Digital Commons Network^™

Retinal Progenitor Cell, Induced Pluripotent Stem Cell, And 3d Mini Retina Derived Extracellular Vesicles Contain Transcription Factors, Microrna And Protein Associated With Potency And Development, Jing Zhou

Dissertations, Theses, and Capstone Projects

Extracellular vesicles (EVs) have emerged as a novel cell-to-cell communication mechanism in recent years. EVs are membrane-covered cell fragments released into the extracellular environment by all cell types in the normal physiological and pathological conditions. These membranous extracellular organelles include exosomes (30-100 nm) and microparticles (100-1000 nm), which are believed to play a pivotal role in intercellular communication. EVs represent the way for intercellular transfer of proteins and RNAs. By transfer of genetic materials, EVs are involved in reprogramming and tissue repair. We predict that during retinal development, retinal progenitor cells release EVs containing temporally expressed mRNA, proteins and miRNA, …

The End Of Aids: Gender, Race And Class Politics In New York's Campaign To End The Epidemic, Lauren Suchman

Dissertations, Theses, and Capstone Projects

Since its official discovery in 1981, the story of HIV/AIDS has been a story of inequality. Not only has the virus spread more easily among those marginalized due to their gender, race, or class, but AIDS activism itself has tended to elevate the voices and needs of the more powerful over those with less privilege. While we might point to 1981, when the CDC issued its first official report on HIV, as the official “beginning” of HIV/AIDS, where and how does the story end? This dissertation examines one attempt to bring the story to a close: New York State’s “Ending …

Psychoanalysis And Modernity: A Failure To Find Relief From Existential Terror, Erin Liat Claridge

Dissertations, Theses, and Capstone Projects

This project considers the ways in which culture—the symbols and institutions that guide behavior and provide meaning to the individuals living within a community—serves a defensive function against conscious awareness of existential terror. The term existential terror refers to the cognitive and emotional experience of recognizing the inevitability of death, which is often accompanied by feelings of angst, isolation from others, and awareness of meaninglessness. In this dissertation, I will argue that the broad shift from traditional culture based on Judeo-Christian religious beliefs and communal forms of social life to modernity represents a slow but destabilizing deterioration of the defensive …

Bodies Of Knowledge: An Anatomy And Kinesiology Of The American Prison Nation, 'Human'-Making, And Twenty-First-Century Techno-Gods, Lyndsey Karr

Dissertations, Theses, and Capstone Projects

The social production of hegemonic knowledge has historically been legitimized in relation to the sanctioned status of the ‘Human’.[1] Beginning with the American Prison Industrial Complex and what sociologist Beth E. Richie conceptualizes as the “prison nation,” I will show the ‘human’ as a contingent and composite status appearing along a spectrum of Flesh, Body, and ‘Human’ (Flesh-Body-‘Human’) statuses and subjectivity.

Bringing this ‘Human’ continuum into conversation with twenty-first-century media, (micro)computational technologies, and contemporary knowledge and social economies, I expand the notion, reach, and scale of the American “prison nation.” Following Mark Hansen’s treatment of twenty-first-century digital media, I …

A Playful Context Enhances Bilingual And Monolingual Preschoolers’ Mastery Motivation And Private Speech, Jeremy Sawyer

Dissertations, Theses, and Capstone Projects

Children’s private speech (audible self-talk) has been studied primarily as a cognitive tool for thinking, planning and self-regulation. This study investigated whether private speech may also function as a tool for motivation. Vygotskian and self-determination theory suggest that children can develop to become agentic and inspired, or conversely disengaged and alienated, based largely on their social conditions of development. Thus, it is important to investigate children’s motivational processes in social and educational contexts that are central to child development. U.S. preschool enrollment is expanding, accompanied by a decline in play-based pedagogy and growth of didactic, teacher-centered approaches. To illuminate the …

Understanding The Role Of The Ventrolateral Prefrontal Cortex In Emotional Memory Using Transcranial Direct Current Stimulation And Transcranial Magnetic Stimulation, R. Rachel Weintraub-Brevda

Dissertations, Theses, and Capstone Projects

Emotional stimuli can have both beneficial and detrimental effects on memory, such that emotional stimuli can be distracting from current neutral working memory goals, while also leading to enhanced episodic memory for the distracting emotional stimuli. Recent evidence suggests that the ventrolateral prefrontal cortex (VLPFC) has multiple roles in the enhancing effects of emotion on memory through top-down/controlled processes, including 1) coping with negative distraction and 2) elaborative encoding of negative information. Additionally, previous research has alluded to hemispheric differences in the VLPFC (Chapter 1). However, previous research has been correlational, with no strong laterality tests of the VLPFC. Two …

Roles Of Gabab, Muscarinic And Nicotinic Receptor Signaling In The Acquisition And Expression Of Fructose And Fat-Conditioned Flavor Preferences And Acquisition Of Quinine-Conditioned Flavor Avoidances In Rats, Francis M. Rotella

Dissertations, Theses, and Capstone Projects

In addition to increased intake of sweet solutions by mammals, learning, particularly classically-conditioned “Pavlovian-like” learning, also plays an important role. An orosensory conditioned flavor preference (CFP) can be elicited by pairing one novel flavor (conditioned stimulus, CS+) with a fructose solution and a second novel flavor (CS-) with a saccharin solution. Rats will prefer the CS+ flavor in a subsequent 2-bottle choice test with both flavors mixed in saccharin. Previous pharmacological analyses revealed that systemic administration of dopamine (DA) D1 and D2 as well as NMDA, but not opioid, receptor antagonists eliminated the acquisition (learning) of fructose-CFP. Further, expression of …

Insight Into The Interaction Between The Peroxisome Proliferator-Activated Receptor Gamma (Pparγ) And Adipocyte Fatty Acid-Binding Protein (A-Fabp), Qian Wang

Dissertations, Theses, and Capstone Projects

The Adipocyte Fatty Acid-Binding Protein (AFABP) is mainly expressed in fat cells. It can bind fatty acids and other lipophilic substances such as eicosanoids and retinoids. The peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor protein that requires ligand binding to regulate the specific gene transcription. PPARγ is expressed at extremely high levels in adipose tissue, macrophages, and the large intestine, where it controls lipid adipogenesis and energy conversion. Moreover, it has been found that AFABP and PPARγ can form a complex in vivo. It is proposed that AFABP carries the ligand and enters into the nucleus where it …

Parallels Of Love And Hate Defined By Attachments, Nicole Spann

Dissertations, Theses, and Capstone Projects

The assertion, “It’s a thin line between love and hate,” is often referenced to express the relative ease of a sole emotion morphing from one extreme to next. Both love and hate generate the same physiological fluctuations which include, but are not limited to, a racing pulse, sweaty palms, releasing of hormones, and some degree of excitability. While physiologically the responses are the same, psychologically and philosophically they are considered opposites. In the sphere of psychology, it is going from what is considered normal behavior or a normal response to abnormal behavior or an abnormal response (McKay, 2016). For this …

Morphogenesis And Growth Driven By Selection Of Dynamical Properties, Yuri Cantor

Dissertations, Theses, and Capstone Projects

Organisms are understood to be complex adaptive systems that evolved to thrive in hostile environments. Though widely studied, the phenomena of organism development and growth, and their relationship to organism dynamics is not well understood. Indeed, the large number of components, their interconnectivity, and complex system interactions all obscure our ability to see, describe, and understand the functioning of biological organisms.

Here we take a synthetic and computational approach to the problem, abstracting the organism as a cellular automaton. Such systems are discrete digital models of real-world environments, making them more accessible and easier to study then their physical world …

A Combined Computational Strategy Of Sequence And Structural Analysis Predicts The Existence Of A Functional Eicosanoid Pathway In Drosophila Melanogaster, Michael Scarpati

Dissertations, Theses, and Capstone Projects

With increased understanding of their roles in signal transduction and metabolism, eicosanoids have emerged as important players in human health and disease. Mammalian prostanoids and related lipid mediators perform varied functions in different tissues and organs. Synthesized through the oxygenation of C20 polyunsaturated fatty acids, mammalian eicosanoids are both pro- and anti-inflammatory. The physiological contexts in which eicosanoid family members act at the cellular level are not well understood. In this study, we examined whether the genome of Drosophila melanogaster, a powerful model for innate immunity and inflammation, codes for the enzymes required for eicosanoid biosynthesis. We report the …

Grounding, Causation And The Unity Of Ontological Structure, Thomas Kivatinos

Dissertations, Theses, and Capstone Projects

Unity is enchanting. That is, it is an enchanting idea that although reality seems to be fragmented in various ways, this apparent fragmentation belies a fundamental unity. This dissertation is an attempt at theoretically capturing such unity via the theoretical unification of causation and grounding. And this unification supports another: the unification of reality’s causal structure and reality’s hierarchical structure.

To propose a unification of causation and grounding, I employ a common conception of grounding on which grounding is understood as a dependence relation. On this conception, if the Xs ground the Ys, then the existence of …

Modulation Of Spasticity By Trans-Spinal Direct Current Stimulation In Animals With Spinal Cord Injury, Wagdy Mekhael

Dissertations, Theses, and Capstone Projects

Central nervous system injuries usually produce motor impairments that are exacerbated by pathologically altered muscle tone. Abnormal muscle tone interferes with voluntary movement and is associated with loss of dexterity. Prior work in our laboratory demonstrated that 30-second trans-spinal direct current (DC) stimulation can temporarily modify muscle tone in anesthetized spastic mice after spinal cord injury (SCI). These experiments described DC-induced muscle tone responses to be polarity-dependent. That is, anodal stimulation (current passed from the lumbar spine to sciatic nerve) decreased muscle tone, and cathodal stimulation (current passed from the sciatic nerve to the lumbar spine) increased it. The present …

Capture And Abandon: Social Reproduction And Informal Land Tenure In Jamaica, Rachel Goffe

Dissertations, Theses, and Capstone Projects

Capture and Abandon: Social Reproduction and Informal Land Tenure in Jamaica examines how ongoing policy development to curtail squatting is shifting state capacity away from a project of land reform and towards one of land management. Scholarship about informal settlements elsewhere tends to understand dispossession as a project of the neoliberal state. I argue that it is strategically necessary to elucidate the ways in which the production of durable but insecure access to land is not novel, but is imbricated in the historical production of a Black labor force that is self-sufficient and yet ready at hand, reproducing themselves through …

Genre, Representation, And Memory In Spanish Civil War Texts By Women From Spain And The United States, Jennifer Prince

Dissertations, Theses, and Capstone Projects

This dissertation seeks to addresses a lacuna in the androcentric Spanish Civil War literary canon by recovering women’s voices writing about the war from the 1930s to the present. It also examines the war stories women tell and how they represent themselves and others when writing about the Spanish Civil War. All of the seven authors examined here write through the lens of some distance—either as American citizens observing the war or as the descendants of the war’s survivors—but each with an intimate connection rooted in biology or ideology. The foundation of this dissertation is close reading and textual analysis …

Past Peer Victimization Experiences And Current Psychological Well-Being And Ethnic Identity Among South Asian College Students, Rejitha Nair

Dissertations, Theses, and Capstone Projects

Very little is known about the peer victimization experiences of South Asian immigrant students and the factors involved in these experiences. The present study retrospectively investigated the peer victimization experiences of South Asian immigrant students in high school, the perceived reasons for victimization, and how these experiences relate to their psychological well-being and ethnic identity as college students. Two hundred and twenty college students, who were first or second generation immigrants from South Asia (e.g., India, Pakistan, Afghanistan, Bangladesh, Sri Lanka, and Nepal) and attended high school in the United States, participated in the study.

Overall, the results revealed that …

Mechanisms Of Navigation In Fiddler Crabs: An Analysis Of Allocentric And Egocentric Contributions, Stephen G. Volz

Dissertations, Theses, and Capstone Projects

Navigation in biological systems is a complex task-set that involves learning processes and may include constructing representations of features of their environment. Across the animal kingdom, different learning mechanisms have evolved to similar spatial problems. The extent to which mechanisms are conserved across taxa are an important research area that can guide our understanding of the cognitive dimensions of navigation. Recent studies of mammals, birds, and arthropods has found that these animals often attend to multiple forms of sensory cues, and to either integrate the solutions generated by these cues, or at times prefer one form of cue over another. …

The Role Of Nucleolin Phosphorylation By Ck2 In Regulating Cellular Fate Under Normal And Stress Conditions, Shu Xiao

Dissertations, Theses, and Capstone Projects

Nucleolin (NCL or C23) is an abundant genotoxic stress-responsive RNA binding phosphoprotein. NCL constitutes 10% of total nucleolar protein that has functions in multiple biological processes, including ribosome biogenesis, DNA/RNA metabolism, cellular response to DNA damage, cell growth, proliferation and death. In this dissertation, I elucidate the role of nucleolin phosphorylation by casein kinase 2 (CK2) in controlling cellular fate by regulating p53 checkpoint under normal and stressed conditions. First, I demonstrate that the six consensus CK2 sites on the N-terminus of NCL are important for cell survival and proliferation. Expression of CK2 phosphorylation-deficient NCL mutant leads to dominant negative …

Climate-Driven Habitat Shifts In South America: Biogeography, Historical Demography, And Population Genomics Of Anole Lizards, Ivan Prates

Dissertations, Theses, and Capstone Projects

Shifts in the geographic distribution of habitats over time can promote dispersal and vicariance, thereby influencing large-scale biogeographic patterns and ecological processes. The establishment of corridors of suitable habitat across previously disjunct yet ecologically similar regions has been widely linked to climate change over time. Such climate-mediated habitat changes may have played a key role in the assembly of tropical biotas, including those of Amazonia and the coastal Atlantic Forest in South America, two of the most diverse ecosystems on Earth. The history of biogeographic associations between tropical rainforest habitats in response to former climatic shifts, as well as their …

The Role Of T-Box Proteins In Vertebrate Germ Layer Formation And Patterning, Sushma Teegala

Dissertations, Theses, and Capstone Projects

All of the tissues in triploblastic organisms, with the exception of the germ cells, arise from the three germ layers, ectoderm, mesoderm and the endoderm. The identification of the genes that underlie the differentiation of these layers is crucial to our understanding of development. T-box family proteins are DNA-binding transcriptional regulators that play important roles during germ layer formation in the early vertebrate embryo. Well-characterized members of this family, including the transcriptional activators Brachyury and VegT, are essential for the proper formation of mesoderm and endoderm, respectively. To date, T-box proteins have not been shown to play a role in …

Trypanosome Lytic Factor Mediated Immunity Against Leishmania Sp., Jyoti Pant

Dissertations, Theses, and Capstone Projects

Trypanosome Lytic Factor (TLF) is an innate immunity complex that was originally discovered to protect against African Trypanosomes. The major components of TLF are Apolipoprotein A1 (APOA1), Apolipoprotein L1 (APOL1) and HPR (Haptoglobin Related Protein), where APOL1 is necessary and sufficient for trypanolysis. Recently we have shown that TLF ameliorates infections by cutaneous Leishmania species. Here we investigated the effect of different primate and human TLF against different Leishmania sp. Our result shows that TLF kills metacyclic promastigotes of cutaneous Leishmania sp. within immune cells such as neutrophils and macrophages by two different mechanism. Using transiently transfected and germline transgenic …

Natural History Collections: Teaching About Biodiversity Across Time, Space, And Digital Platforms, Anna K. Monfils, Karen E. Powers, Christopher J. Marshall, Christopher T. Martine, James F. Smith, L. Alan Prather

Biology Faculty Publications and Presentations

Natural history collections offer unique physical and virtual opportunities for formal and informal progressive learning. Collections are unique data in that they each represent a biological record at a single place and time that cannot be obtained by any other method. Collections-based experiences lead to an increased understanding of and substantive interaction with the living world. Global biological diversity and changes in that diversity are directly tracked through specimens in collections, regardless of whether changes are ancient or recent. We discuss how collections, specimens, and the data associated with them, can be critical components linking nature and scientific inquiry. Specimens …

Α-Conotoxin Decontamination Protocol Evaluation: What Works And What Doesn’T, Matthew W. Turner, John R. Cort, Owen M. Mcdougal

Chemistry and Biochemistry Faculty Publications and Presentations

Nine publically available biosafety protocols for safely handling conotoxin peptides were tested to evaluate their decontamination efficacy. Circular dichroism (CD) spectroscopy and mass spectrometry (MS) were used to assess the effect of each chemical treatment on the secondary and primary structure of α-CTx MII (L10V, E11A). Of the nine decontamination methods tested, treatment with 1% (m/v) solution of the enzymatic detergent Contrex™ EZ resulted in a 76.8% decrease in α-helical content as assessed by the mean residue ellipticity at 222 nm, and partial peptide digestion was demonstrated using high performance liquid chromatography mass spectrometry (HPLC-MS). Additionally, …

The Pedagogical Influences Of A Value-Added Model Evaluation System From The Perspectives Of Elementary School Teachers In North Georgia: A Phenomenological Study, Kyle Shugart

Doctoral Dissertations and Projects

The purpose of this phenomenological study was to describe the pedagogical influences of the value-added model of evaluation as experienced by elementary school teachers in a North Georgia suburban school district. A transcendental phenomenological design was used to provide a voice to (N = 12) elementary school teachers evaluated with a value-added model evaluation system through the lens of Bronfenbrenner’s (1976) social ecological model of the educational environment and Bandura’s (1977) social cognitive theory as it related to mastery experiences of the teacher. Data collection methods included interviews, a focus group, and evaluation documentation. The data were analyzed according to …

A Marker Of Biological Age Explains Individual Variation In The Strength Of The Adult Stress Response, C Andrews, D Nettle, M Larriva, R Gillespie, Et Al

School of Biological and Marine Sciences

The acute stress response functions to prioritize behavioural and physiological processes that maximize survival in the face of immediate threat. There is variation between individuals in the strength of the adult stress response that is of interest in both evolutionary biology and medicine. Age is an established source of this variation—stress responsiveness diminishes with increasing age in a range of species—but unexplained variation remains. Since individuals of the same chronological age may differ markedly in their pace of biological ageing, we asked whether biological age—measured here via erythrocyte telomere length—predicts variation in stress responsiveness in adult animals of the same …

Autophagy In Mycobacterium Abscessus Infection, Bindu Subhadra, Chul Hee Choi

Faculty Publications - Biological Sciences

Autophagy is a self-degradative process that removes misfolded or aggregated proteins, clears damaged organelles, as well as eliminates intracellular pathogens playing a role in innate immunity. Mycobacterium abscessus (M. abscessus) has been reported as a causative organism in nearly 80% of the rapid growing mycobacteria (RGM) pulmonary disease. The strain exhibits two different colony types: the smooth (S) one which is considered wild-type and the rough (R) one which is the mutated strain. In accordance to the colony morphology, the S and R types display varying autophagic responses in the host cells with the R type inducing elevated autophagy compared …

Impact Of Introduced Parasitoid Cotesia Vestalis (Hymenoptera: Braconidae) On Plutella Xylostella (Lepidoptera: Plutellidae) And Its Parasitoid Guild On Kale In Semi-Arid Areas In Kenya, Ruth Kahuthia-Gathu, Brigitte Nyambo, Sevgan Subramanian

All Peer-Reviewed Publications

Cotesia vestalis was introduced into Kenya from South Africa in March 2003 for biological control of Plutella xylostella with initial release in 2004. To confirm establishment, the rate of spread beyond the release sites and parasitism rates by C. vestalis were assessed between October 2011 and April 2012 in eastern and southeastern Kenya (Kitui, Mwingi/Yatta and Loitokitok areas). Monthly observations on kale infestation by diamondback moth, damage scores, parasitism and parasitoid guild were undertaken on 20 randomly selected plants in farmer-managed kale farms. Sampled larval and pupal stages were placed in containers and taken to the laboratory for parasitoid emergence. …

The History And Rationale Of Swedish Prostitution Policies, Sven-Axel Månsson

Dignity: A Journal of Analysis of Exploitation and Violence

This article analyses the history and rationale behind “the Swedish model” of regulating prostitution. The most controversial and debated part of this model is the 1999 ban on purchases of sexual services. To be fully understood the ban and the comprehensive policy regime of which it is a part, the new model has to be placed within a broader framework of policy areas such as gender, sexuality, and social welfare. Thus, the contemporary policy regime will be traced back to the mid-1970s when gender norms and sexual mores were renegotiated in Sweden, which in turn led to a radical reconsideration …

Summaries Of Arkansas Cotton Research 2016, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Discovering Forensic Fraud, Jennifer D. Oliva, Valena E. Beety

Northwestern University Law Review

This Essay posits that certain structural dynamics, which dominate criminal proceedings, significantly contribute to the admissibility of faulty forensic science in criminal trials. The authors believe that these dynamics are more insidious than questionable individual prosecutorial or judicial behavior in this context. Not only are judges likely to be former prosecutors, prosecutors are “repeat players” in criminal litigation and, as such, routinely support reduced pretrial protections for defendants. Therefore, we argue that the significant discrepancies between the civil and criminal pretrial discovery and disclosure rules warrant additional scrutiny.

In the criminal system, the near absence of any pretrial discovery means …