Identification Of Expression And Function Of The Glucagon-Like Peptide-1 Receptor In Gastrointestinal Smooth Muscle, Alexander T. May

Theses and Dissertations

In response to ingestion of nutrients, enteroendocrine L cells secrete the incretin hormone, glucagon-like peptide-1 (GLP-1), to enhance glucose-dependent insulin release. Therapies related to GLP-1 are approved for type 2 diabetes. The GLP-1 receptor (GLP-1R) is expressed in cells of the gastrointestinal tract and elsewhere. In pancreatic beta cells, GLP-1R are coupled to the Gs/cAMP/PKA pathway. The expression and function of GLP-1R in gastrointestinal smooth muscle are not known. Aim. To test the hypothesis that GLP-1 regulates smooth muscle function by acting on GLP-1R expressed on smooth muscle. Methods. Smooth muscle cells (SMC) were isolated and cultured. Expression of GLP-1R …

Novel Role Of The Nociceptin System As A Regulator Of Glutamate Transporter Expression In Developing Astrocytes, Logan Meyer

Theses and Dissertations

Our previous results showed that oligodendrocyte development is regulated by both nociceptin and its G-protein coupled receptor, the nociceptin/orphanin FQ receptor (NOPR). The present in vitro and in vivo findings show that nociceptin plays a crucial conserved role in both human and rodent brain astrocytes, regulating the levels of the glutamate/aspartate transporter GLAST/EAAT1. This nociceptin-mediated response takes place during a critical developmental window that coincides with astrocyte maturation and synapse formation. GLAST/EAAT1 upregulation by nociceptin is mediated by NOPR and the downstream participation of a complex signaling cascade that involves the interaction of several kinase systems, including PI-3K/AKT, mTOR and …

The Role Of Klf1 In Regulating Γ-Globin Gene Repressors, Anna P. Kovilakath

Theses and Dissertations

Sickle cell disease and β-thalassemia affect millions of people worldwide. γ-globin is the fetal counterpart to the adult β-globin. Research has shown that affected patients with higher than normal γ-globin show less severe symptoms. Therefore, reversing or preventing the hemoglobin switch from γ- to β- globin is a promising avenue of research for treating these diseases. KLF1 is an erythroid transcription factor involved in hemoglobin switching. Herein, we show that KLF1 directly regulates the γ-globin repressor gene LRF in both the mouse and human systems. KLF1 may also directly activate γ-globin expression by binding the promoter. In human HUDEP-2 cells, …

Nanomedicine Drug Delivery Across Mucous Membranes, Michael G. Lancina Iii

Theses and Dissertations

NANOMEDECINE DRUG DELIVERY ACROSS MUCOUS MEMBRANES

By Michael G. Lancina III

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.

Virginia Commonwealth Univeristy, 2017.

Major Director: Dr. Hu Yang, Associate Professor, Chemical and Life Science Engineering

Control over the distribution of therapeutic compounds is a complex and somewhat overlooked field of pharmaceutical research. When swallowing a pill or receiving an injection, it is commonly assumed that drug will spread throughout the body in a more or less uniform concentration and find its way to wherever it is needed. In …

Effects Of Cumulative Risk On Asthma Outcomes In Urban Children And Adolescents, Samantha A. Miadich

Theses and Dissertations

Pediatric asthma disproportionately affects racial/ethnic minority children and children living in low-income, urban areas. Many families living in low-income, urban areas experience a number of stressors that can place children/adolescents at risk for worse asthma outcomes. This study examined the impact of a cumulative risk model of stressors (e.g., ED visits, quick-relief medication use, lung function, asthma control, QOL) in urban children (7-12 years) with persistent asthma. This study further aimed to examine both the original cumulative risk model and an adolescent-specific cumulative risk model as predictors of asthma outcomes in a sample of 60 adolescents (13-17 years). Asthma-related caregiver …

N-Alkyl 4-Methylamphetamine Enantiomers And The Implication For Potential Modulation Of Abuse Liability And Enhancement Of Psychoactive Drug Targeting., Ramsey Sitta

Theses and Dissertations

Drugs of abuse have a long history in humanity. Currently however, a subject of great interest is the phenylalkylamine family of drugs. Not only is the abuse liability of interest but also the potential therapeutic expansion of the capabilities of this family of drugs by utilizing the unique stereospecific effects of the newly discovered hybrid compounds. Based upon prior data of N-Alkyl 4-MA the enantiomers of N-Methyl, N-Ethyl, and N-Propyl were analyzed in hDAT, hNET, and hSERT. It was found that there was a negative correlation between chain length and potency and dopaminergic component. In agreement with the currently established …

Discovery Of Mor Selective, Reversible Opioid Antagonist For Potential Use In Treatment Of Drug Dependence, Abdulmajeed Jali

Theses and Dissertations

Abstract

DISCOVERY OF MOR SELECTIVE, REVERSIBLE OPIOID ANTAGONIST FOR POTENTIAL USE IN TREATMENT OF DRUG DEPENDENCE

Abdulmajeed M. Jali, M.S.

A thesis submitted in partial fulfillment of the requirements of Master of Science at Virginia Commonwealth University.

Virginia Commonwealth University, 2017

Director: Dana E. Selley, Ph. D., Department of Pharmacology and Toxicology

Opioid dependence/addiction is a major public health problem that is associated with multiple health and social costs. Pharmacotherapeutic treatment has been relatively effective, but the risk of relapse after treatment remains high. Naltrexone, an opioid antagonist, is FDA-approved for long-term maintenance therapy to reduce relapse risk. However, naltrexone …

Analyzing The Phenotypic Effect Of Three Candidate Genes Associated With Nonsyndromic Craniosynostosis Using A Zebrafish Model, Megan A. Hept

Theses and Dissertations

In normal cranial suture development, the cranial sutures close at predetermined periods of development to allow the brain the capability to grow in a malleable environment. However, in craniosynostosis, cranial sutures prematurely fuse before birth which can lead to a wide range of developmental issues and complications. Craniosynostosis can be categorized as nonsyndromic which involves the sole fusion of one or more of the cranial sutures, or syndromic in which cranial sutures fuse as well as other abnormalities associated with a genetic disorder. Past research has identified three candidate genes that could be possible disease causing mutations in nonsyndromic sagittal …

Eye Movement Control: An Index For Athleticism, Brittany L. Oshea

Theses and Dissertations

Athletic potential is one of the most complex human traits. An elite athlete is produced from a complex interaction of an innumerable number of traits exhibited by the athlete. However, it’s not clear whether these traits are innate, allowing the athlete to excel, or, alternatively, are a consequence of practice. To be successful, athletes rely heavily on sensory information from the visual and vestibular systems. This study investigated the relationship eye movement control has with innate athleticism by comparing the saccadic and Vestibulo-Ocular Reflex (VOR) responses of former, no longer practicing, elite athletes against their age and gender matched counterparts …

Communication, Empathy, And Trust: Exploring Teachers' Partnerships With The Families Of Their Most Challenging Students, Kimberly W. Mcknight

Theses and Dissertations

The purpose of this embedded mixed methods collective case study was to explore eight kindergarten, first, and second grade teachers’ experiences partnering with families of their students who are at risk for emotional or behavioral disorders (EBDs). The teachers worked in two high-poverty, non-accredited Title-1 schools in an urban city. The study was part of a federally funded intervention called Behavioral, Emotional, and Social Training: Competent Learners Achieving School Success (BEST in CLASS- Elementary; BiC-E; Sutherland et al., 2017), which is targeted for students at risk for the development of EBDs. It uses evidence- based instructional practices to decrease students’ …

Longitudinal Bidirectional Relations Between Subtypes Of Anxiety And Callous-Unemotional Traits In Early- To Mid-Adolescence, Stephanie A. Hitti

Theses and Dissertations

Callous-Unemotional (CU) traits are characterized by limited empathy, lack of guilt or remorse, and callous use of others. They are a risk factor for adult psychopathy, especially when comorbid with conduct problems. Thus, efforts to identify risk factors and consequences of CU traits have been prominent. One construct that may act as both a risk factor for and consequence of CU traits among youth is anxiety. While the most consistent finding is in this literature is a negative relation between CU traits and anxiety, findings have been mixed. The present study examined bidirectional relations between three subtypes of anxiety (i.e. …

The Influence Of Family Composition On Adolescent Problem Behavior: The Moderating Roles Of Gender And Adult Support, Jasmine Coleman

Theses and Dissertations

There is convincing support for the link between family composition and adolescents’ problem behaviors. What is less clear is the extent to which these relations exist for African-American adolescents. Previous studies have demonstrated that this relation varies by gender. However, there is limited evidence to suggest the potential moderating influence of adult support. The purpose of this study was to examine the influence of family composition on adolescents’ physical aggression, delinquency, and substance use. The study comes from secondary analyses of a larger study that evaluated the effectiveness of a violence prevention program. The current study included 1,116 African-American middle …

Integrating Genetics And Neuroimaging To Study Subtypes Of Binge Drinkers, Megan E. Cooke

Theses and Dissertations

Risky alcohol use is a major health concern among college students, with 40.1% reporting binge drinking (5 or more drinks in one occasion) and 14.4% reporting heavy drinking (binge drinking on 5 or more occasions) in the past month. Risky alcohol use is thought to be the result of a complex interplay between genes, biological processes, and other phenotypic characteristics. Understanding this complex relationship is further complicated by known phenotypic heterogeneity in the development of alcohol use. Developmental studies have suggested two pathways to risky alcohol use, characterized by externalizing and internalizing characteristics, respectively. However, the underlying biological processes that …

Podocyte Dedifferentiation And Glomerular Injury Mediated By Lysosome Dysfunction: Role Of Acid Ceramidase, Guangbi Li

Theses and Dissertations

Cell differentiation and senescence in podocytes are attributed to normal autophagy and associated cellular activities. It is possible that derangement of autophagy under different pathological conditions activates or enhances podocyte dedifferentiation leading to glomerular injury and ultimate glomerular disease. To test this hypothesis, we first tested whether autophagic deficiency due to lysosome dysfunction enhances podocyte dedifferentiation and explored the molecular mechanisms by which this lysosome dysfunction trigger or enhance podocyte dedifferentiation. By Western blot and confocal analysis, lysosome inhibition using an inhibitor or siRNA of V-ATPase inhibitor was found to markedly decrease the epithelial markers (P-cadherin and ZO-1) and increase …

Enhanced Energy Storage And Conversion Applications By Porous Carbon And Atomic Layer Deposition, Yomna H. Abdelmoaty

Theses and Dissertations

The design and synthesis of porous materials attracted great attention recently because of their potential use in many fields like clean energy and environmental protection. Herein, we introduced new synthetic approaches for the preparation for porous carbons and organic polymers for selective CO₂ and iodine capture application. Regarding CO₂ capture application, Two new

series of porous carbons (PYDCs) and (TRI-Ps) were synthesized by thermally activating Pyrazole and Triazolo Pyridine monomers respectively using KOH as a catalyst, which leads to porous carbon. PYDCs exhibit high surface area according to Brunauer–Emmett–Tellertheory (SA_BET = 1266–2013 m² g⁻¹), …

Structure-Activity Relationship Studies Of Bupropion And Related 3-Substituted Methcathinone Analogues At Monoamine Transporters, Abdelrahman R. Shalabi

Theses and Dissertations

The khat plant, catha edulis, has been abused for some time in the Middle East and the African horn for its short-term stimulant effects. However, it was not until 1975 when cathinone, β-ketoamphetamine, was identified as the major stimulant component of khat. Structural analogues of cathinone, synthetic cathinones, are new psychoactive substances available on the clandestine market of numerous countries including the USA. Abuse of these new illicit stimulants is a worldwide growing health concern which necessitates the investigation of the pharmacological properties of these new drugs of abuse. The abuse liabilities of these compounds seem to be related …

Pelagic Fish Diversity And Density On And Off Restored Oyster Reef Habitat, Danielle Mcculloch

Theses and Dissertations

The heterogeneity provided by structured habitats is important in supporting diverse and dense fish communities. The biogenic reefs created by the native Eastern Oyster, Crassostrea virginica, were once the dominant structural habitat in Chesapeake Bay, and have since declined to less than 1% of historic estimates. Conflicting results on the effects of oyster reef restoration on pelagic fish assemblages make further investigation necessary. Incorporating multiple sampling strategies may help elucidate oyster reef habitat influence on fish assemblages. This study used multi-panel gillnets, hydroacoustic technology, and day-night sampling to describe pelagic fish assemblages on and off oyster reef habitat in the …

Evaluation Of Filtration And Dna Extraction Methods For Environmental Dna Biodiversity Assessments Across Multiple Trophic Levels, Anni Djurhuus, Jesse Port, Collin Closek, Kevan Yamahara, Ofelia Romero-Maraccini, Kristine Walz, Dawn Goldsmith, Reiko Michisaki, Mya Breitbart, Alexandria Boehm, Francisco Chavez

Marine Science Faculty Publications

Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive seawater sample. Here we compare results of eDNA metabarcoding of multiple trophic levels from individual seawater samples collected from a kelp forest in Monterey Bay, California in order to establish methods for future cross-trophic level eDNA analysis. Triplicate 1 L water samples were filtered using five different 47 mm diameter membrane filters (PVDF, PES, GFF, PCTE, and NC) and DNA was extracted from triplicates of each filter-type using three widely-used …

Spatial Variability Of Spanish Sardine (Sardinella Aurita) Abundance As Related To The Upwelling Cycle Off The Southeastern Caribbean Sea, Digna Rueda-Roa, Jeremy Mendoza, Frank E. Muller-Karger, Juan José Cárdenas, Alina Achury, Yrene Astor

Marine Science Faculty Publications

The Sardinella aurita fishery off northeastern Venezuela, region of seasonal wind-driven coastal-upwelling, accounts for 90% of the Caribbean Sea small pelagic catch. This law-protected artisanal fishery takes place up to ∼10 km offshore. The spatial distribution, number of schools, and biomass of S. aurita were studied using eight hydro-acoustic surveys (1995-1998). The study included the analysis of satellite-derived sea surface temperature and chlorophyll-a. Surveys were grouped by strong, weak, and transitional upwelling seasons. Relationships between these observations were analyzed using Generalized Additive Models. Results show that during the primary upwelling season (January-May) sardines were widely distributed in upwelling plumes that …

Remote Estimation Of Biomass Of Ulva Prolifera Macroalgae In The Yellow Sea, Lianbo Hu, Chuanmin Hu, He Ming-Xia

Marine Science Faculty Publications

Since 2008, macroalgal blooms of Ulva prolifera (also called green tides) occurred every summer in the Yellow Sea (YS), causing environmental and economic problems. A number of studies have used satellite observations to estimate the severity of the blooms through estimating the bloom size and duration. However, a critical bloom parameter, namely biomass, has never been objectively determined due to lack of measurements. In this study, laboratory experiments were conducted to measure U. prolifera biomass (wet weight) per unit area and the corresponding spectral reflectance, through which a robust relationship has been established to link biomass per area to …

The Acceleration Of Dissolved Cobalt's Ecological Stoichiometry Due To Biological Uptake, Remineralization, And Scavenging In The Atlantic Ocean, Mak A. Saito, Abagail E. Noble, Nicholas Hawco, Benjamin S. Twining, Daniel C. Ohnemus, Seth G. John, Phoebe Lam, Tim M. Conway, Rod Johnson, Dawn Moran, Matthew Mcilvin

School of Geosciences Faculty and Staff Publications

The stoichiometry of biological components and their influence on dissolved distributions have long been of interest in the study of the oceans. Cobalt has the smallest oceanic inventory of inorganic micronutrients and hence is particularly vulnerable to influence by internal oceanic processes including euphotic zone uptake, remineralization, and scavenging. Here we observe not only large variations in dCo:P stoichiometry but also the acceleration of those dCo:P ratios in the upper water column in response to several environmental processes. The ecological stoichiometry of total dissolved cobalt (dCo) was examined using data from a US North Atlantic GEOTRACES transect and from a …

Female Art And Artisans In Edith Wharton’S The House Of Mirth, The Custom Of The Country, And “Roman Fever”, Julia B. Welch

College of Graduate Studies: Theses & Dissertations

In early twentieth century old and new New York social circles, the marriage market’s commodification of women acted as the controlling factor for relationships, female power, and personal identity. When considering Wharton’s works for the first-hand viewpoint that she provided of the marriage market, it becomes clear that her interest in art plays heavily into the way women comport themselves within her novels. In order to discuss this relationship in Edith Wharton’s works, I’ve created terms that delineate the various ways female characters respond to the pressures of the marriage market. The best way to analyze Wharton’s women is by …

การใส่กางเกงรัดกล้ามเนื้อต่อการลดการสะสมแลคเตทที่เกิดจากการออกกำลังกายอย่างหนักในโปรแกรมที่ทำให้เกิดการล้าเฉพาะบาสเกตบอลในนักกีฬาบาสเกตบอลชาย, ธวัชชัย พลอยแดง

Chulalongkorn University Theses and Dissertations (Chula ETD)

วัตถุประสงค์ จุดมุ่งหมายของการศึกษานี้เพื่อศึกษาผลของการใส่กางเกงรัดกล้ามเนื้อต่อการลดแลคเตทในเลือดที่เกิดจากการล้าจากการออกกำลังกายแบบเฉพาะของบาสเกตบอลในนักกีฬาบาสเกตบอลชาย วิธีการศึกษา การศึกษาในรูป crossover design ในนักบาสเกตบอลที่มีทักษะสูง จำนวน 19 คน (อายุ 19.95 ± 1.87 ปี ค่าดัชนีมวลกาย 22.45 ± 1.36 กิโลกรัม/ตารางเมตร และค่าความสามารถการใช้ออกซิเจนสูงสุด 47.26 ± 2.83 มิลลิลิตร/กิโลกรัม/นาที) กำหนดรูปแบบให้ใส่กางเกงรัดกล้ามเนื้อ (แรงกดที่ผิวหนัง 16.71 ± 1.35 มิลลิเมตรปรอท) สลับกับกางเกงเลคกิ้ง (3.79 ± 1.47 มิลลิเมตรปรอท) ในลำดับการสุ่มที่เว้นระยะระหว่างโดยเว้นระยะห่างในแต่ละรูปแบบ 5 วัน ทำการวัดระดับความเข้มข้นของแลคเตทจากเลือดที่ปลายนิ้ว สมรรถภาพการกระโดดสูงสุด และ การวัดคลื่นสัญญาณไฟฟ้ากล้ามเนื้อ gastrocnemius ก่อนและหลังการออกกำลังกายแบบเฉพาะของบาสเกตบอล ผลการศึกษา ผลของกางเกงรัดกล้ามเนื้อขณะออกกำลังกายมีค่าแลคเตทในเลือดน้อยกว่ากลุ่มกางเกงเลคกิ้ง อย่างมีนัยสำคัญทางสถิติ (p<0.05) ค่า integrated electromyography และ median frequency ขณะใส่กางเกงรัดกล้ามเนื้อและกางเกงเลคกิ้งช่วงหลังออกกำลังกายเมื่อเปรียบเทียบกับก่อนออกกำลังกายลดลงแตกต่างอย่างมีนัยสำคัญทางสถิติ (p<0.05) ค่าสหสัมพันธ์ (correlation) ระหว่าง การเปลี่ยนแปลง median frequency กับการเปลี่ยนแปลงของระดับแลคเตทในเลือด มีค่าความสัมพันธ์ที่ r = .2 และ r = .47 ในขณะใส่กางเกงรัดกล้ามเนื้อและกางเกงเลคกิ้ง ตามลำดับ สรุปผล ผลการศึกษานี้แสดงให้เห็นว่ากลุ่มกางเกงรัดกล้ามเนื้อสามารถช่วยลดการสะสมของแลคเตทในเลือดขณะออกกำลังกายเฉพาะบาสเกตบอลที่ทำให้เกิดอาการล้าได้ดีกว่าเมื่อเปรียบเทียบกับการใส่กางเกงเลคกิ้ง

การให้ประชาชนเข้าถึงข้อมูลภาครัฐเพื่อการตรวจสอบการทุจริต, วโรดม บุญมั่น

Chulalongkorn University Theses and Dissertations (Chula ETD)

วิทยานิพนธ์ฉบับนี้มุ่งศึกษาถึงสภาพปัญหาของการให้ประชาชนเข้าถึงข้อมูลภาครัฐเพื่อการตรวจสอบการทุจริต และการเปิดเผยข้อมูลภาครัฐแบบเปิด (Open Government Data) โดยศึกษาถึงบทบัญญัติกฎหมาย คำวินิจฉัยของศาลและองค์กรวินิจฉัยข้อพิพาท การบังคับใช้กฎหมายของหน่วยงานและเจ้าหน้าที่ที่เกี่ยวข้อง และนโยบายของรัฐ โดยวิเคราะห์เปรียบเทียบกับบทบัญญัติกฎหมายในระดับสนธิสัญญาระหว่างประเทศ กฎหมายของประเทศสหราชอาณาจักรและกฎหมายระดับสหรัฐของประเทศสหรัฐอเมริกา จากการศึกษาการเปิดเผยข้อมูลภาครัฐเพื่อการตรวจสอบการทุจริตของประเทศไทย พบว่า มีปัญหาดังต่อไปนี้ ประการแรก เนื้อหาของบทบัญญัติกฎหมายที่เกี่ยวข้องยังไม่ครอบคลุมเพียงพอ อาทิ ประเด็นผู้มีสิทธิในการขอให้มีการเปิดเผยข้อมูลข่าวสารของราชการ, ข้อมูลข่าวสารที่กำหนดให้เปิดเผยยังขาดข้อมูลเกี่ยวกับกิจกรรมที่สุ่มเสียงต่อการทุจริต, หลักเกณฑ์การเปิดเผยที่ไม่เป็นเอกภาพ เนื่องจากมีกฎหมายหลายฉบับเกี่ยวข้องกับการเปิดเผยข้อมูลข่าวสาร, รูปแบบและวิธีการเปิดเผยข้อมูลยังไม่ตอบสนองความต้องการของประชาชน ประการที่สอง ปัญหาการบังคับใช้กฎหมายยังขาดกลไกในการเปิดเผยผ่านเว็บไซต์, ขาดกลไกในการตรวจสอบ และมีหลักเกณฑ์การพิจารณาอุทธรณ์ที่ไม่ชัดเจนเพียงพอ นอกจากนี้ องค์กรหลักในการบังคับใช้กฎหมาย คือ คณะกรรมการข้อมูลข่าวสารของราชการยังไม่ประสบความสำเร็จในการควบคุมตรวจสอบ กำกับดูแลให้หน่วยงานของรัฐปฏิบัติตามกฎหมายอย่างเต็มที่ และที่สำคัญ ประการสุดท้าย ยังไม่มีกฎหมายที่ครอบคลุมหลักการเปิดเผยข้อมูลภาครัฐแบบเปิด (Open Government Data) ที่มีมาตรฐานเทียบเท่าระดับสากล จากปัญหาดังกล่าว ผู้วิจัยจึงมีข้อเสนอแนะ ดังต่อไปนี้ ประการแรก ควรมีการปรับปรุงกฎหมายที่มีอยู่ให้ครอบคลุมหลักการเปิดเผยข้อมูลภาครัฐแบบเปิด (Open Government Data) โดยมีการกำหนดรายละเอียดและประเภทของข้อมูลที่เปิดเผย กล่าวคือ ต้องมีการเปิดเผย ข้อมูล เกี่ยวกับการใช้จ่ายเงินงบประมาณ การจัดซื้อจัดจ้างภาครัฐ การอนุญาต การให้สัมปทาน การให้สิทธิ การถือครองกรรมสิทธิ์ การให้ใช้ประโยชน์จากทรัพยากรธรรมชาติ สาธารณสมบัติของแผ่นดิน หรือประโยชน์อื่น ๆ โดยมี รูปแบบ การเปิดเผยข้อมูลที่จะต้องเน้นการเปิดเผยข้อมูลตามเว็บไซต์ที่ค้นหาได้อย่างอิสระ สะดวก รวดเร็ว ดาวน์โหลดได้ไม่จำกัด มีการประมวลผลข้อมูลให้เป็นปัจจุบัน มีความเชื่อมโยงกันระหว่างข้อมูลที่มีการเปิดเผย ครอบคลุมการดำเนินกิจกรรมของรัฐ และสอดคล้องกับหลักการทางบัญชีที่เกี่ยวข้อง สำหรับ การขอข้อมูลเฉพาะราย นั้น ต้องสามารถทำคำขอทางระบบอิเล็กทรอนิกส์ที่ใช้งานได้ง่าย มีหลักเกณฑ์และระยะเวลาพิจารณาคำขอที่ชัดเจน และสามารถเผยแพร่ข้อมูลที่ได้รับต่อไปได้ ประการสุดท้าย การกำกับดูแล จะต้องให้ความเป็นอิสระ อำนาจ วิธีการ หลักเกณฑ์การควบคุมตรวจสอบที่มีประสิทธิภาพแก่องค์กรหรือเจ้าหน้าที่กำกับดูแล และมีบทลงโทษสำหรับกรณีที่มีการไม่เปิดเผยข้อมูลตามที่กฎหมาย หรือคำวินิจฉัยกำหนด

Robust Scale-Invariant Normalization And Similarity Measurement For Time Series Data, Ariyawat Chonbodeechalermroong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Classification is one of the most prevalent tasks in time series mining. Dynamic Time Warping and Longest Common Subsequence are well-known and widely used algorithms to measure similarity between two time series sequences using non-linear alignment. However, these algorithms work best when the time series pair has similar amplitude scaling. Unfortunately, sensor data and most real-world time series data usually contain noise, missing values, outlier, and variability or scaling in both axes, which is not suitable for the widely used Z-normalization. This research introduces the Local Feature Normalization (LFN) and its Local Scaling Feature (LSF), which can be used to …

The Relationship Between Geochemical Parameters And The Communities Of Arsenite-Oxidizing Bacteria In Rayong Groundwater Basin, Phurinat Pipattanajaroenkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

As is one of toxic metalloid commonly found on the earth's surface. In groundwater, arsenite (As3+) and arsenate (As5+) are most predominant As species. Arsenite is more toxic than arsenate. As removal through bioremediation technique can be applied as an alternative method. This study aimed to investigate the community of arsenite-oxidizing bacteria in As-contaminated groundwater and to correlated them to the geochemical parameters. Nineteen groundwater samples were collected from Ban-khai and Muang districts, Rayong province, Thailand. The communities of arsenite-oxidizing bacteria were detected by using PCR-cloning-sequencing techniques, targeting aioA gene. The results showed that the detected arsenite-oxidizing bacteria were associated …

Gold Nanoparticles In Clp Model And Mechanism In Bone Marrows Derived Macrophages Cell For Sepsis Severity Attenuation, Sujittra Taratummarat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sepsis is still a major worldwide- public health problem as a leading cause of the high mortality rate condition. Sepsis is the inflammatory condition in host due to bacterial infections. Sepsis severity depends on the individual's immune responses and the organism virulence factor. The current strategies for sepsis treatment are based, mainly on, symptomatic treatment with antibiotics. However, the adjuvant therapy in sepsis is interesting. Gold nanoparticles are anti-inflammatory drug from the ancient time which is still currently used for the treatment of rheumatoid arthritis. Thus, we hypothesized that gold nanoparticles could be effective in reducing an inflammation in sepsis …

The Effect Of Religious Intervention On Depression And Quality Of Life Among Indonesian Elderly: A Quasi-Experimental Study, Bayu Anggileo Pramesona

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose: This study aimed to investigate the effect of religious intervention on depression and quality of life among Indonesian elderly in nursing homes (NHs). Methods: This was a quasi-experimental study with repeated measures. Sixty elderly residents at three NHs in three districts in Yogyakarta, Indonesia with the Geriatric Depression Scale (GDS) score of 5 to 11 were recruited and purposively assigned to the religious intervention group (combining 36 sessions of listening to Qur'anic recital and three sessions of attending a sermon by a preacher), n=30) and the control group (treatment as usual/TAU, n=30). The primary outcome was depression, measured by …

Microfluidics-Based Single Cell Isolation And Trapping Of Canine Cutaneous Mast Cell Tumor Cells, Dettachai Ketpun

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this research, the primary objective was to develop a microfluidic platform utilized for the size-based single cell sorting of canine cutaneous mast cell tumor (MCT) cells which were categorized into 4 groups; I, II, III and IV, upon their individual sizes ranged from 5-9, 10-14, 15-19, and equal to or over than 20 mm respectively. And the secondary aim was to establish the microfluidic-based entrapping microdevice which had a capacity to ensnare single MCT cells into its triangular microwell array at the ratio of one cell per one microwell. The study consequence consistently indicated the accomplishment of device developments. …

Contemporary Learning Spaces: North Bangkok Demonstration School, Thanyaporn Janma

Chulalongkorn University Theses and Dissertations (Chula ETD)

Schools are places to strengthen learning for youths. At present the content of academic and educational philosophy develops rapidly through technology and society changes but most schools buildings and environments are inconsistent and do not support contemporary learning. As a result, students lack the most effective learning opportunity. However, there are a number of schools that pay attention to their architectural and environmental design to promote students' learning contents and new education philosophy leading to the starting point of this thesis. This thesis studied factors in selecting the learning spaces of Grade 1 - Grade 6 students in order to …