Digital Commons Network^™

Effects Of Global O-Glcnacylation On Galectin Gene-Expression Profiles In Human Cancer Cell Lines, Ali A. Sherazi, Komal A. Jariwala, Amanda N. Cybulski, Justin W. Lewis, Jim Karagiannis, Robert C. Cumming, Alexander V. Timoshenko

Paediatrics Publications

Background/Aim: The effects of O-linked β-N-acetyl-D-glucosamine (O-GlcNAc) transferase (OGT) and O-GlcNAcase (OGA) inhibitors on galectin gene expression profiles were examined in MCF7, HT-29, and HL-60 cancer cell lines. Materials and Methods: Cell cultures were treated for 24 h with OGA inhibitor thiamet G or OGT inhibitor 2-acetamido-1,3,4,6-tetra-O-acetyl-2-deoxy-5-thio-α-D-glucopyranose, and global O-GlcNAc levels and expression of galectin genes were determined using an immunodot blot assay and real-time quantitative polymerase chain reaction. Results: Two galectin genes, LGALS3 in MCF7 cells and LGALS12 in HL-60 cells, were up-regulated by O-GlcNAc, whereas other cell-specific galectins were unresponsive to changes in O-GlcNAc level. Of interest, basal …

Brca1 And Brca2 5′ Noncoding Region Variants Identified In Breast Cancer Patients Alter Promoter Activity And Protein Binding, Leslie J. Burke, Jan Sevcik, Gaetana Gambino, Emma Tudini, Eliseos J. Mucaki, Ben C. Shirley, Phillip Whiley, Michael T. Parsons, Kim De Leeneer, Sara Gutiérrez-Enríquez, Marta Santamariña, Sandrine M. Caputo, Elizabeth Santana Dos Santos, Jana Soukupova, Marketa Janatova, Petra Zemankova, Klara Lhotova, Lenka Stolarova, Mariana Borecka, Alejandro Moles-Fernández, Siranoush Manoukian, Bernardo Bonanni, Stacey L. Edwards, Marinus J. Blok, Thomas Van Overeem Hansen, Maria Rossing, Orland Diez, Ana Vega, Kathleen B.M. Claes, David E. Goldgar, Etienne Rouleau

Biochemistry Publications

© 2018 The Authors. Human Mutation published by Wiley Periodicals, Inc. The widespread use of next generation sequencing for clinical testing is detecting an escalating number of variants in noncoding regions of the genome. The clinical significance of the majority of these variants is currently unknown, which presents a significant clinical challenge. We have screened over 6,000 early-onset and/or familial breast cancer (BC) cases collected by the ENIGMA consortium for sequence variants in the 5′ noncoding regions of BC susceptibility genes BRCA1 and BRCA2, and identified 141 rare variants with global minor allele frequency < 0.01, 76 of which have not been reported previously. Bioinformatic analysis identified a set of 21 variants most likely to impact transcriptional regulation, and luciferase reporter assays detected altered promoter activity for four of these variants. Electrophoretic mobility shift assays demonstrated that three of these altered the binding of proteins to the respective BRCA1 or BRCA2 promoter regions, including NFYA binding to BRCA1:c.-287C>T and PAX5 binding to BRCA2:c.-296C …

Father–Daughter Bonds: A Comparison Of Adolescent Daughters’ Relationships With Resident Biological Fathers And Stepfathers, Cynthia G. Campbell, Elizabeth J. Winn

Psychological Sciences Faculty Publications and Presentations

Objective: To investigate whether the interpersonal dynamics of closeness are different in stepfather–stepdaughter versus father–daughter relationships during adolescence.

Background: Establishing a general process model of the relational factors contributing to greater closeness between fathers and daughters is a preliminary step toward examining variations in such processes.

Method: The data were from the National Longitudinal Study of Adolescent to Adult Health (ADD Health), a nationally representative sample of adolescents. Respondents were female adolescents who were living with either a biological father (n = 1,881) or stepfather (n = 273) and reported on the availability and involvement of their (step)fathers, …

Sequential Regulation Of Maternal Mrnas Through A Conserved Cis-Acting Element In Their 3′ Utrs, Gabriele Fuchs, Pooja Flora, Siu Wah Wong-Deyrup, Elliot Todd Martin, Ryan J. Palumbo, Mohamad Nasrallah, Andrew Oligney, Patrick Blatt, Dhruv Patel, Prashanth Rangan

Biological Sciences Faculty Scholarship

Maternal mRNAs synthesized during oogenesis initiate the development of future generations. Some maternal mRNAs are either somatic or germline determinants and must be translationally repressed until embryogenesis. However, the translational repressors themselves are temporally regulated. We used polar granule component (pgc), a Drosophila maternal mRNA, to ask how maternal transcripts are repressed while the regulatory landscape is shifting. pgc, a germline determinant, is translationally regulated throughout oogenesis. We find that different conserved RNA-binding proteins bind a 10-nt sequence in the 3′ UTR of pgc mRNA to continuously repress translation at different stages of oogenesis. Pumilio binds …

Habitat Suitability Analysis For Mountain Lions (Puma Concolor) Recolonization/ Reintroduction In Minnesota, Mbongowo J. Mbuh, Neil Vruno

Geography & Geographic Information Science Faculty Publications

The mountain lion range once extended throughout the state of Minnesota. The breeding population has been greatly reduced with time, new roads, and timber harvesting, which have broken large tracts of contiguous forest into isolated patches that are too small and no longer suitable for the breeding mountain lion population. The objective of this study is to use suitability analysis to determine the most suitable habitat to conserve mountain lion populations threatened by habitat fragmentation. To attain our objective, we created three sub models that contribute to the overarching goal of the suitability model. A habitat sub model was developed …

Effect Of Postural Control Position And Blood- Sampling Arm Position On Change In Plasma Volume From Supine Rest Through A Cycling Bout, William Sullivan, Michele Fisher

Department of Exercise Science and Physical Education Scholarship and Creative Works

The objective of this study was to determine any interaction between a postural control period and bloodsampling arm position on change in plasma volume (ΔPV) from supine rest through a bout of cycling. The subjects underwent two different testing protocols. During the control test, each subject lay supine and then sat upright, each for 30 min before cycling for 20 min at ~70% of age-predicted maximal heart rate (HR max). During the non-control test, each subject cycled immediately after 30 min of supine rest. During seated rest and exercise, one arm was supported at heart level (Horizontal), the other was …

University At Albany Stars Report, University At Albany, State University Of New York

STARS reports

Gold Rating: STARS Version 2.1

The Sustainability Tracking, Assessment & Rating System (STARS®) is a transparent, self-reporting framework for colleges and universities to gauge relative progress toward sustainability. STARS was developed by AASHE with broad participation from the higher education community.

STARS is designed to:

• Provide a framework for understanding sustainability in all sectors of higher education.

• Enable meaningful comparisons over time and across institutions using a common set of measurements developed with broad participation from the campus sustainability community.

• Create incentives for continual improvement toward sustainability.

• Facilitate information sharing about higher education sustainability practices and …

Predicting Kidding Date Using Prepartum Milk Calcium Concentrations And Comparing Kid Growth To Colostrum Quality In Goats, Justin Hamm

Animal Science Undergraduate Honors Theses

Abstract

Goats have a very long history with mankind and are commonly used as a nutritious red meat source around the world. The supply of goat meat in the United States does not meet the growing demand from ethnic consumers. This provides an economic opportunity in Arkansas agriculture because goats are uniquely adapted to the Ozark environment. There is a wide gap in caprine research which facilitated the conception of the study’s objectives to determine whether prepartum milk in goats showed a sudden rise in calcium levels within 24 to 48 hours of kidding and to ascertain whether an increased …

Cole Train | V.15, No.2 (Fall 2018), Houston Cole Library

Library Annual Reports & Newsletters

No abstract provided.

Designing A Protected Area To Safeguard Imperiled Species From Urbanization, Stephanie S. Romanach, Brad Stith, Fred A. Johnson

United States Geological Survey: Staff Publications

Reserve design is a process that can address ecological, social, and political factors to identify parcels of land needed to sustain wildlife populations and other natural resources. Acquisition of parcels for a large terrestrial reserve is difficult because it typically occurs over a long timeframe and thus invokes consideration of future conditions such as climate and urbanization changes. In central Florida, the U.S. government has authorized a new protected area, the Everglades Headwaters National Wildlife Refuge. The new refuge will host important threatened and endangered species and habitats, and will be located to allow for species adaptation from climate change …

Defining The Genetic Regulation Of Appressorium Formation In Cercospora Zeae-Maydis, Hazel Apiyo Buyu

Graduate Theses and Dissertations

Cercospora zeae-maydis is one of the primary pathogens associated with gray leaf spot, one of the most damaging foliar diseases of maize in the world. Gray leaf spot can be managed to some extent by cultural practices and fungicide applications. To infect maize, C. zeae-maydis grows towards stomata and forms infectious structures, termed appressoria, over stomatal pores. Prior research on the pathogen revealed that appressorium formation is crucial for foliar infection. Although several genes involved in pathogenesis have been identified in C. zeae-maydis, the molecular regulation of appressorium formation in this pathogen is poorly understood. Specifically, how the fungus senses …

Molecular Basis Of Pathogenesis And Host Determination In Cercospora Sojina: From Phenotypic To Genotypic Patterns, Wagner Calegari Fagundes

Graduate Theses and Dissertations

Frogeye leaf spot (FLS), caused by Cercospora sojina, is an important and recurrent disease of soybean in many production regions. Genetic resistance is potentially one of the most cost-effective and sustainable strategies to control FLS. However, C. sojina has already demonstrated the ability to overcome resistance conveyed by single R-genes (resistance genes) of soybeans, followed by the emergence of new physiological races. Although understanding population genomics and the virulence gene inventories in fungal plant pathogens is extremely important to improve disease control measures, studies regarding host specificity and pathogenesis in C. sojina are very limited. Therefore, the overarching goal of …

Understanding And Predicting Nematode Damage On Soybean Using Spatially Weighted Analysis, Barry Boney

Graduate Theses and Dissertations

Aerial imagery offers great potential as a predictive scouting method and could allow growers to better understand crop performance over time. Evidence suggests that the seed treatments fluopyram and abamectin result in decreased reproduction and root galling by Meloidogyne incognita, but yield protection in fields with higher or different nematode pressure is unclear. The objective of this work was to determine the efficacy of these seed treatments compared to 1,3-dichloropropene (1,3-D) applied site-specifically and then predict where these might best be applied to other fields. In a soybean field infested with M. incognita, apparent electrical conductivity was highly correlated with …

Interfacial Tailoring Of Lithium-Ion Batteries By Atomic/Molecular Layer Deposition, Qian Sun

Graduate Theses and Dissertations

Lithium-ion batteries (LIBs) are promising energy storage devices, which play significant roles in addressing problems related to fossil fuels depletion and environmental pollution. Since the 1990s, LIBs have attracted great attention for many applications. Nowadays, LIBs are dominating portable electronics, having several advantages over their forerunners, such as high voltage (3.3~4.2 V) [1,2], low self-discharge (< 5~10 %/month) [3,4], wide operation temperature (-20~60 °C) [5,6], and fast charge/discharge rate [7,8]. However, LIBs deliver an energy density of 100-220 Wh/kg in practice to date, which is far from their theoretical ones, thus hindering their further applications in electric vehicles. Additionally, LIBs have been plagued by other problems, such as intolerance to overcharge/overdischarge, low heat resistance, lithium dendrites growth, large volume change of the silicon anode, large polarization and even safety problems.

Atomic layer deposition (ALD) and molecular layer deposition (MLD) are two important techniques, both proceeding in self-limiting gas-solid reactions and exhibiting excellent capabilities for ultra-thin films, conformal coatings, and controllable growth. They can be employed to address the problems of LIBs mentioned above by …

A Generative Statistical Approach For Data Classification In A Biologically Inspired Design Tool, Marvin Manuel Arroyo Rujano

Graduate Theses and Dissertations

The objective of the research this thesis describes is to find a way to classify text-based descriptions of biological adaption to support Biologically Inspired design. Biologically inspired design is a fairly new field with ongoing research. There are different tools to assist designers and biologists in bio-inspired design. Some of the most common are BioTRIZ and AskNature. In recent years, more tools have been proposed to aid and make research in the field easier, for example, the Biologically Inspired Adaptive System Design (BIASD) tool. This tool was designed with the goal of helping designers in early design stages generate more …

Epidemiological Studies Of Soybean Vein Necrosis Virus And Potential Resistance Mechanisms To Its Vector Neohydatothrips Variabilis (Beach), Jing Zhou

Graduate Theses and Dissertations

Soybean (Glycine max (L.) Merrill) is one the most important crops in global agriculture with annual production of over 260 million metric tons. As the dependence of a growing global population to soybean has increased, so does the importance of soybean diseases and pests. Over 200 pathogens attack soybean; among them, viruses pose a major threat to the soybean industries accounting for approximately 10% of the annual yield reduction caused by diseases in the past two decades. Soybean vein necrosis virus (SVNV) is a relatively newly discovered virus causing the homonymous disease. The widespread occurrence of the disease in major …

Towards Environmentally Sustainable And Cost-Effective Food Distribution In The U.S., Jasmina Burek

Graduate Theses and Dissertations

Distribution centers (DCs) and supermarkets have an important role in food sustainability, but no previous research has accounted for their environmental impact. The purpose of this research was to assess environmental sustainability of grocery, perishables, and general merchandise DCs; to estimate food storing and retailing impact; and to provide cost-effective strategies to reduce DCs’ environmental impacts. The importance and relevance of the research is threefold: improving sustainability of DCs, food storing, and food retailing. The main method used in this research was the life cycle assessment (LCA) method. An initial study calculated environmental impacts of the Wal-Mart Stores, Inc. DCs, …

Assessing Biofiltration Without Ozonation For Removal Of Trihalomethane Precursors In Drinking Water At The Beaver Water District Drinking Water Treatment Plant, Sana Ajaz

Graduate Theses and Dissertations

Biofiltration without pre-ozonation has the capability to remove natural organic matter (NOM) fractions that serve as precursors of disinfection byproducts (DBPs), which include the four regulated trihalomethanes (THMs) and dichloroacetonitrile (DCAN). Rapid small-scale column tests (RSSCTs) and Pilot Plant filters operated at empty-bed contact times (EBCTs) of 4, 8, and 16 minutes were used to evaluate the performance of nutrient-amended (free ammonia and phosphorus) biofiltration for THM and DCAN precursor removal, as measured using formation potential (FP) tests. NOM surrogates – which include dissolved organic carbon (DOC), specific ultraviolet absorbance (SUVA254) and fluorescence-PARAFAC components – were measured weekly throughout the …

Graduate Internship Report- Maxwell High School, Amber Charter

Agricultural Education: Graduate Internship Reports

This internship and project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Maxwell High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Maxwell High School, the agriculture program and the community.

Treating Incarcerated Women With Co-Occurring Disorders, Jennifer Nichols

Counselor Education Capstones

Through a literature review, the treatment needs of women who are incarcerated with a dual diagnosis, both mental health and substance use disorder, will be addressed and evaluated. Specifically, this literature review will examine the impact of trauma and mental health disorders on creating addictive patterns. Since it is common for incarcerated women to have dual diagnoses, there is a need for effective and reachable treatment modalities when approaching incarcerated women. Much of the jail/prison sentences do not specifically address the recovery and treatment demands the women need to overcome trauma and substance use. This lack of availability typically renders …

Clemson Commencement Program,December 2018, Clemson University

Clemson Commencement Programs

No abstract provided.

Improvements In The Root Morphology, Physiology, And Anatomy Of Platycladus Orientalis Seedlings From Air-Root Pruning, Zhipei Feng, Xitian Yang, Hongyan Liang, Yuhua Kong, Dafeng Hui, Jiabao Zhao, Erhui Guo, Beibei Fan

Biology Faculty Research

Air-root pruning (AP) has been identified as an effective technique for enhancing root growth and development. However, little information is available regarding the temporal changes in the root system of Platycladus orientalis (L.) Franco under AP. We performed integrated morphological, physiological, and anatomical analyses of the roots in P. orientalis seedlings that had been air-root pruned for 120, 150, and 190 days. Our results found that the whole root length, number of root tips, and root surface area of AP seedlings at 120, 150, and 190 days were higher than those of the non–root-pruned (NP) seedlings (P < 0.05), but the average root diameter did not differ significantly between the treatments. Compared with NP treatment, AP increased the root length, surface area, number of tips, and specific root length of the ≤0.5 mm diameter roots in P. orientalis during the experimental periods (P < 0.05), but those of 0.5- to 1-mm-diameter roots were only increased on day 190 (P < 0.05). The AP plants also exhibited higher root vitality and proportion of live fine roots than the NP plants (P < 0.05). Our anatomical evaluation of the ≤0.5 mm roots and taproots revealed features that could account for the morphological differences found between the AP and NP plants. In conclusion, our results indicate that air-root pruning induced changes in the roots that promote the root system development in P. orientalis compared with the NP treatment during the experimental period. These results thus provide experimental evidence to support the use of AP in P. orientalis seedlings.

Biological Biomedical Seminar | Wintering Birds As Sentinels Of Climate Change, Dec. 3, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Science Café | Detecting Deception, Dec. 5, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Newsletter, Fall/Winter 2018, Jordan Orris

Newsletters of the Graduate School

No abstract provided.

Reimagining Econ. 101 – Indigenous, Buddhist, Jesuit, Secular Education, Susan Jacobson

Jesuit Higher Education: A Journal

A basic econ course, because it is a foundation for informed citizenship, is a requirement in many liberal arts colleges. Historically, orthodox economic theory has heavily influenced this course. But this narrow and theoretical approach to economics is not particularly useful in addressing the glaring challenges of inequality and global warming or inspiring our students to engage in solutions to social problems. This paper presents some ideas for professors who, after exposing students to the content of mainstream economics, want to challenge students to question the foundational assumptions and the consumer culture they live in by exposing them to an …

2018 Fall Newsletter, Morehead State University. Nursing Department.

Nursing Department Publications Archive

2018 Fall Newsletter of the Department of Nursing at Morehead State University.

Applications Of Passive Solar Energy And Reclaimed Heat From Livestock Manure Decomposition, Naomi Elizabeth Schalle

Honors Theses

One energy intensive part of raising livestock in colder climates is keeping water warm and easily accessible to the animals in the winter. The objective of this project is to increase the sustainability of livestock farms by developing and implementing an energy conservation measure to decrease electric energy usage on the heating of water for livestock in the winter. One thing farmers have in excess is manure, an abundant and renewable resource for the farmers. This project will capitalize on that excess to encourage a creative use of resources often seen as waste, through implementing aspects of sustainability and conservation …

The Father Of Pan‐Africanism: Rev. Edward Wilmot Blyden, Rafi Rahman

Journal of the Black Catholic Theological Symposium

Rev. Edward Wilmot Blyden was born in the Virgin Islands, was ordained a Presbyterian minister, and became a leader in the Pan- Africanism movement. This paper argues that Rev. Blyden is an important figure within contemporary Black Church Studies because of his sui generis posture towards Islam, Pan-Africanism, and Black Nationalism. The felicitous Pan-African accolades attributed to Rev. Blyden by the Black Church are a result of his novel Afro-centric and inclusive interfaith hermeneutical posture and writings from the period of Reconstruction through the Progressive Era. Rev. Blyden’s acceptance of non-Christian faith traditions within a Pan-African context supported and, more …

Entrepreneurship And The Effects Of Nature And Nurture, Adam Guillory

Undergraduate Honors Theses

This paper provides a systematic review of studies and opinions on the debate over whether entrepreneurs are born or made. Attention has been paid to reducing bias and locating as many credible sources as possible. Unfortunately, peer reviewed, quantitative studies and data on this topic seem to be lacking. Research on whether or not secondary, formal education is required in entrepreneurship and if so, the effects are also analyzed. After much review, information on both sides of the argument seems to be well founded and evenly split. An audience polled before and after an argument on the subject was also …