Digital Commons Network^™

Mechanism Of Gene Regulation By Coding Polya Tracks, Laura Lea Arthur

Arts & Sciences Graduate Student Theses and Dissertations

Regulation of gene expression is essential for cellular development and survival. The great variety and complexity of regulatory mechanisms underscores this fact. Messenger RNA stability and translational efficiency are often key determinants of gene expression. mRNA surveillance pathways, discovered for their role in degradation of aberrant mRNA, are now known to be instrumental in the regulation of physiologically correct mRNA stability. Thus, the study of cis elements in a transcript that can induce mRNA surveillance pathways has become an area of particular interest.Here I report on the mechanism of gene regulation by coding polyA tracks, defined as a sequence of …

Understanding The Paradox Of Genetic Diversity In Uropathogenic E. Coli: The Uncommon Evolution Of A Common Pathogen, Henry Louis Schreiber

Arts & Sciences Graduate Student Theses and Dissertations

Urinary tract infections (UTIs) are the second most common bacterial infection of people in the U.S.A and are frequently recurrent, as an initial UTI is quickly followed by a second episode in 30-35% of cases despite appropriate antibiotic treatment and clearance of the bacteria from the urine. The vast majority, >80%, of UTIs are caused by uropathogenic Escherichia coli (UPEC). UPEC that colonize the bladder are thought to originate in the gut, where they live as commensal organisms. UPEC can be shed in feces to colonize the vagina and/or periurethral area, and then can ascend into the bladder to start …

Investigation Of Neonatal Pulmonary Structure And Function Via Proton And Hyperpolarized Gas Magnetic Resonance Imaging, Nara Savoye Higano

Arts & Sciences Graduate Student Theses and Dissertations

Magnetic resonance imaging (MRI) is a modality that utilizes the phenomenon of nuclear magnetic resonance (NMR) to yield tomographic images of the body. Proton (1H) MRI has historically been successful in soft tissues but has suffered in the lung due to a variety of technical challenges, such as the low proton-density, rapid T2* relaxation time of the lung parenchymal tissue, and inherent physiological motion in the chest. Recent developments in radial ultrashort echo time (UTE) MRI have in part overcome these issues. In addition, there has been much progress in techniques for hyperpolarization of noble gases (3He and 129Xe) out …

Bridging The Translational Gap Between Rodent And Human Pain Research, Tayler Diane Sheahan

Arts & Sciences Graduate Student Theses and Dissertations

The treatment of chronic pain is an immense clinical and societal burden rooted in the ineffectiveness and adverse side effects of existing analgesics. Extensive efforts have been directed towards the development of novel pain therapies with maximal efficacy and minimal unwanted effects; however, putative therapeutic targets identified in preclinical rodent models rarely translate in clinical trials. The poor translational record of basic pain research findings has been attributed, in part, to the use of suboptimal rodent pain models and behavioral endpoints used to assess putative analgesics, as well as differences in the pharmacological profiles of rodents and humans. The work …

The Chemistry And Biology Of Iron-Scavenging Natural Products From Pathogenic Acinetobacter Baumannii, Justin Shapiro

Arts & Sciences Graduate Student Theses and Dissertations

Multidrug resistant (MDR) Gram-negative bacteria represent a global health crisis. Traditional antibiotics create selective pressure and breed resistance, making our current drugs less and less effective. Targeting of bacterial virulence factors has gained interest as an alternative strategy to potentially circumvent this problem. Virulent Acinetobacter baumannii synthesize and secrete pre-acinetobactin, a small molecule metal chelator that scavenges life-sustaining iron and is critical for establishing infection. The work described herein sheds light on the unusual properties of a non-enzymatic isomerization from pre-acinetobactin to acinetobactin and proposes a siderophore-swapping mechanism by which A. baumannii expands its pH window for virulence. Knowledge from …

Bio-Inspired Multi-Spectral And Polarization Imaging Sensors For Image-Guided Surgery, Nimrod Missael Garcia

McKelvey School of Engineering Graduate Student Theses & Dissertations

Image-guided surgery (IGS) can enhance cancer treatment by decreasing, and ideally eliminating, positive tumor margins and iatrogenic damage to healthy tissue. Current state-of-the-art near-infrared fluorescence imaging systems are bulky, costly, lack sensitivity under surgical illumination, and lack co-registration accuracy between multimodal images. As a result, an overwhelming majority of physicians still rely on their unaided eyes and palpation as the primary sensing modalities to distinguish cancerous from healthy tissue. In my thesis, I have addressed these challenges in IGC by mimicking the visual systems of several animals to construct low power, compact and highly sensitive multi-spectral and color-polarization sensors. I …

Comparative Molecular Characterization Of Typical And Exceptional Responders In Glioblastoma, Kristin Wipfler

Theses & Dissertations

Glioblastoma (GBM) is the most common and the deadliest type of primary brain tumor, with a median survival time of only 15 months despite aggressive treatment. Although most patients have an extremely poor prognosis, a small number of patients survive far beyond the median survival time. Investigation of these “exceptional responders” has sparked a great deal of interest and is becoming an important focus in the field of cancer research. To investigate the molecular differences between typical and exceptional responders in GBM, comparative analyses of copy number, methylation, gene expression, miRNA expression, and protein expression data sets from The Cancer …

Evaluation Of Aminopyrazole Analogs As Cyclin-Dependent Kinase Inhibitors For Colorectal Cancer Therapy, Caroline Robb

Theses & Dissertations

Colorectal cancer (CRC) remains one of the leading causes of cancer related deaths in the United States. Currently, there are limited therapeutic options for CRC patients, none of which focus on the cell signaling mechanisms controlled by members of the cyclin dependent kinase (CDK) family. CDK5 has been implicated in a variety of cancers, and most recently as a tumor promoter in CRC.

As such, we evaluated a compound developed by Pfizer, CP-668863 (a.k.a. 20-223), that inhibits CDK5 in neurodegenerative disorders. In our CRC xenograft model, 20-223 reduced tumor growth and tumor weight, indicating its value as a potential anti-CRC …

Organizational Effects Of Defeminizing Toxicants: Lessons Learned From An Environmental Sentinel Organism, The Fathead Minnow., Jonathan Ali

Theses & Dissertations

Endocrine disrupting compounds (EDCs) are chemicals that interfere with hormone function and are increasingly detected in aquatic environments, where they elicit adverse effects from exposed organisms. The toxicological effects of EDCs can be described as either activational (reversible) or organizational (irreversible), where the latter are associated with adverse outcomes in reproductive performance of adult fish. However, few studies have investigated the organizational impacts of anti-estrogenic or “defeminizing” EDCs, e.g. agrichemicals or pharmacological agents, in an environmentally-relevant or “sentinel” species. The objective of this study was to investigate the impacts of early-life EDC-initiated changes in estrogenic gene expression on organizational effects …

Relationships Between Complex Pediatric Health Conditions And The Built, Physical And Social Environments, Brittany Corley

Theses & Dissertations

The relationship between human health and the environment is complex and overall poorly understood. The environment has a lasting impact on health, and the processes appear to start at conception. Thus, this dissertation examines the potential relationship between pediatric health and the environment.

The first relationship examined was between the built environment and comorbid Attention Deficient Hyperactivity Disorder and Autism Spectrum Disorder. Many publications have reported the relationship between socioeconomic disadvantage, Attention Deficient Hyperactivity Disorder, and Autism Spectrum Disorder. However, the link between the built environment and Attention Deficient Hyperactivity Disorder and Autism Spectrum Disorder symptom severity is poorly understood. …

Identifying The Role Of Janus Kinase 1 In Mammary Gland Development And Breast Cancer, Barbara Swenson

Theses & Dissertations

The development of the postnatal mammary gland is tightly controlled by peptide hormones and cytokines. The signaling of these extracellular ligands through their corresponding receptors rely on Janus Kinases (JAKs) that activate downstream Signal Transducers and Activators of Transcription (STATs). The JAK/STAT signaling pathway is crucial for processes such as growth, proliferation, and cell survival of the epithelial tissue, but also for the breakdown and remodeling of the mammary gland via IL-6 class inflammatory cytokines (e.g. LIF and OSM). JAK1 and JAK2, which are expressed in the mammary gland, are thought to have redundant functions. However, our previous studies demonstrated …

Perturbing Anti-Apoptotic Proteins To Develop Novel Cancer Therapies, Jacob Contreras

Theses & Dissertations

The apoptotic pathway involves a tightly regulated network of proteins which respond to various stimuli. Previous studies have indicated Mcl-1 and Bcl-xL are intimately involved in determining cell fate, and if both are concurrently neutralized, it activates the apoptotic pathway. The inactivation of Bcl-xL and Mcl-1 as a mechanism to trigger the intrinsic apoptotic response can be used as a platform to develop therapeutic strategies to target cancer cells. The apoptotic pathway is largely dysregulated and often leads to therapy resistance in cancer cells. Although direct inhibitors of Bcl-xL have been developed and have advanced to clinical trials, development of …

Maternal Life Course Adversity: The Intersection Of Psychosocial And Biobehavioral Adaptive Response In Pregnancy, Crystal Modde Epstein

Theses & Dissertations

The link between life course adversity and adverse health outcomes is well established, particularly early life adversity (ELA). There is also evidence that the physiologic adaptations associated with stress, depression and ELA can be transmitted intergenerationally via long-term set-point changes within the maternal hypothalamic-pituitary-adrenal axis (HPA). It is unknown how the type and timing of maternal stress and adversity influences HPA regulation during pregnancy and whether maternal coping attenuates this relationship. Manuscript 1 was an integrative review of studies examining the association between maternal ELA and HPA regulation during pregnancy. In manuscripts 2 and 3 the findings of the dissertation …

Life Being An International Student In The United States: Acculturation, Culture Shock, And Identity Transformation, Lai Yan Vivyan Lam

Master's Theses

The population of international students at community colleges in the United States has increased significantly over the past decade. International students play a big role in building the cultural diversity on campus by bringing over different cultures and sharing their global perspective to the local community. However, they often face challenges adapting into American culture due to cultural differences in education system, language, lifestyle, etc. By looking into the acculturation process of international students to analyze the culture shock and cultural identity changes they experienced, this paper intends to seek ways to help this group of students to ease their …

Exploring Employment Equity: Diversity & Inclusion In Canada’S Legislation & Policy, Vanessa Toussaint Miller

Master's Theses

Multiculturalism is considered very much a part of Canada’s national heritage and identity, becoming the first nation to adopt multiculturalism as an official policy in 1971. Over the last 40 years, the Canadian population has undergone significant changes bringing in immigrants with different cultures, ideologies, religions and ways of life. Despite the various changes in society, the multiculturalism policies have remained the same. This thesis examines Canada’s Employment Equity-a policy intended to eliminate systemic discrimination in the workplace-and its effectivity at creating a diverse and inclusive workforce. The main research question is, in the past five years, has Employment Equity …

Assessing The Presence Of Marine Toxins In Bivalve Molluscs From Southwest India, Ad Turner, Monika Dhanji-Rapkova, Stephanie Rowland-Pilgrim, Lm Turner, Ashwin Rai, Moleyur N. Venugopal, Indrani Karunasagar, Anna Godhe

School of Biological and Marine Sciences

No abstract provided.

In Vitro Toxic Effects Of Reduced Graphene Oxide Nanosheets On Lung Cancer Cells, Ta Tabish, Mzi Pranjol, H Hayat, Aam Rahat, Et Al

School of Engineering, Computing and Mathematics

The intriguing properties of reduced graphene oxide (rGO) have paved the way for a number of potential biomedical applications such as drug delivery, tissue engineering, gene delivery and bio-sensing. Over the last decade, there have been escalating concerns regarding the possible toxic effects, behaviour and fate of rGO in living systems and environments. This paper reports on integrative chemical-biological interactions of rGO with lung cancer cells, i.e. A549 and SKMES-1, to determine its potential toxicological impacts on them, as a function of its concentration. Cell viability, early and late apoptosis and necrosis were measured to determine oxidative stress potential, and …

Transgender Bathroom Rights In The Time Of Trump, Catherine Jean Archibald

Tennessee Journal of Race, Gender, & Social Justice

On May 13, 2016, the Obama Administration issued a guidance document entitled “Dear Colleague Letter on Transgender Students,” which interpreted federal law as requiring that transgender students be permitted to use bathrooms that correspond with their gender identity in schools receiving federal funding. Considerable litigation ensued. Less than a year later, on February 22, 2017, the Trump Administration rescinded this guidance document. The Trump Administration asserts that it should be up to individual states to decide what bathrooms transgender children may use in schools.

This article addresses where transgender bathroom rights stand now that Obama’s Dear Colleague Letter has been …

Title Ix's Field Of Unrealistic Dreams: If You Build It, Will They Come?, Becky L. Jacobs

Tennessee Journal of Race, Gender, & Social Justice

No abstract provided.

Contributor's Notes

Fishladder: A Student Journal of Art and Writing

No abstract provided.

Marathon, Zach Sheneman

Fishladder: A Student Journal of Art and Writing

No abstract provided.

An Impossible Ideal: Motherhood In Eighteenth-Century Britain, Jessica Hanselman Gray

Criticism

No abstract provided.

A Qualitative Examination Of The Listening Effort Experience Of Adults With Hearing Loss, Alison Marinelli

Doctoral Dissertations

Increased effort and fatigue are common complaints of individuals with hearing loss, however the listening effort (LE) experience from the patient perspective is not well understood. Current models note the importance of cognitive factors in LE. Research, that is largely quantitative in nature, has shown equivocal findings regarding auditory signals that may cause increased LE, technologies that may ameliorate the LE experience, and the effects of LE on sensory systems. Very limited work has explored the personal aspects of LE. As a whole, however, investigations of LE fail to define the experience from the patient’s view or offer suggestions for …

Exploring The Impacts That An Urban Public High School Experience Has On Bridgewater State University Students’ Perceived College Preparedness, Katherine R. Trudell

Honors Program Theses and Projects

College preparedness is a widely researched topic within education. Previous research has found that an individual’s college readiness is primarily determined by the rigor of their high school academics, their involvement in extracurricular activities, and their knowledge of the higher educational system (Conley 2008; Glater 2016; Glennie et al. 2015; Holles 2016). The purpose of this project was to investigate whether or not Bridgewater State University students felt that these factors affected their individual preparedness for college. For this project, I interviewed 18 students who attended urban public high schools in Southeastern Massachusetts in order to explore whether or not …

V. 85, Iss. 11, December 14, 2017

Archway (1946-2020)

No abstract provided.

Species Named In Honor Of Professor Emeritus Dr. Jim Braselton, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Winter Commencement: December 14-15, 2017, University Of North Dakota

UND Commencement Programs

UND Winter Commencement program from December 14 and 15, 2017.

Gardner-Webb Alumnus Recognized For Undergraduate Research, Office Of University Communications

Gardner-Webb NewsCenter Archive

The summer before Christopher Lile graduated from Gardner-Webb University, he spent five weeks studying small mammals on the Broad River Greenway and the surrounding area. The research experience helped him secure internships in Indiana and Madagascar. Recently, the Waynesville, N.C., native received another honor for his work.

Soft Foam Robot With Caterpillar-Inspired Gait Regimes For Terrestrial Locomotion, Cassandra M. Donatelli, Zachary T. Serlin, Piers Echols-Jones, Anthony E. Scibelli, Alexandra Cohen, Jeanne-Marie Musca, Shane Rozen-Levy, David Buckingham, Robert White, Barry A. Trimmer

Engineering Faculty Articles and Research

Caterpillars are the soft bodied larvae of lepidopteran insects. They have evolved to occupy an extremely diverse range of natural environments and to locomote in complex three-dimensional structures without articulated joint or hydrostatic control. These animals make excellent bio-inspiration for the field of soft robotics because of their diversity and adaptability. In this paper, we present SquMA Bot, a caterpillar-inspired soft robot. The robot's body is primarily composed of a soft viscoelastic foam, and it is actuated using a motor-tendon system. SquMA Bot is able to mimic the inching gait of a caterpillar and can use its flexible body to …

Dream And Reality: The Selective Implementation Of The Kessler Plan In Dallas, Molly A. Plummer

Landscape Architecture Masters & Design Theses - Archive

This research examines George Kessler’s 1911 A City Plan for Dallas to understand the gaps between the ideals of the City Beautiful era plan and impact on the realized physical design of the city. As inequality rises in our urban centers (insert citation), it is increasingly important for us to understand our social and political environments that shaped the landscapes that physically reinforce that inequity. As stated by Roy Rosenzweig and Elizabeth Blackmar in their history of Central Park, “its meaning as a public institution also has two dimensions: its political character as property and its cultural character as an …