Digital Commons Network^™

Investigating The Viral Ecology Of Global Bee Communities With High-Throughput Metagenomics, David A. Galbraith, Zachary L. Fuller, Allyson M. Ray, Axel Brockmann, Maryann Frazier, Mary W. Gikungu, J. Francisco Iturralde Martinez, Karen M. Kapheim, Jeffrey T. Kerby, Sarah D. Kocher, Oleksiy Losyev, Elliud Muli, Harland M. Patch, Cristina Rosa, Joyce M. Sakamoto, Scott Stanley, Anthony D. Vaudo, Christina M. Grozinger

All Peer-Reviewed Publications

Bee viral ecology is a fascinating emerging area of research: viruses exert a range of effects on their hosts, exacerbate impacts of other environmental stressors, and, importantly, are readily shared across multiple bee species in a community. However, our understanding of bee viral communities is limited, as it is primarily derived from studies of North American and European Apis mellifera populations. Here, we examined viruses in populations of A. mellifera and 11 other bee species from 9 countries, across 4 continents and Oceania. We developed a novel pipeline to rapidly and inexpensively screen for bee viruses. This pipeline includes purification …

A Larger Table: Fostering Inclusion In Museums Through Hiring Directors Of Community Engagement, Hannah M. Page

Museum Studies Theses

Museums have undergone a transformation over the course of their existence, shifting from inwardly-focused private collections to organizations working to represent and advocate for the diversity of human existence. While professionals in the field have become increasingly aware of the importance of expanding beyond traditional audiences, there is little consensus about the best methods of achieving this goal. This thesis proposes one potential solution, the creation of a staff position dedicated to engaging members of the museum’s surrounding community through relationship-building. After a brief exploration of the history of the museum field, this paper delves into the benefits of developing …

Portraits With A Posthumous Voice: Reinforcing And Contesting Social Norms In The Heterotopic Museum And Cemetery, Matthew J. Crissey

Museum Studies Theses

Abstract

The following paper qualitatively analyzes and documents over 500 memorial-photographs/etched portraits on tombstones in ten Western New York cemeteries. This paper covers fourteen topics, ranging from religion to gang-violence. A juxtaposition of portraits exhibited within the Smithsonian National Portrait Gallery with memorial-portraits on tombstones revealed heterotopic environments creating a public forum enabling the reinforcing or contestation of social ideologies. In other words, the author observed the similarities of identities and social norms publicly expressed on tombstones and gallery portraits.

A Social Constructionist approach enabled the study to examine how one social phenomenon contributes to the shaping of a culture. …

The Cytotoxic Effects Of Essential Oils On Estrogen Receptor Positive Mcf-7 Breast Cancer Cell Line, Desalyn Louise Johnson

Perpetua: The Journal of Undergraduate Research at UAH

The estrogen receptor positive MCF-7 breast cancer cell line was exposed to 11 essential oils that were extracted from various plants. MTT assays were executed to determine the percentage of killed cells after essential oil exposure. Three oils possessed cytotoxic activity; they originated from the species Helichrysum italicum (HEITEO), Juniperus communis (JUCOEO), and Picea mariana (PIMAEO). The acronyms are derived from the first two letters of the genus and species name, followed by "EO". HEITEO, JUCOEO, and PIMAEO produced percent kill values of 99.1%, 99.4% and 98.9% respectively at concentrations of 0.01 micrograms/microliter. An LC50 value was obtained for Picea …

Perpetua, Volume 3, Issue 1, Fall 2018

Perpetua: The Journal of Undergraduate Research at UAH

No abstract provided.

Front Matter

Perpetua: The Journal of Undergraduate Research at UAH

No abstract provided.

Mechanisms Of S1p-Induced Endothelial Barrier Enhancement, Natascha Guimarães Alves

USF Tampa Graduate Theses and Dissertations

Excessive microvascular permeability is a serious complication involved in traumatic injury and inflammatory diseases. Alcohol intoxication can exacerbate the physiological derangements produced by microvascular endothelial barrier dysfunction in such disease conditions. Sphingosine-1-phosphate (S1P) has known endothelial barrier-protective properties, and has been shown to ameliorate microvascular leakage in a model of combined alcohol intoxication and hemorrhagic shock and resuscitation (HSR). However, whether the barrier-protective properties of S1P extend to endothelial cells of the blood-brain barrier (BBB) is unclear. The mechanisms of S1P-induced barrier protection during alcohol intoxication or HSR are also unknown. In the current study, we tested the hypothesis that …

Automated Quantification And Evaluation Of Motion Artifact On Coronary Ct Angiography Images, Hongfeng Ma, Eric Gros, Scott G. Baginski, Zachary R. Laste, Naveen M. Kulkarni, Darin Okerlund, Taly Gilat Schmidt

Biomedical Engineering Faculty Research and Publications

Abstract

Purpose

This study developed and validated a Motion Artifact Quantification algorithm to automatically quantify the severity of motion artifacts on coronary computed tomography angiography (CCTA) images. The algorithm was then used to develop a Motion IQ Decision method to automatically identify whether a CCTA dataset is of sufficient diagnostic image quality or requires further correction.

Method

The developed Motion Artifact Quantification algorithm includes steps to identify the right coronary artery (RCA) regions of interest (ROIs), segment vessel and shading artifacts, and to calculate the motion artifact score (MAS) metric. The segmentation algorithms were verified against ground‐truth manual segmentations. The …

Numerical Simulation And Optimization Of Blalock-Taussig Shunt, Thomas Hess, Ramesh K. Agarwal

McKelvey School of Engineering Graduate Student Theses & Dissertations

The goal of this study is to create an optimized Blalock-Taussig shunt used to temporarily repair pulmonary vascular blockages allowing a child time to grow so a more permanent surgical repair of the heart and vasculature can be performed. Blalock-Taussig or BT shunts are a surgical procedure performed on infants suffering from cyanosis or “Blue Baby Syndrome.” A BT shunt is an artificial vessel placed between the right ventricle and the pulmonary artery to increase blood flow in the lung and blood oxygen saturation levels. In a study of 96 patients with currently in use modified BT shunts, 32 patients …

Acetylation Profiles Of Histone And Non-Histone Proteins In Breast Cancer, Alla Karpova

McKelvey School of Engineering Graduate Student Theses & Dissertations

This study evaluates the impact of protein acetylation on breast cancer gene expression and the regulation of metabolism. Acetylation is the second abundant post-translational modification after phosphorylation, regulating protein activity and function. The alterations in acetylation of both histone and non-histone proteins is known to be related to many human diseases, including cancer. Acetylation and deacetylation of histones is closely associated with the regulation of gene expression, while acetylation of non-histone proteins may have a broad effect on major cellular processes, such as proliferation, metabolism, cell cycle and apoptosis, imbalanced regulation of which is essential for cancer development. Therefore, it’s …

The Depravity Of The 1930s And The Modern Administrative State, Steven G. Calabresi, Gary Lawson

Faculty Scholarship

Gillian Metzger’s 2017 Harvard Law Review foreword, entitled 1930s Redux: The Administrative State Under Siege, is a paean to the modern administrative state, with its massive subdelegations of legislative and judicial power to so-called “expert” bureaucrats, who are layered well out of reach of electoral accountability yet do not have the constitutional status of Article III judges. We disagree with this celebration of technocratic government on just about every level, but this Article focuses on two relatively narrow points.

First, responding more to implicit assumptions that pervade modern discourse than specifically to Professor Metzger’s analysis, we challenge the normally unchallenged …

Rack1 Facilitates Efficient Translation Of Viral And Cellular Iress, Natasha Permaul

Anthropology

Ribosomes, the cellular machinery that translates mRNA sequences into protein sequences, are surprisingly heterogeneous molecules. More and more ribosomal proteins have been shown to facilitate translation of mRNA subsets. These mRNA subsets include mRNAs that can initiate translation using non-canonical pathways, for example using an internal ribosome entry site (IRES). IRESs are RNA structures that facilitate translation with fewer translation initiation factors than are required for canonical cap-dependent translation initiation. The ribosomal protein Receptor for Activated C Kinase 1 (RACK1) has been previously shown to be required for translation of the hepatitis C virus (HCV) IRES, but not required for …

A Qualitative Exploration Of The Factors That Led Hispanic/Latina Middle School Students To Select A Stem High School Education In New York City, Maria Lopez

Education Doctoral

There is a significant gender gap in science, technology, engineering, and mathematics (STEM) careers as well as in the STEM workforce that continues to expand, ultimately impacting the global economy. In response to this crisis, this qualitative study sought to identify the factors that led Hispanic/Latina middle school students to select a STEM high school education. For this study, a purposeful sample of twenty-five ninth-grade Hispanic/Latina students participated from two designated STEM private high schools located in a densely populated Hispanic/Latino area in the Bronx. Participants anonymously responded to The Middle to High School STEM Experience questionnaire (see Appendix A …

The Sky’S The Limit: Factors That Affect Females’ Decisions To Become Professional Pilots, Debra Henneberry

Education Doctoral

Despite evidence of males and females having generally comparable abilities as pilots, very few women choose this career path. According to the Federal Aviation Administration database of aviators, approximately 7% of the total pilot population in the United States is female, and 5% of professional pilot ratings are held by women. These statistics have been consistent for several decades. The purpose of this qualitative phenomenological study was to gain a better understanding of why females decide to become professional pilots. Semi-structured interviews were conducted with 12 women who, at the time of this study, were employed or had been previously …

Development And Application Of A Reverse Genetics System For Zika Virus, Jordan C. Frank

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Zika virus (ZIKV) has emerged in many regions of the world, with infection outcomes spanning from no apparent illness to crippling nervous system disease. ZIKV and its close relatives, West Nile virus, Japanese encephalitis virus, dengue virus, and yellow fever virus are primarily transmitted by mosquitoes. Three ZIKVs were selected: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015), whose place of origin and time of isolation differ substantially. Stable, complementary DNA (cDNA) copies of the three ZIKV RNA genomes were cloned to examine the significance of viral and host genetic variations in directing ZIKV infection outcomes. Using …

Developing Ultra-High Throughput Sequencing Based Assay For Ligase Ribozymes For The Study Of Evolutionary Innovations, James Collet

Boise State University Theses and Dissertations

The study of evolutionary innovations, or novel traits, is integral to understanding evolution yet is poorly understood. By studying the regions between genotype networks that produce the same phenotype, we can better characterize the process by which innovation occurs. The overarching goal of this study is to assign fitness values to the overlapping genotype network of two catalytic RNA molecules, or ribozymes. Properly characterizing this region requires the study of thousands of individual sequences, which is achievable through the use of high-throughput sequencing analysis. This thesis focuses on developing assays for one of the ribozymes, the ligase ribozyme. Due to …

Microenvironmental Forces Regulate Notch Signaling Through Integrins, Michael Allen Detweiler

Boise State University Theses and Dissertations

The extracellular microenvironment contributes significantly to a cell’s function and behavior. For instance, cell-cell interactions, cell-substrate interactions, and physical forces are all factors of the extracellular environment that can alter cellular behavior. Cells can receive these signals and forces through various membrane channels and receptors that transmit the signals from the extracellular to the intracellular space. Canonical Notch signaling is induced by ligand interactions with neighboring cells, but recent evidence has revealed that Notch signaling can occur through a variety of extracellular stimuli including hyperglycemia, hypoxia, multiple growth factors, fluid shear stress, and extracellular matrix (ECM) composition. Although Notch activation …

Privacy-Preserving Genomic Data Publishing Via Differential Privacy, Tanya Khatri

Boise State University Theses and Dissertations

Privacy-preserving data publishing is a mechanism for sharing data while ensuring the privacy of individuals is preserved in the published data and utility is maintained for data mining and analysis. There is a huge need for sharing genomic data to advance medical and health research. However, since genomic data is highly sensitive and the ultimate identifier, it is a big challenge to publish genomic data while protecting the privacy of individuals in the data.

In this thesis, we address the aforementioned challenge by presenting an approach for privacy-preserving genomic data publishing via differentially-private suffix tree. The proposed algorithm uses a …

A Novel Fpga Implementation Of Hierarchical Temporal Memory Spatial Pooler, Paul Jeffrey Mitchell

Boise State University Theses and Dissertations

There is currently a strong focus across the technological landscape to create machines capable of performing complex, objective based tasks in a manner similar to, or superior to a human. Many of the methods being explored in the machine intelligence space require large sets of labeled data to first train, and then classify inputs. Hierarchical Temporal Memory (HTM) is a biologically inspired machine intelligence framework which aims to classify and interpret streaming unlabeled data, without supervision, and be able to detect anomalies in such data.

In software HTM models, increasing the number of “columns” or processing elements to the levels …

Mastering Mycological Mysteries With Explorations Of Harpellales Associated With Culicidae And Other Dipterans In Idaho, Michael Mccormick

Boise State University Theses and Dissertations

Presented is the first field survey and laboratory-based study focused on Harpellales gut fungi found in Culicidae (mosquito) larvae collected from various parts of Idaho. Overall, 34 sites were sampled ranging from urban storm drains and irrigation puddles to pristine stream-side puddles. These sites yielded 17 different species of mosquitoes and three previously described species of gut fungi. Three species of mosquitoes were the first recorded observations as hosts of the following gut fungi: Culiseta alaskaensis was infested with Zancudomyces culisetae (from Renwyck Creek), Culex tarsalis with Smittium culicis (from Cottonwood Creek), and Ochleratus sp. with Smittium minutisporum (from Bear …

Phosphate Removal And Recovery Using Immobilized Phosphatebinding Proteins, Kaushik Venkiteshwaran, Nilisha Pokhrel, Faten Hussein, Edwin Antony, Brooke K. Mayer

Biological Sciences Faculty Research and Publications

Progress towards a more circular phosphorus economy necessitates development of innovative water treatment systems which can reversibly remove inorganic phosphate (P_i) to ultra-low levels (<100 μg L⁻¹), and subsequently recover the P_i for reuse. In this study, a novel approach using the high-affinity E. coli phosphate binding protein (PBP) as a reusable P_i bio-adsorbent was investigated. PBP was expressed, extracted, purified and immobilized on NHS-activated Sepharose beads. The resultant PBP beads were saturated with P_i and exposed to varying pH (pH 4.7 to 12.5) and temperatures (25–45 °C) to induce P_i release. Increase in …

Chitosan/Ag-Hydroxyapatite Nanocomposite Beads As A Potential Adsorbent For The Efficient Removal Of Toxic Aquatic Pollutants, Long Li, Jibran Iqbal, Ying Zhu, Peng Zhang, Wanchao Chen, Amit Bhatnagar, Yiping Du

All Works

© 2018 Elsevier B.V. In the present study, the potential of synthesized chitosan/Ag-substituted hydroxyapatite nanocomposite beads to remove basic dye, heavy metal and microbes from aqueous solutions was investigated. Beads were prepared in different ratios via embedding of Ag-hydroxyapatite (Ag-HA) into chitosan (CS) solution. The beads were characterized by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) in order to get an insight of the functional groups and morphology. Batch adsorption studies were conducted with copper ions (Cu (II)) and rhodamine B (RhB) dye by changing several parameters such as Ag-HA to Cs ratio, contact time, solution pH …

Desert Plant Bacteria Reveal Host Influence And Beneficial Plant Growth Properties, Abdul Aziz Eida, Maren Ziegler, Feras F. Lafi, Craig T. Michell, Christian R. Voolstra, Heribert Hirt, Maged M. Saad

All Works

© 2018 Eida et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Deserts, such as those found in Saudi Arabia, are one of the most hostile places for plant growth. However, desert plants are able to impact their surrounding microbial community and select beneficial microbes that promote their growth under these extreme conditions. In this study, we examined the soil, rhizosphere and endosphere bacterial communities of four native desert plants Tribulus terrestris, Zygophyllum simplex, …

Epilepsy, Identity, And The Experience Of The Body, Stephanie Kılınç, Carol Campbell, Alison Guy, Anna Van Wersch

All Works

© 2018 Elsevier Inc. Living with a chronic condition can challenge a person's identity, yet there is a paucity of research exploring this experience for people with epilepsy and particularly for those diagnosed in adulthood. Consequently, through an interpretative phenomenological approach, the current study aimed to explore what the experience of adult-onset epilepsy meant for a person's identity. Thirty-nine people with adult-onset epilepsy from across the UK took part in up to two semi-structured interviews. A modified form of interpretative phenomenological analysis was conducted and identified three themes: 1) disarming the impact of seizures considered strategies used to control seizure …

Application Of Nuclear Techniques To Environmental Plastics Research, Cm Lanctot, M Al-Sid-Cheikh, Ai Catarino, T Cresswell, Et Al

School of Biological and Marine Sciences

Plastic pollution is ubiquitous in aquatic environments and its potential impacts to wildlife and humans present a growing global concern. Despite recent efforts in understanding environmental impacts associated with plastic pollution, considerable uncertainties still exist regarding the true risks of nano- and micro-sized plastics (<5 mm). The challenges faced in this field largely relate to the methodological and analytical limitations associated with studying plastic debris at low (environmentally relevant) concentrations. The present paper highlights how radiotracing techniques that are commonly applied to trace the fate and behaviour of chemicals and particles in various systems, can contribute towards addressing several important and outstanding questions in environmental plastic pollution research. Specifically, we discuss the use of radiolabeled microplastics and/or chemicals for 1) determining sorption/desorption kinetics of a range of contaminants to different types of plastics under varying conditions, 2) understanding the influence of microplastics on contaminant and nutrient bioaccumulation in aquatic organisms, and 3) assessing biokinetics, biodistribution, trophic transfer and potential biological impacts of microplastic at realistic concentrations. Radiotracer techniques are uniquely suited for this research because of their sensitivity, accuracy and capacity to measure relevant parameters over time. Obtaining precise and timely information on the fate of plastic particles and co-contaminants in wildlife has widespread applications towards effective monitoring programmes and environmental management strategies.

Interactions Between Fire Severity And Forest Biota In The Central Sierra Nevada: Formation And Impact Of Small-Scale Fire Refugia And The Effect Of Fire On Forest Structure Predictive Of Fisher (Pekania Pennanti) Den Habitat, Erika M. Blomdahl

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fire is a natural and essential component of forests in western North America. Fire maintains biodiversity through the creation of different habitat types, and regular fire rotations reduce the accumulation of woody fuels and thick understory plant densities that give rise to catastrophic fire. The practice of fire exclusion has altered western forests and increased the risk of widespread change under rising temperatures projected for the 21^st century. To manage for the reintroduction of fire it is critical that we understand the interactions between fire and forest biota in recently fire-suppressed forests.

In Chapter 2, I studied the formation …

Comparing The Predictive Power Of Executive Function Assessment Strategies On Preschool Mathematics Performance, Jacob A. Esplin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A child’s executive function (aspects: working memory, response inhibition, and set-shifting between sets of rules) capabilities have been found to strongly relate to their mathematics skills. However, while the relationship has been strongly supported by researchers, a consensus has not been reached regarding the specifics of the relationship between executive function and math skills, including which executive function aspect is most predictive of mathematical performance and the differences in said relationship that might be found when examining both numeracy, such as counting skills and basic operations, and geometry skills. The lack of consensus may be in part because researchers have …

Landscape Planning For Climate Change Resilience In The Southern Rockies, Jeffrey D. Haight

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The unique species, ecosystems and landscapes of the Western United States are experiencing unprecedented pressures from climate change, creating new challenges for conservation. As temperatures rise and patterns of precipitation shift, plant and wildlife species have been shifting their ranges to new areas in search of more suitable climates, building groupings of species that are historically unfamiliar. These climate -driven migrations place an additional burden on species that are already threatened from habitat loss and other human-related activities. The impacts of climate change are of particular concern in landscapes that have long been conserved and managed based on the ecological …

The Relationship Of Gender-Based Microaggressions And Internalized Sexism On Mental Health Outcomes: A Mother-Daughter Study, Nicole D. Feigt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Subtle occurrences of discrimination, insults, and slights against gender can impact woman of all ages, although little research has been done on the mental health impacts of these events on adolescents or middle-aged women. Additionally, a person’s own views on sex roles and sexism may impact how these events affect them. The following study examined the relationship between mothers and daughters on variables related to ambivalent sexism, gender-based microaggressions, and anxiety and depression. One hundred two mothers and their adolescent daughters completed various online surveys through the use of a Qualtrics panel. The sample was fairly representative, with respondents varying …

New Ca-Id-Tims Detrital Zircon Constraints On Middle Neoproterozoic Sedimentary Successions, Southwestern United States, Abigail R. Bullard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three related sedimentary successions located in Arizona, Utah, and California were deposited in basins on proto-North America during the early rifting of Rodinia (~780 Mya). Previous detrital zircon U-Pb maximum ages for the units are inexact, making it difficult to piece together what happened at this point in Earth history.

We report better maximum age constraints on these units obtained by subjecting detrital zircons to high-precision CA-ID-TIMS analysis, which provide more exact ²⁰⁶Pb/²³⁸U ages. These new data significantly improve the precision for the base of the ChUMP units, with an average age of 775.63 ± 0.27 Ma …