Digital Commons Network^™

The Impact Of The Bovine Faecal Microbiome On Escherichia Coli O157:H7 Prevalence And Enumeration In Naturally Infected Cattle, M. Kim, L. A. Kuehn, J. L. Bono, E. D. Berry, N. Kalchayanand, H. C. Freetly, A. K. Benson, J. E. Wells

Department of Food Science and Technology: Faculty Publications

Aims: The objective of this study was to determine if the faecal microbiome has an association with Escherichia coli O157:H7 prevalence and enumeration.

Methods and Results: Pyrosequencing analysis of faecal microbiome was performed from feedlot cattle fed one of three diets: (i) 94 heifers fed low concentrate (LC) diet, (ii) 142 steers fed moderate concentrate (MC) diet, and (iii) 132 steers fed high concentrate (HC) diet. A total of 322 585 OTUs were calculated from 2,411,122 high-quality sequences obtained from 368 faecal samples. In the LC diet group, OTUs assigned to the orders Clostridiales and RF39 (placed within …

Evaluating Potential Risks Of Food Allergy And Toxicity Of Soy Leghemoglobin Expressed In Pichia Pastoris, Yuan Jin, Xiaoyun He, Kwame Andoh-Kumi, Rachel Z. Fraser, Mei Lu, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Scope: The Soybean (Glycine max) leghemoglobin c2 (LegHb) gene was introduced into Pichia pastoris yeast for sustainable production of a heme-carrying protein, for organoleptic use in plant-based meat. The potential allergenicity and toxicity of LegHb and 17 Pichia host-proteins each representing ≥ 1% of total protein in production batches are evaluated by literature review, bioinformatics sequence comparisons to known allergens or toxins, and in vitro pepsin digestion.

Methods and results: Literature searches found no evidence of allergenicity or toxicity for these proteins. There are no significant sequence matches of LegHb to known allergens or toxins. Eleven Pichia proteins have modest …

Next-Generation Probiotics Targeting Clostridium Difficile Through Precursor- Directed Antimicrobial Biosynthesis, Jennifer K. Spinler, Jennifer Auchtung, Aaron Brown, Prapaporn Boonma, Numan Oezguen, Cana L. Ross, Ruth Ann Luna, Jessica Runge, James Versalovic, Alex Peniche, Sara M. Dann, Robert A. Britton, Anthony Haag, Tor C. Savidge

Department of Food Science and Technology: Faculty Publications

Integration of antibiotic and probiotic therapy has the potential to lessen the public health burden of antimicrobial-associated diseases. Clostridium difficile infection (CDI) represents an important example where the rational design of next-generation probiotics is being actively pursued to prevent disease recurrence. Because intrinsic resistance to clinically relevant antibiotics used to treat CDI (vancomycin, metronidazole, and fidaxomicin) is a desired trait in such probiotic species, we screened several bacteria and identified Lactobacillus reuteri to be a promising candidate for adjunct therapy. Human-derived L. reuteri bacteria convert glycerol to the broad-spectrum antimicrobial compound reuterin. When supplemented with glycerol, strains carrying the pocR …

The Gut Mycobiome Of The Human Microbiome Project Healthy Cohort, Andrea K. Nash, Thomas A. Auchtung, Matthew C. Wong, Daniel P. Smith, Jonathan R. Gesell, Matthew C. Ross, Christopher J. Stewart, Ginger A. Metcalf, Donna M. Muzny, Richard A. Gibbs, Nadim J. Ajami, Joseph F. Petrosino

Department of Food Science and Technology: Faculty Publications

Background: Most studies describing the human gut microbiome in healthy and diseased states have emphasized the bacterial component, but the fungal microbiome (i.e., the mycobiome) is beginning to gain recognition as a fundamental part of our microbiome. To date, human gut mycobiome studies have primarily been disease centric or in small cohorts of healthy individuals. To contribute to existing knowledge of the human mycobiome, we investigated the gut mycobiome of the Human Microbiome Project (HMP) cohort by sequencing the Internal Transcribed Spacer 2 (ITS2) region as well as the 18S rRNA gene.

Results: Three hundred seventeen HMP stool samples were …

Lifestyle And Horizontal Gene Transfer- Mediated Evolution Of Mucispirillum Schaedleri, A Core Member Of The Murine Gut Microbiota, Alexander Loy, Carina Pfann, Michaela Steinberger, Buck Hanson, Simone Herp, Sandrine Brugiroux, João Carlos Gomes Neto, Mark V. Boekschoten, Clarissa Schwab, Tim Urich, Amanda E. Ramer-Tait, Thomas Rattei, Bärbel Stecher, David Berry

Department of Food Science and Technology: Faculty Publications

Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, …

Young Children With Down Syndrome Show Normal Development Of Circadian Rhythms, But Poor Sleep Efficiency: A Cross-Sectional Study Across The First 60 Months Of Life, Fabian Fernandez, Casandra C. Nyhuis, Payal Anand, Bianca I. Demara, Norman F. Ruby, Goffredina Spano, Caron Clark, Jamie O. Edgin

Department of Educational Psychology: Faculty Publications

Objectives: To evaluate sleep consolidation and circadian activity rhythms in infants and toddlers with Down syndrome (DS) under light and socially entrained conditions within a familiar setting. Given previous human and animal data suggesting intact circadian regulation of melatonin across the day and night, it was hypothesized that behavioral indices of circadian rhythmicity would likewise be intact in the sample with DS.

Methods: A cross-sectional study of 66 infants and young children with DS, aged 5e67 months, and 43 typically developing age-matched controls. Sleep and measures of circadian robustness or timing were quantified using continuous in-home actigraphy recordings performed over …

Citation Network Analysis Of Science Doctoral Theses In The Faculty Of Science, University Of Ibadan, Malik Oyewale Salami Mr, Wole Michael Olatokun Prof.

Library Philosophy and Practice (e-journal)

Objective - This paper investigated the intellectual structure of the cited journals by science doctoral students in the University of Ibadan, Nigeria through field-mapping mechanism namely co-citation analysis. The study also explored the relationship and its strength between the science disciplines in terms of journals that were co-referenced through bibliographic coupling analysis. The deployment of bibliographic coupling techniques with co-citation analysis was adopted to model the pattern of scientific communications among scientists and scholarly journals.

Methods - The scholarly citation data were collected from science doctoral theses submitted between 2006 and 2013 across ten disciplines in the Faculty of Science, …

Low-Drag Events In Transitional Wall-Bounded Turbulence, Richard D. Whalley, Jae Sung Park, Anubhav Kushwaha, David J.C. Dennis, Michael D. Graham, Robert J. Poole

Department of Mechanical and Materials Engineering: Faculty Publications

Intermittency of low-drag pointwise wall shear stress measurements within Newtonian turbulent channel flow at transitional Reynolds numbers (friction Reynolds numbers 70 – 130) is characterized using experiments and simulations. Conditional mean velocity profiles during low-drag events closely approach that of a recently discovered nonlinear traveling wave solution; both profiles are near the so-called maximum drag reduction profile, a general feature of turbulent flow of liquids containing polymer additives (despite the fact that all results presented are for Newtonian fluids only). Similarities between temporal intermittency in small domains and spatiotemporal intermittency in large domains is thereby found.

Flowtaxis Of Osteoblast Migration Under Fluid Shear And The Effect Of Rhoa Kinase Silencing, Brandon D. Riehl, Jeong Soon Lee, Ligyeom Ha, Il Keun Kwon, Jung Yul Lim

Department of Mechanical and Materials Engineering: Faculty Publications

Despite the important role of mechanical signals in bone remodeling, relatively little is known about how fluid shear affects osteoblastic cell migration behavior. Here we demonstrated that MC3T3-E1 osteoblast migration could be activated by physiologically-relevant levels of fluid shear in a shear stress-dependent manner. Interestingly, shear-sensitive osteoblast migration behavior was prominent only during the initial period after the onset of the steady flow (for about 30 min), exhibiting shear stress-dependent migration speed, displacement, arrest coefficient, and motility coefficient. For example, cell speed at 1 min was 0.28, 0.47, 0.51, and 0.84 μm min^-1 for static, 2, 15, and 25 …

Fluid-Structure Interaction In Abdominal Aortic Aneurysm: Effect Of Modeling Techniques, Shengmao Lin, Xinwei Han, Yonghua Bi, Siyeong Ju, Linxia Gu

Department of Mechanical and Materials Engineering: Faculty Publications

In this work, the impact of modeling techniques on predicting the mechanical behaviors of abdominal aortic aneurysm (AAA) is systematically investigated. The fluid-structure interaction (FSI) model for simultaneously capturing the transient interaction between blood flow dynamics and wall mechanics was compared with its simplified techniques, that is, computational fluid dynamics (CFD) or computational solid stress (CSS) model. Results demonstrated that CFD exhibited relatively smaller vortexes and tends to overestimate the fluid wall shear stress, compared to FSI. On the contrary, the minimal differences in wall stresses and deformation were observed between FSI and CSS models. Furthermore, it was found that …

Molecular Dynamics Simulations Of Heterogeneous Cell Membranes In Response To Uniaxial Membrane Stretches At High Loading Rates, Lili Zhang, Zesheng Zhang, John Jasa, Dongli Li, Robin O. Cleveland, Mehrdad Negahban, Antoine Jérusalem

Department of Mechanical and Materials Engineering: Faculty Publications

The chemobiomechanical signatures of diseased cells are often distinctively different from that of healthy cells. This mainly arises from cellular structural/compositional alterations induced by disease development or therapeutic molecules. Therapeutic shock waves have the potential to mechanically destroy diseased cells and/or increase cell membrane permeability for drug delivery. However, the biomolecular mechanisms by which shock waves interact with diseased and healthy cellular components remain largely unknown. By integrating atomistic simulations with a novel multiscale numerical framework, this work provides new biomolecular mechanistic perspectives through which many mechanosensitive cellular processes could be quantitatively characterised. Here we examine the biomechanical responses of …

A Rapid And Non-Invasive Method For Measuring The Peak Positive Pressure Of Hifu Fields By A Laser Beam, Hua Wang, Deping Zeng, Ziguang Chen, Zengtao Yang

Department of Mechanical and Materials Engineering: Faculty Publications

Based on the acousto-optic interaction, we propose a laser deflection method for rapidly, non-invasively and quantitatively measuring the peak positive pressure of HIFU fields. In the characterization of HIFU fields, the effect of nonlinear propagation is considered. The relation between the laser deflection length and the peak positive pressure is derived. Then the laser deflection method is assessed by comparing it with the hydrophone method. The experimental results show that the peak positive pressure measured by laser deflection method is little higher than that obtained by the hydrophone, confirming that they are in reasonable agreement. Considering that the peak pressure …

Growth Mechanisms Of Multiscale, Mound-Like Surface Structures On Titanium By Femtosecond Laser Processing, Edwin Peng, Ryan Bell, Craig A. Zuhlke, Meiyu Wang, Dennis R. Alexander, George Gogos, Jeffrey E. Shield

Department of Mechanical and Materials Engineering: Faculty Publications

Femtosecond laser surface processing (FLSP) can be used to functionalize many surfaces, imparting specialized properties such as increased broadband optical absorption or superhydrophobicity/-hydrophilicity. In this study, the subsurface microstructure of a series of mound-like FLSP structures formed on commercially pure titanium using five combinations of laser fluence and cumulative pulse counts was studied. Using a dual beam Scanning Electron Microscope with a Focused Ion Beam, the subsurface microstructure for each FLSP structure type was revealed by cross-sectioning. The microstructure of the mounds formed using the lowest fluence value consists of the original Ti grains. This is evidence that preferential laser …

Design And Field Testing Of A Combined Flaming And Cultivation Implement For Effective Weed Control, Brian D. Neilson, Chris A. Bruening, Strahinja Stepanovic, Avishek Datta, Stevan Z. Knezevic, George Gogos

Department of Mechanical and Materials Engineering: Faculty Publications

Flaming is a thermal weed control method that uses heat to control weeds within or between crop rows. Mechanical cultivation is another weed control method which undercuts weeds between crop rows, while leaving a strip of weeds within the crop row. A combination flamer/cultivator implement was designed to take advantage of the good qualities of both flaming and cultivation methods to provide a more consistent weed control than using either flaming or mechanical cultivation alone. Flaming hoods were designed in the spring of 2010 and retrofitted on an existing row crop cultivator. The flaming hoods were redesigned in the spring …

Robotic Surgical Devices, Systems, And Related Methods, Shane M. Farritor, Erik Mumm, Philip Chu, Nishant Kumar, Jason Dumpert, Yutaka Tsutano

Department of Mechanical and Materials Engineering: Faculty Publications

The embodiments disclosed herein relate to various medical device components, including components that can be incorporated into robotic and/or in vivo medical devices. Certain embodiments include various modular medical devices for in vivo medical procedures.

Methods , Systems , And Devices Relating To Surgical End Effectors, Shane M. Farritor, Tom Fredrick, Joe Bartels

Department of Mechanical and Materials Engineering: Faculty Publications

The embodiments disclosed herein relate to various medical device components, including components that can be incorporated into robotic and/or in vivo medical devices, and more specifically including end effectors that can be incorporated into such devices. Certain end effector embodiments include various vessel cautery devices that have rotational movement as well as cautery and cutting functions while maintaining a relatively compact structure. Other end effector embodiments include various end effector devices that have more than one end effector.

Biodegradable Elastic Nanofibrous Platforms With Integrated Flexible Heaters For On-Demand Drug Delivery, Ali Tamayol, Alireza Hassani Najafabadi, Pooria Mostafalu, Ali K. Yetisen, Mattia Commotto, Musab Aldhahri, Mohamed Shaaban Abdel-Wahab, Zeynab Izadi Najafabadi, Shahrzad Latifi, Mohsen Akbari, Nasim Annabi, Seok Hyun Yun, Adnan Memic, Mehmet R. Dokmeci, Ali Khademhosseini

Department of Mechanical and Materials Engineering: Faculty Publications

Delivery of drugs with controlled temporal profiles is essential for wound treatment and regenerative medicine applications. For example, bacterial infection is a key challenge in the treatment of chronic and deep wounds. Current treatment strategies are based on systemic administration of high doses of antibiotics, which result in side effects and drug resistance. On-demand delivery of drugs with controlled temporal profile is highly desirable. Here, we have developed thermally controllable, antibiotic-releasing nanofibrous sheets. Poly(glycerol sebacate)- poly(caprolactone) (PGS-PCL) blends were electrospun to form elastic polymeric sheets with fiber diameters ranging from 350 to 1100 nm and substrates with a tensile modulus …

Nondestructive Testing System Design For Biological Product Based On Vibration Signal Analysis Of Acceleration Sensor, Xiaohao Li, Tao Shen

Department of Mechanical and Materials Engineering: Faculty Publications

In order to reduce the disadvantages of current biological product quality testing methods, taking the quality testing in cocoon trade markets as an example, this paper has proposed a quality nondestructive testing method for biological products based on the analysis of vibration signal from acceleration sensors. According to the wavelet transformation analysis on the random vibration signal acquired from the acceleration sensor, the random vibration signal related to the silkworm chrysalis quality has been analyzed and reconstructed; then the characteristic values such as: mean value, variance, mean square root, waveform index, pulse factor, and so on of the quality signal …

Reactivation Of Hiv-1 Proviruses In Immune-Compromised Mice Engrafted With Human Voa-Negative Cd4+ T Cells, Zhe Yuan, Guobin Kang, Wuxun Lu, Qingsheng Li

Nebraska Center for Virology: Faculty Publications

Background: HIV-1 infection remains incurable on antiretroviral therapy (ART) due to virus latency. To date, enhanced co-culture assays, including viral outgrowth assays (VOA), are commonly used to measure HIV-1 latent reservoirs and evaluate latency-reversing agents (LRAs). However, VOA can only reactivate a small fraction of intact proviruses.

Methods: To explore the utility of NOD scid gamma (NSG) mice as an in vivo model to reactivate HIV-1 proviruses from VOA-negative CD4+ T cells, resting CD4+ T cells from an HIV-1 latently infected individual were isolated and the human CD4+ T cells corresponding to VOA-positive and VOA-negative CD4+ T cells were engrafted …

Mass Political Behavior, Ingrid J. Haas, Stephen P. Schneider

Department of Political Science: Faculty Publications

Mass political behavior is the study of how average citizens form and express opinions about politics and decide how to engage with the political system through voting or other forms of political participation. Political scientists interested in mass political behavior have drawn on a variety of disciplinary approaches to understand the topic, including history, economics, sociology, and more recently, psychology, biology, and neuroscience. Political psychologists interested in understanding mass political behavior have applied social psychological theories of attitudes, emotion, social cognition, and social identity to help improve our understanding of political behavior. This entry provides a brief overview of how …

Genes Linked To Production Of Secondary Metabolites In Talaromyces Atroroseus Revealed Using Crispr-Cas9, Maria Lund Nielsen, Thomas Isbrandt, Kasper Bøwig Rasmussen, Ulf Thrane, Jakob Blæsbjerg Hoof, Thomas Ostenfeld Larsen, Uffe Hasbro Mortensen

Department of Plant Pathology: Faculty Publications

The full potential of fungal secondary metabolism has until recently been impeded by the lack of universal genetic tools for most species. However, the emergence of several CRISPR-Cas9-based genome editing systems adapted for several genera of filamentous fungi have now opened the doors for future efforts in discovery of novel natural products and elucidation and engineering of their biosynthetic pathways in fungi where no genetic tools are in place. So far, most studies have focused on demonstrating the performance of CRISPR-Cas9 in various fungal model species, and recently we presented a versatile CRISPR-Cas9 system that can be successfully applied in …

Muti Site Screening Identifies And Verifies Levels Of Resistance To White Mold In Common Bean In 2016, Robert Higgins, James R. Steadman

Department of Plant Pathology: Faculty Publications

The development of common bean cultivars with partial resistance and/ or avoidance to white mold (WM) caused by Sclerotinia sclerotiorum would benefit producers by reducing yield loss and reducing input costs for fungicides. Our main objective in this study is to identify bean germplasm supplied by bean breeders from across the USA and Belgium with levels of partial resistance to WM. Breeders sent seed of 9 bean lines for field testing and 25 bean lines for greenhouse testing with putative sources of resistance to our laboratory. The seeds were divided in equal amounts for field (400g/line) and/or greenhouse (25 seeds/ …

Wheat Streak Mosaic Virus Coat Protein Is A Host-Specific Long-Distance Transport Determinant In Oat, Satyanarayana Tatineni

Department of Plant Pathology: Faculty Publications

Viral determinants involved in systemic infection of hosts by monocot-infecting plant viruses are poorly understood. Wheat streak mosaic virus (WSMV, genus Tritimovirus, family Potyviridae) exclusively infects monocotyledonous crops such as wheat, oat, barley, maize, triticale, and rye. Previously, we reported that WSMV CP amino acids 36–84 are expendable for systemic infection of wheat, maize, barley and rye. In this study, the role of coat protein (CP) in systemic infection of oat by WSMV was examined by using a series of viable deletion mutants. WSMV bearing deletions within or encompassing all of amino acids 36–57 efficiently infected oat, indicating that …

Understanding Fungicide Resistance, Tamra A. Jackson-Ziems, Loren J. Giesler, Anthony O. Adesemoye, Robert M. Harveson, Stephen N. Wegulo

Department of Plant Pathology: Faculty Publications

Fungicide resistance has developed in some diseases of row crops as well as specialty crops. This implies that fungicide applications to control such diseases may no longer be effective. Scientific studies have shown that fungicide resistance develops through natural selection of a mutant strain of a pathogen in a population that is resistant to fungicides. Resistance is very difficult to eliminate but can be delayed through appropriate management practices. The availability of inexpensive options with single mode of action products being available makes this an important issue so we do not repeat what was done in weed management.

Introduction

Frogeye …

Population Structure And Phenotypic Variation Of Sclerotinia Sclerotiorum From Dry Bean (Phaseolus Vulgaris) In The United States, Zhian N. Kamvar, Bimal Sajeewa Amaradasa, Rachana Jhala, Serena Mccoy, James R. Steadman, Sydney Everhart

Department of Plant Pathology: Faculty Publications

The ascomycete pathogen Sclerotinia sclerotiorum is a necrotrophic pathogen on over 400 known host plants, and is the causal agent of white mold on dry bean. Currently, there are no known cultivars of dry bean with complete resistance to white mold. For more than 20 years, bean breeders have been using white mold screening nurseries (wmn) with natural populations of S. sclerotiorum to screen new cultivars for resistance. It is thus important to know if the genetic diversity in populations of S. sclerotiorum within these nurseries (a) reflect the genetic diversity of the populations in the surrounding region and (b) …

Pseudopecoelus Mccauleyi N. Sp. And Podocotyle Sp. (Digenea: Opecoelidae) From The Deep Waters Off Oregon And British Columbia With An Updated Key To The Species Of Pseudopecoelus Von Wicklen, 1946 And Checklist Of Parasites From Lycodes Cortezianus (Perciformes: Zoarcidae), Charles K. Blend, Norman O. Dronen, Gábor R. Rácz, Scott Lyell Gardner

Scott L. Gardner Publications

Pseudopecoelus mccauleyi n. sp. (Opecoelidae: Opecoelinae) is described from the intestine of the bigfin eelpout, Lycodes cortezianus (Gilbert, 1890) (Perciformes: Zoarcidae), collected at 200–800 m depths in the northeastern Pacific Ocean off Oregon and Vancouver Island, British Columbia. The new species is distinguished by possessing a unique combination of the following diagnostic characters: vitelline fields that extend to the posterior margin of the ventral sucker; a slender, tubular and sinuous seminal vesicle that extends some distance into the hindbody; an unspecialized, protuberant ventral sucker; a genital pore at pharynx level; lobed to deeply multilobed testes; a lobed ovary; and an …

A New Species Of Catenotaenia (Cestoda: Catenotaeniidae) From Pygeretmus Pumilio Kerr, 1792 From The Gobi Of Mongolia, Altangerel Tsogtsaikhan Dursahinhan, Batsaikhan Nyamsuren, Danielle Marie Tufts, Scott Lyell Gardner

Scott L. Gardner Publications

From 1999 through 2012, a total of 541 individual rodents (jerboas of the family Dipodidae) were collected from several habitat types, primarily from the Gobi region of Mongolia, and were examined for helminth and protistan parasites. Of those rodents, 25 were identified as Pygeretmus pumilio Kerr, 1792 (Rodentia: Dipodidae), whereas 516 were other species of jerboa from the provinces of Dornogobi, Dundgobi, Omnogobi, Ovorhangai, Bayanhongor, Gobi Altai, and Hovd. During our field work, we collected several cestodes; some of which represented undescribed species, and these new species occurred in 40% of P. pumilio from four separate collecting localities. We designate …

Nematodes Associated With Mammals In The Great American Biotic Interchange (Gabi), F. Agustin Jimenez, Juliana Notarnicola, Scott Lyell Gardner

Scott L. Gardner Publications

The Great American Biotic Interchange (GABI) is a large-scale zoogeographic event that illustrates the exchange and diversification of mammals between North and South America. This phenomenon was accelerated by the connection of both landmasses during the Pliocene. Support for this phenomenon includes the extant distribution of xenarthrans, didelphiomorph marsupials, hystricognath and cricetine rodents, sciurids and carnivores, as well as the distribution of fossils in the stratigraphic record and the coalescence of genotypes. Contrasting with the relatively well-documented role and history of mammals in GABI, the role of their parasites has been largely neglected. As a consequence, the reconstructions of the …

Rapid Typing Of Mannheimia Haemolytica Major Genotypes 1 And 2 Using Maldi-Tof Mass Spectrometry, John Dustin Loy, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.

Efficacy Of Urtoxazumab (Tma-15 Humanized Monoclonal Antibody Specific For Shiga Toxin 2) Against Post-Diarrheal Neurological Sequelae Caused By Escherichia Coli O157:H7 Infection In The Neonatal Gnotobiotic Piglet Model, Rodney A. Moxley, David H. Francis, Mizuho Tamura, David B. Marx, Kristina Santiago-Mateo, Mojun Zhao

School of Veterinary and Biomedical Sciences: Faculty Publications

Enterohemorrhagic Escherichia coli (EHEC) is the most common cause of hemorrhagic colitis and hemolytic uremic syndrome in human patients, with brain damage and dysfunction the main cause of acute death. We evaluated the efficacy of urtoxazumab (TMA-15, Teijin Pharma Limited), a humanized monoclonal antibody against Shiga toxin (Stx) 2 for the prevention of brain damage, dysfunction, and death in a piglet EHEC infection model. Forty-five neonatal gnotobiotic piglets were inoculated orally with 3 x 10⁹ colony-forming units of EHEC O157:H7 strain EDL933 (Stx1⁺, Stx2⁺) when 22–24 h old. At 24 h post-inoculation, piglets were intraperitoneally …