Digital Commons Network^™

Examination Of The Role Of Religious And Psychosocial Factors In Hiv Medication Adherence Rates, Safiya George Dalmida, Katryna Mccoy, Harold C. Koenig, Aretha Miller, Marcia Mcdonnell Holstad, Tami Thomas, Dora L. Clayton-Jones, Mary Margaret Grant, Terri Fleming, Menka Munira Wirani, George Mugoya

College of Nursing Faculty Research and Publications

Optimal adherence to antiretroviral therapy (ART) is associated with favorable HIV outcomes, including higher CD4 cell counts, HIV virus suppression and a lower risk of HIV transmission. However, only 25% of people living with HIV/AIDS (PLWH) in the USA are virally suppressed. Sub-optimal adherence (p< 0.05). Social support satisfaction was also significantly associated with ART adherence (OR = 1.52, 95% CI [1.11–2.08], p < 0.05) and energy/fatigue/vitality (OR = 1.03, 95% CI [1.00–1.05], p < 0.05).

Measurement Of Peptoid Concentration Using Uv Spectroscopy, Katie Holland

Chemical Engineering Undergraduate Honors Theses

The goal of this research is to establish a relationship between ultraviolet adsorption and peptiod concentration to be used to determine the concentration of an unknown peptoid sample. This will allow for the measurement of concentration differences of peptoid samples before and after incubation with membrane surfaces. Samples were prepared nine know concentrations and diluted 50 fold. The UV absorbances of each sample was measured using a UV spectrophotometer at wavelengths from 200 to 450 nanometers. This process was repeated, and the maximum absorbance of each replicate was averaged to yield an absorbance value for each concentration of peptoid. …

A Risk-Based Method For Estimating The Carbon Sequestration Budget For A Mixed Hardwood Forest, Erin Cooper

Chemical Engineering Undergraduate Honors Theses

Carbon Dioxide (CO₂) levels in the atmosphere have risen from approximately 310 ppm in the 1950’s to over 400 ppm as of 2015. This rise in CO₂ has likely resulted in the observed warming trend of the earth’s atmosphere in the same time frame, causing significant concern in the scientific community. Several mitigation strategies have arisen to combat the upward trend of CO₂ emissions in recent decades, among them being carbon sequestration- the process of capturing CO₂ from the atmosphere and holding it for an extended period of time. This study used the U.S. Forest …

A Comparative Life Cycle Assessment Of Nutritionally Equivalent Meals With And Without Pork, Kelli Young

Graduate Theses and Dissertations

The following report details a life cycle assessment of several dietary and meal scenarios with and without pork. The goal of the LCA was to identify the impacts of greenhouse gas emissions, water use, and land use of pork containing and porkless diets and meal plans in a field-to-fork analysis. The dietary and meal plan scenarios are iso-caloric meaning they contain the same number of calories. The first set of diets is based on a USDA consumption pattern, 2000 kcal per day. This diet was used to create three other dietary scenarios with and without pork. The USDA recommended food …

Peptoid-Modified Bicelles As Surrogate Cell Membranes For Membrane Protein Sensors And Analytics, Helya Najafi

Graduate Theses and Dissertations

Membrane-affiliated interactions are significant in understanding cell function, detecting biomarkers to diagnose disease, and in testing the efficiency of new therapeutic targets. Model membrane systems have been developed to study membrane proteins, allowing for stable protein structure and maintaining native activity. Bicelles, disc-shaped lipid bilayers created by combining long- and short-chain phospholipids, are the model membrane system of focus in this study. Bicelles are accessible from both sides and have a wide size range, which make them attractive for studying membrane proteins without affecting function. In this work, bicelles were functionalized with two peptoids to alter the edge and face …

The Potential Of Raman Spectroscopy In The Diagnosis Of Premalignant Oral Lesions, Ola Ibrahim

Doctoral

Thesis submitted in partial fulfilment for the degree of Doctor of Philosophy (PhD) Technological University Dublin, School of Physics December 2017.

Effect Of Endophyte-Infected Tall Fescue Toxins On Growth Performance And The Microbial Community In The Rumen And Feces In Pregnant Ewes, Saleh Alrashedi

Graduate Theses and Dissertations

Fifty pregnant Katahdin ewes grazed pastures with high (HE) and moderate (MI) levels of Neotyphodium coenophialum-infected tall fescue between February 1 and May 24 to determine the impacts of tall fescue toxins on body weight (BW), body condition scores, FAMACHA scores, fecal egg counts (FEC), and serum Cu, Zn, prolactin, and non-esterified fatty acids (NEFA). Within those 50 ewes, 15 were selected randomly for fecal and rumen sampling to assess the impacts of HE and MI on the microbiome community. Total bacterial DNA was extracted from rumen and fecal samples, and alpha and beta diversities were used to relate microbiome …

Polaron Dynamics In Conjugated Polymer Nanoparticles And Applications In Superresolution Imaging, Yifei Jiang

All Dissertations

Charge carrier dynamics in conjugated polymers are of fundamental interest as they directly affect the performance of conjugated polymer based devices. Through efficient fluorescence quenching by hole polarons, dynamics of individual charge carriers, including generation, recombination, and transport, can be observed through single particle fluorescence study of conjugated polymers nanoparticles (CPNs). In this dissertation, the hopping dynamics of hole polarons in CPNs were studied using superresolution microscopy. Due to quenching of the local fluorescence, a hole polaron can form a “dark spot” that moves with the polaron and results in displacements in the fluorescence centroid. These position fluctuations were used …

Ant-Inspired Interaction Networks For Decentralized Vehicular Traffic Congestion Control, Andreas Kasprzok

All Dissertations

Mimicking the autonomous behaviors of animals and their adaptability to changing or foreign environments lead to the development of swarm intelligence techniques such as ant colony optimization (ACO) and particle swarm optimization (PSO) now widely used to tackle a variety of optimization problems. The aim of this dissertation is to develop an alternative swarm intelligence model geared toward decentralized congestion avoidance and to determine qualities of the model suitable for use in a transportation network.

A microscopic multi-agent interaction network inspired by insect foraging behaviors, especially ants, was developed and consequently adapted to prioritize the avoidance of congestion, evaluated as …

Multifunctional Polymeric Micelles For Drug Resistant Breast Cancer: Self-Assembling Poly(Lactide-Co-Glycolide)-Graft-Polyethylenimine, Graham Mackenzie Temples

All Dissertations

Breast cancer is the most common malignancy and the leading cause of cancer death in women. Systemic breast cancer therapies include 1) hormone therapy, 2) immunotherapy, and 3) chemotherapy. Chemotherapy is commonly used in combination with immunotherapy, achieving synergistic activity by multiple mechanisms specific to the type of breast cancer. However, the efficacy of anticancer drugs has been limited by their toxic side effects in normal cells and drug resistance acquired by cancer cells. Therefore, the development of a novel treatment strategy for the selective delivery of therapeutic agents to breast cancer cells is crucial to improve the therapeutic index …

Micro-Cantilever Based Fiber Optic Hydrophone, Jie Liu

All Dissertations

Endoscopic photoacoustic imaging probe is becoming increasingly important for many clinical photoacoustic imaging applications in which the target tissue can only be accessed by introducing an endoscopic probe percutaneously or through a natural orifice. Miniature fiber optic hydrophone (FOH) has become an attractive choice for endoscopic photoacoustic imaging application. Fiber optic hydrophone has many proven advantages, including small size, light weight, immunity to electromagnetic interference, low cost for single-use application and capability of integration of excitation light source and acoustic wave receiver.

This dissertation demonstrates an open cavity, micro-cantilever based fiber optic Fabry-Perot interferometer (FPI) hydrophone. A fused silica micro-cantilever …

Synthesis And Characterization Of Glycidyl Methacrylate-Based Graft Copolymer Functional Interfaces, Nikolay Brordinov

All Dissertations

Modification of materials properties such as surface energy, wettability, ability to absorb, contain or release specific type of chemicals enables practical application of these materials in various scientific and engineering set-ups. However, designing protocol that could be easily adapted for different situations and simultaneously unlock multiple variations of the resulting properties is a non-trivial task. This dissertation is devoted to application of glycidyl methacrylate-based graft copolymers for the purposes of surface modification, establishing fundamental trends and dependencies of this process and exploring the possible range of applications. These materials have extremely valuable property to undergo controllable post-synthetic modifications including surface …

Upland Cotton (Gossypium Hirsitum) Root Responses To Reniform Nematode (Rotylenchulus Reniformis) Infection, Wei Li

All Dissertations

Reniform nematode (Rotylenchulus reniformis) is a semi-endoparasitic nematode that causes yield losses in numerous crops, including upland cotton (Gossypium hirsutum). Resistant cotton varieties are urgently needed, but the molecular basis of nematode-host plant interactions is poorly understood. To document plant responses to reniform nematode infection in upland cotton roots, we set up a split-root growth system to collect tissues from infected and uninfected portions of the same root system. A 12-day time course of histology and gene expression of infected roots were generated in Chapter 1. Results suggest reniform nematode infection induced protein synthesis and transport, …

Assessing The Cultural Effects Of Neoliberalism On Empathy, Melissa May Racho

Dissertations

The current study examined the effect of neoliberalism on the expression of empathy among 40 undergraduate college students. Participants were divided across three groups: two treatment groups who were exposed to either a neoliberal or critical primer and a control group with no exposure to a primer. Individuals were randomly assigned to each condition prior to completing three empathy measures: The Empathic Concern and Perspective Taking subscales of the Interpersonal Reactivity Index, and the Reading the Mind in the Eyes Test. Mean scores were analyzed using multivariate analysis of variance (MANOVA) and revealed no significant differences between groups, indicating the …

The Effects Of Dopamine On Frequency Dependent Short Term Synaptic Plasticity: A Comparison Of Layer Contributions And Rhythmic Dynamics, Jonna Marie Jackson

Dissertations

Executive functions (e.g. working memory [WM]) are known to be mediated by prefrontal cortical areas of the human brain which share homology with mouse medial prefrontal cortex (mPFC). Furthermore, it is well established that optimal dopaminergic input is required for proper WM function in the PFC. While it is well established that the mPFC receives inputs from several different brain areas, impinging on different compartmental regions of cells, it remains unknown how layer V pyramidal cells, the major output cells of the mPFC, integrate this information. Additionally, it remains unknown how dopamine modulates this integration by way of separate afferents …

Factors Associated To Teacher Longevity In A Title I Elementary School: A Qualitative Narrative Inquiry Study, Richard Devney

Department of Educational Administration: Dissertations, Theses, and Student Research

The potential of our nation is hinged upon the strength of our educational system across all 50 states. Public education is the backbone of our country and continues to be a spotlight of focus for many. In 1983, President Ronald Regan shared the “Nation at Risk” report, based on the findings from the National Commission on Excellence in Education. The report claimed a level of mediocrity permeated the public education system and children were lagging behind compared to children in other first world countries. This landmark report put a level of accountability on school officials to create higher performing students …

Latinx Students In Stem Education Research: A Crt And Latcrit Analysis Of Nsf Funded Projects, Hortencia Lara

Department of Educational Administration: Dissertations, Theses, and Student Research

This thesis used Critical Race Theory (CRT) and Latino Critical Race Theory (LatCrit) to conduct an in-depth analysis of whether literature funded through the use of National Science Foundation (NSF) research awards perpetuates race, racism, or other interacting systems of oppression in the research or if the investigators resisted inequalities against Latinx students in STEM research. This thesis examined how the investigators of twenty NSF-funded articles examined the experiences of Latinx students in STEM. From a CRT and LatCrit lens I analyze articles to see if and in what ways researchers are complicit with oppression and which ways they resist. …

Examination Of Collaborative Efforts Of Central District To Achieve Success Of The Community Health Improvement Plan, Liene Topko

Capstone Experience: Master of Public Health

This was a quality improvement project that helped Central District Health Department (CDHD) work more closely with their community partners to identify the activities and programs that are currently in place to address priority health needs that were included in the 2016 Comprehensive Community Health Needs Assessment (CHNA). The purpose of this project is to identify the specific activities and programs that are being implemented by the health department and community partners in relation to the priority needs. These activities and programs were identified by conducting a survey of the major community partners. The findings from the survey helped to …

Evaluation Of Adult Mental Health First Aid: Findings From 2015-2017 Post-Course Surveys, Gina Schwieger

Capstone Experience: Master of Public Health

Abstract

Introduction: Lack of knowledge about mental health, lack of access to mental health care, and stigma create substantial barriers to the overall United States adult population from seeking or helping individuals with behavioral health issues. This study evaluated Adult Mental Health First Aid (MHFA) courses, an evidence-based program for increasing mental health knowledge, decreasing stigma, and increasing the community’s confidence in helping and frequency of referring people in need.

Methods: This retrospective evaluation of 2015-2017 Adult MHFA courses, utilizes qualitative and quantitative data from post-course surveys completed by course participants. Quantitative data was measured using a five-point Likert Scale. …

Survey Of Indoor Air Quality In Residences Of Wic Participants In Lincoln, Nebraska, Eun Hee Kwon

Capstone Experience: Master of Public Health

Since 1992, Women, Infants, and Children at the Lincoln Lancaster County Health Department has provided supplemental food benefits, nutrition information, breastfeeding support, immunization referrals, and dental services to support low-income families and their children. However, statewide, the retention rate has been dropping since 2009, and if this continues, it can result in adverse health outcomes. As part of the Service Learning activities, this study aims to evaluate current services on clients’ experiences and improve their satisfaction by conducting the WIC participant-centered services survey. This cost-effective intervention program has been working successfully, however it does not provide the information of environmental …

Three-Dimensional Context Rather Than Nls Amino Acid Sequence Determines Importin Α Subtype Specificity For Rcc1., Rajeshwer S. Sankhala, Ravi K. Lokareddy, Salma Begum, Ruth A. Pumroy, Richard E. Gillilan, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

Active nuclear import of Ran exchange factor RCC1 is mediated by importin α3. This pathway is essential to generate a gradient of RanGTP on chromatin that directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Here we identify the mechanisms of importin α3 selectivity for RCC1. We find this isoform binds RCC1 with one order of magnitude higher affinity than the generic importin α1, although the two isoforms share an identical NLS-binding groove. Importin α3 uses its greater conformational flexibility to wedge the RCC1 β-propeller flanking the NLS against its lateral surface, preventing steric clashes with its Armadillo-core. Removing …

The Structure And C=C Vibrational Frequencies Of The All- Trans Polyenes C2nh2n+2(N=2-15), C2nh2n(Me)2(N=2-13), And C2nh2n(Tert-Butyl)2(N=2-5): Computational Results, George R. De Maré, Niny Z. Rao, Charles W. Block

Department of Biochemistry and Molecular Biology Faculty Papers

Carbon-carbon bond lengths and C=C vibrational frequencies are reported for the linear, all-trans unsubstituted C2nH2n+2 (n=2-15), methyl capped C2nH2nMe2 (n=2-13), and tert-butyl capped C2nH2n(tert-butyl)2 (n=2-5) polyenes (C2h) calculated at the B3LYP/6-311++G(d,p) level. The C=C/C-C bond length alternation remains evident at this level for the unsubstituted and methyl capped polyenes as the chain length increases; the center-most difference in the length of the C-C/C=C bonds is ~0.06 Å for C30H32 and C26H26Me2. The Ag, in-phase, harmonic C=C Raman frequency for the unsubstituted polyenes decreases from 1699.2 cm-1 (n = 2) to 1528.9 cm-1 (n=15); the anharmonic frequency decreases from 1651.5 cm-1 …

Synergy Of Two Low-Affinity Nlss Determines The High Avidity Of Influenza A Virus Nucleoprotein Np For Human Importin Α Isoforms., Wei Wu, Rajeshwer S. Sankhala, Tyler J Florio, Lixin Zhou, Nhan L.T. Nguyen, Ravi K. Lokareddy, Gino Cingolani, Nelly Panté

Department of Biochemistry and Molecular Biology Faculty Papers

The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. Through site-directed mutagenesis and functional analysis, we found that NLS2 is also monopartite and is indispensable for viral infection. Atomic structures of importin α bound to two variants of NLS2 revealed NLS2 primarily binds the major-NLS binding site of importin α, unlike NLS1 that associates …

Astaxanthin Attenuates Mptp Induced Neurotoxicity And Modulates Cognitive Function In Aged Mice, Beth Grimmig

USF Tampa Graduate Theses and Dissertations

Parkinson’s disease is the second common neurodegenerative disease and is most frequently diagnosed in individuals over 60. There are no available medications that can prevent or restore the loss of neurons that correspond to motor impairments in patients. Identifying novel therapeutic compounds that are capable of slowing and reversing the extensive neurodegeneration that occurs in PD remains an important goal of the field. While basic research has identified potential therapeutic agents, studies often use young model organisms to demonstrate efficacy of the target compound. This approach ignores the impact of the aged CNS on the disease process, and likely contributes …

Anti-Proliferative Activity Of Crotalaria Pallida Aiton On Mcf-7 Breast Cancer Cells, Erladys M. Rumondor, Moelyono Moektiwardoyo, Melisa I. Barliana

Pharmacology and Clinical Pharmacy Research

Crotalaria pallida Aiton (C. pallida Aiton) is empirically used as dietary supplement to treat cancer by the people of North Sulawesi. However, its scientific pharmacology activity has not been explored yet. Therefore, this study was conducted to evaluate anti-proliferative activity of C. pallida Aiton on MCF-7 breast cancer cells. The extraction of leaves and seeds were performed using ethanol, ethyl acetate, n-hexane, and water. Phytochemical screening was then performed to identify secondary metabolites in this extract. Anti-proliferative activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated that ethyl acetate fraction of C. pallida Aiton has the lowest IC50 …

Reduction Of Escherichia Coli Surrogates For Shiga Toxin-Producing Escherichia Coli On Beef Cuts By Dipping In Lactic Or Peroxyacetic Acid For Short Or Extended Times And The Effect On Ground Beef Quality, Ashley Rae Mccoy

Department of Animal Science: Dissertations, Theses, and Student Research

Shiga Toxin-producing Escherichia coli (STEC) are adulterants in ground beef. Antimicrobial interventions reduce STEC, but effectiveness may depend on application time and the impact on ground beef quality.

Objectives were to evaluate reductions in rifampicin-resistant E. coli (E. coli^RIF) on beef clod surfaces, and the effect on ground beef quality. Beef clods were sectioned and dipped in 4.5% lactic acid (LA) or 380 ppm peroxyacetic acid (PA) for 15 or 180 s. E. coli^RIF were inoculated (~5.3 log CFU/cm2) onto fat or lean tissue surfaces. Non- inoculated treatments were ground into ~454 g …

A Study Of Perceived Leadership Styles As It Affects The Decision-Making Process Employed By Eleven Catholic School Principals In South Texas, Mary Olivia Agbakoba

Theses and Dissertations

Catholic School principals play an important role in the development of students' spiritual, social, and academic wellbeing. Consequently, in order to improve students' spiritual, social, and academic skill, it is vital to study the perceived leadership styles and decision-making of Catholic School Principals. Research questions include: "Is there a relationship between Catholic School Principals and teachers' perceptions on principals' leadership styles?", and "Is there an association between decision type and decision-making style used?" 11 Catholic School Principals and 179 Catholic School Teachers completed a paper and pencil survey that assessed the perceived leadership styles of principals. Data was analyzed using …

Phylogeography Of An Estuarian Calanoid Copepod; Acartia Tonsa In The Texas Gulf Of Mexico, Nicole J. Figueroa

Theses and Dissertations

The calanoid copepod, Acartia tonsa is one of the most abundant and well-studied estuarian species. However, the idea that this cosmopolitan species has unrestricted dispersal and high gene flow has been challenged. In this study, a more holistic picture of the phylogeography of A. tonsa was developed using the mitochondrial gene cytochrome oxidase one (mtCOI). Multiple new lineages were found in the Texas Gulf of Mexico that are basal to Northeastern Atlantic lineages. Connectivity was also observed between Brazil and the Texas Gulf of Mexico. The revised phylogeny shows a clear pattern of cryptic speciation as the species made a …

Hydrodesulfurization Of Dibenzothiophene Using Lanthanum Doped Comos2, Carolina Valdes Bracamontes

Theses and Dissertations

Hydrodesulfurization (HDS) is a technique used in petrochemical industry for the removal of sulfur from crude oil. Using dibenzothiophene (DBT) as a target molecule and CoMoS2 catalysts doped with various concentrations of lanthanum were studied for catalytic activity in the removal of DBT from solution. Catalysts were synthesized using ammonium tetrathiomolydate, cobalt chloride and lanthanum nitrate under a solvothermal decomposition in decalin at 350 °C with H2 gas at 160 psi. To evaluate decomposition effects, a second set of catalyst precursors were decomposed in a tube furnace at 450 °C under a constant flow of 10%/90% H2/Ar. Characterizations of the …

Effective Parental Cooperation And Communication As Protective Factors For Adult Offspring Of Divorce, Damla Til Ogut

Theses and Dissertations

Research and society have focused attention on how divorce affects family dynamics for decades. The clinical literature points to significant discrepancies in psychological variables between offspring from divorced versus intact families, suggesting that divorce contributes to negative psychosocial outcomes. Accordingly, investigations have revealed the adverse effects of co-parental conflict and antagonistic communication on divorce offspring’s psychological outcomes. Although parental cooperation and communication are suggested to serve as protective factors, more research is needed, given the current literature’s reliance on measures of psychopathology, yet making conclusions on psychological wellness. Among 244 college students, results indicated that divorce offspring reported lower levels …