Digital Commons Network^™

Farmers’ Perspectives On Resistance In Western Corn Rootworm To Crw-Bt Corn In Midwest Usa, David A. Andow, Robert Wright, Erin W. Hodgson, Thomas E. Hunt, Ken Ostlie

Department of Entomology: Faculty Publications

Resistance in western corn rootworm to transgenic corn hybrids was first confirmed in 2011 in Midwestern USA, and threatens their continued use. Farmers are often the first line of resistance detection, so their understanding and attitudes toward this issue are critical for improving resistance management. We conducted telephone focus groups during 2013 with farmers who had experienced rootworm resistance. There were four stages in dealing with unexpected rootworm injury: Awareness of a problem, diagnosis, confirmation, and recommendations. Most farmers discovered the problem themselves, but this usually happened too late in the growing season to limit yield loss. Once aware of …

Obituary And Bibliography Of Norm Penny (1946-2016), An “Entomologist’S Entomologist”, Robert L. Zuparko, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

The section “Remembrances of Norm Penny” was authored by BCR, and the remaining sections by RLZ. Fabio Penny and Rachel Diaz-Bastin made valuable additions to the text.

Norman Dale Penny, collection manager emeritus in the Department of Entomology at the California Academy of Sciences, San Francisco, California, and past president of the Pacifi c Coast Entomological Society, died of a stroke on July 10, 2016. That weekend he and his wife were near Quincy, in Plumas County, California, collecting Mecoptera for DNA retrieval to help elucidate the phylogeny of the group. After collecting his targeted species, he suffered a severe …

Using Publicly Available Data To Quantify Plant–Pollinator Interactions And Evaluate Conservation Seeding Mixes In The Northern Great Plains, Clint R.V. Otto, S. O'Dell, R. B. Bryant, N. H. Euliss Jr., R. M. Bush, Matthew D. Smart

Department of Entomology: Faculty Publications

Concern over declining pollinators has led to multiple conservation initiatives for improving forage for bees in agroecosystems. Using data available through the Pollinator Library (npwrc.usgs.gov/pollinator/), we summarize plant–pollinator interaction data collected from 2012–2015 on lands managed by the U.S. Fish and Wildlife Service and private lands enrolled in U.S. Department of Agriculture conservation programs in eastern North Dakota (ND). Furthermore, we demonstrate how plant–pollinator interaction data from the Pollinator Library and seed cost information can be used to evaluate hypothetical seeding mixes for pollinator habitat enhancements. We summarize records of 314 wild bee and 849 honey bee (Apis mellifera …

Thiamethoxam Toxicity And Effects On Consumption Behavior In Orius Insidiosus (Hemiptera: Anthocoridae) On Soybean, Carolina Camargo, Thomas Hunt, Loren J. Giesler, Blair D. Siegfried

Department of Entomology: Faculty Publications

Neonicotinoid residues can be present in soybean vegetative tissue, prey insects, and flower tissues, possibly making them toxic to pollinators and natural enemies. Baseline information on the toxicity of neonicotinoids to beneficial insects other than pollinators through multiple routes of insecticide exposure is limited. The objectives of this study were 1) to evaluate the toxicity of thiamethoxam to the hemipteran predator, Orius insidiosus Say, exposed to residues through treated vegetative tissue and insect prey, and 2) to evaluate the effect of thiamethoxam on the abundance of this predator species in soybean fields. Predators were exposed to thiamethoxam in soybean leaves …

Atp-Sensitive Inwardly Rectifying Potassium Channel Regulation Of Viral Infections In Honey Bees, Scott T. O'Neal, Daniel R. Swale, Troy D. Anderson

Department of Entomology: Faculty Publications

Honey bees are economically important pollinators of a wide variety of crops that have attracted the attention of both researchers and the public alike due to unusual declines in the numbers of managed colonies in some parts of the world. Viral infections are thought to be a significant factor contributing to these declines, but viruses have proven a challenging pathogen to study in a bee model and interactions between viruses and the bee antiviral immune response remain poorly understood. In the work described here, we have demonstrated the use of flock house virus (FHV) as a model system for virus …

Weight Of Evidence Evaluation Of A Network Of Adverse Outcome Pathways Linking Activation Of The Nicotinic Acetylcholine Receptor In Honey Bees To Colony Death, Carlie A. Lalone, Daniel L. Villeneuve, Judy Wu-Smart, Rebecca Y. Milsk, Keith Sappington, Kristina V. Garber, Justin Housenger, Gerald T. Ankley

Department of Entomology: Faculty Publications

Ongoing honey bee (Apis mellifera) colony losses are of significant international concern because of the essential role these insects play in pollinating crops. Both chemical and non-chemical stressors have been implicated as possible contributors to colony failure; however, the potential role(s) of commonly-used neonicotinoid insecticides has emerged as particularly concerning. Neonicotinoids act on the nicotinic acetylcholine receptors (nAChRs) in the central nervous system to eliminate pest insects. However, mounting evidence indicates that neonicotinoids also may adversely affect beneficial pollinators, such as the honey bee, via impairments on learning and memory, and ultimately foraging success. The specific mechanisms linking …

Wheat Streak Mosaic Virus Coat Protein Is A Determinant For Vector Transmission By The Wheat Curl Mite, Satyanarayana Tatineni, Anthony J. Mcmechan, Gary L. Hein

Department of Entomology: Faculty Publications

Wheat streak mosaic virus (WSMV; genus Tritimovirus; family Potyviridae), is transmitted by the wheat curl mite (Aceria tosichella Keifer). The requirement of coat protein (CP) for WSMV transmission by the wheat curl mite was examined using a series of viable deletion and point mutations. Mite transmission of WSMV was completely abolished with deletions comprising CP amino acids 58–100. In contrast, the amino-proximal (amino acids 6–27 and 36–57) and carboxy-terminal (14 amino acids) regions of CP were expendable for mite transmission. Mutation of aspartic acid residues at amino acid positions 289 or 326 (D289A or D326A) at the …

Acute-Contact And Chronic-Systemic In Vivo Bioassays: Regional Monitoring Of Susceptibility To Thiamethoxam In Soybean Aphid (Hemiptera: Aphididae) Populations From The North Central United States, Matheus G.P.M. Ribeiro, Thomas E. Hunt, Blair Siegfried

Department of Entomology: Faculty Publications

The risks associated with soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), in the North Central soybean systems has fostered the adoption of prophylactic chemical control practices, such as planting seeds treated with neonicotinoid insecticides, especially thiamethoxam. Consequently, there is a concern that increased selection pressure imposed on the arthropod–pest complex by this insecticide may lead to resistance. Therefore, in vivo bioassays were conducted to determine the susceptibility of soybean aphid to thiamethoxam among North Central U.S. populations. Concentration-mortality data were collected using contact glass-vial and detached-leaf systemic bioassays. The results of these experiments indicate that both bioassays were reliable to …

Sublethal Effects Of Vatpase-A And Snf7 Dsrnas On Biology Of Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi Barber, Adriano E. Pereira, Ana María Vélez, Lance J. Meinke, Blair D. Siegfried

Department of Entomology: Faculty Publications

RNA interference is a powerful tool against corn rootworm. Adults and neonates of southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), were exposed to the LC₅₀ of vATPase-A and Snf7 double-stranded RNAs (dsRNAs), and the effects on female fecundity, egg viability, male fitness as measured by sperm viability and mating capacity, larval recovery along with dry weight, and instar determination 10 d after exposure to dsRNA, were determined. Significant reductions were observed for a number of parameters in dsRNA-exposed rootworms relative to control treatments. Female fecundity and larval recovery were significantly reduced after exposure to both dsRNAs. In …

Evaluation Of Smartstax And Smartstax Pro Maize Against Western Corn Rootworm And Northern Corn Rootworm: Efficacy And Resistance Management, Graham P. Head, Matthew W. Carroll, Sean P. Evans, Dwain M. Rule, Alan R. Willse, Thomas L. Clark, Nicholas P. Storer, Ronald D. Flannagan, Luke W. Samuel, Lance Meinke

Department of Entomology: Faculty Publications

Background: Cases of western corn rootworm (WCR) field-evolved resistance to Cry3Bb1 and other corn rootworm (CRW) control traits have been reported. Pyramid products expressing multiple CRW traits can delay resistance compared to single trait products. We used field studies to assess the pyramid CRW corn products, SmartStax (expressing Cry3Bb1 and Cry34Ab1/Cry35Ab1) and SmartStax PRO (expressing Cry3Bb1, Cry34Ab1/Cry35Ab1 and DvSnf7), at locations with high WCR densities and possible Cry3Bb1 resistance, and to assess the reduction in adult emergence attributable to DvSnf7 and other traits. Insect resistance models were used to assess durability of SmartStax and SmartStax PRO to WCR resistance.

Results: …

Identification, Biology, Impacts, And Management Of Stink Bugs (Hemiptera: Heteroptera: Pentatomidae) Of Soybean And Corn In The Midwestern United States, Robert L. Koch, Daniela T. Pezzini, Andrew P. Michel, Thomas Hunt

Department of Entomology: Faculty Publications

Stink bugs (Hemiptera: Heteroptera: Pentatomidae) are an emerging threat to soybean and corn production in the midwestern United States. An invasive species, the brown marmorated stink bug, Halyomorpha halys (Sta° l), is spreading through the region. However, little is known about the complex of stink bug species associated with corn and soybean in the midwestern United States. In this region, particularly in the more northern states, stink bugs have historically caused only infrequent impacts to these crops. To prepare growers and agricultural professionals to contend with this new threat, we provide a review of stink bugs associated with soybean and …

Quantifying Ephemeral Gully Erosion With Photogrammetry Surveying, C. B. Weerasekara, A. Y. Shechukov

Conservation and Survey Division: Faculty and Staff Publications

No abstract provided.

A Survey On Automated Food Monitoring And Dietary Management Systems, Vieira Bruno, Silva Resende, Juan Cui

School of Computing: Faculty Publications

Healthy diet with balanced nutrition is key to the prevention of life-threatening diseases such as obesity, cardiovascular disease, and cancer. Recent advances in smartphone and wearable sensor technologies have led to a proliferation of food monitoring applications based on automated food image processing and eating episode detection, with the goal to conquer drawbacks of the traditional manual food journaling that is time consuming, inaccurate, underreporting, and low adherent. In order to provide users feedback with nutritional information accompanied by insightful dietary advice, various techniques in light of the key computational learning principles have been explored. This survey presents a variety …

A Translation Of The Malia Altar Stone, Peter Z. Revesz

School of Computing: Faculty Publications

This paper presents a translation of the Malia Altar Stone inscription (CHIC 328), which is one of the longest known Cretan Hieroglyph inscriptions. The translation uses a synoptic transliteration to several scripts that are related to the Malia Altar Stone script. The synoptic transliteration strengthens the derived phonetic values and allows avoiding certain errors that would result from reliance on just a single transliteration. The synoptic transliteration is similar to a multiple alignment of related genomes in bioinformatics in order to derive the genetic sequence of a putative common ancestor of all the aligned genomes.

Towards Internet Of Underground Things In Smart Lighting: A Statistical Model Of Wireless Underground Channel, Abdul Salam, Mehmet C. Vuran, Suat Irmak

School of Computing: Conference and Workshop Papers

The Internet of Underground Things (IOUT) has many applications in the area of smart lighting. IOUT enables communications in smart lighting through underground (UG) and aboveground (AG) communication channels. In IOUT communications, an in-depth analysis of the wireless underground channel is important to design smart lighting solutions. In this paper, based on the empirical and the statistical analysis, a statistical channel model for the UG channel has been developed. The parameters for the statistical tapped-delay-line model are extracted from the measured power delay profiles (PDP). The PDP of the UG channel is represented by the exponential decay of the lateral, …

Biosimp: Using Software Testing Techniques For Sampling And Inference In Biological Organisms, Mikaela Cashman, Jennie L. Catlett, Myra B. Cohen, Nicole R. Buan, Zahmeeth Sakkaff, Massimiliano Pierobon, Christine A. Kelley

School of Computing: Conference and Workshop Papers

Years of research in software engineering have given us novel ways to reason about, test, and predict the behavior of complex software systems that contain hundreds of thousands of lines of code. Many of these techniques have been inspired by nature such as genetic algorithms, swarm intelligence, and ant colony optimization. In this paper we reverse the direction and present BioSIMP, a process that models and predicts the behavior of biological organisms to aid in the emerging field of systems biology. It utilizes techniques from testing and modeling of highly-configurable software systems. Using both experimental and simulation data we show …

Satisfaction Among Early And Mid-Career Dentists In A Metropolitan Dental Hospital In China, Xiaoxi Cui, David G. Dunning, Na An

College of Dentistry: Faculty Publications

A growing body of research has examined career satisfaction among dentists using a standardized instrument, dentist satisfaction survey (DSS). This project examined career satisfaction of early to mid-career dentists in China, a population whose career satisfaction, heretofore, has not been studied. This is an especially critical time to examine career satisfaction because of health care reform measures being implemented in China. A culturally sensitive Chinese-language version of the DSS (CDSS) was developed and electronically administered to 367 early and mid-career dentists in a tertiary dental hospital in Beijing, China. One hundred and seventy respondents completed the survey. The average total …

In-Situ Terahertz Optical Hall Effect Measurements Of Ambient Effects On Free Charge Carrier Properties Of Epitaxial Graphene, Sean Knight, Tino Hofmann, Chamseddine Bouhafs, Nerijus Armakavicius, Philipp Kühne, Vallery Stanishev, Ivan G. Ivanov, Rositsa Yakimova, Shawn Wimer, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Unraveling the doping-related charge carrier scattering mechanisms in two-dimensional materials such as graphene is vital for limiting parasitic electrical conductivity losses in future electronic applications. While electric field doping is well understood, assessment of mobility and density as a function of chemical doping remained a challenge thus far. In this work, we investigate the effects of cyclically exposing epitaxial graphene to controlled inert gases and ambient humidity conditions, while measuring the Lorentz force-induced birefringence in graphene at Terahertz frequencies in magnetic fields. This technique, previously identified as the optical analogue of the electrical Hall effect, permits here measurement of charge …

Rainbow Peacock Spiders Inspire Miniature Superiridescent Optics, Bor-Kai Hsiung, Radwanul Hasan Siddique, Doekele G. Stavenga, Jürgen C. Otto, Michael C. Allen, Ying Liu, Yongfeng Lu, Dimitri D. Deheyn, Matthew D. Shawkey, Todd A. Blackledge

Department of Electrical and Computer Engineering: Faculty Publications

Colour produced by wavelength-dependent light scattering is a key component of visual communication in nature and acts particularly strongly in visual signalling by structurallycoloured animals during courtship. Two miniature peacock spiders (Maratus robinsoni and M. chrysomelas) court females using tiny structured scales (~ 40 × 10 μm²) that reflect the full visual spectrum. Using TEM and optical modelling, we show that the spiders’ scales have 2D nanogratings on microscale 3D convex surfaces with at least twice the resolving power of a conventional 2D diffraction grating of the same period. Whereas the long optical path lengths required …

Controllable Plasmonic Nanostructures Induced By Dual-Wavelength Femtosecond Laser Irradiation, Weina Han, Lan Jiang, Xiaowei Li, Qingsong Wang, Shaojun Wang, Jie Hu, Yongfeng Lu

Department of Electrical and Computer Engineering: Faculty Publications

We demonstrated an abnormal double-peak (annular shaped) energy deposition through dualwavelength synthesis of the fundamental frequency (ω) and the second-harmonic frequency (2ω) of a femtosecond (fs) Ti:sapphire laser. The annular shaped distribution of the dual-wavelength fs laser was confirmed through real beam shape detection. This uniquely simple and flexible technique enables the formation of functional plasmonic nanostructures. We applied this double-peak fs-laser-induced dewetting effect to the controlled fabrication and precise deposition of Au nanostructures, by using a simple, lithography-free, and single-step process. In this process, the double-peak energy-shaped fs laser pulse induces surface patterning of a thin film followed by …

Preparation Of Monolayer Mos2 Quantum Dots Using Temporally Shaped Femtosecond Laser Ablation Of Bulk Mos2 Targets In Water, Bo Li, Lan Jiang, Xin Li, Peng Ran, Pei Zuo, Andong Wang, Liangti Qu, Yang Zhao, Zhihua Cheng, Yongfeng Lu

Department of Electrical and Computer Engineering: Faculty Publications

Zero-dimensional MoS₂ quantum dots (QDs) possess distinct physical and chemical properties, which have garnered them considerable attention and facilitates their use in a broad range of applications. In this study, we prepared monolayer MoS₂ QDs using temporally shaped femtosecond laser ablation of bulk MoS₂ targets in water. The morphology, crystal structures, chemical, and optical properties of the MoS₂ QDs were characterized by transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, UV–vis absorption spectra, and photoluminescence spectra. The analysis results show that highly pure, uniform, and monolayer MoS₂ QDs can be successfully prepared. Moreover, …

Flatland Plasmonics And Nanophotonics Based On Graphene And Beyond, Pai-Yen Chen, Christos Argyropoulos, Mohamed Farhat, J. Sebastian Gomez-Diaz

Department of Electrical and Computer Engineering: Faculty Publications

In this paper, we review and discuss how the recently discovered two-dimensional (2D) Dirac materials, particularly graphene, may be utilized as new efficient platforms for excitations of propagating and localized surface plasmon polaritons (SPPs) in the terahertz (THz) and mid-infrared (MIR) regions. The surface plasmon modes supported by the metallic 2D materials exhibit tunable plasmon resonances that are essential, yet missing, ingredients needed for THz and MIR photonic and optoelectronic devices. We describe how the atomically thin graphene monolayer and metamaterial structures based on it may tailor and control the spectral, spatial, and temporal properties of electromagnetic radiation. In the …

Higher C-Reactive Protein Levels Predict Postoperative Delirium In Older Patients Undergoing Major Elective Surgery: A Longitudinal Nested Case-Control Study, Simon T. Dillon, Sarinnapha M. Vasunilashorn, Long Ngo, Hasan H. Otu, Sharon K. Inouye, Richard N. Jones, David C. Alsop, George A. Kuchel, Eran D. Metzger, Steven E. Arnold, Edward R. Marcantonio, Towia A. Libermann

Department of Electrical and Computer Engineering: Faculty Publications

Background—Delirium is a common, morbid, and costly postoperative complication.. We aimed to identify blood-based postoperative delirium markers in a nested case control study of older surgical patients using a proteomics approach followed by enzyme-linked immunosorbent assay (ELISA) validation.

Methods and Materials—The Successful Aging after Elective Surgery Study enrolled dementia-free adults age ≥70 undergoing major scheduled non-cardiac surgery (N=566; 24% delirium). Plasma was collected at 4 timepoints: preoperatively (PREOP), post-anesthesia care unit (PACU), postoperative day 2 (POD2) and 1 month follow-up (PO1MO). Matched pairs were selected for the independent discovery (39 pairs) and replication cohorts (36 pairs), which were …

Band-To-Band Transitions, Selection Rules, Effective Mass, And Excitonic Contributions In Monoclinic Β-Ga2o3, Alyssa Mock, Rafal Korlacki, Chad Briley, Vanya Darakchieva, Bo Monemar, Yoshinao Kumagai, Ken Goto, Masataka Higashiwaki, Mathias Schubert

Department of Electrical and Computer Engineering: Faculty Publications

We employ an eigenpolarization model including the description of direction dependent excitonic effects for rendering critical point structures within the dielectric function tensor of monoclinic β-Ga₂O₃ yielding a comprehensive analysis of generalized ellipsometry data obtained from 0.75–9 eV. The eigenpolarization model permits complete description of the dielectric response. We obtain, for single-electron and excitonic band-to-band transitions, anisotropic critical point model parameters including their polarization vectors within the monoclinic lattice. We compare our experimental analysis with results from density functional theory calculations performed using the Gaussian-attenuation-Perdew-Burke-Ernzerhof hybrid density functional. We present and discuss the order of the …

Resistant Starch Can Improve Insulin Sensitivity Independently Of The Gut Microbiota, Laure B. Bindels, Rafael R. Segura Munoz, João Carlos Gomes-Neto, Valentin Mutemberezi, Inés Martínez, Nuria Salazar, Elizabeth A. Cody, Maria I. Quintero-Villegas, Hatem Kittana, Clara G De Los Reyes-Gavilán, Robert J. Schmaltz, Giulio G. Muccioli, Jens Walter, Amanda E. Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Background: Obesity-related diseases, including type 2 diabetes and cardiovascular disease, have reached epidemic proportions in industrialized nations, and dietary interventions for their prevention are therefore important. Resistant starches (RS) improve insulin sensitivity in clinical trials, but the mechanisms underlying this health benefit remain poorly understood. Because RS fermentation by the gut microbiota results in the formation of physiologically active metabolites, we chose to specifically determine the role of the gut microbiota in mediating the metabolic benefits of RS. To achieve this goal, we determined the effects of RS when added to a Western diet on host metabolism in mice with …

Negative Binomial Mixed Models For Analyzing Microbiome Count Data, Xinyan Zhang, Himel Mallick, Zaixiang Tang, Lei Zhang, Xiangqin Cui, Andrew K. Benson, Nengjun Yi

Department of Food Science and Technology: Faculty Publications

Background: Recent advances in next-generation sequencing (NGS) technology enable researchers to collect a large volume of metagenomic sequencing data. These data provide valuable resources for investigating interactions between the microbiome and host environmental/clinical factors. In addition to the well-known properties of microbiome count measurements, for example, varied total sequence reads across samples, over-dispersion and zero-inflation, microbiome studies usually collect samples with hierarchical structures, which introduce correlation among the samples and thus further complicate the analysis and interpretation of microbiome count data.

Results: In this article, we propose negative binomial mixed models (NBMMs) for detecting the association between the microbiome and …

Profile Of Polyphenol Compounds Of Five Muscadine Grapes Cultivated In The United States And In Newly Adapted Locations In China, Zheng Wei, Jianming Luo, Yu Huang, Wenfeng Guo, Yali Zhang, Huan Guan, Changmou Xu, Jiang Lu

Department of Food Science and Technology: Faculty Publications

Polyphenol compositions and concentrations in skins and seeds of five muscadine grapes (cv. “Noble”, “Alachua”, “Carlos”, “Fry”, and “Granny Val”) cultivated in the United States (Tallahassee-Florida, TA-FL) and South China (Nanning-Guangxi, NN-GX and Pu’er-Yunnan, PE-YN) were investigated, using ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC Triple TOF MS/MS). Fourteen ellagitannins were newly identified in these muscadine grapes. The grapes grown in NN-GX accumulated higher levels of ellagic acid, methyl brevifolin carboxylate, and ellagic acid glucoside in skins, and penta-O-galloyl-glucose in seeds. In PE-YN, more flavonols were detected in skins, and higher contents of flavan-3-ols, ellagic acid, …

A Critical Assessment Of The “Sterile Womb” And “In Utero Colonization” Hypotheses: Implications For Research On The Pioneer Infant Microbiome, Maria Elisa Perez-Muñoz, Marie-Claire Arrieta, Amanda E. Ramer-Tait, Jens Walter

Department of Food Science and Technology: Faculty Publications

After more than a century of active research, the notion that the human fetal environment is sterile and that the neonate’s microbiome is acquired during and after birth was an accepted dogma. However, recent studies using molecular techniques suggest bacterial communities in the placenta, amniotic fluid, and meconium from healthy pregnancies. These findings have led many scientists to challenge the “sterile womb paradigm” and propose that microbiome acquisition instead begins in utero, an idea that would fundamentally change our understanding of gut microbiota acquisition and its role in human development. In this review, we provide a critical assessment of the …

The International Scientific Association For Probiotics And Prebiotics (Isapp) Consensus Statement On The Definition And Scope Of Prebiotics, Glenn R. Gibson, Robert W. Hutkins, Mary Ellen Sanders, Susan L. Prescott, Raylene A. Reimer, Seppo J. Salminen, Karen Scott, Catherine Stanton, Kelly S. Swanson, Patrice D. Cani, Kristin Verbeke, Gregor Reid

Department of Food Science and Technology: Faculty Publications

In December 2016, a panel of experts in microbiology, nutrition and clinical research was convened by the International Scientific Association for Probiotics and Prebiotics to review the definition and scope of prebiotics. Consistent with the original embodiment of prebiotics, but aware of the latest scientific and clinical developments, the panel updated the definition of a prebiotic: a substrate that is selectively utilized by host microorganisms conferring a health benefit. This definition expands the concept of prebiotics to possibly include non-carbohydrate substances, applications to body sites other than the gastrointestinal tract, and diverse categories other than food. The requirement for selective …

Subgingival Microbiome Colonization And Cytokine Production During Early Dental Implant Healing, Jeffrey B. Payne, Paul G. Johnson, Car Reen Kok, João Carlos Gomes-Neto, Amanda Ramer-Tait, Marian J. Schmid, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

Little is known about longitudinal development of the peri-implant subgingival microbiome and cytokine production as a new sulcus forms after dental implant placement. Therefore, the purpose of this observational study was to evaluate simultaneous longitudinal changes in the oral microbiome and cytokine production in the developing peri-implant sulcus compared to control natural teeth. Four and 12 weeks after implant placement and abutment connection, a dental implant and a natural tooth were sampled in 25 patients for subgingival plaque and gingival crevicular fluid (GCF [around teeth] and peri-implant crevicular fluid [PICF] around implants). DNA from plaque samples was extracted and sequenced …