Digital Commons Network^™

Does Plasticity In Thermal Tolerance Trade Off With Inherent Tolerance? The Influence Of Setal Tracheal Gills On Thermal Tolerance And Its Plasticity In A Group Of European Diving Beetles., W. C.E.P. Verberk, P. Calosi, J. I. Spicer, S. Kehl, D. T. Bilton

School of Biological and Marine Sciences

In the face of global warming, both the absolute thermal tolerance of an ectotherms, and its ability to shift its tolerance level via acclimation, are thought to be fundamentally important. Understanding the links between tolerance and its plasticity is therefore critical to accurately predict vulnerability to warming. Previous studies in a number of ectotherm taxa suggest trade-offs in the evolution of thermal tolerance and its plasticity, something which does not, however, apply to Deronectes diving beetles, where these traits are instead positively correlated. Here we revisit the relationship between thermal tolerance and plasticity in these beetles, paying attention to a …

Expression Of Wnt-Signaling Pathway Genes And Their Associations With Mirnas In Colorectal Cancer, Martha L. Slattery, Lila E. Mullany, Lori C. Sakoda, Wade S. Samowitz, Roger K. Wolff, John R. Stevens, Jennifer S. Herrick

Mathematics and Statistics Faculty Publications

The Wnt-signaling pathway functions in regulating cell growth and thus is involved in the carcinogenic process of several cancers, including colorectal cancer. We tested the hypothesis that multiple genes in this signaling pathway are dysregulated and that miRNAs are associated with these dysregulated genes. We used data from 217 colorectal cancer (CRC) cases to evaluate differences in Wnt-signaling pathway gene expression between paired CRC and normal mucosa and identify miRNAs that are associated with these genes. Gene expression data from RNA-Seq and miRNA expression data from Agilent Human miRNA Microarray V19.0 were analyzed. We focused on genes most strongly associated …

Lived Experience In Patients With Recurrent Glioblastoma In Japan: A Narrative Study, Ai Chikada, Asako Takekuma Katsumata, Mariko Asase, Sayaka Takenouchi, Yoshiki Arakawa, Kazuko Nin

Asian/Pacific Island Nursing Journal

Glioblastoma (GBM) is well known to have one of the poorest prognoses among all cancers. Patients with GBM in progression-free survival (PFS) may be relatively stable and can often maintain their quality of life. Thus, PFS is a desirable goal. In Japan, the median PFS is 11 months. It is difficult to grasp a patient's thoughts and hopes when, after PFS, they are readmitted due to recurrence or acute deterioration. Therefore, this study aimed to describe the lived experience of illness in patients with recurrent GBM, focusing on PFS. We enrolled five patients into the study; however, only four patients …

Conversation And The Infinite Long Run, Anne Freadman

Criticism

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Seeing Witchcraft, Bernhard Udelhoven

Journal of Global Catholicism

When Christians in Zambia struggle with witchcraft, they also struggle with African cultural and religious concepts that deal with life’s ambiguities and that require discernment. It is not by working against the cultural and religious heritage, but by working with it, as far as possible, that the pastor can identify the broken relationships towards which many witchcraft discourses point. However, before we place the concepts of witchcraft into the realm of superstition (as are the trends of mission Christianity) or the demonic (as are the trends of charismatic Christianity), the Church has the duty to look at the concepts, stay …

Negative Impact Of Female Sex On Outcomes From Repetitive Mild Traumatic Brain Injury In Htau Mice Is Age Dependent: A Chronic Effects Of Neurotrauma Consortium Study, Scott A. Ferguson, Benoit C. Mouzon, Cillian Lynch, Carlyn Lungmus, Alexander Morin, Gogce Crynen, Benjamin Carper, Gayle Bieler, Elliott J. Mufson, William Stewart, Michael Mullan, Fiona Crawford

Translational Neuroscience

Traumatic brain injury (TBI) is a serious public health concern which strikes someone every 15 s on average in the US. Even mild TBI, which comprise as many as 75% of all TBI cases, carries long term consequences. The effects of age and sex on long term outcome from TBI is not fully understood, but due to the increased risk for neurodegenerative diseases after TBI it is important to understand how these factors influence the outcome from TBI. This study examined the neurobehavioral and neuropathological effects of age and sex on the outcome 15 days following repetitive mild traumatic brain …

Comparative Study Of Spinning Field Development In Two Species Of Araneophagic Spiders (Araneae, Mimetidae, Australomimetus), Mark A. Townley, Danilo Harms

Molecular, Cellular & Biomedical Sciences

External studies of spider spinning fields allow us to make inferences about internal silk gland biology, including what happens to silk glands when the spider molts. Such studies often focus on adults, but juveniles can provide additional insight on spinning apparatus development and character polarity. Here we document and describe spinning fields at all stadia in two species of pirate spider (Mimetidae: Australomimetus spinosus, A. djuka). Pirate spiders nest within the ecribellate orb-building spiders (Araneoidea), but are vagrant, araneophagic members that do not build prey-capture webs. Correspondingly, they lack aggregate and flagelliform silk glands (AG, FL), specialized for forming prey-capture …

Urologist Burnout: Frequency, Causes, And Potential Solutions To An Unspoken Entity, Julie Franc-Guimond, Brian Mcneil, Steven M. Schlossberg, Amanda C. North, Alp Sener

Anatomy and Cell Biology Publications

Physician burnout has been linked to decreased job performance, increased medical errors, interpersonal conflicts, and depression. Recent multispecialty studies suggest that urologists have higher rates (up to 63.6%) of burnout compared to physicians in other specialties; however, these reports were limited by low sample sizes.1 We aimed to evaluate the prevalence of urologist burnout, verify risk factors, and recommend preventative measures and solutions for colleagues at risk or suffering from burnout. Urologist burnout is a true entity that transcends level of training and nationality. Its roots appear to be deep-seated in our tireless efforts to strive for excellence in care …

Wind Is Nebraska’S Future, With Or Without The Clean Power Plan, Megan Pamperin

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

The Clean Power Plan was issued in October 2015. President Trump called for a review of the plan this March and on October 16^th, 2017 the EPA released a proposal to repeal it. The Clean Power Plan would have reduced Nebraska’s state emissions to 22,250,000 tons of carbon dioxide by 2024, down by about 5 million tons compared to 2012 emissions.¹ The new projection for 2020 without the plan is 34 million tons of CO₂ emitted.

Coal and other fossil fuel energy sources are the main culprits of these carbon emissions, and here in Nebraska coal …

Modifications To Commercial Printers To Enable Multi-Material Fabrication Of 3d Cellular Scaffolds, Lucas D. Albrecht

Theses - ALL

The scarcity of organs for patients that need transplants has led to exceedingly

lengthy waits for organ transplants for sick patients. Fabrication of tissues and organ

constructs in-vitro has potential to end the shortage, however many of the machines

used to create these tissues and organs are expensive, not easy to use, and do not

have any further practical applications. Bioprinting technology has the potential to

revolutionize the fabrication of biological constructs that can be used as in vitro model

tissues and vivo substitutes. Bioprinting is the process of using conventional 3D printing

methods and computer-aided-design (CAD) to create patient …

Cell Laden Hydrogel Microspheres Using 3d Printed Microfluidics, Sanika Nitin Suvarnapathaki

Theses - ALL

Cell laden hydrogel microspheres using 3D printed microfluidics

Abstract

By

Sanika Suvarnapathaki

Current tissue engineering therapies use macro-scale three dimensional (3D) scaffolds to treat tissue defects surgically. Uneven cell seeding and oxygen and media perfusion cause low cell viability in these macro-scale scaffolds. Microencapsulation, a technique of encapsulating cells in biocompatible polymers or hydrogels, has the potential to address these key issues, and therefore this technology has been used for numerous healthcare applications over the last two decades. Cell microencapsulation in hydrogels that mimic the tissue physiology and biochemistry has made it possible to use natural hydrogels like gelatin methacrylate …

Increased Striatal Vulnerability To 3-Nitropropionic Acid In Male, But Not Female, Mice Lacking Interleukin-1r1, Matthew Frederick Allen

Theses - ALL

Interleukin-1β (IL-1β), classically considered as a pro-inflammatory cytokine, has proven essential to cellular defense in nearly all tissues (Kaur et al., 2014, Ren and Torres, 2009). In brain, studies suggest that IL-1β has pleiotrophic effects. It acts as a neuromodulator, has been implicated in the pathogenic processes associated with a number of CNS diseases, but has also been shown to provide protection to the injured CNS. With respect to the latter, IL-1β signalling appears to mitigate pathology and motor symptoms in a mouse model of Huntington’s disease (HD), a progressive neurodegenerative condition that targets the striatum. Specifically, HD mice bred …

Perchlorate-Coupled Carbon Monoxide (Co) Oxidation: Evidence For A Plausible Microbe-Mediated Reaction In Martian Brines, Marisa R. Myers, Gary M. King

Faculty Publications

© 2017 Myers and King. The presence of hydrated salts on Mars indicates that some regions of its surface might be habitable if suitable metabolizable substrates are available. However, several lines of evidence have shown that Mars' regolith contains only trace levels of the organic matter needed to support heterotrophic microbes. Due to the scarcity of organic carbon, carbon monoxide (CO) at a concentration of about 700 parts per million (about 0.4 Pa) might be the single most abundant readily available substrate that could support near-surface bacterial activity. Although a variety of electron acceptors can be coupled to CO oxidation, …

Concise Review: Translating Regenerative Biology Into Clinically Relevant Therapies: Are We On The Right Path?, Jennifer Simkin, Ashley W. Seifert

Biology Faculty Publications

Despite approaches in regenerative medicine using stem cells, bio-engineered scaffolds, and targeted drug delivery to enhance human tissue repair, clinicians remain unable to regenerate large-scale, multi-tissue defects in situ. The study of regenerative biology using mammalian models of complex tissue regeneration offers an opportunity to discover key factors that stimulate a regenerative rather than fibrotic response to injury. For example, although primates and rodents can regenerate their distal digit tips, they heal more proximal amputations with scar tissue. Rabbits and African spiny mice re-grow tissue to fill large musculoskeletal defects through their ear pinna, while other mammals fail to regenerate …

Father Involvement Among Asian-Indian Immigrants In The United States: Actor-Partner Interdependence Model, Dimple P. Vadgama

Dissertations - ALL

Parenting is codependent and nested within a familial and cultural structure. While parenting research consistently demonstrates more maternal involvement with children, often fathers’ involvement gets little or no attention. One of the major limitations of fathering research is single source data, often comprised of only mothers’ reports of fathers’ involvement. The purpose of this study was to address this gap by examining the nested nature and interdependence of immigrant parents’ marital adjustment, parenting self-efficacy, and beliefs about parental role and, fathers’ involvement. Actor-partner interdependence model (APIM) was applied to examine the actor (intrapersonal or spillover) and partner (interpersonal or crossover) …

Development And Evaluation Of A Connective Tissue Phantom Model For Subsurface Visualization Of Cancers Requiring Wide Local Excision, Kimberly S. Samkoe, Brent D. Bates, Niki N. Tselepidakis, Alisha V. Dsouza, Jason R. Gunn, Dipak B. Ramkumar, Keith D. Paulsen, Brian W. Pogue, Eric R. Henderson

Dartmouth Scholarship

Wide local excision (WLE) of tumors with negative margins remains a challenge because surgeons cannot directly visualize the mass. Fluorescence-guided surgery (FGS) may improve surgical accuracy; however, conventional methods with direct surface tumor visualization are not immediately applicable, and properties of tissues surrounding the cancer must be considered. We developed a phantom model for sarcoma resection with the near-infrared fluorophore IRDye 800CW and used it to iteratively define the properties of connective tissues that typically surround sarcoma tumors. We then tested the ability of a blinded surgeon to resect fluorescent tumor-simulating inclusions with ∼1-cm margins using predetermined target fluorescence intensities …

Concordance And Discordance: Cognitive And Neuropsychological Performance Of Twins With Autism Spectrum Disorder, Elizabeth P. Mckernan

Theses - ALL

This study sought to examine the cognitive and neuropsychological performance of monozygotic twin pairs who vary in concordance for ASD. The study used secondary data collected from a larger pilot study, resulting in a final sample of 28 children, all of whom were monozygotic twins (IQ range: 36 – 109). First, the study sought to determine whether pairwise concordance for ASD among monozygotic twins has changed with the use of new diagnostic criteria. McNemar tests found that pairwise concordance rates were not significantly different across DSM-IV-TR and DSM-5 diagnostic criteria, but were significantly greater with the use of either parent …

Oxygen Transport Membrane Reactors For Oxy-Fuel Combustion And Carbon Capture Purposes, Ryan Falkenstein-Smith

Dissertations - ALL

This thesis investigates oxygen transport membrane reactors (OTMs) for the application of oxy-fuel combustion. This is done by evaluating the material properties and oxygen permeability of different OTM compositions subjected to a variety of operating conditions. The scope of this work consists of three components: (1) evaluate the oxygen permeation capabilities of perovskite-type materials for the application of oxy-fuel combustion; (2) determine the effects of dual-phase membrane compositions on the oxygen permeation performance and membrane characteristics; and (3) develop a new method for estimating the oxygen permeation performance of OTMs utilized for the application of oxy-fuel combustion.

SrSc0.1Co0.9O3-δ (SSC) is …

Wifely Figures: Gender, Marriage, And Biblical Typology In Early Modern England, Melissa Welshans

Dissertations - ALL

This dissertation illuminates how the hermeneutic of biblical typology influenced the conception of the female life cycle in early modern English literature, especially the social roles of maid, wife, and widow. Reading texts from a variety of genres and by both male and female authors, this dissertation argues that a typological understanding of marriage gave additional, spiritual import to those social roles, thus further upholding ideologies that defined women by their proximity to marriage. However, this dissertation also demonstrates how a typological understanding of marriage and the female life cycle could also be used to critique gender norms. After providing …

Vortex Detection And Tracking In Massively Separated And Turbulent Flows, Yangzi Huang

Dissertations - ALL

The vortex produced at the leading edge of the wing, known as the leading edge vortex (LEV), plays an important role in enhancing or destroying aerodynamic force, especially lift, upon its formation or shedding during the flapping flight of birds and insects. In this thesis, we integrate multiple new and traditional vortex identification approaches to visualize and track the LEV dynamics during its shedding process. The study is carried out using a 2D simulation of a flat plate undergoing a 45 degree pitch-up maneuver. The Eulerian 𝛤1 function and 𝑄 criterion are used along with the Lagrangian coherent structures (LCS) …

The Post-Neoliberal Citizen: Immigrant Identity As The New Service Learning Center, Michael Lasley

Dissertations - ALL

In my dissertation, The Post-Neoliberal Citizen: Immigrant Identity as the New Service Learning Center, I analyze the language of service-learning initiatives, particularly the language these initiatives use regarding global citizenship, through the lens of the rhetorics of neoliberalism. Much of service-learning scholarship has, I argue, taken for granted the idea of citizenship; that is, the political subjectivity of participants in these programs has been articulated from the perspective of American citizenship. I begin the dissertation by tracing the early conversations about citizenship within service-learning scholarship, and I then note how these conversations shift when second-wave service-learning scholars reframed service-learning to …

Design Of Tribologically Enhanced Polymeric Materials For Biomedical Applications, Allen Osaheni

Dissertations - ALL

Anytime two surfaces are in normal contact, accompanied by tangential motion, there is potential for deterioration of one or both surfaces. Gradual wear, or the removal of surface material, is typically an undesirable event. Therefore, the need for lubrication arises to minimize the amount of shear stress that develops between opposing surfaces. This reduction in shear stress is characterized by the coefficient of friction (COF). Friction is one of the primary subjects of interest in tribology, the science of the friction and wear of articulating surfaces.

A number of fascinating tribological systems can be found in nature. One example which …

Readers’ Perceptions Of Newsworthiness And Bias As Factors In Participation With Digital News Content, Gregory James Munno

Dissertations - ALL

Audience participation with digital news content has become a central feature of news consumption. These participatory news behaviors – commenting on, sharing, and “liking” news stories – have implications for both newsreaders and producers of news. This dissertation tests a structural model of commenting behavior using survey data (N = 335). The model builds on suggestions of a connection between hostile-media effects and commenting. This study adds newsworthiness to the structural equation, hypothesizing that newsworthiness increases readers’ perceptions that an article will influence other readers. These relationships should increase hostile-media effects, and, therefore, a reader’s likelihood of commenting. The model …

The Effects Of Gross-Motor Fluency Training On Physical Activity Levels In Young Children, Allison Jayne Womack

Dissertations - ALL

Engaging in regular physical activity is one of the most important factors related to good health. Encouraging and promoting physical activity in young children aids in preventing overweight and obesity, promoting health, preventing disease, and supporting bone and overall growth development. However, physical activity levels in young children are low, and efficient and effective interventions to increase physical activity in young children are unclear. One creative method to increase physical activity in young children is gross-motor fluency training. A multielement single-case experimental design was employed to assess the effects of fluency training in six gross-motor skills on children’s levels of …

Post Processing Of Confocal Microscopy Images To Quantify Collagen Remodeling By Fibroblasts, Zhang Liu

Mechanical Engineering and Materials Science Independent Study

The ways that fibroblast cells remodel their local microenvironment is critical to a number of pathologies. To quantify this in a 3D environment, I an existing MATLAB script was modified to process the qualitative, visual information from confocal microscopy images and generate quantitative graphs representing the density of collagen around a fibroblast as a function of distance from the cell. While the earlier script used each 2D image from a confocal image stack and dilated the shape of the recognized cell to extract to find the coordinates of the surrounding collagen. The work in this study builds on the analysis …

Developing A “Sense Of Community”: Exploring A Cognitive Component Of Civic Engagement, Tabitha Underwood

eJournal of Public Affairs

Civic engagement has become an important component of higher education practice that assists colleges and universities in fulfilling their civic mission and public purpose. This reflective essay outlines an oftentimes forgotten aspect of that work—how we integrate students into the life of a community and help them develop a sense of interdependence with it. The concept of “sense of community” allows this opportunity and should be incorporated in higher education professions as both a guiding principle and an outcome of engaged scholarship. The author outlines the idea of incorporating the sense of community concept into higher education civic engagement, including …

Book Review: Scientists, Experts, And Civic Engagement: Walking A Fine Line, Alexander Wait

eJournal of Public Affairs

No abstract provided.

Growing Pains: The Transformative Journey From A Nascent To A Formal Not-For-Profit Venture, Avery C. Edenfield, Fredrik O. Andersson

English Faculty Publications

This article examines how a social venture transitions from nascent to formal status and argues that the transformation of the organization set in motion by establishing formal boundaries is a deeply profound one. Drawing from the nonprofit and social entrepreneurship literature on what prompts and energizes individuals to initiate new not-for-profit ventures, and linking it to a notion of revolutionary crisis as organizations emerge and develop, we seek to illuminate and explore the tension, and its consequences, between nonprofit entrepreneurs and the organization they create as the new venture transitions from nascent to formal. We do this by presenting the …

Structural Evidence Of A Major Conformational Change Triggered By Substrate Binding In Dape Enzymes: Impact On The Catalytic Mechanism, Boguslaw Nocek, Cory Reid, Anna Starus, Tahirah Heath, David Bienvenues, Jerzy Osipiuk, Robert Jedrzeczak, Andrzej Joachimiak, Daniel P. Becker Ph.D., Richard C. Holz

Chemistry: Faculty Publications and Other Works

The X-ray crystal structure of the dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase from Haemophilus influenzae (HiDapE) bound by the products of hydrolysis, succinic acid and l,l-DAP, was determined at 1.95 Å. Surprisingly, the structure bound to the products revealed that HiDapE undergoes a significant conformational change in which the catalytic domain rotates ∼50° and shifts ∼10.1 Å (as measured at the position of the Zn atoms) relative to the dimerization domain. This heretofore unobserved closed conformation revealed significant movements within the catalytic domain compared to that of wild-type HiDapE, which results in effectively closing off access to …

Achilles And The Batman On The Plane Of Immanence: Deconstructing Heroic Models, Herman Frederick Filice

Dissertations - ALL

Achilles and The Batman on the Plane of Immanence: Deconstructing Heroic Models attempts to reposition the way the reader views literary heroism. By defining heroism as a reaction to forces within and external to his society, heroism becomes a function of Michel Foucault’s concept of Otherness. Using Deleuze and Guattari’s notions of the Nomad, striated and unstriated space and schizoanalysis, as well as Foucault’s basic concepts of power and its two-way, invisible flow, one will find that heroism develops and functions in strikingly similar manners no matter what time or culture germinates it. The universal problems the hero creates for …