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2017

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Peucedanum Guvenianum (Apiaceae), A New Species From West Anatolia, Turkey, Hasan Yildirim, Hayri̇ Duman Jan 2017

Peucedanum Guvenianum (Apiaceae), A New Species From West Anatolia, Turkey, Hasan Yildirim, Hayri̇ Duman

Turkish Journal of Botany

Peucedanum guvenianum Yıldırım & H.Duman is described as a species new to science. It is endemic to the West Anatolia region of Turkey. It is known from a single locality in İzmir Province. P. guvenianum shows similarities to P. longifolium, P. ruthenicum, and P. vourinense. Diagnostic morphological characters are discussed and compared with those of closely related taxa. It is easily distinguished from related species especially by its stem 130-220 cm tall, distinctly striate, densely branched from below the middle to upper part; basal leaves 28-40 cm long and 20-70 cm wide; bracts linear-lanceolate and erect; petals emarginate at apex; …

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Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler Jan 2017

Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler

Turkish Journal of Biology

Leaf rolling (LR), particularly in crops, is a dehydration avoidance mechanism that results from water loss in plants under drought conditions. Two maize cultivars (Zea mays L.) differing in their tolerance to drought (cultivar Side was selected as stress-tolerant and cultivar Karaçay was selected as stress-sensitive) under PEG-induced drought stress were used in this study to understand the effects of polyamines (PAs) on LR response and the role of the antioxidant system in the delayed LR process. Aerial parts of the seedlings were excised prior to the experiment and then submerged in a Hoagland nutrient solution with or without 0.1 …

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The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada Jan 2017

The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada

Turkish Journal of Biology

The aim of this study was to increase laccase production in Trametes versicolor ATCC 200801 pellets by using various inducers and their combinations under repeated-batch conditions. Because copper is an effective inducer for laccase production, the effect of Cu on laccase production in this strain was tested first. The optimal Cu concentration for the highest laccase production was 0.5 mM. Following this preliminary study, the effect of other inducers [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), syringaldazine, guaiacol, and 2,5-xylidine] on laccase production was determined. Copper was determined to be the most efficient inducer among the inducers used. Therefore, the synergistic effect of each …

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Contents Of Phenolics And Carotenoids In Tomato Grown Underpolytunnels With Different Uv-Transmission Rates, Bojana Zivanovic, Marija Vidovic, Sonja Milic Komic, Ljubinko Jovanovic, Predrag Kolarz, Filis Morina, Sonja Veljovic Jovanovic Jan 2017

Contents Of Phenolics And Carotenoids In Tomato Grown Underpolytunnels With Different Uv-Transmission Rates, Bojana Zivanovic, Marija Vidovic, Sonja Milic Komic, Ljubinko Jovanovic, Predrag Kolarz, Filis Morina, Sonja Veljovic Jovanovic

Turkish Journal of Agriculture and Forestry

Tomato (Solanum lycopersicum L.) is among the economically most important vegetables in Europe, valued for its bioactive properties due to significant contents of vitamins, carotenoids, and phenolic compounds. In this study, the tomato cultivar Big Beef F1 was grown in the open field (OF) and under polytunnels in central Serbia during 3 years. Polytunnels were covered with two foils (both with 57% reduced photosynthetic active radiation, PAR) differing in UV-A and UV-B transmittance. The aim of our work was to determine the influence of light conditions on accumulation of phytonutrients (carotenoids and phenolics) in the peel and flesh of ripe …

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Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone Jan 2017

Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone

Turkish Journal of Biology

n thermally damaged archaeological bones the quantity, quality, and amplifiability of DNA are very much reliant on both the extent of heating and the environmental conditions of the burial context. In this study we tested the possibility of extracting and amplifying ancient DNA from human bone remains of Herculaneum victims of the 79 AD eruption of Vesuvius using a combination of histochemical and molecular methods. Long bone samples with variable degrees of chromatic and morphological alterations consistent with exposure to temperatures of about 300 °C were taken from four specimens. Using histochemical stains, bone cryostat sections from three individuals revealed …

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Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu Jan 2017

Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu

Turkish Journal of Biology

Exogenous proline (PRO) at low concentrations can enhance drought stress tolerance in different application modes such as application to rooting medium, foliar spray, and seed soaking. However, there is no information about which application mode is more effective for increasing the drought tolerance. Comparative effects of 1, 10, and 20 mM PRO applications through three application modes to hydroponically grown seedlings were examined under short-term drought stress in maize seedlings. Effects on leaf water potential, membrane damage, chlorophyll content, proline level, and gas exchange parameters such as net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (gs), and substomatal CO2 …

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Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z Jan 2017

Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z

Turkish Journal of Biology

Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. …

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Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça Jan 2017

Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça

Turkish Journal of Biology

Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in …

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Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu Jan 2017

Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu

Turkish Journal of Biology

The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the …

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Efficacy Of Trichogramma Evanescens And Bacillus Thuringiensis Var. Kurstaki In Control Of Cydia Pomonella (L.) In Turkey, Sevcan Öztemi̇z, Ali̇ Küden, Serpi̇l Nas, Işilay Lavkor Jan 2017

Efficacy Of Trichogramma Evanescens And Bacillus Thuringiensis Var. Kurstaki In Control Of Cydia Pomonella (L.) In Turkey, Sevcan Öztemi̇z, Ali̇ Küden, Serpi̇l Nas, Işilay Lavkor

Turkish Journal of Agriculture and Forestry

This study was conducted to evaluate the effectiveness of Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae) release with as well as without Bacillus thuringiensis var. kurstaki (Berliner) in biological control of Cydia pomonella (L.) (Lepidoptera: Tortricidae) in the Galaxy Gala apple variety grafted onto M9 rootstock in 2016. Four treatments were analyzed: releasing T. evanescens alone (TE), applying B. thuringiensis var. kurstaki (BT) alone, applying both (TE + BT), and a control (C) without any application at all. The experiment design entailed randomized blocks with four replicates. In each generation of the egg stage, 100,000 parasitoids per hectare were released twice (1440 …

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Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal Jan 2017

Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal

Turkish Journal of Biology

Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of …

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Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li Jan 2017

Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li

Turkish Journal of Biology

Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …

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Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary Jan 2017

Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary

Turkish Journal of Biology

The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth …

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Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan Jan 2017

Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan

Turkish Journal of Biology

Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …

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Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu Jan 2017

Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu

Turkish Journal of Biology

Human carbonic anhydrase II (hCA II) enzyme was firstly expressed using a pET-SUMO expression vector in Escherichia coli and the recombinant enzyme was purified using nickel (Ni2+) affinity chromatography. The substitutions of Trp 209 with four amino acid (Val, Leu, Ile, and Pro) in the hydrophobic pocket of hCA II were conducted using site-directed mutagenesis. The p-nitrophenyl esterase activity of hCA II variants correlates with the hydrophobicity and size of residue, suggesting that the hydrophobic character of this residue is important for catalysis. The Trp 209 was forecast as an important residue and was exposed to computational mutagenesis. This forecast …

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Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic Jan 2017

Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic

Turkish Journal of Biology

Galectins (gals) are ß-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin …

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Natural Dissemination Of Hypovirulent Cryphonectria Parasiticastrain Used For Biological Control Of Chestnut Blight, Naci̇ye Mükerrem Çeli̇ker, Cevdet Kaplan, Emi̇n Ersi̇n Onoğur, Barbaros Çeti̇nel, Di̇lek Poyraz, Ayşe Uysal Jan 2017

Natural Dissemination Of Hypovirulent Cryphonectria Parasiticastrain Used For Biological Control Of Chestnut Blight, Naci̇ye Mükerrem Çeli̇ker, Cevdet Kaplan, Emi̇n Ersi̇n Onoğur, Barbaros Çeti̇nel, Di̇lek Poyraz, Ayşe Uysal

Turkish Journal of Agriculture and Forestry

Chestnut blight, caused by Cryphonectria parasitica Murr. Barr, is the most serious disease of chestnut trees in Turkey on which several studies related to biological control were conducted. The aim of this study was to determine the cause of the occurrence of healing cankers on untreated chestnut trees and the role of carriers in the spread of hypovirulence by laboratory tests, and to discover the effect of some environmental factors (temperature and relative humidity) on the natural spreading of hypovirulence. The study was carried out in a chestnut grove where biological control studies of the disease had been performed in …

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In Vitro Production Protocol Of Vaccinium Uliginosum L. (Bog Bilberry)Growing In The Turkish Flora, Mustafa Cüce, Atalay Sökmen Jan 2017

In Vitro Production Protocol Of Vaccinium Uliginosum L. (Bog Bilberry)Growing In The Turkish Flora, Mustafa Cüce, Atalay Sökmen

Turkish Journal of Agriculture and Forestry

This novel approach was designed to increase the production capacity of Vaccinium uliginosum L. via direct organogenesis. Lateral buds containing one or two leaves were initially cultured in McCown woody plant medium (WPM), Anderson's rhododendron medium, and Murashige and Skoog basal media, each supplemented with zeatin/indole-3-butyric acid (IBA) and zeatin/naphthalene acetic acid (NAA) (1.0/0.1 mg L-1). WPM containing the zeatin/IBA combination was the most effective as the basal medium. Various plant growth regulators (PGRs) were then investigated for the best shoot multiplication, and zeatin was found to be most favorable PGR in all cases. The highest shoot length and shoot …

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Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural Jan 2017

Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural

Turkish Journal of Biology

In the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ΔΔCT method. At the last stage of the study, we …

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Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem Jan 2017

Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem

Turkish Journal of Biology

Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology. Since most enterohemorrhagic infections result from water and food contaminations of Escherichia coli O157:H7, selective and rapid detection of this organism in environmental and biological complexes is necessary. In this study, optimized parameters of antibody (Ab)-based LFA for rapid detection of pathogenic E. coli O157:H7 are described. GNPs were used as visualizing agents. The measuring parameters include the Ab concentration …

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Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon Jan 2017

Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon

Turkish Journal of Biology

The characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. The expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 …

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Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz Jan 2017

Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz

Turkish Journal of Biology

For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …

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Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin Jan 2017

Influence Of Vermicompost Application On Soil Consistency Limits And Soil Compactibility, Serdar Sari, Ekrem Lütfi̇ Aksakal, İlker Angin

Turkish Journal of Agriculture and Forestry

Soil organic matter (SOM) is a composite indicator of soil quality. Recycling of organic wastes is one of the environmentally compatible and economically viable options to increase and/or maintain SOM content. Our study was conducted to evaluate the effects of vermicompost (0%, 0.5%, 1%, 2%, and 4% w/w) on the consistency limits and the Proctor compaction test parameters of three texturally different soils (sandy loam, loam, and clay) from similar climatic conditions and cropping management practices. Results showed that vermicompost increased the optimum moisture content (OMC) with an associated decrease in maximum dry soil bulk density (MBD). The increase in …

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Response Of Five Triticale Genotypes To Salt Stress In In Vitro Culture, İsmai̇l Bezi̇rğanoğlu Jan 2017

Response Of Five Triticale Genotypes To Salt Stress In In Vitro Culture, İsmai̇l Bezi̇rğanoğlu

Turkish Journal of Agriculture and Forestry

Salinity is one of the important abiotic stresses that restrict plant development. Triticale is a highly adaptable crop, known for its high-quality grain, yield potential, and resistance to disease. Responses of five cultivated genotypes (Ümran Hanım, Mikham 2002, Melez 2001, Tatlıcak, and Alper Bey) of triticale to salt stress were tested in callus and embryogenic callus formation in this study. Cotyledon explants were selected as explants for callus induction and embryogenic callus formation. The five tested triticale genotypes varied in their callus growth and embryogenic callus formation. Ümran Hanım, Tatlıcak, and Alper Bey were observed for better callus induction; similarly, …

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Diversity And Genetic Analysis Through Dartseq In Common Bean(Phaseolus Vulgaris L.) Germplasm From Turkey, Seda Nemli̇, Tansel Kaygisiz Aşçioğul, Duygu Ateş, Dursun Eşi̇yok, Muhammed Bahatti̇n Tanyolaç Jan 2017

Diversity And Genetic Analysis Through Dartseq In Common Bean(Phaseolus Vulgaris L.) Germplasm From Turkey, Seda Nemli̇, Tansel Kaygisiz Aşçioğul, Duygu Ateş, Dursun Eşi̇yok, Muhammed Bahatti̇n Tanyolaç

Turkish Journal of Agriculture and Forestry

The common bean (Phaseolus vulgaris L.) is the most consumed food legume in the world and is a major source of dietary protein, carbohydrates, and valuable micronutrients, especially in developing countries. Diversity Arrays Technology (DArTseq), based on genome reduction with restriction enzymes, provides a rapid, high-throughput, and cost-effective tool capable of generating thousands of genotyped single nucleotide polymorphisms (SNPs) for a genome-wide analysis of genetic diversity. In this study, we aimed to characterize common bean accessions using SNPs detected by a DArTseq approach. A total of 43,018 SNPs were identified from 173 common bean accessions, including Andean and Mesoamerican genotypes. …

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Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic Jan 2017

Chemical Composition And Morphometric Traits And Yield Ofcarrots Grown In Organic And Integrated Farming Systems, Natasa Sink, Maja Mikulic Petkovsek, Robert Veberic, Nina Kacjan Marsic

Turkish Journal of Agriculture and Forestry

Changes in chemical composition and quality traits of carrot roots (Daucus carota L.) grown in organic (ORG) and integrated (INT) farming systems were investigated during two successive years. Three carrot cultivars (Fanal, Rodelika, and Rolanka) were included. Determinations of sugars, organic acids, α- and ß-carotene, and vitamin C were performed with an HPLC system, and a liquid chromatography-mass spectrometry (LC-MS) system was used for the identification and quantification of phenolics. A higher yield of carrot in the ORG system was observed (42-44 t ha-1) as compared with the INT system (37-40 t ha-1). The impact of farming system on morphometric …

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Antioxidant Capacity Of Oilseed Rape (Brassica Napus) In Different Soil Types, Dusica Jovicic, Jovica Vasin, Zorica Nikolic, Gordana Petrovic, Gordana Tamindzic, Maja Ignjatov, Dragana Milosevic Jan 2017

Antioxidant Capacity Of Oilseed Rape (Brassica Napus) In Different Soil Types, Dusica Jovicic, Jovica Vasin, Zorica Nikolic, Gordana Petrovic, Gordana Tamindzic, Maja Ignjatov, Dragana Milosevic

Turkish Journal of Agriculture and Forestry

This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on …

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The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k Jan 2017

The Effects Of Different Plant Densities And Silage Corn Varieties On Silage Yield And Some Yield Parameters In No-Till Seeding, Mehmet Zahi̇d Malasli, Ali̇ Khatami̇, Ahmet Çeli̇k

Turkish Journal of Agriculture and Forestry

In this study, the possibility of growing silage corn, which is used as an important nutrient in livestock and whose production is increasing in recent years, by no-till seeding method under Erzurum conditions was investigated. For this purpose, three silage corn varieties (DKC-5783, Prestige, and ADA-9510) suitable for Erzurum's climatic conditions in terms of vegetation period were selected from a large number of silage corn seeds widely grown throughout Turkey and their development conditions under no-till seeding conditions were investigated. A precision vacuum no-till seeder with double disk type furrow opener was used for silage corn seeding. Based on three …

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Aftermath Of The Human Genome Project: An Era Of Struggle And Discovery, Zainab Hamdoun, Hashimul Ehsan Jan 2017

Aftermath Of The Human Genome Project: An Era Of Struggle And Discovery, Zainab Hamdoun, Hashimul Ehsan

Turkish Journal of Biology

The collaboration between the Human Genome Project and the Sanger Institute, spurred on by their contemporary- Celera Genomics, in the race to sequence the human genome, opened the door to a generation of research concerned with the study of the nucleic manual. Following this monumental achievement, the genomic era was born, heralding the development of gene therapy, personalized medicine, pharmacogenomics, and CRISPR/Cas9 gene-editing systems. These 'omic' disciplines offer a wide range of applications in the treatment of disease and the betterment of the quality of human life. Their notability, however, manifests in the unique mix of sociological and scientific discourse …

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Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le Jan 2017

Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le

Turkish Journal of Biology

Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the …

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