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2017

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Articles 156061 - 156090 of 160892

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Chinese Tourists' Perception For Medical Tourism Services In Bangkok, Thailand, Zhuang Liu Jan 2017

Chinese Tourists' Perception For Medical Tourism Services In Bangkok, Thailand, Zhuang Liu

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this research, a cross-sectional study, were twofold: 1) to investigate the factor structure of medical tourism perception among Chinese tourists, 2) to investigate the correlation between medical tourism perception and demographic, health characteristics among Chinese tourists. The data were analysed by performing Exploratory Factor Analysis (EFA) with varimax rotation method. The questionnaire was distributed to the samples covering 481 Chinese tourists currently travelling to Thailand through travel agency as tour group and as Free Individual Tourists (FIT). The purposive sampling technique, a non-probability sampling, was used for data collection. The research findings can be summarized as follows: …

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Pharmacognostic Specification And Chrysin Content Of Oroxylum Indicum Seed, Kirana Praserdmek Jan 2017

Pharmacognostic Specification And Chrysin Content Of Oroxylum Indicum Seed, Kirana Praserdmek

Chulalongkorn University Theses and Dissertations (Chula ETD)

Oroxylum indicum (L.) Kurz, belonging to Bignoniaceae family, is known in Thai common name as Phe-Kaa. In Thailand, it has been used in the treatment of a cough and as a purgative. However, the pharmacognostic specification of this crude drug and its chrysin content have never been established in Thailand. Consequently, this study aimed to exhibit the pharmacognostic specification, analyses of the content of chrysin and fixed oil chemical constituents of O. indicum seeds. O. indicum were collected from 15 different locations in Thailand. The results indicated that the contents of loss on drying, moisture, total ash, acid-insoluble ash, ethanol …

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A Randomized Controlled Trial On The Effectiveness Of Court-Type Traditional Thai Massage Versus Topical Diclofenac To Treat Frozen Shoulder, Puangpaka Tankitjanon Jan 2017

A Randomized Controlled Trial On The Effectiveness Of Court-Type Traditional Thai Massage Versus Topical Diclofenac To Treat Frozen Shoulder, Puangpaka Tankitjanon

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aims to evaluate the effectiveness of the court-type traditional Thai massage (CTTM) in treating patients suffering from frozen shoulder in comparison with topical diclofenac (TD). A randomized controlled trial was conducted at the Thai Traditional Medical Service Center, Sukhothai Thammatirat University, Nonthaburi province. The participants of idiopathic frozen shoulder were diagnosed by the orthopedic doctor. Sixty female patients aged were randomly assigned to receive CTTM (treatment group, n=30) and TD (control group, n=30). CTTM was performed for 12 sessions during a 1-6 week period, and followed up at week 8th, 10th. TD was administered 5 g three times …

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Macroscopic, Microscopic And Molecular Evaluations For The Identification Of Erythrina Species Distributed In Thailand, Kitipan Khaonim Jan 2017

Macroscopic, Microscopic And Molecular Evaluations For The Identification Of Erythrina Species Distributed In Thailand, Kitipan Khaonim

Chulalongkorn University Theses and Dissertations (Chula ETD)

Plants in the genus Erythrina belong to the family FABACEAE. There are six species distributed in Thailand (Erythrina fusca Lour., Erythrina stricta Roxb., Erythrina crista-galli L., Erythrina subumbrans (Hassk) Merr., Erythrina variegata L., and Erythrina indica Lam.). Due to the similarity of the morphological characters and synonym in a vernacular name, the identification of these species is ambiguous. Therefore, an accurate investigation of their identities is essential. This research aimed to distinguish six Erythrina spp. through the macroscopic, microscopic, and molecular genetic analyses. The anatomical characteristics of each species (cross section of midrib) and the constant values of leaves including …

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Effectiveness Of Healing Touch Technique Among Autistic Patient : A Randomized Control Trial, Vanvisa Sresumatchai Jan 2017

Effectiveness Of Healing Touch Technique Among Autistic Patient : A Randomized Control Trial, Vanvisa Sresumatchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

This randomized control trial study aimed to compare of effectiveness of healing touch technique in changing of behavior problem of autism patient between intervention group and control group of autistic children in Nonthaburi Autistic Center, Nonthaburi province. Data was collected by using a self-administered questionnaire from parent of autistic among 64 sampled (59 Male, 5 female) age of samples ranged from 13-26 years were included in this study. Binary logistic regression, Generalized Linear Model, and Generalized Estimating Equations were applied for the analysis.For main symptom of autistic, most of them were repetitive behavior and fixated interests 78.5% and 75.0% respectively. …

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Pharmacognostic Specification, Mangiferin Content, Biological Activities Of Aquilaria Crassna Leaves And Dna Barcodes Of Selected Plants In Genus Aquilaria, Woratouch Thitikornpong Jan 2017

Pharmacognostic Specification, Mangiferin Content, Biological Activities Of Aquilaria Crassna Leaves And Dna Barcodes Of Selected Plants In Genus Aquilaria, Woratouch Thitikornpong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aquilaria crassna Pierre ex Lecomte (Thymelaeaceae) has been used as a medicinal plant in many aspects. Previous research has revealed that A. crassna leaves contain mangiferin as an active compound. Although the active component has been investigated, the pharmacognostic specification and quantification of mangiferin from A. crassna leaves have never been established. There still have little findings about biological activities of A. crassna leaves extract and its metabolite, mangiferin as well as the molecular evaluation of plant in genus Aquilaria. The study aimed to conduct and develop a pharmacognostic standard according to WHO guidance as well as the validated method …

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Plan Member’S Heterogeneity, Economic Regime Effect And Their Implication On The Management And Sustainability Of Retirement Funds, Thepdanai Danswasvong Jan 2017

Plan Member’S Heterogeneity, Economic Regime Effect And Their Implication On The Management And Sustainability Of Retirement Funds, Thepdanai Danswasvong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research studies the importance of plan members' heterogeneity to the management of defined benefit (DB) pension fund. We propose a new multi-member model of DB pension fund that allows for heterogeneity in plan members' retirement ages, salary growths, and other characteristics. We first solve analytically for optimal management strategy and show that the sponsor's supplementary contribution and the fund's allocation in risky assets are determined by the cross-product between the fund's expected retirement liabilities and some heterogeneity-adjusted discount factors. We then demonstrate that the presence of heterogeneity can have a significant influence on the optimal management strategy and that …

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Standardization Of Chromolaena Odorata, Kaempferia Parviflora And Zanthoxylum Piperitum, Yamon Pitakpawasutthi Jan 2017

Standardization Of Chromolaena Odorata, Kaempferia Parviflora And Zanthoxylum Piperitum, Yamon Pitakpawasutthi

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chromolaena odorata (L.) R.M. King & H. Rob. or Sabsue is a species in family Asteraceae. In Thailand, it is locally used to treat skin diseases, diabetes, as well as insect bites. Kaempferia parviflora Wall. ex Baker or Krachai Dum belongs to Zingiberaceae family. In herbal medicine, it is generally used to promote health, to cure gastrointestinal disorder and anti-inflammation. Zanthoxylum piperitum (L.) DC., belonging to the Rutaceae family, is known in Thai name as Prig Horm. All parts of Z. piperitum are used to treat abdominal pain, vomiting and diarrhea. Chlorogenic acid, 5,7-dimethoxyflavone and xanthoxylin are the active components …

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Structural Model Of Engagement And Turnover Intention In Thai Pharmacist, Tulakarn Nakpun Jan 2017

Structural Model Of Engagement And Turnover Intention In Thai Pharmacist, Tulakarn Nakpun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Turnover of community pharmacist can affect continuity of patient care and most importantly the quality of care and also the costs of a pharmacy. It was hypothesized that organizational resources, job characteristics, and social supports had direct effect on pharmacist turnover intention, and indirect effect on pharmacist turnover intention via pharmacist engagement. This research aimed to study influencing factors on pharmacist engagement and pharmacist turnover intention by testing the proposed structural hypothesized model to explain the relationship among organizational resources, job characteristics, and social supports that effect on pharmacist turnover intention and pharmacist engagement in Thai community pharmacists. A cross …

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The Fall Of The Communist Party Of Thailand From The Chinese Perspective, Feng Cui Jan 2017

The Fall Of The Communist Party Of Thailand From The Chinese Perspective, Feng Cui

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Communist movement in Thailand was part of the international communist movement. So Communism in Thailand was introduced from outside and also influenced by the outside world. The history of Communist Party of Thailand (CPT) was part of Cold War. In fact, the rise of the CPT and China's help were inseparably intertwined. Thai communists could not develop so fast without the help of China after the 1960s. However, the CPT finally failed in the 1980s due to its wrong strategy of development, internal divisions and struggles, the government's counterinsurgency, the loss of support from China, among other factors. There …

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A New Multilateral Electricity Trading Model For Asean, Kevin Mark Lee Jan 2017

A New Multilateral Electricity Trading Model For Asean, Kevin Mark Lee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The ASEAN Power Grid (APG) was initiated in 1997 as a means to achieve energy security in Southeast Asia in a sustainable manner. Two decades on, the APG project faces significant challenges to the development of both physical and institutional infrastructure. Many of these cannot be easily addressed due to the socio-political realities of the region. The ASEAN electricity exchange initiative (AEE), a proposal by the ASEAN Energy Market Initiative, may prove to be a more feasible approach for regional power sector integration, as it does not require complete domestic electricity market unbundling or privatization. This thesis analyzes regional electricity …

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Spelling Errors In Thai Made By Chinese And Lao Students Speaking Thai As A Foreign Language, Peng Hou Jan 2017

Spelling Errors In Thai Made By Chinese And Lao Students Speaking Thai As A Foreign Language, Peng Hou

Chulalongkorn University Theses and Dissertations (Chula ETD)

When learning a foreign language, it is important to learn how to accurately spell as it is crucial for communication. To accurately spell in the Thai language is challenging for both native and foreign learners of Thai. However, there are few studies that address the spelling errors made by foreign learners of Thai. Therefore, the purpose of this study is to analyze the patterns and causes of spelling errors made by Chinese and Lao students speaking Thai as a foreign language. In order to gather data for this analysis, thirty Chinese students and thirty Lao students took part in a …

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Factors Influencing Chinese Medical Tourists' Satisfaction With Medical Services In Thailand, Xiyuan Zhao Jan 2017

Factors Influencing Chinese Medical Tourists' Satisfaction With Medical Services In Thailand, Xiyuan Zhao

Chulalongkorn University Theses and Dissertations (Chula ETD)

Medical tourism is a new worldwide niche market, which generates apparent economic benefits. Chinese medical tourists are a significant consumer group in the medical tourism industry that many countries have begun to explore. This research aims to study the factors motivating mainland Chinese medical tourists to seek healthcare in Thailand and to assess their satisfaction with the medical services they received in this country. This research adopted both qualitative and quantitative methods. The findings indicate that the key factors motivating mainland Chinese medical tourists to travel to Thailand to receive in medical services include recommendations and media advertisements, national policies, …

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The Role Of Chinese Commercial Associations In Supporting New Chinese Sojourners In Bangkok : A Case Study Of The Thai-Guangxi Commercial Association, Zhong Jin Wen Jan 2017

The Role Of Chinese Commercial Associations In Supporting New Chinese Sojourners In Bangkok : A Case Study Of The Thai-Guangxi Commercial Association, Zhong Jin Wen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Thailand has always maintained friendly relations with China throughout long history, which include relationship in the cultural, political, economic, social and tourism areas, among others. In recent decades, especially following the development of the Chinese "one belt, one road" policy, more and more Chinese sojourners have chosen Thailand as their sojourning destination. However, looking back to the history, we will see that most old Chinese immigrants moved from China to Thailand to make a living as businessmen or labor force. During that period, commercial associations needed to be established, so that these Chinese immigrants could help each other, (such an …

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The Effects Of Digital Story Writing Instruction On Narrative Writing Skills Of Junior High School Students In Indonesia, Elsa Widya Hapsari Jan 2017

The Effects Of Digital Story Writing Instruction On Narrative Writing Skills Of Junior High School Students In Indonesia, Elsa Widya Hapsari

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of the study were: 1) to explore the effects of digital story writing instruction on narrative writing skills of junior high school students in Indonesia; 2) to investigate how digital story writing instruction enhance English writing motivation of junior high school students in Indonesia. The instruments used in this study were online narrative writing test, writing motivation questionnaire, and a semi-structured interview. The sample in this study was 35 students from eighth- grade who were studying in 2017 academic year at Sekolah Menengah Pertama Islam Terpadu (SMP IT) in Indonesia. The research design was one-group pretest-posttest, quasi-experimental design. …

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Students’ Motivation And Teachers’ Motivational Strategies In English Language Classrooms In Unstable Socio-Political Contexts, Hkawn San Nra Jan 2017

Students’ Motivation And Teachers’ Motivational Strategies In English Language Classrooms In Unstable Socio-Political Contexts, Hkawn San Nra

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigated students' motivation, teachers' use of motivational strategies in English classrooms in an unstable socio-political state in Myanmar. In addition, how the situation affected the use of motivational strategies was also explored. Since previous studies have shown how students and teachers in such areas may be affected by the situation, this study will provide insights into how teacher education institutions can prepare and support the teachers in such areas. Sequential explanatory mix-method was employed and the data were collected from 172 students and 5 teachers in eleventh grade English classrooms from five upper secondary schools. Questionnaires, classroom observations, …

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Effects Of Direct Vocabulary And Vocabulary Learning Strategies Instruction On Indonesian Prospective English Teachers’ Vocabulary Size, Sudarman - Jan 2017

Effects Of Direct Vocabulary And Vocabulary Learning Strategies Instruction On Indonesian Prospective English Teachers’ Vocabulary Size, Sudarman -

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of the study were: 1) to explore the effects of direct vocabulary and vocabulary learning strategies instruction on Indonesian prospective English teachers' vocabulary size, and 2) to investigate the students' opinions towards the treatment of direct vocabulary and vocabulary learning strategies instruction. The study was one-group pretest-posttest, pre-experimental design. The instruments employed in this study were the Vocabulary Levels Test (VLT) and a semi-structured interview. The samples were the 33 first-year students majoring in English education at University of Kutai Kartanegara, Indonesia for the Academic Year 2017/2018. The findings of the study showed that there was a significant …

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The Development Of The English Content-Based Reading Materials For Buddhist Student Monks, Phatchareporn Supphipat Jan 2017

The Development Of The English Content-Based Reading Materials For Buddhist Student Monks, Phatchareporn Supphipat

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this study are 1) to develop the English content-based reading materials for Buddhist student monks, 2) to examine the attitude of student monks towards the quality of the English content-based reading materials, and 3) to examine the attitude of teachers towards the quality of the English content-based reading materials. The study was conducted in 6 stages starting from the identification of needs to create materials to the evaluation of the effectiveness of the developed materials. The participants of 42 student monks and 3 English teachers were purposefully selected from the Education Department of Wat Phra Dhammakaya in …

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Effects Of Holistic Approach Using Local Cultural Content On English Oral Communication Ability Of Sixth Grade Students In Phrae Province, Wasida Sawongta Jan 2017

Effects Of Holistic Approach Using Local Cultural Content On English Oral Communication Ability Of Sixth Grade Students In Phrae Province, Wasida Sawongta

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to study the effects of Holistic Approach using local cultural content on English oral communication ability of sixth grade students and to investigate their opinions towards learning through Holistic Approach using local cultural content. The participants were 21 sixth grade students studying in Phrae Province in academic year 2017. The quantitative and qualitative data were collected in 13-week experiment using seven instruments including need analysis questionnaire, English oral communication test, English oral communication scoring rubrics, lesson plans, self-evaluation form, Metacognitive Awareness Inventory questionnaire, and focus group discussion. The Wilcoxon Signed Ranks Test, mean scores, and standard deviation …

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An Exploration Of Motivational Strategies, Beliefs, And Contextual Factors: A Case Of Chinese Efl Teachers, Xiaosu Chen Jan 2017

An Exploration Of Motivational Strategies, Beliefs, And Contextual Factors: A Case Of Chinese Efl Teachers, Xiaosu Chen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Based on the Self-determination theory, teachers can enhance learners' intrinsic motivation and their use of motivational strategies may be influenced by their beliefs and contextual factors. This case study was thus designed to explore these relationships in the contexts of EFL classrooms in Northwest of China, in which student's motivation levels in English learning were found in previous studies to be lower than that of the students in other regions. Six EFL teachers from a public upper secondary school in this region were observed in their tenth grade English classrooms over one month. After the observations, they were interviewed to …

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Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak Jan 2017

Difference Of Some Spermatozoa And Seminal Plasma Proteins In Good And Poor Freezability Boar Ejaculates, Janyaporn Rungruangsak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The present study was performed to determine the differences of some seminal plasma proteins, i.e., glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), and some spermatozoa proteins, i.e., triosephosphate isomerase (TPI) and ACRBP (acrosin binding protein), in the boar semen having good and poor freezability. The study was conducted by including 38 ejaculates in seminal plasma study and 32 ejaculates from spermatozoa study from 27 boars (Duroc=13, Landrace= 8, Yorkshire= 6). The semen was split into two portions. The first portion was centrifuged to separate the pellet of sperm from the seminal plasma. The samples were kept at −80 °C …

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The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart Jan 2017

The Effects Of Hydroxyethyl Starch (Hes) On Quality Of Equine Sperm Following Cold Storage And Cryopreservation, Ornwanya Nuchanart

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed at examining the effects of different concentrations of hydroxyethyl starch (HES) on quality of equine sperm following cold storage and cryopreservation. In experiment 1, the semen was collected from 6 stallions (3 replicates). The semen was diluted with extender for cold storage containing different concentrations of HES (2.5%, 5%, 10% and 15%). Extender without HES (0% HES) served as a control group. The temperature of extended semen was gradually decreased to 4 ºC. The quality of sperm in terms of progressive motility and viability was evaluated after cold storage for 6, 24 and 48 hours. This study …

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The Foundation For The Promotion Of Supplementary Occupations And Related Techniques Of Her Majesty Queen Sirikit And The Enhancement Of Human Securityin Northeast Thailand During The 1970s-1980s, Dollaya Tiantong Jan 2017

The Foundation For The Promotion Of Supplementary Occupations And Related Techniques Of Her Majesty Queen Sirikit And The Enhancement Of Human Securityin Northeast Thailand During The 1970s-1980s, Dollaya Tiantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation aims to launch an approach in assessing the role played by the SUPPORT Foundation in relation to enhancement of human security and to disseminate Her Majesty Queen Sirikit's role in enhancing human security in Thai society. The objectives are to study the process and activities of the SUPPORT Foundation in the northeastern provinces - Nakhon Phanom, Sakon Nakhon, Kalasin and Mahasarakham - during the 1970s -1980s and to analyze the roles and the implication of the SUPPORT Foundation in enhancing human security. The study indicates the aims of promoting local handicrafts as a supplementary occupation amongst Thais who …

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Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee Jan 2017

Factors Influencing Colostrum Consumption Of Piglets And Levels Of Immunoglobulin G And Immunoglobulin A Specifically Against Ped Virus In Colostrum Of Sows, Patthawan Juthamanee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aims of the present study was to investigate factors associated with colostrum consumption of the neonatal piglets and determine the concentrations of total immunoglobulins G (IgG) and immunoglobulins A (IgA) against porcine epidemic diarrhea (PED) virus in colostrum for sow. The study consisted of two parts: Part I, the study included data of colostrum consumption from 1,140 neonatal piglets from 80 sows. Factors associated with piglet’s colostrum consumption included body weight at birth of the piglet, birth order, birth interval, heart rate, blood oxygen saturation, rectal temperature at 24 h, gestation length, total born, born alive, sow body conditions …

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Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan Jan 2017

Establishment Of Cardiac Lineage From Rabbit Embryonic And Induced Pluripotent Stem Cells, Praopilas Phakdeedindan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Rabbit is a promising model for studying myocardial infarction as several physiological and pathological conditions are resemble to humans. However, the information on establishment of rabbit pluripotent stem cells (PSC) and differentiation potential especially cardiac differentiation in this species has been limited. This research aimed at establishing and examining in vitro cardiogenesis of rabbit embryonic stem cells (ESC) and rabbit induced pluripotent stem cells (iPSC). In experiment 1, four cell lines of ESC derived from blastocyst stage embryos were cultured and characterized for their pluripotency in terms of gene and immunohistochemistry. They exhibited normal karyotypes (n=44). Embryoid body (EB) formation …

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Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun Jan 2017

Development Of Inactivated Mycoplasma Gallisepticum Vaccine In Chickens, Arithat Limsatanun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mycoplasma gallisepticum (MG) is the respiratory pathogen causing chronic respiratory disease (CRD) in poultry. More importantly, MG infection affects the economic losses of poultry industry due to decrease egg production and carcass quality. There are several procedures for MG monitoring and several commercial vaccines to prevent and control the MG infection. In Thailand, MG infection has been the one of major problems of poultry industry. The objectives of this study were to investigate the different molecular techniques for MG monitoring in the poultry farms and to prepare the inactivated vaccine or bacterin with Thai MG strain. There were 3 experiments …

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Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan Jan 2017

Comparative Study Of Urinary N-Acetyl-Beta-D-Glucosaminidase Levels, Urinary Glycosaminoglycans And Plasma Glycosaminoglycans Levels In Cats With Feline Idiopathic Cystitis, Jeeranan Benjasiriwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Comparative study was conducted to measure urinary N-acetyl-β-glucosaminidase (NAG), urinary glycosaminoglycans (GAGs) and plasma glycosaminoglycans (GAGs) in cats with feline idiopathic cystitis (FIC). A standard questionnaire was designed to gather information for all cats including signalment, characteristics, environment, type of food and management of the cats’ litter box to evaluate the risk factors for developing FIC. Blood and urine samples were collected from 19 clinically normal cats and 19 aged and sex matched cats with FIC. Concentration of urinary NAG, urinary GAGs and plasma GAGs were measured by colorimetric method. NAG index and GAGs-to-creatinine ratio were calculated. The results demonstrated …

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The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul Jan 2017

The Role Of Triarylmethane-34 On Potassium Channel Kca3.1 In Doxorubicin-Induced Feline Kidney Cell Line, Paralee Nantarakchaikul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Study involved with potassium channel KCa3.1 blocker (triarylmethane-34; TRAM-34) in doxorubicin (DOX)-induced feline kidney cells was performed. The study was divided into two parts. In part I, subtoxic dose of TRAM-34 in feline kidney cell lines was studied to determine the cytotoxicity of TRAM-34 in feline kidney cells by cytotoxicity assay. In part II, feline kidney cell lines were incubated with appropriate dose and time of TRAM-34 pretreatment for 24 h or 30 min and post-treatment for 24 h condition in DOX-induced cell toxicity. Cytotoxicity assay, apoptosis and necrosis assay and KCa3.1 protein expression were measured. The cytotoxicity results indicated …

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Serological Status Of Pcv2-Infected Swine Herds, Cherdpong Phupolphan Jan 2017

Serological Status Of Pcv2-Infected Swine Herds, Cherdpong Phupolphan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to determine the dynamics of serological and virological profiles against Porcine Circovirus type 2 (PCV2) infection in Thai swine herds using the in-house indirect ELISA and real-time PCR techniques. A total of 10 swine herds in Thailand were divided into two groups according to their clinical history. Group A (n=5) had clinical signs of PCV2, and was routinely vaccinated by PCV2 vaccines with production loss more than 5%. Group B (n=5) had no clinical signs of PCV2 with production loss less than 5%. Group B consisted of two subgroups: PCV2-vaccinated herds (B-Vac, n=2) and non-vaccinated herds (B-non-Vac, …

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Development Of Dna Vaccine Against Porcine Epidemic Diarrhea Virus, Gun Temeeyasen Jan 2017

Development Of Dna Vaccine Against Porcine Epidemic Diarrhea Virus, Gun Temeeyasen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea (PED) is a devastating enteric disease mainly affected to the sucking piglets which causing the severe economic losses in many countries worldwide including Thailand. To date, there have no satisfied commercial vaccine for PED. The clustering system based on the spike gene demonstrated 2 variant of PEDV, classical and pandemic variant, which the difference of these variants may be affected to the protective immunity between them. The objectives of this study were to investigate the genetic diversity of S gene of PEDV in Thailand and evaluate the efficacy of DNA vaccine which developed from Thai PEDV isolate. …

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