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Regulation Of Microvesicle Particle Release In Keratinocytes, Azeezat Afolake Awoyemi

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Microvesicle particles (MVPs) are produced from cellular membranes and are thought to mediate cell-cell communication, including in response to stressors such as UVB radiation and thermal burn injury. Previous studies have shown that stress-induced MVP release requires the platelet-activating factor (PAF) receptor in human keratinocytes and that pharmacological inhibition of acid sphingomyelinase (ASM) blocked this release. To validate a genetic role for ASM in MVP release, we used CRISPR-Cas9 gene silencing in human keratinocytes and primary fibroblasts derived from ASM-knockout mice. Though MVP release was partially blocked in ASM-deficient mouse fibroblasts, the inability to fully knockdown ASM in HaCaT cells …

M1 To M2 Macrophage Induction Using Retinoic Acid And Mesenchymal Stem Cells Loaded On An Electrospun Pullulan/Gelatin Scaffold To Promote Healing Of Chronic Wounds, Kaivon Assani

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Modulation of macrophage polarization is required for effective tissue repair and regenerative therapies. Conversion of macrophages from inflammatory M1 to fibrotic M2 phenotype could help in diseases such as chronic wound which are stuck in inflammatory state. During the inflammatory phase, macrophages are of the inflammatory phenotype (M1) and distribute pro-inflammatory cytokines including TNF-a and IL1[beta] which are microbicidal and recruit/activate cells. In normal wound healing macrophages then switch to a fibrotic phenotype (M2) promoting wound closure by angiogenesis, and matrix deposition. Chronic wounds are a major biological and financial burden to both patients and the health care system, costing …

Effect Of Dimensionality On In Vitro Growth Environment And Mesenchymal Stem Cell Function, Fatema Tuj Zohora

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The use of the standard two dimensional (2D) cell culture has laid down the fundamentals of molecular and cell biology. However, recent advances in cell-based regenerative medicine raises the concern on deconstructing cellular behaviors in more physiologically relevant three dimensional (3D) microenvironments. Differences in cell response in 2D versus 3D systems arise from the perturbations in gene expression patterns that stem from how cells sense their underlying 2D or surrounding 3D matrices and adjust their phenotypes accordingly. Thus, cells are no longer considered as a solitary entity of genome but a context arises from a combinatorial interactions of cell-ECM, cell-cell, …

Multitrophic Impacts Of An Emerging Invasive Plant, Elizabeth J. Roberson

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The invasion of novel habitats by non-native plant species is a worldwide problem with serious economical and ecological implications. Broad biotic and abiotic filters contribute to the overall invasibility of non-native species. Invasive plants have the ability to rapidly establish themselves and outcompete their native counterparts as result of their relationship with herbivorous arthropods. Because invasive plants displace native plants, reduce overall plant diversity, and alter vegetation structure, this can have cascading effects on insect herbivores, particularly those that rely on one or a few food plants, and arthropod predators. The genus Euonymus (Celastraceae) is comprised of 130 species. Euonymus …

Transcriptional Characterization Of Osteogenic And Adipogenic Differentiation Of Human Bone Marrow Derived Mesenchymal Stem Cells In 2d And 3d Peptide Hydrogel Culture System, R. M. Imtiaz Karim Rony

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Mesenchymal stem cells (MSCs) are adult multipotent stem cell that can differentiate into mesodermal lineages such osteoblast, adipocytes, and chondrocytes, or can be transdifferentiated into clinically relevant lineages such as cardiac or neural cells using in vitro reprogramming techniques. In addition to the multilineage differentiation potential, MSCs from most tissue origins such bone marrow derived mesenchymal stem cells (BM-MSCs) or adipose derived mesenchymal stem cells (AD-MSCs) have immune modulatory functions which indicate their promise in clinical applications for cell-based therapy, tissue engineering, and regenerative medicine. In recent years, three-dimensional (3D) cell culture systems, which utilize 3D scaffolds or hydrogels to …

Functionalization And Characterization Of Chemical Vapor Deposited Graphene Sheets Towards Application In Chemical Vapor Sensing, Nicholas Alexander Engel

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Chemical/biological sensors serve many purposes in protecting machinery, the environment, and human life/wellness. Graphene, a two-dimensional (2-D) material made up of carbon atoms in a honeycomb-like lattice, is promising for applications to chemical/biological sensing due to its unique properties. Functionalization of graphene by surface decorating with nanoparticles and increasing interior adsorption sites can tailor its catalytic activity and electrical properties, and hence, important for detecting and distinguishing trace hazardous gases. This research introduces two different approaches to functionalize graphene towards enhanced sensitivity and selectivity of graphene-based sensors. The morphologies, structures, and electrical properties of the functionalized graphene are systematically characterized. …

The Feasibility Of Dementia Caregiver Task Performance Measurement Using Smart Gaming Technology, Garrett G. Goodman

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Dementia caregiver burnout is detrimental to both the familial caregiver and their loved ones with dementia. As the population of older adults increases, both the number of individuals with dementia and their corresponding caregivers increase as well. Thus, we are interested in developing a potential tool to non-invasively detect signs of caregiver burnout using a mobile application combined with machine learning. Hence, the mobile application "Caregiver Assessment using Smart Technology" (CAST) was developed which personalizes a word scramble game. The CAST application utilizes a heuristically constructed Fuzzy Inference System (FIS) optimized via a Genetic Algorithm (GA) to provide an individualized …

Fast Voltage-Gated Sodium Channel Currents And Action Potential Firing In R6/2 Skeletal Muscle, Eric Joshua Reed

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Huntington’s disease (HD) is a degenerative disorder caused by expanded CAG repeats in the Huntingtin gene, which results in cognitive problems as well as muscle weakness, chorea, rigidity, and dystonia. Most research in HD has focused on neurodegeneration, but recent studies have found peripheral defects that may help explain the debilitating motor symptoms of HD. We have shown that skeletal muscle from the R6/2 transgenic mouse model of HD is hyperexcitable due to decreases in resting chloride and potassium currents. Other groups have speculated that the fast voltage-gated sodium channels may be affected in Huntington’s disease as well. To fully …

Development Of A Human-Ai Teaming Based Mobile Language Learning Solution For Dual Language Learners In Early And Special Educations, Saurabh Shukla

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Learning English as a secondary language is often an overwhelming challenge for dual language learners (DLLs), whose first language (L1) is not English, especially for children in early education (PreK-3 age group). These early DLLs need to devote a considerable amount of time learning to speak and read the language, in order to gain the language proficiency to function and compete in the classroom. Fear of embarrassment when mispronouncing words in front of others may drive them to remain silent; effectively hampering their participation in the class and overall curricular growth. The process of learning a new language can benefit …

Developing A Kinect Based Holoportation System, Soumya Chiday

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Holographic communication and distributed collaboration offer great potential for empathic computing to help remove the cost, distance, language and expertise barriers in many social and economic activities. Recent advances in AR-enhanced communication as evident by Microsoft Holoportation technology demonstrate the progress toward fully immersive collaborations distributed and remotely. Current holoportation system requires the use of extensive camera-arrays and powerful server system due to the computation demand and sensory needs to capture and reconstruct the subject of interests Thus, they suffer in mobility and applicability in real-world scenarios. In this thesis, we present an ultra-portable holoportation system design that requires only …

Constitutive Modeling Of Creep In Leaded And Lead-Free Solder Alloys Using Constant Strain Rate Tensile Testing, Eric Thomas Stang

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Environmental and safety concerns have necessitated a phase-out of lead-based alloys, which are often used in electronics solder applications. In order to properly assess suitable replacement materials, it is necessary to understand the deformation mechanisms relevant to the application. In the case of electronics solder, creep is an important mechanism that must be considered in the design of reliable devices and systems. In this study, Power-Law and Garofalo constitutive creep models were derived for two medium temperature solder alloys. The first alloy is known by the commercial name Indalloy 236 and is a quaternary alloy of lead, antimony, tin, and …

Osmotic Activation Of Sperm Motility Via Water Flow Through Aquaporins In The Freeze-Tolerant Cope's Gray Treefrog, Dryophytes Chrysoscelis, Deja Miller

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Gametes of gray treefrogs, Dryophytes chrysoscelis, are deposited into freshwater ponds. Sperm undergo spermatogenesis and maturation beginning in the seminiferous tubules and migrating to the lumen. In mammals and fishes, these cells are immotile within the isosmotic fluid of the testes and have motility activated by exposure to a hyper- or hypoosmotic medium. Water flows into or out of the sperm cell, altering intracellular ionic concentrations, and ultimately stimulates flagellar movement. We tested the hypothesis that exposure to a hypotonic environment activates motility of gray treefrog sperm. We also hypothesized that osmotic water uptake is facilitated by expression of water …

River Biofilm Structure And Function In A Resource Landscape Modified By Agriculture: Implications For Primary Consumers, Hannah M. Fazekas

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Anthropogenic alterations to nitrogen, carbon, and phosphorus bioavailability have increased the flux of these resources into the biosphere and altered stream ecosystem function. Streams modify the transport of these resources to receiving ecosystems through uptake, transformation, and mineralization. Understanding how streams process carbon, nitrogen, and phosphorus can provide insight about how stream ecosystems function in landscapes where human modification is inescapable. I investigated how land use in agricultural regions affect resource availability to primary producers and consumers and the subsequent impact on stream processes. I surveyed headwater streams in three Lake Erie watersheds to determine spatiotemporal nutrient limitation of attached …

Very Long Engagements: The Persistent Authority Of Bridewealth In A Post-Apartheid South African Community, Michael W. Yarbrough

Publications and Research

This article examines the persistent authority of the customary practice for forming recognized marriages in many South African communities, centered on bridewealth and called “lobola.” Marriage rates have sharply fallen in South Africa, and many South Africans blame this on the difficulty of completing lobola amid intense economic strife. Using in-depth qualitative research from a village in KwaZulu-Natal, where lobola demands are the country’s highest and marriage rates its lowest, I argue that lobola’s authority survives because lay actors, and especially women, have innovated new repertoires of lobola behavior that allow them to pursue emerging needs and desires for marriage …

Oncogene Induced Dna Replication In Lung Injury Repair And Carcinogenesis, Shilpa Singh

Theses and Dissertations

Deregulation of genome duplication is a common theme in cancer cells. Although multitude of pathways has been discovered and implicated in the ability of oncogenes or loss of function tumor suppressors to induce oncogenesis, most of the pathways ultimately converge in deregulated genome duplication or uncoupling of DNA replication and segregation in cancer cells. It is important to determine how known oncogenes deregulate genome duplication, and if prevention of deregulated genome duplication could be a feasible target to thwart uncontrolled proliferation of cancer cells. Conventional paradigm ascribes the cell proliferative function of the human oncoprotein mouse double minute2 (MDM2) primarily …

Perfluorinated Alkyl Acids And Fecundity Assessment In Striped Mullet (Mugil Cephalus) At Merritt Island National Wildlife Refuge, Jacqueline T. Bangma, Jessica L. Reiner, Russell H. Lowers, Theresa M. Cantu, Jacob Scott, Jeffrey E. Korte, Doug M. Scheidt, Chris Mcdonough, Jonathan Tucker, Brenton Back, Douglas H. Adams, John A. Bowden

United States National Aeronautics and Space Administration: Publications

This study investigated wild caught striped mullet (Mugil cephalus) at Merritt Island National Wildlife Refuge (MINWR) for levels of 15 perfluoroalkyl acids (PFAA) in tandem with individual fecundity measurements (Oocyte sub-stage 2 late, n=42) and oocyte reproductive stages (Stages 1–5, n=128). PFAAmeasurementswere quantified in stripedmullet liver (n=128),muscle (n=49), and gonad (n=10). No significant negative impacts of liver PFAA burden on wild-caught,mullet fecundity endpoints were observed in this study; however, changes in PFAAwere observed in the liver asmullet progressed through different sub-stages of oocyte development. Of the PFAA with significant changes …

Rodrigo And Ressentiment: I Don't Want It If You Are Going To Get It, Too - Why Classical Economic And Political Theory Fails To Explain The Obamacare Vote, But Legal Realism And Cls Can, Richard Delgado

Articles

No abstract provided.

Legal Realism And The Controversy Over Campus Speech Codes, Richard Delgado

Articles

No abstract provided.

Picture Collection Subject Index, Fleet Library, Visual + Material Resources

Picture Collection Indexes

The Subject Files cover people, places, objects, design, fashion, buildings, nature, and concepts. Research a time period by looking at subjects that are subdivided chronologically, such as Automobiles, Costume, Family Life, Advertising, Furniture, Interiors, Houses, Street Scenes, and U.S. History. The collection covers many decades, from the 19th century to the present day, and contains many images that you won't find on the internet, and they all circulate!

"When They Enter, We All Enter": Opening The Door To Intersectional Discrimination Claims Based On Race And Disability, Alice Abrokwa

Michigan Journal of Race and Law

This Article explores the intersection of race and disability in the context of employment discrimination, arguing that people of color with disabilities can and should obtain more robust relief for their harms by asserting intersectional discrimination claims. Professor Kimberlé Crenshaw first articulated the intersectionality framework by explaining that Black women can experience a form of discrimination distinct from that experienced by White women or Black men, that is, they may face discrimination as Black women due to the intersection of their race and gender. Likewise, people of color with disabilities can experience discrimination distinct from that felt by people of …

High Heterotrophic Counts In Potable Water And Antimicrobial Resistance Among Indicator Organisms In Two Peri-Urban Communities Of Karachi, Pakistan, Sadia Shakoor, Imran Ahmed, Saima Mukhtiar, Israr Ahmed, Farzeen Hirani, Shazia Sultana, Rumina Hasan

Department of Pathology and Laboratory Medicine

Objective: Fecal contamination of potable water leads to unsafe water supply. Although many urban areas of large metropolitan cities receive safe water, peri-urban areas are often not monitored by public health authorities and water supply and quality remain unknown. We assessed microbiological quality and rates of antimicrobial resistance in viable indicator bacteria in two peri-urban communities of Karachi, Pakistan. Water samples were collected over 5 months (October 2015 to February 2016) from these peri-urban communities and samples were processed for microbiological quality as per Standing Committee of Analysts, United Kingdom and World Health Organization guidelines and criteria for drinking water. …

Workplace Standing Desks And Arterial Stiffness, Ian Greenlund

Dissertations, Master's Theses and Master's Reports

Many jobs in today’s society require sitting at a desk with little physical activity. Individuals who engage in ten hours of sedentary behavior per day double their CVD risk. Standing desks are thought to decrease sedentary time and improve cardiovascular health. Acute use of standing desks is shown to lower PWV. However, chronic effects remain unknown. Forty eight participants qualified as seated (19 females, 5 males: age 41 ± 2 years, BMI 25 ± 1 kg/m²) or standing (21 females, 3 males: age 45 ± 2 years, BMI 25 ± 1 kg/m²) groups based on habitual …

Waste Not, Want Not: Using Source-Separated Urine To Cultivate Maize In The Southern Highlands, Tanzania, Lucy Hatfield

Dissertations, Master's Theses and Master's Reports

In Sub-Saharan African countries, soil fertility is low due to unsustainable agricultural practices and little to no access to chemical fertilizer. Poor yields have resulted in many of these countries struggling to produce enough food to feed their growing populations. Human urine has been used as an effective, low-cost alternative to chemical fertilizers in greenhouse and plot trials on a variety of vegetables, but its effects on elemental grain composition in cereal crops are largely unknown. Here we tested whether diluted human urine applied as a fertilizer can increase maize crop growth, yield and nutritional content on small family-owned plots. …

Ecologies Of Sponsorship: What Fitbit Users Can Teach Us About Digital Literacy, Kimberly Tweedale

Dissertations, Master's Theses and Master's Reports

As digital technologies have expanded, so have the literacy sponsors that support and shape how those technologies are used. This project focuses on one of these growing sites of sponsorship surrounding a specific health-tracking technology: wearable Fitbit devices. While much of the work on literacy sponsorship has focused on institutional sponsors as agents, I argue that the picture becomes more complicated and interesting when we place our focus on how users—often considered the sponsored—can become agents in a system that may have marginalized, excluded, or used them.

Using a combination of qualitative methods, this dissertation highlights how various literacy sponsors …

Ecological Implications Of Foraging And Life-History In Three Cold-Adapted Species, Andrew Von Duyke

Dissertations, Master's Theses and Master's Reports

This research investigates the ecology of: ringed seals (Pusa hispida), moose (Alces alces), and bowhead whales (Balaena mysticetus). Each of these species draws upon specialized strategies to meet the physical demands of life in their extreme environments. However, they are currently experiencing unprecedented changes in their habitats. This work adds to what is known about the behavior, physiology, and population dynamics of cold-adapted species—providing insights of value for an improved understanding of foraging and life-history theory, and informing conservation and ecosystem monitoring efforts. Chapter 1 provides a brief introduction to the scope of work …

Viability Of Graywater Reuse In Lesotho’S Lowland Agricultural District, Ryan Wagner

Dissertations, Master's Theses and Master's Reports

Water security has become a large issue with populations rising and weather patterns changing to drier and hotter conditions in southern Africa. Lesotho, a mountainous country landlocked by South Africa, is responsible for a large amount of the rainfall in southern Africa. As such, Lesotho provides water to South Africa in exchange for national economic growth. Lesotho, however, is also prone to droughts and with water reserves being sold to South Africa, water security has become a national issue. This study looked at Lesotho’s current water use habits and found that graywater reuse could lead to much more available water. …

Divine Vulpine: 3d Simulation Of Colorations Of The Wild And Domesticated Red Fox, Alyssa Newsome

Senior Honors Theses and Projects

Vulpes vulpes, the red fox, has been domesticated for hundreds of years. Through the continued breeding of the red fox in captivity, there has been an explosion of variations in color, size, and temperament. With the rise of interest in domesticated foxes as pets and educational animals, there is a dire need for education of the public on these animals and their history. Fur farming also remains a lucrative industry, an industry engaged in continuous and broad academic research. Aimed at these audiences, this academic presentation allows users to view a 30 animated fox, and select its coloration, animation, view, …

Restoration Of Mauri (Life-Force) To Okahu Bay: Investigation Of The Cultural, Social, And Environmental Restoration, Emily Freilich

Pomona Senior Theses

This thesis investigated the restoration of mauri (life-force) to Ōkahu Bay, Auckland New Zealand. Ōkahu Bay is part of the land and waters of Ngāti Whātua Ōrākei, a Māori hapū (sub-tribe). Ngāti Whātua Ōrākei has been driving the restoration, restoring Ōkahu Bay based on their worldview, visions, and concerns. This vision and control of the restoration process allows them to bring in the hapū in sustainable engagement and have the long-term vision and commitment necessary for self-determination. However, while there has been progress with projects and improved decision-making authority, hapū members are still not seeing their whānau (family) swimming in …

The Long March Toward Abolition: From The Enlightment To The United Nations And The Death Penalty's Slow Demise, John D. Bessler

University of Florida Journal of Law & Public Policy

No abstract provided.

Throne Of Fans: Examining The Roles Of Feminism, Platform And Community In An Online Fandom, Sarah Leiser

Electronic Theses and Dissertations

This thesis involves examining the influence of feminism, platform and community on the fandom of Sarah J Maas (SJM) novels on the social network site Tumblr. The goals are to show how a shift to an open social network site has changed the practice of fandom and how various feminisms are applied to popular culture and learned through Tumblr. An ethnographic study and textual analysis on SJM fandom posts and interactions on Tumblr were utilized in order to reach these goals. The observations and analysis examines the data in three categories: the established characteristics of fandom on Tumblr, the characteristics …