Digital Commons Network^™

Effects Of An Unusual Poison Identify A Lifespan Role For Topoisomerase 2 In Saccharomyces Cerevisiae, Gregory Tombline, Jonathan Millen, Bogdan Polevoda, Matan Rapaport, Bonnie Baxter, Michael Van Meter, Matthew Gilbertson, Joe Madrey, Gary A. Piazza, Lynn Rasmussen, Kirster Wennerberg, E. Lucile White, John L. Nitiss, David S. Goldfarb

Biology Faculty/Staff Publications

A progressive loss of genome maintenance has been implicated as both a cause and consequence of aging. Here we present evidence supporting the hypothesis that an age-associated decay in genome maintenance promotes aging in Saccharomyces cerevisiae (yeast) due to an inability to sense or repair DNA damage by topoisomerase 2 (yTop2). We describe the characterization of LS1, identified in a high throughput screen for small molecules that shorten the replicative lifespan of yeast. LS1 accelerates aging without affecting proliferative growth or viability. Genetic and biochemical criteria reveal LS1 to be a weak Top2 poison. Top2 poisons induce the accumulation of …

Peixoto Studies, Teaches The Powerhouses Of Life., Aldemaro Romero Jr.

Publications and Research

Sometimes great things come from small ones. Among the smallest structures we find in almost all cells are the mitochondria. Not only are they very small (about 0.001of an inch), but they are also very numerous (some of our cells have more than 2,000 of them each). And their role in life is enormous: they generate the energy molecules we use to make our bodies work.

Pablo Peixoto studies these microscopic structures. Born in Brasilia, the capital of Brazil, he became interested in science early in life. “I was one of those annoying kids who always wanted to know the …

Teaching And Learning Mathematics In The Ar/Vr Environment, Alexander Vaninsky

Publications and Research

This presentation discusses teaching and learning mathematics in augmented (AR) or virtual (VR) reality created by a combination of goggles and earphones. It claims that interactive learning in such an environment is more attractive and efficient. It increases motivation and interest in the subject matter. The approach is underlain by the findings of educational neuroscience considering the learning process as the formation of domains in the brain forming mathematics knowledge centers. The teaching process provides sensory excitation and establishes connections among these and other domains. Hardware and software are available in the market. The suggested approach allows for practical implementation …

Myogenic Progenitor Cells Control Extracellular Matrix Production By Fibroblasts During Skeletal Muscle Hypertrophy, Christopher S. Fry, Tyler J. Kirby, Kate Kosmac, John J. Mccarthy, Charlotte A. Peterson

Physiology Faculty Publications

Satellite cells, the predominant stem cell population in adult skeletal muscle, are activated in response to hypertrophic stimuli and give rise to myogenic progenitor cells (MPCs) within the extracellular matrix (ECM) that surrounds myofibers. This ECM is composed largely of collagens secreted by interstitial fibrogenic cells, which influence satellite cell activity and muscle repair during hypertrophy and aging. Here we show that MPCs interact with interstitial fibrogenic cells to ensure proper ECM deposition and optimal muscle remodeling in response to hypertrophic stimuli. MPC-dependent ECM remodeling during the first week of a growth stimulus is sufficient to ensure long-term myofiber hypertrophy. …

Development, Validation, And Clinical Application Of A Numerical Model For Pulse Wave Velocity Propagation In A Cardiovascular System With Application To Noninvasive Blood Pressure Measurements, Jeffrey S. Lillie

Theses

High blood pressure blood pressure is an important risk factor for cardiovascular disease and affects almost one-third of the U.S. adult population. Historical cuff-less non-invasive techniques used to monitor blood pressure are not accurate and highlight the need for first principal models. The first model is a one-dimensional model for pulse wave velocity (PWV) propagation in compliant arteries that accounts for nonlinear fluids in a linear elastic thin walled vessel. The results indicate an inverse quadratic relationship (R^2=.99) between ejection time and PWV, with ejection time dominating the PWV shifts (12%). The second model predicts the general relationship between PWV …

Transgender Care, Nyasha George, Md, Pgy 3

Department of Family & Community Medicine Presentations and Grand Rounds

Outline:

Objectives

Review of trans epidemiology

Healthcare and the Transcommunity

Cases 1&2

• Taking a transgender history
• CSH indications

Case 3

• Mental Health and Transpersons

Case 4

• Cardiovascular Risks and Estrogen in Transwomen
• Provider Resources

Objectives

My goals for you at the end of this lecture:

Comfort in determining which transgender patients meet criteria for initiating CSH

Appreciation of the overlap between transgender identity and psychiatric disease as mediated primarily by trauma

Improved self efficacy in weighing risks/benefits of CSH therapy

Familiarity with resources available to help support primary care providers take care of transgender patients.

Presentation: 58:36

Singer, Wittgenstein, And Morally Motivating Examples, Ramona Ilea

Between the Species

Peter Singer’s Animal Liberation played a pivotal role in the animal rights movement and the foundation of modern animal ethics. Using an analysis inspired by Wittgenstein’s remarks on ethics, I will analyze the way in which Singer’s book is structured in order to understand why it succeeds in providing people with the moral motivation to change the way they live. I will argue that the success of Animal Liberation is in large part due to the detailed, carefully chosen, emotionally rich examples and the unusual way in which these examples are juxtaposed, structured, and presented. Understanding how examples can have …

Understanding And Overcoming Antifolate Resistance In Methicillin-Resistant Staphylococcus Aureus, Stephanie M. Reeve

Doctoral Dissertations

Resistance to every class of antibiotics has been identified clinically. The rapid development and spread of resistance is responsible for the diminished lifetime of many once efficacious therapeutics. Trimethoprim (TMP), a clinically important dihydrofolate reductase (DHFR) inhibitor, remains a first line treatment for Staphylococcus aureus infections but its continued use is threatened by the emergence of resistance. Identifying and understanding the mechanisms of trimethoprim resistance is crucial for the development for novel antibiotics to overcome the contemporary clinical resistances. Trimethoprim resistance is conferred through both the acquisition of mutations in the endogenous DHFR enzyme and of innately resistant enzymes. Guided …

Biophysics Of Dendritic Spine Morphogenesis, Olena Marchenko

Doctoral Dissertations

Dendritic spines receive the majority of synaptic inputs in the mammalian central nervous system and constitute the foundation for a healthy nervous system. Cognitive and motor delays, and other symptoms of impaired neurodevelopment are associated with abnormal dendritic spine shape and densities. Understanding of spine stability and spine formation in neurodevelopmental conditions remains elusive. Spine formation is determined by the stability of its transient precursor: the dendritic filopodium. First, we aim to understand dendritic filopodium motility and stability mechanism that underlies its transition into a spine. We hypothesized that mechanical feedback among the actin retrograde flow, myosin activity, and substrate …

Ck2—An Emerging Target For Neurological And Psychiatric Disorders, Julia Castello, Andre Ragnauth, Eitan Friedman, Heike Rebholz

Publications and Research

Protein kinase CK2 has received a surge of attention in recent years due to the evidence of its overexpression in a variety of solid tumors and multiple myelomas as well as its participation in cell survival pathways. CK2 is also upregulated in the most prevalent and aggressive cancer of brain tissue, glioblastoma multiforme, and in preclinical models, pharmacological inhibition of the kinase has proven successful in reducing tumor size and animal mortality. CK2 is highly expressed in the mammalian brain and has many bona fide substrates that are crucial in neuronal or glial homeostasis and signaling processes across synapses. Full …

The Database Of The Predicts (Projecting Responses Of Ecological Diversity In Changing Terrestrial Systems) Project, Lawrence N. Hudson, Tim Newbold, Sara Contu, Samantha L.L. Hill, Igor Lysenko, Palma A De, Helen R.P. Phillips, Tamera I. Alhusseini, Felicity E. Bedford, Dominic J. Bennett, Hollie Booth, Victoria J. Burton, Charlotte W.T. Chng, Argyrios Choimes, David L.P. Correia, Julie Day, Susy Echeverría-Londoño, Susan R. Emerson, Di Gao, Morgan Garon, Michelle L.K. Harrison, Daniel J. Ingram, M Jung, Victoria Kemp, Lucinda Kirkpatrick, Callum D. Martin, Yuan Pan, Gwilym D. Pask-Hale, Edwin L. Pynegar, An Robinson

School of Biological and Marine Sciences

No abstract provided.

Space, Land, Territory, And The Study Of The Bible, Stephen C. Russell

Publications and Research

In this brief volume, written for professional biblical scholars and graduate students being trained in biblical studies, Stephen C. Russell introduces the reader to the interdisciplinary study of space and its related concepts, including land, territory, border, frontier, nature, scale, spatial flows, and rhythm. He offers a synopsis of eight approaches to the study of space that have been influential in the humanities and social sciences in recent decades—sacred, legal, political, economic, ecological, visual, social, and urban approaches. He pays special attention to Henri Lefebvre’s treatment of social space as a social product. The volume also briefly notes some of …

Campus Vol Ix N 4, Mike Cook, Bruce Mcintosh, Joen Pritchard, Prentice Stout, John Hodges, C. Tuttle, George M. Fenberg, Midge Greenlee, John Miller, Beth Hodge, Tip Rasor, Shaw Kull

Campus

Howard Studio. Cover. Picture. 1.

Anonymous. Untitled. Prose. 4.

Cook, Mike. "misty Mood". Prose. 5.

McIntosh, Bruce. "Misty Mood". Picture. 5.

Pritchard, Joen. "In the Good 'Ole Summertime...". Prose. 6.

Stout, Prentice. "In the Good 'Ole Summertime...". Picture. 6.

Hodges, John. "How to Choose a Roommate". Prose. 7.

Tuttle, C. Untitled. Cartoon. 7.

Fenberg, Geo. M. "Students---". Prose. 3.

Wood and Knapp. "Jokes From the Jazz Age as Flips From Flappers". Prose. 8.

Greenlee, Midge. "Nine to Five". Prose. 10.

Anonymous. "Mr. and Miss Campus-1954". Picture. 11.

Anonymous. "Well Shut My Mouth!". Prose. 14.

Tuttle, C. Untitled. Cartoon. 14.; Miller, …

Conceptual Framework Of Community Based Location Specific Services For Improved Service Delivery, Folasade Dahunsi

The African Journal of Information Systems

Community Based Location Service (CBLS) is the provision of information about services based on the geographical location of subscriber’s mobile phone towards the development of the community they are in. With the increase in mobile phones penetration, citizens can participate actively in providing relevant information to assist in the provision of services and infrastructure in the country through their mobile devices. This paper proposes a framework that will improve services and provide better infrastructure to developing countries. It hinges on four major technologies: location-based information, crowdsourcing, mobile communication devices and volunteerism. This framework takes into consideration the importance of motivating …

Oxygen Concentration Modulates Cardiac Stem Cell Proliferation And Migration, Michael A. Bellio

Open Access Dissertations

Cardiac stem cells (CSCs) are being evaluated for their efficacy in the treatment of heart failure. However, numerous factors impair the exogenously delivered cells’ regenerative capabilities. Hypoxia is one stress that contributes to inadequate tissue repair. Here, we tested the hypothesis that hypoxia impairs cell proliferation, survival, and migration of human CSCs relative to physiological and room air oxygen concentrations. Human endomyocardial biopsy-derived CSCs were isolated, selected for c-Kit expression, and expanded in vitro at room air (21% O2). To assess the effect on proliferation, survival, and migration, CSCs were transferred to physiologic (5%) or hypoxic (0.5%) O2 concentrations. Physiologic …

01-04-2017 Swosu Bestows Annual Emeriti Honors, Southwestern Oklahoma State University

THE BARK ARCHIVE 2017

Four former Southwestern Oklahoma State University faculty were honored recently by the SWOSU Alumni Association during a reception held in the Skyview Suite of the Pioneer Cellular Events Center on the Weatherford campus.

Out In "The Numbers": Youth And Gang Violence Initiatives And Uneven Development In Portland's Periphery, Dirk Kinsey

Dissertations and Theses

Incidence of youth and gang violence in the Portland, Oregon metro area has increased dramatically over the past five years. This violence has recently become more spatially diffuse, shifting outwards from gentrified, inner city neighborhoods, towards the city's periphery. These incidents exist within the context of a shifting regional political economy, characterized by a process of gentrification associated displacement and growing, and distinctly racialized and spatialized, inequalities. While gang researchers have long argued a corollary between the emergence of gangs and economically and culturally polarized urban landscapes, the ongoing suburbanization of poverty in American cities suggests a new landscape of …

Ehlers-Danlos Syndrome Hypermobility Type Is Associated With Rheumatic Diseases, Kyla R. Rodgers, Jiang Gui, Mary Beth P. Dinulos, Richard C. Chou

Dartmouth Scholarship

We retrospectively analyzed electronic medical records of patients with Ehlers-Danlos Syndrome hypermobility type (HEDS), including demographic information, workup, rheumatological diagnoses in order to determine its association with rheumatological conditions. HEDS Patients were stratified according to level of workup received (no additional work (physical exam only) = NWU, limited workup = LWU, comprehensive workup = CWU)). HEDS patients were predominantly female (21:4, F:M). The percentage of patients with at least one rheumatological condition was significantly correlated with level of workup (NWU, 9.2%; LWU, 33.3%, CWU, 67.1%; p-value < 0.0001). The HLA-B27 antigen was more prevalent (p-value < 2.2 × 10 –8 ) in the CWU HEDS patients (23.9%) than in the general population of the United States (6.1%). HEDS with CWU were associated with more rheumatological conditions (i.e. psoriasis, ankylosing spondylitis, rheumatoid arthritis, fibromyalgia) than those with NWU or LWU. In conclusion, HEDS is associated with complicated rheumatological conditions, which are uncovered by comprehensive workup. These conditions require different clinical management strategies than HEDS, and left untreated could contribute to the pain or even physical disability (i.e. joint erosions) in HEDS patients. While the mechanisms underlying these associations are unknown, it is important that all HEDS patients receive adequate workup to ensure a complete clinical understanding for the best care strategy possible.

Idiosyncratic Drug-Induced Liver Injury (Idili): Potential Mechanisms And Predictive Assays, Alexander D. Roth, Moo-Yeal Lee

Chemical & Biomedical Engineering Faculty Publications

Idiosyncratic drug-induced liver injury (IDILI) is a significant source of drug recall and acute liver failure (ALF) in the United States. While current drug development processes emphasize general toxicity and drug metabolizing enzyme- (DME-) mediated toxicity, it has been challenging to develop comprehensive models for assessing complete idiosyncratic potential. In this review, we describe the enzymes and proteins that contain polymorphisms believed to contribute to IDILI, including ones that affect phase I and phase II metabolism, antioxidant enzymes, drug transporters, inflammation, and human leukocyte antigen (HLA). We then describe the various assays that have been developed to detect individual reactions …

Palladium-Catalyzed Intramolecular Cross-Dehydrogenative Coupling: Synthesis Of Fused Imidazo[1,2‑A]Pyrimidines And Pyrazolo[1,5‑A]Pyrimidines, Siva K. Reddy Kotla, Jaya Kishore Vandavasi, Jeh-Jeh Wang, Keykavous Parang, Rakesh Tiwari

Pharmacy Faculty Articles and Research

A palladium-catalyzed intramolecular dehydrogenative coupling reaction was developed for the synthesis of fused imidazo[1,2-a]pyrimidines and pyrazolo[1,5-a]- pyrimidines. The developed protocol provides a practical approach for the synthesis of biologically important substituted pyrimidines from easily available substrates, with a broad substrate scope under mild reaction conditions.

Spring Temperature Variability Over Turkey Since 1800 Ce Reconstructed From A Broad Network Of Tree-Ring Data, Nesibe Köse, H. Tuncay Güner, Grant L. Harley, Joel Guiot

Faculty Publications

The meteorological observational period in Turkey, which starts ca. 1930 CE, is too short for understanding long-term climatic variability. Tree rings have been used intensively as proxy records to understand summer precipitation history of the region, primarily because they have a dominant precipitation signal. Yet, the historical context of temperature variability is unclear. Here, we used higher-order principle components of a network of 23 tree-ring chronologies to provide a high-resolution spring (March–April) temperature reconstruction over Turkey during the period 1800– 2002. The reconstruction model accounted for 67 % (Adj. R 2 = 0.64, p < 0.0001) of the instrumental temperature variance over the full calibration period (1930–2002). The reconstruction is punctuated by a temperature increase during the 20th century; yet extreme cold and warm events during the 19th century seem to eclipse conditions during the 20th century. We found significant correlations between our March–April spring temperature reconstruction and existing gridded spring temperature reconstructions for Europe over Turkey and southeastern Europe. Moreover, the precipitation signal obtained from the tree-ring network (first principle component) showed highly significant correlations with gridded summer drought index reconstruction over Turkey and Mediterranean countries. Our results showed that, beside the dominant precipitation signal, a temperature signal can be extracted from tree-ring series and they can be useful proxies in reconstructing past temperature variability.

Inhibition Of Mammalian Glycoprotein Ykl-40 Identification Of The Physiological Ligand, Abhishek A. Kognole, Christina M. Payne

Chemical and Materials Engineering Faculty Publications

YKL-40 is a mammalian glycoprotein associated with progression, severity, and prognosis of chronic inflammatory diseases and a multitude of cancers. Despite this well documented association, identification of the lectin′s physiological ligand and, accordingly, biological function has proven experimentally difficult. YKL-40 has been shown to bind chito-oligosaccharides; however, the production of chitin by the human body has not yet been documented. Possible alternative ligands include proteoglycans, polysaccharides, and fibers like collagen, all of which makeup the extracellular matrix. It is likely that YKL-40 is interacting with these alternative polysaccharides or proteins within the body, extending its function to cell biological roles …

Evaluating Simplistic Methods To Understand Current Distributions And Forecast Distribution Changes Under Climate Change Scenarios: An Example With Coypu (Myocastor Coypus), Catherine S. Jarnevich, Nicholas E. Young, Trevor R. Sheffels, Jacoby Carter, Mark D. Sytsma, Colin Talbert

Environmental Science and Management Faculty Publications and Presentations

Invasive species provide a unique opportunity to evaluate factors controlling biogeographic distributions; we can consider introduction success as an experiment testing suitability of environmental conditions. Predicting potential distributions of spreading species is not easy, and forecasting potential distributions with changing climate is even more difficult. Using the globally invasive coypu (Myocastor coypus [Molina, 1782]), we evaluate and compare the utility of a simplistic ecophysiological based model and a correlative model to predict current and future distribution. The ecophysiological model was based on winter temperature relationships with nutria survival. We developed correlative statistical models using the Software for Assisted Habitat Modeling …

Hyaline Fibromatosis Syndrome: A Novel Mutation And Recurrent Founder Mutation In The Cmg2/Antxr2 Gene., Leila Youssefian, Hassan Vahidnezhad, Yahya Aghighi, Vahid Ziaee, Sirous Zeinali, Maryam Abiri, Jouni Uitto

Department of Dermatology and Cutaneous Biology Faculty Papers

No abstract provided.

Effect Of Trehalose As An Additive To Dimethyl Sulfoxide Solutions On Ice Formation, Cellular Viability, And Metabolism., Jason Solocinski, Quinn Osgood, Mian Wang, Aaron Connolly, Michael A. Menze, Nilay Chakraborty

Faculty and Staff Scholarship

Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (−196 °C). The organic solvent dimethyl sulfoxide (Me₂SO) is one of the most commonly utilized CPAs and has been used with various levels of success depending on the type of cells. In recent years, to improve cryogenic outcomes, the non-reducing disaccharide trehalose has been used as an additive to Me₂SO-based freezing solutions. Trehalose is a naturally occurring non-toxic compound found in bacteria, …

Sperm Pairing And Measures Of Efficiency In Planar Swimming Models, Paul Cripe, Owen Richfield, Julie Simons

Spora: A Journal of Biomathematics

Sperm of certain species engage in cooperative swimming behaviors which result in differences in velocity and efficiency of swimming, as well as ability to effectively fertilize the egg. In particular, Monodelphis domestica is a species of opossum whose sperm often swim cooperatively as a pair, with heads fused together. In order to understand the empirical effects of cooperative swimming behaviors, we propose a simple preferred-curvature-based model to model individual and paired sperm using the method of regularized Stokeslets to model the viscous fluid environment. The effects of swimming freely versus paired swimming, phase relationship, and the angle at which sperm …

Parts Of The Whole: Error Estimation For Science Students, Dorothy Wallace

Numeracy

It is important for science students to understand not only how to estimate error sizes in measurement data, but also to see how these errors contribute to errors in conclusions they may make about the data. Relatively small errors in measurement, errors in assumptions, and roundoff errors in computation may result in large error bounds on computed quantities of interest. In this column, we look closely at a standard method for measuring the volume of cancer tumor xenografts to see how small errors in each of these three factors may contribute to relatively large observed errors in recorded tumor volumes.

Quantitative Literacy For The Future Flourishing Of Our Students: A Guiding Aim For Mathematics Education, Samuel L. Tunstall

Numeracy

In this essay, I examine the extent to which mathematics education and education for quantitative literacy support students’ present and future flourishing, a concept that entails realizing objective goods in a life lived from the inside. This perspective requires disentangling philosophical assumptions about the aims of mathematics education, which—in the context of flourishing—I take to be a hybrid of those that have informed curricular discussions over the past two centuries. In the process, I problematize ("make strange") many of the common reasons given for students learning mathematics, including: learning it for one’s career, for one’s logical reasoning skills, or …

A Twenty-Year Look At “Computational Geology,” An Evolving, In-Discipline Course In Quantitative Literacy At The University Of South Florida, Victor J. Ricchezza, H. L. Vacher

Numeracy

Since 1996, the Geology (GLY) program at the USF has offered “Computational Geology” as part of its commitment to prepare undergraduate majors for the quantitative aspects of their field. The course focuses on geological-mathematical problem solving. Over its twenty years, the course has evolved from a GATC (geometry-algebra-trigonometry-calculus) in-discipline capstone to a quantitative literacy (QL) course taught within a natural science major. With the formation of the new School of Geosciences in 2013, the merging departments re-examined their various curricular programs. An online survey of the Geology Alumni Society found that “express quantitative evidence in support of an argument” was …

Campus Vol Vii N 4, Pete Hawk, Lyn Martin

Campus

Howard Studio. "Camile Champlin, Freshman". Picture. 4.

Hawk, Pete. "It's Just a Game". Prose. 6.

Anonymous. "How To Snow Finals". Prose. 8.

Martin, Lyn. "Keep Granville Clean!". Prose. 9.

Anonymous. "Last Will and Testament of the Senior Class". Prose. 10.

Anonymous. Untitled. Poem. 13.

Anonymous. Untitled. Prose. 13.