Digital Commons Network^™

Effects Of Filtration Conditions On Clearance Of Bacteriophage, Rigen Te

Graduate Theses and Dissertations

Virus Clearance is one of the major challenges in biopharmaceutical industry, especially in the manufacturing of drugs. U.S. Food and Drug Administration (FDA) recommends using several model bacteriophages as surrogates of pathogenic mammalian virus for process validation. In this work, two bacteriophages were tested against 30 kDa, 100 kDa, 300 kDa polyethersulfone (PES) membranes to investigate the effects of pore size on virus clearance. Virus particles were spiked into the protein feed solutions containing bovine serum albumin (BSA) or lysozyme at different concentrations. Besides protein concentration, the effects of feed pH on the filtration performance and virus rejection were also …

The Parent/Teacher Relationship And The Effectiveness Of The Teaching Important Parenting Skills (Tips) Program For Enhancing Parents' Knowledge About Child Development, Meghan Anderson

Graduate Theses and Dissertations

Parent involvement in education has been highly encouraged because of its strong associations with positive developmental outcomes for children. Teaching Important Parenting Skills: TIPS for Great Kids! (TIPS) is a program in Arkansas that relays research-based information cards to parents through the support network of the teachers. This study examines how effective the TIPS program was in enhancing parent’s knowledge on child development and observes the influence of the parent-teacher relationship. Although no significant correlation was found between the parent/teacher relationship and parent knowledge, a significant correlation was discovered between the parent/teacher relationship and whether parents read the TIPS card …

A Numerical Study Of The Interaction Between One Dimensional Carbyne Chain And Single Stranded Dna, Zeina Salman

Graduate Theses and Dissertations

resolution at the single nucleotide level when developing DNA sequencers. The purpose of this research was to numerically study the electrical properties associated with the interaction between 1D carbon chain, known as carbyne, and ssDNA. First, the electrical properties of the carbyne chain were calculated. Second, the electrical properties of the carbyne chain were calculated in the presence of different ssDNA bases. Analyzing the differences between the two cases led to determining the effects of these different bases on the electrical properties. The numerical simulation approach conducted in this research was based on the first-principle simulation. The first-principle simulation was …

2017 August, Office Of Communications & Marketing, Morehead State University.

Morehead State Press Release Archive, 1961 to the Present

Press releases for August of 2017.

Distributions, Sources, And Transformations Of Dissolved And Particulate Iron On The Ross Sea Continental Shelf During Summer, Chris M. Marsay, Pamela M. Barrett, Dennis J. Mcgillicuddy Jr., Peter N. Sedwick

OES Faculty Publications

We report water column dissolved iron (dFe) and particulate iron (pFe) concentrations from 50 stations sampled across the Ross Sea during austral summer (January-February) of 2012. Concentrations of dFe and pFe were measured in each of the major Ross Sea water masses, including the Ice Shelf Water and off-shelf Circumpolar Deep Water. Despite significant lateral variations in hydrography, macronutrient depletion, and primary productivity across several different regions on the continental shelf, dFe concentrations were consistently low (<0.1 nM) in surface waters, with only a handful of stations showing elevated concentrations (0.20-0.45 nM) in areas of melting sea ice and near the Franklin Island platform. Across the study region, pFe associated with suspended biogenic material approximately doubled the inventory of bioavailable iron in surface waters. Our data reveal that the majority of the summertime iron inventory in the Ross Sea resides in dense shelf waters, with highest concentrations within 50 m of the seafloor. Higher dFe concentrations near the seafloor are accompanied by an increased contribution to pFe from authigenic and/or scavenged iron. Particulate manganese is also influenced by sediment resuspension near the seafloor but, unlike pFe, is increasingly associated with authigenic material higher in the water column. Together, these results suggest that following depletion of the dFe derived from wintertime convective mixing and sea ice melt, recycling of pFe in the upper water column plays an important role in sustaining the summertime phytoplankton bloom in the Ross Sea polynya.

Cellulose Reinforced Thermoplastic Composites By In-Situ Ring-Opening Polymerization, Shahab Kashani Rahimi

Dissertations

Over the past two decades, the increasing concern about the negative environmental impacts of synthetic materials has led to rising interests in utilizing renewable natural resources to develop polymer materials with comparable properties and performance to their synthetic counterparts. One of the major fields of interest is polymer composites where the replacement of synthetic fibers with bio renewable natural fibers is of great potential. However, the processing difficulties, in terms of fiber dispersion and thermal stability have limited the application of cellulosic fibers to polymers with low processing temperatures which are mostly hydrophobic polymers. As a result, the true reinforcing …

The Effect Of Instructional Strategies On Math Anxiety And Achievement: A Mixed Methods Study Of Preservice Elementary Teachers, Janelle K. Lorenzen

Dissertations

This study addressed how different instructional strategies affected preservice elementary teachers’ levels of math anxiety and their achievement in a math content course while considering descriptions of their experiences in the course in relation to their math anxiety and achievement. The instructional strategies used were traditional teaching methods and inquiry-based learning (IBL). A mixed methods embedded design was used in which the major design of the study is a nonequivalent control group design, where the collection of data occurred before, during, and after the intervention. There were 103 participants who were elementary education preservice teachers with 58 of them being …

A Multi-Indicator Assessment Of Conservation Success Across A Populated Forest Anthrome, Emma Cook, Natalie Ribble, John Quinn

Biology Presentations

The heterogeneous landscape of the eastern United States has resulted in a diversity of conservation interventions including public protected areas, private lands held in easements, and open space managed at various levels of intensity. Given the pressures of expected land use and land cover change, particularly in the Southern Piedmont ecoregion, multi-scale and comprehensive data are needed to prioritize conservation actions. We assessed local and regional indicators of conservation success across an urban-rural gradient by analyzing local bird abundance and diversity as a factor of forest patch area, land use matrix, and land management strategy. The study was conducted across …

The Introductory Geoscience Experience: A Study Of Undergraduate Attitudes And Engagement At Suny Buffalo State, Heather J. Mccarthy

Multidisciplinary Studies Theses

Educational priorities in STEM at the undergraduate level include sparking leadership and innovation in emerging and rapidly changing fields and educating a scientifically literate populace. These priorities depend on the nature and quality of the undergraduate educational experience (NSF IUSE Program, 2015). This study focuses on investigating student attitudes and department engagement with the resulting data offering a snapshot of student interests, values, and resource awareness.

During the Fall 2016 and Spring 2017 semesters, 359 students studying Introductory Geology, Introductory Geology Lab, Introductory Astronomy, or The Solar System and two professors who taught those classes were asked to take part …

Drip Irrigation For Commercial Vegetable And Fruit Production, Tiffany Maughn, Niel Allen, Dan Drost

All Current Publications

Drip irrigation is a highly efficient irrigation method well suited to many fruit and vegetable row crops. Drip tubing or tape discharges water to the soil through emitters positioned close to the plant. The drip tubing can be placed uncovered on the soil surface, under plastic mulch, buried in the soil, or suspended above the ground (e.g., on a trellis system). Water application rate is relatively low and irrigations are usually frequent. Properly designed and maintained drip-irrigation systems can have benefits that help increase the profitability of crop production.

On-Chip Detection Of Gel Transition Temperature Using A Novel Micro-Thermomechanical Method, Tsenguun Byambadorj, Erfan Dashtimoghadam, Mohamadali Malakoutian, Benyamin Davaji, Lobat Tayebi, James E. Richie, Chung-Hoon Lee

Electrical and Computer Engineering Faculty Research and Publications

We present a new thermomechanical method and a platform to measure the phase transition temperature at microscale. A thin film metal sensor on a membrane simultaneously measures both temperature and mechanical strain of the sample during heating and cooling cycles. This thermomechanical principle of operation is described in detail. Physical hydrogel samples are prepared as a disc-shaped gels (200 μm thick and 1 mm diameter) and placed between an on-chip heater and sensor devices. The sol-gel transition temperature of gelatin solution at various concentrations, used as a model physical hydrogel, shows less than 3% deviation from in-depth rheological results. …

The Effects Of Frondoside A In Acute Leukemia, Fatma Hussain Sajwani

Theses

Among hematological malignancies, acute leukemia is the major cause of mortality. Despite improvement of survival with current chemotherapies, some patients still diefrom the disease or the treatment side effects. Thus, new therapeutic agents are needed. Anti-cancer drugs derived from natural products are of interest. Frondoside A is a triterpenoid glycoside form the sea cucumber, Cucumaria frondosa that has shown potent antitumor effects in various cancers. Previous studies in acute leukemia are limited. The current study investigated the effects of frondoside a in acute leukemia cell lines alone and in combination with drugs currently used for this malignancy. This study is …

The Behavioral Effects Of Feeding Enrichment On A Zoo-Housed Herd Of African Elephants (Loxodonta Africana), Caroline Marie Driscoll

Graduate Theses

A comprehensive study on the behavioral effects of feeding enrichment was conducted on six African elephants housed at the North Carolina Zoological Park in Asheboro, NC. The herd is comprised of are two adult males, three adult females, and one subadult female. The study was conducted over a 10-month period and consisted of focal sample observations across three conditions. Observations were recorded during the baseline condition (June to September) and continued through the introduction of feeding enrichment. Behavioral data were also collected when enrichment items were present but food presentation followed the historical husbandry routine, known as the experimental control …

Impact Of Salinity Change On Water Quality Variables From The Sediment Of An Artificial Lake Under Anaerobic Conditions, Tae-Hoon Kim, James Jihoon Kang, Sang Hyun Kim, I-Song Choi, Kwang-Hyeon Chang, Jong Min Oh, Ki-Hyun Kim

School of Earth, Environmental, & Marine Sciences Faculty Publications

Artificial reservoirs are created by dike construction in coastal areas to secure the agricultural water supply on the west coast of South Korea. These systems are found to retain brackish characteristics until total desalination is achieved. To evaluate the effects of salinity on the organic substances released from the sediment, a laboratory water-sediment column experiment was conducted to assess associated changes in water quality variables such as the sediment oxygen demand (SOD), chemical oxygen demand (COD), and concentrations of nitrogen (N) and phosphorus (P). Under high salinity conditions, the release of N from the sediment was promoted with the enhancement …

Bioleaching Of Pyrite By Iron-Oxidizing Acidophiles Under The Influence Of Reactive Oxygen Species, D Huynh, S Bellenberg, M Vera Véliz, A Poetsch, W Sand

School of Biomedical Sciences

After 24h of exposure to acidic media, pyrite generates reactive oxygen species (ROS). Freshly-crushed pyrite with grain sizes between 50-100 µm at a 5 % (w/v), pulp density generated 0.17 ± 0.01 mM H2O2, while 10% pyrite generated 0.29 ± 0.01 mM and 30 % pyrite generated approximately 0.83 ± 0.06 mM. These levels of H2O2 inhibit iron oxidation in iron-grown cells of Acidithiobacillus ferrooxidansT but not in pyrite-grown cells. ROS originating from pyrite, which was incubated for 24 h in acidic medium, prohibited pyrite dissolution by iron-grown cells, while pyrite-grown cells were adapted to these concentrations of ROS. Periodical …

Two Distinct Mechanisms Govern Rpos-Mediated Repression Of Tick-Phase Genes During Mammalian Host Adaptation By Borrelia Burgdorferi, The Lyme Disease Spirochete, A Grove, Dionysios Liveris, Radha Iyer, Mary Petzke, J Rudman, M Caimano, J Radolf, Ira Schwartz

NYMC Faculty Publications

The alternative sigma factor RpoS plays a key role modulating gene expression in Borrelia burgdorferi, the Lyme disease spirochete, by transcribing mammalian host-phase genes and repressing sigma(70)-dependent genes required within the arthropod vector. To identify cis regulatory elements involved in RpoS-dependent repression, we analyzed green fluorescent protein (GFP) transcriptional reporters containing portions of the upstream regions of the prototypical tick-phase genes ospAB, the glp operon, and bba74 As RpoS-mediated repression occurs only following mammalian host adaptation, strains containing the reporters were grown in dialysis membrane chambers (DMCs) implanted into the peritoneal cavities of rats. Wild-type spirochetes harboring ospAB- and glp-gfp …

Support With Fathering An Adolescent Daughter: A Heuristic Investigation Of Men's Experiences With A Targeted Fathering Intervention, Jeffrey Boatner

Doctoral Dissertations and Projects

This heuristic study explored and described the experiences of fathers who participated in an intervention designed to promote and improve their relationships with their adolescent daughter(s). Despite increasing interest in the distinct contribution that fathers make to the development and well-being of their children, the father-daughter relationship remains the least studied and least understood of all parent-child pairings. Paternal involvement is a powerfully protective influence for girls—particularly as girls enter into adolescence and prepare for adulthood—yet there remain few interventions that are specifically designed to support fathers at this critical time. This study examined the experiences of 11 fathers who …

Magnetic Control Of Lateral Migration Of Ellipsoidal Microparticles In Microscale Flows, R. Zhou, C. A. Sobecki, J. Zhang, Yanzhi Zhang, Cheng Wang

Mathematics and Statistics Faculty Research & Creative Works

No abstract provided.

Application Of Open-Access Databases To Determine Functional Connectivity Between Resveratrol-Binding Protein Qr2 And Colorectal Carcinoma, Barbara B. Doonan, Evelien Schaafsma, John T. Pinto, Joseph M. Wu, Tze-Chen Hsieh

NYMC Faculty Publications

Colorectal cancer (CRC) is a major cause of cancer-associated deaths worldwide. Recently, oral administration of resveratrol (trans-3,5,4'-trihydroxystilbene) has been reported to significantly reduce tumor proliferation in colorectal cancer patients, however, with little specific information on functional connections. The pathogenesis and development of colorectal cancer is a multistep process that can be categorized using three phenotypic pathways, respectively, chromosome instability (CIN), microsatellite instability (MSI), and CpG island methylator (CIMP). Targets of resveratrol, including a high-affinity binding protein, quinone reductase 2 (QR2), have been identified with little information on disease association. We hypothesize that the relationship between resveratrol and different CRC etiologies …

The Evolution Of Natriuretic Peptide Augmentation In Management Of Heart Failure And The Role Of Sacubitril/Valsartan, Srikanth Yandrapalli, Wilbert Aronow, P Mondal, D Chabbott

NYMC Faculty Publications

Heart failure (HF) is one of the leading causes of morbidity, mortality, and health care expenditures in the US and worldwide. For three decades, the pillars of treatment of HF with reduced ejection fraction (HFrEF) were medications that targeted the sympathetic nervous system (SNS) and the renin-angiotensin-aldosterone system (RAAS). Prior attempts to augment the natriuretic peptide system (NPS) for the management of HF failed either due to lack of significant clinical benefit or due to the unacceptable side effect profile. This review article will discuss the NPS, the failure of early drugs which targeted the NPS as therapies for HF, …

Delayed Care And Mortality Among Women And Men With Myocardial Infarction, R Bugiardini, B Ricci, E Cenko, Z Vasiljevic, S Kedev, G Davidovic, M Zdravkovic, D Milicic, M Dilic, O Manfrini, Akos Koller, L Badimon

NYMC Faculty Publications

Background-Women with ST-segment-elevation myocardial infarction (STEMI) have higher mortality rates than men. We investigated whether sex-related differences in timely access to care among STEMI patients may be a factor associated with excess risk of early mortality in women. Methods and Results-We identified 6022 STEMI patients who had information on time of symptom onset to time of hospital presentation at 41 hospitals participating in the ISACS-TC (International Survey of Acute Coronary Syndromes in Transitional Countries) registry (NCT01218776) from October 2010 through April 2016. Patients were stratified into time-delay cohorts. We estimated the 30-day risk of all-cause mortality in each cohort. Despite …

Prolonged Growth Hormone/Insulin/Insulin-Like Growth Factor Nutrient Response Signaling Pathway As A Silent Killer Of Stem Cells And A Culprit In Aging, Mariusz Ratajczak, Andrzej Bartke, Zbigniew Darzynkiewicz

NYMC Faculty Publications

The dream of slowing down the aging process has always inspired mankind. Since stem cells are responsible for tissue and organ rejuvenation, it is logical that we should search for encoded mechanisms affecting life span in these cells. However, in adult life the hierarchy within the stem cell compartment is still not very well defined, and evidence has accumulated that adult tissues contain rare stem cells that possess a broad trans-germ layer differentiation potential. These most-primitive stem cells-those endowed with pluripotent or multipotent differentiation ability and that give rise to other cells more restricted in differentiation, known as tissue-committed stem …

Metabolic Differentiation Of Early Lyme Disease From Southern Tick-Associated Rash Illness (Stari), C Molins, L Ashton, Gary Wormser, B Andre, A Hess, J Belisle

NYMC Faculty Publications

Lyme disease, the most commonly reported vector-borne disease in the United States, results from infection with Borrelia burgdorferi. Early clinical diagnosis of this disease is largely based on the presence of an erythematous skin lesion for individuals in high-risk regions. This, however, can be confused with other illnesses including southern tick-associated rash illness (STARI), an illness that lacks a defined etiological agent or laboratory diagnostic test, and is coprevalent with Lyme disease in portions of the eastern United States. By applying an unbiased metabolomics approach with sera retrospectively obtained from well-characterized patients, we defined biochemical and diagnostic differences between early …

Outcomes Of An Integrated Approach To Speed And Strength Training With An Elite-Level Sprinter, Eric D. Magrum

Electronic Theses and Dissertations

The purpose of this study was to observe changes in sprint velocity, ground contact time, and peak force demonstrated by a competitive sprinter following an integrated approach to speed development and strength training. As part of an ongoing monitoring procedure the participant completed 20m sprint testing through an optical measurement system and isometric-strength testing before and after each phase of training. Sprint velocity, ground contact time and peak force were analysed using Tau-U, smallest worthwhile and percent change statistics. Results indicate sprinting velocity statistically improved while changes in peak force were practically significant and ground contact time remained trivial throughout …

Vulnerability Assessment Of Groundwater To No3 Contamination Using Gis, Drastic Model And Geostatistical Analysis, Adela Beauty Adu Agyemang

Electronic Theses and Dissertations

The study employed Geographical Information System (GIS) technology to investigate the vulnerability of groundwater to NO₃ content in Buncombe County, North Carolina in two different approaches. In the first study, the spatial distribution of NO₃ contamination was analyzed in a GIS environment using Kriging Interpolation. Cokriging interpolation was used to establish how NO₃ relates to land cover types and depth to water table of wells in the county. The second study used DRASTIC model to assess the vulnerability of groundwater in Buncombe County to NO₃ contamination. To get an accurate vulnerability index, the DRASTIC parameters were …

Matter Under Mind, John F. Lause

Electronic Theses and Dissertations

The artist discusses the work for his Masters of Fine Arts exhibition, Mind under Matter, held at the Tipton Gallery in downtown Johnson City, Tennessee. Exhibition dates are from March 27th through April 5th 2017. ‘Matter under Mind’ explores the balance of control and non-control within the art-making process. This technique creates an automatic dialogue resulting in abstraction guided by the subconscious. The title ‘Matter under Mind’ is a slight play on the phrase ‘mind over matter’ emphasizing how matter/material is manipulated by the mind through the making of artwork, and within the mind’s eye or imagination.

The video installation …

Effects Of Lactobacillus Rhamnosus Mrs6an On Intestinal Gpat3 Pathway Involved In The Lipid Transport In Enterocytes, Ayodeji Oludare Ipinmoroti

Electronic Theses and Dissertations

Accumulation of triglyceride (TG) in enterocytes of the small intestine follows two specific absorption pathways: the monoacylglycerol acyltransferase pathway (MGAT) and glycerol acyltransferase pathway (GPAT), the latter (GPAT) is usually found in the small intestine. In this study, we investigated the effects of Lactobacillus rhamnosus MRS6AN, an isolate from “amabere amaruranu” a Kenyan traditional cultured milk, on triglyceride accumulation and expression of GPAT3, I-FABP, MTP and NPC1L1 in Caco-2 cell enterocyte model. Intracellular triglyceride level (TG) of Caco2 cells was significantly reduced by live L. rhamnosus (LB) compared with other bacterial products. MTP expression in Caco2 cells was minimally reduced …

A Study Of Salmonid Growth In Two Southern Appalachian Headwater Streams, Joshua Argo

Electronic Theses and Dissertations

This study sampled salmonid populations in two headwater streams in East Tennessee, Briar Creek and Left Prong Hampton Creek. Length and weight data were used to calculate the growth of these populations to determine if significant variation exists between isolated brook trout populations. Slope comparisons concluded that there was a difference in growth between brook trout populations of these streams (p

Design Co2 Molecule Gas Sensor Based On Surface Plasmon Resonance, Muhamad Abdulla Hamad

Polytechnic Journal

Environmentally damaging gases, such as carbon dioxide (CO2), must be sensed for ecological observing or in the vehicle industry. CO2 gas sensing can be done theoretically through designing a biosensor based on a surface plasmon resonance (SPR) principle. The effect of gas respond can be demonstrated by means of SPR spectra shifting and a change in the utilized light reflectance. In general, The CO2 gas sensor monitors the changes of the air refractive index through measuring the position of the resonance dip value with respect to its position for normal air structure . In addition, a tiny variation in the …

Comparative Phytochemical Screening And Antioxidant Activities Of Calligonum Comosum And Calligonum Crjnitum, Khalfan Mohamed Abdulla Ai Naqbi

Theses

Medicinal plants are vital source of present day prescription and the majority of the recommended modern medications contain their subsidiaries. Calligonum comosum and Calligonum crinitum are perennial shrub plants growing in the United Arab Emirates, and are being utilized as a part of traditional medicinal system of UAE. The aims of proposed study were to evaluate and compare the phytochemical and antioxidant properties of these two plants. The extraction and fractionation were conducted by using petroleum ether, chloroform, ethyl acetate, acetone and methanol. The extracts were tested for their antioxidant activity. Phytochemical studies with total phenolics and flavonoid contents were …