Digital Commons Network^™

Living In The World, David Penberg

Occasional Paper Series

Penberg's essay highlights that through teaching abroad, he learned to respect the variety of human associations and multiple forms of intelligence. Travel has been an inextricable part of his education. It has enriched his teaching and contributed to his personal development.

Complete Volume 3, Mcnair Scholars

The Winthrop McNair Research Bulletin

No abstract provided.

Published Elsewhere And Thank You

The Winthrop McNair Research Bulletin

No abstract provided.

Investigating X Chromosome Non-Disjunction In Drosophila Melanogaster Su(Var)3-9 Mutants, Camerun C. Washington, Kathryn Kohl

The Winthrop McNair Research Bulletin

Meiotic recombination is a highly regulated process necessary for promoting proper chromosome disjunction during the first meiotic division. Notably, reduced levels of meiotic recombination are observed in heterochromatic regions of the chromosome. This study seeks to investigate the molecular mechanisms underlying this observation by examining the effects of reduced heterochromatin on non-disjunction rates in Drosophila melanogaster. To accomplish this, we measured non-disjunction in wild-type and reduced heterochromatin mutant su(var)3-9 flies. To begin, we confirmed the presence of a mutation within su(var)3-9 via Sanger sequencing. Next, we created allele-specific primers and designed a PCR protocol to more accurately identify mutant …

Targeted Mutagenesis Of Fox0 Transcripts Using The Model Organism Ciona Intestinalis Utilizing The Crispr/Cas9 System, Nicole Mcmullen, Heather J. Evans-Anderson

The Winthrop McNair Research Bulletin

The chordate, Ciona intestinalis, has become an excellent model organism for the study of cardiac development. C. intestinalis is a member of the phylum Tunicata, which is the sister phylum to vertebrates. Since both phyla share a common ancestor, an orthologous gene has been identified in C. intestinalis and vertebrates, the FOX0 transcript. Multiple reasons makes Ciona intestinalis an interesting model organism. For instance, ease of access to the animal, close relation to vertebrates, and mapping of the genetic code of Ciona intestinalis makes this an excellent model organism. The FOX0 family of transcripts plays an important role in …

Controlling Oct4 Expression Levels Using Invitrogen’S Geneswitch™ System, Autumn S. Leggins, Nick Grossoehme, Matthew M. Stern

The Winthrop McNair Research Bulletin

Oct4 is a protein that is involved in the retention of pluripotency in adipose derived stem cells (ADSCs). Despite this knowledge, Oct4’s exact role in the complex system used in maintaining pluripotency is not known. One approach to explore Oct4’s role would be through the use of cellular assays to control the expression of Oct4. This can possibly be accomplished by introducing a biological switch and the gene of interest into ADSCs. In this project, the GeneSwitch™ System is used to ultimately induce Oct4 expression. Before the GeneSwitch™ System can be used, the Oct4 gene is extracted from murine embryonic …

Evaluation Of The Potential Hmga1-Ef24 Nexus In Human Colon Cancer, Madeline Diaz, Takita Sumter

The Winthrop McNair Research Bulletin

The architectural chromatin binding proteins High Mobility Group A1 (HMGA1) are proteins expressed at high levels in malignant cancers and induce neoplastic transformation. The protein is increased as the last step of the Wnt/B-catenin/TCF-4 pathway and mediates drug resistance, therefore correlating with a poor patient prognosis. HMGA1-mediated chemoresistance results from a self-protective process called cellular senescence. Analogs of the antioxidant, curcumin, when used in combination with traditional chemotherapeutic agents, are useful treatment options for drug resistant tumors. This study had two specific aims. The first being to investigate how colon cancer cells HCT116 respond to treatment with EF24. …

Business Sustainability: An Analysis Of Three Enterprises In Charlotte, North Carolina, Cera T. Crowe, Padmini Patwardhan Dr.

The Winthrop McNair Research Bulletin

Sustainability is defined in varying ways by businesses for reasons such as industry, stakeholder expectations, compliance, and other factors. In this case study, three companies, Domtar, Duke Energy, and Nucor, are examined on their approach to reporting and practicing environmental, social, and economic sustainability. Content analysis was used to identify themes in corporate paid, owned, and earned media. Interviews with facility managers were used to understand how management implements sustainable practices on a facility level. This case study examines in detail how these companies use different language, tools, and systems to progress sustainable development in a globalized economy. Results showed …

Front Cover

The Winthrop McNair Research Bulletin

No abstract provided.

The Library’S Role In Data Management Support: A Perspective From Uc Libraries, Amy Koshoffer

Ohio IR Day

Amy Koshoffer, of the University of Cincinnati, will give the keynote presentation discussing the University of Cincinnati Libraries' perspective on the library’s role in data management support.

As A Last Resort, Ask The Students: What They Say Makes Someone An Effective Law Teacher, James B. Levy

Maine Law Review

There is an adage among doctors that “as a last resort, ask the patient.” It is a not so facetious reference to the observation that because doctors are so highly educated and trained, they can start to believe they know what’s best for their patients better than the patients themselves. Consequently, these doctors may discount, or altogether ignore, the opinions of the very people they are suppose to be helping. The same observation could be made about the law professor-student relationship. Unlike doctors, though, our relationship with students is hierarchical, and thus we may be even less inclined to “ask …

Unilateral And Multilateral Preventive Self-Defense, Stéphanie Bellier

Maine Law Review

The governing principle of the collective security system created by the United Nations Charter in 19451 is the rule prohibiting the use of force in Article 2(4), which provides that "All Members shall refrain in their international relations from the threat or use of force against the territorial integrity or political independence of any state, or in any other manner inconsistent with the Purpose of the United Nations." This rule prohibiting the use of force was considered revolutionary at the time because it transformed into international law ideas which had for centuries, if not millennia, preoccupied the minds of people …

The Role Of Argumentation In Hypothetico-Deductive Reasoning During Problem-Based Learning In Medical Education: A Conceptual Framework, Hyunjung Ju, Ikseon Choi

Interdisciplinary Journal of Problem-Based Learning

One of the important goals of problem-based learning (PBL) in medical education is to enhance medical students’ clinical reasoning—hypothetico-deductive reasoning (HDR) in particular—through small group discussions. However, few studies have focused on explicit strategies for promoting students’ HDR during group discussions in PBL. This paper proposes a novel conceptual framework that integrates Toulmin’s argumentation model (1958) into Barrows’s HDR process (1994). This framework explains the structure of argumentation (a claim, data, and a warrant) contextualized in each phase of HDR during PBL. This paper suggests four instructional strategies—understanding argument structures, questioning, elaborating on structural knowledge, and assessing argumentation—for promoting medical …

Annual Financial Report, 2016-2017, University Of Arkansas, Fayetteville

Annual Financial Report

No abstract provided.

Isocitrate Dehydrogenase Mutation In Vibrio Anguillarum Results In Virulence Attenuation And Immunoprotection In Rainbow Trout (Oncorhynchus Mykiss), Xiangyu Mou, Edward J. Spinard, Shelby L. Hillman, David R. Nelson

Cell and Molecular Biology Faculty Publications

Background

Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depletion of required metabolic intermediates; however, it was not known whether mutations in central metabolism would affect virulence in an extracellular pathogen such as V. anguillarum.

Results

Seven central metabolism mutants were created and characterized with regard to growth in minimal and complex media, expression of virulence genes, and virulence in juvenile rainbow …

2017 November 14 - Medical Student Education Committee Minutes, Medical Student Education Committee, East Tennessee State University

Medical Student Education Committee Minutes

No abstract provided.

Method And System For Non-Invasive Optical Blood Glucose Detection Utilizing Spectral Data Analysis, Zhi Xu

UMSL Patents

Systems and methods are disclosed for non-invasively measuring blood glucose levels in a biological sample based on spectral data. This includes at least one light source configured to strike a target area of a sample, at least one light detector, which includes a preamplifier having a feedback resistor, positioned to receive light from the at least one light source and to generate an output signal, having a time dependent current, which is indicative of the power of light detected, and a processor configured to receive the output signal from the at least one light detector and based on the received …

An Undergraduate Laboratory Manual For Analyzing A Crispr Mutant With A Predicted Role In Regeneration, Susan Walsh, Ashley Becker, Paxton S. Sickler, Damian G. Clarke, Erin Jimenez

Faculty Publications

Exposing students to undergraduate research has reportedly improved students’ development of knowledge and skills in the laboratory, self-efficacy, satisfaction with their research, retention, and perseverance when faced with obstacles. Furthermore, utilizing authentic course-based undergraduate research experiences (CUREs) includes all students enrolled in the class, giving those who may not otherwise have access to an independent undergraduate research project an opportunity to engage in the scientific process in context of an original, unanswered question. In the fall of 2016, second semester introductory biology students conducted a semester-long research project on the transcription factor Lin28a to determine the effect of Lin28a on …

34th Annual Reception Bibliography, 2016-2017, Leonard H. Axe Library

Bibliographies

Since 1984, Leonard H. Axe Library at Pittsburg State University has honored book authors and other creators for their outstanding scholarship and contributions to their fields.

Research Excellence Recognized, Sarah Schaier

UNH Today Archive

No abstract provided.

Diatom Transcriptional And Physiological Responses To Changes In Iron Bioavailability Across Ocean Provinces, Natalie R. Cohen, Kelsey A. Ellis, Robert H. Lampe, Heather Mcnair, Benjamin S. Twining, Maria T. Maldonado, Mark A. Brzezinski, Fedor I. Kuzminov, Kimberlee Thamatrakoln, Claire P. Till, Kenneth W. Bruland, William G. Sunda, Sibel Bargu, Adrian Marchetti

Faculty Publications

Changes in iron (Fe) bioavailability influence diatom physiology and community composition, and thus have a profound impact on primary productivity and ecosystem dynamics. Iron limitation of diatom growth rates has been demonstrated in both oceanic and coastal waters of the Northeast Pacific Ocean and is predicted to become more pervasive in future oceans. However, it is unclear how the strategies utilized by phytoplankton to cope with low Fe bioavailability and resupply differ across these ocean provinces. We investigated the response of diatom communities to variable Fe conditions through incubation experiments performed in the Fe mosaic of the California Upwelling Zone …

Fbxw8-Dependent Degradation Of Mrfap1 In Anaphase Controls Mitotic Cell Death, Duan-Zhuo Li, Shun-Fang Liu, Lan Zhu, Yu-Xing Wang, Yi-Xiang Chen, Jie Liu, Gang Hu, Xin Yu, Jian Li, Jin Zhang, Zhi-Xiang Wu, Han Lu, Wei Liu, Bin Liu

Molecular and Cellular Biochemistry Faculty Publications

Mof4 family associated protein 1 (MRFAP1) is a 14 kDa nuclear protein, which involves in maintaining normal histone modification levels by negatively regulating recruitment of the NuA4 (nucleosome acetyltransferase of H4) histone acetyltransferase complex to chromatin. MRFAP1 has been identified as one of the most up-regulated proteins after NEDD8 (neural precursor cell expressed developmentally down-regulated 8) inhibition in multiple human cell lines. However, the biological function of MRFAP1 and the E3 ligase that targets MRFAP1 for destruction remain mysterious. Here we show, by using an immunoprecipitation-based proteomics screen, that MRFAP1 is an interactor of the F-box protein FBXW8. MRFAP1 is …

One- Vs. Three-Fraction Pancreatic Stereotactic Body Radiation Therapy For Pancreatic Carcinoma: Single Institution Retrospective Review, Philip Anthony Sutera, Mark E. Bernard, Beant S. Gill, Kamran K. Harper, Kimmen Quan, Nathan Bahary, Steven A. Burton, Herbert Zeh, Dwight E. Heron

Radiation Medicine Faculty Publications

Background/introduction: Early reports of stereotactic body radiation therapy (SBRT) for pancreatic ductal adenocarcinoma (PDAC) used single fraction, but eventually shifted to multifraction regimens. We conducted a single institution review of our patients treated with single- or multifraction SBRT to determine whether any outcome differences existed.

Methods and materials: Patients treated with SBRT in any setting for PDAC at our facility were included, from 2004 to 2014. Overall survival (OS), local control (LC), regional control (RC), distant metastasis (DM), and late grade 3 or greater radiation toxicities from the time of SBRT were calculated using Kaplan–Meier estimation to either the date …

2017-November-14-Morgan James, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

Finding A Key To Unlock Blocked Differentiation In Microrna-Deficient Embryonic Stem Cells, Jeff Hansen

Browse All News

No abstract provided.

Mperiod2Brdm1 And Other Single Period Mutant Mice Have Normal Food Anticipatory Activity, Julie S. Pendergast, Robert H. Wendroth, Rio C. Stenner, Charles D. Keil, Shin Yamazaki

Biology Faculty Publications

Animals anticipate the timing of food availability via the food-entrainable oscillator (FEO). The anatomical location and timekeeping mechanism of the FEO are unknown. Several studies showed the circadian gene, Period 2, is critical for FEO timekeeping. However, other studies concluded that canonical circadian genes are not essential for FEO timekeeping. In this study, we re-examined the effects of the Per2^Brdm1 mutation on food entrainment using methods that have revealed robust food anticipatory activity in other mutant lines. We examined food anticipatory activity, which is the output of the FEO, in single Period mutant mice. Single Per1, Per2 …

Gardner-Webb Students Compete In Worldquest, Office Of University Communications

Gardner-Webb NewsCenter Archive

A team of students from Gardner-Webb University recently tested their knowledge of world facts by competing in WorldQuest in Charlotte, N.C. The group of political science, global studies, history and biology majors were accompanied by Dr. Casey Delehanty, assistant professor of Global Studies. Sponsored by the World Affairs Council of Charlotte, the event included approximately 600 students and community members from all around the greater Charlotte area.

A Comprehensive Patient-Derived Xenograft Collection Representing The Heterogeneity Of Melanoma., Clemens Krepler, Katrin Sproesser, Patricia Brafford, Marilda Beqiri, Bradley Garman, Min Xiao, Batool Shannan, Andrea Watters, Michela Perego, Gao Zhang, Adina Vultur, Xiangfan Yin, Qin Liu, Ioannis N Anastopoulos, Bradley Wubbenhorst, Melissa A. Wilson, Wei Xu, Giorgos Karakousis, Michael Feldman, Xiaowei Xu, Ravi Amaravadi, Tara C. Gangadhar, David E. Elder, Lauren E. Haydu, Jennifer A. Wargo, Michael A. Davies, Yiling Lu, Gordon B. Mills, Dennie T. Frederick, Michal Barzily-Rokni, Keith T. Flaherty, Dave S. Hoon, Michael Guarino, Joseph J. Bennett, Randall W. Ryan, Nicholas J. Petrelli, Carol L. Shields, Mizue Terai, Takami Sato, Andrew E. Aplin, Alexander Roesch, David Darr, Steve Angus, Rakesh Kumar, Ensar Halilovic, Giordano Caponigro, Sebastien Jeay, Jens Wuerthner, Annette Walter, Matthias Ocker, Matthew B. Boxer, Lynn Schuchter, Katherine L Nathanson, Meenhard Herlyn

Kimmel Cancer Center Papers, Presentations, and Grand Rounds

Therapy of advanced melanoma is changing dramatically. Following mutational and biological subclassification of this heterogeneous cancer, several targeted and immune therapies were approved and increased survival significantly. To facilitate further advancements through pre-clinical in vivo modeling, we have established 459 patient-derived xenografts (PDX) and live tissue samples from 384 patients representing the full spectrum of clinical, therapeutic, mutational, and biological heterogeneity of melanoma. PDX have been characterized using targeted sequencing and protein arrays and are clinically annotated. This exhaustive live tissue resource includes PDX from 57 samples resistant to targeted therapy, 61 samples from responders and non-responders to immune checkpoint …

The Gene Smart Study: Method, Study Design, And Preliminary Findings, Xu Yan, Nir Eynon, Ioannis D. Papadimitriou, Jujiao Kuang, Fiona Munson, Oren Tirosh, Lannie O’Keefe, Lyn R. Griffiths, Kevin J. Ashton, Nuala Byrne, Yannis P. Pitsiladis, David J. Bishop

Research outputs 2014 to 2021

The gene SMART (genes and the Skeletal Muscle Adaptive Response to Training) Study aims to identify genetic variants that predict the response to both a single session of High-Intensity Interval Exercise (HIIE) and to four weeks of High-Intensity Interval Training (HIIT). While the training and testing centre is located at Victoria University, Melbourne, three other centres have been launched at Bond University, Queensland University of Technology, Australia, and the University of Brighton, UK. Currently 39 participants have already completed the study and the overall aim is to recruit 200 moderately-trained, healthy Caucasians participants (all males 18 – 45 y, BMI …

Evaluating The Effect Of Chitosan, Water-Soluble Chitosan And Chitosan Application Techniques On The Quality Of Refrigerated Catfish Fillets, Franklin Bonilla Torres

LSU Master's Theses

Chitosan (CH) is a biopolymer synthetized by deacetylation of chitin, it is found in the exoskeleton of crustaceans, cell wall of fungi, and insects. CH’s water insolubility limited the used of this compound in some food systems. However, enzymatic hydrolysis of CH yields water-soluble chitosan (WSC). The aim of this study was to evaluate the effect of chitosan, water-soluble chitosan, and chitosan application techniques on the quality of refrigerated catfish fillets. This research was conducted in two separated studies. In the first study, three solution treatments were prepared: (1) a 1% acetic acid (AA) solution, (2) a 0.5% CH solution …