Digital Commons Network^™

Examining The Role Of Host Use On Divergence In The Redheaded Pine Sawfly, Neodiprion Lecontei, Across Multiple Spatial Scales, Robin Kimberly Bagley

Theses and Dissertations--Biology

Phytophagous insects make up over one quarter of described species on Earth, and this incredible diversity seems directly linked to feeding on plants. Comparative studies of sister groups have shown shifts to herbivory are consistently associated with increased species diversity in insects, but the reasons for this diversification remain unclear. While other explanations, such as decreased extinction rates or influences on population structure, exist, one prominent hypothesis suggests shifts and subsequent adaptation to novel host plants can lead to the evolution of reproductive barriers.

Given their extreme specialization on host plants in the genus Pinus and intimate, life-long association with …

‘Something A Little Bit Tasty’: Women And The Rise Of Nutrition Science In Interwar British Africa, Lacey Sparks

Theses and Dissertations--History

Widespread malnutrition after the Great Depression called into question the role of the British state in preserving the welfare of both its citizens and its subjects. International organizations such as the League of Nations, empire-wide projects such as nutrition surveys conducted by the Committee for Nutrition in the Colonial Empire (CNCE), sub-imperial networks of medical and teaching professionals, and individuals on-the-spot in different colonies wove a dense web of ideas on nutrition. African women quickly became the focus of efforts to end malnutrition due to Malthusian concerns of underpopulation in Africa and African women’s role as both farmers and mothers. …

Feeding The Soul: Voices Of Kentucky Women Combating Child Hunger, Mya Oneisha Price

Theses and Dissertations--Community & Leadership Development

This study addresses the overarching topic of food insecurity by giving voice to individuals who are dedicating their careers toward combating child food insecurity throughout their communities. Voices are uplifted through the representation of narratives by volunteer coordinators overseeing child feeding programs, which have been established throughout Kentucky as an effort to help alleviate child hunger. This study is guided by London’s theory on career motivation, with the outcomes of this study serving as a pilot for future research centered around individuals working to combat child food insecurity. The narratives collected from this study will be used as a resource …

Riding Through Life: A Lifespan Study Of The Attitudes, Behaviors, And Areas Of Educational Opportunity For Female Equestrians Toward Bra Use And Health Outcomes When Engaged In Equestrian Sports, Karin Pekarchik

Theses and Dissertations--Community & Leadership Development

Equestrian sport is a popular exercise choice for females of all ages, although the links between this exercise choice, health outcomes, and risk to health have not been thoroughly examined. These issues are of particular interest because the female equestrian has the opportunity to ride and/or compete for decades, from early to very late in life, covering almost the full sequence of the female lifespan. Equestrian sports provide physical activity through riding and caring for the horse, and promote positive mental outcomes through tangible practices, such as engaging in physical activity and hard work, goal-setting, acquisition of skills, and participation …

A Portable Sensor For Measuring Gas Emissions From Dairy Compost Bedded Pack Barns, Katharine Wolf

Theses and Dissertations--Biosystems and Agricultural Engineering

The objective of this study was to develop a gas measurement chamber for the comparison of emissions from different dairy manure storage facilities. Compost bedded pack (CBP) barns are a loose housing system in which cows rest on an intensely managed compost pack. Sawdust is the primary material added to the system, along with manure and urine inputs from the cows, and the pack is stirred one to two times daily. Maintaining a high level of aerobic microbial activity in the pack is critical for cow health. Previous dairy emissions work has not included compost bedded pack barns; it was …

The Development Of Bsn272 From Prevention Of Diet-Induced Hyperlipidemia In Mice To A Potential Therapy For Prader-Willi Syndrome In Humans, Jarrod B. Williams

Theses and Dissertations--Pharmacy

While type 2 diabetes mellitus (T2DM) is distinguished as a disorder of blood glucose homeostasis, the vast majority of patients afflicted with this disease in the US present with the entire complement of the metabolic syndrome: abdominal obesity, hypertriglyceridemia, low HDL levels, elevated blood pressure, and elevated fasting plasma glucose. Current guidelines aim to treat each of these disorders, but do so individually even though they are manifestations of common pathologies. In other words, a patient will be prescribed different medications for diabetes, blood pressure, CVD prevention, etc., while the only single treatment aimed at the myriad of disorders together …

Effects Of In Utero Nicotine Exposure On Immune Cell Disposition After P. Aeruginosa Lung Infection, Nayon Kang

Theses and Dissertations--Pharmacy

Current smoking cessation guidelines recommend nicotine replacement therapy (NRT) to assist pregnant smokers to quit, but this is without strong evidence for effectiveness and safety. Nicotine, the main addictive component of tobacco, is known to exert physiological effects by binding to its receptor, the nicotinic acetylcholine receptor (nAChR). Recent studies have identified the presence of nAChRs in non-neuronal cells, and in macrophages, functional alteration upon stimulation with nicotine has been documented.

To understand the impact of in utero nicotine exposure on various immune cell disposition and function, we designed preliminary studies using an in vivo model of P. aeruginosa infection. …

An Exploratory Statistical Method For Finding Interactions In A Large Dataset With An Application Toward Periodontal Diseases, Joshua Lambert

Theses and Dissertations--Epidemiology and Biostatistics

It is estimated that Periodontal Diseases effects up to 90% of the adult population. Given the complexity of the host environment, many factors contribute to expression of the disease. Age, Gender, Socioeconomic Status, Smoking Status, and Race/Ethnicity are all known risk factors, as well as a handful of known comorbidities. Certain vitamins and minerals have been shown to be protective for the disease, while some toxins and chemicals have been associated with an increased prevalence. The role of toxins, chemicals, vitamins, and minerals in relation to disease is believed to be complex and potentially modified by known risk factors. A …

The Effects Of Idea Part C Early Intervention Services On The Well-Being Of Children And Families In Child Welfare, Ching-Shu J. Fang

Theses and Dissertations--Social Work

There is ample evidence indicating that maltreatment has deleterious effects on the development of infants and toddlers. The Child Abuse Prevention and Treatment Act of 2003 requires referrals from child welfare (CW) to IDEA Part C Early Intervention services to provide developmental assessments and services for children younger than three with substantiated cases of child abuse or neglect. Thus, this study aims to examine the effects of Part C services on the well-being of young children and their families in CW.

This study used a secondary dataset, the National Survey of Children and Adolescent Well-Being II, to examine the research …

An Exploratory Study Of Incapacitated Persons With Criminal Behaviors Served By Kentucky’S Public Guardianship Program, Karen Martin

Theses and Dissertations--Social Work

State run public guardianship programs are legally mandated to provide custodial care for persons deemed incapacitated by the courts. Historically, the majority of state wards were elderly women residing in skilled nursing facilities. Today, those demographics are rapidly changing. This new incapacitated cohort has become less institutionalized, with a rising number of persons who have entered the program with criminal records and who continue to commit crimes. This exploratory study focuses on incapacitated persons (IP) with criminal behaviors in order to seek what intervention(s) might reduce their criminal activity. Relying on routine activity theory, differing levels of supervision were compared …

The Impact Of Attitudes And Beliefs About Fat On Social Work Education In Appalachia: An Exploratory Study, Genesia Kilgore-Bowling

Theses and Dissertations--Social Work

Anti-fat bias and the resulting discriminatory behavior is widely documented and impacts almost every aspect of an obese person’s life, including healthcare/insurance, education, employment, interpersonal relationships, and protection under the law. This has serious psychological, social, physical health, and economic consequences for the obese person. The non-social work related literature provides an abundance of evidence that when obese people seek assistance from a helping professional, they are met with the same anti-fat bias and discrimination present in other areas of their lives.

Recognizing that anti-fat bias can lead to negative practice behaviors with obese patients and clients, many professional education …

A Grounded Theory Study Of Maternal Engagement Of Low Income, Young Rural Mothers In Home-Based, Early Intervention Services, Elaine K. Fehringer

Theses and Dissertations--Rehabilitation Sciences

Home-based, early intervention programs as a preferred delivery model are widely endorsed, heavily funded and highly utilized as prevention and remediation initiatives for families with young children (Eckenrode, et al., 2010; Karoly, Killion, & Cannon, 2005). Of concern, is that while an estimated 40 billion dollars are spent annually between federally funded programs and private foundations, a significant number of families disengage from services before the end of a child’s eligibility period (Stevens, Ammerman, Putnam, Gannon, & van Ginkel, 2005). Several meta-analyses indicate only modest effectiveness of home-based services (Tandon, et al., 2008). It is estimated that well over 500,000 …

Telomerase Reverse Transcriptase In Atherosclerosis, Hua Qing

Theses and Dissertations--Pharmacology and Nutritional Sciences

Telomerase reverse transcriptase (TERT) is the catalytic subunit of telomerase and the limiting factor for the enzyme activity. The expression of TERT and telomerase activity is increased in atherosclerotic plaques. However, the role of TERT dysregulation during atherosclerosis formation remains unknown.

The work herein first identified a multi-tiered regulation of TERT expression in smooth muscle cells (SMC) through histone deacetylase (HDAC) inhibition. HDAC inhibition induces TERT transcription and promoter activation. At the protein level in contrast, HDAC inhibition decreases TERT protein abundance through enhanced degradation, which decreases telomerase activity and induces senescence. Furthermore, during vascular remodeling in vivo, TERT protein …

The Role Of Progesterone Receptor Membrane Component 1 In Receptor Trafficking And Disease, Kaia K. Hampton

Theses and Dissertations--Pharmacology and Nutritional Sciences

The progesterone receptor membrane component 1 (PGRMC1) is a multifunctional protein with a heme-binding domain that promotes cellular signaling via receptor trafficking, and is essential for some elements of tumor growth and metastasis. PGRMC1 is upregulated in breast, colon, lung and thyroid tumors. We expanded the analysis of PGRMC1 in the clinical setting, and report the first analysis of PGRMC1 in human oral cavity and ovarian tumors and found PGRMC1 to correlate with lung and ovarian cancer patient survival. Furthermore, we discovered a specific role for PGRMC1 in cancer stem cell viability. PGRMC1 directly associates with the epidermal growth factor …

Insulin Actions On Hippocampal Neurons, Shaniya Maimaiti

Theses and Dissertations--Pharmacology and Nutritional Sciences

Aging is the main risk factor for cognitive decline. The hippocampus, a brain region critical for learning and memory formation, is especially vulnerable to normal and pathological age-related cognitive decline. Dysregulation of both insulin and intracellular Ca2+ signaling appear to coexist and their compromised actions may synergistically contribute to neuronal dysfunction with aging. This dissertation focused on the interaction between insulin, Ca2+ dysregulation, and cognition in hippocampal neurons by examining the contributions of insulin to Ca2+ signaling events that influence memory formation. I tested the hypothesis that insulin would increase cognition in aged animals by altering Ca2+-dependent physiological mechanisms involved …

The Relationship Between Modifiable Lifestyle Factors And Muscle Lipid Depots In Older Adults, Maja Redzic

Theses and Dissertations--Pharmacology and Nutritional Sciences

Muscle health declines with age, influencing both muscle strength and performance. Modifiable lifestyle factors may mitigate muscle dysfunction by altering muscle lipid depots. Vitamin D status, dietary intake, and physical activity each play a role in muscle health during aging. Low vitamin D status (measured as 25(OH)D) is prevalent among older adults and has been associated with functional limitations and impaired physical performance. Previously, 25(OH)D concentrations were inversely associated with extramyocellular lipid (EMCL) and our lab observed a significant positive relationship between 25(OH)D concentrations and IMCL. Studies have shown mixed results regarding the effects dietary fat intake interventions have on …

New Insights Into Post-Sepsis Muscle Weakness Elucidated Using A Novel Animal Model, Allison M. Steele

Theses and Dissertations--Physiology

Sepsis is a severe life-threatening critical illness that damages multiple physiological systems. After hospital discharge, more than 70% of severe sepsis survivors report profound weakness which significantly impacts quality of life. Such weakness gives rise to new limitations of daily living, which ultimately leads to loss of independence in many patients. Despite wide recognition of this serious issue by clinicians and researchers alike, the mechanisms contributing to chronic skeletal muscle dysfunction after sepsis are not well understood. Lack of progress in this field is largely due to the absence of an appropriate animal model; current models are either too mild …

Role Of Skeletal Paracrine Signals In The Proliferation And Chondrogenic Differentiation Of Interzone Cells, Parvathy Thampi

Theses and Dissertations--Veterinary Science

Articular cartilage in mammals has a limited intrinsic capacity to repair structural injuries and defects, a fact that contributes to the chronic and progressive nature of osteoarthritis. Current treatment modalities do not enable articular cartilage to achieve a complete and permanent restoration of normal structure and function with large or partial thickness lesions. In contrast to mammals, Mexican axolotl salamanders (Ambystoma mexicanum) have demonstrated the remarkable ability to spontaneously and completely repair even large joint cartilage lesions, an intrinsic healing process that involves interzone cells in the intraarticular space. Further, when interzone tissue is transplanted into critical sized …

Survival And Cause-Specific Mortality Of White-Tailed Deer (Odocoileus Virginianus) Neonates In A Southeastern Kentucky Population, Joseph R. Mcdermott

Theses and Dissertations--Forestry and Natural Resources

Maintaining desired numbers of wildlife species requires an understanding of species-specific population dynamics. For ungulate species such as the white-tailed deer (Odocoileus virginianus), understanding the neonatal survival rate of a population and factors that influence that survival rate, may be two of the most important factors to successful deer management. We examined neonatal survival in an eastern Kentucky population of deer living in relatively low densities (/ km²), with adequate habitat and supposedly poor population growth. Neonates (102) were captured in the summer birth periods of 2014 - 2016 and radio-monitored until the beginning of the …

Analysis Of Humoral Immune Responses In Horses With Equine Protozoal Myeloencephalitis, Catherine-Jane Angwin

Theses and Dissertations--Veterinary Science

Equine protozoal myeloencephalitis (EPM), caused by the protozoan parasite Sarcocystis neurona, is one of the most important neurological diseases of horses in the Americas. While seroprevalence of S. neurona in horses is high, clinical manifestation of EPM occurs in less than 1% of infected horses. Factors governing the occurrence and severity of EPM are largely unknown, although horse immunity might play an important role in clinical outcome. We hypothesize that EPM occurs due to an aberrant immune response, which will be discernable in the equine IgG subisotypes a, b, and (T) that recognize S. neurona in infected diseased horses versus …

Inflammation And Insulin Dysregulation In The Horse, Sarah E. Elzinga

Theses and Dissertations--Veterinary Science

Insulin dysregulation (ID) in the horse is receiving increasing attention as a serious health concern, in particular due to its association with the potentially career or life ending condition, laminitis. The role of inflammation and the immune system in ID as well as its associated health concerns has also been raised. However, the involvement of inflammation in and mechanisms behind ID in the horse remain unclear. Therefore, our overall hypothesis was that due in part to changes in their gut microbiota and plasma lipidome, horses with ID have changes in circulating proinflammatory markers, in particular in response to glycemic challenge, …

Lymphocyte-Mediated Inflamm-Aging In The Horse, Melissa Hope Siard

Theses and Dissertations--Veterinary Science

Senior horses (≥20 years) exhibit inflamm-aging, or chronic, low-grade inflammation that occurs systemically with aging, similarly to humans. Inflamm-aging has previously been characterized in the horse in circulation as well as specifically being mediated by lymphocytes and monocytes. In humans, inflamm-aging has been associated with increased morbidity and mortality. However, in the horse, relatively little about inflamm-aging is known regarding clinical effects or factors influencing severity. The contribution of lymphocytes to inflamm-aging of senior horses was examined, specifically through determining the relationships of inflamm-aging with various other health parameters, effects of seasonality, and the extent to which inflamm-aging can be …

Physiological Changes Associated With Pregnant Or Nonpregnant Mares Grazing Pastures Of Orchardgrass-Bluegrass, Kentucky 31 Tall Fescue Infected With Epichloë Coenophiala, Or Kyfa9821 Tall Fescue Infected With The Novel Endophyte Ar584, Victoria A. Taylor

Theses and Dissertations--Veterinary Science

Kentucky 31 tall fescue (KY31) infected with the common toxic endophyte strains of Epichloё coenophiala produces toxic alkaloids that improve plant vigor, but cause numerous adverse effects in grazing animals. Researchers developed a variety of KY31 containing an alternative strain of E. coenophiala, termed novel endophyte (NE). Adverse health effects in mares have not been evaluated.

Experiments in this thesis tested the hypothesis that the NE pasture does not cause adverse effects typically associated with KY31. Specific aims were to: 1) compare forage ergovaline concentrations between KY31 vs NE pastures; 2) evaluate palmar artery diameters in mares grazing KY31, …

Establishment Of Serological Reference Ranges For Elk In Kentucky And Effects Of Quarantine And Translocation On Elk, Aaron M. Hildreth

Theses and Dissertations--Forestry and Natural Resources

Establishing blood serum profiles is important for understanding animal disease ecology and nutrition, the effects of capture and immobilization, and general physiological variation among individuals and populations. Elk (Cervus elaphus nelsoni) have been successfully translocated to several states for reintroduction or population augmentation, including most recently in Kentucky, where over the course of three years (2011-13) wild elk were captured, temporarily quarantined, and subsequently moved to Missouri and Virginia to establish populations in those states. I used this opportunity to collect a variety of biological samples, including blood from which I present and compare serological profiles for a …

Survival And Cause-Specific Mortality Of A Southeastern Kentucky Deer Population, Caleb Anderson Haymes

Theses and Dissertations--Forestry and Natural Resources

White-tailed deer are one of the most sought after game species in Kentucky. While much of the Commonwealth boasts high deer populations, those in southeast Kentucky are viewed as relatively low compared to other regions, even after a decade of restrictive doe harvest and multiple years of population supplementation via translocation. We studied survival and cause specific mortality of a local population of deer near the Redbird District of the Daniel Boone National Forest in Clay and Leslie County, Kentucky from January 2014 - January 2017. We estimated female annual survival at 0.89 (CI: 0.88-0.87), with an overall 3-year survival …

Comparing Mindfulness-Enriched Weight Management To Current Standard Practices, Teresa M. Lee

Theses and Dissertations--Nutrition and Food Systems

Mindfulness-based interventions have been shown to be effective for numerous diet-related conditions. Mindfulness skills have been theorized to be helpful in improving eating behaviors, and thereby weight management. The purpose of this study was to compare the effectiveness of a mindfulness-enriched weight management program to a standard weight loss program over the course of a 12-week intervention, and weight maintenance over six months. This was a two-group randomized experimental design. One group received a standard weight loss program, while the other group received the same program with an additional mindfulness component. Follow up assessments were conducted twice at three-month intervals. …

Social Marketing Campaign At Farmers' Markets To Encourage Fruit And Vegetable Purchases In Rural Counties With A High Obesity Prevalence, Emily M. Dewitt

Theses and Dissertations--Nutrition and Food Systems

Farmers’ markets are one food environment venue with the potential to increase fruit and vegetable consumption among rural residents in the United States. This study used the Plate It Up Kentucky Proud social marketing campaign in six rural communities over two years to determine the association between exposure to the campaign and fruit and vegetable purchasing decisions. The availability of recipe cards at the farmers’ market was associated with influencing the purchase of fruits and vegetables (p<0.001); the recipe sample was associated with buying the ingredients for the recipe (p<0.001); taking the PIUKP food sample was associated with a willingness to prepare the food item at home (p<0.001). Utilizing social marketing strategies at farmers’ markets may be an effective way to improve fruit and vegetable intakes in rural communities.

Changes Of Percent Body Fat, Waist Circumference And Fruit And Vegetable Intake Among Division I Collegiate Female Softball Players After Nutrition Curriculum Paired With Technology, Rachel Thomas

Theses and Dissertations--Nutrition and Food Systems

Previous research has found that the quality and intake of collegiate athletes’ diets does not meet the recommended standards. Little research has been completed in regards to the diets of collegiate female softball players. The purpose of this research study was to determine if an 11-week nutrition curriculum paired with a technology could increase fruit and vegetable consumption while decreasing percent body fat and waist circumference. The sample included 14 female softball players. Paired t-test were preformed to compare fruit and vegetable intake, body fat percentage, and waist circumference pre- and post-intervention. Linear regression models were used to determine correlations …

Couples And Weight Loss Surgery: Experiencing Success, Amanda L. Westmoreland

Theses and Dissertations--Family Sciences

Bariatric surgery, also known as weight loss surgery (WLS) is an intervention for individuals who are suffering from obesity and weight-related health complications which often accompany being 100 pounds or more overweight (Smith et al., 2011). Even though bariatric surgery has been shown to be a life-saving and life-enhancing operation, efforts to seek out surgery options, qualify and prepare for the procedure, recover from surgery, and then adapt new routines to support surgery are challenging not only for the patient, but also for the people with whom the patient spends the most time—their romantic partners (Applegate & Friedman, 2008; Bylund, …

Finding The Link Between Social Connectivity And Dietary Intake Among Rural Adolescents In North Carolina And Kentucky, Jordan Elizabeth Mcdonald

Theses and Dissertations--Nutrition and Food Systems

Social networks play a significant role in adolescent decision making, specifically when it comes to dietary outcomes. This study, granted by the United States Department of Agriculture (USDA), assessed the connectivity of these social networks and the impact they have on fruit and vegetable, added sugar and sugar sweetened beverage consumption. Additionally, the relationship between shopping companionship and dietary choices was studied. Positive and negative associations were found among adolescents who shop with parents or friends. It was also found that those adolescents with greater social network cohesion were found to have more negative dietary outcomes. Divulging further into the …