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Molecular Diagnosis Of Caseous Lymphadenitis In Sheep At Dakahlia Governorate, Egypt, A Selim, S Atwa, A El-Gedawy, A Younis

Mansoura Veterinary Medical Journal

The aim of the present study was to diagnose caseous lymphadenitis in sheep by PCR assay . A total of 1257 sheep from different flocks in Dakahlia governorate were clinically examined during the period of January 2014 to February 2015. sheep were classified according to the status of infection in the flocks to infected , incontact and free ones. 37 needle biopsy samples were randomly collected from 26 diseased, 7 incontact and 4 free examined sheep for PCR assay. rpoB gene was detected in 37 samples (100%) while PLD gene was in 18 samples (48.64%), from which 16 from diseased …

Studies On H9 Avian Influenza In Dakahlia Governorate, Asmaa Alzayat, Abdelfattah Eladl, Hanan Fahmy, Hany Ellakany

Mansoura Veterinary Medical Journal

Prevalence of H9 Avian Influenza Virus (AIV-H9) in Dakahlia governorate during 2012 - 2015 was undertaken to update the epidemiology of avian influenza virus infections among poultry flocks in Egypt. A total of 50 chicken flocks were examined (28 broiler flock and 22 layer flock). Results revealed that 18 samples were HA positive while 7 out of 18 samples were positive by chromatographic strip test for common antigen of AIV. Two chicken flocks were found positive AIVH9 by RT-PCR and qrt-PCR. The virus was successfully isolated and propagated in SPF eggs. The result was screened by HA and confirmed by …

Studies On Currently Circulating Paramyxoviruses In Pigeons, Reham Elbhnsawy, K Abu El Azm, Sanaa Awad, A Eladl

Mansoura Veterinary Medical Journal

Twenty two pigeon paramyxovirus (PPMV) isolates were recovered from 50 tested diseased pigeons. Tested pigeons suffered from nervous manifestation, listlessness, inappetance, inability to fly and greenish diarrhea. Diseased pigeons were collected from Dakahlia governorate during 2014 and 2015. Virus isolation was done in embryonated chicken eggs via allantoic sac inoculation. Isolates were identified as PMV by HA and RT-PCR tests. Pathogenicity for five isolates was done via intracerebral inoculation of one day old 10 SPF-chicks for each isolate. Intracerebral pathogenicity index varied from 0.0 to 1.44. Although RT-PCR testing of liver, spleen, lung and brain collected from culled chicks on …

Genetic Selection Of Some Immunological Traits In Local Egyptian Chicken Strain, Kh El-Bayomi, F Abd El-Ghany, R Darwish, Nagwan Ahmed

Mansoura Veterinary Medical Journal

This work was carried out to investigate the effectiveness of selection for high immune response against Newcastle Disease Virus (NDV) vaccine in local Egyptian chicken strain (Dokki4) for two generations. Two lines (selected and control) of Dokki-4 strain were used; in the selected line, chicks were selected according to its immune response against NDV vaccine. In the control line, chicks were randomly chosen. The population families were vaccinated against the common prevalent diseases according to the vaccination programs in the Sakha Animal Production Research Station, Kafr El-Sheikh. Results of selection for high immune response against NDV vaccine showed that; selection …

Molecular Detection And Serotyping Of Foot And Mouth Disease Virus In Tongue Epithelium Samples, A. El-Kenawy, E. Younis, Hiam Fakhry, R. Karam

Mansoura Veterinary Medical Journal

The aim of the present study is molecular identification and typing of FMDV circulating in Dakahlia governorate in Egypt, 2014. The study was accomplished on pooled nine tongue epithelium samples collected from diseased cattle and buffaloes expected to be infected with FMDV. The diseased animals showed fever , excessive salivation and ulceration on tongue and gum. Conventional reverse transcription polymerase chain reaction (RT-PCR) was used for identification of FMDV using universal primers. Then serotyping of the identified virus was done using RT-PCR for serotype O and A and Real time RT-PCR for serotype SAT2 . FMDV was identified in all …

Lyme Disease Ecology: Effects Of Habitat And Hosts On The Density And Distribution Of Borrelia Burgdorferi-Infected Ixodes Scapularis, Megan Ainsley Linske

Doctoral Dissertations

The research discussed in this dissertation examined host and habitat composition in Connecticut and their effect on the ecology of Lyme disease. Previous studies on management strategies sought a one-size-fits-all solution. However, Connecticut may require its own implementation of management strategies supported by research executed in its respective environment. Therefore, a novel approach that addresses the key components of a particular habitat, in this case Connecticut’s northern hardwood forests with an invasive plant understory, may yield better results than a “silver bullet” solution produced by a model or conducted in a different geographic location.

My research is conducted in Connecticut …

Neural Differentiation Of Adipose Tissue Derived Mesenchymal Stem Cells, A El-Gamal, A Abd-Elmaksaud, A Farag, H Marei

Mansoura Veterinary Medical Journal

The limited neurodegenerative power, makes from neural disorders the most challenging and difficult therapy among different diseases. till now, the traditional surgical and medical interventions only slow down the progression of these neurodegenerative diseases but the number of neurons are still diminish in many patients. Now, the mesenchymal stem cell (MSC) therapy has attracted the great attention of most scientists and clinicians around the world to be used as a therapeutic potential source for different neurodegenerative disorders. Stem cells have been found all over the body and can be obtained from different tissues as; adipose tissue, bone marrow and blood. …

Molecular Identification Of E. Coli And Salmonella Species Isolated From Fresh Water Fish By Polymerase Chain Reactions (Pcr), Viola Zaki, Adel El-Gohary, Mostafa Nabih

Mansoura Veterinary Medical Journal

Bacteriological examination of 400 fish samples taken from Oreochromis niloticus (O. niloticus), Clarias gariepinus, Cyprinus carpio.l (Common carp) and Mugil cephalus (100 from each) revealed isolation of (317) bacterial isolates, which were identified as 171 E.coli isolates and 146 Salmonella sp isolates with an incidence of (53.94% and 46.06 %) respectively. The incidence of E.coli and Salmonella spp. differ among different species of fish under investigation, the higher percentage of bacterial isolates observed in Clarias garipinas (53.31%), followed by (29.97 %), Cyprinus carpio.l (11.99 %), and the lower incidence was observed in Mugil cephalus (4.73%). Frequency distribution of isolated bacteria …

Detection For The Most Suitable Antibiotic And Disinfectant For Pseudomonas Aeruginosa Isolated From Pneumonic And Apparently Healthy Farm Animals, Neermin. Ibrahim, Yasser Elnaker, Salwa H, Ahmed. A.

Mansoura Veterinary Medical Journal

The present study was carried out on 550 samples (nasal, tracheal swabs and lung tissues) collected from pneumonic and apparently healthy cows and buffaloes. The bacteriological examination revealed that Pseudomonas aeruginosa was isolated from 172(41.1%) out of 419 cow’s samples and from 46(35.1%) out of 131 buffalo’s samples. from 172 cow’s isolates there were 144 isolates related to serotype (1) and 28 isolates were untypable while buffalo’s positive isolates revealed that 40 isolates were belonging to serotype (1) and 6 isolates were untypable. The effect of disinfectants as phenol, formalin and ethyl alcohol on viability of tested strains showed that …

Myxosporidiosis In Oreochromis Niloticus; Molecular Identification And Oxidative Stress Biomarkers, Nevien Abdelkhalek, M. El-Adl, M. Hamed, M. Al-Araby

Mansoura Veterinary Medical Journal

Myxosporidiosisis a protozoan parasitic fish disease that causes damages to fish organs and might eventually end with mortalities and deterioration of meat quality of the affected fish. The presence of parasitic infestation is usually accompanied with the production of reactive oxygen and nitrogen species that produces extra damage to host organs and inactivation of antioxidant defense system that increase the intensity of parasitic infestation. Recently, the response of the infested tissue to oxidative stress is varieddepending on the type of parasite; therefore the use of oxidative stress markers as indicator for myxobolus infestation is relevant. In the present study, Oreochromis …

Intestinal Exposure To Pcb 153 Induces Inflammation Via The Atm/Nemo Pathway, Matthew C. Phillips

Open Access Dissertations

Polychlorinated biphenyls (PCBs) are persistent organic pollutants that adversely affect human health. Although production of PCBs has stopped, they remain a pressing environmental problem due to their slow biodegradation and high lipophilicity. These properties enable PCBs to bio-accumulate in food chains leading to high levels of PCBs in the tissues of foodstuffs, especially fish. Despite dietary exposure being one of the main routes of exposure to PCBs, the gut has been widely ignored when studying the effects of PCBs. We set out to investigate the effects of PCB 153, the most environmentally prevalent PCB, on the gut, looking specifically for …

Serum Protein Analysis As An Immune Assessment Tool In South Florida Nurse Sharks, Ginglymostoma Cirratum, Leila Atallahbenson

Open Access Theses

Assessment of shark health can provide valuable insight into overall population health. In this study, immunological assessment tools are reviewed for their use with wild shark populations. Nurse sharks, Ginglymostoma cirratum, were non-lethally blood sampled from wild individuals in south Florida. Reference ranges are given for the first time for serum protein electrophoresis, which included five fractions that correspond closely with other elasmobranch species. Differences between serum and plasma may be negligible for protein electrophoresis, and therefore, plasma may be preferred in field settings. Reference ranges for other biometric and blood parameters were also established. The granulocyte to lymphocyte …

Evaluation Of Animal-Based Indicators To Be Used In A Welfare Assessment Protocol For Sheep, Susan E. Richmond, Françoise Wemelsfelder, Ina Beltran De Heredia, Roberto Ruiz, Elisabetta Canali, Cathy M. Dwyer

Farm Animal Welfare Collection

Sheep are managed under a variety of different environments (continually outdoors, partially outdoors with seasonal or diurnal variation, continuously indoors) and for different purposes, which makes assessing welfare challenging. This diversity means that resource-based indicators are not particularly useful and, thus, a welfare assessment scheme for sheep, focusing on animal-based indicators, was developed. We focus specifically on ewes, as the most numerous group of sheep present on farm, although many of the indicators may also have relevance to adult male sheep. Using the Welfare Quality® framework of four Principles and 12 Criteria, we considered the validity, reliability, and feasibility of …

Structural And Functional Insights Into The Role Of Bamd And Bame Within The Β-Barrel Assembly Machinery In Neisseria Gonorrhoeae, Aleksandra E. Sikora, Igor H. Wierzbicki, Ryszard A. Zielke, Rachael F. Ryner, Konstantin V. Korotkov, Susan K. Buchanan, Nicholas Noinaj

Molecular and Cellular Biochemistry Faculty Publications

The β-barrel assembly machinery (BAM) is a conserved multicomponent protein complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. Given its role in the production of OMPs for survival and pathogenesis, BAM represents an attractive target for the development of therapeutic interventions, including drugs and vaccines against multidrug-resistant bacteria such as Neisseria gonorrhoeae. The first structure of BamA, the central component of BAM, was from N. gonorrhoeae, the etiological agent of the sexually transmitted disease gonorrhea. To aid in pharmaceutical targeting of BAM, we expanded our studies to BamD and BamE within …

The Analytical Methods Used To Detect Fluoride In Dental Pharmaceuticals, Amber Aboona

Honors College Theses

My thesis, “The Analytical Methods Used to Detect Fluoride in Dental Pharmaceuticals” discusses the various analytical chemistry methods which are incorporated in the field of dentistry in order to detect fluoride in pharmaceuticals such as toothpaste and mouthwash. The oral cavity is exposed to various forms of bacteria, many of which enter through the consumption of foods and drinks that contain sugar and starch. The side effects of fluoride have been a controversial component of oral healthcare, however, many benefits have been discussed. Water fluoridation has been one of many significant methods of incorporating fluoride into the oral cavity, enhancing …

Introducing Volume 3, Editorial Board

Spora: A Journal of Biomathematics

This report provides a brief introduction to Spora and what you can expect from the articles included in this volume.

Effects Of Leukoreduction And Storage On Erythrocyte Phosphatidylserine Expression And Eicosanoid Concentrations In Units Of Canine Packed Red Blood Cells, Samantha Muro

Theses and Dissertations

Storage of canine packed red blood cells (pRBCs) can increase erythrocyte phosphatidylserine (PS) expression and eicosanoid concentrations. The objective of this study was to determine the effects of leukoreduction on erythrocyte PS expression and eicosanoid concentrations in stored units of canine pRBCs. Units of whole blood were leukoreduced (LR) or non-leukoreduced (non-LR), and stored (10 and 21 days) as pRBCs. Samples were collected at donation, and before and after a simulated transfusion. PS expression was measured by flow cytometry, and concentrations of arachidonic acid (AA), prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (6-keto-PGF1α) …

Use Of Software Modeling Tools To Understand Population Health Dynamics: Application To Bovine Respiratory Disease In Us Beef Calves Prior To Weaning, Min Wang

Theses and Dissertations

Bovine respiratory disease (BRD) is a significant health problem for cattle producers in terms of economic cost and animal welfare. In the United States (US), it is one of the leading causes of sickness and death in beef calves prior to weaning. Although much research has been conducted to develop vaccines for prevention and antibiotics for treatment, the morbidity and mortality of BRD in beef calves prior to weaning has not improved over the years. The identification of risk factors associated with BRD is an area of focus which might ultimately allow producers to minimize morbidity and mortality from BRD. …

In Vitro And In Vivo Effects Of An Encapsulated Butyric Acid And A Lactic Acid Producing Bacteria Used Alone Or In Combination, Dana Kristen Dittoe

Theses and Dissertations

Lactic acid producing bacteria (LAB) and organic acids have been proposed as effective alternatives to antibiotics, however there is limited literature concerning their combination. Thus, the objective of this thesis was to investigate the effects of LAB, encapsulated butyric acid (EBA), and their combination. Under challenged conditions, the in vitro study demonstrated that both EBA and LAB were capable of reducing pathogen proliferation over time, with greater efficacy at lower initial concentration. However, EBA demonstrated a greater effectiveness on pathogen reduction. Under challenged conditions, the results of the in vivo study indicated that birds fed LAB performed comparatively to that …

Genomics And Molecular Approaches To Delineate Pathogenesis Of Aeromonas Hydrophila, Aeromonas Veronii, And Edwardsiella Piscicida Infections In Fish, Hasan Cihad Tekedar

Theses and Dissertations

The U.S. aquaculture industry has become well established in the last three decades, and channel catfish aquaculture is the most significant component of this industry. Virulent Aeromonas hydrophila has been a serious disease problem since 2009 in the U.S. catfish aquaculture, and Aeromonas veronii and Edwardsiella piscicida are emerging pathogens of catfish. Therefore, this study aims to address fundamental questions on virulence mechanisms of these three fish pathogens, which I expect to support the development of control measures for preventing these diseases. In this study, E. piscicida and virulent Aeromonas hydrophila (vAh) genomes were sequenced, and comparative analyses were conducted …

Nanotechnology-Based Approaches For Imaging And Therapy, Charalambos Kaittanis

Amgen Seminar Series in Chemical Engineering

Improved therapeutics and diagnostic tools are needed in the clinic, contributing towards better treatment and survivorship. During this presentation, we will discuss how nanoparticle-based tools can support decision-making through imaging, and provide new insights on how nanoparticles interact with their environment. Overall, we will explore how nanoparticles can serve as sensitive probes, as well as translational platforms for personalized medicine.

Loss Of Sympathetic Nerves In Spleens From Patients With End Stage Sepsis, Donald B. Hoover, Thomas Christopher Brown, Madeleine K. Miller, John B. Schweitzer, David L. Williams

ETSU Faculty Works

The spleen is an important site for central regulation of immune function by noradrenergic sympathetic nerves, but little is known about this major region of neuroimmune communication in humans. Experimental studies using animal models have established that sympathetic innervation of the spleen is essential for cholinergic anti-inflammatory responses evoked by vagal nerve stimulation, and clinical studies are evaluating this approach for treating inflammatory diseases. Most data on sympathetic nerves in spleen derive from rodent studies, and this work has established that remodeling of sympathetic innervation can occur during inflammation. However, little is known about the effects of sepsis on spleen …

The Pul37 Tegument Protein Guides Alphaherpesvirus Retrograde Axonal Transport To Promote Neuroinvasion, Alexsia L. Richards, Patricia J. Sollars, Jared D. Pitts, Austin M. Stults, Ekaterina E. Heldwein, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

A hallmark property of the neurotropic alpha-herpesvirinae is the dissemination of infection to sensory and autonomic ganglia of the peripheral nervous system following an initial exposure at mucosal surfaces. The peripheral ganglia serve as the latent virus reservoir and the source of recurrent infections such as cold sores (herpes simplex virus type I) and shingles (varicella zoster virus). However, the means by which these viruses routinely invade the nervous system is not fully understood. We report that an internal virion component, the pUL37 tegument protein, has a surface region that is an essential neuroinvasion effector. Mutation of this region rendered …

Emergency Action Plan Policy Adoption In Secondary School Athletics, Samantha E. Scarneo

Doctoral Dissertations

Archival abstract submitted.

Lessons From The Fields: Female Farmworkers And The Law, Maria L. Ontiveros

Maine Law Review

In both the fields of labor law and gender studies, we learn the most from experience. The experience of workers coming together to demand equality and respect and the experience of women coming together to share their experiences has led to most of what we study in these fields. Unfortunately, too many times traditional legal doctrine does not fit these experiences. In those cases, we must struggle to change the law to be responsive to the lived experiences of women and workers. This Article explores the lived experiences of one particular group of workers—immigrant farmworking women in California. From their …

61st Conference On Senior Engineering Design, College Of Engineering And Applied Sciences

Senior Engineering Design Conference

The 61st Conference on Senior Engineering Design Projects. The conference was held from 9:00 a.m. to 2:00 p.m.,Tuesday, December 5, 2017 at the College of Engineering and Applied Sciences on the Parkview Campus of Western Michigan University. The College of Engineering and Applied Sciences sponsored the conference to showcase the work of its graduating seniors, who are required to complete a capstone project that puts into practice what they have learned. Many of the projects are sponsored by business and industry.

Running Into The Arms Of Expatriation: America's Failure Addressing The Rights Of Unaccompanied Migrant Children From Central America, Rebeca Garcia Gil

Maryland Journal of International Law

No abstract provided.

Relationship Between Self-Reported Oral Health And Self-Esteem In Two Columbia, Sc Populations, Matthew Ablonczy, Elizabeth Smith

Senior Theses

The importance of oral health to overall health cannot be overstated. For example, oral health can be used for early diagnosis of diseases such as diabetes. Furthermore, it has been well studied that self-esteem is related to oral health. Individuals with proper oral health care have more confidence to interact with others, which results in higher self-esteem. Those who lack the ability to pay for oral healthcare are thus at a great disadvantage. The present study sought to investigate the relationship between oral health and self-esteem in two Columbia, SC populations through surveying. There were two study populations, students at …

Severity Of Acute Infectious Mononucleosis Correlates With Cross- Reactive Influenza Cd8 T-Cell Receptor Repertoires, Nuray Aslan, Levi B. Watkin, Anna Gil, Rabinarayan Mishra, Fransenio G. Clark, Raymond M. Welsh, Dario Ghersi, Katherine Luzuriaga, Liisa K. Selin

Interdisciplinary Informatics Faculty Publications

Fifty years after the discovery of Epstein-Barr virus (EBV), it remains unclear how primary infection with this virus leads to massive CD8 T-cell expansion and acute infectious mononucleosis (AIM) in young adults. AIM can vary greatly in severity, from a mild transient influenza-like illness to a prolonged severe syndrome. We questioned whether expansion of a unique HLA-A2.01-restricted, cross-reactive CD8 T-cell response between influenza virus A-M158 (IAV-M1) and EBV BMLF1280 (EBV-BM) could modulate the immune response to EBV and play a role in determining the severity of AIM in 32 college students. Only ex vivo total IAV-M1 and IAV-M1+EBV-BM cross-reactive tetramer+ …

Blood Culture Contamination In The Emergency Department, Sharon Winters, Patricia Mcclure

General Submissions: Presentations (Oral and Poster)

Purpose: Current best practice for collecting blood cultures is to divert the first 1-2ml of blood, so to send the micro-organisms away from the blood culture tubes that will be tested. Residing in the deeper dermis layer of skin, are still 20% of common contaminate organisms such as MRSA and VRE, that do not go away even after scrubbing for 30 seconds with chlorahexadine and allowing it to dry completely. Drawing into syringes is not best practice, as we have found alongside with our micro department, that contaminates are being resulted in both the aerobic and anaerobic bottles from mixing …