Digital Commons Network^™

Fluorogenic Protein Labeling Using A Genetically Encoded Unstrained Alkene, X. Shang, X. Song, C. Faller, R. Lai, H. Li, Ronald Cerny, Wei Niu, Jiantao Guo

Department of Chemistry: Faculty Publications

We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels–Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a styrene-containing amino acid into proteins in response to amber nonsense codon. Fluorogenic labeling of purified proteins and intact proteins in live cells were demonstrated. The fluorogenicity of the styrene–tetrazine reaction can be potentially applied to the study of protein folding and function under physiological conditions with low background fluorescence interference.

Woody Ornamental Disease Management Research Reports 2017, Fulya Baysal-Gurel

Extension Publications

No abstract provided.

Polymeric Spatial Resolution Test Patterns For Mass Spectrometry Imaging Using Nano‐Thermal Analysis With Atomic Force Microscopy, Tamin Tai, Vilmos Kertesz, Ming-Wei Lin, Bernadeta R. Srijanto, Dale K. Hensley, Kai Xiao, Gary J. Van Berkel

United States Department of Energy: Publications

RATIONALE: As the spatial resolution of mass spectrometry imaging technologies has begun to reach into the nanometer regime, finding readily available or easily made resolution reference materials has become particularly challenging for molecular imaging purposes. This paper describes the fabrication, characterization and use of vertical line array polymeric spatial resolution test patterns for nano‐thermal analysis/atomic force microscopy/mass spectrometry chemical imaging.

METHODS: Test patterns of varied line width (0.7 or 1.0 μm) and spacing (0.7 or 1.0 μm) were created in an ~1‐μm‐thick poly(methyl methacrylate) thin film using electron beam lithography. The patterns were characterized by scanning electron microscopy, …

Multiple Method Analysis Of Tio2 Nanoparticle Uptake In Rice (Oryza Sativa L.) Plants, Yingqing Deng, Elijah J. Petersen, Katie E. Challis, Savelas A. Rabb, R. David Holbrook, James F. Ranville, Bryant C. Nelson, Baoshan Xing

United States Department of Commerce: Staff Publications

Understanding the translocation of nanoparticles (NPs) into plants is challenging because qualitative and quantitative methods are still being developed and the comparability of results among different methods is unclear. In this study, uptake of titanium dioxide NPs and larger bulk particles (BPs) in rice plant (Oryza sativa L.) tissues was evaluated using three orthogonal techniques: electron microscopy, single-particle inductively coupled plasma mass spectroscopy (spICP-MS) with two different plant digestion approaches, and total elemental analysis using ICP optical emission spectroscopy. In agreement with electron microscopy results, total elemental analysis of plants exposed to TiO₂ NPs and BPs at 5 …

Perspectives On Climate Change Mitigation, Benjamin Deangelo, James Edmonds, David Fahey, Benjamin Sanderson

United States Department of Commerce: Staff Publications

1. Warming and associated climate effects from CO2 3 emissions persist for decades to millennia. In the near-term, changes in climate are determined by past and present greenhouse gas emissions modified by natural variability. Reducing the total concentration of atmospheric CO2 is necessary to limit near-term climate change and stay below long-term warming targets (such as the oft-cited 3.6°F [2°C] goal). Other greenhouse gases (for example, methane) and black carbon aerosols exert stronger warming effects than CO2 on a per ton basis, but they do not persist as long in the atmosphere; therefore, mitigation of non-CO2 species contributes substantially to …

Ocean Changes – Warming, Stratification, Circulation, Acidification, And Deoxygenation, Libby Jewett, Anastasia Romanou

United States Department of Commerce: Staff Publications

1. The world’s oceans have absorbed about 93% of the excess heat caused by greenhouse gas warming since the mid-20th century, making them warmer and altering global and regional climate feedbacks. Ocean heat content has increased at all depths since the 1960s and surface waters have warmed by about 1.3° ± 0.1°F (0.7° ± 0.08°C) per century globally since 1900 to 2016. Under a high emissions scenario, a global increase in average sea surface temperature of 4.9° ± 1.3°F (2.7° ± 0.7°C) by 2100 is projected, with even higher changes in some U.S. coastal regions. (Very high confidence) …

Climate Models, Scenarios, And Projections, Katharine Hayhoe, James Edmonds, Robert Kopp, Allegra Legrande, Benjamin Sanderson, Michael Wehner, Donald Wuebbles

United States Department of Commerce: Staff Publications

1. If greenhouse gas concentrations were stabilized at their current level, existing concentrations would commit the world to at least an additional 1.1°F (0.6°C) of warming over this century relative to the last few decades (high confidence in continued warming, medium confidence in amount of warming).

2. Over the next two decades, global temperature increase is projected to be between 0.5°F and 1.3°F (0.3°–0.7°C) (medium confidence). This range is primarily due to uncertainties in natural sources of variability that affect short-term trends. In some regions, this means that the trend may not be distinguishable from natural variability ( …

Fly Models Of Human Diseases: Drosophila As A Model For Understanding Human Mitochondrial Mutations And Disease, A. Sen, R.T. Cox

Uniformed Services University of the Health Sciences

Mitochondrial diseases are a prevalent, heterogeneous class of diseases caused by defects in oxidative phosphorylation, whose severity depends upon particular genetic mutations. These diseases can be difficult to diagnose, and current therapeutics have limited efficacy, primarily treating only symptoms. Because mitochondria play a pivotal role in numerous cellular functions, especially ATP production, their diminished activity has dramatic physiological consequences. While this in and of itself makes treating mitochondrial disease complex, these organelles contain their own DNA, mtDNA, whose products are required for ATP production, in addition to the hundreds of nucleus-encoded proteins. Drosophila offers a tractable whole-animal model to understand …

Effects Of Isoflurane Anesthesia And Intravenous Morphine Self-Administration On Regional Glucose Metabolism ([18F]Fdg-Pet) Of Male Sprague-Dawley Rats, Thomas Y. Park, Kevin S. Nishida, Colin M. Wilson, Shalini Jaiswal, Jessica Scott, Andrew R. Hoy, Reed G. Selwyn, Bernard J. Dardzinski, Kwang H. Choi

Uniformed Services University of the Health Sciences

Although certain drugs of abuse are known to disrupt brain glucose metabolism (BGluM), the effects of opiates on BGluM are not well characterized. Moreover, preclinical positron emission tomography (PET) studies anesthetize animals during the scan, which limits clinical applications. We investigated the effects of (i) isoflurane anesthesia and (ii) intravenous morphine self-administration (MSA) on BGluM in rats. Jugular vein cannulated adult male Sprague-Dawley rats self-administered either saline (SSA) or morphine (0.5 mg/kg/infusion, 4 h/day for 12 days). All animals were scanned twice with [18F]-fluoro-deoxyglucose (FDG)-PET/CT at a baseline and at 2-day withdrawal from self-administration. After the IV injection of FDG, …

Population Based Mri And Dti Templates Of The Adult Ferret Brain And Tools For Voxelwise Analysis, E.B. Hutchinson, S.C. Schwerin, K.L. Radomski, N. Sadeghi, J. Jenkins, M.E. Komlosh, M.O. Irfanoglu, S.L. Juliano, C. Pierpaoli

Uniformed Services University of the Health Sciences

Non-invasive imaging has the potential to play a crucial role in the characterization and translation of experimental animal models to investigate human brain development and disorders, especially when employed to study animal models that more accurately represent features of human neuroanatomy. The purpose of this study was to build and make available MRI and DTI templates and analysis tools for the ferret brain as the ferret is a well-suited species for pre-clinical MRI studies with folded cortical surface, relatively high white matter volume and body dimensions that allow imaging with pre-clinical MRI scanners. Four ferret brain templates were built in …

Hypothesis: Exosomal Micrornas As Potential Biomarkers For Schizophrenia, Vijaya Raghavan, Manish Bhomia, Isabel Torres, Sanjeev Jain, Kevin K. Wang

Uniformed Services University of the Health Sciences

Schizophrenia is a serious mental disorder with lifelong morbidity and increased mortality. Currently, the diagnosis of the disorder is based on patient history and clinical examination, but it has a low inter-rater reliability and validity. Various biological variables, such as event related potentials, hormonal levels, brain ventricular volume and hippocampal size, have been put forth as objective markers to diagnose schizophrenia, but none with the desired sensitivity and specificity. It has been shown that microRNAs play a vital role in gene regulation in schizophrenia and have been proposed as possible biomarkers for the disease. When compared to the free microRNAs …

Multidimensional Assessment Of Impulsivity In Relation To Obesity And Food Addiction, Lauren Vanderbroek-Stice, Monika Stojek, Steven R.H. Beach, Michelle R. Vandellen, James Mackillop

Uniformed Services University of the Health Sciences

Based on similarities between overconsumption of food and addictive drugs, there is increasing interest in “food addiction,” a compulsive eating pattern defined using symptoms parallel to substance use disorders. Impulsivity, a multidimensional construct robustly linked to drug addiction, has been increasingly examined as an obesity determinant, but with mixed findings. This study sought to clarify relations between three major domains of impulsivity (i.e., impulsive personality traits, discounting of delayed rewards, and behavioral inhibition) in both obesity and food addiction. Based on the association between impulsivity and compulsive drug use, the general hypothesis was that the impulsivity-food addiction relation would be …

Examination Of The Interpersonal Model Of Loss Of Control Eating In The Laboratory, Lisa M. Shank, Ross D. Crosby, Anne Claire Grammer, Lauren B. Shomaker, Anna Vannucci, Natasha L. Burke, Monika Stojek, Sheila M. Brady, Merel Kozlosky, James C. Reynolds, Jack A. Yanovski, Marian Tanofsky-Kraff

Uniformed Services University of the Health Sciences

Background: The interpersonal model of loss of control (LOC) eating proposes that interpersonal problems lead to negative affect, which in turn contributes to the onset and/or persistence of LOC eating. Despite preliminary support, there are no data examining the construct validity of the interpersonal model of LOC eating using temporally sensitive reports of social stress, distinct negative affective states, and laboratory energy intake.

Method: 117 healthy adolescent girls (BMI: 75th–97th %ile) were recruited for a prevention trial targeting excess weight gain in adolescent girls who reported LOC eating. Prior to the intervention, participants completed questionnaires of recent social stress …

Association Of A Frailty Screening Initiative With Postoperative Survival At 30, 180, And 365 Days, Daniel E. Hall, Shipra Arya, Kendra K. Schmid, Mark A. Carlson, Pierre Lavedan, Travis L. Bailey, Georgia Purviance, Tammy Bockman, Thomas G. Lynch, Jason M. Johanning

United States Department of Veterans Affairs: Publications

IMPORTANCE As the US population ages, the number of operations performed on elderly patients will likely increase. Frailty predicts postoperative mortality and morbidity more than age alone, thus presenting opportunities to identify the highest-risk surgical patients and improve their outcomes.

OBJECTIVE To examine the effect of the Frailty Screening Initiative (FSI) on mortality and complications by comparing the surgical outcomes of a cohort of surgical patients treated before and after implementation of the FSI.

DESIGN, SETTING, AND PARTICIPANTS This single-site, facility-wide, prospective cohort quality improvement project studied all 9153 patients from a level 1b Veterans Affairs medical center who presented …

Smartphone Technologies And Bayesian Networks To Assess Shorebird Habitat Selection, Sara L. Zeigler, E. Robert Thieler, Benjamin T. Gutierrez, Nathaniel G. Plant, Megan Hines, James D. Fraser, Daniel H. Catlin

United States Geological Survey: Staff Publications

Understanding patterns of habitat selection across a species’ geographic distribution can be critical for adequately managing populations and planning for habitat loss and related threats. However, studies of habitat selection can be time consuming and expensive over broad spatial scales, and a lack of standardized monitoring targets or methods can impede the generalization of site-based studies. Our objective was to collaborate with natural resource managers to define available nesting habitat for piping plovers (Charadrius melodus) throughout their U.S. Atlantic coast distribution from Maine to North Carolina, with a goal of providing science that could inform habitat management in …

Channel-Planform Evolution In Four Rivers Of Olympic National Park, Washington, Usa: The Roles Of Physical Drivers And Trophic Cascades, Amy E. East, Kurt J. Jenkins, Patricia J. Happe, Jennifer A. Bountry, Timothy J. Beechie, Mark C. Mastin, Joel B. Sankey, Timothy J. Randle

United States Geological Survey: Staff Publications

Identifying the relative contributions of physical and ecological processes to channel evolution remains a substantial challenge in fluvial geomorphology. We use a 74-year aerial photographic record of the Hoh, Queets, Quinault, and Elwha Rivers, Olympic National Park, Washington, USA, to investigate whether physical or trophic-cascade-driven ecological factors – excessive elk impacts after wolves were extirpated a century ago – are the dominant drivers of channel planform in these gravel-bed rivers.We find that channel width and braiding show strong relationships with recent flood history. All four rivers widened significantly after having been relatively narrow in the 1970s, consistent with increased flood …

Semi-Arid Grassland Bird Responses To Patch-Burn Grazing And Drought, Susan K. Skagen, David J. Augustine, Justin D. Derner

United States Geological Survey: Staff Publications

As grassland birds of central North America experience steep population declines with changes in land use, management of remaining tracts becomes increasingly important for population viability. The integrated use of fire and grazing may enhance vegetation heterogeneity and diversity in breeding birds, but the subsequent effects on reproduction are unknown. We examined the influence of patch-burn grazing management in shortgrass steppe in eastern Colorado on habitat use and reproductive success of 3 grassland bird species, horned lark (Eremophila alpestris), lark bunting (Calamospiza melanocorys), and McCown’s longspur (Rhynchophanes mccownii), at several spatial scales during 2011 …

Lack Of Observed Movement Response To Lead Exposure Of California Condors, Sharon A. Poessel, Joseph Brandt, Linda Uyeda, Molly Astell, Todd E. Katzner

United States Geological Survey: Staff Publications

Lead poisoning is an important conservation concern for wildlife, and scavenging birds are especially at risk from consumption of carcasses of animals killed with lead ammunition. Because current methods to identify lead exposure require animal capture and blood collection, management would benefit from the development of a less costly and noninvasive behavioral test for illness in wild animals. We attempted to design such a test to identify lead exposure in California condors (Gymnogyps californianus) that we tracked with global positioning system (GPS) telemetry in southern California, USA, 2013–2016. We measured blood-lead concentrations in tracked birds and expected that …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 18 2017, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

The Racial Identity Conflict: Diverse Perspectives On The Minimization Of The Stem Education Gap That Impacts Gifted And Talented Black Male Students!, Adrienne Coleman

Publications & Research

Research suggests that a cognitive gap begins as early as nine months of age in Black males, widens by two years of age, and by fifth grade cognition lags by nearly two full years (ETS, 2011). This gap is a result of multiple facets: “Children are suffering from a toxic cocktail of poverty, illiteracy, racial discrimination and massive incarceration that sentences poor boys to dead-end hopeless lives” (ETS, 2011, p. 2). The outcome is the Black male being inadequately educated and academically behind other subcultures of the population. Lack of family support, poor school systems with inadequate resources, peer pressure, …

Geographic Distribution: Acrantophis Dumerili (Duméril’S Madagascan Ground Boa). Usa. Florida., Louis A. Somma, Kenneth L. Krysko, Laurence L. Conner

Papers in Herpetology

The geographic distribution of Acrantophis dumerili, a nonindigenous introduced species in Florida, with new records, is presented.

Acrantophis dumerili is indigenous to southern and southwestern Madagascar. We provide the first vouchers for Miami-Dade and Bay counties, which were likely escaped or released pets. Florida already has three established species of booid snakes (one boa, two pythons), all introduced through the pet trade pathway. Although there is no clear evidence for an established population of A. dumerili in Florida at this time, the Bay County individuals in a rural area in the Florida panhandle are a particular concern given that two …

The Status Of Two Species Of Nonindigenous Lizards In Florida, The Slender Chameleon, Chamaeleo Gracilis Hallowell 1842 And White-Lined Chameleon, Furcifer Lateralis (Gray 1831): A Corrigendum., Kenneth L. Krysko, Louis A. Somma

Papers in Herpetology

Krysko et al. (2016) recently published a summary of all known verified introductions of nonindigenous amphib­ians and reptiles in Florida, USA. Herein, we correct the iden­tity tity of one species and include an additional new record of a nonindigenous chameleon in Florida.

Krysko et al. (2016) reported the presence of a White-lined Chameleon, Furcifer lateralis (Gray 1831). In fact, this was a misidentified Slender Chameleon, Chamaeleo gracilis Hallowell 1842 (C. Anderson, pers. comm. 2016). Discussion and reference to F. lateralis in Krysko et al. (2016) and all associated data (UF voucher number, locality, collector, and collection date, including Table 1, …

Cross-Species Transmission Potential Between Wild Pigs, Livestock, Poultry, Wildlife, And Humans: Implications For Disease Risk Management In North America, Ryan S. Miller, Steven J. Sweeney, Chris Slootmaker, Daniel A. Grear, Paul A. Di Salvo, Deborah Kiser, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

Cross-species disease transmission between wildlife, domestic animals and humans is an increasing threat to public and veterinary health. Wild pigs are increasingly a potential veterinary and public health threat. Here we investigate 84 pathogens and the host species most at risk for transmission with wild pigs using a network approach. We assess the risk to agricultural and human health by evaluating the status of these pathogens and the co-occurrence of wild pigs, agriculture and humans. We identified 34 (87%) OIE listed swine pathogens that cause clinical disease in livestock, poultry, wildlife, and humans. On average 73% of bacterial, 39% of …

Seasonal Effects Of Wind Conditions On Migration Patterns Of Soaring American White Pelican, Javier Gutierrez Illan, Guiming Wang, Fred L. Cunningham, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

Energy and time expenditures are determinants of bird migration strategies. Soaring birds have developed migration strategies to minimize these costs, optimizing the use of all the available resources to facilitate their displacement. We analyzed the effects of different wind factors (tailwind, turbulence, vertical updrafts) on the migratory flying strategies adopted by 24 satellite-tracked American white pelicans (Pelecanus erythrorhynchos) throughout spring and autumn in North America. We hypothesize that different wind conditions encountered along migration routes between spring and autumn induce pelicans to adopt different flying strategies and use of these wind resources. Using quantile regression and fine-scale atmospheric …

Evaluating The Impact Of An Exotic Plant Invasion On Rodent Community Richness And Abundance, Trinity N. Smith, Eric M. Gese, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Cheatgrass (Bromus tectorum), Russian thistle (Salsola kali), and tall tumblemustard (Sisymbrium altissimum) are nonnative plants widely distributed throughout the desert and shrubsteppe communities of the western United States. The impact of these invaders on plant community structure, form, and function has been well documented, but investigations determining the impacts of this cumulative invasion on terrestrial vertebrates have not been undertaken. Our objective was to assess community-level rodent responses to changes in plant community features, with an emphasis on dominance of invasive plant species. We sampled rodent and plant communities in the Great Basin Desert …

The Effects Of Demographic, Social, And Environmental Characteristics On Pathogen Prevalence In Wild Felids Across A Gradient Of Urbanization, Jesse S. Lewis, Kenneth A. Logan, Mat W. Alldredge, Scott Carver, Sarah N. Bevins, Michael Lappin, Sue Vandewoude, Kevin R. Crooks

United States Department of Agriculture Wildlife Services: Staff Publications

Transmission of pathogens among animals is influenced by demographic, social, and environmental factors. Anthropogenic alteration of landscapes can impact patterns of disease dynamics in wildlife populations, increasing the potential for spillover and spread of emerging infectious diseases in wildlife, human, and domestic animal populations. We evaluated the effects of multiple ecological mechanisms on patterns of pathogen exposure in animal populations. Specifically, we evaluated how ecological factors affected the prevalence of Toxoplasma gondii (Toxoplasma), Bartonella spp. (Bartonella), feline immunodeficiency virus (FIV), and feline calicivirus (FCV) in bobcat and puma populations across wildlandurban interface (WUI), low-density exurban development, and wildland habitat …

Just For Arts & Sciences Career Networking Week, Jan 30 To Feb 3, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

My Summer As An Intern How To Find Yours, Feb 1, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Effects Of Socs1 And Socs3 Peptide Mimetics On Macrophage Phagocytosis Of Malignant Cells, Colt Dylan Capan

Browse all Theses and Dissertations

Macrophages play a key role in both the innate and adaptive immune system responses to foreign materials. Suppressors of cytokine signaling (SOCS) proteins are known as regulators of the immune response through various JAK/STAT pathways. This study examined the roles of SOCS1 and SOCS3 peptide mimetics on the phagocytosis of fluorescently labeled malignant target cell by RAW264.7 murine macrophages. The malignant cells used were Neuro-2a cells, a murine neuroblastoma cell line. A prominent “eat me” signal found in neuroblastoma cells is calreticulin (CRT), which permits macrophages to recognize and then phagocytize the malignant cells. When M1 (pro-inflammatory) polarized macrophages were …

Suffolk Journal, Vol. 81, No. 6, 10/18/2017, Suffolk Journal

Suffolk Journal

No abstract provided.