Digital Commons Network^™

Optimization Of Detergent-Mediated Reconstitution Of Influenza A M2 Protein Into Proteoliposomes, Catherine Hirshfeld Crouch, Margaret H. Bost , '17, Tae H. Kim , '14, Bryan M. Green , '16, Douglas Stuart Arbuckle , '17, Carl H. Grossman, Kathleen P. Howard

Physics & Astronomy Faculty Works

We report the optimization of detergent-mediated reconstitution of an integral membrane-bound protein, full-length influenza M2 protein, by direct insertion into detergent-saturated liposomes. Detergent-mediated reconstitution is an important method for preparing proteoliposomes for studying membrane proteins, and must be optimized for each combination of protein and membrane constituents used. The purpose of the reconstitution was to prepare samples for site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) studies. Our goals in optimizing the protocol were to minimize the amount of detergent used, reduce overall proteoliposome preparation time, and confirm the removal of all detergent. The liposomes were comprised of (1-palmitoyl-2-oleyl-sn-glycero-phosphocholine (POPC) and 1-palmitoyl-2-oleyl-sn-glycero-3-[phospho-rac-(1-glycerol)] …

University Of Dayton Magazine, Winter 2018-19, University Of Dayton

University of Dayton Alumni Publications

The University of Dayton Magazine is published quarterly by the Office of University Marketing and Communications for alumni, parents, students, faculty/staff, trustees and other friends of UD. Articles in this issue include "Funkadelic Dayton," "Dan Curran: Grateful for the Gift of Life," "Restoring the Christmas Spirit," and "Lessons Learned: Reflections on a Life Acquired in and out of the Classroom," along with regular features (President's Commentary, Conversation Pieces, Letters, Commentary, View Finder, Where are you Reading?, Sports, Class Notes, Perceptions, Parting Words, and Connections).

A Multicase Study Exploring Women’S Narratives Of Infertility: Implications For Counselors, Staci L. Born, Christian Carotta, Kristine Ramsay Seaner

Counseling and Human Development Faculty Publications

Infertility affects 6.7 million women in the United States (Chandra, Copen, & Stephen, 2013). Women’s experiences with infertility are not only influenced by biological health factors, but also by social, cultural, and personal variables. Given the prevalence and complexity of infertility, additional research is needed to further examine the nuances of women’s experiences. The purpose of this multicase study, as informed by four individual cases, was to explore how women construct their infertility narratives. Review of reflective journals found five common elements: (1) Emotional Rollercoaster, (2) Mind-Body (Dis)Connection, (3) Secret Identity, (4) Supportive vs. Constrained Communication Patterns, and (5) Fatalistic- …

Classification Of Clinical Narratives Using Convolutional Neural Network, Nikit Rajiv Lonari

Computer Science and Engineering Theses - Archive

Patient safety is a key aspect for good consumer care. When an individual is hospitalized or receives medication the family wants the patient safety to be above all factors. For instance, a drug can do both either cure the disease or perhaps, give rise to an adverse event. A drug administered for an indicated condition has substantial power to reduce or cure a disease and further to prevent it from happening again in the future but at the risk of side effects. At present, there are several methods in patient safety and in particular in the area of signal detection …

Towards End-To-End Semi-Supervised Deep Learning For Drug Discovery, Xiaoyu Zhang

Computer Science and Engineering Theses - Archive

Observing the recent progress in Deep Learning, the employment of AI is surging to accelerate drug discovery and cut R&D costs in the last few years. However, the success of deep learning is attributed to large-scale clean high-quality labeled data, which is generally unavailable in drug discovery practices. In this thesis, we address this issue by proposing an end-to-end deep learning framework in a semi supervised learning fashion. That is said, the proposed deep learning approach can utilize both labeled and unlabeled data. While labeled data is of very limited availability, the amount of available unlabeled data is generally huge. …

Generating An Adaptive Path Using Rrt Sampling And Potential Functions With Directional Nearest Neighbors, Sandeep Chahal

Computer Science and Engineering Theses - Archive

Planning algorithms have attained omnipresent successes in several fields including robotics, animation, manufacturing, drug design, computational biology and aerospace applications. Path Planning is an essential component for autonomous robots. The problem involves searching the configuration space and constructing a desired collision-free path that connects two states (the start and the goal) for a robot to gradually navigate from one state to another. In global path planners, the complete path is computed prior to the robot set off. Sampling based planning like Rapidly Expanding Random Trees (RRT) and Probabilistic Road Maps (PRM) used for single or multi-query planning has gained popularity …

Evaporative Hotspot Cooling Of Microprocessors Using Electrowetting On Dielectric (Ewod), Arvind Venkatesan

Mechanical and Aerospace Engineering Dissertations - Archive

The increased use of smart devices and great strides in fabrication technologies have resulted in densely packed electronics. Combined with 3D packaging, high heat uxes have become the norm. The need for advanced thermal management solutions has risen. Most devices based on their load pro le and architecture are susceptible to non-uniformities in heat uxes. These non-uniformities with very high heat uxes are known as hotspots. Mitigation of hotspots is very important for the normal functioning and long term reliability of microprocessors. Multiple attempts have been made at developing microscale thermal solutions. Most prominent among them are microchannels and heat …

Direct Numerical Simulation Of Homogeneous Isotropic Turbulence - A Methodology And Applications, Sarah Moussa Hussein

Mechanical and Aerospace Engineering Dissertations - Archive

Turbulence has been a topic of scientific research for years. Characterized by unorganized chaotic motion and irregular fluctuations, it persists as one of the most challenging topics in fluid mechanics despite volumes of documented research and crucial findings. This begs the question: What is turbulence and why is it so challenging? Turbulence research studies cover a wide spectrum of branches from fundamental flow propagation to different turbulence interactions. This research project investigates the simplest class of turbulent flow studies, homogeneous isotropic turbulence. In a quest to advance the fundamental understanding of turbulence physics, a direct numerical simulation tool is developed. …

Design And Development Of Flexible Mems-Based Ph Sensor For Agricultural And Biomedical Applications, Shardul Sonu Pawar

Mechanical and Aerospace Engineering Theses - Archive

The purpose of this study is to demonstrate a novel method of using a flexible MEMS-based pH sensor for agricultural and biomedical applications. The first part of the thesis involves demonstration of the capability of our pH sensor for monitoring pH level change in the food product. The pH sensor we have developed consists of Iridium Oxide (IrOx) and Silver Chloride (AgCl) sensing electrodes. The sensor is designed to monitor and sustain long-term continuous usage, which makes it suitable for a wide variety of agricultural based applications. The electrochemical potential generated between IrOx and AgCl electrode is monitored and further …

Rational Design And Synthesis Of Biodegradable Polyurethane Based Biomaterials, Cancan Xu

Bioengineering Dissertations - Archive

Biodegradable polyurethanes have been applied in biomedical field since 1960s. In recent years, biodegradable polyurethanes have been widely investigated for tissue repair and regeneration because of their good mechanical properties, elasticity, biodegradability, biocompatibility and processability. Compared with some commonly used biodegradable polymers, such as poly(glycolide) (PGA), poly(lactide) (PLA), poly(ε-caprolactone) (PCL) and polyhydroxyalkanoates (PHA), the favorable aspects of biodegradable polyurethane are its mechanical, physicochemical and biological properties can be tailored by varying the three blocks in polyurethane backbone, diisocyanate, diol and chain extender. In this thesis, we focus on the development of different functional biodegradable polyurethanes to study their structure-property relationships …

Stimuli-Responsive Nanoparticles For Treatment Of Lung Cancer, Roshni Iyer

Bioengineering Dissertations - Archive

Despite extensive research and progress in anti-cancer therapeutics, an effective treatment for non-small cell lung cancer (NSCLC) remains elusive, resulting in poor 5-year patient survival rate. Towards this end, we have explored a therapeutic regimen to improve the effects of local concurrent chemo-radiation-therapy (CRT), by utilizing multifunctional stimuli-responsive nanoparticles (NPs) as drug carriers. We have also utilized lung cancer targeting strategies for site-specific drug delivery, mainly antibodies against the Ephrin-transmembrane receptor kinase (EphA2), that is over-expressed mainly on lung cancer cells, to promote the targeted delivery of drugs to only cancer cells, and eventually reduce the toxicity to healthy tissues. …

A Staring Contest With The Self: Karl Ove Knausgaard, Linguistic Inquiry And Word Count, And Explanatory Style, Colton Tyler Royle

English Theses - Archive

Shame as an emotional response in the late 2010s has been discussed in both high and low sectors, from a high level of shame with social media use in high school classrooms, to the lack of shame by the president of the United States. My thesis addresses this question by analyzing Karl Ove Knausgaard’s two autobiographical works, My Struggle and his Seasonal Quartet. Readers, critics, and Knausgaard himself have described his work as a focus on the shameful moments of his life. Using positive psychology’s term “explanatory style,” coined by Martin E.P. Seligman, I do a structural reading of explicit …

Truth And Strength In Vulnerability: Using Topoanalysis To Reveal The Need For Expanding The Modernist Literary Canon, Misty Dawnmarie Falkenstein

English Theses - Archive

In the late nineteenth and early twentieth centuries, the public role of women shifted dramatically. Women asserted themselves in politics, education, and work in a way foreign to their Victorian predecessors. Although these “New Women” altered the gender landscape and set in motion a new path for women that would continue even into the twenty-first century, their writing still goes largely unnoticed in the current study of the Modernist literary canon. This project makes a case for expanding the current Modernist literary canon to include more of these women, especially women of disenfranchised racial, ethnic, cultural, and economic groups. Writers …

The Monadnock Volume 60, December 2018, Clark University Geographical Society

The Monadnock (Clark Geographical Society)

Volume 60 of The Monadnock. The Monadnock was the alumni magazine of the Clark University Geographical Society (1927-1985) (2014-present).

A Gis-Based Framework Creating Green Stormwater Infrastructure Inventory Relevant To Surface Transportation Planning, Xiaofan Xu, Dylan S. P. Schreiber, Qing Lu, Qiong Zhang

Civil and Environmental Engineering Faculty Publications

The stormwater runoff that carries pollutants from the land adjacent to road transportation systems may impair the water environment and threaten the ecosystem and human health. A proper management approach like green stormwater infrastructure (GSI) can help control flooding and the runoff pollutants. One barrier for GSI analysis relevant to system-level surface transportation planning is the lack of the inventory of GSI in many U.S. cities. This study aims to develop a GIS-based framework for creating GSI inventory in a time and labor efficient way, different from the traditional survey-based method. The new proposed framework consists of three steps, including …

Epidemiological Evidence And Health Risks Associated With Agricultural Reuse Of Partially Treated And Untreated Wastewater: A Review, Anthony A. Adegoke, Isaac D. Amoah, Thor A. Stenström, Matthew E. Verbyla, James R. Mihelcic

Civil and Environmental Engineering Faculty Publications

The use of partially treated and untreated wastewater for irrigation is beneficial in agriculture but may be associated with human health risks. Reports from different locations globally have linked microbial outbreaks with agricultural reuse of wastewater. This article reviews the epidemiological evidence and health risks associated with this practice, aiming toward evidence-based conclusions. Exposure pathways that were addressed in this review included those relevant to agricultural workers and their families, consumers of crops, and residents close to areas irrigated with wastewater (partially treated or untreated). A meta-analysis gave an overall odds ratio of 1.65 (95% CI: 1.31, 2.06) for diarrheal …

Characterizing Young Adult Exposure And Engagement With Social Media Tobacco And Nicotine Product Marketing And Messaging, Stephanie L. Clendennen

Dissertations and Theses (Open Access)

Tobacco use remains a critical public health issue in the United States. Young adults are disproportionately affected by high rates of tobacco use and heavily targeted by tobacco marketing. Social media has become an important source of exposure to tobacco and nicotine product marketing and messaging for young adults. This dissertation examined the prevalence and socio-environmental characteristics associated with young adults’ exposure to and engagement with tobacco-related social media (paper 1); the prospective associations between young adults’ exposure and engagement and tobacco and nicotine product use (paper 2); and young adults’ experiences with tobacco and nicotine product messaging on social …

White-Nose Syndrome Detected In Bats Over An Extensive Area Of Russia, Veronika Kovacova, Jan Zukal, Hana Bandouchova, Alexander D. Botvinkin, Markéta Harazim, Natália Martínková, Oleg L. Orlov, Vladimir Piacek, Alexandra P. Shumkina, Mikhail P. Tiunov, Jiri Pikula

KIP Articles

No abstract provided.

Comparison Mining From Text, Maksim Tkachenko

Dissertations and Theses Collection (Open Access)

Online product reviews are important factors of consumers' purchase decisions. They invade more and more spheres of our life, we have reviews on books, electronics, groceries, entertainments, restaurants, travel experiences, etc. More than 90 percent of consumers read online reviews before they purchase products as reported by various consumers surveys. This observation suggests that product review information enhances consumer experience and helps them to make better-informed purchase decisions. There is an enormous amount of online reviews posted on e-commerce platforms, such as Amazon, Apple, Yelp, TripAdvisor. They vary in information and may be written with different experiences and preferences.

If …

The Impact Of Whoop Technology On Sleep, Recovery, And Performance In Naia Baseball Players, Nolan Harms

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

Many key factors – including but not limited to – sleep, nutrition, travel, stress, and practice influence the optimization of athletic performance. Although previous studies have investigated the use of wearable technology in sport to track several such factors, peer-reviewed research specific to WHOOP technology is limited at best. The purpose of this study was to examine the impact of WHOOP technology on sleep, recovery, and batting performance in healthy NAIA baseball players. Data was collected over the course of 4 weeks of in-season play on 10 varsity NAIA baseball players (20.40 ± 0.97 years). All games – 18 total …

Contextual Parentage, Jeffrey A. Parness

College of Law Faculty Publications

A parentage determination can be used in a dispute over child custody/visitation/parental responsibility allocation opportunities, over child support duties, over heirship in probate, or over standing to pursue tort remedies.

Intestacy, Wills, And Intent: A Short Comment On Wright & Sterner, David Horton

ACTEC Law Journal

No abstract provided.

Identifying And Assessing Conflicts Between Future Development And Current Migratory Bird Habitat Around Farmington Bay, Utah, Aubin A. Douglas

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Every year, the Great Salt Lake (GSL) and its associated wetlands provide critical habitat for over 250 migratory bird species from both the Pacific and Central Flyways. The GSL borders the Wasatch Front, which is the fastest growing and most populous region in Utah. To support the ever-increasing working population, the government of Utah aspires to increase the robust economic growth of the region through economic incentives and development of infrastructure. As this area continues to develop, greater pressure will be placed on the surrounding natural resources, including the GSL, its wetlands, and the open space and agricultural land that …

Methanogens, Plausible Extraterrestrial Life Forms On Mars, And Their Tolerance To Increasing Concentrations Of Illite Clay, Chandler Kern

Biological Sciences Undergraduate Honors Theses

Methanogens, some of Earth’s most primitive prokaryotic organisms, are candidates for possible life forms capable of inhabiting Mars. Specifically, four different species (Methanobacterium formicicum, Methanococcus maripaludis, Methanosarcina barkeri, Methanothermobacter wolfeii) were analyzed for their tolerance to the presence of illite clay. Illite is a crystalline mineral that has been identified from regions of Mars’s surface. Results indicated that all four species grew with some success in the illite at different concentrations. This experimentation with methanogens’ abilities to survive and reproduce in the presence of illite allows for a more accurate understanding of the potential capability of microbial …

Qed: The Research Annual Of The Uta Honors College 2018, Uta Honors College

QED: The Research Annual of the UTA Honors College - Archive

No abstract provided.

Synergistic Negative Effects Of Thermal Stress And Altered Food Resources On Echinoid Larvae, Colette Feehan, Zoe Ludwig, Suzannah Yu, Diane K. Adams

Department of Biology Faculty Scholarship and Creative Works

Multiple changes to the marine environment under climate change can have additive or interactive (antagonistic or synergistic) effects on marine organisms. Prompted by observations of anomalously warm sea temperatures and low chlorophyll concentrations during the 2013–2016 warm “Blob” event in the Northeast Pacific Ocean, we examined the combined effects of thermal stress and a shift in food resources on the development of a larval echinoid (Strongylocentrotus droebachiensis) in the laboratory. A high concentration of phytoplankton yielded faster echinus rudiment development at warm versus historical temperature, indicating a mitigating effect of abundant food on thermal stress; however, low phytoplankton concentration or …

Smartphone And Social Media Use And Its Health Associations, Cory Newell

Psychology Theses - Archive

While mobile phones offer users communication, information, and entertainment almost instantaneously, there is a growing concern about the impact smartphone and social media use has on mental and physical health. This study addressed gaps in the literature by simultaneously measuring objective and subjective smartphone use rather than relying on subjective reports alone, and then assessed how they were differentially related to health. Generally, it was predicted that measures of smartphone use would be reliable and valid, and that higher use would predict worse health. Overall, a diverse sample (39% White, 22.1% Asian, and 16.9% African American) of 136 young adults …

A Qualitative Systematic Review And Narrative Synthesis Of The Effectiveness Of Interventions For Non-Intimate Parter, Scott Sainato

Social Work Dissertations - Archive

Social Workers have an ethical obligation to “monitor and evaluate policies, the implementation of programs, and practice interventions” (National Association of Social Workers, 2017, 5.02A). This study seeks to meet ethical standards for social workers as well as advance the general knowledge base in the area of Non-Intimate Partner – Youth family Violence (NIP-YFV) by conducting the first systematic review and narrative synthesis in the area of NIP-YFV. The following research will identify and explain why certain interventions are most effective in addressing NIP-YFV. Discussion and conclusion will detail the implications this study has on social work practitioners, policy makers, …

Polarization And Nuclear Fear: How Partisan Message Processing Affects Public Trust In Government, Ian Trevor Owens

Political Science Theses - Archive

Scholars have found that an environment that appears increasingly polarized in the United States has been accompanied by a decline in institutional trust over several decades. Scholarship involving partisanship and trust suggests that out-party groups are less receptive to political messages than in-groups. In the wake of the false emergency alert in Hawaii that sent citizens into a panic for 38 minutes, messaging and nuclear issues remain a salient topic for study. This thesis presents the results of an experiment (N = 2,310) investigating how political trust is influenced by the partisan interpretation of a message. Survey respondents were presented …

Online Bioanalytical Mass Spectrometry Methods For Analysis Of Small And Large Biomolecules Using One- And Two-Dimensional Liquid Chromatography, Yehia Zakaria Baghdady

Chemistry & Biochemistry Dissertations - Archive

In recent years, the innovation in biomarkers and biotherapeutics has increased the demand for state-of-the-art analytical platforms to achieve reliable and accurate discoveries and quantitative analysis methods. The challenges in their determinations are not limited to the large number of small and large biomolecules but also involve the great heterogeneity in chemical properties and abundance of those analytes. Liquid chromatography and mass spectrometry have advanced significantly to be the most common platform for analyzing biological samples. However, there are still many unmet challenges that that require application of novel bioanalytical methods to fill many gaps in online sample preparation, chromatographic …