Digital Commons Network^™

Knowledge Mobilisation For Policy Development: Implementing Systems Approaches Through Participatory Dynamic Simulation Modelling, Louise Freebairn, Lucie Rychetnik, Jo-An Atkinson, Paul Kelly, Geoff Mcdonnell, Nick Roberts, Christine Whittall, Sally Redman

Medical Papers and Journal Articles

Background: Evidence-based decision-making is an important foundation for health policy and service planning decisions, yet there remain challenges in ensuring that the many forms of available evidence are considered when decisions are being made. Mobilising knowledge for policy and practice is an emergent process, and one that is highly relational, often messy and profoundly context dependent. Systems approaches, such as dynamic simulation modelling can be used to examine both complex health issues and the context in which they are embedded, and to develop decision support tools.

Objective: This paper reports on the novel use of participatory simulation modelling as a …

Perceptions Of Recent Medical Graduates And Their Workplace Supervisors Towards A Medical School Clinical Audit Program, Stephanie Davis, Ilse O'Ferrall, Samuel Hoare, Caroline Bulsara, Donna B. Mak

Medical Papers and Journal Articles

Objectives: This study explores how medical graduates and their workplace supervisors perceive the value of a structured clinical audit program (CAP) undertaken during medical school.

Methods: Medical students at the University of Notre Dame Fremantle complete a structured clinical audit program in their final year of medical school. Semi-structured inter-views were conducted with 12 Notre Dame graduates (who had all completed the CAP), and seven workplace supervisors (quality and safety staff and clinical supervisors). Purposeful sampling was used to recruit participants and data were analysed using thematic analysis.

Results: Both graduates and workplace supervisors perceived the CAP to be valuable. …

Evidence For A Comprehensive Approach To Aboriginal Tobacco Control To Maintain The Decline In Smoking: An Overview Of Reviews Among Indigenous Peoples, Catherine Chamberlain, Susan Perlan, Sue Brennan, Lucie Rychetnik, David Thomas, Raglan Maddox, Noore Alam, Emily Banks, Andrew Wilson, Sandra Eades

Medical Papers and Journal Articles

Background: Tobacco smoking is a leading cause of disease and premature mortality among Aboriginal and Torres Strait Islander (Indigenous) Australians. While the daily smoking prevalence among Indigenous Australians has declined significantly from 49% in 2001, it remains about three times higher than that of non-Indigenous Australians (39 and 14%, respectively, for age ≥15 years in 2014–15). This overview of systematic reviews aimed to synthesise evidence about reducing tobacco consumption among Indigenous peoples using a comprehensive framework for Indigenous tobacco control in Australia comprised of the National Tobacco Strategy (NTS) and National Aboriginal and Torres Strait Islander Health Plan (NATSIHP) principles …

Can Peer Education Improve Beliefs, Knowledge, Motivation And Intention To Engage In Falls Prevention Amongst Community-Dwelling Older Adults?, Linda Khong, Richard G. Berlach, Keith D. Hill, Anne-Marie Hill

IHR Papers and Journal Articles

The aim of the study was to evaluate the effectiveness of delivering a contemporary peer-led falls prevention education presentation on community-dwelling older adults’ beliefs, knowledge, motivation and intention to engage in falls prevention strategies. A two-group quasi-experimental pre-test–post-test study using a convenience sample was conducted. A new falls prevention training package for peer educators was developed, drawing on contemporary adult learning and behaviour change principles. A 1-h presentation was delivered to community-dwelling older adults by peer educators trained with the new package (intervention group). Control group participants received an existing, 1-h falls prevention presentation by trained peer educators who had …

Effectiveness Of Peers In Delivering Programs Or Motivating Older People To Increase Their Participation In Physical Activity: Systematic Review And Meta-Analysis, Elissa Burton, Kaela Farrier, Keith Hill, James Codde, Phil Airey, Anne-Marie Hill

Health Sciences Papers and Journal Articles

The objective of this systematic review and meta-analysis was to evaluate the effectiveness of peers to deliver programs or encourage older people to be physically active and improve physical outcomes. Peer reviewed articles published in English between January 1976 and June 2016, retrieved from six databases according to the predefined inclusion criteria were included. Where possible results were pooled and meta-analyses conducted. Eighteen articles were included in the review, a total of 3,492 intervention participants, average age 66.5 years and 67.1% were female. Overall, study quality was medium to high. Interventions mainly included resistance, flexibility and cardiovascular training, however there …

Physical Determinants Of Division 1 Collegiate Basketball, Women’S National Basketball League And Women’S National Basketball Association Athletes: With Reference To Lower Body Sidedness, T Spiteri, M Binetti, A Scanlan, V Dalbo, F Dolci, C Specos

Health Sciences Papers and Journal Articles

In female basketball the assumed components of success include power, agility, and the proficiency at executing movements using each limb. However, the importance of these attributes in discriminating between playing levels in female basketball have yet to be determined. The purpose of this study was to compare lower body power, change of direction (COD) speed, agility, and lower-body sidedness between basketball athletes participating in Division 1 Collegiate basketball (United States), Women's National Basketball League (WNBL) (Australia), and Women's National Basketball Association (WNBA) (United States). Fifteen female athletes from each league (N = 45) completed a double and single leg counter-movement …

Patient Evaluation Of Emotional Comfort Experienced (Peece): Developing And Testing A Measurement Instrument, A Williams, L Lester, C Bulsara, A Petterson, K Bennett, E Allen, D Joske

Health Sciences Papers and Journal Articles

Objectives: The Patient Evaluation of Emotional Comfort Experienced (PEECE) is a 12-item questionnaire which measures the mental well-being state of emotional comfort in patients. The instrument was developed using previous qualitative work and published literature.

Design: Instrument development.

Setting: Acute Care Public Hospital, Western Australia.

Participants: Sample of 374 patients.

Interventions: A multidisciplinary expert panel assessed the face and content validity of the instrument and following a pilot study, the psychometric properties of the instrument were explored.

Main outcome measures: Exploratory and confirmatory factor analysis assessed the underlying dimensions of the PEECE instrument; Cronbach’s α was used to determine the …

Community-Based Prescribing For Impetigo In Remote Australia: An Opportunity For Antimicrobial Stewardship, S Oliver, J Cush, J Ward

Health Sciences Papers and Journal Articles

Background: To support antibiotic prescribing for both hospital and community-based health professionals working in remote North Western Australia, a multidisciplinary Antimicrobial Stewardship (AMS) Committee was established in 2013. This Committee is usually focused on hospital-based prescribing. A troubling increase in sulfamethoxazole/ trimethoprim resistance in Staphylococcus aureus antibiograms from 9 to 18% over 1 year prompted a shift in gaze to community prescribing.

What we did: Finding a paucity of relevant research, we first investigated contextual factors influencing local prescribing. We also designed a systematic survey of experts with experience relevant to our setting using a structured response survey (12 questions) …

Lack Of Evidence For Decreased Protein Stability In The 2397 (Met) Haplotype Of The Leucine Rich Repeat Kinase 2 Protein Implicated In Parkinson’S Disease, Ryan S. Anderton, Lauren Hill, Rhiannon Morris, Frank L. Mastaglia, Wayne Greene, Sherif Boulos

Health Sciences Papers and Journal Articles

Missense mutations in the leucine rich repeat kinase 2 (LRRK2) gene are the leading genetic cause of autosomal dominant familial Parkinson’s disease. We previously reported that two mutations within the ROC domain, namely R1441C and A1442P, exhibit increased protein degradation leading to lowered steady state LRRK2 protein levels in HEK293 cells. More recently, the common WD40 domain LRRK2 haplotype, Met2397, which is a risk factor for Crohn’s disease, has been shown to lower steady state protein levels in HEK293 cells. In view of recent evidence implicating LRRK2 and inflame-mation in PD, we investigated the effects of Met2397 on LRRK2 expression, …

Changes In Fatty Acid Composition Of Human Milk In Response To Cold-Like Symptoms In The Lactating Mother And Infant, Andrew S. Gardner, Ibrahim A. Rahman, Ching T. Lai, Anna Hepworth, Naomi Trengove, Peter E. Hartmann, Donna T. Geddes

Health Sciences Papers and Journal Articles

Infants rely on their innate immune systems to protect them from infection. Human milk (HM) contains fatty acids (FAs) and monoacylglycerols that are known to exhibit antiviral and antibacterial properties in vitro. The specific fat content of HM may potentially affect the efficacy of this antimicrobial activity. This preliminary study investigates whether the proportions of FA in HM change in response to infections, leading to cold-like symptoms in the mother or infant. Milk samples were obtained from mothers (n = 26) when they and their infants were healthy, and when mother, infant, or both suffered cold-like symptoms. The milk was …

Eeg Neurofeedback For Executive Functions In Children With Neurodevelopmental Challenges, Jasmin K. Landes, Corinne L. Reid, Martijn Arns, Nicholas A. Badcock, Tomas Ros, Stefanie Enriquez-Geppert, Max K. Bulsara, Stefano Brini, Sheida Rabipour, Mimma Mason, Neils Birbaumer, Bethanie Gouldthorp, Mike Anderson

Health Sciences Papers and Journal Articles

This is a protocol for a Cochrane Review (Intervention). The objectives are as follows:

To assess the effectiveness of EEG neurofeedback as treatment for inhibition and updating problems in children facing neurodevelopmental challenges.

Development Of A Theory-Informed Implementation Intervention To Improve The Triage, Treatment And Transfer Of Stroke Patients In Emergency Departments Using The Theoretical Domains Framework (Tdf): The T3 Trial, Louise E. Craig, Natalie Taylor, Rohan Grimley, Dominique A. Cadilhac, Elizabeth Mcinnes, Rosemary Phillips, Simon Dale, Denise O'Connor, Chris Levi, Mark Fitzgerald, Julie Considine, Jeremy M. Grimshaw, Richard Gerraty, N. Wah Cheung, Jeanette Ward, Sandy Middleton

Health Sciences Papers and Journal Articles

Background: Theoretical frameworks and models based on behaviour change theories are increasingly used in the development of implementation interventions. Development of an implementation intervention is often based on the available evidence base and practical issues, i.e. feasibility and acceptability. The aim of this study was to describe the development of an implementation intervention for the T3 Trial (Triage, Treatment and Transfer of patients with stroke in emergency departments (EDs)) using theory to recommend behaviour change techniques (BCTs) and drawing on the research evidence base and practical issues of feasibility and acceptability.

Methods: A stepped method for developing complex interventions based …

Neuromuscular Training Improves Movement Competency And Physical Performance Measures In 11-13 Year Old Female Netball Athletes, A Hopper, E Haff, O Barley, C Joyce, R Lloyd, G Haff

Health Sciences Papers and Journal Articles

The purpose of this study was to examine the effects of a neuromuscular training program on movement competency and measures of physical performance in youth female netball players. It was hypothesized that significant improvements would be found in movement competency and physical performance measures following the intervention. Twenty-three junior female netball players (age, 12.17 ± 0.94 yrs; height, 1.63 ± 0.08 m; weight, 51.81 ± 8.45 kg) completed a test battery before and after a six-week training intervention. 13 of these athletes underwent six weeks of neuromuscular training, which incorporated plyometrics and resistance training. Trained athletes showed significant improvements in …

Reducing Falls After Hospital Discharge: Protocol For A Randomised Controlled Trial Evaluating An Individualised Multi-Modal Falls Education Program For Older Adults, A. Hill, C. Etherton-Beer, S. Mcphail, M. Morris, L. Flicker, R. Shorr, M. Bulsara, D. Lee, J. Francis-Coad, N. Waldron, A. Boudville, T. Haines

Health Sciences Papers and Journal Articles

Introduction: Older adults frequently fall after discharge from hospital. Older people may have low self-perceived risk of falls and poor knowledge about falls prevention. The primary aim of the study is to evaluate the effect of providing tailored falls prevention education in addition to usual care on falls rates in older people after discharge from hospital compared to providing a social intervention in addition to usual care.

Methods and analyses: The ‘Back to My Best’ study is a multisite, single blind, parallel-group randomised controlled trial with blinded outcome assessment and intention-to-treat analysis, adhering to CONSORT guidelines. Patients (n=390) (aged 60 …

Birth Experience Of Fathers In The Setting Of Teenage Pregnancy: Are They Prepared?, Simeon Ngweso, Rodney W. Petersen, Julie Quinlivan

Health Sciences Papers and Journal Articles

Aim: To explore the birth experiences of teenage fathers and determine the extent to which they are prepared for childbirth.

Methods: A mixed methods observational study was undertaken comparing the birth experience of 50 fathers in the setting of teenage pregnancy (teenage) compared to a group of 50 older fathers. Fathers were recruited in the antenatal period and completed structured questionnaires following the birth of their child. Quantitative and qualitative analysis was undertaken.

Results: Teenage fathers were younger, less educated and less likely to attend prenatal childbirth education classes (P < 0.0001). During birth, they were less prepared and consulted by attending staff (both P < 0.05). They reported limited roles in intrapartum decision-making (< 20%). In multivariate analysis being a father in the setting of teenage pregnancy remained significantly associated with feeling unprepared for birth. The major themes in qualitative analysis were feeling unprepared, shock, fear, a sense of detachment, happiness, pride, love of the baby and satisfaction with fertility.

Conclusion: Teenage fathers are less prepared for the …

Take A Break! Benefits Of Sleep And Short Breaks For Daily Work Engagement, Jana Kuhnel, Hannes Zacher, Jessica De Bloom, Ronald Bledow

Research Collection Lee Kong Chian School Of Business

The current study investigates the benefits of a good night’s sleep and short work breaks for employees’ daily work engagement. It is hypothesized that sleep and self-initiated short breaks help restore energetic and self-regulatory resources which, in turn, enable employees to experience high work engagement. A daily diary study was conducted with 107 employees who provided data twice a day (before lunch and at the end of the working day) over 5 workdays (453 days in total). Multilevel regression analyses showed that sleep quality and short breaks were beneficial for employees’ daily work engagement. After nights employees slept better, they …

The Plant As A Habitat For Entomophagous Insects, L. Kaiser, P. Ode, S. Van Nouhuys, P. A. Calatayud, S. Colazza, A. M. Cortesero, A. Thiel, J. Van Baaren

All Peer-Reviewed Publications

Populations of herbivorous insects are naturally consumed by other predacious or predatory insect species. These entomophagous insects are thus plant-dwelling organisms that use the plant for several vital functions and are affected by plant traits at the evolutionary, organism and population levels. Many entomophagous species are used for the biological control of insect pests worldwide. The aim of this chapter is to provide an exhaustive review of mechanisms underlying the interactions between plants and entomophagous insects, including those governing life history traits at the individual level, as well as those acting on population and community structure and dynamics. We detail …

Use Of Insects For Fish And Poultry Compound Feed In Sub-Saharan Africa - A Systematic Review, G. Ssepuuya, V. Namulawa, D. Mbabazi, S. Mugerwa, P. Fuuna, Z. Nampijja, S. Ekesi, K. K.M. Fiaboe, D. Nakimbugwe

All Peer-Reviewed Publications

The cost of compound feed is a constraint in intensive fish and poultry farming, contributing 60-80% of the total production costs, 70% of which is due to fish and soy meal used as protein source. This review presents the extent to which insects have been utilised as an alternative protein source in feed in sub-Saharan Africa (SSA). A key word search of agricultural, biological and multi-disciplinary databases and academic search engines was conducted for literature on the extent of research and utilisation of insects in poultry and fish feed in SSA. There is limited published information on the practice as …

Steinernema Pwaniensis N. Sp., A New Entomopathogenic Nematode (Nematoda: Steinernematidae) From Tanzania, V. Půža, J. Nermut, Z. Mráček, S. Gengler, S. Haukeland

All Peer-Reviewed Publications

A new species of entomopathogenic nematode, Steinernema pwaniensis n. sp., belonging to the glaseri group, is described from Tanzania. The infective juveniles of S. pwaniensis n. sp. are characterized by a body length of 978 μm (808-1131), distance from anterior end to excretory pore of 86 μm (80-95) and a tail length of 87 μm (75-95). The ratios a, c and E% of S. pwaniensis n. sp. are substantially lower than those of all other African 'glaseri' group members. The first-generation males of S. pwaniensis n. sp. can be distinguished by a large spicule length of 92 μm (80-97) and …

Angiostoma Norvegicum N. Sp. (Nematoda: Angiostomatidae) A Parasite Of Arionid Slugs In Norway, Jenna L. Ross, Solveig Haukeland, Bjørn A. Hatteland, Elena S. Ivanova

All Peer-Reviewed Publications

Angiostoma norvegicum n. sp. (Angiostomatidae) is described from the oesophagus, crop and the buccal mass of five species of slugs of the family Arionidae, Arion vulgaris (Moquin-Tandon), Arion ater (L.), Arion fasciatus (Nilsson), Arion fuscus (Müller) and Arion rufus/Arion ater hybrid), collected throughout Norway. Angiostoma norvegicum n. sp. was found parasitising arionids at seven of the 30 sample sites examined (23.3%), and 9.9% of all Arion spp. were infected with this nematode. The new species is characterised by its large size (4.0–8.6 mm long) and in having: lateral alae; 6 + 6 papillae at the cephalic end; a large circular …

Entomopathogens Routinely Used In Pest Control Strategies: Orchards In Tropical Climate, N. K. Maniania, S. Ekesi, C. Dolinski

All Peer-Reviewed Publications

Fruit and nut trees such as mango, pineapples, papaya, banana, guava, cashew, passion fruit, and avocado are important components of tropical ecosystems. They are sources of vitamins, minerals and carbohydrates and have great economic importance as sources of both household incomes and national revenues. Fruit and nut trees provide important adaptive values and tend to be more resilient to climate change due to their perennial nature, and they have the property of sequestering carbon. World production and trade of fresh tropical fruit are expected to expand over decades, with developing countries accounting for about 98% of total production, while developed …

Patient Predictors Of Poor Drug Sensitive Tuberculosis Treatment Outcomes In Kyiv Oblast, Ukraine, Omowunmi Aibana, Andrej Slavuckij, Mariya Bachmaha, Viatcheslav Krasiuk, Natasha Rybak, Timothy P Flanigan, Vasyl Petrenko, Megan B Murray

Faculty, Staff and Student Publications

Background: Ukraine has high rates of poor treatment outcomes among drug sensitive tuberculosis (DSTB) patients, while global treatment success rates for DSTB remain high.

We evaluated baseline patient factors as predictors of poor DSTB treatment outcomes.

Methods: We conducted a retrospective analysis of new drug sensitive pulmonary TB patients treated in Kyiv Oblast, Ukraine between November 2012 and October 2014. We defined good treatment outcomes as cure or completion and poor outcomes as death, default (lost to follow up) or treatment failure. We performed logistic regression analyses, using routine program data, to identify baseline patient factors associated with poor outcomes. …

Genetic Diversity And Population Structure Of The African Catfish, Clarias Gariepinus (Burchell, 1822) In Kenya: Implication For Conservation And Aquaculture, James E. Barasa, Sinebongo Mdyogolo, Romulus Abila, Johannes Paul Grobler, Robert A. Skilton, Hesmari Bindeman, Moses Ndotono Njahira, Emily J. Chemoiwa, Otto G. Dangasuk, Boaz Kaunda-Arara, Erik Verheyen

All Peer-Reviewed Publications

African catfish, Clarias gariepinus, is an important species in aquaculture and fisheries in Kenya. Mitochondrial D-loop control region was used to determine genetic variation and population structure in samples of C. gariepinus from 10 sites including five natural populations (Lakes Victoria (LVG), Kanyaboli (LKG), Turkana (LTA), Baringo (LBA) and Jipe (LJP), and five farms (Sangoro Aquaculture Center (SAN), Sagana Aquaculture Centre (SAG), University of Eldoret Fish Farm (UoE), Kibos Fish Farm (KIB), and Wakhungu Fish Farm (WKU)) in Kenya. Similarly, samples from eight localities (four natural populations: LVG/LKG, LTA, LBA, and four farmed: SAN, SAG, KIB, UoE) were genotyped using …

Dna Barcodes, Expanded Distribution, And Redescription Of Apanteles Hemara Nixon, 1965 (Hymenoptera, Braconidae, Microgastrinae), A Potential Biocontrol Species Against Amaranth Leaf-Webbers In Africa, Jose Fernandez-Triana, Melanie Beaudin, Kees Van Achterberg, Mawufe K. Agbodzavu, Stephen T.O. Othim, Faith W. Nyamu, Komi K.M. Fiaboe

All Peer-Reviewed Publications

The microgastrine parasitoid wasp Apanteles hemara Nixon, 1965, is currently being considered as a potential biocontrol agent of amaranth leaf-webber pests in Africa. To facilitate future research and identification of the species, we characterize it from an integrative taxonomy perspective by providing a comprehensive morphological redescription, extensive illustrations (including the first images of the holotype), DNA barcodes, wasp biology, host data (Choreutidae and Crambidae caterpillars), and updated geographical distribution of the species (including eight new country records). Despite a wide distribution across four major biogeographical regions (mostly within the Old World tropics), the species seems to be relatively uniform from …

Systematics And Biology Of Cotesia Typhae Sp. N. (Hymenoptera, Braconidae, Microgastrinae), A Potential Biological Control Agent Against The Noctuid Mediterranean Corn Borer, Sesamia Nonagrioides, Laure Kaiser, Jose Fernandez-Triana, Claire Capdevielle-Dulac, Célina Chantre, Matthieu Bodet, Ferial Kaoula, Romain Benoist, Paul André Calatayud, Stéphane Dupas, Elisabeth A. Herniou, Rémi Jeannette, Julius Obonyo, Jean François Silvain, Bruno Le Ru

All Peer-Reviewed Publications

Many parasitoid species are subjected to strong selective pressures from their host, and their adaptive response may result in the formation of genetically differentiated populations, called host races. When environmental factors and reproduction traits prevent gene flow, host races become distinct species. Such a process has recently been documented within the Cotesia flavipes species complex, all of which are larval parasitoids of moth species whose larvae are stem borers of Poales. A previous study on the African species C. sesamiae, incorporating molecular, ecological and biological data on various samples, showed that a particular population could be considered as a distinct …

Holistic Self-Care: Tai Chi, Qigong Training For Caregivers At An Alzheimer Disease Assisted Living Facility, Sunny Alperson, Christianne Fowler

Ellmer School of Nursing Faculty Publications

With the growing number of older persons with chronic illnesses, the demand for professionals who can provide complex care is increasing as well. However, evidence shows that health professionals’ burnout rate has been consistently increasing for the last 3 decades. In an effort to alleviate some of the issues associated with caregiver burnout, we conducted mind-body training of self-compassion for professional caregivers at an Alzheimer disease (AD) specialty center for 5 weeks. The intervention combined seven simple centering movements of tai chi and qigong in a context of mettā (loving kindness meditation) language mindfulness. Data showed significant improvements in caregiver …

Heme Oxygenase 1 Expression After Traumatic Brain Injury And Effect Of Pharmacological Manipulation On Functional Recovery., Nicholas H. Russell

Theses and Dissertations

Traumatic Brain Injury (TBI) is an increasingly diagnosed constellation of injuries derived from acute mechanical trauma to the brain. With the rise of advanced neuroimaging techniques recent focus has oriented primarily towards the mild-moderate range of TBI which previously was missed diagnostically. Characteristically, these advances have shown increasing areas of micro-hemorrhage in susceptible areas of the brain and to date there are no treatment modalities targeting micro-hemorrhages or their sequelae. This dissertation explores the effects of the resulting heme processing response in the days following injury with a particular focus on inducing early heme clearance from the parenchyma using a …

Implementing Integrated Care In Family Medicine: Description And Outcomes In An Underserved Population, Zach Radcliff

Theses and Dissertations

Introduction: Family physicians provide access to medical and behavioral healthcare for many underserved populations. Integrating behavioral health clinicians into primary care practices has been proposed as one of the most effective ways to increase access to necessary behavioral health services for many Americans. Integrated behavioral healthcare (IBHC) has begun to be implemented in family medicine practices but there is limited research examining the impact for patients and clinic staff. This study begins to fill this gap in the literature by examining the effects of implementing integrated behavioral healthcare in an urban family medicine clinic in a medically underserved area.

Objective: …

Modified Pamam Dendrimers In Tunable Drug-Delivery Systems: A Sustained-Release Dendrimer Hydrogel For Anti-Glaucoma Drugs And Surface-Engineered Macrophages As Nanoparticle Carriers For Targeted Anti-Cancer Therapy, Christopher A. Holden

Theses and Dissertations

Two specific drug-delivery applications were sought in this work using polyamidoamine (PAMAM) dendrimers. One drug-delivery system used a novel dendrimer hydrogel (DH) for sustained delivery of anti-glaucoma drugs. In this work, PAMAM G3.0 dendrimers were covalently bonded with poly(ethylene glycol) (PEG­₁₂₀₀₀) molecules which were subsequently acrylated, resulting in photocurable DH conjugates. For pharmacological studies, DH were loaded with a solution of intraocular pressure lowering drugs, brimonidine and timolol maleate, and were characterized for in vitro release and ex vivo transport and uptake. DH formulations were shown to increase the loading of drug molecules, increase transcorneal drug delivery, and …

Pnpase In C. Elegans: Mutagenic Analysis To Complement Knockdown Studies, Danielle K. Seibert

Theses and Dissertations

PNPase is a gene implicated as a potential target for cancer therapy; human mutations also present with deafness, myopathies, and neuropathies. In this study, C. elegans was used to investigate the effect of knocking out PNPase in a whole animal. C. elegans knockdown studies have reported an extended lifespan via an increase in ROS production. Further noted are larger mitochondria and an increase in fzo-1 expression. Knockout animals previously constructed using CRISPR/Cas9 were used for this study. We aimed to confirm these findings validating previous studies. It was discovered that PNPase knockout animals demonstrated a similar lifespan extension that was …