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2017

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Articles 22681 - 22710 of 30195

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Phenotypic And Genomic Survey On Organic Acid Utilization Profile Of Pseudomonas Mendocina Strain S5.2, A Vineyard Soil Isolate, T.M. Chong, J.W. Chen, W.S. See-Too, C.Y. Yu, G.Y. Ang, Y.L. Lim, Wai Fong Yin, C. Grandclément, D. Faure, Y. Dessaux, Kok Gan Chan Jan 2017

Phenotypic And Genomic Survey On Organic Acid Utilization Profile Of Pseudomonas Mendocina Strain S5.2, A Vineyard Soil Isolate, T.M. Chong, J.W. Chen, W.S. See-Too, C.Y. Yu, G.Y. Ang, Y.L. Lim, Wai Fong Yin, C. Grandclément, D. Faure, Y. Dessaux, Kok Gan Chan

Research Publications (2016 to 2020)

Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5.2. Phenotype microarray analysis has demonstrated that this …

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Prevalence Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Infection And The Molecular Characteristics Of Mrsa Bacteraemia Over A Two-Year Period In A Tertiary Teaching Hospital In Malaysia, P.S. Sit, Cindy Shuan Ju Teh, N. Idris, I.C. Sam, S.F.S. Omar, H. Sulaiman, Kwai Lin Thong, Adeeba Kamarulzaman, S. Ponnampalavanar Jan 2017

Prevalence Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Infection And The Molecular Characteristics Of Mrsa Bacteraemia Over A Two-Year Period In A Tertiary Teaching Hospital In Malaysia, P.S. Sit, Cindy Shuan Ju Teh, N. Idris, I.C. Sam, S.F.S. Omar, H. Sulaiman, Kwai Lin Thong, Adeeba Kamarulzaman, S. Ponnampalavanar

Research Publications (2016 to 2020)

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an established pathogen that causes hospital- and community-acquired infections worldwide. The prevalence rate of MRSA infections were reported to be the highest in Asia. As there is limited epidemiological study being done in Malaysia, this study aimed to determine the prevalence of MRSA infection and the molecular characteristics of MRSA bacteraemia. Methods: Two hundred and nine MRSA strains from year 2011 to 2012 were collected from a tertiary teaching hospital in Malaysia. The strains were characterized by antimicrobial susceptibility testing, staphylococcal cassette chromosome mec (SCCmec) typing, detection of Panton-Valentine leukocidin (PVL) gene, multilocus sequence …

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Regions Of Very Low H3k27me3 Partition The Drosophila Genome Into Topological Domains, S. El-Sharnouby, B. Fischer, J.P. Magbanua, B. Umans, R. Flower, S.W. Choo, S. Russell, R. White Jan 2017

Regions Of Very Low H3k27me3 Partition The Drosophila Genome Into Topological Domains, S. El-Sharnouby, B. Fischer, J.P. Magbanua, B. Umans, R. Flower, S.W. Choo, S. Russell, R. White

Research Publications (2016 to 2020)

It is now well established that eukaryote genomes have a common architectural organization into topologically associated domains (TADs) and evidence is accumulating that this organization plays an important role in gene regulation. However, the mechanisms that partition the genome into TADs and the nature of domain boundaries are still poorly understood. We have investigated boundary regions in the Drosophila genome and find that they can be identified as domains of very low H3K27me3. The genome-wide H3K27me3 profile partitions into two states; very low H3K27me3 identifies Depleted (D) domains that contain housekeeping genes and their regulators such as the histone acetyltransferase-containing …

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Reliability Of Panoramic-Radiograph To Determine The Position Of Third-Molar From Inferior-Alveolar-Canal, A.A. Fauzi, M.E. Parker, E. Norval, Phrabhakaran Nambiar Jan 2017

Reliability Of Panoramic-Radiograph To Determine The Position Of Third-Molar From Inferior-Alveolar-Canal, A.A. Fauzi, M.E. Parker, E. Norval, Phrabhakaran Nambiar

Research Publications (2016 to 2020)

Cone-beam-computed-tomography (CBCT) has been useful in providing insights of relevant anatomy prior to surgical procedures, including the assessment of the proximity of impacted mandibular-third-molar to the inferior-alveolar-canal (IAC). It is important to understand the reliability of conventional panoramic-radiograph in the assessment of this criterion since it is more commonly used as first line radiographic approach due to its availability and lower radiation dose. This study aimed to investigate the reliability of conventional panoramic-radiograph in the evaluation of the proximity of impacted mandibular-third-molar root tip to the IAC by correlating the results with CBCT. A total of 65 root tips of …

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Revisiting The Fiqh Of Halal-Haram Animals: An Analysis On Malaysian Fatwa Relating To Animal Care And Use, M.I. Mokhtar, S.M.I. Munir Jan 2017

Revisiting The Fiqh Of Halal-Haram Animals: An Analysis On Malaysian Fatwa Relating To Animal Care And Use, M.I. Mokhtar, S.M.I. Munir

Research Publications (2016 to 2020)

The recent trends in the field of scientific research welcomes the interpolation of alternative research ideologies and methodologies in ensuring that issues advocating the Animal Care and Use (ACU) be handled in an ethical and holistic manner. This research tends to provide a connection between conventional animal ethics and Islamic ethics as a complementary framework via the application of Fiqh halal-haram animals. The gist of discussion in this paper is based on the Malaysia context. This exploratory research invokes an exhaustive referencing of voluminous Islamic literature primary resources and in-depth interviews with four renowned Muftis. The number of Fatwas related …

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Serological And Molecular Identification Of Leptospira Spp. In Swine And Stray Dogs From Malaysia, D. Benacer, Kwai Lin Thong, P.T. Ooi, M. Souris, J.W. Lewis, A.A. Ahmed, S.N. Mohd Zain Jan 2017

Serological And Molecular Identification Of Leptospira Spp. In Swine And Stray Dogs From Malaysia, D. Benacer, Kwai Lin Thong, P.T. Ooi, M. Souris, J.W. Lewis, A.A. Ahmed, S.N. Mohd Zain

Research Publications (2016 to 2020)

Leptospirosis is endemic in Malaysia with Leptospira species extensively isolated from domestic and wild animals. Rats were found to be the principal maintenance hosts followed by cattle, pigs, and dogs. The objectives of this study were to isolate and identify Leptospira serovars circulating among swine from three different farms and also from stray dogs and cats from Klang valley, Selangor, Malaysia. Urine and kidney samples collected from 150 stray dogs, 50 cats and 81 swine were inoculated into semi-solid Ellinghausen McCullough Johnson and Harris (EMJH) media supplemented with additional 5-Fluorouracil. Dark field microscopy revealed only one positive culture of Leptospira …

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Spina Bifida: Pathogenesis, Mechanisms, And Genes In Mice And Humans, S.W. Mohd-Zin, A.I. Marwan, M.K. Abou Chaar, A. Ahmad-Annuar, N.M. Abdul-Aziz Jan 2017

Spina Bifida: Pathogenesis, Mechanisms, And Genes In Mice And Humans, S.W. Mohd-Zin, A.I. Marwan, M.K. Abou Chaar, A. Ahmad-Annuar, N.M. Abdul-Aziz

Research Publications (2016 to 2020)

Spina bifida is among the phenotypes of the larger condition known as neural tube defects (NTDs). It is the most common central nervous system malformation compatible with life and the second leading cause of birth defects after congenital heart defects. In this review paper, we define spina bifida and discuss the phenotypes seen in humans as described by both surgeons and embryologists in order to compare and ultimately contrast it to the leading animal model, the mouse. Our understanding of spina bifida is currently limited to the observations we make in mouse models, which reflect complete or targeted knockouts of …

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Suppression Of Microrna-629 Enhances Sensitivity Of Cervical Cancer Cells To 1€²S-1€²-Acetoxychavicol Acetate Via Regulating Rsu1, N.H. Phuah, M.N. Azmi, Khalijah Awang, Noor Hasima Nagoor Jan 2017

Suppression Of Microrna-629 Enhances Sensitivity Of Cervical Cancer Cells To 1€²S-1€²-Acetoxychavicol Acetate Via Regulating Rsu1, N.H. Phuah, M.N. Azmi, Khalijah Awang, Noor Hasima Nagoor

Research Publications (2016 to 2020)

Background: Cervical cancer is the fourth most frequent malignancy affecting women worldwide, but drug resistance and toxicities remain a major challenge in chemotherapy. The use of natural compounds is promising because they are less toxic and able to target multiple signaling pathways. The 1′S-1′-acetoxychavicol acetate (ACA), a natural compound isolated from wild ginger Alpinia conchigera, induced cytotoxicity on various cancer cells including cervical cancer. MicroRNAs (miRNAs) are short noncoding RNAs that regulate numerous biological processes, such as apoptosis and chemosensitivity. Past studies reported that miR-629 is upregulated in many cancers, and its expression was altered in ACA-treated cervical cancer cells. …

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Surface Functionalization Of Iron Oxide Nanoparticles With Gallic Acid As Potential Antioxidant And Antimicrobial Agents, S.T. Shah, W.A. Yehya, O. Saad, K. Simarani, Z.Z. Chowdhury, A.A. Alhadi, L.A. Al-Ani Jan 2017

Surface Functionalization Of Iron Oxide Nanoparticles With Gallic Acid As Potential Antioxidant And Antimicrobial Agents, S.T. Shah, W.A. Yehya, O. Saad, K. Simarani, Z.Z. Chowdhury, A.A. Alhadi, L.A. Al-Ani

Research Publications (2016 to 2020)

In this research, we report the size‐controlled synthesis and surface‐functionalization of magnetite with the natural antioxidant gallic acid (GA) as a ligand, using in situ and post‐synthesis methods. GA functionalization provided narrow size distribution, with an average particle size of 5 and 8 nm for in situ synthesis of gallic acid functionalized magnetite IONP@GA1 and IONP@GA2, respectively, which are ultra‐small particles as compared to unfunctionalized magnetite (IONP) and post functionalized magnetite IONP@GA3 with average size of 10 and 11 nm respectively. All the IONPs@GA samples were found hydrophilic with stable aggregation state. Prior to commencement of experimental lab work, PASS …

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Synthesis And Biological Evaluation Of Indole Core-Based Derivatives With Potent Antibacterial Activity Against Resistant Bacterial Pathogens, W. Hong, J. Li, Z. Chang, X. Tan, H. Yang, Y. Ouyang, Y. Yang, S. Kaur, I.C. Paterson, Y.F. Ngeow, H. Wang Jan 2017

Synthesis And Biological Evaluation Of Indole Core-Based Derivatives With Potent Antibacterial Activity Against Resistant Bacterial Pathogens, W. Hong, J. Li, Z. Chang, X. Tan, H. Yang, Y. Ouyang, Y. Yang, S. Kaur, I.C. Paterson, Y.F. Ngeow, H. Wang

Research Publications (2016 to 2020)

The emergence of drug resistance in bacterial pathogens is a growing clinical problem that poses difficult challenges in patient management. To exacerbate this problem, there is currently a serious lack of antibacterial agents that are designed to target extremely drug-resistant bacterial strains. Here we describe the design, synthesis and antibacterial testing of a series of 40 novel indole core derivatives, which are predicated by molecular modeling to be potential glycosyltransferase inhibitors. Twenty of these derivatives were found to show in vitro inhibition of Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus. Four of these strains showed additional activity against Gram-negative bacteria, including …

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The Apoptotic Effect Of 1’S-1’-Acetoxychavicol Acetate (Aca) Enhanced By Inhibition Of Non-Canonical Autophagy In Human Non-Small Cell Lung Cancer Cells, S.P.M. Sok, Norhafiza Mohd Arshad, M.N. Azmi, Khalijah Awang, B. Ozpolat, Noor Hasima Nagoor Jan 2017

The Apoptotic Effect Of 1’S-1’-Acetoxychavicol Acetate (Aca) Enhanced By Inhibition Of Non-Canonical Autophagy In Human Non-Small Cell Lung Cancer Cells, S.P.M. Sok, Norhafiza Mohd Arshad, M.N. Azmi, Khalijah Awang, B. Ozpolat, Noor Hasima Nagoor

Research Publications (2016 to 2020)

Autophagy plays a role in deciding the fate of cells by inducing either survival or death. 1’S-1-acetoxychavicol acetate (ACA) is a phenylpropanoid isolated from rhizomes of Alpinia conchigera and has been reported previously on its apoptotic effects on various cancers. However, the effect of ACA on autophagy remains ambiguous. The aims of this study were to investigate the autophagy-inducing ability of ACA in human non-small cell lung cancer (NSCLC), and to determine its role as pro-survival or pro-death mechanism. Cell viability assay was conducted using MTT. The effect of autophagy was assessed by acridine orange staining, GFP-LC3 punctate formation assay, …

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The Associations Between Vdr Bsmi Polymorphisms And Risk Of Vitamin D Deficiency, Obesity And Insulin Resistance In Adolescents Residing In A Tropical Country, R. Rahmadhani, N.L. Zaharan, Zahurin Mohamed, Foong Ming Moy, Muhammad Yazid Jalaludin Jan 2017

The Associations Between Vdr Bsmi Polymorphisms And Risk Of Vitamin D Deficiency, Obesity And Insulin Resistance In Adolescents Residing In A Tropical Country, R. Rahmadhani, N.L. Zaharan, Zahurin Mohamed, Foong Ming Moy, Muhammad Yazid Jalaludin

Research Publications (2016 to 2020)

Background: The vitamin D receptor (VDR) gene is expressed abundantly in different tissues; including adipocytes and pancreatic beta cells. The rs1544410 or BsmI single nucleotide polymorphism (SNP) in the intronic region of the VDR gene has been previously associated with vitamin D levels, obesity and insulin resistance. Aims: This study was aimed to examine the association between BsmI polymorphism and risk of vitamin D deficiency, obesity and insulin resistance in adolescents living in a tropical country. Methods: Thirteen-year-old adolescents were recruited via multistage sampling from twenty-three randomly selected schools across the city of Kuala Lumpur, Malaysia (n = 941). Anthropometric …

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The Effect Of Increasing The Percentage Of Hydrogen Added To Methane In Direct Injection Spark Ignition Engine To The Composition Of Co And Co2 Emissions, M.R.A. Mansor, C.L. Hoo, N.M. Taib, W.G.W. Aizon, Zul Ilham Jan 2017

The Effect Of Increasing The Percentage Of Hydrogen Added To Methane In Direct Injection Spark Ignition Engine To The Composition Of Co And Co2 Emissions, M.R.A. Mansor, C.L. Hoo, N.M. Taib, W.G.W. Aizon, Zul Ilham

Research Publications (2016 to 2020)

The emission of CO2 has been dramatically increased within the last decade and is still increasing each year, making it the main cause of global warming. CO2 is mainly created by burning fossil fuels such as petrol and diesel. One of the solutions to decrease the emission is by changing the use of petrol to alternative fuel such as Compressed Natural Gas (CNG) or hydrogen. CNG is primarily composed of methane and also contains ethane, propane and heavier hydrocarbons. Even though natural gas has the lowest carbon emission of all fossil fuel, it still has significant carbon content. On the …

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The First Molecular Survey Of Theileriosis In Malaysian Cattle, Sheep And Goats, K.L. Kho, A.D.G. Amarajothi, F.X. Koh, C. Panchadchram, Q.N.H. Nizam, S.T. Tay Jan 2017

The First Molecular Survey Of Theileriosis In Malaysian Cattle, Sheep And Goats, K.L. Kho, A.D.G. Amarajothi, F.X. Koh, C. Panchadchram, Q.N.H. Nizam, S.T. Tay

Research Publications (2016 to 2020)

This study reports the molecular detection of Theileria spp. from six cattle farms, a sheep farm and a goat farm located at different states in Peninsular Malaysia. Animal b lood samples were screened for the presence of Theileria DNA using a conventional polymerase chain reaction (PCR) assay. A total of 155 (69.2%) of 224 cattle investigated were PCR-positive for Theileria DNA. The occurrences of Theileria spp. ranged from 17.5% to 100.0% across six cattle farms. Theileria DNA was detected from 90.0% of 40 sheep but none of 40 goats examined in this study. Sequence analyses of amplified 18S rRNA partial …

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The Oral Microbiome Community Variations Associated With Normal, Potentially Malignant Disorders And Malignant Lesions Of The Oral Cavity, S.F. Mok, Karuthan Chinna, Y.K. Cheah, Wei Cheong Ngeow, R.B. Zain, S.F. Yap, H.K.A. Ong Jan 2017

The Oral Microbiome Community Variations Associated With Normal, Potentially Malignant Disorders And Malignant Lesions Of The Oral Cavity, S.F. Mok, Karuthan Chinna, Y.K. Cheah, Wei Cheong Ngeow, R.B. Zain, S.F. Yap, H.K.A. Ong

Research Publications (2016 to 2020)

The human oral microbiome has been known to show strong association with various oral diseases including oral cancer. This study attempts to characterize the community variations between normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences were processed and analysed with MOTHUR. A core oral microbiome was identified consisting of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and …

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The Us8, Us4, Es31, And Ul14 Ribosomal Protein Genes Are Dysregulated In Nasopharyngeal Carcinoma Cell Lines, E.U.H. Sim, K.L. Ng, C.W. Lee, K. Narayanan Jan 2017

The Us8, Us4, Es31, And Ul14 Ribosomal Protein Genes Are Dysregulated In Nasopharyngeal Carcinoma Cell Lines, E.U.H. Sim, K.L. Ng, C.W. Lee, K. Narayanan

Research Publications (2016 to 2020)

The association of ribosomal proteins with carcinogenesis of nasopharyngeal carcinoma (NPC) has been established in a limited subset of ribosomal protein genes. To date, three ribosomal protein genes, eL27 (L27), eL41 (L41), and eL43 (L37a), have been found to be differentially expressed in cell lines derived from NPC tumors. This raises the possibility of more ribosomal protein genes that could be associated with NPC. In this study, we investigated the expression profiles of eight ribosomal protein genes, uS8 (S8), uS4 (S9), eS31 (S27a), eL6 (L6), eL18 (L18), uL14 (L23), eL24 (L24), and eL30 (L30), in six NPC-derived cell lines (HONE-1, …

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Titanium Addition Influences Antibacterial Activity Of Bioactive Glass Coatings On Metallic Implants, O. Rodriguez, W. Stone, E.H. Schemitsch, P. Zalzal, S. Waldman, M. Papini, M.R. Towler Jan 2017

Titanium Addition Influences Antibacterial Activity Of Bioactive Glass Coatings On Metallic Implants, O. Rodriguez, W. Stone, E.H. Schemitsch, P. Zalzal, S. Waldman, M. Papini, M.R. Towler

Research Publications (2016 to 2020)

In an attempt to combat the possibility of bacterial infection and insufficient bone growth around metallic, surgical implants, bioactive glasses may be employed as coatings. In this work, silica-based and borate-based glass series were synthesized for this purpose and subsequently characterized in terms of antibacterial behavior, solubility and cytotoxicity. Borate-based glasses were found to exhibit significantly superior antibacterial properties and increased solubility compared to their silica-based counterparts, with BRT0 and BRT3 (borate-based glasses with 0 and 15 mol% of titanium dioxide incorporated, respectively) outperforming the remainder of the glasses, both borate and silicate based, in these respects. Atomic Absorption Spectroscopy …

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Transcriptome Profiling Of Monocytes From Xla Patients Revealed The Innate Immune Function Dysregulation Due To The Btk Gene Expression Deficiency, H. Mirsafian, A.M. Ripen, W.M. Leong, C.T. Chear, S.B. Mohamad, A.F. Merican Jan 2017

Transcriptome Profiling Of Monocytes From Xla Patients Revealed The Innate Immune Function Dysregulation Due To The Btk Gene Expression Deficiency, H. Mirsafian, A.M. Ripen, W.M. Leong, C.T. Chear, S.B. Mohamad, A.F. Merican

Research Publications (2016 to 2020)

X-linked agammaglobulinemia (XLA) is a rare genetic disorder, caused by mutations in BTK (Bruton's Tyrosine Kinase) gene. Deep high-throughput RNA sequencing (RNA-Seq) approach was utilized to explore the possible differences in transcriptome profiles of primary monocytes in XLA patients compared with healthy subjects. Our analysis revealed the differences in expression of 1,827 protein-coding genes, 95 annotated long non-coding RNAs (lncRNAs) and 20 novel lincRNAs between XLA patients and healthy subjects. GO and KEGG pathway analysis of differentially expressed (DE) protein-coding genes showed downregulation of several innate immune-related genes and upregulation of oxidative phosphorylation and apoptosis-related genes in XLA patients compared …

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Trends In Environmental Studies In Southeast Asia: Transboundary Haze, H. Varkkey Jan 2017

Trends In Environmental Studies In Southeast Asia: Transboundary Haze, H. Varkkey

Research Publications (2016 to 2020)

Environmental politics is part of an emerging and exciting field of study in Southeast Asia. The literature on politics and governance in Southeast Asia and ASEAN has for many years been overwhelmingly dominated by ‘hard’ issues of security and economics, and not so much by ‘soft’ issues like the environment. This is unsurprising considering that ASEAN was founded primarily to accelerate economic growth and political security in the region. However of course, hand in hand with the increased rates of environmental deterioration and pollution in various Southeast Asian countriesover the years, the academic attention to issues of the environment in …

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Viral Dynamics During A Phytoplankton Bloom Event, C.W. Lee, J.H. Lim, P.L. Heng Jan 2017

Viral Dynamics During A Phytoplankton Bloom Event, C.W. Lee, J.H. Lim, P.L. Heng

Research Publications (2016 to 2020)

Sampling was carried out in a tropical Klang estuary in order to observe for the periodic spikes in chlorophyll a (Chl a) concentration that is known to occur during rainy season. Sampling was at the Klang river mouth, and was from April until September 2010 (six samplings), from July until November 2011 (11 samplings) and from October until November 2012 (8 samplings). In the year 2011 and 2012, we observed spikes in Chl a concentration up to 26.3 μg l–1 (2011) and 22.9 μg l–1 (2012). Viral abundance was observed as virus like particles (VLP) via epifluorescence microscopy after staining …

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Vitamin E (-Tocopherol) Exhibits Antitumour Activity On Oral Squamous Carcinoma Cells Orl-48, R. Zulkapli, Fathilah Abdul Razak, R.B. Zain Jan 2017

Vitamin E (-Tocopherol) Exhibits Antitumour Activity On Oral Squamous Carcinoma Cells Orl-48, R. Zulkapli, Fathilah Abdul Razak, R.B. Zain

Research Publications (2016 to 2020)

Cancers involving the oral cavity, head, and neck regions are often treated with cisplatin. In cancer therapy, the main target is to eliminate unwanted cancerous cells. However, reports on the nonselective nature of this drug have raised few concerns. Incorrect nutritional habits and lifestyle practices have been directly linked to cancer incidence. Nutrients with antioxidant activity inhibit cancer cells development, destroying them through oxidative stress and apoptosis. -tocopherol, the potent antioxidant form of vitamin E is a known scavenger of free radicals. In vitro study exhibited effective antitumor activity of -tocopherol on ORL-48 at 2.5 +/- 0.42 mu g/mL. Cisplatin …

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Whole-Genome Phylogenetic Analysis Of Influenza B/Phuket/3073/2013-Like Viruses And Unique Reassortants Detected In Malaysia Between 2012 And 2014, X.Y. Oong, K.T. Ng, J.L. Tan, Kok Gan Chan, Adeeba Kamarulzaman, Yoke Fun Chan, I-Ching Sam, Kok Keng Tee Jan 2017

Whole-Genome Phylogenetic Analysis Of Influenza B/Phuket/3073/2013-Like Viruses And Unique Reassortants Detected In Malaysia Between 2012 And 2014, X.Y. Oong, K.T. Ng, J.L. Tan, Kok Gan Chan, Adeeba Kamarulzaman, Yoke Fun Chan, I-Ching Sam, Kok Keng Tee

Research Publications (2016 to 2020)

Reassortment of genetic segments between and within influenza B lineages (Victoria and Yamagata) has been shown to generate novel reassortants with unique genetic characteristics. Based on hemagglutinin (HA) and neuraminidase (NA) genes, recent surveillance study has identified reassortment properties in B/Phuket/3073/2013-like virus, which is currently used in the WHO-recommended influenza vaccine. To understand the potential reassortment patterns for all gene segments, four B/Phuket/3073/2013-like viruses and two unique reassortants (one each from Yamagata and Victoria) detected in Malaysia from 2012-2014 were subjected to whole-genome sequencing. Each gene was phylogenetically classified into lineages, clades and sub-clades. Three B/Phuket/3073/2013-like viruses from Yamagata lineage …

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Genetic Analysis Of Serf Gene Function In Drosophila Melanogaster And Its Contribution To A Fly Model Of Spinal Muscular Atrophy, Swagata Ghosh Jan 2017

Genetic Analysis Of Serf Gene Function In Drosophila Melanogaster And Its Contribution To A Fly Model Of Spinal Muscular Atrophy, Swagata Ghosh

Theses and Dissertations--Biology

The Serf gene is evolutionarily highly conserved but its biological function is not known in any organism. In human, SERF1/H4F5 was first identified as a modifier of the disease Spinal Muscular Atrophy (SMA). SMA is caused by mutations in the Survival Motor Neuron 1(SMN1) gene leading to diminished levels of the Smn protein. More than 90% of patients with the most severe form of SMA have deletions that remove SERF1 as well as mutaions within SMN1. Hence, loss of Serf activity is hypothesized to exacerbate SMA disease progression. The primary motivation of this thesis was to test …

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Protein Found At The Scene Of The Crime: The Potential For Using Proteomics For Identification, Gavin R. Tisdale Jan 2017

Protein Found At The Scene Of The Crime: The Potential For Using Proteomics For Identification, Gavin R. Tisdale

Dissertations and Honors Papers

Hair has long been collected from crime scenes as part of trace evidence. Originally, hair was used for some exclusionary purposes—only general qualities about an unknown source could be determined. Eventually, DNA was used to help identify the source but only if the root was still attached. Within the last two years, however, two major studies have used proteomics—the study of human protein sequences—to extract and identify protein sequences in an unknown source in order to match it to a known source. These two studies support the same hypothesis: proteomics is currently a viable method for narrowing down the source …

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Report On The 26th International Liquid Crystal Conference, Peter J. Collings Jan 2017

Report On The 26th International Liquid Crystal Conference, Peter J. Collings

Physics & Astronomy Faculty Works

No abstract provided.

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Redd Habitat Selection For Brook Trout In The Necktie River Bemidji, Mn, William Varela, Zachary Buckley Jan 2017

Redd Habitat Selection For Brook Trout In The Necktie River Bemidji, Mn, William Varela, Zachary Buckley

Journal of Earth and Life Science

The presence of quality suitable habitat is important for the success of naturally reproducing Brook Trout populations. Therefore, measuring redd habitat Brook Trout select for was the focus of this study. Brook Trout redds were observed during the spawning season of 2016. We identified a total of 18 redds in the Necktie River in late October and early November. Specific environmental variables associated with redd selection were measured: velocity, in-stream cover, canopy cover, bank overhang, river width, substrate type and size, temperature, and max-depth. The same environmental variables were measured for 30 randomly selected sites. Redds were distributed in riffles …

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Influence Of Time Spent In Captivity On Aggressive Behaviors Of Gray Wolves Canis Lupus, Chelsea Knutson Jan 2017

Influence Of Time Spent In Captivity On Aggressive Behaviors Of Gray Wolves Canis Lupus, Chelsea Knutson

Journal of Earth and Life Science

Knowledge of the characteristics that promote group success is key to understanding the adaptive advantages of sociality for many group-oriented species. In this study, nine Gray Wolves Canis lupis were each observed twelve times over a period of six months to determine if the amount of time spent in the wild/captivity had any effect on aggressive behaviors. The number of aggressive behaviors were recorded for each wolf during each observation. The nine wolves were separated into three groups depending on their upbringing; born and raised in captivity, born in the wild and raised in captivity, and born and raised in …

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Gvsu Press Releases, 2017, Grand Valley State University Jan 2017

Gvsu Press Releases, 2017, Grand Valley State University

University Press Releases, 1961-Present

A compilation of press releases for the year 2017 submitted by University Communications (formerly News & Information Services) to news agencies concerning the people, places, and events related to Grand Valley State University.

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Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota Jan 2017

Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota

Department of Animal Science: Faculty Publications

Background: High-density marker panels and/or whole-genome sequencing, coupled with advanced phenotyping pipelines and sophisticated statistical methods, have dramatically increased our ability to generate lists of candidate genes or regions that are putatively associated with phenotypes or processes of interest. However, the speed with which we can validate genes, or even make reasonable biological interpretations about the principles underlying them, has not kept pace. A promising approach that runs parallel to explicitly validating individual genes is analyzing a set of genes together and assessing the biological similarities among them. This is often achieved via gene ontology analysis, a powerful tool that …

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Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White Jan 2017

Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient. One GNRHR2 knockdown (KD) female was born that subsequently produced 80 piglets from 6 litters with 46 hemizygous …

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