natural Resources

The martial eagle (Polemaetus bellicosus) is a vulnerable species that is declining throughout large portions of its range. There is an urgent need to improve understanding of this species’ ecology to inform its conservation. I equipped 20 adult martial eagles with global positioning system backpack transmitters to characterize diet and space use of the species in the Maasai Mara region of Kenya. The resulting high-resolution transmitter data sets allowed for the rapid location of kills and provided a means to estimate home range size. From November 2016 to April 2018, 191 kills were identified from 206 kill location …

Identifying Mechanisms Of Host Plant Specialization In Aphis Craccivora And Its Bacterial Symbionts, Thorsten Hansen

Theses and Dissertations--Entomology

Many insects form close relationships with microbial symbionts. Insect symbionts can provide novel phenotypes to their hosts, including influencing dietary breadth. In the polyphagous cowpea aphid, Aphis craccivora, the facultative symbiont Arsenophonus improves aphid performance on one host plant (locust), but decreases performance on other plants. The goal of my thesis was to investigate the mechanism by which Arsenophonus facilitates use of locust. First, I assembled an Aphis craccivora-Arsenophonus-Buchnera reference transcriptome to conduct RNAseq analysis, comparing gene expression in aphids feeding on locust and fava, with and without Arsenophonus infection. Overall, few transcripts were differentially …

Impact Of Selected Integrated Pest Management Techniques On Arthropods In Cucurbit Production Systems, Amanda R. Skidmore

Theses and Dissertations--Entomology

Cucurbits (i.e. squash, melons, pumpkins, gourds) are high value crops of global importance. Insect pests in these systems are often controlled by chemical insecticides, which are not always effective and can be damaging to the environment. Many integrated pest management (IPM) techniques have been developed for the control of pests in these systems, with a goal of improving system stability and reducing chemical inputs. The overarching goal of my research was to investigate the impact of select IPM techniques on arthropod populations and yield in organic and conventional cucurbit systems.

This dissertation can be divided into three major projects which …

The Application Of Spectral And Cross-Spectral Analysis To Social Sciences Data, Matthew James Vowels

Theses and Dissertations--Family Sciences

The primary goal of this paper is to demonstrate the application of a relatively esoteric and interdisciplinary technique, called spectral analysis, to dyadic social sciences data. Spectral analysis is an analytical and statistical technique, commonly used in engineering, that allows times series data to be analyzed for the presence of significant regular/periodic fluctuations/oscillations. These periodic fluctuations are reflected in the frequency domain as amplitude or energy peaks at certain frequencies. Furthermore, a Magnitude Squared Coherence analysis may be used to interrogate more than one time series concurrently in order to establish the degree of frequency domain correlation between the two …

“Dad, Do You Want To Play With Me?” The Impact Of Fathers Who Make Time For Play, Michael S. Sitton

Theses and Dissertations--Family Sciences

With an increase in the pace of life in the United States, there comes a recognition of the importance of prioritizing time, especially for fathers. Of the two-thirds of children who live with their father, only a percentage of them have fathers who report regular play time with their children. However, literature in the field does not explain specifically whether or not this play between father and child influences the child’s later risk taking behaviors in high school. Using data from the 2003 Fragile Families and Child Wellbeing Study (FFCWS), waves 3, 4, and 6, this quantitative study sought to …

The Mechanism Of Rna Interference In Arthropods, June-Sun Yoon

Theses and Dissertations--Entomology

RNA interference (RNAi) is a useful reverse genetics tool for investigation of gene function as well as for practical applications in many fields including medicine and agriculture. Due to the variability in RNAi efficiency, RNAi-based methods are currently being developed for controlling only coleopteran insects which are known to be amenable to RNAi. The first chapter of my thesis includes findings from research to investigate what are the factors that make coleopteran insects relatively more efficient in RNAi. I used Colorado potato beetle (CPB), Leptinotarsa decemlineata and its cell line (Lepd-SL1) as study models to identify genes that play key …

Bee Conservation In Urban Landscapes: Assessing Bee Assemblages, Bee–Attractiveness, And Nutritritional Value Of Woody Landscape Plants And Mitigating Potential Bee Hazard From Neonicotinoid Insecticides, Bernadette Maria Mach

Theses and Dissertations--Entomology

Public awareness of declining pollinator populations has increased interest in creating “bee–friendly” urban landscapes. I quantified bee visitation and assemblages of 72 species of flowering woody plants common in urban landscapes. I found strong plant species effects and variation in seasonal activity of particular bee taxa but no overall differences in bee visitation or genus diversity between native versus nonnative species or trees versus shrubs. Analysis of pollen from a subset of these plants revealed small but statistically significant differences in total and essential amino acids between native and nonnative species and trees and shrubs, although each group had species …

A Genetically-Informed Study Of The Predictors And The Development Of Delinquency, Albert J. Ksinan

Theses and Dissertations--Family Sciences

Although the rates of delinquent behavior have been decreasing since the 1990s, adolescent delinquent behavior continues to take a great toll on society as well as on perpetrators themselves. In this way, it is essential to understand the process of delinquency development. The current dissertation is comprised of three studies that analyzed the predictors and the development of delinquency using genetically-informed designs. The sample used for all studies comes from the Add Health dataset, a nationally-representative data on adolescents followed across 14 years.

The first study modeled the longitudinal development of delinquency in three adolescent cohorts: early, middle, and late …

Identifying Perceived Risks To Environmental Pollutants And Needs For Risk Communication In A Rural Appalachian Community, Elizabeth H. Travis

Theses and Dissertations--Nutrition and Food Systems

The goal of this study is to determine issues rural Appalachian residents consider most important, their perceived environmental health risk, and how community engagement can potentially improve those issues. The University of Kentucky Superfund Research Center held the Appalachian Community Health and Well-being Forum at the Letcher County Cooperative Extension Office in Eastern Kentucky. A four-member panel consisted of two local health officials, a nutrition expert, and a federal scientist; answered questions from community members. The expert panel and audience members shared concerns, success stories, and highlighted efforts to promote health in the region. Community members completed a questionnaire collecting …

Longitudinal Associations Between Sleep Functioning And Adolescent Adjustment, Gabriela Ksinan Jiskrova

Theses and Dissertations--Family Sciences

The current dissertation consists of three interrelated studies examining the relationship between sleep functioning and adolescent adjustment. Although links between sleep patterns and internalizing problems and externalizing or problem behaviors in children and adolescents have been established in literature, several gaps remain in this research. This dissertation addressed these by: (a) testing sleep problems, quantity, and chronotype in childhood as predictors of internalizing problems in adolescence (Study 1), (b) testing sleep problems, quantity, and chronotype in childhood as predictors of problem behaviors in adolescence (Study 2), and (c) testing sleep problems and quantity as mediators of the chronotype-adjustment link (Study …

Pathways From Religiosity To Couple’S Satisfaction Through Relational Virtues And Equality In Two Cultures, Ilya A. Okhotnikov

Theses and Dissertations--Family Sciences

To explore relational processes of couple’s satisfaction this study drew on the relational spirituality framework (Mahoney, 2010) in order to test a relational religiosity model to evaluate the effects of public, private, ideological, intellectual, and experiential religiosity that were mediated by relational virtues of commitment, sacrifice, forgiveness, and sanctification and relational equality on couple’s satisfaction in two cultures. Data for this component used convenience samples of English-speaking respondents (hereafter American sample; n = 1,529) and Russian-speaking respondents (hereafter Russian sample; n = 529). Results provided evidence to partially support relational religiosity model; specifically commitment, while a statistically significant intervening element, …

Trauma Training In Educational Settings: Developing A Universal Approach Training Manual, Kerri A. Flatau

PCOM Psychology Dissertations

Children who have experienced trauma(s) may display a wide variety of symptoms, including withdrawal, behavioral challenges, difficulty with focus, learning disabilities, and social/emotional delays (Cole et al., 2005). Each of these challenges can present a barrier to learning. Therefore, in order to provide the highest quality support in the school setting, teachers and other school staff need to be educated and trained on the topic of trauma. Further, they need strategies and tools regarding how to best work with all of their students, including those who have, or may have, experienced trauma. The current study examined the available research, as …

Tracing, Security, And Safety: The Problem With Wild-Caught Seafood Supply Chains, Stephen Lemay, Dave Mcmahon, Jeffrey Periatt

AMTP Proceedings 2018

This article explores the difficulties associated with long, complex, supply chains in seafood markets. It does a brief literature review, discusses some of the issues raised by the long supply chain, and then compares one long seafood supply chain to a short, problematic supply chain.

Design And Development Of A Novel Acoustic Rain Sensor With Automated Telemetry, Maria Leonora Guico, Gemalyn Abrajano, Prince Aldrin Domer, Jose Paulo Talusan

Electronics, Computer, and Communications Engineering Faculty Publications

This paper presents the recent results of the design of a novel acoustic rainfall sensing system that is low-cost, portable, and easily deployable, which makes use of the recorded sound produced by the impact of the raindrops on the sensor surface. The sensor design allows the gathering of acoustic signal power and sending it to a server after a specified time interval, either through SMS or mobile internet connection. It exists in a weather-proof, standard-conformant, standalone system with its own power supply and telemetric capabilities. These acoustic point sensors can gather rainfall data at high spatial and temporal resolutions. Such …

Public Health And Sterilization Of The Mentally Disabled: Under What Circumstances Should It Be Scrutinized Versus Granted By Court Order?, Devin Griffin

Student Works

No abstract provided.

Water Toxicity Effects Test: Survival And Growth (Dry Mass), Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Sediment Toxicity Effects Test, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Wetted Width, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Within-Stream Variability Data, Kevin W. Custer, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.

Water Toxicity Effects Test: Physicochemical Data, Kevin W. Custer, Eric B. Borth, Julia I. Chapman, Ryan W. Mcewan

Data Files

No abstract provided.