Digital Commons Network^™

Backpack Pcr: A Point-Of-Collection Diagnostic Platform For The Rapid Detection Of Brugia Parasites In Mosquitoes, Weam I. Zaky, Francesca R. Tomaino, Nils Pilotte, Sandra J. Laney, Steven A. Williams

Biological Sciences: Faculty Publications

No abstract provided.

Altered Expression Of Phox2 Transcription Factors In The Locus Coeruleus In Major Depressive Disorder Mimicked By Chronic Stress And Corticosterone Treatment In Vivo And In Vitro, Yan Fan, Ping Chen, Muhammad U. Raza, Attila Szebeni, Katalin Szebeni, Gregory A. Ordway, Craig A. Stockmeier, Meng Yang Zhu

ETSU Faculty Works

Phox2a and Phox2b are two homeodomain transcription factors playing a pivotal role in the development of noradrenergic neurons during the embryonic period. However, their expression and function in adulthood remain to be elucidated. Using human postmortem brain tissues, rat stress models and cultured cells, this study aimed to examine the alteration of Phox2a and Phox2b expression. The results show that Phox2a and Phox2b are normally expressed in the human locus coeruleus (LC) in adulthood. Furthermore, the levels of Phox2a protein and mRNA and protein levels of Phox2b were significantly elevated in the LC of brain donors that suffered from the …

Unilateral, 3d Arm Movement Kinematics Are Encoded In Ipsilateral Human Cortex, David T. Bundy, Nicholas Szrama, Mrinal Pahwa, Eric C. Leuthardt

Open Access Publications

There is increasing evidence that the hemisphere ipsilateral to a moving limb plays a role in planning and executing movements. However, the exact relationship between cortical activity and ipsilateral limb movements is uncertain. We sought to determine whether 3D arm movement kinematics (speed, velocity, and position) could be decoded from cortical signals recorded from the hemisphere ipsilateral to the moving limb. By having invasively monitored patients perform unilateral reaches with each arm, we also compared the encoding of contralateral and ipsilateral limb kinematics from a single cortical hemisphere. In four motor-intact human patients (three male, one female) implanted with electrocorticography …

Evolution Of Bidirectional Costly Mutualism From Byproduct Consumption, Lon Chubiz, William Harcombe, Jeremy Chacón, Elizabeth Adamowicz, Christopher Marx

Biology Department Faculty Works

Mutualisms are essential for life, yet it is unclear how they arise. A two-stage process has been proposed for the evolution of mutualisms that involve exchanges of two costly resources. First, costly provisioning by one species may be selected for if that species gains a benefit from costless byproducts generated by a second species, and cooperators get disproportionate access to byproducts. Selection could then drive the second species to provide costly resources in return. Previously, a synthetic consortium evolved the first stage of this scenario: Salmonella enterica evolved costly production of methionine in exchange for costless carbon byproducts generated by …

It Is Time To Kill The Economic Theory Of Suicide, Gary N. Smith

Pomona Faculty Publications and Research

A seminal paper by Hamermesh and Soss modeled suicide as a rational economic decision based on a comparison of the financial costs and benefits of staying alive. This study investigates the theoretical and empirical validity of that model.

The Relative Effects Of Forest Amount, Forest Configuration, And Urban Matrix Quality On Forest Breeding Birds, Alexandra V. Shoffner, Andrew M. Wilson, Wenwu Tang, Sara A. Gagné

Environmental Studies Faculty Publications

Urbanization modifies landscape structure in three major ways that impact avian diversity in remnant habitat: habitat amount is reduced and habitat configuration and matrix quality are altered. The relative effects of these three components of landscape structure are relatively well-studied in agricultural landscapes, but little is known about the relative effect of urban matrix quality. We addressed this gap by investigating the relative effects of forest amount, forest configuration, and matrix quality, indicated by degree of urbanization and agriculture amount, on the diversity of three guilds of forest birds using data from 13,763 point counts from Pennsylvania, USA. Forest amount …

Lithium Cocrystal Compositions, Michael John Zaworotko, Roland D. Shytle, Tien Teng Ong, Ryan N. Cantwell, Tranhha Nguyen, Adam John Smith, Padmini Kavuru

USF Patents

2:1 cocrystals of amino acids and Li+ salts crystallize from hot water to afford water stable cationic networks based upon tetrahedral lithium cations: bilayered square grids, a lithium zeolitic metal-organic material (LiZMOM) and several lithium diamondoid metal-organic materials (LiDMOMs). The compositions may be used as a pharmaceutical for the treatment of suicidality and other disorders that require lithium to penetrate the blood brain barrier and exert therapeutic effects in the CNS. Advantageously, the novel cocrystal forms described herein may be used to lower the oral dose required to achieve therapeutic concentrations of lithium in the brain, thus reducing the peripheral …

Combination Therapy Of Stem Cell Mobilizing Agents And Stem Cell Transplantation, Cesario Venturina Borlongan, Paul R. Sanberg

USF Patents

A combined therapy of human umbilical cord blood cells (hUCB) and G-CSF at the acute stage of TBI was tested as a therapeutic for progressive secondary effects of chronic TBI. Rats were treated with saline carrier, or therapeutic in carrier as follows; G-CSF, hUCB, or hUCB and G-CSF, 7-days after TBI. Eight weeks later, behavioral testing was performed and brains harvested to analyze hippocampal cell loss, neuroinflammatory response, and neurogenesis. Results revealed that the monotherapies partially suppressed neuroinflammation and reduced hippocampal cell loss. However, combined therapy of hUCB and G-CSF robustly dampened neuroinflammation, while enhancing endogenous neurogenesis and reducing hippocampal …

A Mixed Methods Study Of The Implementation Of Collaborative Technology Tools For Enhancing Collaboration And Student Engagement In Online Learning: Faculty Experiences And Student Perspectives, Ayshah Abdullah Alahmari

Theses and Dissertations

The appropriate implementation of collaborative technology tools in online courses leads to a culture of social learning where technology empowers students to take central roles in their learning. Yet, critical questions still exist about how faculty design, develop, implement collaborative eLearning activities using technology tools that support collaboration and student engagement in online courses, and what perspectives students have toward their experiences while participating in these activities. The purpose of the study is to explore the experiences of faculty members implementing collaborative technology tools in online courses to support collaboration and student engagement, in addition, to obtain the perspectives of …

Mindfulness And Teen Behavior: Teenagers' Perceptions Of Behavior That Led To Expulsion, Michelle L. Lee

LSU Master's Theses

The purpose of this study was to examine the effects of teaching specific mindfulness techniques to teens who have difficulty functioning in the classroom, using the Mindful Schools Curricula; the course includes 18 lessons on being mindful of sound, breath, movement, thoughts, and emotions. Specifically, the study was designed to determine whether a mindfulness course would be beneficial for South Parish High School teens who are expelled. The study design was mixed methods. Participants were administered pre- and post- scientific validated survey scales that measure mindfulness characteristics as one data source. Participants’ parents were given a questionnaire to return and …

Genetic Engineering In Agriculture Curricula For Grades 9-12, Tyson Sorensen, Olivia Horning, Kelsey Hall, David Francis, Joshua Dallin

Browse all Datasets

This is a curriculum package that was developed for teachers to be able to integrate genetic engineering and biotechnology concepts, skills, and career applications into their classrooms. This package was developed as part of the LEARN workshop entitled "Genetic Engineering: Workshop for Teachers." This curriculum package includes five full units of instructions with lesson plans, presentation resources, and other resources for teachers. This package is intended for students in grades 9-12.

Each unit is complete with the corresponding slides found in the main Genetic Engineering PowerPoint. Teachers have the liberty to cover the material on an as-needed bases based on …

Miss Susie Song, Sierra Mackelprang

USU Student Folklore Fieldwork

Miss Susie had a steamboat,

The steamboat had a bell, DING DING!

Miss Susie went to heaven,

The steam boat went to-

HELLo operator please give me number nine,

And if you disconnect me,

I’ll chop of your-

BEHIND the ‘fridgerator,

There lay a piece of glass,

Miss Susie sat upon it,

And cut her little-

ASK me no more questions’

Tell me no more lies,

The boys are in the bathroom,

Zipping up their-

FLIES are in the meadow,

The bees are in the park,

Miss Susie and her boyfriend are kissing in the,

D-A-R-K

D-A-R-K

DARK! DARK! DARK! …

Independent - Nov. 20, 2018, Cecilia G Hernandez

The Independent (1988-present)

No abstract provided.

Lcp1 Mutant Zebrafish: A Look At Neutrophils, Cancer, And Gene Compensation, Taylor Mitchell

College of Science and Health Theses and Dissertations

Lymphocyte cytosolic protein 1 (lcp1 or L-plastin) is a small actin-bundling protein that is typically only expressed in motile leukocytes, such as neutrophils and macrophages. However, it is also overexpressed in cancer cells, which may be related to tumor metastasis. Using CRISPR/Cas9 gene editing, our lab has created zebrafish that are genetic knockouts for lcp1 in order to better understand the relationship between L-plastin and cell motility.

Previous studies on L-plastin knockout mice have shown that the mutants have a decreased immune response, and therefore I predicted that our zebrafish mutants might have impaired development or distribution of immune cells. …

Chronic Life Stress And Change In Stress Response Functioning In Urban Youth: The Role Of Social Support And Gender, Heather M. Marshall

College of Science and Health Theses and Dissertations

Salivary alpha-amylase (sAA) has been established as a bio-marker of stress reactivity in acute stressor tasks. Much less research exists exploring whether sAA is bio-marker for chronic stress exposure, and therefore, allostatic load. Extant research is inconsistent with some evidence to support chronic stress exposure having an impact on sAA reactivity and diurnal levels of sAA, while other studies have failed to support this theory. Social support is regarded as a protective factor against stress, mental health, and physical health consequences. It has been suggested that gender may play a role in the utilization and benefits of social support networks. …

Advances And Challenges In Gene Editing Technologies, Lu Junnan, Chu Xin, Pan Yanping, Chen Yingxi, Wen Luan, Dai Junbiao

Bulletin of Chinese Academy of Sciences (Chinese Version)

Gene editing includes genetic manipulations like deletion, replacement, insertion, etc., which aims to obtain new gene functions, phenotypes, and even new species. As a burgeoning field of life science, gene editing has brought genetic manipulation into an unprecedented grand state. Here, we summary the developments of the technologies in gene editing, and the challenges and opportunities we currently facing, which aims to deepen the comprehension of this technique systems, and help to find breakpoints of this field.

Phenotyping And Sorting Of Synthetic Cells: Building Bridge From Spectroscopy To Genetics, Ma Bo, Xu Jian

Bulletin of Chinese Academy of Sciences (Chinese Version)

In synthetic biology, the dazzling methodological innovations in sequencing, editing and synthesis of genomes have resulted in an unprecedented capability in "design and manufacturing of genotype". On the other hand, "testing of cellular phenotype and function" has increasingly become one major bottleneck. Single-cell technologies have tremendous implications and potentials in rapid testing of cellular function. However, ideally, such single-cell methods should allow non-invasive live-cell probing, be label-free, provide landscapelike phenotyping capability, distinguish complex functions, operate with high speed, sufficient throughput and low-cost, and finally, be able to couple with downstream omics analysis via cell sorting. In this perspective article, we …

Churches Pt. 10 (T-U)

Bulloch County Papers

This is the digital Churches pt. 10 folder, from the Statesboro-Bulloch County Public Library's Public History Files. The newspaper clippings that make up the folder are from the Statesboro Herald Newspaper or its predecessors.

“His Mind Will Work Better With Both Of Us”: A Qualitative Study On Fathers’ Roles And Coparenting Of Young Children In Rural Pakistan, Joshua Jeong, Saima Siyal, Günther Fink, Dana Charles Mccoy, Aisha Khizar Yousafzai

Department of Paediatrics and Child Health

Background: Parents are the primary providers of nurturing care for young children’s healthy early development. However, the literature on parenting in early childhood, especially in low- and middle-income countries, has primarily focused on mothers. In this study, we investigate how parents make meaning of fathers’ parenting roles with regards to their young children’s early health and development in rural Pakistan.
Methods: Data were collected between January and March 2017 through in-depth interviews with fathers (N = 33) and their partners (N = 32); as well as separate focus group discussions with fathers (N = 7) and mothers (N = 7). …

His Mind Will Work Better With Both Of Us”: A Qualitative Study On Fathers’ Roles And Coparenting Of Young Children In Rural Pakistan, Joshua Jeong, Saima Siyal, Günther Fink, Dana Charles Mccoy, Aisha Khizar Yousafzai

Department of Paediatrics and Child Health

Background: Parents are the primary providers of nurturing care for young children’s healthy early development. However, the literature on parenting in early childhood, especially in low- and middle-income countries, has primarily focused on mothers. In this study, we investigate how parents make meaning of fathers’ parenting roles with regards to their young children’s early health and development in rural Pakistan.
Methods: Data were collected between January and March 2017 through in-depth interviews with fathers (N = 33) and their partners (N = 32); as well as separate focus group discussions with fathers (N = 7) and mothers (N = 7). …

Nondestructive 3d Imaging And Quantification Of Hydrated Biofilm-Sediment Aggregates Using X-Ray Microcomputed Tomography., N Zhang, Cel Thompson, Ih Townend, Ke Rankin, Dm Paterson, Aj Manning

School of Biological and Marine Sciences

Biofilm-sediment aggregate (BSA) contains a high water content, either within internal pores and channels or bound by extracellular polymeric substances (EPS) forming a highly hydrated biofilm matrix. Desiccation of BSAs alters the biofilm morphology and thus the physical characteristics of porous media, such as the binding matrix within BSA and internal pore geometry. Observing BSAs in their naturally hydrated form is essential but hampered due to the lack of techniques for imaging and discerning hydrated materials. Generally, imagery techniques (scanning electron microscopy (SEM), transmission electron microscopy (TEM), and focused ion beam nanotomography (FIB-nt)) involve the desiccation of BSAs (freeze-drying or …

Metacognitive Reading Strategy And Emerging Reading Comprehension In Students With Intellectual Disabilities, Natasha Cox-Magno, Peter Ross, Kathleen Dimino, Andrea Wilson

Journal of Educational Research and Practice

This article ventures to address the gap in special education practices by providing a metacognitive reading strategy to support the emerging reading comprehension skills of kindergarten students with intellectual disabilities. Historically, students with intellectual disabilities have low reading comprehension skills that can impede their overall academic success. There is a gap in practice regarding the identification and effective use of evidence-based reading comprehension instructional strategies for students with intellectual disabilities. Guided by Piaget’s and Vygotsky’s constructivist theories, the purpose of this study was to test the effectiveness of a metacognitive reading strategy on the emerging reading comprehension (ERC) skills of …

The Ticker, November 19, 2018

The Ticker

The Ticker is the student newspaper of Baruch College. It has been published continuously since 1932, when the Baruch College campus was the School of Business and Civic Administration of the City College of New York.

Uv-C Treatment On The Safety Of Skim Milk: Effect On Microbial Inactivation And Cytotoxicity Evaluation, Danielle M. Ward, Ankit Patras, Agnes Kilonzo-Nthenge, Sudheer K. Yannam, Che Pan, Hang Xiao, Michael Sasges

Agricultural and Environmental Sciences Faculty Research

The efficacy of UV-C irradiation as a nonthermal processing method for skim milk (SM) was investigated. SM inoculated with two surrogate viruses (MS2 and T1UV), and three bacteria (Escherichia coli ATCC 25922, Salmonella enterica serovar Typhimurium ATCC 13311, and Listeria monocytogenes ATCC 19115) was treated with Deanflow UV-C irradiation, a spiral reactor with the fluid pumped around a central low-pressure mercury UV lamp (40 W) emitting at 254 nm wave-length. A series of known UV doses (0–168.33 mJ·cm²) were delivered to the samples. The microbial loads of MS2, T1UV, E. coli, Salmonella, and Listeria were …

Hopkins, Susan, Ysanne Bethel

Querying the Past: LGBTQ Maine Oral History Project Collection

Susan Hopkins is a 53-year-old member of the LGBTQIA community, living in Westbrook, Maine. Susan grew up with her family on the small island of Vinalhaven in Penobscot Bay, hearing tales of her anti-racist bisexual aunt. A self-identified feminist in her adolescence, Susan recognized that she was not straight early on, but did not feel safe to come out in her small community. Going to the University of Maine, Orono, Susan experienced her first lesbian relationship and taste of chosen family. Eventually, Susan found herself at the Howard University School of Law, where she interned at Whitman Walter Clinic in …

Relating Cyanobacteria And Physicochemical Water-Quality Properties In Willow Creek Lake, Nebraska, 2012–14, David L. Rus, Brent M. Hall, Steven A. Thomas

United States Geological Survey: Water Reports and Publications

Document abstract

Cyanobacteria (also referred to as blue-green algae) are naturally present members of phytoplankton assemblages that may detract from beneficial uses of water because some strains produce cyanotoxins that pose health hazards to people and animals. Cyanobacteria populations observed in Willow Creek Lake during 2012 through 2014 were compared to external nutrient loading from the Willow Creek drainage basin and several other physicochemical properties within the lake, including internal nutrient loading. This report is part of a cooperative study between the United States Geological Survey, the Lower Elkhorn Natural Resources District, the Nebraska Department of Environmental Quality, the Nebraska …

More Than Just Collateral Damage: Pet Shootings By Police, Courtney G. Lee

The University of New Hampshire Law Review

The Department of Justice estimates that American police officers shoot 10,000 pet dogs in the line of duty each year. It is impossible to ascertain a reliable number, however, because most law enforcement agencies do not maintain accurate records of animal killings. The tally may be substantially higher, and some suggest it could reach six figures.

Deferring to officers’ judgment when they reasonably fear for human safety is sound policy because they regularly must make split-second, life-or-death decisions in highly stressful situations; but many pet shootings occur when officers mistake the behavior of a friendly, curious dog for aggression. Further, …

Don't Go Chasing Waterfalls: The Intrepid, Pioneering, Whitewater Paddler's Right To Stop On Private Land, Jennifer Jolly-Ryan

The University of New Hampshire Law Review

The river roars from the thunder of waterfalls and swift currents. Its water runs wild between large boulders and steep banks. Kayaks and canoes are designed to navigate whitewater rapids that many people believe are unnavigable. Conflicts often arise between private property owners and the recreating public on some of the best whitewater in America. Private property owners along rivers, creeks, and streams have shot at paddlers for entering their private property. Property owners have obstructed navigation, stringing artificial strainers made of barbed wire across creeks and streams.

The sport of running wild water rapids and waterfalls in a kayak …

Semaphorin 5a Drives Melanoma Progression: Role Of Bcl-2, Mir-204 And C-Myb., Simona D'Aguanno, Elisabetta Valentini, Maria Grazia Tupone, Marianna Desideri, Marta Di Martile, Manuela Spagnuolo, Simonetta Buglioni, Cristiana Ercolani, Italia Falcone, Marco De Dominici, Michele Milella, Maria Giulia Rizzo, Bruno Calabretta, Carlo Cota, Andrea Anichini, Daniela Trisciuoglio, Donatella Del Bufalo

Department of Cancer Biology Faculty Papers

BACKGROUND: Melanoma, the most aggressive form of skin cancer, is characterized by high rates of metastasis, drug resistance and mortality. Here we investigated the role of Semaphorin 5A (Sema5A) on the properties associated with melanoma progression and the factors involved in Sema5A regulation.

METHODS: Western blotting, qRT-PCR, Chromatin immunoprecipitation (ChIP) assay, immunohistochemistry of melanoma patient specimens and xenograft tissues, in vitro Transwell assay for cell migration and invasion evaluation, in vitro capillary-like structure formation analysis.

RESULTS: A significant correlation of Sema5A mRNA expression and melanoma progression was observed by analyzing GEO profile dataset. Endogenous Sema5A protein was detected in 95% …

Casey Johnson: Professional Practices, Casey Johnson

Professional Practices (ART 399)

My art comprises one unifying idea: my own life experiences, specifically those relating to my family and friends. By depicting highly specific visual cues—such as clothing, crows, and the color blue—I combine multiple facets and memories in an attempt to communicate my feelings and experiences. Sometimes these symbols are universal; a yellow raincoat, for example, calls to mind childhood. Others are encoded within my own memories—a bird skull relates to a dream my friend had one night. My art is about more than myself; it is about finding common ground between me and my audience and exploring that overlap.

Through …